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Full-Text Articles in Cell and Developmental Biology
Role Of Heat Shock Protein 70 Kda Cognate In Limiting Thermal Inactivation And Refolding Of Heat-Denatured Nuclear Type I Topoisomerase, Kuo-Kuang Wen
Role Of Heat Shock Protein 70 Kda Cognate In Limiting Thermal Inactivation And Refolding Of Heat-Denatured Nuclear Type I Topoisomerase, Kuo-Kuang Wen
Theses and Dissertations in Biomedical Sciences
Previous studies (Ciavarra et al., 1994) demonstrated that the constitutive 70 kDa heat shock protein (hsc70) protected purified topoisomerase I from thermal injury. In addition, hsc70 was capable of regenerating catalytic activity of heat-denatured topoisomerase I. A whole cell lysate was also active in this reaction assay. The present study demonstrates that heat-denatured topoisomerase I is reactivated by a cytosolic fraction and that this activity is dependent on the presence of cytosolic hsc70. The efficacy of hsc70-mediated refolding of heat-denatured topoisomerase I is greatly enhanced by a cytosolic cofactor(s). In all these refolding reactions, exogenous ATP is not required. Size …
Induction Of Heat Shock Protein 70 In Chinese Hamster Ovary Cells During Chlamydia Trachomatis Infection, Tsehay Eshete Mekonnen
Induction Of Heat Shock Protein 70 In Chinese Hamster Ovary Cells During Chlamydia Trachomatis Infection, Tsehay Eshete Mekonnen
Theses Digitization Project
No abstract provided.