Terrestrial and Aquatic Ecology Commons^™

Chronic Toxicity Testing In Mining Influenced Streams Of West Virginia, Leah J. Bitzer

Theses, Dissertations and Capstones

Whole effluent toxicity (WET) tests have become a common tool in the evaluation of effluent for discharge acceptability. In this study, four years of toxicity data from 119 sampling locations were analyzed to determine relationships with ions and conductivity as indicators of toxicity. West Virginia Stream Condition Index (WVSCI) scores were also examined to evaluate correlations between stream scores, conductivity, and IC25 endpoints from toxicity results. Conductivity was not an indicator of toxicity in the range of conductivities tested. Streams dominated by mining effluent sometimes exhibited toxicity to Ceriodaphnia dubia; however, toxicity was not found to be related to ionic …

Plesiosaur Body Shape And Its Impact On Hydrodynamic Properties, Courtney D. Richards

Theses, Dissertations and Capstones

Despite the variability of cross-sectional body shape within Plesiosauria, its impact on plesiosaur buoyancy and stability has never been investigated. This study focused on Tatenectes, Cryptoclidus, and Muraenosaurus due to their variable body morphologies. Reconstructions were created based on measurements and photographs from fossil remains. The ability of computer models, based upon the reconstructions, to reach equilibrium after submersion, sink via lung deflation, and recover from a lateral roll was tested. For the computer models, Muraenosaurus was replaced with Thalassomedon, which had a similar morphology. Cryptoclidus and Thalassomedon recovered from submersion faster than Tatenectes. All models achieved negative buoyancy with …

The Little Coal River Improvement Project, West Virginia: An Initial Study Of Sediment, Bacteria And Benthic Macroinvertebrates, Emily Vargo

Theses, Dissertations and Capstones

The Little Coal River, located in southern West Virginia, has undergone many changes over the last few years in an effort to reverse the negative effects of various point source and non-point source pollutions. Marshall University, in cooperation with the West Virginia Department of Environmental Protection, Army Corp of Engineers, and the Coal River Group, has designed and implemented a plan for rehabilitation known as the Little Coal Improvement Project. Several improvement structures were strategically installed within the Little Coal River during the summer of 2007. The functions of these structures include sediment flushing and overall habitat improvement. Monitoring these …

Phylogenetic Analysis Of Blacknose Dace (Rhinichthys) In West Virginia Streams, Samantha Taylor

Theses, Dissertations and Capstones

Blacknose dace (Rhinichthys) are one of the most common cyprinid fishes in eastern North America. They also have been a topic of debate for over 30 years because morphology-based systematics has failed to clearly define their taxa. Taxonomists classify the complex into two species and one subspecies: the eastern form, R. atratulus atratulus; and the western form R. obtusus obtusus, and southern form R. obtusus meleagris. This research uses the mitochondrial cytochrome b gene and genomic RAG 2 gene in a phylogenetic analysis to help clarify species relations according to differences between each current species. Maps have been created to …

A Virtual Phytosaur Endocast And Its Implications For Sensory System Evolution In Archosaurs, Waymon Holloway

Theses, Dissertations and Capstones

Due to the overall morphological similarities between the Triassic archosaurs of the order Phytosauria and extant crocodilians, most studies have assumed that the two shared similar lifestyles. Many studies involving phytosaurs have focused on the external cranial morphology of various taxa. Internal cranial anatomy has received relatively little attention. As a result, comparative morphology studies of the braincases interior, or endocast, of phytosaurs are an area of potential exploration. Just as modern medical Xray computed tomography (CT) can be used to create three-dimensional images of internal structures of living subjects, such technology offers a non-invasive means of studying the internal …

The Natural History & Distribution Of Riverine Turtles In West Virginia, Linh Diem Phu

Theses, Dissertations and Capstones

Turtles are unique evolutionary marvels that evolved from amphibians and developed their protective shelled form more than 200 million years ago. In West Virginia, there are 10 native species of turtles, 9 of which are aquatic. Most of these aquatic turtles feed on carrion and dead plant matter, in the water and essentially "clean" our water systems. Turtles are long-lived animals with sensitive life stages that can serve as both long-term and short-term bioindicators of environmental health. With the increase in commercial trade, habitat fragmentation, degradation, destruction, there has been a marked decline in turtle species. The need for well …

The Coal River Basin: A 2009 Water Budget Study, R. Dale Biller

Theses, Dissertations and Capstones

The Coal River Watershed covers an area of some 890 square miles in southern West Virginia of which 863 square miles was investigated in this study. Both online and field data were collected over period from January 13, 2009 to January 13, 2010. The basin was studied as a closed system having an input, which was precipitation, and outputs, rainfall interception, evapotranspiration, and stream discharge that was separated into base flow and overland flow.

