Entomology Commons^™

Bioinformatics Analysis Of The Microsporidia Sp. Mb Genome: A Malaria Transmission-Blocking Symbiont Of The Anopheles Arabiensis Mosquito, Lilian Mbaisi Ang’Ang’O, Jeremy Keith Herren, Özlem Tastan Bishop

All Peer-Reviewed Publications

Background: The use of microsporidia as a disease-transmission-blocking tool has garnered significant attention. Microsporidia sp. MB, known for its ability to block malaria development in mosquitoes, is an optimal candidate for supplementing malaria vector control methods. This symbiont, found in Anopheles mosquitoes, can be transmitted both vertically and horizontally with minimal effects on its mosquito host. Its genome, recently sequenced from An. arabiensis, comprises a compact 5.9 Mbp. Results: Here, we analyze the Microsporidia sp. MB genome, highlighting its major genomic features, gene content, and protein function. The genome contains 2247 genes, predominantly encoding enzymes. Unlike other members of the …

Unearthing Lactococcus Lactis And Scheffersomyeces Symbionts From Edible Wood-Boring Beetle Larvae As A Bio-Resource For Industrial Applications, Shadrack Kibet, Cynthia M. Mudalungu, Njogu M. Kimani, John Mark O. Makwatta, James Kabii, Subramanian Sevgan, Segenet Kelemu, Chrysantus M. Tanga

All Peer-Reviewed Publications

Background: Gut microbiota have several advantages in influencing the host nutrition, metabolism, immunity and growth. However, the understanding of the gut microbiota in key edible wood-boring beetle larvae remain largely undefined. In the present study, the characteristics of the gut microbiota of two edible wood-boring species (Titocerus jaspideus and Passalus punctiger) from two indigenous forested areas were investigated. Results: Over 50% of Amplicon Sequence Variants (ASVs) constituted of Firmicutes in T. jaspideus. The dominant phyla in both beetle species were Bacteroidota (4.20–19.79%) and Proteobacteria (15.10–23.90%). Lactococcus lactis was the most abundant and core prokaryote in the guts of T. jaspideus. …

Shaping The Microbial Landscape: Parasitoid-Driven Modifications Of Bactrocera Dorsalis Microbiota, Rehemah Gwokyalya, Jeremy K. Herren, Christopher W. Weldon, Shepard Ndlela, Joseph Gichuhi, Nehemiah Ongeso, Anne W. Wairimu, Sunday Ekesi, Samira A. Mohamed

All Peer-Reviewed Publications

Koinobiont endoparasitoids regulate the physiology of their hosts through altering host immuno-metabolic responses, processes which function in tandem to shape the composition of the microbiota of these hosts. Here, we employed 16S rRNA and ITS amplicon sequencing to investigate whether parasitization by the parasitoid wasps, Diachasmimorpha longicaudata (Ashmaed) (Hymenoptera: Braconidae) and Psyttalia cosyrae (Wilkinson) (Hymenoptera: Braconidae), induces gut dysbiosis and differentially alter the gut microbial (bacteria and fungi) communities of an important horticultural pest, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae). We further investigated the composition of bacterial communities of adult D. longicaudata and P. cosyrae to ascertain whether the adult parasitoids …

A Mathematical Model For Mapping The Insecticide Resistance Trend In The Anopheles Gambiae Mosquito Population Under Climate Variability In Africa, Komi Mensah Agboka, Mark Wamalwa, James Mutuku Mutunga, Henri E.Z. Tonnang

All Peer-Reviewed Publications

The control of arthropod disease vectors using chemical insecticides is vital in combating malaria, however the increasing insecticide resistance (IR) poses a challenge. Furthermore, climate variability affects mosquito population dynamics and subsequently IR propagation. We present a mathematical model to decipher the relationship between IR in Anopheles gambiae populations and climate variability. By adapting the susceptible-infected-resistant (SIR) framework and integrating temperature and rainfall data, our model examines the connection between mosquito dynamics, IR, and climate. Model validation using field data achieved 92% accuracy, and the sensitivity of model parameters on the transmission potential of IR was elucidated (e.g. μPRCC = …

First Records Of The Spider Wasp Entypus Ochrocerus Dahlbom (Hymenoptera: Pompilidae: Pepsinae) From The Continental United States, Jamaica, And The Bahamas, Frank E. Kurczewski, Rick C. West, Dionne Newell, Scott Johnson

