Biotechnology Commons^™

Use Of Co-Immunoprecipitations And 2d Gel Electrophoresis To Identify Protein-Protein Interactions Of Maturase K, Lauren Angello

Honors Theses

Maturase K (MatK) is the only group II intron encoded protein in the chloroplast of land plants. Maturases are prokaryotic enzymes that catalyze formation of the lariat structure needed for intron removal from precursor RNAs. The chloroplast maturase MatK, is a descendant of prokaryotic maturases, however, unlike its prokaryotic relatives, MatK is thought to catalyze excision of, not only its own intron, but also the introns of other group II introns in the plastome. Similar to the multiprotein and snRNA spliceosomal complex of the nucleus, it is postulated that MatK is not working alone to excise these introns but most …

Plant And Microbial Responses To Repeated Cu(Oh)2 Nanopesticide Exposures Under Different Fertilization Levels In An Agro-Ecosystem, Marie Simonin, Benjamin P. Colman, Weiyi Tang, Jonathan D. Judy, Steven M. Anderson, Christina M. Bergemann, Jennifer D. Rocca, Jason M. Unrine, Nicolas Cassar, Emily S. Bernhardt

Plant and Soil Sciences Faculty Publications

The environmental fate and potential impacts of nanopesticides on agroecosystems under realistic agricultural conditions are poorly understood. As a result, the benefits and risks of these novel formulations compared to the conventional products are currently unclear. Here, we examined the effects of repeated realistic exposures of the Cu(OH)₂ nanopesticide, Kocide 3000, on simulated agricultural pastureland in an outdoor mesocosm experiment over 1 year. The Kocide applications were performed alongside three different mineral fertilization levels (Ambient, Low, and High) to assess the environmental impacts of this nanopesticide under low-input or conventional farming scenarios. The effects of Kocide over time were …

Lipid Transport Required To Make Lipids Of Photosynthetic Membranes, Evan Labrant, Allison C. Barnes, Rebecca Roston

Department of Biochemistry: Faculty Publications

Photosynthetic membranes provide much of the usable energy for life on earth. To produce photosynthetic membrane lipids, multiple transport steps are required, including fatty acid export from the chloroplast stroma to the endoplasmic reticulum, and lipid transport from the endoplasmic reticulum to the chloroplast envelope membranes. Transport of hydrophobic molecules through aqueous space is energetically unfavorable and must be catalyzed by dedicated enzymes, frequently on specialized membrane structures. Here, we review photosynthetic membrane lipid transport to the chloroplast in the context of photosynthetic membrane lipid synthesis. We independently consider the identity of transported lipids, the proteinaceous transport components, and membrane …

Assessment Of Resistance To Botrytis Cinerea In Arabidopsis Expressing Grapevine Sts Genes And Analysis Of New Grapevine Vein Clearing Virus Isolates, Li Su

Graduate Theses/Dissertations

Grapevine stilbene synthase gene (STS) family is unusually large with more than 32 members of full-length genes. Resveratrol and piceid are synthesized in VaSTS7-, VvSTS22-, VaSTS22-transgenic Arabidopsis. To study the function of these STS genes in the resistance against Botrytis cinerea in vivo, lesion sizes on these STS transgenic Arabidopsis were compared to that on non-transgenic control Col-0, at 96 hours post inoculation with B. cinerea spores at 0, 200, or 1,000 spores/μl. In each experiment there were three biological replicates. Three lesion sizes from each replicate were averaged. The experiment was repeated three …

Keragaan Tiga Jenis Planlet Anggrek Phalaenopsis Asal Protocorm Yang Diinduksi Ethyl Methyl Sulfonate (Ems) Secara In Vitro, Romiyadi, Ai Komariah, Suseno Amien

Jurnal Kultivasi

This research was conducted to determine the effect of concentration of Ethyl Methyl Sulfonate (EMS) to performance of three types of Phalaenopsis Planlets and to know the optimum concentration of EMS on any type of Phalaenopsis orchids. The experiment was conduc-ted at Tissue Culture Laboratory of the Faculty of Agriculture, Winaya Mukti University, Sumedang. It used a completely randomized design (CRD) with factorial pattern, that consisted of two factors and replicated twice.The first factor was the type of Phalaenopsis that resulted form hybridization which consisted of v1 (Phalaenopsis 717 X Phalae-nopsis Fire Bird), v2 (Phalaenopsis Tianong Rose X Sibling), and …

