Entomology Commons^™

Dominant Inheritance Of Field-Evolved Resistance To Bt Corn In Busseola Fusca, Pascal Campagne, Marlene Kruger, Rémy Pasquet, Bruno Le Ru, Johnnie Van Den Berg

All Peer-Reviewed Publications

Transgenic crops expressing Bacillus thuringiensis (Bt) toxins have been adopted worldwide, notably in developing countries. In spite of their success in controlling target pests while allowing a substantial reduction of insecticide use, the sustainable control of these pest populations is threatened by the evolution of resistance. The implementation of the "high dose/refuge" strategy for managing insect resistance in transgenic crops aims at delaying the evolution of resistance to Bt crops in pest populations by promoting survival of susceptible insects. However, a crucial condition for the "high dose/refuge" strategy to be efficient is that the inheritance of resistance should be functionally …

Nebline, July 2013

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Integrated Pest Management in Nebraska Schools: Solving Pest Problems Effectively with Fewer Pesticides

Farm Views

Urban Agriculture

Food & Fitness

Home & Family Living

Horticulture

Environmental Focus

4-H & Youth

Community Focus

Extension Calendar

Nutrition Education Program

Family and Community Education (FCE) Clubs

and other extension news and events

The Mayfly Newsletter, Peter M. Grant

The Mayfly Newsletter

The Mayfly Newsletter is the official newsletter of the Permanent Committee of the International Conferences on Ephemeroptera.

Waveform Library For Chinch Bugs (Hemiptera: Heteroptera: Blissidae): Characterization Of Electrical Penetration Graph Waveforms At Multiple Input Impedances, Elaine A. Backus, Murugesan Rangasamy, Mitchell Stamm, Heather J. Mcauslane, Ron Cherry

Department of Entomology: Faculty Publications

Electrical penetration graph (EPG) monitoring has been used extensively to elucidate mechanisms of resistance in plants to insect herbivores with piercing-sucking mouthparts. Characterization of waveforms produced by insects during stylet probing is essential to the application of this technology. In the studies described herein, a four-channel Backus and Bennett AC-DC monitor was used to characterize EPG waveforms produced by adults of two economically important chinch bug species: southern chinch bug, Blissus insularis Barber, feeding on St. Augustinegrass, and western chinch bug, Blissus occiduus Barber, feeding on buffalograss. This is only the third time a heteropterans species has been recorded by …

Systematics And Distributions Of The Genera Cyrtusa Erichson, Ecarinosphaerula Hatch, Isoplastus Horn, Liocyrtusa Daffner, Lionothus Brown, And Zeadolopus Broun Of The United States And Canada (Coleoptera: Leiodidae: Leiodinae: Leiodini), Stewart B. Peck, Joyce Cook

Insecta Mundi

The following genera of Leiodini (Coleoptera: Leiodidae: Leiodinae) of the continental United States and Canada are reviewed: Cyrtusa Erichson, with two species; Isoplastus Horn, with two species (one new); Liocyrtusa Daffner, with three species; Lionothus Brown, with five species (three new), and Zeadolopus Broun, with four species (all genera are in the “Cyrtusa genus group”) and Ecarinosphaerula Hatch, with one named species (in the “Leiodes genus group”). The new species are Isoplastus floridanus Peck and Cook of Florida; Lionothus bidentatus Peck and Cook of Texas and Oklahoma, Lionothus exiguus Peck and Cook of Florida and Texas, and Lionothus …

Candidate Chemosensory Genes In The Stemborer Sesamia Nonagrioides, Nicolas Glaser, Aurore Gallot, Fabrice Legeai, Nicolas Montagné, Erwan Poivet, Myriam Harry, Paul André Calatayud, Emmanuelle Jacquin-Joly

All Peer-Reviewed Publications

The stemborer Sesamia nonagrioides is an important pest of maize in the Mediterranean Basin. Like other moths, this noctuid uses its chemosensory system to efficiently interact with its environment. However, very little is known on the molecular mechanisms that underlie chemosensation in this species. Here, we used next-generation sequencing (454 and Illumina) on different tissues from adult and larvae, including chemosensory organs and female ovipositors, to describe the chemosensory transcriptome of S. nonagrioides and identify key molecular components of the pheromone production and detection systems. We identified a total of 68 candidate chemosensory genes in this species, including 31 candidate …

Effects Of Flight On Gene Expression And Aging In The Honey Bee Brain And Flight Muscle, Joseph W. Margotta, Georgina E. Mancinelli, Azucena A. Benito, Andrew Ammons, Stephen P. Roberts, Michelle M. Elekonich

