Developmental Biology Commons^™

The Uas-Gal4 System In D. Melanogaster: An Insight Into The Influence Of Micrornas On The Developmental Pathways Of The Wing, Emily R. Wilson

Honors Theses

By examining genetic pathways in D. melanogaster, a better understanding of the homologous regulatory mechanisms in humans can be utilized to further enhance knowledge of the roles of microRNA within development. This study utilizes the UAS-Gal4 system in order to produce a mutant phenotype capable of being visually studied and analyzed, focusing on the developmental pathway of the wing in D. melanogaster. Dissections of the wandering third instar larvae yielded wing disc tissue expressing the downregulation of loquacious and CG17386.

Impact Of Stress Hormones And Iugr Fetal Conditions On Myoblast Function, Hannah E. Riley, Kristin A. Beede, Dustin T. Yates

UCARE: Research Products

Chronic fetal stress causes adaptive responses that result in intrauterine growth restriction (IUGR). Maternal stressors including heat stress, illness, and obesity cause placental dysfunction that harshens the intrauterine environment by inducing hypoxia and nutrient restriction. IUGR fetuses have restricted growth through the last trimester and after birth. The objective of this study was to test the effects of stress hormones on myoblast proliferation rates. L6 cells and primary myoblasts that were isolated from IUGR fetal sheep in a previous study were used to study the effects of stress hormones on myoblast proliferation and myoblast gene expression. Incubation in epinephrine-spiked media …

An Investigation Of Juvenile Alewife (Alosa Pseudoharengus) Habitat Use And Growth Using Natural Markers, Gregory Norman Labonte Ms

All Student Scholarship

This research attempts to connect patterns in growth and migration of an anadromous species. The goal of this research was to understand habitat movements and growth of juvenile alewives (Alosa pseudoharengus) in the Penobscot Estuary and Bay through the use of otolith microchemistry, otolith growth increments, and a laboratory stable isotope turnover study. Understanding the connection between growth and movement of juvenile alewives may lead to more accurate and sophisticated conservation and restoration methods for anadromous species.

The Reproductive Ecology Of The Timber Rattlesnake, Crotalus Horridus, In Northwestern Arkansas: Interactions Between Environment, Steroid Hormones, And Life History, Craig Michael Lind

Graduate Theses and Dissertations

In this dissertation I examined the relationship between individual energetic status, hormone production, and life history trait expression in field-active Timber Rattlesnakes, Crotalus horridus. In chapter one I reviewed what is known regarding these relationships in snakes and defined major research goals. In chapter two I described the seasonal profile of testosterone (T) and corticosterone (CORT) in relation to the breeding season and to individual energetic status in males. Results showed that the seasonal pattern of T production in C. horridus was different than other pit viper species with similar mating patterns. Testosterone was elevated in the months leading up …

The Development Of Skeletal Muscle In Young Horses: An Ultrasonography And Satellite Cell Approach, Emma K. Lavigne

Honors Scholar Theses

Muscle growth in young horses is characterized by an increase in muscle cross-sectional area, which can be accomplished through the activation and differentiation of satellite cells. Satellite cells can be stimulated or inhibited in response to different cytokines and growth factors and are key mediators of muscle hypertrophy and regeneration. The aim of this study was to investigate the growth of the longissimus dorsi (LD) muscle in horses under 5 years of age and to obtain preliminary data on satellite cell behavior in foals. The area, width, height, and subcutaneous fat were measured using ultrasonography at 6-month increments over the …

Transcriptome Analysis Of Sea Lamprey Embryogenesis, Zakary Ilya Yermolenko

Seton Hall University Dissertations and Theses (ETDs)

The sea lamprey (Petromyzon marinus) has survived throughout evolution for hundreds of millions of years. It is considered an invasive species to the Great Lakes that has caused dramatic changes in the ecosystem for fish communities resulting in the collapse of a fishing industry that was previously valued at billions of dollars. Successful management of the sea lamprey is essential to a sustainable fishing industry and biodiversity. Therefore sea lamprey embryos were studied at various stages of development by growing them in a simulated habitat. RNAs from adult female ovaries and embryos at different time points during embryogenesis …

Protein 14-3-3 (Ywha) Isoforms And Their Roles In Regulating Mouse Oocyte Maturation, Santanu De

Biology Faculty Books and Book Chapters

The 14-3-3 (YWHA) is a highly conserved, ubiquitously expressed protein family regulating important cellular processes including cell cycle. This work, for the first time, explored the differential expression and roles of 14-3-3 isoforms during mouse oocyte maturation.

