Population Biology Commons^™

A New Reproductive Mode In The Genus Melanzophryniscus Gallardo, 1961 (Anura: Bufonidae) With Description Of A New Species From The State Of Paraná, Brazil, José A. Langone, Magno V. Segallo, Marcos Bornschein, Rafael O. De Sá

Biology Faculty Publications

A new species of bufonid toad of the genus Melanophryniscus is described from a mountaintop that is part of the Serra do Mar in the northeastern State of Paraná, Brazil. Melanophryniscus alipioi sp. nov. is distinguished from other known species by its uniformly dark brown dorsal color and a unique breeding site. The new species reproduces in bromeliads, a reproductive mode previously unknown for this genus. This species might be susceptible to current habitat lost.

The Horseshoe Crab Conundrum: Can We Harvest And Conserve?, Jennifer Mattei, Mark Beekey

Biology Faculty Publications

Horeshoe crabs "Limulus polyphemus" are remarkable ‘living fossils’ which have unique blood cells (amebocytes) that are used to test human vaccines for bacterial contamination. In the 1950’s, scientists at the Marine Biological Laboratory in Woods Hole, Massachusetts, not only discovered amebocytes but also found that they had special properties. If the amebocytes came into contact with bacteria, they would instantly coagulate around the bacteria and attack it. The Woods Hole scientists took this unique property of horseshoe crabs and developed a test for bacterial contamination using a horseshoe crab blood derivative called Limulus Amebocyte Lysate (LAL).

This article describes discusses …

Project Limulus: What Long-Term Mark/Recapture Studies Reveal About Horseshow Crab Population Dynamics In Long Island Sound, Mark Beekey, Jennifer Mattei

Biology Faculty Publications

Project Limulus is a long-term study of the population dynamics of the horseshoe crab population in Long Island Sound (LIS). We have tagged over 20,000 spawning adults from >20 beaches ranging from Greenwich to Stonington, CT since 1997. Cumulative recapture rates have reached 9%. On average 90% of the crabs are recaptured within a few miles of their original tag site within the first season. Between seasons, on average, 45% of crabs are recaptured within the same locality of where they were tagged. Of all recaptures, 99% of recaptured individuals are found within LIS. This past year we expanded the …

Designation Of A Neotype For Leptodactylus Gracilis (Duméril And Bibron, 1840) (Amphibia: Leptodactylidae), Rafael O. De Sá, A. Dubois, A. Ohler

Biology Faculty Publications

The examination of the preserved specimens in the herpetological collection of the Museum National d'Histoire Naturelle, along with a review of the original literature, indicates that no extant specimen can be undoubtedly identified as the holotype of Leptodactylus gracilis (DumCri.l and Bibron, 1840). Furthermore, it revealed that the type locality recently assigned to this taxon is in error.

Eleutherodactylus Ridens (Pygmy Rainfrog) Predation, Tobias Eisenberg, Twan Leenders

Biology Faculty Publications

Workers of the large ponerine ant Paraponera clavata typically forage on small to medium-sized arthropods or collect pieces of plants or nectar but have been suspected of predating small vertebrates.

Description Of The Tadpole Of Leptodactylus Pustulatus Peters, 1870 (Anura: Leptodactylidae), Rafael O. De Sá, Reuber Brandão, Lorena Dall'ara Guimarães

Biology Faculty Publications

The external morphology and oral disc of the tadpole of Leptodactylus pustulatus Peters, 1870, are described and illustrated for Gosner stage 39. The internal oral anatomy was analyzed with SEM at Gosner stage 36 whereas chondrocranial anatomy is reported for Gosner stage 35. The overall characteristics do not depart from those known for the genus Leptodactylus and they particularly agree for those of the melanonotus species group. The labial tooth row formula is 2(2)/3. Observations are presented that suggest that L. pustulatus engages in larval parental care.

Resumo

São descritas a morfologia externa e o disco oral do girino de …

The Tadpole Of Eupsophus Queulensis (Anura, Cycloramphidae), Diana R. Cárdenas-Rojas, Alberto Veloso, Rafael O. De Sá

Biology Faculty Publications

The genus Eupsophus is characterized by endotrophic larvae that inhabit water-filled cavities. The larvae feed from large yolk reserves during metamorphosis. The external morphology has been described for four of the ten known species and data on larval chondrocranial morphology are available only for one. We describe the extema1 morphology, oral disc and chondrocranial anatomy of the larvae of Eupsophus queulensis. The characteristics of E. queulensis tadpoles are compared with those of other Eupsophus larvae.

