Entomology Commons^™

Evaluation Of Seed Treatments And At-Plant Soil Insecticides For The Control Of Wireworms (Coleoptera: Elateridae) In Field Corn, 2015, Kayla A. Mollet, Jonas Victor De Macedo, Grace E. Hirzel, C. Oliveira-Hofman, Julie A. Peterson

Department of Entomology: Faculty Publications

Wireworms are an important pest of corn, other field crop seedlings, and vegetables and can cause significant damage if not controlled. This field trial was established to evaluate the efficacy of neonicotinoid seed treatments and in-furrow soil insecticides to protect seedling field corn under a heavy wireworm pressure scenario. The trial was conducted on a commercial production field in Perkins County near Madrid, NE (40.781993° N, −101.463666° W). The field was selected for its likelihood to have heavy wireworm pressure due to the following: 1) past farmer observations of crop damage when planted to field corn in 2012; 2) sandy …

Spatial Variation In Western Corn Rootworm (Coleoptera: Chrysomelidae) Susceptibility To Cry3 Toxins In Nebraska, Jordan D. Reinders, Brianna D. Hitt, Walter W. Stroup, B. Wade French, Lance J. Meinke

Department of Entomology: Faculty Publications

Repeated use of field corn (Zea mays L.) hybrids expressing the Cry3Bb1 and mCry3A traits in Nebraska has selected for field-evolved resistance in some western corn rootworm (WCR; Diabrotica virgifera virgifera LeConte) populations. Therefore, this study was conducted to characterize spatial variation in local WCR susceptibility to Cry3Bb1 and mCry3A traits in Keith and Buffalo counties, Nebraska, and determine the relationship between past management practices and current WCR susceptibility. Adult WCR populations were collected from sampling grids during 2015 and 2016 and single-plant larval bioassays conducted with F₁ progeny documented significant variation in WCR susceptibility to Cry3Bb1 and …

Better Than Deet Repellent Compounds Derived From Coconut Oil, Junwei J. Zhu, Steven C. Cermak, James A. Kenar, Gary Brewer, Kenneth F. Haynes, David J. Boxler, Paul D. Baker, Desen Wang, Changlu Wang, Andrew Y. Li, Rui-De Xue, Yuan Shen, Fei Wang, Natasha M. Agramonte, Ulrich R. Bernier, Jaires G. De Oliveira Filho, Ligia M.F. Borges, Kristina Friesen, David B. Taylor

Department of Entomology: Faculty Publications

Hematophagous arthropods are capable of transmitting human and animal pathogens worldwide. Vector-borne diseases account for 17% of all infectious diseases resulting in 700,000 human deaths annually. Repellents are a primary tool for reducing the impact of biting arthropods on humans and animals. N,N-Diethyl-meta-toluamide (DEET), the most effective and long-lasting repellent currently available commercially, has long been considered the gold standard in insect repellents, but with reported human health issues, particularly for infants and pregnant women. In the present study, we report fatty acids derived from coconut oil which are novel, inexpensive and highly efficacious repellant compounds. These coconut fatty acids …

Seasonality Of Colaspis Crinicornis (Coleoptera: Chrysomelidae) And Its Injury Potential To Corn In Southeastern Nebraska, Kentaro Miwa, Lance Meinke

Department of Entomology: Faculty Publications

Colaspis crinicornis Schaeffer (Coleoptera: Chrysomelidae) primarily occurs in the Great Plains, United States. Although C. crinicornis has historically been considered a non-pest and is rarely found in agricultural systems, population densities of this species have been increasing in corn, Zea mays L., and soybean, Glycine max (L.) Merrill, over the last decade in southeastern Nebraska. As part of a comprehensive project to understand the life history and pest potential of C. crinicornis, a field study was conducted to: understand adult seasonality of C. crinicornis using emergence cages and whole-plant-count sampling in cornfields and sweep-net sampling in soybean fields; confirm …

Interactions Between Pesticides And Pathogen Susceptibility In Honey Bees, Scott T. O'Neal, Troy D. Anderson, Judy Wu-Smart

Department of Entomology: Faculty Publications

There exist a variety of factors that negatively impact the health and survival of managed honey bee colonies, including the spread of parasites and pathogens, loss of habitat, reduced availability or quality of food resources, climate change, poor queen quality, changing cultural and commercial beekeeping practices, as well as exposure to agricultural and apicultural pesticides both in the field and in the hive. These factors are often closely intertwined, and it is unlikely that a single stressor is driving colony losses. There is a growing consensus, however, that increasing prevalence of parasites and pathogens are among the most significant threats …