All seven mammalian 14-3-3 isoforms were identified in mouse eggs and ovarian follicular cells including oocytes, by Western blotting. Immunocytochemical and immunohistochemical staining confirmed the presence of all 14-3-3 isoforms in oocytes, eggs and ovarian follicles with characteristic similarities and differences in their distributions.

Mammalian oocytes are arrested at meiosis prophase I by an inhibitory phosphorylation on Cyclin-Dependent Kinase I (CDK1), released by …

Cartilage On The Move: Cartilage Lineage Tracing During Tadpole Metamorphosis, Ryan R. Kerney, Alison L. Brittain, Brian K. Hall, Daniel R. Buchholz

Biology Faculty Publications

The reorganization of cranial cartilages during tadpole metamorphosis is a set of complex processes. The fates of larval cartilage-forming cells (chondrocytes) and sources of adult chondrocytes are largely unknown. Individual larval cranial cartilages may either degenerate or remodel, while many adult cartilages appear to form de novo during metamorphosis. Determining the extent to which adult chondrocytes/cartilages are derived from larval chondrocytes during metamorphosis requires new techniques in chondrocyte lineage tracing. We have developed two transgenic systems to label cartilage cells throughout the body with fluorescent proteins. One system strongly labels early tadpole cartilages only. The other system inducibly labels forming …

Sex Determination In Beetles: Production Of All Male Progeny By Parental Rnai Knockdown Of Transformer, Jayendra Nath Shukla, Subba Reddy Palli

Entomology Faculty Publications

Sex in insects is determined by a cascade of regulators ultimately controlling sex-specific splicing of a transcription factor, Doublesex (Dsx). We recently identified homolog of dsx in the red flour beetle, Tribolium castaneum (Tcdsx). Here, we report on the identification and characterization of a regulator of Tcdsx splicing in T. castaneum. Two male-specific and one female-specific isoforms of T. castaneum transformer (Tctra) were identified. RNA interference-aided knockdown of Tctra in pupa or adults caused a change in sex from females to males by diverting the splicing of Tcdsx pre-mRNA to male-specific isoform. All the pupa and adults developed from Tctra …

Developing Monitoring Methods For Leptasterias Spp. As Sentinel Species In Detecting Local Environmental Changes, Zachary Sturbaum, Kathryn Nuessly, Riley J. Smith, C. Sarah Cohen

STAR Program Research Presentations

Leptasterias spp., a six-rayed sea star, is found in rocky intertidal habitats ranging from Alaska to central California. Leptasterias spp. can be monitored on a broad scale throughout their range by a variety of means using timed counts and random plot censusing in order to detect both large-scale and local-level changes in the environment due to climate change, land-based human activity, or other environmental events. Leptasterias brood their young externally until the embryos grow into fully developed juveniles. These juveniles disperse by crawling away, limiting their dispersal potential. This localized dispersal provides an opportunity to use Leptasterias spp …

Systematics Of Leptopelis (Anura: Arthroleptidae) From The Itombwe Plateau, Eastern Democratic Republic Of The Congo, Francisco Portillo

Open Access Theses & Dissertations

Leptopelis, a genus of Central African treefrogs, includes 51 species that live in tropical forests and savannas. Currently, only two species of Leptopelis are known from the poorly explored Itombwe Plateau in eastern Democratic Republic of the Congo (DRC). Itombwe is renowned among conservationists for its rich and endemic amphibian fauna, including: Xenopus itombwensis, Chrysobatrachus cupreonitens, Laurentophryne parkeri, Hyperolius leleupi and at least three species of Arthroleptis. Evolutionary relationships of Itombwe Leptopelis were examined by sequencing two mitochondrial genes (16S: 557 bp [base pairs], cyt b: 620 bp) and one nuclear gene (RAG1: 761 bp). Results recovered strong support for …

Metabolic Support Of Anaerobiosis In Embryos Of The Annual Killifish Austrofundulus Limnaeus, Andrew Mccracken

Dissertations and Theses

Embryos of the annual killifish Austrofundulus limnaeus display a remarkable tolerance to anoxia during development, most notably during embryonic diapause. Little is known about the metabolic or enzymatic changes that accompany this state of anoxia tolerance. This study examined the metabolic changes associated with exposure to anoxia by measuring the activity of the enzyme phosphoenolpyruvate carboxykinase (PEPCK), and by profiling the concentration of 31 metabolites ranging from amino acids to citric cycle intermediates at 4 different developmental stages, diapause 2 (DII), 4 days post diapause (dpd), 12 and 22 dpd. Embryos of A. limnaeus showed stage specific changes in concentrations …

