Entomology Commons^™

Larval Performance And Kill Rate Of Convergent Ladybird Beetles, Hippodamia Convergens, On Black Bean Aphids, Aphis Fabae, And Pea Aphids, Acyrthosiphon Pisum, Travis M. Hinkelman, Brigitte Tenhumberg

School of Biological Sciences: Faculty Publications

Generalist predator guilds play a prominent role in structuring insect communities and can contribute to limiting population sizes of insect pest species. A consequence of dietary breadth, particularly in predatory insects, is the inclusion of low-quality, or even toxic, prey items in the predator’s diet. Consumption of low-quality prey items reduces growth, development, and survival of predator larvae, thereby reducing the population sizes of generalist predators. The objective of this paper was to examine the effect of a suspected low-quality aphid species, Aphis fabae (Scopoli) (Hemiptera: Aphididae), on the larval performance of an abundant North American predator, Hippodamia convergens (Guérin-Méneville) …

Kinematic Analysis Of Axial Rotations And The Effects Of Stress Selection On Takeoff Flight Performance, Michael L. Brewer

UNLV Theses, Dissertations, Professional Papers, and Capstones

The importance of flying insects cannot be understated; without them as pollinators, the great diversity of flowing plants could not have occurred. Flight requires a suite of highly derived morphological and physiological characteristics that may limit the evolutionary responses of other life history traits.

To investigate the complexities of maneuvering flight, I used high-speed videography to analyze wing and body motions in the HoverflySyritta pipiens(Order: Diptera) during horizontal turns called saccades. I characterized the saccades by calculating the instantaneous rotational velocities throughout the saccade, maximum rotational velocity, and the mean rotational velocity. I then compared the shape of the rotational …

Studies On The Structure And Pathogens Of The Small Hive Beetle (Coleoptera: Nitidulidae), Natasha A. Wright

Graduate Theses and Dissertations

The small hive beetle (Aethina tumida, SHB) is an invasive pest of honey bee (Apis mellifera) colonies in the United States. The adult and larval beetles can ruin honey through fecal contamination and by vectoring a mutualistic yeast (Kodamaea ohmeri) that causes honey fermentation. These beetles also impact honey bee colonies by feeding on bee eggs, bee brood, and pollen. Severe beetle infestations can cause colonies to decline or abscond.

The SHB has been present in the United States since at least 1998. Since then, there have been several published papers on how to successfully rear these beetles. Laboratory rearing …

Mitochondrial Genomes Of Two Barklice, Psococerastis Albimaculata And Longivalvus Hyalospilus (Psocoptera: Psocomorpha): Contrasting Rates In Mitochondrial Gene Rearrangement Between Major Lineages Of Psocodea, Hu Li, Renfu Shao, Fan Song, Xuguo Zhou, Qianqian Yang, Zhihong Li, Wanzhi Cai

Entomology Faculty Publications

The superorder Psocodea has ∼10,000 described species in two orders: Psocoptera (barklice and booklice) and Phthiraptera (parasitic lice). One booklouse, Liposcelis bostrychophila and six species of parasitic lice have been sequenced for complete mitochondrial (mt) genomes; these seven species have the most rearranged mt genomes seen in insects. The mt genome of a barklouse, lepidopsocid sp., has also been sequenced and is much less rearranged than those of the booklouse and the parasitic lice. To further understand mt gene rearrangements in the Psocodea, we sequenced the mt genomes of two barklice, Psococerastis albimaculata and Longivalvus hyalospilus, the first representatives from …

Differential Undertaking Response Of A Lower Termite To Congeneric And Conspecific Corpses, Qian Sun, Kenneth F. Haynes, Xuguo Zhou

Entomology Faculty Publications

Undertaking behaviour is an essential activity in social insects. Corpses are often recognized by a postmortem change in a chemical signature. Reticulitermes flavipes responded to corpses within minutes of death. This undertaking behaviour did not change with longer postmortem time (24 h); however, R. flavipes exhibited distinctively different behaviours toward dead termites from various origins. Corpses of the congeneric species, Reticulitermes virginicus, were buried onsite by workers with a large group of soldiers guarding the burial site due to the risk of interspecific competition; while dead conspecifics, regardless of colony origin, were pulled back into the holding chamber for nutrient …

Characterization Of Resistance To The Cry1f Toxin From Bacillus Thuringiensis In Resistant Fall Armyworm, Spodoptera Frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) From Puerto Rico, Ana Maria Velez