The Tadpole Of Leptodactylus Notoaktites Heyer, 1978 (Anura, Leptodactylidae), Rafael O. De Sá, José A. Langone, Magno V. Segalla

Biology Faculty Publications

The external morphology and oral disc of the tadpole of Leptodactylus notoaktites Heyer, 1978, are described and illustrated for Gosner’s stage 33. The internal oral anatomy was analyzed under SEM at Gosner’s stage 36 whereas chondrocranial anatomy is reported for Gosner’ stage 38. The morphology of this tadpole is compared with those available for other species of the L. mystaceus complex. The overall characteristics do not depart from those known for the genus Leptodactylus and they particularly agree for those of the fuscus species group. The labial tooth row formula is 2(2)/3.

Karyotypes Of Eight Species Of Leptodactylus (Anura, Leptodactylidae) With A Description Of A New Karyotype For The Genus, Renata Cecília Amaro-Ghilardi, Gabriel Skuk, Rafael O. De Sá, Miguel Trefaut Rodrigues, Yatiyo Yonenaga-Yassuda

Biology Faculty Publications

Karyotypes of eight species of Leptodactylus (Anura, Leptodactylidae) with a description of a new karyotype for the genus. Eight species of the Neotropical genus Leptodactylus were karyologically studied: seven of them (L. gracilis, L. mystacinus, L. petersii, L. pustulatus, L. macrosternum, L. ocellatus, L. labyrinthicus) presented 2n=22 and L. silvanimbus showed a distinctive karyotype with 2n=24. Nucleolar organizer regions (Ag-NORs) were detected in two different pairs of chromosomes: pair 4 at the proximal region of the long arm of one individual of L. mystacinus from São Paulo state and of L. petersii; and …

Genetic Resolution Of The Enigmatic Lesser Antillean Distribution Of The Frog Leptodactylus Validus (Anura, Leptodactylidae), Keneth Yanek, W. R. Heyer, Rafael O. De Sá

Biology Faculty Publications

Leptodactylus validus has an unusual distribution, inhabiting Trinidad, Tobago, and the Lesser Antilles, but not the mainland of South America. This distribution is inconsistent with other distribution patterns observed for these islands. Although slight variation in adult morphology has been observed among the different island populations of L. validus, call data suggest the presence of a single species. Calls of L. pallidirostris from Venezuela and Brazil suggested that this taxon might be conspecific with L. validus. Sequence data from the 12S and 16S mt rDNA genes indicate that L. validus represents a single species throughout its distribution and is conspecific …

New Distribution Records For Amphibians And Reptiles In Connecticut, With Notes On The Status Of An Introduced Species, Gregory J. Watkins-Colwell, Twan Leenders, Brian T. Roach, Daniel J. Drew, Gregg Dancho, Jeanne Yuckienuz

Biology Faculty Publications

Recent field work and a review of catalogued specimens in the herpetology collections at the Yale Peabody has yielded 170 new town records for amphibians and reptiles in Connecticut. These are reported here, along with observations on the status of the red-ear slider (Trachemys scripta) in Connecticut.

Rana Ocellata Linnaeus, 1758 (Currently Leptodactylus Ocellatus; Amphibia, Anura): Proposed Conservation Of Usage Of The Specific Name By The Designation Of A Neotype, W. Ronald Heyer, Ulisses Caramaschi, Rafael O. De Sá

Biology Faculty Publications

The purpose of this application, under Article 75.6 of the Code, is to conserve the usage of the specific name of Rana ocellata Linnaeus, 1758 for a species of leptodactylid frog from South America by the designation of a neotype. Prevailing usage of the name is threatened by the identity of the type specimen which is a different species than that which is currently known as Leptodactylus ocellatus. It is proposed that all name-bearing types be set aside and a neotype designated in accord with prevailing usage.

