Terrestrial and Aquatic Ecology Commons^™

Bayesian Integrated Species Distribution Models For Hierarchical Resource Selection By A Soaring Bird, Ryo Ogawa, Guiming Wang, L. Wes Burger, Bronson Strickland, J. Brian Davis, Fred L. Cunningham

United States Department of Agriculture Wildlife Services: Staff Publications

Migratory birds exhibit seasonal geographic range (hereafter, range) dynamics during the annual cycle. Few studies have examined how migratory birds select their habitats for range occupancy at the species level and space use at the individual level simultaneously. We hypothesized that environmental variables directly related to fitness components would affect the range occupancy probabilities of migrants, whereas environment variables related to movements and flights would affect the space use intensities of migrants. We built Bayesian integrated species distribution models (ISDMs) to evaluate the effects of climate conditions, wind conditions, and landcover compositions on the seasonal range dynamics of American white …

Raccoon Density Estimation From Camera Traps For Raccoon Rabies Management, Amy J. Davis, Wesley C. Dixon, Richard B. Chipman, Amy T. Gilbert, Jacob E. Hill, James C. Beasley, Olin E. Rhodes Jr., Guha Dharmarajan

United States Department of Agriculture Wildlife Services: Staff Publications

Density estimation for unmarked animals is particularly challenging, yet density estimates are often necessary for effective wildlife management. Raccoons (Procyon lotor) are the primary terrestrial wildlife reservoir for Lyssavirus rabies within the United States. The raccoon rabies variant (RRVV) is actively managed at landscape scales using oral rabies vaccination (ORV) within the eastern United States. To effectively manage RRVV, it is important to know the density of raccoons to appropriately scale the density of ORV baits distributed on the landscape. We compared methods to estimate raccoon densities from camera‐trap data versus more intensive capture‐mark‐recapture (CMR) estimates across 2 …

Effect Of Walleye Fry Stocking On Future Gillnet Cpue, Alexander Barker

Journal of Earth and Life Science

Walleye Sanders vitreus for many states, is a key attractant of anglers from around North America. Due to immense popularity, management efforts have been conducted to maintain Walleye populations, especially in the form of stocking. The purpose of this study is to analyze the effect of Walleye fry stocking on gillnet catch per unit effort (CPUE). A total of 90 lakes were analyzed for this study within three defined regions of Minnesota, 30 from each region. The regions chosen for this study are the Minnesota Department of Natural Resources northern pike Esox lucius regulation regions which consist of the Southern, …

Landscape-Scale Epidemiological Dynamics Of Sars-Cov-2 In White-Tailed Deer, Joshua Hewitt, Grete Wilson-Henjum, Derek T. Collins, Timothy J. Linder, Julianna B. Lenoch, Jonathan D. Heale, Christopher A. Quintanal, Robert Pleszewski, Dillon S. Mcbride, Andrew S. Bowman, Jeffrey C. Chandler, Susan A. Shriner, Sarah N. Bevins, Dennis J. Kohler, Richard B. Chipman, Allen L. Gosser, David L. Bergman, Thomas J. Deliberto, Kim M. Pepin

United States Department of Agriculture Wildlife Services: Staff Publications

Understanding pathogen emergence in new host species is fundamental for developing prevention and response plans for human and animal health. We leveraged a large-scale surveillance dataset coordinated by United States Department of Agriculture, Animal and Plant Health Inspection Service and State Natural Resources Agencies to quantify the outbreak dynamics of SARS-CoV-2 in North American white-tailed deer (Odocoileus virginianus; WTD) throughout its range in the United States. Local epidemics in WTD were well approximated by a single-outbreak peak followed by fade out. Outbreaks peaked early in the northeast and mid- Atlantic. Local effective reproduction ratios of SARS-CoV-2 were between …

What Is Known, Unknown, And Needed To Be Known About Damage Caused By Wild Pigs, Kurt C. Vercauteren, Kim M. Pepin, Seth M. Cook, Sophie Mckee, Abigail Pagels, Keely J. Kohen, Ingrid A. Messner, Michael P. Glow, Nathan P. Snow

