Food Science Commons^™

Blueberry Pectin-Anthocyanin Binding Kinetics And Stability Under In Vitro Digestion, Jee Won Koh

LSU Doctoral Dissertations

Blueberry pectins were sequentially extracted from highbush blueberry powder and precipitated in alcohol to obtain water soluble fraction (WSF) and chelator soluble fraction (CSF). WSF was high methoxyl pectin (degree of esterification: 53), rich in in neutral sugars (40 mol%), and had less negative charge (-21 mV) whereas CSF was low methoxyl pectin (degree of esterification: 21), rich in uronic acids (92 mol%), and had lower negative charge ( -26 mV).

The stability of blueberry pectin-anthocyanin complex was investigated under simulated in vitro digestion. Binding of blueberry pectins with three anthocyanin standards (malvidin-3-glucoside; M3G, cyanidin-3-glucoside; C3G, and delphinidin-3-glucoside; D3G) and …

Sensory Analysis For Determining The Influence Of Visual Stimuli Upon Eating Experiences, Pitchayapat Chonpracha

LSU Doctoral Dissertations

Consumers are currently more conscious about salt and sugar intake than they were in the past. Visual cues have been shown to impact consumers’ cognitive and their taste perceptions. Current research information about visual cues enhancing human’s tastes expectation of saltiness and sweetness is limited. The sophisticated sensory analysis for products containing a reduced amount of salt and sugar is undoubtedly time–consuming and costly if the sensory experiment is designed with many samples. Therefore, the following three studies were achieved: I) an examination of how intrinsic and extrinsic visual cues affect the overall consumer eating experiences; II) a comparison of …

Effect Of Environmental Factors And Residual Sanitizers On Survival And Attachment Of Indicator Organism And Human Pathogens On Fresh Produce, Vijay Singh Chhetri

LSU Doctoral Dissertations

A comprehensive understanding of the behavior of microbial contaminants on produce surfaces in agricultural farms and during post-harvest activities is essential for the development of produce safety risk management strategies. Our studies investigated the survival and the attachment of E. coli on watermelon surfaces in agricultural settings with different levels of vegetation, and the effect of residual sanitizers on the survival and the attachment of E. coli O157: H7 and L. monocytogenes on spinach leaves. The attachment strength (S_R) of the E. coli cells on watermelon surfaces significantly increased (P<0.05) from 0.04 to 0.99 in the first 24 h, which was primarily due to the decrease in loosely attached population, given that the population of strongly attached cells was constant. The daily die-off rate of E. coli ranged from -0.12 …

Effects Of Red Raspberry Polyphenols And Metabolites On Biomarkers Of Inflammation And Insulin Resistance In Prediabetes And Type 2 Diabetes, Reynaldo Moreno Ucles

LSU Doctoral Dissertations

Berry fruits are rich sources of polyphenolic compounds (PCs), which can promote health benefits. Anthocyanin concentrations of red raspberry (RR)(Rubus idaeus) extracts were determined to be a total of 887.6 ± 262.8 μg/g cyanidin-3-sophoroside (C3S) equivalents with C3S being the most prevalent. Ellagitannin analysis by MALDI-TOF indicated sanguiin H-6 and lambertianin C were the major ellagitannins present in RR. To explore the efficacy of RR in modulating diabetes, seven type 2 diabetes mellitus (T2DM) and two pre-diabetic patients were given drinks delivering one RR serving (123 g) per day in a smoothie for two weeks. Blood samples were …

Almond Milk Powder Fortification With Flaxseed Oil (Linum Usitatissimum) As An Alternative Non-Dairy Milk Powder For Infants And Toddlers, Federico Bueno

LSU Master's Theses

Lactose intolerances in toddlers have become a major concern. Almond milk (AM) has nutritional benefits and a texture like dairy milk without the content of lactose. Because of longer stability and storage, milk is often stored in powdered form. An almond milk fortified with flaxseed oil in powdered form may be an alternative. Flaxseed oil (FO) contains high amounts of alpha-linolenic acid (ALA) which is a precursor to DHA and EPA. The objectives of this study were: the development of an AM spray-dried powder with microencapsulated FO (AMFOP), the determination of the mass and energy balance of that AMFOP production, …

Influences Of Spiciness And Visual Color Cue On Salty Taste Intensity Perception In Reduced-Sodium Cheese Dips, Valentina Rosasco Silva

LSU Master's Theses

The current high levels of dietary sodium intake are negatively affecting consumers’ health. Reducing sodium consumption to the recommended amount could help prevent around 2.5 million deaths yearly and save up to $18 billion dollars in health care costs worldwide. The main objective of this research was to evaluate the possibility of increasing the saltiness perception and acceptability of reduced-sodium cheese dips by using the multisensory approach and analyzing gender differences towards salty taste expectations. Two experiments were conducted: I) The combined effects of gender and visual color cue on salty taste expectations, liking, and purchase intent (PI) of cheese …

