Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Biology (5)
- Biochemistry, Biophysics, and Structural Biology (4)
- Cell Biology (4)
- Molecular Biology (4)
- Genetics and Genomics (3)
-
- Computational Biology (2)
- Genetics (2)
- Neuroscience and Neurobiology (2)
- Bioinformatics (1)
- Cell Anatomy (1)
- Developmental Neuroscience (1)
- Ecology and Evolutionary Biology (1)
- Genomics (1)
- Integrative Biology (1)
- Medical Neurobiology (1)
- Medical Sciences (1)
- Medicine and Health Sciences (1)
- Molecular and Cellular Neuroscience (1)
- Neurosciences (1)
- Structural Biology (1)
- Institution
- Publication
- Publication Type
Articles 1 - 13 of 13
Full-Text Articles in Developmental Biology
Tmem116 Crispr-Mediated Knockout Prevents Proper Lens Regeneration In Iberian-Ribbed Newts, Damien T. Mann
Tmem116 Crispr-Mediated Knockout Prevents Proper Lens Regeneration In Iberian-Ribbed Newts, Damien T. Mann
Honors Theses and Capstones
Eye diseases like cataracts, age-related macular degeneration (AMD), and glaucoma are widespread and detrimental conditions affecting many people across the globe. While there are reliable and successful treatments for those with cataracts that can reverse vision impediments, others like glaucoma and AMD have limited treatment options. Additionally, eye damage resulting from these conditions is commonly irreversible, establishing a need for new treatment options. The Iberian-ribbed newt, Pleurodeles waltl, is a salamander species primarily studied for their amazing regenerative capabilities. Particularly, these newts are capable of de novo lens regeneration after a complete resection, providing a unique opportunity to study regeneration …
Regulation Of Embryonic Eye Regrowth In Xenopus Laevis, Dylan Guerin
Regulation Of Embryonic Eye Regrowth In Xenopus Laevis, Dylan Guerin
UNLV Theses, Dissertations, Professional Papers, and Capstones
Regrowth of lost organs and tissues is an amazing ability that some animals possess. For regrowth to be considered full, the organism must regrow the organ or tissue to a state that is structurally and functionally similar to that which was lost. The ability of an organism to regrow lost or damaged tissue varies among animals, both in the mechanisms utilized to achieve regrowth, and in what body parts can be regrown. Potentially even closely related species can vary wildly in regenerative ability. Therefore, regenerative research can look to other tissues and organisms for direction in elucidating the mechanisms through …
Investigating Conserved Mechanisms For Embryonic Eye Regrowth In Xenopus Laevis, Cindy Kha
Investigating Conserved Mechanisms For Embryonic Eye Regrowth In Xenopus Laevis, Cindy Kha
UNLV Theses, Dissertations, Professional Papers, and Capstones
Regeneration is a complex process to replace or restore tissue after damage. However, animals vary in their ability to regenerate tissue, and humans are limited in their abilities. Understanding how regeneration occurs in highly regenerative vertebrates, such as in Xenopus laevis frogs will lead to defining common regeneration mechanisms. In recent years, eye regeneration research in Xenopus laevis tadpoles provided new promising insight into the mechanisms necessary for repair of damaged eye tissue. Much progress has been made towards understanding the regenerative capability of the complex eye since Xenopus tadpoles can regenerate mature eye tissues, including: the optic nerve, retina, …
The Effects Of Aging On Wound Healing And Limb Regeneration In The Axolotl, Hande Sahin
The Effects Of Aging On Wound Healing And Limb Regeneration In The Axolotl, Hande Sahin
Graduate Masters Theses
The Mexican axolotl is capable of regenerating various parts of its body and maintains this capacity throughout its entire life. Although this ability is sustained through adulthood, multiple regenerative processes appear to be negatively affected by aging. Here, we focus on evaluating the effect of aging on the rate of wound healing and limb regeneration. We have developed new in vivo, and ex vivo assays to characterize wound healing and identify differences between young and aged animals during this process. We have also characterized morphological features of mature skin from both groups of animals and although there are no obvious …
Investigating The Potential Role For The Nervous System In Controlling Regeneration In Nematostella Vectensis, Kristen Malir
Investigating The Potential Role For The Nervous System In Controlling Regeneration In Nematostella Vectensis, Kristen Malir
Biological Sciences Undergraduate Honors Theses