United States Department of Agriculture Wildlife Services: Staff Publications

Damage assessments provide evidence for initiating and evaluating management programs that protect natural resources and human livelihoods against invasive species. Wild pigs (Sus scrofa) cause extensive damage in their native and non-native ranges, but the extent of current damage and efficacy of management of the damage (i.e., population control of wild pigs, exclusion fencing, etc.) remains poorly described. We conducted a systematic review of physical damage caused by wild pigs to summarize what is known and identify knowledge gaps for damage assessment. Wild pig damage assessments have been overwhelmingly qualitative (84% of studies) and measured differently across studies, …

Tracking Sero-Molecular Trends Of Swine Brucellosis In Hawai'i And The Central Pacific, Thi Hai Au La, Ian A. Mcmillan, Prashant Dahal, Andrew H. Burger, Mahdi Belcaid, Darrin M. Phelps, Samuel M. Goldstein, Vienna R. Brown, Michael H. Norris

United States Department of Agriculture Wildlife Services: Staff Publications

Introduction

Brucellosis is a zoonotic disease of mammals caused by bacterial species of the Brucella genus. The reservoir for disease is typically mammals, with species of Brucella found infecting amphibians, bats, and marine mammals. Brucella spp. can pass directly to humans through contact with infected animals or their products. Brucella spp. can cause chronic debilitating infections in mammals, including humans, and is associated with spontaneous abortions in infected animals, causing reduced fecundity. In Hawai‘i, terrestrial species that could harbor Brucella spp. include swine, cattle, horses, and axis deer among others. The numerous feral swine in Hawai‘i are known to carry …

An Effective Method For Long-Term Control Of American Bullfrogs Syntopic With Special-Status Native Amphibians In California, Usa, Jeff A. Alvarez, Jeffery T. Wilcox

Human–Wildlife Interactions

American bullfrogs (Lithobates catesbeianus) are invasive in western North America and are well established in California, USA, where they are widespread. This invasive species has been implicated in the decrease of native amphibian populations and is believed to have contributed to the decline of threatened and endangered amphibians regionally. We utilized air rifles, tin alloy pellets, and 2 shooters to systematically control L. catesbeianus in both lentic and lotic habitat types within 2 counties in California. We visited sites monthly (April through November) for approximately 8 and 14 years to lethally target and remove L. catesbeianus …

Waterbird-Perceived Damage, Control Measures, And Economic Implications For Rice Production In The Brazilian Cerrado, Demetrio Luis Guadagnin, Alexandre Afonso Meyer, João Augusto Rossi Borges, Fernanda Maia Justo

Human–Wildlife Interactions

Waterbirds have the potential to inflict economic damage to crops. However, is rice (Oryza sativa) production in the Brazilian Cerrado significantly affected by waterbirds? This study aimed to assess the extent of waterbird damage on irrigated rice production in Central Brazil. We conducted surveys of waterbird populations in rice agroecosystem habitats throughout the cultivation cycle and analyzed the expenses associated with waterbird-induced yield losses and the control measures employed by farmers. The 2 most prevalent waterbird species observed were Dendrocygna viduatta and D. autumnalis, comprising 68.6% of all recorded waterbirds. These species were more frequently encountered and …

Genetic Tracking Of A Rabid Coyote (Canis Latrans) Detected Beyond A Rabies Enzootic Area In West Virginia, Usa, Matthew W. Hopken, Crystal Gigante, Amy T. Gilbert, Richard B. Chipman, Jordana D. Kirby, Rene Edgar Condori, Samuel Mills, Chelsea Hartley, John Forbes, Lisa Dettinger, Dongxiang Xia, Yu Li, Bridgett Vonholdt

United States Department of Agriculture Wildlife Services: Staff Publications

Wildlife translocation and cross-species transmission can impede control and elimination of emerging zoonotic diseases. Tracking the geographic origin of both host and virus (i.e., translocation versus local infection) may help determine the most effective response when high-risk cases of emerging pathogens are identified in wildlife. In May 2022, a coyote (Canis latrans) infected with the raccoon (Procyon lotor) rabies virus variant (RRV) was collected in Lewis County, West Virginia, USA, an area free from RRV. We applied host population genomics and RRV phylogenetic analyses to determine the most likely geographic origin of the rabid coyote. Coyote …

Estimation Of Probability Of Habitat Use Of Roosevelt Elk On The Olympic Peninsula, Vincent Michael Gugliotti