The effective rainfall of the watershed was calculated using the Thiessen polygon method to be 42 inches for the year, 48% of that water discharged by …

Dopamine Modulates The Lateral Giant Neuron And Serotonergic Facilitation In Crayfish, Joshua Scott Titlow

Theses, Dissertations and Capstones

The lateral giant (LG) neural circuit is a model system used to study the function of individual neurons. The LG circuit is part of a tail flip escape reflex that is a defensive behavior for crayfish. This thesis begins by addressing the effects of dopamine (DA), a neurotransmitter involved in normal and abnormal behaviors of most animals. Here it is shown that dopamine decreases the excitability of the LG neuron, a trigger for the escape reflex. An electrophysiology protocol was used to mimic sensory input to the LG neuron. Stimulating a sensory nerve in the last ganglion with an electrode …

Determining The Distribution Of Antibiotic Resistant And Fecal Indicator Bacteria In The Ohio River, Caitlin Nicole Swecker

Theses, Dissertations and Capstones

The Ohio River extends 981 miles from Pittsburgh, PA to Cairo, Ill providing drinking water to over three million people, a natural habitat for aquatic life, a public recreation resource, a major transportation route, and a source of water for industry. The Guyandotte River is a highly impacted tributary emptying into the Ohio River in Huntington, WV. The objectives of this study were to determine if sediment load is correlated to the number of antibiotic resistant bacteria (ARB) and determine if a single surface sample is a sufficient representative measurement of ARB populations in a large river. In 2007, subsurface …

Distribution Of Woodland Salamanders Of The Valley And Ridge In West Virginia, H. Reid Downer

Theses, Dissertations and Capstones

Two terrestrial woodland salamanders of the genus Plethodon, P. punctatus and P. virginia, are endemic to the Valley and Ridge Physiographic Province in West Virginia and Virginia and occupy limited geographic ranges. Two common and widespread species, P. cylindraceus and P. cinereus, also inhabit this region. To evaluate the distribution and habitat of these terrestrial salamanders I surveyed wooded ridges and slopes throughout the geographic range of the two endemic species by conducting daytime cover object searches and nocturnal visual encounter surveys. From March through November 2008, I recorded the presence of 321 woodland salamanders at 91 sites. I used …

Terrestrial Habitat Use Of Marbled Salamanders Ambystoma Opacum: A Site Specific Approach, Frank Piccininni

Theses, Dissertations and Capstones

Habitat destruction, fragmentation and degradation have contributed heavily to the decline of amphibian populations such as Marbled Salamanders (Ambystoma opacum). Often, ambystomatid water resources are conserved without consideration for the equally important terrestrial habitat. This is partly due to a lack of information regarding the relationship between ecological succession, plant community composition, microhabitat and salamander abundance. Three sampling transects consisting of drift fence arrays, vegetation assessments and microhabitat surveys were extended 100 m into the terrestrial habitat surrounding a seasonal wetland at Beech Fork State Park in Wayne County, West Virginia. Principal components analysis was used to identify …

Turtle Assemblages In The Eastern Panhandle Of West Virginia With An Emphasis On Pseudemys Rubriventris (Leconte), Ashley Renea Fisher

Theses, Dissertations and Capstones

Little is known about turtle assemblages in West Virginia, especially in the eastern panhandle. The only published work on Pseudemys rubriventris (Le Conte) in the state is by Green and Pauley (1987). Pseudemys rubriventris are considered an S2 species by the West Virginia Division of Natural Resources which means they are considered rare and imperiled in the state (WVDNR, Wildlife Diversity Program). Their range includes the Coastal Plains rivers of the mid-Atlantic region, from northern North Carolina to central New Jersey (Ernst et al. 1994). In West Virginia, they inhabit the Ridge and Valley physiographic province, which is the only …

A Study Of The Genus Desmognathus In West Virginia, With Emphasis On Desmognathus Welteri, The Black Mountain Salamander, Tristan Bond

