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Full-Text Articles in Cell Anatomy
Regulatory Roles Of Facit Collagens Xii And Xiv In Cornea Stromal And Endothelial Development And Function, Chinda Hemmavanh
Regulatory Roles Of Facit Collagens Xii And Xiv In Cornea Stromal And Endothelial Development And Function, Chinda Hemmavanh
USF Tampa Graduate Theses and Dissertations
Purpose:
Corneal transparency depends upon the precise organization of corneal stromal extracellular matrix and corneal endothelial function. Stromal structure and extracellular matrix organization is responsible for proper refraction of light into the eye. The corneal endothelium is responsible for pumping excess fluid out of the cornea, effectively maintaining corneal hydration and thickness. Corneal transplantation is the current form of treatment for corneal endothelial and stromal dystrophies. The mechanisms controlling stromal collagen fibril packing and organization into orthogonal layers as well as maturation of the endothelium into a fully functioning cellular layer are unknown. Collagens XII and XIV, fibril associated collagens …
Using A Focus Measure To Automate The Location Of Biological Tissue Surfaces In Brightfield Microscopy, Daniel Toby Elozory
Using A Focus Measure To Automate The Location Of Biological Tissue Surfaces In Brightfield Microscopy, Daniel Toby Elozory
USF Tampa Graduate Theses and Dissertations
The study of microstructures in brightfield microscopy using unbiased stereology plays a large and growing role in bioscience research. Stereology enables objective quantitative analysis of biological structures within a tissue sample. A first step in the stereology process is to calculate the thickness of a tissue sample by locating the top and bottom surfaces of the sample. The aim of this project is to fully automate this location process by using the relative optical focus measure as an indicator of tissue surface boundary.
The current method for identification of focus bounding planes requires a trained user to manually select the …