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Estimating the probability of habitat use for a particular species is crucial to the direct management and conservation of that species. Without knowledge of habitat preferences, managers cannot effectively focus efforts on vital resources or landscape types. However, modelling probability of habitat use can be done in several ways which leaves room for variation and uncertainty in the estimates produced by each method. This study is an examination of the variation between two estimates of probability of habitat use while focusing on a particular subspecies of elk that inhabits a unique ecosystem relative to other elk subspecies. I modeled elk …

Estimating Daily Growth Of Age-0 Channel Catfish In A Free-Flowing, Commercially Exploited Midwestern River, Kaleb B. Wood

Masters Theses

The larval stage in fishes is an important stage in the life history, as their survival dictates the ability of the population to sustain itself as an adult population. While there is a plethora of information available for juvenile and adult life stages of Channel Catfish (Ictalurus punctatus), very little is understood about their larval life stage. This study sought to determine overall density, growth rates, and size structure of cohorts of age-0 Channel Catfish during a study year on the Wabash River. In 2018, I sampled age-0 Channel Catfish using a benthic trawl from May to September. …

Examining Bigheaded Carp Ichthyoplankton Drift In Tributary And Mainstem River Systems Of Two Midwestern Rivers, Adam Landry

Masters Theses

The bigheaded carp invasion in North America continues to negatively impact ecosystems making it essential to understand their reproductive dynamics at various spatial scales. Previous studies have highlighted the suitability of tributaries of large rivers for bigheaded carp reproduction, and the importance of tributaries for ichthyoplankton drift to mainstem rivers. This study investigated patterns in tributary-mainstem ichthyoplankton drift dynamics in two invaded river systems of the Midwestern United States, with distinct hydrology, the Illinois and Wabash Rivers. From May-September 2021, I sampled ichthyoplankton from three tributaries and three mainstem sites in each river basin. I analyzed bigheaded carp egg and …

Can Edna Be Used To Locate Shawnee Hills Cavefish Forbesichthys Papilliferus?, Megan Brandt, Timothy Spier Phd, Gary Zeruth Phd

Murray State Theses and Dissertations

Traditional stream fish sampling methods, such as seining and electrofishing, are time consuming, expensive, and limited to short stretches of stream. However, the analysis of environmental DNA (eDNA) from water samples promises to be a quick, inexpensive method for detecting fish. The streams of the Land Between the Lakes Recreational Area (LBL) are unique, undisturbed streams which contain many interesting fish species for which little information exists. One such rare species is the Shawnee Hills Cavefish Forbesichthys papilliferus, which is known to exist in a few LBL streams. However, the complete distribution of the Shawnee Hills Cavefish in LBL …

Considerations And Applications Of Environmental Dna In Biodiversity Studies, Sarah Tomke

Theses and Dissertations--Forestry and Natural Resources

Environmental DNA (eDNA) sampling—the detection of species through genetic material shed into the environment—has become an efficient and reliable method for biodiversity assessments. The method has grown in popularity due to its non-invasive and non-destructive nature, and has revolutionized monitoring efforts for rare, elusive, and cryptic species for which traditional sampling approaches are often costly and ineffective. Hundreds of studies have utilized this technique to study species occupancy, delineate distributions, and assess relative community composition. Although eDNA sampling is an effective strategy for many conservation and ecological studies, recently, light has been shed on some of its potential drawbacks and …

Bird Predation On Coho Salmon (Oncorhynchus Kisutch) In Humboldt Bay, Colton J. Dixon

Cal Poly Humboldt theses and projects

Coho Salmon (Oncorhynchus kisutch) populations along the west coast of the United States are declining. In Humboldt County, located in northwestern California, Coho Salmon have been in decline since their federal listing as threatened in 1997, primarily due to habitat loss from logging, agriculture, and urban development. Although these anthropogenic factors caused salmon populations to decline, bird predation may also be hindering population recovery, as evidenced by the recovery of Passive Integrated Transponder (PIT) tags, originally implanted in Coho Salmon, from bird colonies in Humboldt Bay. This study examines predation by Double-crested Cormorants (Phalacrocorax auritus), Caspian …

Pond Characteristics That Influence Oviposition Site Selection By Two Pond-Breeding Amphibians, Northern Red-Legged Frog (Rana Aurora) And Northwestern Salamander (Ambystoma Gracile), Jessica Jones