The Effects Of Hypoxia And Temperature On Developing Embryos Of The Annual Killifish Austrofundulus Limnaeus, Skye N. Anderson

Dissertations and Theses

Little is known about the physiology or biochemistry of hypoxia (reduced levels of oxygen) tolerance during development in vertebrate embryos. In most species, relatively brief bouts of severe hypoxia are lethal or teratogenic. An exception to such hypoxia intolerance is the annual killifish Austrofundulus limnaeus, in which populations persist in hypoxic environments. This species inhabits seasonal ponds in Venezuela, surviving through the dry season in the form of diapausing embryos. Embedded in the pond sediment, embryos of A. limnaeus are routinely exposed to hypoxia and anoxia (lack of oxygen) as part of their normal development. Here, we exposed embryos to …

Interspecies Comparison Of Αii-Spectrin Abundance Between Chinook Salmon And Steelhead, Brielle D. Kemis, Ann L. Miracle, Katie A. Wagner, Christa M. Woodley

STAR Program Research Presentations

Salmonids, such as Chinook salmon (Oncorhynchus tshawytscha) and steelhead (O. mykiss), are a staple economic, recreational, tribal, and environmental resource, yet many populations are unsustainable. This study was part of a broad scale effort to monitor the impact of downstream migration obstacles on juvenile salmonid health and survival, which is an essential step towards increasing Smolt-to-Adult Return ratios (SARs). The objective of this study was to determine if juvenile Chinook salmon and steelhead exhibit differing quantities of alphaII-Spectrin Breakdown Products (SBDPs) over two consecutive spring migration periods, indicative of neurogenesis rate and/or biological response to head …

Formation Of Intercellular Junctions During Secondary Neurulation In The Tail Bud Region Of Developing Chick Embryos, Selena Mae Losee

Honors Program Theses

During secondary neurulation, the tail bud of the developing chick embryo undergoes a transition from unorganized mesenchymal cells to a structured epithelial tube. Cells undergoing this process must polarize and then assemble specific molecules that allow them to create intercellular junctions. Intercellular junctions contribute to the cells' ability to adhere, communicate with each other, or block the movement of molecules between neighboring cells. Previous studies have suggested cell-to-cell junctions play an important role in cavitation of the tail bud and in organizing the lumina that form. This study aimed to determine if tight junctions and gap junctions develop in this …

The Differential Roles Of D-Pax2 Variants In Regulating Drosophila Eye And Bristle Development, Colin J. O’Shea

Honors Theses

The ability to appropriately interact with the environment is crucial to an organism’s survival. The establishment of functional sensory systems, such as the bristles and eyes in Drosophila, is a critical event during the development of the organism. The transcription factor D Pax2 is involved in the differentiation of the shaft and glial cells in the developing bristle (Kavaler et al., Dev, 126:2261-2272, 1999) and of the cone and primary pigment cells in the developing eye (Fu and Noll, Genes Dev, 11:389-405, 1997). How D-Pax2 contributes to distinct differentiative pathways in different cell types is not known. Recent work by …

Effects Of Calcium Availability On The Reproductive Output Of Insectivorous Bats, Christina M. Booher

All-Inclusive List of Electronic Theses and Dissertations

Reproduction is a period of high calcium demand in vertebrates, and calcium deficiency can limit reproductive output in mammals. Insects are a poor calcium source, suggesting that insectivorous species are more likely to be calcium deficient. Pregnant big brown bats (Eptesicus fuscus) consume 10 times less calcium than they are estimated to require. To accommodate calcium demand during pregnancy, many mammals mobilize more calcium from the skeleton, and extensive bone loss has been observed in some bats during pregnancy. A conflict may arise between the female and developing embryo over allocation of limited calcium supplies, which could limit reproductive output …

Rapid Assimilation Of Yolk Enhances Growth And Development Of Lizard Embryos From A Cold Environment, Melissa Ann Blake

All-Inclusive List of Electronic Theses and Dissertations

Selection for rapid growth in cold environments results in a geographic pattern known as countergradient variation. The eastern fence lizard, Sceloporus undulatus, exhibits countergradient variation in growth rate across latitudinal clines. In a common-garden experiment, S. undulatus embryos from cold environments grew more efficiently than embryos warm environments, even when the energetic contents of eggs were equalized. To examine the physiological mechanisms of higher growth efficiency, I measured the energetics of embryos during the first 49 days of incubation. Specifically, I recorded body mass, residual yolk, total respiration, and developmental stage for embyos from two populations (Virginia and South Carolina). …