Arabidopsis Actin-Depolymerizing Factor3 Is Required For Controlling Aphid Feeding From The Phloem, Hossain A. Mondal, Joe Louis, Lani Archer, Monika Patel, Vamsi J. Nalam, Sujon Sarowar, Vishala Sivapalan, Douglas D. Root, Jyoti Shah

Department of Entomology: Faculty Publications

The actin cytoskeleton network has an important role in plant cell growth, division, and stress response. Actin-depolymerizing factors (ADFs) are a group of actin-binding proteins that contribute to reorganization of the actin network. Here, we show that the Arabidopsis (Arabidopsis thaliana) ADF3 is required in the phloem for controlling infestation by Myzus persicae Sülzer, commonly known as the green peach aphid (GPA), which is an important phloem sap-consuming pest of more than fifty plant families. In agreement with a role for the actin-depolymerizing function of ADF3 in defense against the GPA, we show that resistance in adf3 was …

Knockdown Of The Chromatin Remodeling Gene Brahma By Rna Interference Reduces Reproductive Fitness And Lifespan In Common Bed Bug (Hemiptera: Cimicidae), Sanjay Basnet, Shripat T. Kamble

Department of Entomology: Faculty Publications

The common bed bug, Cimex lectularius L. (Hemiptera: Cimicidae) is a nuisance household pest causing significant medical and economic impacts. RNA interference (RNAi) of genes that are involved in vital physiological processes can serve as potential RNAi targets for insect control. Brahma is an ATPase subunit of a chromatin-remodeling complex involved in transcription of several genes for cellular processes, most importantly the homeotic genes. In this study, we used a microinjection technique to deliver double stranded RNA into female bed bugs. Delivery of 0.05 and 0.5 μg/insect of brahma dsRNA directly into hemocele resulted substantial reduction in oviposition. Eggs laid …

Nebraska Growers’ And Crop Consultants’ Knowledge And Implementation Of Integrated Pest Management Of Western Bean Cutworm, Westen Ray Archibald, Jeff D. Bradshaw, Douglas A. Golick, Robert J. Wright, Julie A. Peterson

Department of Entomology: Faculty Publications

Western bean cutworm (WBC), Striacosta albicosta (Smith; Lepidoptera: Noctuidae), is a native noctuid pest of corn and dry beans in North America. While this pest has expanded its range greatly in recent years, historically it has consistently caused high yield losses in western Nebraska. A survey was distributed to growers, crop consultants and other agricultural professionals to obtain information about current management practices used for WBC. Questions covered multiple topics including: demographics, scouting practices, degree-day model use, confidence in management knowledge, Bt corn use, insecticide use, and considerations for biological control. There were 95 completed responses received by email. Respondents …

The First Record Of Ooencyrtus Nezarae (Hymenoptera: Encyrtidae) On Kudzu Bug (Hemiptera: Plataspidae) In North America, Blessing Ademokoya, Rammohan Balusu, Charles Ray, Jason Mottern, Henry Fadamiro

Department of Entomology: Faculty Publications

The kudzu bug, Megacopta cribraria (F.) (Heteroptera: Plataspidae), is an invasive insect pest introduced from Asia in 2009 that poses a threat to soybeans (Glycine max [L.] Merr. [Fabales: Fabaceae]) and other legume crops in the United States. Initially discovered in Georgia, M. cribraria rapidly expanded across the southeast until 2014 when a significant decline in its population was observed across many locations. This notable decline in M. cribraria populations is attributed to the emergence of new parasitoids and pathogens in its new invasive range. So far, only a single egg parasitoid, Paratelenomus saccharalis (Dodd) (Hymenoptera: Platygastridae), is known …

Morphological And Genetic Analysis Of Four Color Morphs Of Bean Leaf Beetle, Bamphitlhi Tiroesele, Steven R. Skoda, Thomas Hunt, Donald Lee, Muhammad I. Ullah, Jaime Molina-Ochoa, John E. Foster