Synthesis And Characterization Of Polyaniline Agglomerates And Polyaniline Nanoparticles To Use As Printed Sensors To Detect Spoilage Of Catfish During Refrigerated Storage, Vashti Martae Campbell

LSU Master's Theses

There is growing interest in the development of sensors contained in packaging for seafood that are applicable at the consumer level to monitor freshness in a non-destructive manner. Total volatile basic nitrogen (TVB-N) analysis indicates the freshness of seafood. TVB-N analysis is sensitive to the spoilage compounds comprised of ammonia, trimethylamine, dimethylamine, and biogenic amines commonly found in seafood and whose concentration increases when spoilage microbial growth occurs. However, TVB-N analysis is laborious and is not suitable for use at the consumer level. Polyaniline (PANI) is a polymer that may be used as a sensor for detecting TVB-N in seafood. …

Evaluation Of Microbial Safety And Quality Of Louisiana Strawberries After Flooding, Shifa Shiraz

LSU Master's Theses

Rainfall and flooding have impacted the strawberry industry in Louisiana. Floodwater is an ideal medium for microbiological growth and may cause contamination of soil, agricultural water, and fresh produce with foodborne pathogens. This research aimed to evaluate the microbial safety and quality of strawberries after flooding. Three strains of generic Escherichia coli were used to establish a baseline population of approximately 10⁶ CFU/L (high contamination) and 10² CFU/L (low contamination) in floodwater. Five raised beds were filled with water to simulate a flooding event. Simulated floodwater was mixed with cow manure and spiked with generic E. coli then …

Survival And Inactivation Of Salmonella Enterica And Human Norovirus On Consumer Contact Surfaces, Dorra Simmons

LSU Doctoral Dissertations

Salmonella enterica and human norovirus (HuNoV) are the leading cause of bacterial and viral gastroenteritis worldwide, respectively. One of the most important transmission routes of these pathogens is contaminated surfaces. In this study, we investigated the survival and the disinfection of Salmonella enterica serovar Typhimurium (S. Typhimurium) and HuNoV on selected consumer contact surfaces.

In the case of S. Typhimurium, formica laminate, stainless steel, and plastic cutting board surfaces were inoculated with different microbial loads of cells collected at log (6 h), stationary (24 h), or LTS (14 days) phases. The disinfection efficacy of two disinfectants (sodium hypochlorite or …

Delivering Entrapped Lactobacillus Plantarum Ncimb 8826 With Cereal-Legume Infant-Weaning Food To Improve Children's Gastrointestinal Health, Emmanuel Kyereh

LSU Doctoral Dissertations

Diarrhea remains the second leading cause of death among children under five globally, about one in five children’s deaths are attributable to it. In recent years,’ probiotics have been used as an alternative therapy measure to manage the situation. A promising delivery method that entraps probiotics in infant formula may help prevent or reduce diarrhea. The overall objectives of this study were to deliver entrapped Lactobacillus Plantarum NCIMB 8826 with maize-legume fortified complementary weaning food to reduce diarrhea and improve the gut microbiota. Both in vitro and in vivo models were used in this study and a mouse (C57BL/6 mice …

Whole Tart Cherry Improves Disease Activity Index In Rat Model Of Dextran Sulfate Solution – Induced Ulcerative Colitis By Downregulating The Janus 1 And Janus 3 Kinases And Tnf - Alpha, Johana Alexandra Coronel

LSU Master's Theses

Ulcerative colitis (UC) is a disabling inflammatory bowel disease. UC is characterized by chronic, relapsing inflammation of the colon and rectum. Current treatments such as monoclonal antibodies against TNF-α, IL-6, IL-12/p40, adhesion molecules, Janus kinases (JAK) inhibitors have side effects or lose their effects over time. Alternative approaches with fewer side effects for patients are needed. Montmorency tart cherries (Prunus cerasus) are a good source of anti-inflammatory flavonoids. We hypothesized that regular consumption whole tart cherry (TC) standardized to its major anthocyanin content, namely cyanidin-3-glucosyl-rutinoside, would be effective in reducing inflammation in UC. The aim of this research was to …

Insufficient Dha Intake In Pregnant And Lactating Women Of Baton Rouge, Louisiana: Further Nutrition Education Needed, Zoe Wilhelm

Honors Capstones

No abstract provided.

