Food Science Commons^™

Characteristics Of Yogurt And Culture Bacteria As Affected By Ingredients That Help Treat Leaky Gut, Ricardo Jose Santos Aleman

LSU Doctoral Dissertations

At least 100 million people have leaky gut syndrome. This syndrome is associated with multiple health problems such as diabetes, celiac disease, asthma, multiple sclerosis, and inflammatory bowel disease, costing more than $3 billion per year to treat. L-Glutamine, quercetin, slippery elm bark, marshmallow root, N-acetyl-d-glucosamine (NAG), licorice root, maitake mushrooms, and zinc orotate have been reported to help treat leaky gut.

The objectives were to explore the characteristics of yogurt and culture bacteria as affected by ingredients that help treat leaky gut. In yogurt, the pH, titratable acidity, syneresis, viscosity, color, Streptococcus thermophilus, Lactobacillus bulgaricus, yeast and mold and …

Effect Of Uv-C Light Against Listeria Monocytogenes On Hydroponically Grown Lettuce And Its Effect On Quality, Ivannova Lituma

LSU Master's Theses

Controlled agriculture environments like hydroponic systems are a growing industry. However, studies have shown potential of microbial growth in hydroponic systems, highlighting the need for decontamination techniques to prevent the risk of foodborne diseases. This study evaluated the effect of UV-C light against Listeria monocytogenes on hydroponically grown lettuce and seeds. Lettuce leaves were spot inoculated (100μL) with Listeria monocytogenes cocktail and exposed to UV-C light (0.28mW/cm2) for 10 to 50 seconds in 10-second increments and 1 to 5 minutes in 1- minute increments. UV treated leaves were stored at 4C° and visually inspected for 5 days. Additionally, the smooth …

Effect Of Type Of Mulch And Biological Soil Amendments Of Animal Origin (Bsaao) On Microbial Food Safety Risk On Cucumbers And Carrots Irrigated With Contaminated Or Chlorinated Water, Juan Fernando Moreira Calix

LSU Doctoral Dissertations

A comprehensive understanding of the effect biological soil amendments of animal origin and physical soil amendments have on produce contamination is important for developing strategies to mitigate this risk. Our studies investigated the persistence of generic E. coli, E. coli O157:H7 and Salmonella spp. in soil and produce surfaces with different BSAAO and mulch applications, as well as the effectiveness of chlorine treatments in reducing enteric bacteria in the field. Cucumbers harvested from plots with paper mulch had the highest levels of E. coli (3.76 Log CFU/cm²) while samples from maize mulch had the lowest levels (1.68 …

Evaluating Sensory Quality, Willingness To Try, Risk And Product Appropriateness Of Seafood Byproducts, Silvia Jessenia Murillo Miguez

LSU Master's Theses

Valorization of food wastes/byproducts has become a major subject of research to increase sustainability of the global food supply chain. Seafood industries generate a substantial quantity of byproducts. An online survey was used to evaluate Willingness-to-try (WTT), the effect of a Consumer Safety Statement (CSS) on WTT, perceived risks and product appropriateness associated with seafood byproducts in food as influenced by gender and race. Based on the finding from this survey, a clean label catfish bone powder (CBP) was developed and used for breaded catfish sticks which were evaluated for sensory liking, emotional profile, and purchase intent. Previous consumers of …

Influence Of Hesperidin On The Physico-Chemical, Microbiological, And Sensory Characteristics Of Frozen Yogurt, Roberto Antonio Cedillos Hernández

LSU Master's Theses

Frozen yogurts contain yogurt culture bacteria which might impart health benefits to its consumers. Global frozen yogurt market sales are expected to grow 4.8% by 2028 which represents an important opportunity for the industry, consumers, and researchers. Polyphenols are metabolites found in plants which have antioxidant and anti-inflammatory properties and might prevent chronical diseases such as cancer, diabetes, and cardiovascular diseases. The objective of this study was to elucidate the effect of the polyphenol hesperidin on the physico-chemical, microbiological, and sensory characteristics of frozen yogurts. Hesperidin was incorporated in frozen yogurt at three concentrations (500, 250 and 125 mg/90g of …