Department of Entomology: Dissertations, Theses, and Student Research

Transgenic corn expressing Cry1F protein from Bacillus thuringiensis Berliner has been registered for Spodoptera frugiperda control since 2003. Unexpected damage to Cry1F corn was reported in 2006 in Puerto Rico and Cry1F resistance in S. frugiperda was documented. The inheritance of Cry1F resistance was characterized in a S. frugiperda resistant strain from Puerto Rico which displayed >387-fold resistance to Cry1F. Concentration-response bioassays of reciprocal crosses of resistant and susceptible parental populations indicated that resistance is recessive and autosomal. Bioassays of the backcross of the F₁ generation crossed with the resistant parental strain suggest that a single locus is responsible …

The Endosymbiont Arsenophonus Is Widespread In Soybean Aphid, Aphis Glycines, But Does Not Provide Protection From Parasitoids Or A Fungal Pathogen, Jason A. Wulff, Karrie A. Buckman, Kongming Wu, George E. Heimpel, Jennifer A. White

Entomology Faculty Publications

Aphids commonly harbor bacterial facultative symbionts that have a variety of effects upon their aphid hosts, including defense against hymenopteran parasitoids and fungal pathogens. The soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), is infected with the symbiont Arsenophonus sp., which has an unknown role in its aphid host. Our research goals were to document the infection frequency and diversity of the symbiont in field-collected soybean aphids, and to determine whether Arsenophonus is defending soybean aphid against natural enemies. We performed diagnostic PCR and sequenced four Arsenophonus genes in soybean aphids from their native and introduced range to estimate infection frequency …

Parasitological Prevalence Of Bovine Trypanosomosis In Kubo Division Of Kwale County Of Coastal: Baseline Survey, N. Mbahin, H. Affognon, J. Andoke, M. Tiberius, D. Mbuvi, J. Otieno, P. Muasa, R. K. Saini

All Peer-Reviewed Publications

Prior to field trials of large scale tsetse repellent technology validation in July 2011, a cross-sectional survey of bovine trypanosomosis and its vectors was carried out to know the disease level in Kwale County of Coastal Kenya. A total of 584 adult cattle were examined at ten different Locations. The trypanosome prevalence in cattle with regard to age, sex, location and apparent tsetse density was investigated. The overall trypanosome prevalence in cattle in Kubo Division of Kwale County was 33.9%. Two types of infections were recorded in various parts of the Division in the following proportions: T. congolense 61.1%, T. …

Laboratory Bioassays To Estimate The Lethal And Sublethal Effects Of Various Insecticides And Fungicides On Deraeocoris Brevis (Hemiptera: Miridae), K. G. Amarasekare, P. W. Shearer

Agricultural and Environmental Sciences Faculty Research

This laboratory bioassay focused on lethal and sublethal effects of five insecticides (chlorantraniliprole, cyantraniliprole, spinetoram, novaluron, and lambda-cyhalothrin) and two fungicide treatments (sulfur and a mixture of copper hydroxide and mancozeb) on the predatory mired bug, Deraeocoris brevis (Uhler) (Hemiptera: Miridae) using second instars and adult males and females. Formulated pesticides were tested using concentrations that were equivalent to the high label rate (1x) (high rate) and 1/10th of that amount (0.1x) (low rate) dissolved in 378.5 liters of water. Lambda-cyhalothrin was highly toxic to D. brevis nymphs and adults at both rates, whereas both rates of novaluron were highly …

Hexapod Herald - Vol. 25, No. 2, April 2013

Hexapod Herald and Other Entomology Department Newsletters

Contents: Welcome - Congratulations - Grants - Meet an Off Campus Student - Meeting/Workshop News - Publications - Faculty News - Travel - Blasts from the Past - Calendar of Events

Forest Fragments As Barriers To Fruit Fly Dispersal: Anastrepha (Diptera: Tephritidae) Populations In Orchards And Adjacent Forest Fragments In Puerto Rico, David A. Jenkins, Paul E. Kendra, Skip Van Bloem, Stefanie Whitmire, Russ Mizell Iii, Ricardo Goenaga

Publications

McPhail-type traps baited with ammonium acetate and putrescine were used to monitor populations of Anastrepha obliqua (Macquart) and Anastrepha suspensa(Loew) in two orchards with hosts of these flies (mango, Mangifera indica L., and carambola, Averrhoa carambola L.), as well as in forest fragments bordering these orchards. Contour maps were constructed to measure population distributions in and around orchards. Our results indicate that Anastrephapopulations are focused around host fruit in both space and time, that traps do not draw fruit flies away from hosts, even when placed within 15 m of the host, and that lures continue to function …