Biological Sciences Faculty Research & Creative Works

Honeybees Move through a Series of In-Hive Tasks (E.g., "Nursing") to Outside Tasks (E.g., "Foraging") that Are Coincident with Physiological Changes and Higher Levels of Metabolic Activity. Social Context Can Cause Worker Bees to Speed Up or Slow Down This Process, and Foragers May Revert Back to their Earlier In-Hive Tasks Accompanied by Reversion to Earlier Physiological States. to Investigate the Effects of Flight, Behavioral State and Age on Gene Expression, We Used Whole-Genome Microarrays and Real-Time PCR. Brain Tissue and Flight Muscle Exhibited Different Patterns of Expression during Behavioral Transitions, with Expression Patterns in the Brain Reflecting Both Age …

Assessing Insecticide Hazard To Bumble Bees Foraging On Flowering Weeds In Treated Lawns, Jonathan L. Larson, Carl T. Redmond, Daniel A. Potter

Entomology Faculty Publications

Maintaining bee-friendly habitats in cities and suburbs can help conserve the vital pollination services of declining bee populations. Despite label precautions not to apply them to blooming plants, neonicotinoids and other residual systemic insecticides may be applied for preventive control of lawn insect pests when spring-flowering weeds are present. Dietary exposure to neonicotinoids adversely affects bees, but the extent of hazard from field usage is controversial. We exposed colonies of the bumble bee Bombus impatiens to turf with blooming white clover that had been treated with clothianidin, a neonicotinoid, or with chlorantraniliprole, the first anthranilic diamide labeled for use on …

Horizontal Transmission Of Metarhizium Anisopliae In Fruit Flies And Effect Of Fungal Infection On Egg Laying And Fertility, Susan Dimbi, Nguya K. Maniania, Sunday Ekesi

All Peer-Reviewed Publications

Fly-to-fly transmission of conidia of the entomopathogenic fungus Metarhizium anisopliae and the effect of fungal infection on the reproductive potential of females surviving infection were investigated in three fruit fly species, Ceratitis cosyra, C. fasciventris, and C. capitata. The number of conidia picked up by a single fruit fly was determined in C. cosyra. The initial uptake (Day 0) of conidia by a single fly was approx. 1.1 × 106 conidia after exposure to the treated substrate. However, the number of conidia dropped from 7.2 × 105 to 4.1 × 105 conidia after 2 and 8 h post-exposure, respectively. The …

Ectoparasites Of The Virginia Opossum (Didelphis Virginiana), Raccoon (Procyon Lotor), And Striped Skunk (Mephitis Mephitis) From Keith County, Nebraska, Lance A. Durden, Dennis J. Richardson

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Six Virginia opossums (Didelphis virginiana), nine raccoons (Procyon lotor) and one striped skunk (Mephitis mephitis) collected from Keith County Nebraska were examined for ectoparasites. All three host species were parasitized by adults of the American dog tick, Dermacentor variabilis. Opossums were also parasitized by the flea Pulex simulans and the tiny fur mite Didelphilichus serri­fer; the latter species represents a new state record for Nebraska. Raccoons were also parasitized by P. simulans and by the lago­morph-associated flea Euhoplopsyllus glacialis affinis, whereas the skunk was also parasitized by the chewing louse Neotrichodectes …

Juvenile Hormone And Insulin Regulate Trehalose Homeostasis In The Red Flour Beetle, Tribolium Castaneum, Jingjing Xu, Zhentao Sheng, Subba R. Palli

Entomology Faculty Publications

Insulin/IGF-1 signaling (IIS) has been well studied for its role in the control of life span extension and resistance to a variety of stresses. The Drosophila melanogaster insulin-like receptor (InR) mutant showed extended life span due to reduced juvenile hormone (JH) levels. However, little is known about the mechanism of cross talk between IIS and JH in regulation of life span extension and resistance to starvation. In the current study, we investigated the role of IIS and JH signaling in regulation of resistance to starvation. Reduction in JH biosynthesis, JH action, or insulin-like peptide 2 (ILP2) syntheses by RNA interference …

Preharvest Quarantine Treatments Of Chlorantraniliprole, Clothianidin, And Imidacloprid-Based Insecticides For Control Of Japanese Beetle (Coleoptera: Scarabaeidae) And Other Scarab Larvae In The Root Zone Of Field-Grown Nursery Trees, Jason B. Oliver, Christopher M. Ranger, Michael E. Reding, James J. Moyseenko, Nadeer N. Youssef, Alicia M. Bray