Theses, Dissertations and Capstones

The Black Mountain Salamander, Desmognathus welteri, is listed as S2 by the WVDNR. Given the threat of extirpation, this species is in need of studies to facilitate a conservation effort. The following studies were completed as part of this work: Comparative morphology of the genus Desmognathus in West Virginia; Distribution of D. welteri in West Virginia; Habitat partitioning of Desmognathine salamanders; and Phenology of Desmognathus spp. Ratios of morphometrics of the tail were found to be most useful for distinguishing Desmognathus spp. D. welteri was found in three of 22 streams searched, meaning there are now 21 documented occurrences of …

The Natural History And Thermal Ecology Of A Population Of Spotted Turtles (Clemmys Guttata) And Wood Turtles (Glyptemys Insculpta) In West Virginia, Ariana N. Breisch

Theses, Dissertations and Capstones

An ecological study was conducted from 19 March 2001 to 2 April 2003 on a population of Spotted Turtles (Clemmys guttata) and Wood Turtles (Glyptemys insculpta) in West Virginia. Live-trapping, mark-recapture, radio telemetry and temperature data logging techniques were used to gather information on morphometrics, capture success, movements, home range, behavior, and thermal regime during activity and over-wintering seasons. Twenty-one Spotted Turtles were captured 260 times. Population structure was 42.9% juvenile, 38.1% male, and 19.0% female. Mean home range was 0.52 ha. Fifty Wood Turtles were captured 230 times. Juveniles were 36%, and males and females were 32% each. Mean …

Constructed Ponds As Mitigated Habitat For The Wood Frog (Rana Sylvatica Leconte) And The Spotted Salamander (Ambystoma Maculatum Shaw) In West Virginia, Celeste Dawn Good

Theses, Dissertations and Capstones

Many forest dwelling amphibians depend upon aquatic breeding habitats, making them susceptible to habitat changes. To determine if amphibian use of temporary pools occurred, 9 ponds were constructed in 3 forested areas on the MeadWestvaco Wildlife and Ecosystem Research Forest. Studies were conducted in 6 ponds during 2004, and all 9 in 2005 using drift fences. Trapped amphibians were measured and given a pond specific mark with visible implant elastomers. A significant difference was found between low and high elevation sites for juvenile R. sylvatica snout-to-vent length. No significant differences were found for soil, air or water temperatures between sites …

Survey Of The Great Kanawha River, West Virginia, For Virulence Related Gene Markers Stx1, Stx2, And Eaea, Christina Carole Johnson

Theses, Dissertations and Capstones

Microbial surveying using antibiotic resistant bacteria, fecal coliforms, and virulence genes is an approach not previously tested on the Great Kanawha River. Research objectives were to test antibiotic resistant and fecal coliform bacteria as bioindicators of water quality, and develop a multiplex- polymerase chain reaction (mPCR) system for identification of stx1, stx2, and eaeA DNA sequences in isolated fecal coliforms (FC). Fecal indicator populations and antibiotic resistant populations were demonstrated to be independent. Bacterial populations were assigned impact score(s) (IS) values for each sample site based on data set percent ranks. Population scores were summed per sample site to generate …

Habitat Analysis By Hierarchical Scheme And Stream Geomorphology, James B. Spence

Theses, Dissertations and Capstones

A study was undertaken to classify eight stream reaches in the North Branch of the Potomac River watershed and determine if geomorphologic differences influenced the availability of fish habitat structure and fish density. Stream reaches were classified using Rosgen Level II (1996) methods, and fish habitat was determined using Hydraulic Channel Unit (HCU) classification based on a method modified from Bisson et al. (1982). Other habitat variables were also studied such as stream shading and physical habitat based on the Rapid Bioassessment Protocol (Barbour et al. 1999). Despite the differences in HCU density between sites, HCU density did not influence …

Spatial Heterogeneity Of Soil Nutrients, Nitrogen Dynamics, And Vegetation In A 3rd Order Stream Floodplain In Southwestern West Virginia, David Allen Dick

Theses, Dissertations and Capstones

Soil processes often exhibit spatial heterogeneity within ecosystems and this heterogeneity may influence community structure. This study was conducted to determine spatial patterns and variability of soil nutrients and plant communities within different vegetation types in a stream floodplain in southwestern West Virginia. One 5-m × 5-m site was established in each of three vegetation/drainage types: pasture (PA), old field (OF), and wetland scar (SC). Pasture and SC sites were located ~25 m apart on flat bottomland; the OF site was located on a moderate slope 6.3 m above bottomland, ~200 m from PA and SC. A 10-m × 1-m …

Comparisons In Morphology, Reproductive Status, And Feeding Ecology Of Plethodon Cinereus At High And Low Elevations In West Virginia, Mizuki Takahashi