Department of Entomology: Faculty Publications

Bean leaf beetle (BLB), Cerotoma trifurcata (Forster; Coleoptera: Chrysomelidae), exhibits considerable color variation but little is known about the underlying genetic structure and gene flow among color phenotypes. Genetic and morphological variation among four color phenotypes—green with spots (G+S), green without spots (G-S), red with spots (R+S) and red without spots (R-S)—were analyzed using amplified fragment length polymorphisms (AFLP) and morphometrics, respectively. AFLP generated 175 markers that showed ≥80% polymorphism. Analysis of molecular variance (AMOVA) indicated that genetic variation was greatest within phenotypes (82.6–84.0%); gene flow among the four phenotypes was relatively high (N_m = 3.82). The dendrogram and …

The Interface Between Wheat And The Wheat Curl Mite, Aceria Tosichella, The Primary Vector Of Globally Important Viral Diseases, Anna Skoracka, Brian G. Rector, Gary L. Hein

Department of Entomology: Faculty Publications

Wheat production and sustainability are steadily threatened by pests and pathogens in both wealthy and developing countries. This review is focused on the wheat curl mite (WCM), Aceria tosichella, and its relationship with wheat. WCM is a major pest of wheat and other cereals and a vector of at least four damaging plant viruses (Wheat streak mosaic virus, High plains wheat mosaic virus, Brome streak mosaic virus, and Triticum mosaic virus). The WCM–virus pathosystem causes considerable yield losses worldwide and its severity increases significantly when mixed-virus infections occur. Chemical control strategies are largely ineffective because WCM occupies …

Evaluation Of Greenbug And Yellow Sugarcane Aphid Feeding Behavior On Resistant And Susceptible Switchgrass Cultivars, Kyle G. Koch, Teresa Donze-Reiner, Lisa M. Baird, Joe Louis, Keenan L. Amundsen, Gautam Sarath, Jeff Bradshaw, Tiffany Heng-Moss

Department of Entomology: Faculty Publications

Switchgrass (Panicum virgatum L.) is an emerging biofuel crop that serves as host for aphids. To discern the effects of plant age and possible resistance mechanisms, the feeding behavior of greenbugs (Schizaphis graminum Rondani.) and the yellow sugarcane aphid (Sipha flava Forbes.) was monitored on three diverse switchgrasses by the electrical penetration graph (EPG) technique. Callose deposition and genes associated with callose metabolism were also analyzed to discern their association with plant resistance. There was a strong host effect on greenbugs feeding on lowland cultivar Kanlow at the V3 stage of development, as compared to the greenbug-susceptible …

The Influence Of Spatiotemporally Decoupled Land Use On Honey Bee Colony Health And Pollination Service Delivery, Matthew D. Smart, Clint R. V. Otto, Benjamin L. Carlson, Cali L. Roth

Department of Entomology: Faculty Publications

Societal dependence on insects for pollination of agricultural crops has risen amidst concerns over pollinator declines. Habitat loss and lack of forage have been implicated in the decline of both managed and native pollinators. Land use changes in the Northern Great Plains of the US, a region supporting over 1 million honey bee colonies annually, have shifted away from historical grassland ecosystems bees rely on for forage toward landscapes dominated by corn, soybeans, and other row crops. We investigated how land use impacts honey bee colony population size during the growing season and subsequent colony population size for almond pollination …

Clathrin-Dependent Endocytosis Is Associated With Rnai Response In The Western Corn Rootworm, Diabrotica Virgifera Virgifera Leconte, Daniele H. Pinheiro, Ana Maria Velez, Elane Fishilevich, Haichuan Wang, Newton P. Carneiro, Arnubio Valencia-Jimenez, Fernando H. Valicente, Kenneth E. Narva, Blair D. Siegfried

Department of Entomology: Faculty Publications

The cellular uptake of dsRNA after dietary exposure is critical for RNAi efficiency; however, the mechanism of its uptake in many insects remains to be understood. In this study, we evaluated the roles of the endocytic pathway genes Clathrin heavy chain (Chc), Clathrin adaptor protein AP50, ADP ribosylation factor-like 1 (Arf72A), Vacuolar H⁺ ATPase 16 kDa subunit (Vha16), and small GTPase Rab7 and putative sid-1-like genes (silA and silC) in RNAi response in western corn rootworm (WCR) using a two-stage dsRNA exposure bioassay. Silencing of Chc, Vha16, …

Abscisic And Jasmonic Acids Contribute To Soybean Tolerance To The Soybean Aphid (Aphis Glycines Matsumura), Kaitlin M. Chapman, Lia Marchi-Werle, Thomas E. Hunt, Tiffany M. Heng-Moss, Joe Louis