Effects Of Sensory Cues On Product Acceptability And Consumer Perceptions, Emotions, And Behavior, Cristhiam Gurdian Curran

LSU Doctoral Dissertations

Visual and cognitive cues can affect overall liking (OL) and consumer perceptions, emotions, and behavior. The first study explored the effect of product color difference on the liking, perception, and purchase intent (PI) of cheese-flavored-tortilla chips (CFTC) formulations (A and B) on serving plates (plastic, foam, and paper). Color differences between formulations influenced crunchiness and saltiness liking and perception, which together with overall flavor liking and formulation, mainly determined CFTC OL. Although having similar fracturability (N) and sodium content, formulation A had higher crunchiness and saltiness likings. PI was influenced by crunchiness, saltiness, and OF liking with 37, 49, and …

Development Of A Non-Dairy Yogurt Alternative Using High Protein Brown And White Rice Flour, Anita Morris

LSU Doctoral Dissertations

There is a great demand for plant-based yogurt alternatives (YAs) due to societal prevalence of milk sensitivities and allergies as well as some consumers abstaining from animal-derived products. Producing YAs from rice flour has considerable potential because (1) rice is not a major food allergen, (2) using rice flour is more economical compared to plant milks, the main ingredient in commercially available YAs, and (3) there are no rice-based yogurt alternatives in today’s marketplace. The overall goals of this study were to develop probiotic YAs from brown and white rice flours, and to evaluate their storage stability, probiotic’s survival in …

Producing Bacterial Cellulose From Coffee (Coffea Arabica) Kombucha Fermentation And Assessment Of Its Efficacy To Remove Pathogenic Bacteria From Contaminated Water And A Pollutant Dye From Aqueous Solution, Federico Bueno

LSU Doctoral Dissertations

Bacterial cellulose (BC), composed of cellulosic nanofibrils, is synthesized by a symbiotic culture of bacteria and yeast during the fermentation of coffee kombucha (CK). BC may be used as a purification agent and adsorbent. The overall goal of this project was to synthesize BC from CK fermentation and assess its efficacy in removing pathogenic bacteria from contaminated water and a pollutant dye from aqueous solution. The objectives of this study were to: (1) compare BCs that were produced from different carbohydrate substrates in the fermentation of CK, (2) characterize dried BCs produced using various drying methods, (3) evaluate BC as …

Development And Characterization Of Physicochemical Properties And Consumer Perception Of Gluten-Free Oat-Based Snack Bars Containing Orange Peel, Andrea R. Muela Negrete

LSU Master's Theses

Orange peel (OP) is a major byproduct from orange juice production, and is a good source of fiber and flavonoids, particularly nobiletin. Nobiletin has been reported to exhibit pharmacological activities including anti-inflammation and antioxidation. Incorporating orange peel into food products would harness economic benefit of this industrial byproduct and support a sustainable food chain. This study was performed to evaluate consumer perception of gluten-free oat-based snack bars (GFOSBs) containing orange peel.

In the first study three GFOSBs were formulated to contain 17%, 20%, and 23% orange peel by weight. Consumers (N=103) evaluated GFOSBs for sensory liking (9-point hedonic scale) and …

Evaluating Consumers’ Perception Of Gluten-Free/Clean-Label Muffins, Vanesa Chicaiza

LSU Master's Theses

The gluten-free-natural-ingredient (GF/NCL) product demand is globally increasing and driven by the growing prevalence of celiac-diseases. Nevertheless, the quality of GF product is often inferior compared to wheat-flour counterparts due to the removing of gluten, which, compromises the appearance, structure, and nutritional value. The addition of fiber into GF/NCL products has been considered to improve nutritional values while maintaining quality attributes. According to the US-FDA, a product containing ≥2.5 grams of fiber/serving can be claimed as a “good-source-of-fiber.” However, adding dietary fiber to baked products affects their qualities, and hence possibly lowering product acceptability.