Chitosan Biopolymer Promotes Yield And Stimulates Accumulation Of Antioxidants In Strawberry Fruit, Mosaddiqur Rahman, Julakha Akter Mukta, Abdullah As Sabir, Dipall Rani Gupta, Mohammed Mohi-Ud-Din, Mirza Hasanuzzaman, Md. Giashuddin Miah, Mahfuzur Rahman, Md Tofazzai Islam

Faculty & Staff Scholarship

Strawberry is a well-known source of natural antioxidants with excellent free radical scav- enging capacity. This study determined the effects of chitosan application in field condition on plant growth, fruit yield and antioxidant activities in strawberry fruit. Foliar applications of chitosan on strawberry significantly increased plant growth and fruit yield (up to 42% higher) compared to untreated control. Increased fruit yield was attributed to higher plant growth, individual fruit weight and total fruit weight/plant due to the chitosan application. Surprisingly, the fruit from plants sprayed with chitosan also had significantly higher contents (up to 2.6- fold) of carotenoids, anthocyanins, flavonoids …

Pleiotropic And Epistatic Network-Based Discovery: Integrated Networks For Target Gene Discovery, Deborah Weighill, Piet Jones, Manesh Shah, Priya Ranjan, Wellington Muchero, Jeremy Schmutz, Avinash Sreedasyam, David Macaya-Sanz, Robert Sykes, Nan Zhao, Madhavi Z. Martin, Stephen Difazio, Timothy J. Tschaplinski, Gerald Tuskan, Daniel Jacobson

Faculty & Staff Scholarship

Biological organisms are complex systems that are composed of functional networks of interacting molecules and macro-molecules. Complex phenotypes are the result of orchestrated, hierarchical, heterogeneous collections of expressed genomic variants. However, the effects of these variants are the result of historic selective pressure and current environmental and epigenetic signals, and, as such, their co-occurrence can be seen as genome-wide correlations in a number of different manners. Biomass recalcitrance (i.e., the resistance of plants to degradation or deconstruction, which ultimately enables access to a plant’s sugars) is a complex polygenic phenotype of high importance to biofuels initiatives. This study makes use …

Structure Of The Ambrosia Beetle (Coleoptera: Curculionidae) Mycangia Revealed Through Micro-Computed Tomography, You Li, Yongying Ruan, Matthew T. Kasson, Edward L. Stanley, Conrad P.D.T Gillett, Andrew J. Johnson, Mengna Zhang, Jiri Hulcr

Faculty & Staff Scholarship

Ambrosia beetles (Coleoptera: Curculionidae: Scolytinae and Platypodinae) rely on a symbiosis with fungi for their nutrition. Symbiotic fungi are preserved and transported in specialized storage structures called mycangia. Although pivotal in the symbiosis, mycangia have been notoriously difficult to study, given their minute size and membranous structure. We compared the application of novel visualization methods for the study of mycangia, namely micro-computed tomography (micro-CT) and laser ablation tomography (LATscan) with traditional paraffin sectioning. Micro-CT scanning has shown the greatest promise in new organ discovery, while sectioning remains the only method with sufficient resolution for cellular visualization. All three common types …

Plant Probiotic Bacteria Bacillus And Paraburkholderia Improve Growth, Yield And Content Of Antioxidants In Strawberry Fruit, Mosaddiqur Rahman, Abdullah As Sabir, Julakha Akter Mukrta, Mohibul Alam Khan, Mohammed Mohi-Ud-Din, Giashuddin Miah, Mahfuzur Rahman, M. Tofazzal Islam

Faculty & Staff Scholarship

Strawberry is an excellent source of natural antioxidants with high capacity of scavenging free radicals.