Theses, Dissertations and Capstones

To develop effects of elevation on morphological features, reproductive status, and feeding ecology of Plethodon cinereus in West Virginia, overall comparisons between high (>4000 ft) and low (<1260 ft) elevation populations were made. Adult P. cinereus from high elevations were smaller in SVL (female: p=0.003, male: p<0.001), but stored larger amounts of fat (female: p=0.041, male: p=0.006) in their tails than fromlow elevations. Larger amounts of tail fat could be an adaptation to harsh environments at high elevations. It was determined that in West Virginia, where as females at low elevations oviposit annually, females at high elevations oviposit biennially. Stomach content analysis revealed that salamanders at high elevations were confronted by food shortage. Because of less prey availability and longer winters at high elevations, salamanders at high elevations attained the smaller body size than at low elevations and could not obtain sufficient energy in 1 year to yolk a clutch.

Effects Of Developmental Activities On Streamside Salamander Communities In Boone County, West Virginia, Mindy S. Hamilton

Theses, Dissertations and Capstones

A study was conducted from May 1998 through June 1999 in three streams associated with mountaintop mining and valley fills in Boone County, West Virginia. Streamside salamander communities were monitored in these streams, as well as in two relatively undisturbed reference streams in the region, using refugia bags, rock-on-rock methodology, and day surveys. The relative abundance of salamanders was compared between the streams in order to detect effects of the associated valley fills to downstream biota. Relative abundance was lower in two of three streams associated with valley fills as compared to the reference streams. However, there was no significant …

Natural Succession Of Benthic Populations In Constructed Sediment Ponds And Ditches In Southwestern West Virginia, Robin E. Dolin

Theses, Dissertations and Capstones

Strip mining causes major disturbances of the natural environment. One such disturbance is the creation of valley fills, which often fill in the headwaters of small streams. Runoff from these valley fills can cause heavy siltation as well as acid and heavy metal deposition downstream. One way to combat this problem is through the construction of sedimentation ponds, which slow down the flow of water so that sediment can settle out and water chemistry can be altered before the water is discharged into the stream. Since these ponds are, in effect, temporarily replacing small headwaters once present, the question is …

Factors Influencing Spatial Variability In Soil Nitrogen (N) Dynamics In N-Treated And Untreated Watersheds Of The Fernow Experimental Forest, West Virginia, Nikki Lenore Lyttle

Theses, Dissertations and Capstones

The central Appalachian region of the United States receives some of the highest inputs of nitrogen (N) due to acidic deposition in the nation. It is believed that these high could levels contribute to a decline in forest soils within the next 50 to 70 yrs. This study examines factors that influence spatial variability in N-treated and untreated watersheds of the Fernow Experimental Forest, Parsons, West Virginia. Within each of the two watersheds [WS4 untreated control, > 100 yr.; WS3 N-treated, acidified, clear cut, ~ 31 yr.], two 0.04 ha plots, one high N and one low N, were selected for …

Computer Simulation For Feature Extraction In Pattern Recognition And Geobiophysical Modeling In Mountaineous Wetland Delineation, Natalie Ripsher

Theses, Dissertations and Capstones

Delineation of wetlands is an important aspect of protecting of these ecologically diverse and biocomplex habitats. Selected image-processing techniques are applied to remotely sensed imagery of Canaan Valley, in order to determine the best method of extracting wetland features for habitat delineation. Remotely sensed data from April 1997 and September 1999 were compared to determine the accuracy of feature extraction, and the effect of seasonal and long term climate change on wetland delineation. Pattern recognition for feature extraction was used to identify vegetation associations and changes in habitats. Computer simulation of the infrared band was performed using ER Mapper so …

Habitat Utilization Of Fish Species On The Ohio River: Preliminary Development Of A Multi-Metric Habitat Index, James P. Hawkes

Theses, Dissertations and Capstones

Development of a habitat index requires an understanding of the longitudinal distribution of habitat, fish assemblages, and how the two interact. Because of the complexity and size of the Ohio River, this understanding has not been reached. Habitat analysis has long been considered, and is essential, in assigning impaired and reference condition of habitat quality. The Ohio River is diverse in the distribution of its habitat within pool and river-wide. An Analysis of Variance (ANOVA) was used to analyze these distributions. Within pool assessment of % habitat composition revealed woody cover and vegetation types were significantly greater in the lowest …