Antimicrobial Spice Cinnamon At Low Concentrations Has No Effect On The Counts Of Lactococcus Lactis R 604, But At High Concentrations Lowered Its Counts, Madison Hannan

Honors Capstones

No abstract provided.

Development, Acceptability, And Emotional Responses Of Low-Sodium Roasted Peanuts And Flavored Spreads, Kairy Dharali Pujols Martinez

LSU Doctoral Dissertations

The objective of this research was to evaluate the feasibility of developing acceptable low-sodium products by using a salt-replacement technique, an oil-in-water emulsion system, and health benefit information to improve consumers’ awareness of low-sodium diets. Three experiments were conducted: (I) Sensory characteristics and optimization of low-sodium roasted peanuts by substitution of NaCl with KCl, and addition of glycine (Gly) as a bitterness blocker; (II) Rejection threshold (RjT) of KCl added to low-sodium roasted peanuts using a 2AC-test; (III) Development of acceptable low-sodium and sodium-free spreads by flavor modification and their use on turkey salad sandwiches. In study I, results showed …

Interaction Of Cocoa Powder With Intestinal Microbiota, Zuyin Li

LSU Doctoral Dissertations

Cocoa powder contains polyphenolic compounds, which can provide potential health benefits to humans. The human gastroinestinal tract hosts diverse microorganisms which are called the human gut microbiota. These microbes can utilize carbohydrates through fermentation resulting in the production of short chain fatty acids (SCFAs), which cause a reduction of pH in gastroinestinal tract. The cocoa processing can change the composition of the carbohydrates, which can affect the fermentation patterns.

In this study we established an in vitro model system which simulated gastric digestion and colon fermentation. Five different processed cocoa samples (alkalized cocoa samples: D-11-B and D-11-S; non-alkalized cocoa samples: …

Evaluating The Effect Of Chitosan, Water-Soluble Chitosan And Chitosan Application Techniques On The Quality Of Refrigerated Catfish Fillets, Franklin Bonilla Torres

LSU Master's Theses

Chitosan (CH) is a biopolymer synthetized by deacetylation of chitin, it is found in the exoskeleton of crustaceans, cell wall of fungi, and insects. CH’s water insolubility limited the used of this compound in some food systems. However, enzymatic hydrolysis of CH yields water-soluble chitosan (WSC). The aim of this study was to evaluate the effect of chitosan, water-soluble chitosan, and chitosan application techniques on the quality of refrigerated catfish fillets. This research was conducted in two separated studies. In the first study, three solution treatments were prepared: (1) a 1% acetic acid (AA) solution, (2) a 0.5% CH solution …

Effect Of Hot Water Conditioning On Microbial Safety And Quality Of In-Shell Pecans, Karuna Kharel

LSU Master's Theses

Hot water conditioning of in-shell pecans is one common practice followed by industries to facilitate shelling. It also acts as a preventive control for potential microbiological contamination that might occur during pre- and post-harvest processes. However, heat treatment may have an effect on the eating quality of nuts. The main objectives of the study were to develop a post-harvest hot water treatment intervention as a kill step to destroy foodborne pathogens on in-shell pecans and evaluate the effect of treatments on physicochemical properties, consumer acceptance and purchase intent of dehulled and roasted pecans. The time (1-5 min) and temperature (70, …

Heat, Acid And Osmotic Stress Tolerance Of Leuconostoc Mesenteroides As Influenced By Prior Exposure To Various Mild Stress Conditions, Ingrid C. Osorio Rodriguez

LSU Master's Theses

The importance of Leuconostoc mesenteroides is recognized for its contribution to taste in cultured dairy products. It has been cited for its potential role as a probiotic. Consumer demand exists for new dairy products with health benefits. In the manufacture of probiotic-products survival of Leuconostoc mesenteroides would depend on its ability to tolerate challenging processing and storage conditions. Improved viability by prior exposure to mild stresses can increase stress tolerance toward a more severe stress. Furthermore, it can result in cross-protection due to connection of several proteins in response to stresses. Objective was to evaluate the effect of prior …

Interaction Of Cocoa Powder With Intestinal Microbiota, Martha M. Escoto Sabillon

LSU Master's Theses

Cocoa is the fully fermented and dried seed of the cacao tree (Theobroma cacao L.) which has prebiotic properties, due to their high concentration of polyphenols. Therefore, the ingestion of cocoa could cause changes in the proportions of the intestinal microbiota that can influence the intestinal immune response. The objective of this study was to determine the effect of alkalization process of the cocoa bean in the diversity of the gut microbiota. The samples were “lavado” unprocessed cocoa powder, “natural” unprocessed cocoa powder, “D-11-S” as alkalized cocoa powder, “D-11-B” heavily alkalized cocoa powder, and raw cocoa “shells” and a control …