Nebline, April 2013

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Landscapes in Drought

Farm Views

Urban Agriculture

Food & Fitness

Home & Family Living

Horticulture

Environmental Focus

4-H & Youth

Community Focus

Extension Calendar

Nutrition Education Program

Family and Community Education (FCE) Clubs

and other extension news and events

Weed Awareness Special Pullout Section

Carrion Beetle (Coleoptera: Silphidae) Carcass Selection: Effects Of Carcass Mass And Site Fidelity, Jonathan M. Schmude

Biological Sciences Theses & Dissertations

This study investigated reproductive behaviours of the carrion beetles (Coleoptera: Silphidae) of southeastern Virginia. Carrion beetles have been known to have ranges of carcass masses used for reproductive behaviors. In this study, pitfall traps were baited with 25g, 50g, or 75g of a cat food/mackerel mixture and left to decay and attract beetles for one week to investigate if any Silphidae species prefer a single mass over another. This study investigated the carcass bait mass that is used on the subfamily and species level, and how the beetle mass plays a role in preference. The study also investigated the effect …

Corpse Management In Social Insects, Qian Sun, Xuguo Zhou

Entomology Faculty Publications

Undertaking behavior is an essential adaptation to social life that is critical for colony hygiene in enclosed nests. Social insects dispose of dead individuals in various fashions to prevent further contact between corpses and living members in a colony. Focusing on three groups of eusocial insects (bees, ants, and termites) in two phylogenetically distant orders (Hymenoptera and Isoptera), we review mechanisms of death recognition, convergent and divergent behavioral responses toward dead individuals, and undertaking task allocation from the perspective of division of labor. Distinctly different solutions (e.g., corpse removal, burial and cannibalism) have evolved, independently, in the holometabolous hymenopterans and …

Sex-Specific Induction Of Cyp6 Cytochrome P450 Genes In Cadmium And Lead Tolerant Anopheles Gambiae, Fauzia K. Musasia, Alfred O. Isaac, Daniel K. Masiga, Irene A. Omedo, Ramadhan Mwakubambanya, Richard Ochieng, Paul O. Mireji

All Peer-Reviewed Publications

Background: Anopheles gambiae, one of the main Afro-tropical mosquito vector of malaria, has adapted to heavy metals in its natural habitat, and developed resistance to most conventional insecticides. Investigations were conducted to establish an association between tolerance to cadmium or lead-heavy metals, and expression of specific genes for cytochrome p450 enzymes associated with pyrethroid resistance in the mosquito. Methods. Juvenile aquatic stages of the mosquito were selected for tolerance to cadmiun or lead through chronic exposure of the stages to maximum acceptable toxicant concentrations (MATCs) of the metals. Using real-time quantitative polymerase chain reaction (qPCR), three replicates each of male …

Bed Bugs Evolved Unique Adaptive Strategy To Resist Pyrethroid Insecticides, Fang Zhu, Hemant Gujar, Jennifer R. Gordon, Kenneth F. Haynes, Michael F. Potter, Subba R. Palli

Entomology Faculty Publications

Recent advances in genomic and post-genomic technologies have facilitated a genome-wide analysis of the insecticide resistance-associated genes in insects. Through bed bug, Cimex lectularius transcriptome analysis, we identified 14 molecular markers associated with pyrethroid resistance. Our studies revealed that most of the resistance-associated genes functioning in diverse mechanisms are expressed in the epidermal layer of the integument, which could prevent or slow down the toxin from reaching the target sites on nerve cells, where an additional layer of resistance (kdr) is possible. This strategy evolved in bed bugs is based on their unique morphological, physiological and behavioral characteristics …

Integrated Analysis Of Cytochrome P450 Gene Superfamily In The Red Flour Beetle, Tribolium Castaneum, Fang Zhu, Timothy W. Moural, Kapil Shah, Subba Reddy Palli

Entomology Faculty Publications

BACKGROUND: The functional and evolutionary diversification of insect cytochrome P450s (CYPs) shaped the success of insects. CYPs constitute one of the largest and oldest gene superfamilies that are found in virtually all aerobic organisms. Because of the availability of whole genome sequence and well functioning RNA interference (RNAi), the red flour beetle, Tribolium castaneum serves as an ideal insect model for conducting functional genomics studies. Although several T. castaneum CYPs had been functionally investigated in our previous studies, the roles of the majority of CYPs remain largely unknown. Here, we comprehensively analyzed the phylogenetic relationship of all T. castaneum CYPs …

A Distribution Of Dragonflies And Damselflies (Odonata) Of Louisiana, George G. Sims

Midsouth Entomologist

No abstract provided.

