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Full-Text Articles in Cell Anatomy

Mitochondrial 16s Rrna Gene As A Tool For Scorpion Systematics, Mark D. Barker Jan 2000

Mitochondrial 16s Rrna Gene As A Tool For Scorpion Systematics, Mark D. Barker

Theses, Dissertations and Capstones

The use of morphological analysis in the classification and phylogeny of scorpions often has led to confusion and controversy. In order to clarify these problems other character sets should be considered for analysis. One such tool is the use of molecular comparisons between organisms. This study used a section of the mitochondrial 16S ribosomal RNA gene. This technique was applied to investigate four scorpion genera belonging to three families: Hadruru, Anuroctonus (luridae), Euscorpius (Euscorpiidae), and Centruroides (Buthidae). Total DNA was extracted with a standard phenol/chloroform method; a gene of interest was amplified using PCR; and resulting DNA sequence analysis was …


Electroporation Dynamics In Biological Cells Subjected To Ultrafast Electrical Pulses: A Numerical Simulation Study, R. P. Joshi, K. H. Schoenbach Jan 2000

Electroporation Dynamics In Biological Cells Subjected To Ultrafast Electrical Pulses: A Numerical Simulation Study, R. P. Joshi, K. H. Schoenbach

Bioelectrics Publications

A model analysis of electroporation dynamics in biological cells has been carried out based on the Smoluchowski equation. Results of the cellular response to short, electric pulses are presented, taking account of the growth and resealing dynamics of transient aqueous pores. It is shown that the application of large voltages alone may not be sufficient to cause irreversible breakdown, if the time duration is too short. Failure to cause irreversible damage at small pulse widths could be attributed to the time inadequacy for pores to grow and expand beyond a critical threshold radius. In agreement with earlier studies, it is …