Comparing Immune Responses Of Juvenile And Adult Northern Fence Lizards, Sceloporus Undulatus, Zaïna Sandrine Kabange

All-Inclusive List of Electronic Theses and Dissertations

Sex steroid hormones affect behavior and morphological traits important in sexual selection. Steroid hormones also play a role in the development and functioning of immune system components. The immunocompetence handicap hypothesis (Folstad and Karter, 1992) proposes that tradeoffs, mediated by plasma levels of testosterone, exist between the immune system and elaboration of secondary sexual traits. Endocrine-immune interactions are well documented in birds and mammals, and steroid hormones can be immunoenhancing or immunosuppressive. Relatively little work has examined the ontogeny of immune function, and the interactions between steroid hormones and the immune system in reptiles. The first experiment addressed the question …

Thermal Adaptation Of Maternal And Embryonic Phenotypes In A Wide Spread Lizard (Sceloporus Undulatus), Christopher E. Oufiero

All-Inclusive List of Electronic Theses and Dissertations

Geographically widespread ectotherms encounter thermal gradients that generate variation in behavior, physiology and life-history. Because rates of physiological processes are very sensitive to temperature, cold environments are thought to favor genotypes that have relatively high capacities for growth despite environmental constraints. Two different adaptive responses can occur: (1) mothers in colder environments can allocate more energy to each offspring, or (2) offspring in colder environments make more efficient use of available resources. In this study, I examined both maternal adaptations and embryonic adaptations to environmental temperature. Evidence of maternal adaptations was assessed by examining the relationship between egg size and …

Demographic Assessment Of The Blue Crab (Callinectes Sapidus) In Chesapeake Bay Using Extractable Lipofuscins As Age Markers, Se-Jong Ju, David H. Secor, H. Rodger Harvey

OES Faculty Publications

The blue crab (Callinectes sapidus) plays an important economic and ecological role in estuaries and coastal habitats from the Gulf of Mexico to the east coast of North America, but demographic assessments are limited by length-based methods. We applied an alternative aging method using biochemical measures of metabolic byproducts (lipofuscins) sequestered in the neural tissue of eyestalks to examine population age structure. From Chesapeake Bay, subsamples of animals collected from the 1998-99 (n-769) and 1999-2000 (n=367) winter dredge surveys were collected and lipofuscin was measured. Modal analysis of the lipofuscin index provided separation into three modes, whereas carapace-width …

Within-Litter Birth Weight Variation In The Domestic Pig And Its Relation To Pre-Weaning Survival, Weight Gain, And Variation In Weaning Weights, Barry N. Milligan, David Fraser, Donald L. Kramer

Ontogeny Collection

To determine the relationship between within-litter birth weight variation and pre-weaning survival and weight gain, and to provide practical guidance on fostering low-birth-weight piglets, we analyzed piglet survival and weight gain in litters of piglets from 52 sows followed through eight consecutive parities. Litters with high variation in birth weight had more deaths, especially if the litter’s mean birth weight was low. High variation in birth weight was also associated with high variation in weaning weight, but was not significantly related to mean weaning weight. Piglets with birth weights well below the range of most of the litter (‘low-birth-weight piglets’) …

The Effect Of Littermate Weight On Survival, Weight Gain, And Suckling Behavior Of Low-Birth-Weight Piglets In Cross-Fostered Litters, Barry N. Milligan, David Fraser, Donald L. Kramer

Ontogeny Collection

Objective: To determine whether low-birth-weight piglets show better survival, weight gain, and suckling behavior when grouped with other small piglets by cross-fostering.

Methods: We manipulated the number and size of foster littermates for low-birth-weight piglets in 31 small (eight or nine piglets) and 22 large (11 or 12 piglets) litters. Experimental litters were composed of four to six piglets of lowest birth weight and either four to six slightly heavier or four to six much heavier piglets from two combined litters.