Nematostella vectensis is a marine sea animal that has become a model for developmental and evolutionary research. Included in the phylum Cnidaria, N. vectensis’ was chosen as the model for this research. Not only can this animal go through asexual and sexual reproduction, but it also has the ability to regenerate. Although much research has been put forth in an effort to understand regeneration better, much is still unknown. The underlying mechanisms of regeneration in Cnidaria are illusive; however, studies within vertebrates have shown the substantial role of the nervous system. The objective of this experiment is to test if …
Identifying Epidermal Enriched Genes Required For Planarian Regeneration- Sp. Schmidtea Mediterranea, Pallob Barai
Identifying Epidermal Enriched Genes Required For Planarian Regeneration- Sp. Schmidtea Mediterranea, Pallob Barai
Theses and Dissertations--Biology
The outer epithelial layer covering an organism, commonly known as the epidermis, is crucial for maintaining homeostasis and for the wound healing processes after injury. The planarian epidermis allows flatworms to heal their wounds and virtually restore any missing tissues. Immediately after amputation, planarians contract their muscle and stretch their epidermis to heal the wound area. However, how the planarian epidermis coordinates with other tissues and mechanisms after the initial wound healing processes begins is not understood in detail. I hypothesized that epidermal cell stretching upon wound healing induces transcriptional changes that are required for effective regeneration. To test this …
Leveraging Transcriptomic Approaches To Identify Differences In Genetic Programming Driving Two Distinct Wound Healing Mechanisms, Regeneration And Fibrosis, In Acomys And Mus, Shishir K. Biswas
Theses and Dissertations--Biology
Why can some animals and others cannot? This fundamental question has fueled scientists studying regeneration for hundreds of years since early observations in crayfish, salamanders and many other organisms. While most contemporary work in regeneration is done in a handful of species including salamanders, zebrafish and flatforms, these organisms lack a closely-related, non-regenerating sister species from which unique genetic differences can be identified. Additionally, while much has been learned from these organisms, they do not share fundamental biological traits with mammals (endothermy, metabolism and immune system) which limits the ability to translate this research for clinical medicine. To this end, …
Connective Tissue Fibroblasts From Highly Regenerative Mammals Are Refractory To Ros-Induced Cellular Senescence, Sandeep Saxena, Hemendra J. Vekaria, Patrick G. Sullivan, Ashley W. Seifert
Connective Tissue Fibroblasts From Highly Regenerative Mammals Are Refractory To Ros-Induced Cellular Senescence, Sandeep Saxena, Hemendra J. Vekaria, Patrick G. Sullivan, Ashley W. Seifert
Biology Faculty Publications
A surveillance system in mammals constantly monitors cell activity to protect against aberrant proliferation in response to damage, injury and oncogenic stress. Here we isolate and culture connective tissue fibroblasts from highly regenerative mammals (Acomys and Oryctolagus) to determine how these cells interpret signals that normally induce cellular senescence in non-regenerating mammals (Mus and Rattus). While H2O2 exposure substantially decreases cell proliferation and increases p53, p21, p16, and p19 in cells from mice and rats, cells from spiny mice and rabbits are highly resistant to H2O2. Quantifying oxygen consumption …
Retinoic Acid Receptor Isoform-Specific Control Of Mouse Salivary Gland Development And Regeneration, Kara Desantis
Retinoic Acid Receptor Isoform-Specific Control Of Mouse Salivary Gland Development And Regeneration, Kara Desantis
Legacy Theses & Dissertations (2009 - 2024)
Controlled expansion and differentiation of progenitor cell populations is essential for organogenesis followed by continued maintenance of the population into and through adulthood. As the K5+ basal cell population is regulated by retinoic acid signaling, we interrogated the contribution of specific RAR isoforms to the regulation of these cells during submandibular salivary gland (SMG) organogenesis and regeneration. Retinoic acid has previously been shown to be involved in the development of the salivary gland, and recently, lack of retinoid signaling has been shown to impact the K5+ population of basal progenitor cells. Since retinoic acid is known to exert stimulatory effects …
Pcdh19 Expression In Normal And Regenerating Adult Zebrafish Retinas, Kristin N. Forkapa Ms.