This study evaluated the effects of two plant probiotic bacteria, Bacillus amylolequefaciens BChi1 and Paraburkholderia fungorum BRRh-4 on growth, fruit yield and antioxidant contents in strawberry fruits. Root dipping of seedlings (plug plants) followed by spray applications of both probiotic bacteria in the field on foliage significantly increased fruit yield (up to 48%) over non-treated control. Enhanced fruit yield likely to be linked with higher root and shoot growth, individual and total fruit weight/plant and production of phytohormone by the probiotic bacteria applied on plants. …

Multiplikasi Tunas Mikro Pisang (Musa Paradisiaca L.) ‘Raja Bulu’ Secara In Vitro Pada Berbagai Jenis Dan Konsentrasi Sitokinin, Tiara Elma, Erni Suminar, Syariful Mubarok, Anne Nuraini

Jurnal Kultivasi

Terbatasnya ketersediaan bibit pisang bermutu di Indonesia disebabkan oleh masih rendahnya jumlah dan kualitas bibit pisang yang dihasilkan melalui metode konvensional. Waktu yang dibutuhkan untuk mendapatkan bibit cukup lama, oleh karena itu digunakan suatu metode perbanyakan untuk menghasilkan bibit dalam waktu yang relatif singkat melalui metode kultur jaringan. Tujuan dari penelitian ini adalah untuk mendapatkan salah satu jenis dan konsentrasi sitokinin terbaik dalam meningkatkan laju multiplikasi tunas pisang raja bulu secara in vitro. Percobaan dilaksanakan dari bulan November 2016 sampai bulan Maret 2017 di Laboratorium Kultur Jaringan dan Teknologi Benih Fakultas Pertanian Universitas Padjadjaran di Jatinangor, Sumedang. Rancangan Acak Lengkap …

Advanced Biotechnology Tools For Invasive Species Management, Invasive Species Advisory Committee

National Invasive Species Council

Increasingly, genetic tools are being used to detect and solve pressing environmental, social, and health-related challenges. It is clear that investments in technology innovation can be game changing, as advances in biotechnology may provide new methods to protect the nation’s resources from the negative impacts of invasive species. The current toolbox of management options is recognizably insufficient to deal with many of the high-impact species that have been introduced. However, “surrendering” to these species is generally not a viable option from ecological, health, economic, socio-cultural, or political perspectives. Cost-efficient solutions to these “grand invasive species challenges” need to be found. …

Pembentukan Ubi Mikro Kentang (Solanum Tuberosum L.) Pada Berbagai Komposisi Media In Vitro, Inneke Amalia, Anne Nuraini, Sumadi, Syariful Mubarok, Erni Suminar

Jurnal Kultivasi

Permasalahan umum terkait pada produksi kentang adalah penggunaan benih sumber yang kurang bermutu dan mengalami kemunduran benih. Penelitian bertujuan untuk menentukan zat penghambat tumbuh coumarin, jasmonic acid dan paklobutrazol yang tepat untuk pertumbuhan stek dan pembentukan ubi mikro kentang secara in vitrosehingga dapat digunakan untuk penyediaan bibit berkualitas. Percobaan dilaksanakan di Laboratorium Kultur Jaringan Teknologi Benih Fakultas Pertanian Universitas Padjadjaran Jatinangor, Sumedang, pada bulan Oktober 2015 sampai Mei 2016. Metode percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) yang merupakan kombinasi dari media, retardan coumarin (105 mg L-1 dan 120 mg L-1), paklobutrazol (0.4 mg L-1 dan 1 mg L-1) …

The Gmo/Ge Debate, Joanna K. Sax

Texas A&M Law Review

We live longer and healthier lives because advances in science create easier and better ways to sustain and survive. Society has an intricate relationship with biotechnology. Vaccines save lives. Fluoridated water decreases dental issues. Antibiotics treat bacterial infections. Nuclear power is a form of clean energy. With any emerging technology, the benefits do not exist in a vacuum, thus, negative consequences result as well. Our widespread uses of antibiotics are creating antibiotic-resistant strains of bacteria. Our research into nuclear energy also facilitated the creation of nuclear bombs. Perhaps it is human nature to use scientific advances for good and for …

Recognizing Challenges And Opportunities In The Quest To End Hunger, Jennifer Williams Zwagerman

Texas A&M Law Review

As an attorney and professor that does not focus on intellectual property law, I was a bit apprehensive about providing a keynote address for a Symposium focusing on “Agriculture, Intellectual Property, and Feeding the World in the 21st Century.” As I thought about this topic, knowing that there were other speakers who would focus more on the IP issues and technical aspects of various topics, I kept coming back to the importance of technology as we worktowards the goal of feeding the world, and the many ways in which innovation plays a role in meeting that goal. It also brought …

