Open Access. Powered by Scholars. Published by Universities.®
Cell and Developmental Biology Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Cancer Biology (4)
- Biochemistry, Biophysics, and Structural Biology (3)
- Molecular Biology (3)
- Cell Biology (2)
- Analytical, Diagnostic and Therapeutic Techniques and Equipment (1)
-
- Animal Sciences (1)
- Biology (1)
- Biotechnology (1)
- Cell Anatomy (1)
- Immunology and Infectious Disease (1)
- Immunoprophylaxis and Therapy (1)
- International and Area Studies (1)
- Medicine and Health Sciences (1)
- Other Animal Sciences (1)
- Other International and Area Studies (1)
- Social and Behavioral Sciences (1)
- Therapeutics (1)
- Zoology (1)
- Institution
- Publication
- Publication Type
Articles 1 - 9 of 9
Full-Text Articles in Cell and Developmental Biology
Regulation Of The Heat Shock Response And Hsf-1 Nuclear Stress Bodies In C. Elegans, Andrew Deonarine
Regulation Of The Heat Shock Response And Hsf-1 Nuclear Stress Bodies In C. Elegans, Andrew Deonarine
USF Tampa Graduate Theses and Dissertations
The Heat Shock Response (HSR) is a highly conserved stress responsive molecular pathway that functions to promote appropriate protein folding in the cell. The HSR accomplishes this primarily through the use of molecular chaperones that serve to bind to misfolded or unfolded proteins to assist in stabilizing and folding proteins back to their native functional state. The master regulator of this pathway is a transcription factor known as Heat Shock Factor 1 (HSF1). HSF1 regulates molecular chaperone expression in the cell’s basal state, but can also be stress induced by diverse biotic and abiotic signals including thermal shock, oxidative stress, …
Uncovering Transcriptional Activators And Targets Of Hsf-1 In Caenorhabditis Elegans, Jessica Brunquell
Uncovering Transcriptional Activators And Targets Of Hsf-1 In Caenorhabditis Elegans, Jessica Brunquell
USF Tampa Graduate Theses and Dissertations
In order to survive, cells must be able to cope with a variety of environmental stressors. The heat shock response (HSR) is a pro-survival mechanism employed by cells in response to protein denaturing stress, such as heat. Since its discovery in 1960, the heat shock response has been found to be regulated by the transcription factor heat shock factor 1 (HSF1). During periods of increased stress, HSF1 undergoes a multi-step process of activation that involves homotrimerization, DNA-binding, and post-translational regulatory modifications, all of which ultimately function to control the transcription of chaperone genes. These chaperone genes encode molecular chaperone proteins …
Dancing Through Life: Allosteric Transitions And Structural Analysis Of Hsp70 And Hsp110 Chaperone Proteins, Gabrielle Stetz, Gennady M. Verkhivker
Dancing Through Life: Allosteric Transitions And Structural Analysis Of Hsp70 And Hsp110 Chaperone Proteins, Gabrielle Stetz, Gennady M. Verkhivker
Student Scholar Symposium Abstracts and Posters
The molecular chaperone protein Hsp70 is centrally involved in cellular homeostasis by assisting in the folding and degradation of protein substrates. Hsp70 is joined by co-chaperones, such as Hsp110, which contribute to specialized tasks of the Hsp70 complex. Imbalances of this heat shock protein system are believed to be involved with the deregulation of cancer pathways and other human diseases. Better understanding of how these heat shock proteins work at the molecular level, which has been investigated using molecular docking tools, will give more clues about biological function. Simulating the formation and function of Hsp70 based chaperone complexes could provide …
Managing Devil Facial Tumour Disease In Tasmanian Devils (Sarcophilus Harrisii): An Investigation Of Heat Shock Proteins As Potential Vaccine Adjuvants, Monika Payerhin
Managing Devil Facial Tumour Disease In Tasmanian Devils (Sarcophilus Harrisii): An Investigation Of Heat Shock Proteins As Potential Vaccine Adjuvants, Monika Payerhin
Independent Study Project (ISP) Collection
The world’s largest carnivorous marsupial, the Tasmanian devil (Sarcophilus harrisii), is facing extinction from a deadly, highly communicable cancer that has already decimated over 85% of devil populations in the wild: devil facial tumour disease (DFTD). DFTD cells effectively evade recognition by the immune system, and every devil that contracts the disease dies from it. Many attempts have been made at developing a vaccine that could help save this now-threatened species. Heat shock proteins have been linked to enhanced immune recognition of pathogens, making them potential candidates for acting as adjuvants to such a vaccine against DFTD. In this study, …
Cytoplasmic Sequestration Of The Tumor Suppressor P53 By A Heat Shock Protein 70 Family Member, Mortalin, In Human Colorectal Adenocarcinoma Cell Lines, Erin E. Gestl, S. Anne Boettger
Cytoplasmic Sequestration Of The Tumor Suppressor P53 By A Heat Shock Protein 70 Family Member, Mortalin, In Human Colorectal Adenocarcinoma Cell Lines, Erin E. Gestl, S. Anne Boettger
Biology Faculty Publications
No abstract provided.