2012 Soybean Insect Losses In The Southern Us, F. R. Musser, A. L. Catchot Jr., J. A. Davis, D. A. Herbert Jr., G. M. Lorenz, T. Reed, D. D. Reisig, S. D. Stewart

Midsouth Entomologist

Survey-based soybean insect losses were collected following the 2012 growing season to provide a record of insect pressure and soybean management practices for the year. This survey has been done annually in participating states for the last 2-9 years. The 2012 survey represents more than 10 million acres across the southern United States. Overall, the 2012 survey showed corn earworm to be the most costly insect pest in the region for the second consecutive year, costing growers more than $20/acre in direct costs and yield loss. Soybean looper and stink bug were the two other major pests during 2012. More …

Current Topics In Medical, Urban, And Veterinary Entomology: 4th Annual Roundtable Discussion, K. T. Edwards

Midsouth Entomologist

No abstract provided.

Using Seed Mixes In Conjunction With Structured Refuges, M. A. Caprio, J. C. Martinez

Midsouth Entomologist

The U.S. Environmental Protection Agency recently approved, for areas of the Southern U.S., a 5% seed-mixture (Refuge-In-A-Bag or RIB) combined with a 20% external structured refuge for pyramided Bt corn. In this analysis we compared the durability of a pyramided PIP (plant incorporated protectant) deployed with a 20% refuge versus a 5% RIB and an additional 20% external refuge when the larval movement proportion was varied. The results showed that when the PIP expressed a very high dose (overall mortality of susceptibles = 99.99%) and larval movement was 50% and 90%, the 20% external refuge was 38% and 52% more …

Nebline, March 2013

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Dryland Farming in a Drought

Farm Views

Urban Agriculture

Food & Fitness

Home & Family Living

Horticulture

Environmental Focus

4-H & Youth

Community Focus

Extension Calendar

Nutrition Education Program

Family and Community Education (FCE) Clubs

and other extension news and events

Population Impacts Of Wolbachia On Aedes Albopictus, James W. Mains, Corey L. Brelsfoard, Philip R. Crain, Yunxin Huang, Stephen L. Dobson

Entomology Faculty Publications

Prior studies have demonstrated that Wolbachia, a commonly occurring bacterium capable of manipulating host reproduction, can affect life history traits in insect hosts, which in turn can have population-level effects. Effects on hosts at the individual level are predicted to impact population dynamics, but the latter has not been examined empirically. Here, we describe a biological model system based on Aedes albopictus (Asian tiger mosquito) that allows for measurement of population dynamics, which has not been accomplished in prior field trials or laboratory designs. The results demonstrate the studied populations to be robust and allow for persistent, closed populations with …

Program And Abstracts Of The 59th Annual Conference Of The Mississippi Entomological Association, October 22-23, 2012, Mississippi Entomological Association

Midsouth Entomologist

No abstract provided.

Parasitoid Infestation Changes Female Mating Preferences, Oliver M. Beckers, William E. Wagner

School of Biological Sciences: Faculty Publications

Females often adjust their mating preference to environmental and social conditions. This plasticity of preference can be adaptive for females and can have important consequences for the evolution of male traits. While predation and parasitism are widespread, their effects on female preferences have rarely been investigated. Females of the cricket Gryllus lineaticeps are parasitized by the parasitoid fly Ormia ochracea. Infestation with fly larvae substantially reduces female life span and thus reproductive opportunities of the cricket. Both female G. lineaticeps and flies orient to male song and both prefer male songs with faster chirp rates to songs with slower chirp …

The Functioning Of Species-Specific Olfactory Pheromones In The Biology Of A Mosquito-Eating Jumping Spider From East Africa, Fiona R. Cross, Robert R. Jackson

