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Articles 31 - 60 of 193
Full-Text Articles in Biotechnology
Feeding, Nourishing And Medicating An Exploding Global Population: An Argument For Genetic Modification, William A. Price Phd
Feeding, Nourishing And Medicating An Exploding Global Population: An Argument For Genetic Modification, William A. Price Phd
Explorer Café
No abstract provided.
Fatty Acid Transport Protein-2 Inhibitor Grassofermata/Cb5 Protects Cells Against Lipid Accumulation And Toxicity, Nipun Saini, Paul N. Black, David Montefusco, Concetta Dirusso
Fatty Acid Transport Protein-2 Inhibitor Grassofermata/Cb5 Protects Cells Against Lipid Accumulation And Toxicity, Nipun Saini, Paul N. Black, David Montefusco, Concetta Dirusso
Department of Biochemistry: Faculty Publications
The inhibition of the fatty acid uptake into non-adipose tissues provides an attractive target for prevention of lipotoxicity leading to obesity-associated non-alcoholic fatty liver disease and type 2 diabetes. Fatty acid transport proteins (FATPs) are bifunctional proteins involved in the uptake and activation of fatty acids by esterification with coenzyme A. Here we characterize Grassofermata/CB5, previously identified as a fatty acid uptake inhibitor directed against HsFATP2. The compound was effective in inhibiting the uptake of fatty acids in the low micro-molar range (IC50 8–11μM) and prevented palmitate-mediated lipid accumulation and cell death in cell lines that are models for …
Application Of 3d Printing Technology In Porous Anode Fabrication For Enhanced Power Output Of Microbial Fuel Cells, Bin Bian
Electronic Thesis and Dissertation Repository
Microbial fuel cells (MFCs) are widely researched for application in wastewater treatment. However, the current anodes used in MFCs often suffer from high fabrication cost and uncontrollable pore sizes. In this thesis, three-dimensional printing technique was utilized to fabricate anodes with different micro pore sizes for MFCs. Copper coating and carbonization were applied to the printed polymer anodes to increase the conductivity and specific surface area. Voltages of MFCs with various anodes were measured as well as other electrochemical tests such as linear sweep voltammetry and electrochemical impedance spectroscopy. 3D copper porous anode produced higher maximum voltages and power densities …
Characterizing The Rogfp2-Orp1 Fluorescent Biosensor For Detecting Oxidative Stress In Mammalian Cells, Sara A. Doan, Stevie Norcross, Mathew Tantama
Characterizing The Rogfp2-Orp1 Fluorescent Biosensor For Detecting Oxidative Stress In Mammalian Cells, Sara A. Doan, Stevie Norcross, Mathew Tantama
The Summer Undergraduate Research Fellowship (SURF) Symposium
Parkinson’s disease is a neurodegenerative disease involving the death of neurons in the substantia nigra and loss of the neurotransmitter, dopamine. The disease leads to progressive loss of motor control. Exact causes and mechanisms by which Parkinson’s disease proceeds are unknown, however, previous experiments determine oxidative stress in mitochondria as a factor that results in cell death. Strategies have been implemented to generate fluorescent biosensors to monitor reactive oxygen species (ROS) concentrations while simultaneously measuring the spatiotemporal distribution and correlation between the ROS, cellular function and organelle. Orp1, an enzyme found in yeast, is a sensitive oxidizing species and when …
A Plant-Made Vaccine Candidate To Protect Ruminants Against Shiga Toxin-Producing Escherichia Coli, Sean Miletic
A Plant-Made Vaccine Candidate To Protect Ruminants Against Shiga Toxin-Producing Escherichia Coli, Sean Miletic
Electronic Thesis and Dissertation Repository