Mechanism-Based Strategies To Enhance The Actions Of A, Fabiola C. Gomez
Mechanism-Based Strategies To Enhance The Actions Of A, Fabiola C. Gomez
Dissertations & Theses (Open Access)
Heat shock protein 90 (HSP90) is an abundant molecular chaperone that regulates the functional stability of client oncoproteins, such as STAT3, Raf-1 and Akt, which play a role in the survival of malignant cells. The chaperone function of HSP90 is driven by the binding and hydrolysis of ATP. The geldanamycin analog, 17-AAG, binds to the ATP pocket of HSP90 leading to the degradation of client proteins. However, treatment with 17-AAG results in the elevation of the levels of antiapoptotic proteins HSP70 and HSP27, which may lead to cell death resistance. The increase in HSP70 and HSP27 protein levels is due …
The Effects Of 60-Hz Electromagnetic Fields And Teratogens On Drosophila Melanogaster Embryonic Cultures: Analysis Of Heat Shock Proteins 23 And 70, Edmund James Koundakjian
The Effects Of 60-Hz Electromagnetic Fields And Teratogens On Drosophila Melanogaster Embryonic Cultures: Analysis Of Heat Shock Proteins 23 And 70, Edmund James Koundakjian
Theses Digitization Project
No abstract provided.
Role Of Heat Shock Protein 70 Kda Cognate In Limiting Thermal Inactivation And Refolding Of Heat-Denatured Nuclear Type I Topoisomerase, Kuo-Kuang Wen
Role Of Heat Shock Protein 70 Kda Cognate In Limiting Thermal Inactivation And Refolding Of Heat-Denatured Nuclear Type I Topoisomerase, Kuo-Kuang Wen
Theses and Dissertations in Biomedical Sciences
Previous studies (Ciavarra et al., 1994) demonstrated that the constitutive 70 kDa heat shock protein (hsc70) protected purified topoisomerase I from thermal injury. In addition, hsc70 was capable of regenerating catalytic activity of heat-denatured topoisomerase I. A whole cell lysate was also active in this reaction assay. The present study demonstrates that heat-denatured topoisomerase I is reactivated by a cytosolic fraction and that this activity is dependent on the presence of cytosolic hsc70. The efficacy of hsc70-mediated refolding of heat-denatured topoisomerase I is greatly enhanced by a cytosolic cofactor(s). In all these refolding reactions, exogenous ATP is not required. Size …
Induction Of Heat Shock Protein 70 In Chinese Hamster Ovary Cells During Chlamydia Trachomatis Infection, Tsehay Eshete Mekonnen
Induction Of Heat Shock Protein 70 In Chinese Hamster Ovary Cells During Chlamydia Trachomatis Infection, Tsehay Eshete Mekonnen
Theses Digitization Project
No abstract provided.