Agricultural and Environmental Sciences Faculty Research

Japanese beetle, Popillia japonica Newman (Coleoptera: Scarabaeidae), is an important quarantine pest of nurseries. Nursery plant movement from P. japonica-infested regions is regulated by the U.S. Domestic Japanese Beetle Harmonization Plan (DJHP), which classifies states by risk categories. Treatments for category 2 states include preharvest soil surface treatment of nursery plants grown in field soil using Discus SC, Marathon (1G or 60 WP), or Flagship (0.22G or 25 WG). In this study, Discus, Marathon 60 WP, or Flagship 0.22G DJHP standards were compared with labeled rates of non–DJHP-approved insecticides, including neonicotinoids clothianidin (Arena 50WDG), generic imidacloprid (Quali-Pro Imidacloprid 2 F …

Comparing Effects Of Insecticides On Two Green Lacewings Species, Chrysoperla Johnsoni And Chrysoperla Carnea (Neuroptera: Chrysopidae), K. G. Amarasekare, P. W. Shearer

Agricultural and Environmental Sciences Faculty Research

This study compared lethal and sublethal effects of five insecticides, chlorantraniliprole, cyantraniliprole, spinetoram, novaluron, and lambda-cyhalothrin, on adult and second instars of two green lacewing species, Chrysoperla carnea (Stephens) and Chrysoperla johnsoni Henry, Wells and Pupedis (Neuroptera: Chrysopidae) in the laboratory. Formulated pesticides were tested using concentrations equivalent to the high label rate dissolved in 378.5 liters of water. Novaluron and lambda-cyhalothrin were toxic to larvae and no treated larvae survived to the adult stage. Larva to adult survival was reduced in chlorantraniliprole, cyantraniliprole, and spinetoram treatments. Larva to adult developmental time and sex ratio were not different among the …

Nebline, June 2013

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Smart Family Finances

Farm Views

Urban Agriculture

Food & Fitness

Home & Family Living

Horticulture

Environmental Focus

4-H & Youth

Community Focus

Extension Calendar

Nutrition Education Program

Family and Community Education (FCE) Clubs

and other extension news and events

Overwintering In New Zealand Stick Insects, Alice B. Dennis, Luke T. Dunning, Christopher J. Dennis, Brent J. Sinclair, Thomas R. Buckley

Biology Publications

Stick insects are found in a variety of habitats throughout New Zealand, including at least four species that occur at high altitudes. Here they face physiological challenges that differ from their typically warmer lowland habitats, but their strategies to deal with harsh winter conditions are not known. Autumn and winter field surveys, coupled with caging experiments, were conducted to determine which life stages are overwintering in montane and lowland habitats. Data loggers were placed for approximately one year at each site to measure the leaf litter and canopy microhabitat temperatures. From this, we have found that alpine and lowland stick …

Hexapod Herald - Vol. 25, No. 3, June 2013

Hexapod Herald and Other Entomology Department Newsletters

Contents: Welcome - Grants - North Central Branch News - Publications - Congratulations - Student News - Meeting an Off-Campus Student - This 'n That - Workshop News - Calendar of Events

Occurrence Of Entomopathogenic Fungi And Parasitic Nematodes On Spodoptera Frugiperda (Lepidoptera: Noctuidae) Larvae Collected In Central Chiapas, México, Ramiro E. Ruiz-Najera, Ramiro E. Ruiz-Estudillo, Juan M. Sanchez-Yanex, Jaime Molina-Ochoa, S. R. Skoda, Roberto Coutino-Ruiz, Rene Pinto-Ruiz, Francisco Guevara-Hernandez, John E. Foster

Department of Entomology: Faculty Publications

Fall armyworm larvae (FAW), Spodoptera frugiperda (J. E. Smith) were collected from whorl-stage corn, Zea mays (L.), between the V₂ and V₄ stages, in 22 localities of Central, Chiapas, México, called “La Frailesca” during late Jun 2009 to determine the occurrence of native entomopathogens and parasitic nematodes, and to determine the most parasitized instars. A total of 1,247 larvae were examined in search of native biological control agents. Overall total larval mortality was 16.36%. The percent larval mortality due to entomopathogens and parasitic nematodes was 12.99%. Hexamermis sp., probably albicans Siebold (Mermitha: Mermithidae) were isolated from 105 FAW …

Use Of Bee-Attractants In Increasing Crop Productivity In Niger (Guizotia Abyssinica. L), Venkataramegowda Sivaram, Koragandahalli Venkateshappa Jayaramappa, Anita Menon, Ruben Michael Ceballos