Insecta Mundi

A small population of Entypus ochrocerus Dahlbom, a predominantly Greater Antilles spider wasp (Hymenoptera: Pompilidae: Pepsinae), is reported for the first time from the continental United States based on photographs and associated information from iNaturalist.org. The species was initially misidentified online as E. unifasciatus unifasciatus (Say) or Entypus sp. However, Entypus u. unifasciatus individuals from east of the 100th Meridian in the United States typically have melanochromatic (dark) wings with a subapical xanthochromatic (orange-amber) patch. Entypus ochrocerus from Miami-Dade County, Florida is differentiated from E. u. unifasciatus by its black body with dark greenish blue reflections …

First Record Of The Mexican-M Hairstreak Parrhasius Moctezuma (Clench, 1971) (Lepidoptera: Lycaenidae) In Texas, Usa, And Possible Sighting Of The Tequila Giant Skipper Aegiale Hesperiaris (Walker, 1856) (Lepidoptera: Hesperiidae), Sajan Kc, Anisha Sapkota

Insecta Mundi

We report two significant butterfly sightings in the United States, observed during an opportunistic photographic survey conducted in south Texas between December 2022 and January 2023. Parrhasius moctezuma (Clench, 1971) (Lepidoptera: Lycaenidae) was sighted at the National Butterfly Center in Mission, Texas on January 5, 2023, marking the first documented record of the species in Texas and only the second in the United States, as confirmed by photographic evidence. Additionally, Aegiale hesperiaris (Walker, 1856) (Lepidoptera: Hesperiidae) was potentially observed at the same location on December 4, 2022, although lacking photographic documentation or a collected specimen, highlighting the need for further …

The Puzzling Distribution Of Platphalonidia Aenigmana, New Species, From The Western U. S. A. And Colombia (Lepidoptera: Tortricidae: Cochylina), John W. Brown

Insecta Mundi

Platphalonidia aenigmana Brown, new species (Lepidoptera: Tortricidae), is described and illustrated from Arizona, California, and Oregon, U. S. A. and Colombia, South America. The new species has been reared on numerous occasions from Garrya elliptica Dougl. ex Lindl. (Garryaceae) in northern California and Oregon, once on Arctostaphylos sp. (Ericaceae) in southern California, and once on Gomphrena globosa L. (Amaranthaceae) in Colombia. Various hypotheses are explored to explain the unusual disjunct geographic distribution of the species. In addition, Phalonidia bassii Razowski (Tortricidae) is transferred to Saphenista Walsingham, resulting in Saphenista bassii (Razowski, 1999), new combination.

A New Species Of Cymatodera Gray (Coleoptera: Cleridae) From Central Arizona, Jacques Rifkind

Insecta Mundi

Cymatodera campbelli, new species (Coleoptera: Cleridae), is described from central Arizona, United States. Though superficially similar to several other small species of Southwestern Cymatodera Gray that are commonly collected at lights, this apparently rare species has distinctive coloration and surface sculpturing. Its discovery suggests that systematic collecting even in areas that have historically attracted entomological attention may still yield additions to our fauna.

New Country Records For Macroscython Granulosus Otto, 2016 (Coleoptera: Eucnemidae: Dromaeolinae: Dromaeolini), With Redescription Of The Female, Robert L. Otto, Jiří Háva

Insecta Mundi

New country records for Macroscython granulosus Otto, 2016 (Coleoptera: Eucnemidae: Dromaeolinae: Dromaeolini), a eucnemid known only from Laos and Thailand, are reported from China and Nepal. A redescription and images of the female are provided.

Genomic Analysis Reveals Hidden Species Diversity In Emesis Fabricius (Lepidoptera: Riodinidae), Jing Zhang, Qian Cong, Jinhui Shen, Leina Song, Nick V. Grishin

Insecta Mundi

Genomic analysis of Emesis [Fabricius], 1807 (Lepidoptera: Riodinidae Grote, 1895) reveals species richness higher than anticipated. As a result, one subgenus, 22 species, and one subspecies are proposed as new (type species or type localities in parenthesis): Diogenia Grishin, new subgenus (Emesis diogenia Prittwitz, 1865), Emesis (Emesis) aerunda Grishin, new species (Peru: Rio Pachitea, Monte Alegre), Emesis (Emesis) bartica Grishin, new species (Guyana: Cuyuni-Mazaruni), Emesis (Emesis) fatimellina Grishin, new species (Brazil: Santa Catarina), Emesis (Emesis) panamella Grishin, new species (Panama: Darién), Emesis (Mandania) mandora Grishin, new species (Ecuador: …