The main objective of this research …

Fish Gelatin Coating As An Antimicrobial Agent On Wild-Caught Louisiana Shrimp, Hunter S. Songy

LSU Master's Theses

Seafood has a short shelf-life because of its unstable nature. Shrimp has a shelf-life of only eight days. Edible coatings, like those made from gelatin, have been used to apply antimicrobial agents to food product surfaces. Organic acids are naturally occurring, low-cost options that have been proven to effectively reduce bacterial loads on food products and extend their shelf-life. Gelatin can be extracted from catfish skin, helping to reduce product waste. The purpose of this study was to determine the potential effectiveness of fish skin gelatin as an antimicrobial coating alone and in combination with lactic acid, and then compare …

Evaluation Of The Effectiveness Of Aeration During Washing To Dislodge Microorganisms From Cucumbers And Bell Peppers, Julysa Benitez

LSU Master's Theses

Microorganisms may attach firmly to produce matrices and could affect the efficacy of disinfectants. The purpose of this study is to evaluate the effectiveness of aeration to reduce microbial risk during washing and sanitizing of fresh produce. Bell peppers and cucumbers were spot inoculated with Listeria innocua, E. coli O157:H7, and Salmonella; after drying in the biosafety cabinet for 1h, these were washed with water or sanitized with 100 ppm of chlorine for 3 min with or without aeration. Bell pepper and cucumber samples were placed in sterile bags with PBS after treatment. Bell peppers were processed in the stomacher …

Consumers' Perception Of Food Containing Insect Protein Affected By Serving Condition And Questionnaire Protocol, Yupeng Gao

LSU Master's Theses

Edible insects are a new sustainable protein source compared to meat. Western consumers show aversion to the prospect of insects as food due to unfamiliarity, food neophobia, and negative emotions. This dissertation research explored consumers’ emotional perception of insect-based food products presented under different testing conditions and examined the influence of other people’s emotional expressions towards consumers’ purchase decisions. To identify and rate emotion intensities, tests were conducted using three presenting formats including text, image, and an actual product; all of which were applied for both cookies containing insects and without insects. In addition to these presenting formats, news of …

High Protein Rice Flour In The Development Of Gluten Free Pasta, Ana Isabel Serrano Marana

LSU Master's Theses

Cahokia Rice is a biofortified high protein (10-12%) variety of rice. This rice has been evaluated and studied to create gluten-free products like cakes, muffins, and bread. This study was aimed to develop and analyze a gluten-free pasta using this high protein rice. Different pasta made with high-protein-white-rice-flour (HPWRP), high-protein-brown-rice flour (HPBRP), commercial-white-rice flour (CWRP), commercial-brown-rice flour (CBRP), and wheat-pasta (WP) as control were prepared and dried.

The cooking quality of the pasta was evaluated by measuring pasting properties, cooking time, water absorption capacity and cooking loss. Physical properties such as color and texture (firmness, compression energy, and work of …

Evaluation Of Food Safety Practices At Louisiana Summer Feeding Sites And Food Safety Communication Via Social Media During Crises, Peyton E. Haynes

LSU Master's Theses

The United States Department of Agriculture’s Summer Food Service Program (SFSP) provided children under the age of 18 in low-income areas 145 million free meals in 2018 through summer feeding sites. Food safety issues arise for Louisiana feeding sites due to time-temperature controlled foods served, weather patterns in Louisiana, seasonal staff and short operation time, and lack of equipment. The purpose of this study was to assess food safety practices at Louisiana feeding sites through observational study with intention to compare food practices at institutional and noninstitutional sites. A rubric following USDA food safety guidelines based on categories: hot holding, …

Development Of Pudding And Gravy With Rice Starch, Stearic Acid And Lysine, Andrea Suazo Rosales