Pcdh19 Expression In Normal And Regenerating Adult Zebrafish Retinas, Kristin N. Forkapa Ms.
Williams Honors College, Honors Research Projects
My Honors Research Project will test the function of the N-cadherin molecule on the zebrafish optic nerve regeneration. N-cadherin is a member of the cadherin superfamily. Cadherins are cell adhesion molecules important for animal development and maintenance of adult structures. Unlike mammals, fish and amphibians have the ability to regenerate their optic nerve after damages. Molecular mechanisms underlying the optic nerve regeneration are still under intense investigation. Studies in Dr. Liu’s laboratory showed that expression of several cadherins, including N-cadherin, was greatly increased during adult zebrafish optic nerve regeneration, suggesting that cadherins may be involved in the optic nerve regeneration …
Beryllium Nitrate Supports Fibroblast Migration As An Essential Component Of Skin And Limb Regeneration In Axolotls, Adam Boyd Cook
Beryllium Nitrate Supports Fibroblast Migration As An Essential Component Of Skin And Limb Regeneration In Axolotls, Adam Boyd Cook
Theses and Dissertations--Biology
Tissue regeneration in salamanders is a robust process that is not easily interrupted or altered. Therefore, inhibiting regeneration provides a means to interrogate the underlying cellular and molecular mechanisms regulating this complex event. Here we show that application of a relatively low concentration of beryllium nitrate solution (100mM) causes a delay in skin regeneration and severely alters normal limb regeneration. We provide evidence showing a beryllium-induced reduction in dermal fibroblast migration in vivo and in vitro. We link this phenomenon to delayed regeneration of the skin and abnormal blastema formation resulting in limb patterning defects during regeneration. Though our …
Characterization Of Adult Zebrafish Retinal Regeneration Following Two Different Damage Models, Jennifer Lee Thomas
Characterization Of Adult Zebrafish Retinal Regeneration Following Two Different Damage Models, Jennifer Lee Thomas
Wayne State University Dissertations
Unlike mammals, zebrafish can regenerate all of their retinal neurons through Müller glial cells, which respond to retinal damage by re-entering the cell cycle to create clusters of progenitor cells. The progenitors continue to proliferate as they migrate to the site of damage, where they ultimately differentiate into new retinal neurons. In contrast, Müller glia of the mammalian retina respond to injury with reactive gliosis, which if persistent, can lead to loss of Müller cell function and devastating vision loss. Despite this, multiple lines of evidence suggest that mammalian Müller glial cells possess a latent ability to regenerate retinal neurons. …
Extracellular Macromolecules In Regenerating Sabellids: A Histochemical Study, Michael R. Bigelow
Extracellular Macromolecules In Regenerating Sabellids: A Histochemical Study, Michael R. Bigelow
MUSC Theses and Dissertations
Regenerating sabellid polychaetes were histochemically studied with regard to the synthesis and deposition of glycosaminoglycans (GAG) and collagen. Both of these types of macromolecules are known to be actively involved in cell migrations and differentiations of several vertebrate systems. The purpose of this study was to investigate whether or not these extracellular materials might be similarly involved in invertebrate systems. Results demonstrate for the first time that these same extracellular materials, GAG and collagen, actively participate during invertebrate development. Production of hyaluronic acid is shown to accompany mesenchymal migration and blastema formation. Deposition of sulfated GAG collagen in the form …