Cal Poly Humboldt theses and projects

Pond-breeding amphibians connect aquatic and terrestrial habitats through their biphasic life cycle, and understanding pond characteristics that support oviposition sites for multiple species is important for amphibian conservation. Two common amphibians in the Pacific Northwest, Northern red-legged frog (Rana aurora) and Northwestern salamander (Ambystoma gracile) often oviposit in the same pond; however, prior studies have focused on pond use by one species or the other, but not both together. I surveyed pond and oviposition site characteristics of 26 ponds in Humboldt County, CA, 10 of which were used only by R. aurora and 16 of which …

Long Term Trends In Mortality Rates Of A Smallmouth Bass Population, Caleb D. Anderson

Journal of Earth and Life Science

Smallmouth bass Micropterus dolomieu are known as a popular sport fish for anglers. When a known smallmouth bass fishery appears to have a dwindling population, it raises questions about the population health. A common indicator of the health of the population is the mortality rate. By comparing mortality, conclusions can be made on whether smallmouth bass are dying at a different rate in the system over time. Therefore, the objective of this study was to see if there had been a significant change in smallmouth bass mortality over a span of thirty years in Round Lake (DOW:010204), Aitkin County, Minnesota. …

Relationship Between Northern Pike And Walleye Cpue In Minnesota Lakes, Simon Oslund

Journal of Earth and Life Science

Northern pike Esox lucius and walleye Sander vitreus are both top predators in freshwater systems. Due to their predatorial nature, high populations of one species can prohibit populations of the other species from thriving, specifically northern pike foraging on walleye. This information is tracked by the Minnesota Department of Natural Resources through catch per unit effort (CPUE). CPUE data was gathered for all lakes in Beltrami County, Minnesota, and a regression analysis was done to test for a relationship between pike and walleye. It was found that the relationship between northern pike and walleye CPUE was significant (P = 0.02). …

Treatment With The Immunocontraceptive Vaccine, Gonacon, Induces Temporary Fertility Control In Free-Ranging Prairie Dog Populations In Colorado, Usa, Aaron B. Shiels, Jackson Runte, Emily W. Ruell, Douglas C. Eckery, Gary W. Witmer, Daniel J. Salkeld

United States Department of Agriculture Wildlife Services: Staff Publications

Context

Prairie dogs (Cynomys spp.) are rodents enjoyed by some humans; yet, they cause crop and property damage, and carry zoonotic disease. Non-lethal control of prairie dogs is of interest in urban/suburban settings. The injectable immunocontraceptive vaccine GonaCon (active ingredient is gonadotropin-releasing hormone [GnRH]) has been shown to be effective at reducing fertility in equine and deer, and is a US EPA-registered vaccine for use in these ungulate species.

Aims

To conduct a replicated field study to test efficacy of GonaCon in black-tailed prairie dogs (C. ludovicianus), and if found to be efficacious, to help facilitate …

Comparison Of Ketamine-Xylazine, Butorphanol-Azaperone-Medetomidine, And Nalbuphine-Medetomidine-Azaperone For Raccoon (Procyon Lotor) Immobilization, Shylo R. Johnson, Christine K. Ellis, Chad Wickham, Molly R. Selleck, Amy T. Gilbert

United States Department of Agriculture Wildlife Services: Staff Publications

Raccoons (Procyon lotor) are frequently handled using chemical immobilization in North America for management and research. In a controlled environment, we compared three drug combinations: ketamine-xylazine (KX), butorphanol-azaperone-medetomidine (BAM), and nalbuphinemedetomidine- azaperone (NalMed-A) for raccoon immobilization. In crossover comparisons, raccoons received a mean of the following: 8.66 mg/kg ketamine and 1.74 mg/kg xylazine (0.104 mL/kg KX); 0.464 mg/kg butorphanol, 0.155 mg/kg azaperone, and 0.185 mg/kg medetomidine (0.017 mL/kg BAM); and 0.800 mg/kg nalbuphine, 0.200 mg/kg azaperone, and 0.200 mg/kg medetomidine (0.020 mL/kg NalMed-A). Induction time was shortest with KX (mean6SE, 10.060.7 min) and longest with NalMed-A (13.061.3 min). …

Using Prescribed Fire And Biosolids Applications As Grassland Management Tools: Do Wildlife Respond?, Brian E. Washburn, Michael J. Begier