Shiga toxin-producing Escherichia coli (STEC) are enteropathogens colonizing the digestive tracts of humans and animals worldwide. STEC are shed in the manure of cattle and frequently contaminate beef products, vegetables, and drinking water. Immunizing cattle herds against STEC is a promising strategy to reduce STEC colonization in cattle and therefore decrease contamination in the food supply. The goal of this project is to produce a plant-made vaccine to protect ruminants against STEC. Several recombinant proteins were designed as vaccine candidates and expressed transiently in Nicotiana benthamiana and transplastomically Nicotiana tabacum. One vaccine candidate, an E. coli secreted protein A (EspA) …
Short Peptides In Minimalistic Biocatalyst Design, Krystyna L. Duncan, Rein V. Ulijn
Short Peptides In Minimalistic Biocatalyst Design, Krystyna L. Duncan, Rein V. Ulijn
Publications and Research
We review recent developments in the use of short peptides in the design of minimalistic biocatalysts focusing on ester hydrolysis. A number of designed peptide nanostructures are shown to have (modest) catalytic activity. Five features are discussed and illustrated by literature examples, including primary peptide sequence, nanosurfaces/scaffolds, binding pockets, multivalency and the presence of metal ions. Some of these are derived from natural enzymes, but others, such as multivalency of active sites on designed nanofibers, may give rise to new features not found in natural enzymes. Remarkably, it is shown that each of these design features give rise to similar …
The Cababc Operon Essential For Biofilm And Rugose Colony Development In Vibrio Vulnificus, Jin Hwan Park, Youmi Jo, Song Yee Jang, Haenaem Kwon, Yasuhiko Irie, Matthew R. Parsek, Myung Hee Kim, Sang Ho Choi
The Cababc Operon Essential For Biofilm And Rugose Colony Development In Vibrio Vulnificus, Jin Hwan Park, Youmi Jo, Song Yee Jang, Haenaem Kwon, Yasuhiko Irie, Matthew R. Parsek, Myung Hee Kim, Sang Ho Choi
Biology Faculty Publications
A transcriptome analysis identified Vibrio vulnificus cabABC genes which were preferentially expressed in biofilms. The cabABC genes were transcribed as a single operon. The cabA gene was induced by elevated 3′,5′-cyclic diguanylic acid (c-di-GMP) and encoded a calcium-binding protein CabA. Comparison of the biofilms produced by the cabA mutant and its parent strain JN111 in microtiter plates using crystal-violet staining demonstrated that CabA contributed to biofilm formation in a calcium-dependent manner under elevated c-di-GMP conditions. Genetic and biochemical analyses revealed that CabA was secreted to the cell exterior through functional CabB and CabC, distributed throughout the biofilm matrix, and produced …
Drosophila Eye Model To Study Neuroprotective Role Of Creb Binding Protein (Cbp) In Alzheimer’S Disease, Timothy Cutler, Ankita Sarkar, Michael Moran, Andrew Steffensmeier, Oorvashi Roy Puli, Greg Mancini, Meghana Tare
Drosophila Eye Model To Study Neuroprotective Role Of Creb Binding Protein (Cbp) In Alzheimer’S Disease, Timothy Cutler, Ankita Sarkar, Michael Moran, Andrew Steffensmeier, Oorvashi Roy Puli, Greg Mancini, Meghana Tare
Biology Faculty Publications
Background: The progressive neurodegenerative disorder Alzheimer’s disease (AD) manifests as loss of cognitive functions, and finally leads to death of the affected individual. AD may result from accumulation of amyloid plaques. These amyloid plaques comprising of amyloid-beta 42 (Aβ42) polypeptides results from the improper cleavage of amyloid precursor protein (APP) in the brain. The Aβ42 plaques have been shown to disrupt the normal cellular processes and thereby trigger abnormal signaling which results in the death of neurons. However, the molecular-genetic mechanism(s) responsible for Aβ42 mediated neurodegeneration is yet to be fully understood.