Biological Sciences Faculty Publications and Presentations

The use of bee attractants, Bee-Q and Fruit Boost™ in the pollination of niger was evaluated. Bee visitations to niger flowers were observed for two weeks and an estimation of seed yield was determined. Different concentrations of Bee-Q and Fruit boost™ were evaluated to understand honeybee visitation patterns on the target crop and pollination efficiency. Results indicate that applications of Bee-Q at 12.5 g/l and Fruit boost at 0.75 ml/l on niger plots significantly increased the number of bee foragers over control plots. In addition, plots sprayed with these bee attractants significantly enhanced the seed set, seed weight, and germination …

Composition Of Stem Borer Communities In Selected Vegetation Mosaics In Kenya, G. O. Ong'amo, B. P. Le Ru, P. A. Calatayud, J. F. Silvain

All Peer-Reviewed Publications

Busseola fusca (Fuller), Sesamia calamistis Hampson, Chilo partellus (Swinhoe) and Chilo orichalcociliellus (Strand) are important stem borer pests of maize and sorghum in East Africa. Persistence of these pests in crop fields is blamed on the influx of diaspore populations from the neighbouring natural habitats. In addition to pest species, natural habitats support numerous non-economic stem borer species, some not known to science. However, due to growing human populations and accompanying global change, some of the natural habitats are undergoing rapid changes, a process that may result in the evolution of "new" pest species. In this study, we investigated stem …

Transfer Of Inoculum Of Metarhizium Anisopliae Between Adult Glossina Morsitans Morsitans And Effects Of Fungal Infection On Blood Feeding And Mating Behaviors, Nguya K. Maniania, Matilda A. Okech, James O. Adino, Jacob O. Opere, Sunday Ekesi

All Peer-Reviewed Publications

The transfer of conidia of Metarhizium anisopliae between tsetse flies Glossina morsitans and the effects of fungal inoculation on mating and blood meal feeding behaviors were investigated in the laboratory. Male or female flies were inoculated with fungal conidia ("donors") and allowed to pair with fungus-free mate of opposite sex ("recipients") at 1-day-interval up to three mates. Fungus-treated male or female "donor" flies as well as their mates "recipients" died from fungal infection. However, mortality in male "recipient" flies declined with successive mating, from 82.5 to 32.5 %. Fungus-treated males readily located female flies and mating was successful in most …

Adaptive Selection On Bracovirus Genomes Drives The Specialization Of Cotesia Parasitoid Wasps, Séverine Jancek, Annie Bézier, Philippe Gayral, Corentin Paillusson, Laure Kaiser, Stéphane Dupas, Bruno Pierre Le Ru, Valérie Barbe, Georges Periquet, Jean Michel Drezen, Elisabeth A. Herniou

All Peer-Reviewed Publications

The geographic mosaic of coevolution predicts parasite virulence should be locally adapted to the host community. Cotesia parasitoid wasps adapt to local lepidopteran species possibly through their symbiotic bracovirus. The virus, essential for the parasitism success, is at the heart of the complex coevolutionary relationship linking the wasps and their hosts. The large segmented genome contained in the virus particles encodes virulence genes involved in host immune and developmental suppression. Coevolutionary arms race should result in the positive selection of particular beneficial alleles. To understand the global role of bracoviruses in the local adaptation or specialization of parasitoid wasps to …

Mosquito-Borne Arbovirus Surveillance At Selected Sites In Diverse Ecological Zones Of Kenya; 2007 - 2012, Caroline Ochieng, Joel Lutomiah, Albina Makio, Hellen Koka, Edith Chepkorir, Santos Yalwala, James Mutisya, Lillian Musila, Samoel Khamadi, Jason Richardson, Joshua Bast, David Schnabel, Eyako Wurapa, Rosemary Sang

All Peer-Reviewed Publications

Abstract. Background: Increased frequency of arbovirus outbreaks in East Africa necessitated the determination of distribution of risk by entomologic arbovirus surveillance. A systematic vector surveillance programme spanning 5 years and covering 11 sites representing seven of the eight provinces in Kenya and located in diverse ecological zones was carried out. Methods. Mosquitoes were sampled bi-annually during the wet seasons and screened for arboviruses. Mosquitoes were identified to species, pooled by species, collection date and site and screened for arboviruses by isolation in cell culture and/or RT-PCR screening and sequencing. Results: Over 450,000 mosquitoes in 15,890 pools were screened with 83 …

Pace Family Papers (Mss 458), Manuscripts & Folklife Archives

Manuscript Collection Finding Aids

Finding aid only for Manuscripts Collection 458. Pace family documents (chiefly related to land transactions) including deeds, land grants, mortgages; as well as Bible records for the Pace and related families (click on "Additional Files" below for scans), and probate records. A significant portion of the collection documents the garden club activities of Lillian (Tynes) Pace and her efforts to have the Viceroy butterfly designated as the Kentucky state butterfly.