Department of Entomology: Faculty Publications

Plant resistance can provide effective, economical, and sustainable pest control. Tolerance to the soybean aphid has been identified and confirmed in the soybean KS4202. Although its resistance mechanisms are not fully understood, evidence suggests that enhanced detoxification of reactive oxygen species (ROS) is an active system under high aphid infestation. We further explored tolerance by evaluating the differences in constitutive and aphid-induced defenses in KS4202 through the expression of selected defense-related transcripts and the levels of the phytohormones abscisic acid (ABA), jasmonic acid (JA), JA-isoleucine (JA-Ile), cis-(+)-12-oxo-phytodienoic acid (OPDA), and salicylic acid (SA) over several time points. Higher constitutive levels …

Parental Rnai Suppression Of Chromatin Remodeling Genes To Control Coleopteran Pests, Blair D. Siegfried, Kenneth E. Narva, Kanika Arora, Sarah E. Worden, Chitvan Khajuria, Elane Fishilevich, Nicholas P. Storer, Meghan Frey, Ronda L. Hamm, Ana Maria Velez

Department of Entomology: Faculty Publications

This disclosure concerns nucleic acid molecules and meth ods of use thereof for control of hemipteran pests through RNA interference - mediated inhibition of target coding and transcribed non - coding sequences in hemipteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of hemipteran pests, and the plant cells and plants obtained thereby.

Breeding Patterns And Population Genetics Of Eastern Subterranean Termites Reticulitermes Flavipes In Urban Environment Of Nebraska, United States, Ah Ab Majid, Shripat T. Kamble, H. Chen

Department of Entomology: Faculty Publications

Reticulitermes flavipes (Kollar) has become the most destructive subterranean termite pest, on urban structures in Nebraska. In this study, we used seven microsatellite loci to infer the colony breeding system and population genetic structure among 20 infested urban structures in Nebraska. Our data revealed that 17 structures were infested by simple family colonies of R. flavipes, while, the remaining three were infested with mixed family colonies. The measure of population differentiation, FCT value (0.459) indicated that all the 20 urban colonies (10 - 410 km apart) represented pronounced levels of genetic differentiation. The Mantel test disclosed a weak and …

Rna Interference Of The Muscle Actin Gene In Bed Bugs: Exploring Injection Versus Topical Application For Dsrna Delivery, Sanjay Basnet, Shripat T. Kamble

Department of Entomology: Faculty Publications

Bed bugs are one the most troublesome household pests that feed primarily on human blood. RNA interference (RNAi) is currently being pursued as a potential tool for insect population management and has shown efficacy against some phytophagous insects. We evaluated the different techniques to deliver dsRNA specific to bed bug muscle actin (dsactin) into bed bugs. Initially, stability of dsRNA in human blood was studied to evaluate the feasibility of feeding method. Adult bed bugs were injected with dsRNA between last thoracic segment and first abdominal segment on the ventral side, with a dose of 0.2 μg dsactin …

In-Hive Acaricides Alter Biochemical And Morphological Indicators Of Honey Bee Nutrition, Immunity, And Development, Alison M. Reeves, Scott T. O'Neal, Richard D. Fell, Carlyle C. Brewster, Troy D. Anderson

Department of Entomology: Faculty Publications

The honey bee is a widely managed crop pollinator that provides the agricultural industry with the sustainability and economic viability needed to satisfy the food and fiber needs of our society. Excessive exposure to apicultural pesticides is one of many factors that has been implicated in the reduced number of managed bee colonies available for crop pollination services. The goal of this study was to assess the impact of exposure to commonly used, beekeeper-applied apicultural acaricides on established biochemical indicators of bee nutrition and immunity, as well as morphological indicators of growth and development. The results described here demonstrate that …

Host Plants Of Spodoptera Frugiperda (Lepidoptera: Noctuidae) In The Americas, Débora Goulart Montezano, Alexandre Specht, Daniel Ricardo Sosa-Gómez, Vânia F. Roque-Specht, José Carlos Sousa-Silva, Silvana L. Vieira De Paula Moraes, Julie A. Peterson, Thomas E. Hunt

Department of Entomology: Faculty Publications

The fall armyworm, Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae), is the most important noctuid pest in the Americas and has recently become an invasive pest in Africa. A detailed record of S. frugiperda’s host plants is essential to better understand the biology and ecology of this pest, conduct future studies, and develop Integrated Pest Management programmes. In this study, we collected and systematically arranged the fragmented bibliographic information on S. frugiperda feeding records. Furthermore, we registered new records of host plants for S. frugiperda based on eight years of surveys in Brazil. The literature …