All Peer-Reviewed Publications

Having unique, complex eyes and vision based on exceptional spatial acuity, salticid spiders are known for their vision-based predatory and mating strategies. Yet Evarcha culicivora, the salticid we consider here, is known for predatory and mating strategies based strongly on the interplay of vision and olfaction. This unusual East African species feeds indirectly on vertebrate blood by choosing blood-carrying female mosquitoes as preferred prey, which it can identify by sight and by smell. Moreover, E. culicivora's mating strategy is unusual because, unlike the prevailing general pattern among spiders, where males are more active in courtship and females are more active …

Improving Sterile Insect Technique (Sit) For Tsetse Flies Through Research On Their Symbionts And Pathogens, Adly M.M. Abd-Alla, Max Bergoin, Andrew G. Parker, Nguya K. Maniania, Just M. Vlak, Kostas Bourtzis, Drion G. Boucias, Serap Aksoy

All Peer-Reviewed Publications

Tsetse flies (Diptera: Glossinidae) are the cyclical vectors of the trypanosomes, which cause human African trypanosomosis (HAT) or sleeping sickness in humans and African animal trypanosomosis (AAT) or nagana in animals. Due to the lack of effective vaccines and inexpensive drugs for HAT, and the development of resistance of the trypanosomes against the available trypanocidal drugs, vector control remains the most efficient strategy for sustainable management of these diseases. Among the control methods used for tsetse flies, Sterile Insect Technique (SIT), in the frame of area-wide integrated pest management (AW-IPM), represents an effective tactic to suppress and/or eradicate tsetse flies. …

Repellent, Irritant And Toxic Effects Of 20 Plant Extracts On Adults Of The Malaria Vector Anopheles Gambiae Mosquito, Emilie Deletre, Thibaud Martin, Pascal Campagne, Denis Bourguet, Andy Cadin, Chantal Menut, Romain Bonafos, Fabrice Chandre

All Peer-Reviewed Publications

Pyrethroid insecticides induce an excito-repellent effect that reduces contact between humans and mosquitoes. Insecticide use is expected to lower the risk of pathogen transmission, particularly when impregnated on long-lasting treated bednets. When applied at low doses, pyrethroids have a toxic effect, however the development of pyrethroid resistance in several mosquito species may jeopardize these beneficial effects. The need to find additional compounds, either to kill disease-carrying mosquitoes or to prevent mosquito contact with humans, therefore arises. In laboratory conditions, the effects (i.e., repellent, irritant and toxic) of 20 plant extracts, mainly essential oils, were assessed on adults of Anopheles gambiae, …

Wolbachia Association With The Tsetse Fly, Glossina Fuscipes Fuscipes, Reveals High Levels Of Genetic Diversity And Complex Evolutionary Dynamics, Rebecca E. Symula, Uzma Alam, Corey L. Brelsfoard, Yineng Wu, Richard Echodu, Loyce M. Okedi, Serap Aksoy, Adalgisa Caccone

Entomology Faculty Publications

BACKGROUND: Wolbachia pipientis, a diverse group of α-proteobacteria, can alter arthropod host reproduction and confer a reproductive advantage to Wolbachia-infected females (cytoplasmic incompatibility (CI)). This advantage can alter host population genetics because Wolbachia-infected females produce more offspring with their own mitochondrial DNA (mtDNA) haplotypes than uninfected females. Thus, these host haplotypes become common or fixed (selective sweep). Although simulations suggest that for a CI-mediated sweep to occur, there must be a transient phase with repeated initial infections of multiple individual hosts by different Wolbachia strains, this has not been observed empirically. Wolbachia has been found in the tsetse fly, Glossina …

Ethanol Injection Of Ornamental Trees Facilitates Testing Insecticide Efficacy Against Ambrosia Beetles (Coleoptera: Curculionidae: Scolytinae), Michael E. Reding, Jason B. Oliver, Peter B. Schultz, Christopher M. Ranger, Nadeer N. Youssef

Agricultural and Environmental Sciences Faculty Research

Exotic ambrosia beetles are damaging pests in ornamental tree nurseries in North America. The species Xylosandrus crassiusculus (Motshulsky) and Xylosandrus germanus (Blandford) are especially problematic. Management of these pests relies on preventive treatments of insecticides. However, field tests of recommended materials on nursery trees have been limited because of unreliable attacks by ambrosia beetles on experimental trees. Ethanol-injection of trees was used to induce colonization by ambrosia beetles to evaluate insecticides and botanical formulations for preventing attacks by ambrosia beetles. Experiments were conducted in Ohio, Tennessee, and Virginia. Experimental trees injected with ethanol had more attacks by ambrosia beetles than …