Distribution Of The Sucker Family (Pisces: Catostomidae) In The Ohio River, Tara L. Rose

Theses, Dissertations and Capstones

The family Catostomidae represents a major component of the fish fauna in the Ohio River. Because of their abundance, large biomass, and susceptibility to environmentally-induced anomalies, suckers are expected to significantly influence the Ohio River Fish Index (ORFIn), a biological index being developed by the Ohio River Valley Water Sanitation Commission (ORSANCO) for large river systems. To correctly interpret information obtained from ORFIn, the impact of suckers upon the index must be thoroughly understood, including relative importance and uneven spatial distribution of Ohio River suckers. In this study, suckers represented 7.1 percent of the total fish captured and 36.6 percent …

Ecology And Natural History Of Rana Clamitans Melanota In West Virginia, Alison M. Rogers

Theses, Dissertations and Capstones

Fourteen study sites were sampled with aquatic funnel traps and D-frame net sweeps in the plateau and mountain region of West Virginia to determine differences in larval period. Larval stage class abundance and larval total length and mass measurements were recorded from February to December 1997. Larval stage classes 25 and 26, the first free living stages, were absent from the plateau region from February to April, but were present in the mountain region. Pre-transformation larval stages, 40 through 44, were found from May through October, and peaked in July. Significant relationships were found with simple linear regression analysis between …

Benthic Biodiversity And Physico-Chemical Parameters Of Acid Mine Drainage, Acid Impacted And Nonimpacted Streams, Stephen D. Greene

Theses, Dissertations and Capstones

South Fork of Sand Lick Creek, Logan County, West Virginia, drains an abandoned coal strip mine which had exploited Pottsville series coalbeds (Pennsylvania strata). These strata outcrop throughout southwestern West Virginia. North Fork watershed is relatively unchanged, save a small roadcut throughout. South Fork benthic community had not recovered although mining activity had ceased about 20 years earlier. Benthic communities were analyzed with detrended correspondence analysis (DCA). Family Chironomidae predominated South Fork benthic community throughout the study. North Fork’s benthic community had as major contributors acid resistant caddisfly family Hydropsychidae, mayfly family Baetidae, and stonefly families Perlodidae and Nemouridae. Family …

Results In The Development Of A Macroinvertebrate Index For The Ohio River, Matthew S. Wooten

Theses, Dissertations and Capstones

Aquatic macroinvertebrates are utilized widely as indicators of water quality. Many indices are in use today that provide a means of applying a numeric value to the water quality of a particular stream. However, these indices have been designed for use on small streams and creeks. The primary objective of this study was to lay a foundation for the development of a marcroinvertebrate index to be used on the Ohio River. Secondarily, this study examined for longitudinal trends, trends in correlation with effluent location and the determination of least impacted or reference conditions on the Ohio River. Regression correlation analysis …

Reproductive Biology, Food Habits, And Forage Ratios Of The Candy Darter, Etheostoma Osburni, In The Cherry River, West Virginia, With An Analysis Of Mitochondrial Dna, Ann Elizabeth Schoolcraft

Theses, Dissertations and Capstones

Etheostoma osburni, the candy darter, is endemic to the lower New River drainage of West Virginia and Virginia and Gauley River drainage of West Virginia. As a result of its endemism and evidence of declining population numbers, the species has been given the status of Special Concern in both states. Candy darters occur in the Appalachian Plateau and Ridge and Valley provinces, and are influenced by different environmental selective pressures. In this study, candy darters were collected in the summer and fall of 1998 and spring of 1999 by backpack electroshocking for determination of their seasonal reproductive biology, food …

Life History, Ecology, And Low Ph Tolerance Of The Freshwater Prawn, Palaemonetes Kadiakensis Rathbun, In A Mitigated Wetland Of The Green Bottom Wildlife Management Area, West Virginia, Jennifer Ann Aderman

Theses, Dissertations and Capstones

An ecological life history study of the freshwater prawn Palaemonetes kadiakensis was conducted at the mitigated wetland of the Green Bottom Wildlife management Area (GBWMA). Monthly samples were collected from November 1996 through November 1997. In addition, weekly samples were collected from May through November for growth and reproductive analysis. Monthly samples yielded 520 prawns. Palaemonetes kadiakensis had a one-year life cycle, with all adults dying by September. Males averaged 23.82 mm (range = 14.15 to 36.75 mm) and females averaged 26.78mm (range = 15.06 to 38.60 mm) in length. Males averaged 0.087g (range = 0.014to 0.293 g) and females …