Methodology/Principal Findings: We have utilized Gal4/UAS system to …
Evaluation Of Phenotyping And Genotyping Characteristic Of Shigella Sonnei After Biofield Treatment, Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Khemraj Bairwa, Snehasis Jana
Evaluation Of Phenotyping And Genotyping Characteristic Of Shigella Sonnei After Biofield Treatment, Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Khemraj Bairwa, Snehasis Jana
Mahendra Kumar Trivedi
Effect Of Biofield Treatment On Phenotypic And Genotypic Characteristic Of Provindencia Rettgeri, Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Khemraj Bairwa, Snehasis Jana
Effect Of Biofield Treatment On Phenotypic And Genotypic Characteristic Of Provindencia Rettgeri, Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Khemraj Bairwa, Snehasis Jana
Mahendra Kumar Trivedi
Design And Synthesis Of Polymeric Nanoparticles For Drug And Protein Delivery, Judy A. Ventura
Design And Synthesis Of Polymeric Nanoparticles For Drug And Protein Delivery, Judy A. Ventura
Doctoral Dissertations
Nanoparticles are emerging as carriers in biological applications due to advances in their preparation, size control, surface modification and encapsulation capabilities. In addition, nanomaterials improve bioavailability by enhancing aqueous solubility of the guest molecule and increasing resistance time in the body. However, the delivery of guest molecules is still challenging due to the intrinsic characteristics of the guest molecule including large size and propensity to denature or degradation in the case of biomolecules and the encapsulation stability of the small guest molecules. Our group recently reported the preparation of self-cross-linked polymeric nanogels possessing surface functionalization capabilities. In this dissertation we …
Engineering Novel Detection And Treatment Strategies For Bacterial Therapy Of Cancer, Jan T. Panteli
Engineering Novel Detection And Treatment Strategies For Bacterial Therapy Of Cancer, Jan T. Panteli
Doctoral Dissertations
Finding and treating cancer is difficult due to limited sensitivity and specificity of current detection and treatment strategies. Many chemotherapeutic drugs are small molecules that are limited by diffusion, making it difficult to reach cancer sites requiring high doses that lead to systemic toxicity and off-target effects. Tomographic detection techniques, like PET, MRI and CT, are good at identifying macroscopic lesions in the body but are limited in their ability to detect microscopic lesions. Biomarker detection strategies are extremely sensitive and able to identify ng/ml concentrations of protein, but are poor at discriminating between healthy and disease state levels due …
Transferrin-Based Therapeutics And Analytical Methods To Characterize Them, Son N. Nguyen
Transferrin-Based Therapeutics And Analytical Methods To Characterize Them, Son N. Nguyen
Doctoral Dissertations
Modern development of medicine requires detailed characterization by state-of-the art analytical techniques that can be used to analyze covalent structure, conformations and protein-receptor interaction to quantitatively measure biodistribution of protein therapeutics. Mass spectrometry has already become an indispensable tool facilitating all stages of protein drug development. Particularly, this work has demonstrated the tremendous potential of electrospray ionization (ESI) mass spectrometry (MS) in this arena by providing invaluable information beyond mass measurement that can be used to optimize protein drug conjugate structures during early stages of development, and to further catalyze drug design efforts. Additionally, a new sensitive and selective method …
The Application Of The Ultrafine Technology In Improving The Biocompatibility And Osteo-Inductivity Of Dental Implants, Nicholas Y. Hou
The Application Of The Ultrafine Technology In Improving The Biocompatibility And Osteo-Inductivity Of Dental Implants, Nicholas Y. Hou
Electronic Thesis and Dissertation Repository
Dental implants are very effective medical devices. However, although stable, the conventional titanium implants are not very bioactive which in some instances could reduce their efficacy. This thesis described the research progress of using polymeric ultrafine-particles with bioactive additives to enrich the surface of titanium substrate, thereby increasing the biocompatibility and osteo-inductivity of the biomaterial. During this doctoral project, three different types of polymers were examined, initially the conventional polyester, and later the novel epoxy as well as the epoxy/polyester hybrid polymers. Physical characterizations confirmed that all of the coating powders were ultrafine particles, and homogeneous surfaces were constructed from …