Negative Effect Of Zebra Mussels On Foraging And Habitat Use By Lake Sturgeon (Acipenser Fulvescens), D. J. Mccabe, Mark Beekey, A. Mazloff, I. E. Marsden

Biology Faculty Publications

1. Lake sturgeon (Acipenser fulvescens) are threatened or endangered throughout much of their range. Juvenile sturgeon utilize sandy and silty habitats extensively during their growth. Invasive zebra mussels change the nature of sandy and silty habitats because they settle on and coat the habitat with the shells of living and dead individuals. The potential impacts of this increased habitat complexity on lake sturgeon is unknown. 2. Juvenile lake sturgeon habitat choice was assessed in laboratory experiments, and zebra mussel impact on the foraging success of juvenile lake sturgeon on three different prey species was measured. 3. Sturgeon foraging on chironomids …

The Distress Calls Of Leptodactylus Chaquensis Cei, 1950 And Leptodactylus Elenae Heyer, 1978 (Anura: Leptodactylidae), José M. Padial, Rafael O. De Sá, Ignacio De La Riva

Biology Faculty Publications

We describe the distress calls of Leptodactylus chaquensis and Leptodactylus elenae. They each consist of a single, long, tonal sound with complex harmonic structure and complex frequency modulation. The distress calls of both species are different from their respective advertisement calls. Moreover, there are also clear interspecific differences. Harmonic frequencies in L. elenae are higher, there are fewer emphasized harmonics and the call is shorter and repeated at a higher rate (sometimes paired) than the call of L. chaquensis. Call duration, call rate, and position of the dominant harmonic change with the strength of the stimulus that the …

Leptodactylus Fragilis (Brocchi): White-Lipped Thin-Toed Frog, Miriam M. Heyer, W. Ronald Heyer, Rafael O. De Sá

Biology Faculty Publications

Adult Leptodactylus fragilis are small. The head is longer than wide, and the hind limbs are moderately long (Table 1; Heyer and Thompson 2000 provided definitions of adult size and leg length categories for Leptodactylus). Male vocal sacs are laterally expanded and range from lightly gray speckled to darkly pigmented. Male snout is spatulate; female snout is rounded. Male forearms are not hypertrophied and males lack asperities on the thumbs and chest. The dorsum is spotted or blotched with dark markings, which are often chevron-shaped and sometimes confluent. The supratympanic fold is dark brown. A pair of dorsolateral folds …

A Phylogenetic Analysis Of Vanzolinius Heyer, 1974 (Amphibia, Anura, Leptodactylidae): Taxonomic And Life History Implications, Rafael O. De Sá, W. Ronald Heyer, Arley Camargo

Biology Faculty Publications

The validity of the monotypic leptodactylid frog genus Vanzolinius Heyer, 1974 has been questioned recently. We explore the relationship of Vanzolinius discodactylus within the cluster of closely related genera Adenomera, Leptodacylus, and Lithodytes with both morphological and molecular data sets. Morphological and combined morphological and molecular data were analyzed using maximum parsimony; molecular data sets were analyzed with maximum likelihood methods. The resultant relationships are unambiguous in Vanzolinius being imbedded within Leptodactylus. In order to maintain Leptodactylus as a monophyletic genus, Vanzolinius is placed in the synonymy of Leptodactylus Fitzinger, 1826. The implications of relationships analyzed in …

Redescripción De La Morfología Larval Externa De Dos Especies Del Grupo De Leptodactylus Fuscus (Anura, Leptodactylidae), José A. Langone, Rafael O. De Sá

Biology Faculty Publications

Re-description of the larval external morphology of two species of the Leptodactylus fuscus group (Anura, Leptodactylidae). The larvae of Leptodactylus gracilis (D’Orbigny y Bibron, 1840) and L. mystacinus Bumeister, 1861 are re-described and compared with previous descriptions noting intraspecific variation in oral disc characteristics (arrangement of papillae and rows of cornified teeth). The external morphological analysis of larvae of the Leptodactylus fuscus group suggest that the lack of comparable descriptions among species, as well as the lack of analyses of their intraspecific variation, limits the use of larval characteristics for diagnostic purposes.

"Sibling Species, Advertisement Calls, And Reproductive Isolation In Frogs Of The Leptodactylus Pentadactylus Species Cluster (Amphibia, Leptodactylidae), W. Ronald Heyer, Rafael O. De Sá, A. Rettig

Biology Faculty Publications

A recent re-evaluation of morphological and advertisement call variation in the large species of frogs of the Leptodactylus pentadactylus cluster discovered more examples of sibling species as defined by Ernst Mayr in his influential book Animal Species and Evolution. All previously documented instances of sibling species in frogs demonstrated advertisement call differentiation consistent with the calls serving as pre-mating isolating mechanisms. However, we find one instance of two species with nondistinguishable adult morphologies as well as nondistinguishable advertisement calls. Presumably, the new instances of sibling species reflect retention of ancestral adult morphologies and advertisement calls. Larval and habitat differentiation appear …

Are Leptodactylus Didymus And L. Mystaceus Phylogenetically Sibling Species (Amphibia, Anura, Leptodactylidae)?, Rafael O. De Sá, W. R. Heyer, Arley Camargo