Results: Low-birth-weight piglets raised with much heavier littermates had somewhat lower survival rates, but showed no tendency towards lower …

Growth Rate Variability And Lipofuscin Accumulation Rates In The Blue Crab Callinectes Sapidus, Se-Jong Ju, David H. Secor, H. Rodger Harvey

OES Faculty Publications

To better understand growth and age-pigment (lipofuscin) accumulation rates of the blue crab Callinectes sapidus under natural conditions, juveniles (33 to 94 mm carapace width) were reared in outdoor ponds for over 1 yr. Growth rates, measured by carapace width, during summer and fall exceeded all those reported in the literature; the initial carapace width of 59 ± 14 mm (mean ± SD) increased to 164 ± 15 mm within a 3 mo period. No growth occurred during winter months (November to April) at low water temperatures. Growth rates of crabs in ponds were substantially higher (von Bertalanffy growth parameter …

Response Properties Of Tibial Campaniform Sensilla On The Cockroach Leg In Restrained Preparations And Freely Moving Animals, Angela L. Ridgel

Theses, Dissertations and Capstones

The ability to detect changes in forces is important for effective use of a leg in posture and locomotion. This thesis examines how forces are detected in the legs of cockroaches by tibial campaniform sensilla. Campaniform sensilla are mechanoreceptors that encode forces through ovoid cuticular caps embedded in the exoskeleton. The tibial sensilla are unique in that they consist of two subgroups with mutually perpendicular cap orientations.

We characterized the responses of the tibial receptors in restrained preparations by applying forces to the leg at controlled magnitudes and rates. The tibial sensilla, as a group, were sensitive to increasing and …

Estructura Del Condrocráneo Y Esqueleto Visceral De Larvas De Pseudis Minuta (Anura, Pseudidae, E. O. Lavilla, Rafael O. De Sá

Biology Faculty Publications

The chondrocranium and visceral skeleton of Pseudis minuta tadpoles are described, based on a series of five larvae in stages 31 - 35 of Gosner (1960). Among their striking characters are the presence of peculiar articular surfaces between cornua trabeculae and suprarostral cartilage, the incomplete development of the orbital cartilage, the high fenestration of the floor of the cavum cranii, the fusion of posterior foramina, and the fusion of spicules 3 and 4 in the hyobranchial skeleton.

Clones Help Develop Ewe Feeding Strategy, Myra Yelland, Rob Kelly, John Davies, Johan Greeff

Journal of the Department of Agriculture, Western Australia, Series 4

Cloned sheep are not a new animal to the researchers of Agriculture WA, but the method that produced Dolly is.

Cloned sheep have been produced at the great Southern Research Institute as early as the mid 1980s. Myra Yelland, Rob Kelly, John Davies and Johan Greef outline how clones are used in experimental studies on wool production

Vocal Response Of Piglets To Weaning: Effect Of Piglet Age, Daniel M. Weary, David Fraser

Ontogeny Collection

Piglets vocalize a great deal during the first few days after weaning. The aim of this experiment was to determine if the calls given by piglets vary in response to one factor thought to influence post-weaning adaptation: weaning age. In 22 litters, each containing a minimum of 9 piglets, 3 piglets were weaned under identical conditions at 3, 4 and 5 weeks of age. Vocalizations and weight gain were monitored for the week after weaning. The number of calls produced by piglets of all three ages declined from an average of 8.2 calls/min per piglet on the day of weaning …

External Morphology Of The Chorion Of The Annual Fishes Cynolebias (Cyprinodontiformes: Rivulidae), Marcelo Loureiro, Rafael O. De Sá

Biology Faculty Publications

Members of the family Rivulidae (killifishes) inhabit temporary bodies of freshwater in South and Central America (one species is also found in North America). The most remarkable characteristic of the family Rivulidae is that species have an annual life cycle with a drought-resistant egg during the dry season. Parenti's (1981) analysis of the order Cyprinodontiformes considered a single genus, Cynolebias, whereas Cos- ta's (1990) phylogenetic analysis of the family Rivulidae separates Cynolebias from Cynopoecilus. One of Costa's synapomorphies to separate Cynopoecilus is the unique structure of their egg's chorion, which is shared with Leptolebias and Campellolebias.

Postnatal Development Of The Neural Retina In A South American Opossum: Monodelphis Domestica, Tracy L. Soltesz

Theses, Dissertations and Capstones

Postnatal retinal development was studied in a marsupial opossum, Monodelphis domestica using light microscopy and 3H-thymidine autoradiography. For the light microscopic study, opossum neonates at 1, 3, 7, 14, 21, 25 and 28 days of age were euthanized, fixed and processed into paraffin sections for hematoxylin and eosin staining. The distinct ganglion cell layer, first observed on postnatal day (P) 7, was separated from the outer neuroblasts by the inner plexiform layer. The neuroblast layer was divided into inner and outer nuclear layers on P25 by the presumptive outer plexiform layer, indicated by discrete intercellular spaces located between the nuclear …