Considerations Of Potential Tort Liability With Respect To Natural Draft Cooling Towers Associated With Steam-Electric Power Plants, Thomas D. Corkran
Considerations Of Potential Tort Liability With Respect To Natural Draft Cooling Towers Associated With Steam-Electric Power Plants, Thomas D. Corkran
Akron Law Review
To prevent thermal pollution and to conserve our water supply, it appears that we must learn to live with natural draft cooling towers, at least for the next several decades. Proponents of natural draft cooling towers maintain that the possibility of localized fogging and icing is negligible, but the potential hazards of artificial salt fallout are very real. Also, there appears to be a trend developing in the law which could lead to an action against the operator of a natural draft cooling tower for aesthetic annoyances. There are several theories of action which might lie in such cases, but …
Understanding The Backlog Problems Associated With Requests For Continued Examination Practice, Sean Tu
Understanding The Backlog Problems Associated With Requests For Continued Examination Practice, Sean Tu
Duke Law & Technology Review
One of the greatest problems facing the current patent administration is a long patent pendency period. This study focuses on Request for Continued Examination (RCE) practice, and its effects on the current patent application backlog problem. RCEs are used to continue prosecution after a patent examiner has issued a final rejection. However, now that RCEs are placed on an examiner’s special docket, some examiners may pick up prosecution one to two years after the last action. Accordingly, there are great inefficiencies that may be created by this delay, such as relearning issues and questions from the previous action, diminished value …
Role Of Macrophages In Muscle Transfection With Pdna/Pluronic Formulation, Vivek Mahajan
Role Of Macrophages In Muscle Transfection With Pdna/Pluronic Formulation, Vivek Mahajan
Theses & Dissertations
Non-ionic amphiphilic block copolymers of poly(ethylene oxide) (PEO) and poly(propylene oxide) (PPO), Pluronics, arranged in a tri-block structure PEO-PPO-PEO, have raised a considerable interest in skeletal muscle Gene Therapy. Previous studies have demonstrated that co-administration of Pluronics with naked plasmid DNA (pDNA) by direct i.m. injection enhanced transgene expression not only in muscle but also in distal lymphoid organs (spleen and lymph nodes) and this response was strain-dependent; not observed in athymic (BALB/c nu/nu) mouse; suggesting a role of immune cells in gene transfer to skeletal muscles. Therefore, we first evaluated the role of inflammation and inflammatory cells, on muscle …
Examining The Nucleotide Preference Of The Linker Domain In Engineered Tev-Mtalens, Brendon C. Mcdowell
Examining The Nucleotide Preference Of The Linker Domain In Engineered Tev-Mtalens, Brendon C. Mcdowell
Electronic Thesis and Dissertation Repository
Tev-mTALENs are genome-editing nucleases which combine the nuclease and linker domains of I-TevI with the DNA-binding domain of a TAL effector. The linker domain interacts with a portion of the Tev-mTALEN target site called the DNA Spacer, facilitating DNA cleavage. Linker-DNA Spacer interactions are poorly understood but necessary for Tev-mTALEN activity. I examined the DNA Spacer sequence requirements of the linker by assaying Tev-mTALEN activity on targets with mutated DNA Spacer sequences. I also performed activity assays using Tev-mTALENs with mutations to the I-TevI linker domain. My results indicate that the linker DNA Spacer sequence requirements are highly cryptic. No …
Potential Impact Of Biofield Treatment On Atomic And Physical Characteristics Of Magnesium, Mahendra Kumar Trivedi, Rama Mohan Tallapragada, Alice Branton, Dahryn Trivedi, Gopal Nayak, Omprakash Latiyal, Snehasis Jana
Potential Impact Of Biofield Treatment On Atomic And Physical Characteristics Of Magnesium, Mahendra Kumar Trivedi, Rama Mohan Tallapragada, Alice Branton, Dahryn Trivedi, Gopal Nayak, Omprakash Latiyal, Snehasis Jana
Mahendra Kumar Trivedi
Magnetic Nanoparticles Containing Labeling Reagents For Cell Surface Mapping, Ujwal S. Patil
Magnetic Nanoparticles Containing Labeling Reagents For Cell Surface Mapping, Ujwal S. Patil