Impact Of Burkholderia Phytofirmans Strain Psjn On The Ex Vitro Acclimatization And In Vitro Propagation Of Tissue Cultured Dionaea Muscipula (Venus Fly Trap), Peter L. Riley

Masters Theses, 2010-2019

Dionaea muscipula, Venus fly trap, is an endangered plant that is propagated primarily through tissue culture. Tissue cultured plants must be acclimatized to their new environments when removed from their test tubes and the ensuing stress can result in plant mortality. A plant growth promoting rhizobacterium, Burkholderia phytofirmans strain PsJN, has been utilized in biotic hardening of tissue cultured plants and in the amelioration of stresses, particularly drought stress in potted plants. We investigated whether this bacterium could improve either propagation or acclimatization of tissue cultured Venus fly traps. Standardized inoculations were performed by adjusting cell density of inoculant …

Understanding The Mechanisms Of Insecticide Resistance In Phlebotomus Papatasi And Lutzoymia Longipalpis Sand Flies (Diptera: Psychodidae: Phlebotominae), David Denlinger

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Sand flies, like mosquitoes, ticks, fleas, and lice, transmit pathogens that cause disease in humans. Leishmaniasis, caused by pathogens transmitted by sand flies, kills tens of thousands of people every year. Insecticides have been used to control sand flies, but there is evidence of insecticide resistance in populations of sand flies around the world. The goal of this dissertation was to develop tools to maintain sand flies in the laboratory, develop the ability to identify insecticide-resistant populations of sand flies, and to investigate the genetic mechanisms of how sand flies become resistant to insecticides. I began by comparing live animal …

Towards The Development Of A Sustainable Soya Bean-Based Feedstock For Aquaculture, Hyunwoo Park, Steven Weier, Fareha Razvi, Pamela A. Peña, Neil A. Sims, Jennica Lowell, Cory Hungate, Karma Kissinger, Gavin Key, Paul Fraser, Jonathan Napier, Edgar B. Cahoon, Thomas Clemente

Center for Plant Science Innovation: Faculty Publications

Soya bean (Glycine max (L.) Merr.) is sought after for both its oil and protein components. Genetic approaches to add value to either component are ongoing efforts in soya bean breeding and molecular biology programmes. The former is the primary vegetable oil consumed in the world. Hence, its primary usage is in direct human consumption. As a means to increase its utility in feed applications, thereby expanding the market of soya bean coproducts, we investigated the simultaneous displacement of marine ingredients in aquafeeds with soya bean-based protein and a high Omega-3 fatty acid soya bean oil, enriched with alpha-linolenic …

Transcriptional And Post-Translational Regulation Of Terpenoid Indole Alkaloid Biosynthesis In Catharanthus Roseus, Priyanka Paul

Theses and Dissertations--Plant and Soil Sciences

Catharanthus roseus (Madagascar periwinkle) is the exclusive source of an array of terpenoid indole alkaloids (TIAs) that are used in the treatments of hypertension and certain types of cancer. TIA biosynthesis is under stringent spatiotemporal control and is induced by jasmonate (JA) and fungal elicitors. Tryptamine, derived from the indole branch, and secologanin from the iridoid branch are condensed to form the first TIA, strictosidine. Biosynthesis of TIA is regulated at the transcriptional level and several transcription factors (TFs) regulating the expression of genes encoding key enzymes in the pathway have been isolated and characterized. The JA-responsive APETALA2/ETHYLENE RESPONSE FACTOR …

Neutral And Adaptive Genetic Variation In North American Hardwood Tree Species, Sudhir Khodwekar

Dissertations, Master's Theses and Master's Reports

'Northern Hardwoods,’ are a characteristic composition of multiple tree species and a part of the rich and diverse northeastern forests. Hardwoods e.g. oaks (Quercus: Fagaceae and Sugar maple), serve as foundation species and offer ecological and economical gains to wildlife and humans. Most of the forest trees go through many biotic and abiotic stresses, for example climate change in their long life span. To overcome these threats and to adapt to changing conditions, tree species need to maintain variation especially in adaptive genes. Therefore, it is necessary to analyze genetic diversity within forest tree populations and take appropriate …

