Cell Anatomy Commons^™

Embryonic Stem Cells Are Redirected To Non-Tumorigenic Epithelial Cell Fate By Interaction With The Mammary Microenvironment, Corinne A. Boulanger, Robert D. Bruno, David L. Mack, Monica Gonzales, Nadia P. Castro, David S. Salomon, Gilbert H. Smith

School of Medical Diagnostics & Translational Sciences Publications

Experiments were conducted to redirect mouse Embryonic Stem (ES) cells from a tumorigenic phenotype to a normal mammary epithelial phenotype in vivo. Mixing LacZ-labeled ES cells with normal mouse mammary epithelial cells at ratios of 1:5 and 1:50 in phosphate buffered saline and immediately inoculating them into epithelium-divested mammary fat pads of immune-compromised mice accomplished this. Our results indicate that tumorigenesis occurs only when normal mammary ductal growth is not achieved in the inoculated fat pads. When normal mammary gland growth occurs, we find ES cells (LacZ+) progeny interspersed with normal mammary cell progeny in the mammary epithelial structures. We …

A Comparative Study Of The Structure And Origin Of The Yolk Nucleus, John P. Munson

Biology Faculty Scholarship

The question as to the comparative structure and origin of the yolk nucleus involves several other problems:

1. Is the yolk nucleus a normal element of the egg cytoplasm, or is it associated with pathological states of the egg, or is it an artefact due to reagents?

2. Is there a necessary connection between one form and another such that one can be derived from the other?

3. Is it an amorphous chemical substance or does it possess anything suggesting permanence of structure?

4. Does it arise de novo or is it a modification of something similar existing in the …