University of New Orleans Theses and Dissertations
Cell surface proteins play an important role in understanding cell-cell communication, cell signaling pathways, cell division and molecular pathogenesis in various diseases. Commonly used biotinylation regents for cell surface mapping have shown some potential drawbacks such as crossing the cell membrane, difficult recovery of biotinylated proteins from streptavidin/avidin beads, interference from endogenous biotin and nonspecific nature of streptavidin. With aim to solve these problems, we introduced sulfo-N-hydroxysuccinimidyl (NHS) ester functionalized magnetic nanoparticles containing cleavable groups to label solvent exposed primary amine groups of proteins. Silica coated iron oxide magnetic nanoparticles (Fe3O4@SiO2 MNPs) were …
Biofield Treatment: A Potential Strategy For Modification Of Physical And Thermal Properties Of Indole, Mahendra Kumar Trivedi, Rama Mohan Tallapragada, Alice Branton, Dahryn Trivedi, Gopal Nayak, Rakesh K. Mishra, Snehasis Jana
Biofield Treatment: A Potential Strategy For Modification Of Physical And Thermal Properties Of Indole, Mahendra Kumar Trivedi, Rama Mohan Tallapragada, Alice Branton, Dahryn Trivedi, Gopal Nayak, Rakesh K. Mishra, Snehasis Jana
Mahendra Kumar Trivedi
Development Of A Novel Enzymatic Pre-Treatment For Lignocellulosic Biomass, Melissa Robins, Jenna Rickus
Development Of A Novel Enzymatic Pre-Treatment For Lignocellulosic Biomass, Melissa Robins, Jenna Rickus
The Summer Undergraduate Research Fellowship (SURF) Symposium
Biofuels, fuels derived directly from living matter, present a renewable and environmentally friendly alternative to petroleum based fuels. Bioethanol produced from low input energy crops or agricultural waste is a promising fuel source because it does not interfere with the human food supply chain and the ethanol produced can be blended with gasoline. These potential sources of bioethanol are not yet commercially viable due to a polymer called lignin present in the plant’s cell wall which impedes the conversion of cellulose to glucose and the eventual fermentation of glucose to ethanol. Developing new methods for the pretreatment of lignocellulosic biomass …
Bio-Inspired Composite Hydrogels For Osteochondral Regenerative Engineering, Grant N. Gellert, Liangju Kuang, Chunhui Jiang, Nur P. Damayanti, Joseph Irudayaraj, Meng Deng
Bio-Inspired Composite Hydrogels For Osteochondral Regenerative Engineering, Grant N. Gellert, Liangju Kuang, Chunhui Jiang, Nur P. Damayanti, Joseph Irudayaraj, Meng Deng
The Summer Undergraduate Research Fellowship (SURF) Symposium
Treatment of osteochondral defects encompassing injury or degeneration to both the articular cartilage as well as the underlying subchondral bone presents a significant medical challenge. Current treatment options including autografts and allografts suffer from limited availability and risk of immunogenicity, respectively. The long term goal of this work is to develop an integrated scaffold system for treatment of osteochondral defects via in situ regeneration of bone, cartilage and the bone-cartilage interface. Hydrogels composed of polymer networks swollen in water provide an attractive biomaterial platform for regeneration of cartilage. In the present study, we have developed a novel composite hydrogel consisting …
A Novel Synthetic Yeast For Enzymatic Biodigester Pretreatment, Tianyu Tan, Mark S. Aronson, Arren Liu, Jill H. Osterhus, Melissa Robins, Suraj Mohan, Erich Leazer, Bowman Clark, Alexa Petrucciani, Katherine Lowery, James Welch, Casey Martin, Helena Lysandrou, Michael E. Scharf, Jenna Rickus
A Novel Synthetic Yeast For Enzymatic Biodigester Pretreatment, Tianyu Tan, Mark S. Aronson, Arren Liu, Jill H. Osterhus, Melissa Robins, Suraj Mohan, Erich Leazer, Bowman Clark, Alexa Petrucciani, Katherine Lowery, James Welch, Casey Martin, Helena Lysandrou, Michael E. Scharf, Jenna Rickus
The Summer Undergraduate Research Fellowship (SURF) Symposium
Lignin, a complex organic polymer, is a major roadblock to the efficiency of biofuel conversion as it both physically blocks carbohydrate substrates and poisons biomass degrading enzymes, even if broken down to monomer units. A pretreatment process is often applied to separate the lignin from biomass prior to biofuel conversion. However, contemporary methods of pretreatment require large amounts of energy, which may be economically uncompelling or unfeasible. Taking inspiration from several genes that have been isolated from termites and fungi which translate to enzymes that degrade lignin, we want to establish a novel “enzymatic pretreatment” system where microbes secrete these …