Biology Faculty Publications

The Leptodactylus fuscus species group consists of 25 currently recognized species; within this species group and distributed throughout the Amazon Basin, Atlantic Forests, Gran Chaco, and cerrados is the L. mystaceus species complex. This species complex consists of L. didymus, L. elenae, L. mystaceus, L. notoaktites, and L. spixi. Adult morphologies have been used to distinguish these species from each other except for L. didymus and L. mystaceus (Heyer, 1978; Heyer et al., 1996). Leptodactylus didymus and L. mystaceus are morphologically indistinguishable; the species are recognizable only by the characteristics of their advertisement calls: non-pulsed in L. didymus and pulsed …

Common Names For The Frog Genus Leptodactylus (Amphibia, Anura, Leptodactylidae), Ulisses Caramaschi, Rafael O. De Sá, W. Ronald Heyer

Biology Faculty Publications

Most species of the frog genus Leptodactylus lack common names. Common names are those names used by different human cultures and societies for the species of animals and plants where humans and biota reside together. Often times common names are lacking for Neotropical frogs or are broad in scope. For example, indigenous people may have a single name for frogs that occur on the ground and another name for frogs that occur in shrubs and trees, even though there are many species of ground frogs called by the same name as well as for the tree frogs. Sometimes colonists have …

Crisis Global De Biodiversidad: Importancia De La Diversidad Genética Y La Extinción De Anfibios, Rafael O. De Sá

Biology Faculty Publications

El mundo enfrenta, por primera vez desde el comienzo de la civilización, el declive y la extinción de una clase de vertebrados, los anfibios. Esta extinción aparenta ser un evento global y de la magnitud que llevó a la desaparición de los dinosaurios. Muchas son las posibles causas de esta extinción y el efecto combinado de estos factores agravan la situación. La pérdida de ambientes naturales permanece como el factor más crítico en el declive de anfibios, pero enfermedades infecciosas emergentes parecen ser responsables de mortalidades masivas en décadas recientes. El declive y extinción de anfibios representa una llamada urgente …

Apparent Sibling Rivalry In The Freshwater Clam Sphaerium Striatinum, Mark Beekey, Ronald H. Karlson

Biology Faculty Publications

In Sphaerium striatinum, a freshwater brooding bivalve, up to 97.5% of offspring that adults initially produce fail to reach independence. Marsupial sacs, specialized extensions of gill filaments that act as nurseries, initially contain multiple offspring in various sizes and stages of development. However, by the time offspring reach later stages of development, marsupial sacs typically contain only one offspring. Brood mortality is hypothesized to be the result of competition among embryos for nutrients and/or space. Sphaeriid eggs do not contain enough yolk for offspring to complete development. Adults supply additional nutrients required to reach independence. Brood capacity is limited by …

Zebra Mussels Affect Benthic Predator Foraging Success, Mark Beekey, D. J. Mccabe, I. E. Marsden

Biology Faculty Publications

The introduction of zebra mussels (Dreissena spp.) to North America has resulted in dramatic changes to the complexity of benthic habitats. Changes in habitat complexity may have profound effects on predator-prey interactions in aquatic communities. Increased habitat complexity may affect prey and predator dynamics by reducing encounter rates and foraging success. Zebra mussels form thick contiguous colonies on both hard and soft substrates. While the colonization of substrata by zebra mussels has generally resulted in an increase in both the abundance and diversity of benthic invertebrate communities, it is not well known how these changes affect the foraging efficiencies of …

A New Species Of Callulina (Anura: Microhylidae) From The West Usambara Mountains, Tanzania, Rafael O. De Sá, Simon P. Loader, Alan Channing

Biology Faculty Publications

The description of the species Callulina kreffti was based on specimens collected in the East Usambara Mountains of Tanzania. Successive collecting has shown this species to be widely distributed through the Eastern Arc Mountains. Advertisement calls from populations in the type locality of Callulina kreffti were compared with calls from populations in the West Usambara Mountains. Analysis of the calls suggested that these two populations of Callulina represent two separate taxa. Subsequent morphological and molecular investigations indicated that these two populations are distinct. Herein, we describe a new Callulina species on the basis of call, morphology and molecular sequences.