United States Department of Agriculture Wildlife Services: Staff Publications

Prescribed burning is a management tool commonly used in forested ecosystems in the southeastern United States, but the influence of this method on grassland vegetation and wildlife in this geographic region is unknown. During 2009–2015, we conducted a study to determine if the application of prescribed burning and/or long-term biosolid applications alter plant communities and/or wildlife use of grassland areas at Marine Corps Air Station Cherry Point, Havelock, North Carolina. We monitored vegetation growth, measured plant community composition, and documented wildlife activity in four study plots for 3 years after the implementation of annual winter prescribed burns. Prescribed burning reduced …

Potential Landscape Connectivity For Invasive Wild Pigs (Sus Scrofa) Across The Northern Prairies Of North America, Corey J. Kramer, Melanie R. Boudreau, Ryan Powers, Kurt C. Vercauteren, Ryan S. Miller, Ryan K. Brook

United States Department of Agriculture Wildlife Services: Staff Publications

Understanding landscape scale connectivity is an essential component in the management of invasive species since connectivity facilitates their invasion potential. Invasive wild pigs (Sus scrofa) are among the most prolific invaders on the planet, causing billions of dollars in agricultural and environmental damage annually. Newly introduced to Canada in the 1980s, we examined wild pig invasion potential across the northern prairies from western Canada into the currently wild pig-free northern U.S. states. We used GPS collar data collected in the Canadian prairies to quantify resource selection and incorporated results into an electric circuit theory framework to evaluate potential …

Rapid Point-Of-Care Testing For Detection Of Antibodies To Toxoplasma Gondii In Black Vultures And Ring-Billed Gulls From Pennsylvania, Alexa Rosypal Von Dohlen, Sydney Randall, Jordyan Grays, Mark A. Dugo, Brian J. Hunt, Justin Brown, Kyle Van Why, Sawsan Ammar, Richard Gerhold

United States Department of Agriculture Wildlife Services: Staff Publications

Toxoplasma gondii is a zoonotic protozoan parasite that infects most warm-blooded animals, including birds. Scavenging birds are epidemiologically important hosts because they can serve as indicators of environmental T. gondii levels. A rapid point-of-care (POC) test that detects antibodies to T. gondii in humans is commercially available. In this research, we assessed the ability of the human POC test to detect anti–T. gondii antibodies in 106 black vultures (Coragyps atratus) and 23 ring-billed gulls (Larus delawarensis) from Pennsylvania, USA. Serum samples were tested with the POC test and compared to the modified …

Low Secondary Risks For Turkey Vultures From A Sodium Nitrite Toxic Bait For Wild Pigs, Justin A. Foster, John C. Kinsey, Michael J. Lavelle, Kurt C. Vercauteren, Nathan P. Snow

United States Department of Agriculture Wildlife Services: Staff Publications

An acute and orally‐delivered toxic bait for wild pigs (Sus scrofa) containing sodium nitrite (SN) is being developed and tested for potential registration in the U.S. We evaluated oral toxicity of SN and risk of secondary consumption in a common avian scavenger, the turkey vulture (Carthartes aura), by 1) conducting an acute oral toxicity test (LD₅₀) for turkey vultures by oral gavage, and 2) allowing turkey vultures to feed on whole carcasses and digestive tracts of wild pigs that died from consuming SN‐laden bait. Captive tests were conducted during fall 2016 and spring 2017 in the …

A Method For Characterizing Disease Emergence Curves From Paired Pathogen Detection And Serology Data, Joshua Hewitt, Grete Wilson-Henjum, Derek T. Collins, Jourdan M. Ringenberg, Christopher A. Quintanal, Robert Pleszewski, Jeffrey C. Chandler, Thomas J. Deliberto, Kim M. Pepin

United States Department of Agriculture Wildlife Services: Staff Publications

1. Wildlife disease surveillance programs and research studies track infection and identify risk factors for wild populations, humans and agriculture. Often, several types of samples are collected from individuals to provide more complete information about an animal's infection history. Methods that jointly analyse multiple data streams to study disease emergence and drivers of infection via epidemiological process models remain underdeveloped. Joint-analysis methods can more thoroughly analyse all available data, more precisely quantifying epidemic processes, outbreak status, and risks.