Impact Of Biofield Treatment On Atomic And Structural Characteristics Of Barium Titanate Powder, Mahendra Kumar Trivedi, Gopal Nayak, Shrikant Patil, Rama Mohan Tallapragada, Omprakash Latiyal, Snehasis Jana
Impact Of Biofield Treatment On Atomic And Structural Characteristics Of Barium Titanate Powder, Mahendra Kumar Trivedi, Gopal Nayak, Shrikant Patil, Rama Mohan Tallapragada, Omprakash Latiyal, Snehasis Jana
Mahendra Kumar Trivedi
An Evaluation Of Biofield Treatment On Thermal, Physical And Structural Properties Of Cadmium Powder, Mahendra Kumar Trivedi, Gopal Nayak, Shrikant Patil, Rama Mohan Tallapragada, Omprakash Latiyal, Snehasis Jana
An Evaluation Of Biofield Treatment On Thermal, Physical And Structural Properties Of Cadmium Powder, Mahendra Kumar Trivedi, Gopal Nayak, Shrikant Patil, Rama Mohan Tallapragada, Omprakash Latiyal, Snehasis Jana
Mahendra Kumar Trivedi
In Vitro Evaluation Of Biofield Treatment On Cancer Biomarkers Involved In Endometrial And Prostate Cancer Cell Lines, Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Mayank Gangwar, Snehasis Jana
In Vitro Evaluation Of Biofield Treatment On Cancer Biomarkers Involved In Endometrial And Prostate Cancer Cell Lines, Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Mayank Gangwar, Snehasis Jana
Mahendra Kumar Trivedi
Engineering Photosystem I Complexes For Use In Bio-Hybrid Dye-Sensitized Solar Cells, Richard Franklin Simmerman
Engineering Photosystem I Complexes For Use In Bio-Hybrid Dye-Sensitized Solar Cells, Richard Franklin Simmerman
Doctoral Dissertations
Increasing global population, growing per capita energy needs, diminishing fossil fuels, and climate change collectively will require new, innovative, and sustainable alternatives to meet the world’s growing energy needs. One of the most promising yet simple approaches are dye-sensitized solar cells (DSSCs). However, conventional DSSCs use semi-conductor anodes sensitized with complex synthetic organometallic dyes. Most dyes utilize ruthenium complexes to absorb photons, which upon excitation, inject electrons into the anode, while holes migrate to the cathode via liquid electrolyte. However, these dyes are expensive, difficult to make, and resource-limited. This dissertation focuses on replacing synthetic dyes with the naturally occurring, …
Genetic Modification Of Switchgrass (Panicum Virgatum L.) For Improvement Of Plant Architecture, Biomass Productivity And Sugar Release Efficiency For Biofuel, Wegi Aberra Wuddineh
Genetic Modification Of Switchgrass (Panicum Virgatum L.) For Improvement Of Plant Architecture, Biomass Productivity And Sugar Release Efficiency For Biofuel, Wegi Aberra Wuddineh
Doctoral Dissertations
Switchgrass (Panicum virgatum L.) is a leading candidate bioenergy crop for sustainable biofuel production. To ensure its economic viability, tremendous improvements in switchgrass biomass productivity and recalcitrance to enzymatic saccharification are needed. Genetic manipulation of lignin biosynthesis by targeting transcriptional regulators of higher level domains of lignin biosynthesis and other complex traits could alter several bioenergy-desirable traits at once. A three-pronged approach was made in the dissertation research to target one plant growth regulator and transcription factors to alter plant architecture and cell wall biosynthesis.
Gibberellin (GA) catabolic enzymes, GA 2-oxidases (GA2oxs), were utilized to alternatively modify the lignin …
Prevalence Of A Chytrid Pathogen (Batrachochytrium Dendrobatidis) In Eastern Hellbender Salamanders In New York And Pennsylvania, Linxuan Wu
Biology Theses
Amphibian populations are currently declining globally. There are many possible causes for these declines, among which an emerging infectious disease, chytridiomycosis, has been implicated. Chytridiomycosis in the U.S.A. is mainly caused by the Batrachochytrium dendrobatidis. In this study, I used qPCR assays to detect the existence of this pathogen in the Eastern Hellbender (Cryptobranchus alleganiensis alleganiensis) populations in the Allegheny and Susquehanna River drainages of New York and Pennsylvania. Chytrid is most often tested by using skin swabs, but in this study, tail clips, dorsal skin, blood and eggs were tested as well. Batrachochytrium dendrobatidis was detected …