Review Of Nealyda Dietz (Lepidoptera: Gelechiidae: Apatetrinae) With Description Of A New Species From The Florida Keys, Sidney V. Bennett, James E. Hayden

Insecta Mundi

Nealyda grandipinella Bennett and Hayden, new species, is described from Big Pine Key, Florida, USA. Photographs of the adult and of male and female genitalia are provided for N. grandipinella and its congeners within the state of Florida. Nealyda Dietz (Lepidoptera: Gelechiidae) is provisionally transferred from Anomologinae to Apatetrinae based on characters of the genitalia. The homology and terminology of the processes arising from the vinculum of gelechiid genitalia are explored. The potential host plant of the new species is discussed, along with other undescribed species of Nealyda.

First Record Of The Camphor Shot Borer, Cnestus Mutilatus (Blandford) (Coleoptera: Curculionidae: Scolytinae), In Massachusetts, Lawrence E. Barringer

Insecta Mundi

Cnestus mutilatus (Blandford) (Coleoptera: Curculionidae: Scolytinae) is reported from Massachusetts, United States for the first time. Brief trapping information is provided.

Red Harvester Ants Pogonomyrmex Barbatus (Smith, 1858) (Hymenoptera: Formicidae) Do Not Distinguish Between Sorghum Head Mold Symptomatic And Asymptomatic Seeds, Lilly Victoria Elliott-Vidaurri, Hannah J. Penn, Robin A. Choudhury

School of Earth, Environmental, & Marine Sciences Faculty Publications

Red harvester ants, Pogonomyrmex barbatus (Smith, 1858) (Hymenoptera: Formicidae), common in the Lower Rio Grande Valley of Texas, are known to gather seeds from areas around their nests and store the seeds inside their nests for later consumption. As these ants often nest in and near agricultural fields, some of these seeds may be from crops and may also be infected with fungal plant pathogens. These pathogens can degrade seed coats and may cause the seeds to rot within the ant nests, decreasing storage time and potentially spreading the pathogen to other stored seeds. We studied how head mold, a …

New Taxa Of Butterflies Supported By Genomic Analysis, Jing Zhang, Qian Cong, Jinhui Shen, Leina Song, Nick V. Grishin

The Taxonomic Report of the International Lepidoptera Survey

Continuing with the genomic analysis of butterflies, we present a taxonomic update. As a result of this work, 3 genera, 6 subgenera, 16 species, and 2 subspecies are described as new. New genera and subgenera are (type species in parenthesis): Systasia Grishin, gen. n. (Hesperia zampa W. H. Edwards, 1876) and Asysta Grishin, gen. n. (Systasea microsticta Dyar, 1923) in Pyrgini Burmeister, 1878 and Alyco Grishin, gen. n. (Styriodes quota Evans, 1955) in Moncina A. Warren, 2008; Ocynthus Grishin, subgen. n. (Ocybadistes hypomeloma Lower, 1911) of Potanthus Scudder, 1872, Arrhona Grishin, subgen. n. (Padraona tranquilla …

Integrating Ecophysiology And Omics To Unlock Crop Response To Drought And Herbivory Stress, Insha Shafi, Manish Gautam, Rupesh Kariyat

Entomology Faculty Publications and Presentations

Introduction: Plants are constantly exposed to a diverse spectrum of environmental stressors that typically occur concurrently as multifactorial challenges, both spatially and temporally (Joshi et al., 2024). These stressors include abiotic stresses, such as drought, flooding, salinity, nutrient deficiencies, metal toxicity and extreme temperatures, as well as biotic pressures such as herbivory, pathogen attacks, and inter and intraspecific competition (Porter et al., 2020; González Guzmán et al., 2022). Drought and herbivory are arguably among the most critical factors that limit crop productivity in agricultural settings (Gautam et al., 2024), making it imperative to understand plant responses to their combined effects. …

Notes On North American Butterflies, 2, Harry Pavulaan, Janice E. Arndt

The Taxonomic Report of the International Lepidoptera Survey

New natural history elements and distribution records of several North American butterflies are reported. While diversity and distribution of butterflies in North America are commonly believed to be fully known, the findings presented here show that much is yet to be learned of our butterfly fauna.

Nebline, November/December 2024

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Positive Insights Shared Regarding CHIME: Head Start University Partnership Project, Jaci Fogel

Diabetes Awareness: Small Changes, Big Impact, Kayla Colgrove

Recipe of the Month: Turkey Cranberry Meatballs, Kayla Colgrove

Commercial/ Noncommercial Pesticide License

Love Gardening? Become a Master Gardener!