A New Toad (Anura: Bufonidae) From Uruguay, Raúl Maneyro, Diego Arrieta, Rafael O. De Sá

Biology Faculty Publications

A new species of toad is described from small streams in the hills of Cuchilla de Haedo and Cuchilla Grande in northeastern Uruguay. Specimens of the new species were previously mistaken for Bufo arenarum; they differ from B.arenarum by having elongated parotoid glands and reddish-brown parotoid and cephalic crests. A discriminant analysis resulted in three groups corresponding to B. arenarum, Bufo rufus, and the specimens herein described as a new species.

Distribución Geográfica De La Fauna De Anfibios Del Uruguay, Diego Nuñez, Raúl Maneyro, José A. Langone, Rafael O. De Sá

Biology Faculty Publications

Datos precises sobre la distribución geográfica de los organismos de una región son necesarios y prerrequisito para entender las interrelaciones ecológicas entre las comunidades biológicas. Esta información también contribuye a entender el componente histórico de la región posibilitando la determinación de centros de origen y el establecimiento de patrones de diversidad (Ron, 2000), así mismo posibilita realizar inferencias acerca de los mecanismos de especiación en los diferentes grupos zoológicos (Bridarolli & Di Tada, 1994).

La distribución geográfica de los anfibios en Uruguay ha sido parcialmente reportada (Achaval & Olmos, 1997; Langone, 1995; Maneyro et al, 1995; Maneyro & Langone, 2001). …

Características Histológicas De La Tumefacción Frontal De Melanophryniscus (Amphibia: Anura: Bufonidae), Daniel E. Naya, José A. Langone, Rafael O. De Sá

Biology Faculty Publications

Se analizó la histología tegumentaria de la región cefálica en seis especies del género Melanophryniscus, pertenecientes a los grupos stelzneri (4) y tumifrons (2), con el objetivo de esclarecer la estructura de la tumefacción frontal y su posible función. Las características generales de la histología del grupo stelzneri, coincidieron con las descripciones tegumentarias existentes para el género. Sin embargo, la región cefálica frontal en las dos especies del grupo tumifrons muestran cambios histológicos correlacionados a la tumefacción frontal. Esta estructura presenta una organización similar a otras macroglándulas presentes en la dermis de anuros (e.g., parotoides, paracnémicas), y por tanto se …

On The Engimatic Distribution Of The Honduran Endemic Leptodactylus Silvanimbus (Amphibia: Anura: Leptodactylidae), W. R. Heyer, Rafael O. De Sá, Sarah Muller

Biology Faculty Publications

Most species of the frog genus Leptodactylus occur in South America, and all authors who have treated the zoogeography of the genus have concluded that it originated somewhere in South America (e.g., Savage 1982). Savage (1982, 518) summarized the historical herpetofaunal units of the Neotropics as follows: "All evidence points to an ancient contiguity and essential similarity of a generalized tropical herpetofauna that ranged over tropical North, Middle, and most of South America in Cretaceous-Paleocene times. Descendents of this fauna are represented today by the South and Middle American tracks (Elements). To the north of this fauna ranged a subtropical-temperate …

Effect Of Food Availability On Reproduction And Brood Size In A Freshwater Brooding Bivalve, Mark Beekey, Ronald H. Karlson

Biology Faculty Publications

Brood size is often constrained by the amount of energy available to produce offspring. Yet, energetic constraints may not be as important if the physical capacity to brood offspring is limited. Investigating the relative importance of energetic and physical constraints on brood size is necessary to understand how reproductive strategies are molded by natural selection. We investigated how food availability affects brood size in Sphaerium striatinum, a freshwater bivalve. We reared juveniles to adulthood under three food levels in a common garden experiment. The number of reproductive attempts, brood size, and stage of offspring development were measured. Clams reared …

The Tadpole Of Phrynobatrachus Mababiensis Fitzsimons, 1932 (Anura, Ranidae, Petropedetinae), Rafael O. De Sá, Alan Channing

Biology Faculty Publications

The puddle frog genus Phrynobatrachus Günther, 1862 comprises about 64 currently recognized species (Frost, 1985). Of these, only the tadpoles of P. natalensis (Smith, 1849) (Power, 1927; Channing, 2001), P. guineensis Guibé & Lamotte, 1961(Rödel,1998) and P. alticola Guibé & Lamotte, 1961 (Rödel & Ernst, 2002) have been described. Phrynobatrachus mababiensis FitzSimons, 1932 (Dwarf Puddle frog, Wager, 1986; Mababe River frog, Frank & Ramus, 1996) is a small frog that usually calls from low in thick vegetation on flooded terrains close to the water. Very little has been published about the biology of this species. Passmore & Carruthers (1979) reported …