Transcriptomic And Proteomic Responses Of Sweetpotato Whitefly, Bemisia Tabaci, To Thiamethoxam, Nina Yang, Wen Xie, Xin Yang, Shaoli Wang, Qingjun Wu, Rumei Li, Huipeng Pan, Baiming Liu, Xiaobin Shi, Yong Fang, Baoyun Xu, Xuguo Zhou, Youjun Zhang

Entomology Faculty Publications

BACKGROUND: The sweetpotato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), is one of the most widely distributed agricultural pests. Although it has developed resistance to many registered insecticides including the neonicotinoid insecticide thiamethoxam, the mechanisms that regulate the resistance are poorly understood. To understand the molecular basis of thiamethoxam resistance, "omics" analyses were carried out to examine differences between resistant and susceptible B. tabaci at both transcriptional and translational levels.

RESULTS: A total of 1,338 mRNAs and 52 proteins were differentially expressed between resistant and susceptible B. tabaci. Among them, 11 transcripts had concurrent transcription and translation profiles. KEGG analysis mapped 318 …

Metronidazole Effects On Hindgut Microflora Residing Within The Subterranean Termite Reticulitermes Flavipes, Alexandra Gjevre

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by Alexandra Gjevre on May 1, 2013.

Temporal Food Preference And Effectiveness Of Selected Bait Products Against Pachycondyla Chinensis (Emery) (Hymenoptera: Formicidae), Ying Mo

All Theses

Pachycondyla chinensis (Emery), commonly known as the Asian needle ant is a well-established invasive species in urban and woodland areas in South Carolina. Foraging ants are found around or under places such as sidewalks, flowerbeds, mulch, tree bases, stones, and logs where human outdoor activity takes place in urbanized area. It is not an aggressive ant, but it has a powerful sting that causes severe allergic reactions in some people. It also has a negative impact on native ant species in forest environments. Food preference was studied, followed by an evaluation of selected bait products against P. chinensis.
Protein, carbohydrate, …

An Automatic Framework For Embryonic Localization Using Edges In A Scale Space, Zachary Bessinger

Masters Theses & Specialist Projects

Localization of Drosophila embryos in images is a fundamental step in an automatic computational system for the exploration of gene-gene interaction on Drosophila. Contour extraction of embryonic images is challenging due to many variations in embryonic images. In the thesis work, we develop a localization framework based on the analysis of connected components of edge pixels in a scale space. We propose criteria to select optimal scales for embryonic localization. Furthermore, we propose a scale mapping strategy to compress the range of a scale space in order to improve the efficiency of the localization framework. The effectiveness of the proposed …

Nebline, May 2013

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Moving Toward a Healthier You

Farm Views

Urban Agriculture

Food & Fitness

Home & Family Living

Horticulture

Environmental Focus

4-H & Youth

Community Focus

Extension Calendar

Nutrition Education Program

Family and Community Education (FCE) Clubs

and other extension news and events

Influence Of Horn Flies And Breed Type On Milk Production, Calf Production Traits, Pasture Behavior, And Temperament Measurements In Beef Cattle, Angela Rene Mays

Graduate Theses and Dissertations

An experiment conducted in El Reno, Oklahoma during the fly season (May - September/October) evaluated the effects of horn flies on milk production, calf performance, and pasture behavior and temperament measurements of beef cow calf pairs. Cows (n = 53) sired by Bonsmara (BONS; n = 7), Brangus (BRAN; n = 13), Charolais (CHAR; n = 8), Gelbvieh (GELV; n = 5), Hereford (HERF; n = 12), and Romosinuano (ROMO; n = 8) from Brangus dams and their Angus sired calves (n = 51) were used in the study. Horn fly counts (HFC) and milk yield and quality estimates were …

The Life History Of Larinus Minutus, A Biological Control Agent Of Invasive Knapweeds, And Its Dispersal From Release Sites In Arkansas, Adam M. Alford

Graduate Theses and Dissertations

Larinus minutus Gyllenhal, a biological control agent of invasive knapweeds, has become established in several states and provinces since initial North American introduction in 1991. In order to reduce growing spotted knapweed populations in Northwest Arkansas, Larinus minutus (a biological control agent of spotted knapweed) was released annually from 2008-2011. Little is known about the larval development of this species, although the widespread use of this insect has provided research describing detailed host range and generalized life history. The speed and extent of the spread of this weevil from release sites following introduction have not been reported. This research described …