Pertumbuhan Eksplan Meristem Bawang Putih (Allium Sativum L.) Kultivar Tawangmangu Pada Berbagai Komposisi Kinetin Dan Ga3 In Vitro, Y. Sholihin, Erni Suminar, Wieny Heriliya Rizky, G. G. Pitaloka

Jurnal Kultivasi

Bawang putih merupakan komoditas penting di Indonesia. Indonesia merupakan negara terbesar pengimpor bawang putih. Permasalahan yang dihadapi dalam teknik produksi benih secara konvensional menjadi salah satu faktor pembatas tidak optimalnya pengembangan dan penyebaran benih tersebut. Produksi benih in vitro dapat dijadikan sebagai metode alternatif dalam perbanyakan benih sumber kultivar unggul. Penelitian ini bertujuanuntuk mendapatkan komposisi kinetin dan GA3 terbaik pada media dasar MS untuk regenerasi eksplan meristemsecara in vitro.Penelitian dilaksanakan di Laboratorium Kultur JaringanBalai Pengembangan Benih Hortikultura dan Aneka Tanaman Pasir Banteng, Kecamatan Jatinangor, Kabupaten Sumedang, Provinsi Jawa Barat, yang berlangsung dari bulan April hingga Juli 2015. Eksplan yang digunakan …

Identification And Characterization Of Genes Involved In Metabolism Of N5 Monoene Precursors To N5 Anacardic Acids In The Trichomes Of Pelargonium X Hortorum., Richa A. Singhal

Electronic Theses and Dissertations

Unusual monoenoic fatty acids (UMFA’s) and specialized metabolites called anacardic acids (AnAc) are produced in glandular trichomes of Pelargonium ´ hortorum (geranium). The UMFA’s, 16:1^∆11 and 18:1^∆13 are precursors for the synthesis of unsaturated AnAc 22:1n5and 24:1n5 that contribute to pest resistance in geraniums. UMFAs and their derived AnAc metabolites not only provide a useful biological marker that differentiates the biosynthetic pathway for unusual mononenes from the common fatty acids (i.e. stearic, palmitic, oleic, linoleic and linolenic) but also have industrial, medical and agricultural applications. Fatty acid biosynthesis enzymes like acyl carrier proteins (ACPs); thioesterases (TEs) and β-ketoacyl-ACP …

Pupuk Anorganik Sebagai Alternatif Media Untuk Pertumbuhan Eksplan Nilam (Pogostemon Cablin Benth) Kultivar Sidikalang Dan Tapaktuan Secara In-Vitro, Suseno Amien, M. F. Wiguna

Jurnal Kultivasi

Kultur jaringan selama ini dipahami oleh sebagian masyarakat merupakan suatu teknologi yang mahal, terutama dalam menyediakan bahan kimia untuk media. Hasil percobaan sebelumnya menunjukkan bahwa percobaan dengan media pupuk anorganik dapat digunakan sebagi media tanam in-vitro. Namun media tersebut belum diteliti secara rinci manfaatnya untuk tanaman nilam. Penelitian ini dilakukan untuk memperoleh media pupuk anorganik yang sesuai untuk pertumbuhan kultivar nilam (Pogostemon cablin Benth) Sidikalang dan Tapak Tuan. Penelitian dilakukan di laboratorium teknologi kultur jaringan Pemuliaan Tanaman Fakultas Pertanian Universitas Padjadjaran dari bulan Mei sampai Agustus 2010. Rancangan yang digunakan adalah rancangan acak lengkap pola faktorial dengan dua faktor perlakuan …

Pengujian Efektivitas Berbagai Jenis Dan Konsentrasi Sitokinin Terhadap Multiplikasi Tunas Mikro Pisang (Musa Paradisiaca L.) Secara In Vitro, Bella Dian Ratnasari., Erni Suminar, Anne Nuraini, Ade Ismail