Garden Guide: Things to Do This Month

Upcoming Green Industry Conferences

Managing the Demands of Water & Wastewater Systems Can Make Gatherings More Enjoyable, Becky Schauerman

It’s Time to Plant Fall Cover Crops, John Nelson

A Quick Guide to Fleas, Kait Chapman

Heart of 4-H Award: Shane Cuttlers and Dena Noe

4-H Specialty Clubs Invite New Members

Nebraska 4-H Fed Steer …

Lupin Root Diseases - Diagnosis And Management, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Root, hypocotyl and stem base diseases can be significant problems in lupin crops, reducing stand density, plant vigour and yield. Root disease is present in nearly all narrow leafed lupin (Lupinus angustifolius) paddocks but in the majority of paddocks has only a small impact on crop development. However, in paddocks where high levels of root rot occurs, plant establishment, seedling vigour and crop yield can be significantly affected.

When a root disease is present in a crop very little can be done to manage it in that cropping season. It is therefore vital that the cause is correctly identified to …

Chronologically Inappropriate Morphogenesis (Chinmo) Is Required For Maintenance Of Larval Stages Of Fall Armyworm, Xien Chen, Jinmo Koo, Surjeet Kumar Arya, Subba R. Palli

Entomology Faculty Publications

Broad complex (Br-C) and eip93F (E93) transcription factors promote insect metamorphosis from larva to pupa and from pupa to adult, respectively. Recently, chronologically inappropriate morphogenesis (Chinmo) has been proposed as a larval specifier in Drosophila melanogaster. However, whether Chinmo is required for larval maintenance in lepidopteran insects, the underlying mechanisms involved in maintaining the larval stage, and its interactions with the JH signaling pathway are not well understood. Here, we used a binary transgenic CRISPR/Cas9 system to knockout Chinmo and Kr-h1 (primary response gene in the JH signaling pathway) in the fall armyworm (FAW). Kr-h1 knockout induced premature metamorphosis only …

Repeated Shifts In Sociality Are Associated With Fine-Tuning Of Highly Conserved And Lineage-Specific Enhancers In A Socially Flexible Bee, Beryl M. Jones, Andrew E. Webb, Scott M. Geib, Sheina Sim, Rena M. Schweizer, Michael G. Branstetter, Jay D. Evans, Sarah D. Kocher

Entomology Faculty Publications

Comparative genomic studies of social insects suggest that changes in gene regulation are associated with evolutionary transitions in social behavior, but the activity of predicted regulatory regions has not been tested empirically. We used self-transcribing active regulatory region sequencing, a high-throughput enhancer discovery tool, to identify and measure the activity of enhancers in the socially variable sweat bee, Lasioglossum albipes. We identified over 36,000 enhancers in the L. albipes genome from 3 social and 3 solitary populations. Many enhancers were identified in only a subset of L. albipes populations, revealing rapid divergence in regulatory regions within this species. Population-specific enhancers …

European Wasp: Declared Pest, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity factsheets

The European wasp is one of the worst wasps in the world — harmful to people, outdoor lifestyles, and horticultural and agricultural industries. Early detection of wasp activity is critical to prevent the wasp establishing in Western Australia (WA). This is achieved via collaboration between residents, businesses, industry, and government.

European Wasp Information For Local Government, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity factsheets

The department’s European wasp surveillance and eradication program is a yearly campaign, aimed at detecting the presence of this destructive pest as early as possible so that nests can be found and eradicated.

The continued success of European wasp surveillance and eradication program relies on surveillance in metropolitan Perth and regional areas, strong community awareness, and participation between residents, business owners, community groups, industry, and government.

An Overview Of Tsetse Fly Repellents: Identification And Applications, Olabimpe Y. Orubuloye, Njelembo J. Mbewe, David P. Tchouassi, Abdullahi A. Yusuf, Christian W.W. Pirk, Baldwyn Torto

All Peer-Reviewed Publications

Tsetse flies are vectors of the parasite trypanosoma that cause the neglected tropical diseases human and animal African trypanosomosis. Semiochemicals play important roles in the biology and ecology of tsetse flies. Previous reviews have focused on olfactory-based attractants of tsetse flies. Here, we present an overview of the identification of repellents and their development into control tools for tsetse flies. Both natural and synthetic repellents have been successfully tested in laboratory and field assays against specific tsetse fly species. Thus, these repellents presented as innovative mobile tools offer opportunities for their use in integrated disease management strategies.