LSU Master's Theses

Starch is a common ingredient used in the food industry for many products. This study was focused on developing products (gravy and pudding) with a clean label starch. The clean label starch was prepared with rice starch, 1% stearic acid, and 6% lysine, which was gelatinized, freeze-dried, and grinded. Both products were made with three different starch types: rice starch, commercial modified starch, and clean label starch. These starches were analyzed with DSC, SEM, Megazyme Amylose/Amylopectin kit, and RVA. With the results from the RVA, SEM, and DCS it is inferred that the freeze-drying process has altered the structure of …

Effect Of Contact Time And Attachment Level Of Bacterial Pathogens On Efficacy Of Chlorine Treatment In Fresh Produce, Jyoti Aryal

LSU Master's Theses

Bacterial attachment or biofilm formation on produce surfaces may result in limited penetration ability of sanitizers. This study examined the wet and dry contact time (WCT or DCT) of produce during washing with sanitizer and evaluated the effect of attachment level of Listeria monocytogenes during storage on the efficacy of chlorine treatment. Spinach and bell peppers were inoculated with Listeria monocytogenes and Salmonella enterica. The produce was then washed by dipping into a 100 ppm of chlorine solution for 1 min and an additional 1, 3, or 5 minutes (WCT) or removed from the chlorine solution and held for 1, …

Development Of High-Fiber Belgian Waffles And Evaluation Of Product Acceptance, Product-Elicited Emotion, And Purchase Intent By Millennial Consumers, 2253299685 Velasquez

LSU Master's Theses

Dietary fiber may help reduce LDL cholesterol levels and prevent cardiovascular diseases. The US-FDA recommends 14g of fiber/1000kcal consumed, but Americans consume/day. A product containing ≥5 grams of fiber/serving can be claimed as a high-fiber product. Adding dietary fiber to food affects their physicochemical properties and sensory quality, possibly leading to less product acceptability. This study was performed to assess selected physicochemical properties and consumer perception of “high-fiber” Belgian waffles. Waffles were formulated to have fiber contents of

Influence Of Health Beneficial Monk Fruit Sweetener On Microbial And Physicochemical Characteristics Of Camel Milk Yogurt, Alina Buchilina

LSU Master's Theses

Camel milk contains all the essential nutrients found in cow’s milk and has potentially health beneficial compounds such as anti-carcinogenic, anti-hypertensive, anti-inflammatory, and insulin and insulin-like substances. Camel milk has been reported as a treatment for diseases such as diabetes and autism. Camel milk favorably alters gut microbiota to a higher abundance of bifidobacteria.

Sweetness is one of the most desirable tastes in people’s diet; however, the surplus consumption of sugar has a negative effect on human health. Monk fruit sweetener is a natural zero caloric sweetener with many health beneficial properties, namely, prevention of asthma, prevention of oxidation, liver …

Sodium Propionate And Sodium Butyrate Promote Fatty Acid Oxidation In Hepg2 Cells Under Oxidative Stress, Kristina Jazmin Cook

LSU Doctoral Dissertations

Non-alcoholic fatty liver disease (NAFLD) is a spectrum of disturbances that includes simple steatosis, non-alcoholic steatohepatitis (NASH), fibrosis, cirrhosis, and liver cancer. NAFLD affects individuals that consume the typical Western diet consisting of high levels of fats and carbohydrates. The increase in circulating free fatty acids, palmitate and oleate, or lipopolysaccharides (LPS), induce oxidative stress and pro-inflammatory cytokine production in the liver, which all contribute to NAFLD progression. In this study, we are evaluating the mRNA expression of genes associated with fatty acid oxidation (FAO) and the protein expression of pro-inflammatory cytokines related to NAFLD using the HepG2 human liver …

Effect Of Storage Temperature On The Survival Or Growth Of Listeria Monocytogenes On Whole And Fresh-Cut Produce, Juan F. Moreira Calix