Evaluation Of Foliar Insecticides For The Control Of Western Bean Cutworm In Field Corn, 2017, Katharine A. Swoboda-Bhattarai, Grace E. Hirzel, Julie A. Peterson

Department of Entomology: Faculty Publications

The objective of this field trial was to evaluate the efficacy of a single application of foliar insecticides at preventing feeding damage by the western bean cutworm (WBC), an important pest of corn and dry beans. Direct feeding by WBC larvae on developing kernels in the ear can result in yield loss, whereas WBC infestation can also lead to secondary fungal infections. WBC has undergone a rapid range expansion into the eastern Corn Belt within the last 16 yr. This study was conducted within the historic range of WBC, at the University of Nebraska-Lincoln’s Henry J. Stumpf International Wheat Center …

Z B Mayo, Jr. Obituary, Robert J. Wright, Tiffany Heng-Moss, Blair Siegfried

Department of Entomology: Faculty Publications

Emeritus Professor of Entomology at University of Nebraska–Lincoln, Dr. Z B Mayo, Jr., passed away on 25 March 2018 in Lincoln, Nebraska.

Employed at the University of Nebraska– Lincoln (UNL) from 1972 to 2010, he had a distinguished career as a researcher, teacher, mentor, and administrator. From 1972 to 1985, he conducted research on insects associated with corn production in Nebraska, with a major emphasis placed on the biology and control of corn rootworms. He and his students contributed significantly to our knowledge of corn rootworm biology and management. In 1983, he took a professional development leave and worked at …

Resistance In Rice To Tibraca Limbativentris (Hemiptera: Pentatomidae) Influenced By Plant Silicon Content, Lincoln Luis França, Cássio Antonio Dierings, André Cirilo De Sousa Almeida, Marcio Da Silva Araújo, Elvis Arden Heinrichs, Anderson Rodrigo Da Silva, José Alexandre Freitas Barrigossi, Flávio Gonçalves De Jesus

Department of Entomology: Faculty Publications

Tibraca limbativentris Stål (Hemiptera: Pentatomidae) is a major pest causing economic losses to rice cultivation in Brazil. The objectives of this work were to assess different sources of silicon in the induction of plant resistance, to examine the interaction of rice varieties with silicon-based resistance, and to determine the effects of varieties and silicon on stink bug biology. The interaction of rice cultivars BRS Esmeralda, IRGA 429 (widely cultivated in Brazil), and Canela de Ferro (a resistant cultivar), and 2 sources of silicon (K silicate, and Ca + Mg silicate) were studied. Plant parameters measured were percentage of damaged stems, …

Symposium Paper Building Bridges Between Disciplines For Sustainable Crop Protection, Rangaswamy Muniappan, E. A. Heinrichs

Department of Entomology: Faculty Publications

Plant protection is an interdisciplinary area that historically involved agronomy, plant breeding, entomology, plant pathology, statistics, and weed science. After World War II, pesticide development took precedence and chemistry, physics, and engineering were favored. This led to excessive use of synthetic pesticides, causing pest resistance to insecticides, contamination of ecosystems and undesirable health effects. As a result, the integrated pest management concept was developed in the 1960s. This expanded into new disciplines, such as mathematics, computer science, meteorology, and aeronautics to forecast pest occurrence and dispersal. It also included molecular biology and microbiology to produce biological control agents and GMOs …

Science At Engineer Cantonment, Hugh H. Genoways, Brett C. Ratcliffe, Carl R. Falk, Thomas E. Labedz, Paul R. Picha, John R. Bozell

University of Nebraska State Museum: Mammalogy Papers

Conclusions

It is our contention that Thomas Say, Titian Peale, Edwin James, and their colleagues of the Stephen Long Expedition of 1819–1820 were heavily engaged in scientific research, which took the form of the first biodiversity inventory undertaken in the United States. This accomplishment has been overlooked both by biologists and historians, but it should rank among the most significant accomplishments of the expedition. The results of this inventory continue to inform us today about environmental, faunal, and floral changes along the Missouri River in an area that is known to be an ecotone between the deciduous forests of the …

New Tribal Placement And Review Of Parapucaya Prell And Pucaya Ohaus (Coleoptera, Scarabaeidae, Dynastinae), Aura Paucar-Cabrera, Matthew Robert Moore