2. We contribute a paired data modelling approach that analyses multiple samples from individuals. We use ‘characterization maps’ to link paired data …

Torque Teno Sus Virus 1: A Potential Surrogate Pathogen To Study Pig-Transmitted Transboundary Animal Diseases, Xiaolong Li, Brandon M. Parker, Raoul K. Boughton, James C. Beasley, Timothy J. Smyser, James D. Austin, Kim M. Pepin, Ryan S. Miller, Kurt C. Vercauteren, Samantha M. Wisely

United States Department of Agriculture Wildlife Services: Staff Publications

Understanding the epidemiology and transmission dynamics of transboundary animal diseases (TADs) among wild pigs (Sus scrofa) will aid in preventing the introduction or containment of TADs among wild populations. Given the challenges associated with studying TADs in free-ranging populations, a surrogate pathogen system may predict how pathogens may circulate and be maintained within wild free-ranging swine populations, how they may spill over into domestic populations, and how management actions may impact transmission. We assessed the suitability of Torque teno sus virus 1 (TTSuV1) to serve as a surrogate pathogen for molecular epidemiological studies in wild pigs by investigating …

Common Grackle (Quiscalus Quiscula) Predation Of A Great Lakes Piping Plover (Charadrius Melodus) Nest And Its Conservation Implications, Jade R. Arneson, Dennis A. Peloquin, Thomas G. Prestby, Sarah P. Saunders

United States Department of Agriculture Wildlife Services: Staff Publications

Common grackle (Quiscalus quiscula) was observed consuming eggs from an exclosed (protected) Great Lakes piping plover (Charadrius melodus) nest in the lower bay of Green Bay, Lake Michigan in Wisconsin, USA during June 2023. The observation, captured by a trail camera, is the first photographic documentation of grackle predation in any of the three piping plover breeding populations. This behavior adds common grackle to the suite of egg predators and has critical implications for this shorebird species, particularly in the Great Lakes region where piping plover is listed as federally endangered and common grackles are relatively …

Trophic Interactions Of Sympatric Cyprinodontiform Fishes Inhabiting Louisiana Saltmarshes, Ryan Heines

Dissertations, Master's Theses and Master's Reports

Resident saltmarsh nekton, namely Cyprinodontiform fishes, play key ecological roles in supporting terrestrial and aquatic food webs. These fishes are highly abundant across the saltmarsh landscape of coastal Louisiana and have evolved to tolerate the highly variable environmental conditions that are characteristic of these habitats. The objective of this research is to consider mechanisms enabling this diverse community of fishes to cooccur in the spatially limited saltmarsh habitats of coastal Louisiana. In Chapter 2, bulk stable isotope ratios of carbon (δ13C), sulfur (δ34S) and nitrogen (δ15N) were used to quantify the intra- and inter-specific resource use of six sympatric Cyprinodontiform …

Natural Selection On Feralization Genes Contributed To The Invasive Spread Of Wild Pigs Throughout The United States, Niek W. G. Barmentlo, Patrick G. Meirmans, William H. Stiver, Joseph G. Yarkovich, Blake E. Mccann, Antoinette J. Piaggio, Dominic Wright, Timothy J. Smyser, Mirte Bosse

United States Department of Agriculture Wildlife Services: Staff Publications

Despite a long presence in the contiguous United States (US), the distribution of invasive wild pigs (Sus scrofa × domesticus) has expanded rapidly since the 1980s, suggesting a more recent evolutionary shift towards greater invasiveness. Contemporary populations of wild pigs represent exoferal hybrid descendants of domestic pigs and European wild boar, with such hybridization expected to enrich genetic diversity and increase the adaptive potential of populations. Our objective was to characterize how genetic enrichment through hybridization increases the invasiveness of populations by identifying signals of selection and the ancestral origins of selected loci. Our study focused on invasive …

Influence Of Habitat And Baiting Strategy On Oral Rabies Vaccine Bait Uptake By Raccoons In The Southeastern United States, Jacob E. Hill, Madison L. Miller, Richard B. Chipman, Amy T. Gilbert, James C. Beasley, Guha Dharmarajan, Olin E. Rhodea

United States Department of Agriculture Wildlife Services: Staff Publications

terrestrial wildlife species. However, there is currently a limited understanding of how different baiting strategies influence raccoon (Procyon lotor) uptake of ORV baits in non-agricultural habitats in the Southeastern United States, which may hinder the refinement of ORV management for raccoons in this region. Using a combination of new and existing data, we investigated the role of baited area (0.16 vs 3 km²), bait density (75 versus 150 baits/km²) and season (spring versus fall) on placebo ORV bait uptake by raccoons in four rural, non-agricultural habitats in the southeastern United States (bottomland hardwood forest, …