Cell Biology Commons^™

Detection Of Unique Tumor-Associated Proteins In The Urine Of Patients With Transitional Cell Carcinoma By High Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis, Robert David Lehman

Biological Sciences Theses & Dissertations

A two-dimensional polyacrylamide gel electrophoretic technique was developed for the detection of unique tumor-associated proteins in urine of patients with transitional cell carcinoma (TCC) of the urinary bladder. This technique yielded high resolution of polypeptides in the urine of cancer patients. Urine was chosen as a source of tumor-associated components because the location of the tum or should release already solublized tum or substances in to the urine.

Urinary proteins were concentrated and then analyzed by the two - dimensional electrophoretic technique using isoelectric focusing in the first dimension and sodium dodecylsulfate - polyacrylamide gel electrophoresis in the second dimension. …

A Potential Macroregulatory Mechanism For Growth Regulation In Neurospora Crassa, Martha Mooney

Biological Sciences Theses & Dissertations

Neurospora produces a phase specific, cationic mucopolysaccharide composed primarily of galactosamine (GAG-MP) which becomes part of the cell wall and is later secreted into the media. Its appearance coincides with the onset of the restricted phase of growth and causes efflux of small molecular weight metabolites when incubated with conidial cells. This activity may be a product of electrostatic interactions of the GAG-MP with the conidial plasma membrane. The activity is blocked by acetylation of the primary amines on the molecule, digestion by enzymes that hydrolyze carbohydrate linkages and the inclusion of NaCl (lM) in the GAG-MP/conidia reaction mixture. The …

Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoretic Studies Demonstrating Cytoplasmic Protein Components Unique To The Mycelial Phase And To The Yeast Phase Of Candida Albicans, Theresa Catherine Burgess

Biological Sciences Theses & Dissertations

Blastospore and mycelial cytoplasmic extracts from four strains of Candida albicans, varying in virulence, were compared by sodium dodecyl sulfate (SDS) polyacrylamide disc gel electrophoresis Cytoplasmic extracts were made by disrupting the cultures in a Braun homogenizer followed by ultracentrifugation. The supernatant fractions were then resolved on polyacrylamide gels containing SDS. Each of the preparations yielded a distinct pattern. There were mycelial specific and blastospore specific protein bands. There were more components resolved from the blastospore phase cytoplasmic extracts than from the mycelial phase cytoplasmic extracts. There were bands common to both phases. These results demonstrate protein components unique …

Comparison Of Interferon Produced In Vitro By Monkey Kidney Cells Demonstrating Interference Against Polio Ii, Coxsackie A-9, Influenza A2/Hong Kong/68, And Echo-9 Viruses, Archer Duncan Farmer Jr.

Biological Sciences Theses & Dissertations

Abstract unavailable

Cytogenic Studies Of A Chesapeake Bay Population Of Molgula Manhattensis (Dekay) (Ascidiacea: Stolidobranchia), Elizabeth A. Crawford

Biological Sciences Theses & Dissertations

Abstract unavailable.

The Effects Of The Cyclic 3'5'-Monophosphate Of Adenosine And Its Dibutyryl Derivative On The In-Vitro Immunization Of Mouse Spleen Cell Suspensions, James A. Blanks

Biological Sciences Theses & Dissertations

Abstract unavailable.