LSU Master's Theses

Whole and fresh-cut produce are minimally processed and, therefore susceptible to microbial contamination. This study examined the survival or growth of Listeria monocytogenes on whole, and fresh-cut produce at different storage temperatures. Fresh fruits (cantaloupes, pears, pineapples, papayas, and watermelon) and vegetables (broccoli, cauliflower, lettuce, kale, and green bell peppers) were cut into 25 g pieces and were spot inoculated with 0.5 mL (8 Log CFU/mL) of Listeria monocytogenes. Inoculated fresh-cut samples were stored at 4°C or 13°C for 6 days. To represent the outer surface of the produce, cantaloupes and green bell pepper disks (20 cm2) were cut with …

Prevalence And Control Of Salmonella Spp. And Sanitary Indicator Microorganisms In Wild Caught And Farm Raised Catfish (Ictalurus Punctatus), Bilan Costley Jessie

LSU Doctoral Dissertations

Catfish inspections have transitioned from the U.S. Food and Drug Administration (FDA) to the United States Department of Agriculture (USDA). The USDA Food Safety and Inspection Service (FSIS) and Food Emergency Response Network (FERN) consider it important to assess the food safety risk associated with consuming catfish in the United States. Surveillance of farm-raised and wild-caught catfish for pathogens is relevant as a public safety protocol. The purpose of this study was to detect the prevalence of Salmonella spp., identify and examine isolates for antibiotic resistance to clinical treatments, evaluate the effectiveness of antimicrobial chemical treatments against Salmonella enterica, and …

Occurrence Of Aflatoxin And Zearalenone In Concentrate Feeds And Milk Contamination With Aflatoxin M1 And Bacterial Pathogens In Malawi, Chunala Alexico Njombwa

LSU Doctoral Dissertations

Multiple studies were conducted in Malawi to: 1) evaluate knowledge and perception of dairy farmers towards molds, mycotoxins and associated adverse effects; 2) evaluate dairy farmers’ knowledge and perception on milk contamination, proper milk handling practices and adverse health effects associated with raw milk consumption; 3) determine levels of aflatoxins and zearalenone (ZEN) in concentrate feedstuffs; 4) assess prevalence and levels of aflatoxin M₁(AFM₁) in raw milk, dietary exposure and estimate HCC risks to children and adults; 5) assess Salmonella and E. colicontamination in raw and processed milk from selected small scale farms and major …

Comparison Of The Kinetic Parameters Of Escherichia Coli 0157:H7, Listeria Monocytogenes And Salmonella Typhimurium Derived From The Baranyi And Huang Models In A Chemically Defined Minimal Medium, Jose Isidro Fuentes

LSU Master's Theses

Microbial growth can be characterized by parameters such as lag time, growth rate, and maximum population density at any specific point of time. Mathematical models that predict microbial growth of foodborne pathogens are increasingly used in the food industry as a viable alternative to traditional methods of microbial enumeration. The Baranyi model has been widely used as the primary model of choice by many authors because of its performance and accuracy. The most recently developed Huang model has been less implemented and few comparisons between the Baranyi and Huang models have been made when modeling pathogenic growth. For this research, …

In Vitro And In Vivo (Mouse Model) Investigation Of The Efficacy Of Probiotic Bacteria Lactobacillus Casei And Lactobacillus Rhamnosus Incorporated In Tomato Juice, Bennett Atta Dzandu

LSU Doctoral Dissertations

Fruit and vegetable production in Ghana were estimated at 371,811 tons in 2017 with tomatoes ranked among the top four most cultivated vegetables. The majority of the production goes to waste due to spoilage and lack of processing. Probiotics have recently gained much attention owing to claims about their potential health benefits. However, the inability of certain probiotics to survive gastric conditions remain a potential setback. Tomatoes can be processed into value added products including those fortified with probiotics. Such value-added products can be used as protectant for the delivery of probiotics to improve gut health. The objective of this …

Sodium Reduction Through Salt Substitute Mixtures, Visual Cues, And Salt Level Statements, And Its Effects On Consumer Perception: A Case Of Barbecue Sauce, Jose Ramon Alonso Marenco

