Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Cell Biology (6)
- Biology (4)
- Biochemistry (3)
- Biochemistry, Biophysics, and Structural Biology (3)
- Molecular Biology (3)
-
- Animal Sciences (2)
- Ecology and Evolutionary Biology (2)
- Genetics and Genomics (2)
- Genomics (2)
- Integrative Biology (2)
- Marine Biology (2)
- Other Physiology (2)
- Physiology (2)
- Biodiversity (1)
- Biotechnology (1)
- Cellular and Molecular Physiology (1)
- Comparative and Evolutionary Physiology (1)
- Environmental Health (1)
- Evolution (1)
- Genetics (1)
- Microbiology (1)
- Molecular and Cellular Neuroscience (1)
- Neuroscience and Neurobiology (1)
- Other Animal Sciences (1)
- Other Biochemistry, Biophysics, and Structural Biology (1)
- Other Cell and Developmental Biology (1)
- Other Ecology and Evolutionary Biology (1)
- Keyword
-
- Drosophila (2)
- Embryo (2)
- AMH (1)
- Anoxia (1)
- Artemia franciscana (1)
-
- Caenorhabditis elegans microbiome (1)
- Cellular aging (1)
- Complex I (1)
- Crude Oil Photo-products (1)
- DNA methylation (1)
- Desiccation Tolerance (1)
- Diapause (1)
- Endometrium (1)
- Epigenetics (1)
- Equine (1)
- Facultative vivipary (1)
- Gulf Killifish Embryos (1)
- Heat Stress (1)
- Immunohistochemistry (1)
- Insulin/IGF-1 signaling pathway (1)
- Intermediate Hydration (1)
- Intrinsically Disordered Protein (1)
- Late Embryogenesis Abundant Proteins (1)
- Life-history trade-off (1)
- Microbe–host interaction (1)
- Mitochondrial tabulation (1)
- Neuroblast (1)
- Nopp140 (1)
- Nucleolus (1)
- Oil Toxicity and Environmental Assessment (1)
- Publication Type
Articles 1 - 10 of 10
Full-Text Articles in Developmental Biology
Decoupling Of Reproduction And Lifespan Trade-Off In Caenorhabditis Elegans Through Microbial Programming, Nora M. Villafuerte
Decoupling Of Reproduction And Lifespan Trade-Off In Caenorhabditis Elegans Through Microbial Programming, Nora M. Villafuerte
LSU Master's Theses
The microbiome is increasingly recognized as a major regulator of host physiology. Using the Caenorhabditis elegans model, we investigated how natural commensal bacteria modulate host reproduction and lifespan through internal egg hatching, a phenotype in which retained embryos hatch within the maternal body. Screening strains from the CeMbio collection identified four focal strains Ochrobactrum BH3, Lelliottia JUb66, Pantoea BIGb0393, and Enterobacter CEent1 that significantly increased internal egg hatching during the late reproductive period. This contrasts with pathogenic bacteria, which induce early-onset internal egg hatching. Across the focal strains, worms exhibited reduced total brood size but an expanded reproductive window relative …
Effects Of Photo-Oxidized Crude Oil On Gulf Killifish Early Life Stages, Charles Campbell
Effects Of Photo-Oxidized Crude Oil On Gulf Killifish Early Life Stages, Charles Campbell
LSU Master's Theses
Photo-oxidation of spilled crude oil generates polar compounds that can heighten toxicity to marine fish embryos. We investigated the effects of photo-oxidized crude oil on early life stages of Gulf killifish (Fundulus grandis) by exposing embryos under flow-through conditions to water-accommodated fractions of four oil treatments: fresh oil unexposed to either sunlight or weathering referred to as dark control (DC), a UV-light oxidized whole oil (LT), the polar fraction extracted from LT (Pol) and the remnants after extracting the Pol from LT (NP). Oil loadings of 0.38–3.00 g oil/kg water were tested. Total PAH concentrations and chemical profiles …
Metabolic And Physiological Regulation In C. Elegans: Insights Into Sperm Activation And Peroxisome-Mediated Longevity, Yash Flora
LSU Doctoral Dissertations
Organelle dynamics lie at the heart of cellular signaling and metabolic regulation, orchestrating physiological processes such as reproduction and aging across the lifespan. In this dissertation, I investigate how organelle-based signaling coordinates sperm activation and modulates organismal aging in the nematode Caenorhabditis elegans. I identify SPIN-4, a previously uncharacterized Spinster family transporter, as a key regulator of spermiogenesis. SPIN-4 is strongly expressed in developing sperm, localizes to the plasma membrane, and promotes sperm activation by facilitating the export of sphingosine-1-phosphate (S1P). Loss of spin-4 results in defective pseudopod formation, reduced motility, and compromised fertility, phenotypes that can be rescued …
Metabolic Transitions In Embryos Of Artemia Franciscana: Regulation And Consequences For Oxidative Stress, Daniel Anthony Arabie
Metabolic Transitions In Embryos Of Artemia Franciscana: Regulation And Consequences For Oxidative Stress, Daniel Anthony Arabie
LSU Doctoral Dissertations
Embryos of Artemia franciscana survive harsh conditions in diapause and anoxia-induced quiescence for years by undergoing deep metabolic transitions. I investigated the relationship between phosphorylation of pyruvate dehydrogenase (PDH) and carbon flux to the tricarboxylic acid (TCA) cycle during metabolic depression. The difference in catalytic activity of the PDH complex (PDC) in maximally phosphorylated and dephosphorylated samples was 16.5-fold. Western blot analysis confirmed the changes in phosphorylation of PDH. Analysis of PDH expression in diapause and post-diapause embryos via phospho-PDH showed no significant differences. Investigation of post-diapause embryos under anoxia showed that phosphorylation of PDH decreased and activated the enzyme. …