Department of Entomology: Faculty Publications

The dynastine scarab genera Parapucaya Prell and Pucaya Ohaus have been historically classified in Pentodontini; however, that tribal classification is not supported under the current tribal circumscriptions. A discussion justifying the transfer of the genera Parapucaya and Pucaya from Pentodontini into Cyclocephalini is presented. This research is based on morphological observations (mandible shape and wing characters among others) and molecular data (genes 28S, COI, and 16S/ND1). A review of both genera is included, providing descriptions, diagnoses, distribution data, illustrations, and keys to species. A revised key to the world genera of Cyclocephalini is also included.

Los escarabajos de los géneros …

Gene Silencing In Tribolium Castaneum As A Tool For The Targeted Identification Of Candidate Rnai Targets In Crop Pests, Eileen Knorr, Elane Fishilevich, Linda Tenbusch, Meghan L. F. Frey, Murugesan Rangasamy, Andre Billion, Sarah E. Worden, Premchand Gandra, Kanika Arora, Wendy Lo, Greg Schulenberg, Pablo Valverde-Garcia, Andreas Vilcinskas, Kenneth E. Narva

Department of Entomology: Faculty Publications

RNAi shows potential as an agricultural technology for insect control, yet, a relatively low number of robust lethal RNAi targets have been demonstrated to control insects of agricultural interest. In the current study, a selection of lethal RNAi target genes from the iBeetle (Tribolium castaneum) screen were used to demonstrate efficacy of orthologous targets in the economically important coleopteran pests Diabrotica virgifera virgifera and Meligethes aeneus. Transcript orthologs of 50 selected genes were analyzed in D. v. virgifera diet-based RNAi bioassays; 21 of these RNAi targets showed mortality and 36 showed growth inhibition. Low dose injection- and …

Clathrin-Dependent Endocytosis Is Associated With Rnai Response In The Western Corn Rootworm, Diabrotica Virgifera Virgifera Leconte, Daniele H. Pinheiro, Ana M. Velez, Elane Fishilevich, Haichuan Wang, Newton P. Carneiro, Arnubio Valencia-Jimenez, Fernando H. Valicente, Kenneth E. Narva, Blair D. Siegfried

Department of Entomology: Faculty Publications

The cellular uptake of dsRNA after dietary exposure is critical for RNAi efficiency; however, the mechanism of its uptake in many insects remains to be understood. In this study, we evaluated the roles of the endocytic pathway genes Clathrin heavy chain (Chc), Clathrin adaptor protein AP50, ADP ribosylation factor-like 1 (Arf72A), Vacuolar H⁺ ATPase 16 kDa subunit (Vha16), and small GTPase Rab7 and putative sid-1-like genes (silA and silC) in RNAi response in western corn rootworm (WCR) using a two-stage dsRNA exposure bioassay. Silencing of Chc, Vha16, and AP50 led …

The Mysteries Of Insect Rnai: A Focus On Dsrna Uptake And Transport, Ana Maria Vélez, Elane Fishilevich

Department of Entomology: Faculty Publications

RNA interference (RNAi) is becoming a practical tool to control insect pests. Many mysteries of how double-stranded RNA (dsRNA) is transported into, within, and between cells to generate an efficient RNAi response in insects are still to be unraveled. This review provides an overview of the evidence that supports a key role of endocytosis in the uptake of dsRNA on both cellular and tissue levels. Additionally, other components of cellular membrane transport and their impact on the efficiency of RNAi in insects are explored. It is now evident that the membrane transport and potentially dsRNA release from the endosome may …

Rapid And Persistent Rnai Response In Western Corn Rootworm Adults, Ke Wu, Caitlin E. Taylor, Elane Fishilevich, Kenneth E. Narva, Blair Siegfried

Department of Entomology: Faculty Publications

RNA interference (RNAi) has proven effective for controlling pest insects such as western corn rootworm (WCR), Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae). Previous studies have shown that WCR adults display a robust RNAi response to orally-administered double-stranded RNA (dsRNA). However, it is unclear how quickly the response occurs after ingestion or how long RNAi effect lasts after WCR stop ingesting diet containing dsRNA. In the current study, WCR adult females were provided with diet treated with dsRNAs of Laccase 2 and Argonaute 2, two nonessential genes, for 8 days. RNAi response in WCR females commenced as early as 10 h …