Open Access. Powered by Scholars. Published by Universities.®
Other Cell and Developmental Biology Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Discipline
- Institution
- Keyword
Articles 1 - 3 of 3
Full-Text Articles in Other Cell and Developmental Biology
Epigenetic Modifications In Dedifferentiated Liver Cells To Restore Lost Liver Function, Shreya Sharma
Epigenetic Modifications In Dedifferentiated Liver Cells To Restore Lost Liver Function, Shreya Sharma
Masters Theses
The goal of the study is to investigate whether the epigenetic modifications can enhance the restoration of liver gene expression in dedifferentiated hepatoma cells or not. For the study, we used dedifferentiated H11 hepatoma cell lines which overexpress one of a panel of introduced candidate liver-enriched transcription factors H11-HNF1a, H11-CREB3L3, H11-CREG1, H11- HHEX, H11-HNF6, and H11-IGFBP1. The cells were treated with the DNA methyltransferase inhibitor 5-azacytidine and the histone deacetylase inhibitor sodium butyrate, both individually and in combination. Gene expression analysis was determined by RT-qPCR, which revealed that while transcription factor overexpression alone produced limited activation, epigenetic modulation, particularly Histone …
Neurogenesis And Functional Recovery Of Murine Retinal Ganglion Cells After Glaucoma Induction Following Treatment With Pnu-282987, Samantha Luzadre
Neurogenesis And Functional Recovery Of Murine Retinal Ganglion Cells After Glaucoma Induction Following Treatment With Pnu-282987, Samantha Luzadre
Masters Theses
Experiments were performed to demonstrate electrical recovery in glaucoma-induced adult mice after neurogenesis of retinal ganglion cells (RGCs). Newly derived RGCs were induced following eye drop treatment with a selective alpha7 nicotinic acetylcholine receptor agonist, PNU-282987. Both sexes of transgenic (RlbpCre-ER (CRE+) and Rosa-tdTomato (tdTomato+)) adult mice were utilized to trace newly generated RGCs originating from Müller glia (MG). Experimental mice were injected with hypertonic saline into the episcleral vein of anesthetized mice to significantly increase intraocular pressure (IOP) and induce glaucoma-like conditions. One month after the injection and after significant death of RGCs occurred, mice …
The Effects Of Deleting Lrb E3 Ligase Region 2 On Red Light Responses In Arabidopsis Thaliana, Sabina Liskey
The Effects Of Deleting Lrb E3 Ligase Region 2 On Red Light Responses In Arabidopsis Thaliana, Sabina Liskey
Masters Theses
The ability of plants to absorb light through photoreceptors is essential for successful plant development. Phytochromes are important plant photoreceptors that detect and absorb red and far-red light and must function properly for plant survival. The Light Response BTB proteins (LRB) play an important role in a plant’s response to red light by degrading phytochrome B (PhyB) via the ubiquitin proteasome system (UPS). Although there has been a successful investigation into understanding the function of the LRBs, the function of a few regions within LRB proteins remains unknown, including a portion located on the N-terminal end of the protein, termed …