LSU Doctoral Dissertations

Cardiovascular diseases are the leading causes of death in the USA and associated with excessive sodium (Na) intake. Thus, reducing the Na intake is vital to alleviate health concerns. One serving of the average Barbecue sauce (BBQs) may contribute around 15%-20% of the daily Na allowance (2,300 mg). No research has evaluated salt substitutes or visual cues on salty taste perception of BBQs, thus it was the aim of this research. Consumer studies followed a Balanced Incomplete Block (BIB) designs. For Study I, BBQs prototypes followed a 3-components (NaCl : KCl : Glycine) mixture design, with 38% - 87% less …

Antimicrobial Properties Of Different Chitosans Against Gram-Negative And Gram-Positive Foodborne Pathogens In Food Products, Nancy Katherine Rubio Zapata

LSU Doctoral Dissertations

Chitosan is a polymer derived of the deacetylation of chitin that is one of the most abundant material in nature. Chitosan solutions applied as edible coatings, and dipping solutions have shown positive results in the extension of shelf life on seafood and meat products. The purpose of this study was to evaluate the antimicrobial activities of a newly invented high molecular weight water-soluble (HMWWS) chitosan against selected Gram-negative (Salmonella Typhimurium, Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae) and Gram-positive (Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus) foodborne pathogens (initial inoculation of 6.5 Log). Chitosans with …

Improving The Food Safety Of Louisiana Strawberry Industry, Jose L. Brandao Delgado

LSU Doctoral Dissertations

Escherichia coli O157:H7, Salmonella sp., and Listeria monocytogenes have been linked to foodborne outbreaks in produce. The most recent outbreaks in produce have been associated with irrigation water due to infiltration of well water or water run-off from contaminated sources. The Food Safety Modernization Act (FSMA) requires all irrigation water to be safe for use on produce, as a strategy to reduce foodborne illnesses. A surfactant modified zeolite (SMZ) filtration system could provide produce farmers with a sustainable low-cost system for high-quality and safe irrigation water. The objective of this study was to evaluate the effectiveness of hexadecyltrimethylammonium …

Ale Beer Containing Free And Immobilized Lactobacillus Brevis, A Potential Delivery System For Probiotics, Kriza Faye Albacite Calumba

LSU Master's Theses

Probiotic products can improve gastrointestinal health and probiotics in ale beer may be attractive to health-conscious consumers. However, the alcohol in beer may decrease probiotic viability. A powder produced from durian fruit (Durio zibethinus) rind, a by-product that is currently unutilized, contains large amounts of fiber which can be used to immobilize probiotics. The objective of this study was to produce ale beer with free Lactobacillus brevis (FLB) and immobilized cells (ILB) on durian rind powder (DRP) as a potential delivery system for probiotics. L. brevis was immobilized in DRP then the morphology of FLB and ILB was …

Efficacy Of Aqueous And Gaseous Chlorine Dioxide In Reducing Salmonella, E. Coli O157:H7, And Listeria Monocytogenes On Sweet Potatoes, Strawberries, And Blueberries, Phillip Luu

LSU Master's Theses

Chlorine dioxide, a stronger oxidizing agent, is effective against a broad spectrum of microorganisms. This study evaluated the efficacy of chlorine dioxide on reducing Salmonella, E. coli O157:H7, and Listeria monocytogenes on sweet potato (aqueous ClO₂), strawberries and blueberries (gaseous ClO₂). Sweet potatoes inoculated with multi-strain cocktails of each bacterial pathogen (10⁷ CFU/cm²) were treated with 5 ppm aqueous ClO₂ or water for 10, 20, and 30 minutes. Strawberries and blueberries spot inoculated with multi-strain cocktails of each bacterial pathogen (10⁷ CFU/g) were treated with gaseous ClO₂ sachets (63 mg/l for strawberry and 30 mg/l for blueberry) …