Statistical And Technical Methodologies For Duplicated Multiple-Samples Preference And Attribute Intensity Sensory Ranking Test, Kennet Mariano Carabante Ordonez

LSU Doctoral Dissertations

Ranking tests are important preference and attribute difference tools for sensory evaluation. Replicated testing is used widely to reduce the number of panelists required in other sensory methods such as discrimination. The information regarding replications sensory ranking is limited. This research evaluated important statistical and technical aspects for the development of the foundation for duplicated sensory ranking tests. Three studies were accomplished: 1) A study of nonparametric analyses on real preference ranked data; 2) a sensitivity study of two samples serving protocols for duplicated visual ranking, and 3) protocols comparison in taste. In study 1, 125 panelists ranked in duplicates …

Isolation, Detection, And Prevention Of Foodborne Bacteria In Irrigation Water And Dairy Food, Mohammed Saleemallah Alhejaili

LSU Doctoral Dissertations

Foodborne microorganisms can be beneficial to humans or cause disease. Salmonella is one of the most causative foodborne disease outbreaks in the U.S.A. Salmonella has been detected in surface water used in irrigation, and can survive for weeks to years in water. The purpose of the study was to detect low levels of Salmonella spp. by comparing surfactant modified zeolite (SMZ) filtration and Activated Carbon (AC) filtration with the Environmental Protection Agency (EPA) method 1200. Also, to determine the generic E.coli counts and if there was a direct correlation of Salmonella to generic E. coli in irrigation water. The EPA …

A Preliminary Study To Evaluate Issues Involved Under In Vivo Conditions Of A Delivery System Containing Immobilized Lactobacillus Plantarum Ncimb 8826 On Rice Bran Fiber Extract, Chen Liu

LSU Master's Theses

Gut diseases like diarrhea, irritable bowel symptoms, and inflammatory bowel disease (IBD) can be treated with probiotics therapy. Probiotics have been recognized for their ability to promote the development of gut health and improve immune system. However, a difficulty involved with the use of probiotics is the need for and the ability to deliver a sufficient concentration of viable probiotic to the appropriate site. This can be difficult since the most common (and probably easiest) method of delivering the probiotic is orally. As such, the probiotic must be capable of surviving the hostile conditions of passage through the G.I. tract. …

Discovering Potential Protein, Carbohydrate, And Lipid Based Food Ingredients In A Co-Culture Of Microalgae, Chelsea Tyus

LSU Doctoral Dissertations

Louisiana Native Co-Culture of Microalgae (Chlorella Vulgaris L. and Cyanobacteria Leptolyngbya sp.) (CCA) was studied. CCA is a viable coculture for further investigation as a food component. This research characterized the proteins, carbohydrates, and lipids present in CCA. Algae cultivation parameters were controlled and analyzed. Treatment (Trt) 1 was CCA grown in cultures exposed to average scalar irradiance (ASI) of 1041 ± 269.18 μmol m-2 s-1 PAR and trt 2 was CCA grown in cultures exposed to ASI of 430 ± 96.03 μmol m-2 s-1 PAR. The trt (irradiance exposure) had the desired response on CCA species ratio Trt …

Failure To Achieve Recommended Omega-3 Dha Intake During Pregnancy: Nutrition Education Needed, Carly Thaxton

Honors Capstones

No abstract provided.

A Comparison Of Nutrient Intake, Nutrient Adequacy, Diet Quality, And Obesity Prevalence In Low-Income Children (2-18 Yr) Based On Participation In The Supplemental Nutrition Assistance Program: Nhanes 2003-2010, Matthew J. Landry

Honors Capstones

No abstract provided.

The Detection Of The Norovirus In A Wastewater Treatment Plant As A Potential Source Of Environmental Contamination, Dorothee Goettert

Honors Capstones

No abstract provided.

Development, Implementation, And Evaluation Of A Media-Awareness Wellness Program For High School Students, Rachel Firmin Johnson

Honors Capstones

No abstract provided.

Increased Dietary Docosahexaenoic Acid Consumption And Its Effect On Macular Pigment Optical Density: The Eye Health In College-Age Students Iii Pilot Study, Markita M. Lewis

Honors Capstones

No abstract provided.

The Ability Of Households In Southeast Louisiana Eligible For The Supplemental Nutrition Assistance Program To Meet The Food Recommendations Of The 2010 Dietary Guidelines For Americans, Alicia Rodriguez

Honors Capstones

No abstract provided.

Effects Of Dietary Fat On Resistant Starch Fermentation In Mice, Diana Badkoobeh

Honors Capstones

No abstract provided.