A Study Of Tissue-Specific Rho Signaling Activation During Tubular Organ Formation In Drosophila, Charlotte Jean-Louise Adams
A Study Of Tissue-Specific Rho Signaling Activation During Tubular Organ Formation In Drosophila, Charlotte Jean-Louise Adams
LSU Master's Theses
Several organs within the human body are tubular in structure. In the event of a malfunction in the development of these tubular structures, maladies like spina bifida, anencephaly, and several other defects can arise. To understand the mechanisms of tubular organ formation, we use the Drosophila embryonic salivary gland (SG) as a model. During embryogenesis, SG cells undergo a series of cell shape changes, like apical constriction, to accommodate the developing gland. Apical constriction is regulated in part by the signal of the secreted ligand Folded gastrulation (Fog) and its G protein-coupled receptor (GPCR) at the cell membrane, which leads …
Insights Into Desiccation Tolerance: Properties Of Late Embryogenesis Abundant Proteins From Embryos Of Artemia Franciscana, Blase Matthew Leblanc
Insights Into Desiccation Tolerance: Properties Of Late Embryogenesis Abundant Proteins From Embryos Of Artemia Franciscana, Blase Matthew Leblanc
LSU Doctoral Dissertations
LEA proteins are a family of intrinsically disordered proteins that are expressed in various life stages of anhydrobiotic organisms and have been strongly associated with survival during water stress. The brine shrimp Artemia franciscana is the only known anhydrobiotic animal that expresses LEA proteins from Groups 1, 3, and 6. Here, I report that AfrLEA6, a novel Group 6 LEA protein, is most highly expressed in embryos during diapause and decreases throughout pre-emergence development. Notably, there is an acute drop in expression upon termination of the diapause state and the titer of AfrLEA6 during diapause is 10-fold lower than values …
The Drosophila Neuroblasts: A Model System For Human Ribosomopathies, Sonu Shrestha Baral
The Drosophila Neuroblasts: A Model System For Human Ribosomopathies, Sonu Shrestha Baral
LSU Doctoral Dissertations
This dissertation describes the use of Drosophila neuroblasts (NBs) to model human ribosomopathies; the overall goal is to understand why specific stem cell and progenitor cell populations are the primary targets in nucleolar stress as seen in the ribosomopathies. Chapter 1 provides an overview of relevant literature. Chapter 2 describes nucleolar stress in Drosophila neuroblasts as a model for human ribosomopathies. For this, we induce nucleolar stress by using the UAS-GAL4 system to express RNAi that depletes Nopp140 transcripts, and we also employ homozygous, CRISPR-Cas9-generated Nopp140 gene disruptions with a systemic null phenotype (Nopp140-/-). Embryonic lethality was observed …
Effect Of Heat Stress On Bovine Follicular Development And Epigenetic Changes, Fabian Andres Diaz
Effect Of Heat Stress On Bovine Follicular Development And Epigenetic Changes, Fabian Andres Diaz
LSU Doctoral Dissertations
Climate is one of the most important limiting factors in animal production. Cattle under the effect of heat stress have reduced fertility. Negative effects during gamete maturation and embryo development have been observed at the morphological, biochemical, transcriptional and developmental levels. Epigenetic mechanisms play a fundamental role in the regulation of gamete and embryo development. Several genes activated during gamete maturation and early embryo development are controlled by epigenetic mechanisms. There are no studies evaluating the effect of heat-stress on the epigenetic profile of bovine oocytes and embryos. Similarly, there is no information on the effect of heat stress on …
An Examination Of Preimplantation Embryos And Endometrium Developed During Induced Aluteal Cycles In The Mare, Chelsey Audra Leisinger
An Examination Of Preimplantation Embryos And Endometrium Developed During Induced Aluteal Cycles In The Mare, Chelsey Audra Leisinger
LSU Doctoral Dissertations
Changes to the embryonic environment that occur within the first few days of development can have significant effects on subsequent fetal development. The following studies investigate the effects of progesterone-deprivation during the early preimplantation period in the mare. The first study described embryo morphology (diameter, stage, and quality grade) collected from mares with induced aluteal cycles. The second study evaluated the effect of induced aluteal cycles on the future fertility of embryo collections as a potential method for cycle manipulation of embryo donor mares. The third study characterized embryonic and endometrial transcript expression of progesterone-mediated transcripts as well as those …
A Path To Success? A Review Of Evolution, Development, And The Predictable Genome By David L. Stern, Hope Hollocher, Charles H. Pence, Grant Ramsey, Michelle M. Wirth
A Path To Success? A Review Of Evolution, Development, And The Predictable Genome By David L. Stern, Hope Hollocher, Charles H. Pence, Grant Ramsey, Michelle M. Wirth
Faculty Publications
No abstract provided.