Ipydisp V2 Alias Dudutracker: A Web-Based Version, Komi Mensah Agboka, Elfatih M. Abdel-Rahman, Samira A. Mohamed, Sunday Ekesi

All Peer-Reviewed Publications

This study presents the updated version v2 of IpyDisp named DuduTracker which improves on the window-only-requirement of IpyDisp. The updated version is web-based that can be used in alternative operating systems like Ubuntu, Mac, Linux, and others. The update's effectiveness was also evaluated using a survey involving a diverse range of users including students, data analysts, and academic researchers from different age groups, geographical locations, and computer literacy levels. Areas for future enhancement were identified, primarily focused on making the software responsive to various screen types and improving certain interface aspects.

Tritrophic Interactions Mediated By Zoophytophagous Predator-Induced Host Plant Volatiles, Bashiru Adams, Abdullahi Ahmed Yusuf, Baldwyn Torto, Fathiya Mbarak Khamis

All Peer-Reviewed Publications

The zoophytophagous mirid predator Nesidiocoris tenuis and the ectoparasitoid Stenomesius japonicus are important biological control agents for several agricultural pests including the invasive leafminer, Phthorimaea absoluta, a destructive pest of Solanaceous crops especially tomato in sub-Saharan Africa. However, little is known about how feeding by N. tenuis can influence the tritrophic interactions in the tomato plant. Here, we tested the hypothesis that N. tenuis phytophagy would influence the tritrophic olfactory interactions between the host plant tomato and pest, predator, and parasitoid. In olfactometer assays, P. absoluta females and N. tenuis adults were both attracted to constitutive volatiles released by the …

Determining The Impact Of Lethal Temperature And Relative Humidity When Combined With Various Insecticide Classes Against A Bed Bug (Cimex Lectularius) Population., Bandana Shrestha

LSU Master's Theses

Common bed bugs (Cimex lectularius) are obligatory blood-feeding pests that have adapted well to survive in challenging environmental conditions. Known for their high tolerance to dehydration, low water loss, and high critical thermal maximum, they have become difficult to manage. Environmental factors, particularly temperature and humidity, play a significant role in their survival. However, with growing resistance to insecticides, integrated pest management (IPM) strategies are now essential for effective bed bug control. A key strategy in IPM involves combining insecticides with heat treatments. This method aims to overcome the limitations of heat treatment alone, as bed bugs may …

Yellow Crazy Ant, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity factsheets

Yellow crazy ants (Anoplolepis gracilipes) are believed to be native to west Africa or Asia. They are not present in Western Australia (WA) and are prohibited.

Yellow crazy ants were first observed in Australia in the early 1900’s on the Cocos-Keeling Islands and Christmas Island, where they have now caused a massive decline in the population of iconic red crabs. More recently, they have been recorded in Queensland, the Northern Territory (NT), and New South Wales (NSW).

Adopt-A-Trap To Help Detect European Wasps, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity factsheets

Early detection of European wasp is important to prevent the spread and establishment of nests, and the Adopt-a-trap program is a key part of annual European wasp surveillance.

The department intensively traps the highest risk areas of the state, but the Adopt-a-trap program can extend surveillance beyond these areas and into the regions to enhance community surveillance.

The trapping method used in the Adopt-a-trap program is very successful in catching European wasps. They address the wasps’ sense of smell and foraging habits to find food. Trapped wasps are useful in alerting us to the presence of a nearby nest — …

Argentine Ants, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity factsheets

Argentine ants (Linepithema humile) are small, brown, and notorious worldwide for being one of the most difficult and costly ants to control, as it can involve ongoing and repetitive use of residual, contact insecticides. The ants are established in Western Australia (WA).

Electric Ant (Little Fire Ant), Department Of Primary Industries And Regional Development, Western Australia

Biosecurity factsheets

The electric ant (Wasmannia auropunctata), also known as little fire ant, is a tiny, gold-brown coloured ant, and one of the world's most invasive pests. Electric ants prey on and compete with other insects and can displace large numbers of native animals, ants, and other insects.

They affect human health and lifestyle, can inflict painful stings on humans and wild and domestic animals, can blind pets, damage the environment, and have the potential to severely affect agricultural industries.

The electric ant is native to Central and South America and was first detected in Australia in 2006 in Queensland, where there …