Jurnal Kultivasi

Perbanyakan tanaman pisang secara konvensional dengan menggunakan bonggol atau anakan (sucker) secara terus menerus dapat menurunkan kualitas dan memerlukan waktu lama serta meningkatkan terjadinya serangan penyakit. Oleh karena itu, suatu upaya untuk menghasilkan bibit yang memiliki kualitas baik dalam waktu yang cepat dapat menggunakan kultur in vitro. Percobaan ini bertujuan untuk memperoleh laju multiplikasi yang tinggi dengan penggunaan salah satu jenis dan konsentrasi sitokinin pada kultur in vitro tanaman pisang kepok kuning. Percobaan dilakukan pada bulan Oktober hingga Desember 2015 di Laboratorium Kultur Jaringan Teknologi Benih Fakultas Pertanian Universitas Padjadjaran. Rancangan percobaan menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari …

Genetic Engineering And Sustainable Crop Disease Management: Opportunities For Case-By-Case Decision-Making, Paul Vincelli

Plant Pathology Faculty Publications

Genetic engineering (GE) offers an expanding array of strategies for enhancing disease resistance of crop plants in sustainable ways, including the potential for reduced pesticide usage. Certain GE applications involve transgenesis, in some cases creating a metabolic pathway novel to the GE crop. In other cases, only cisgenessis is employed. In yet other cases, engineered genetic changes can be so minimal as to be indistinguishable from natural mutations. Thus, GE crops vary substantially and should be evaluated for risks, benefits, and social considerations on a case-by-case basis. Deployment of GE traits should be with an eye towards long-term sustainability; several …

Lipid Content And Biomass Analysis In Autotrophic And Heterotrophic Algal Species, Addie M. Lauder, Daniel P. Jones, Thomas E. Walker, Todd Allen

Montview Journal of Research & Scholarship

Biofuels are a form of renewable energy derived from living matter, typically plants. The push for biofuels began in order to decrease the amount of carbon dioxide (CO2) released into the atmosphere, as biofuels are essentially carbon neutral. The idea is the same amount of CO2 the plants took in to perform photosynthesis will then be released in the burning of the biofuels. Algae is an excellent source of biofuels because it grows quickly and is versatile in terms of the type of fuel it can produce. The two most common mechanisms for algae growth are heterotrophic or photoautotrophic. Heterotrophically …

Effects Of Soil Type And Diversity On Soil Respiration And Litter Decomposition In A Perennial Biofuel Production System, Jordan Young

Annual Graduate Student Symposium

For my master's thesis, I researched soil respiration and litter decomposition at the Cedar River Ecological Research Site. Respiration and decomposition are important processes in the understanding of carbon sequestration, which is one of the main benefits of using biofuels. Cellulosic biofuels can be produced from a wide diversity of plants on a wide range of soil types, and these factors can influence the rate of carbon sequestration, so I investigated the effects of plant diversity and soil type in the context of perennial biofuel production.

Plantbot Genetics Presents: The Moth Project, Augustana College, Rock Island Illinois

2015-2016

PlantBot Genetics Inc. presents an engaging overview of second shift pollinators such as the moth, along with recent advances in self-pollinating robo-plants. The Moth Project highlights inspired responses to the decline of the honeybee and the hope that Moths and PlantBots will step in and save the day.

As consumers, we no longer know the real price of our food. Present food distribution systems are so complicated that it is unrealistic to expect consumers will make responsible choices based on the knowledge at hand. We encourage people to think more about their food, where it comes from, and where it …

Bio-Piracy: Creating Proprietary Rights In Plant Genetic Resources, James O. Odek

Journal of Intellectual Property Law

No abstract provided.

Drought Responsive Gene Expression Regulatory Divergence Between Upland And Lowland Ecotypes Of A Perennial C4 Grass, John T. Lovell, Scott Schwartz, David B. Lowry, Eugene V. Shakirov, Jason E. Bonnette, Xiaoyu Weng, Mei Wang, Jenifer Johnson, Avinash Sreedasyam, Christopher Plott, Jerry Jenkins, Jeremy Schmutz, Thomas E. Juenger

Biological Sciences Faculty Research

Climatic adaptation is an example of a genotype-by-environment interaction (G×E) of fitness. Selection upon gene expression regulatory variation can contribute to adaptive phenotypic diversity; however, surprisingly few studies have examined how genome-wide patterns of gene expression G×E are manifested in response to environmental stress and other selective agents that cause climatic adaptation. Here, we characterize drought-responsive expression divergence between upland (drought-adapted) and lowland (mesic) ecotypes of the perennial C₄ grass, Panicum hallii, in natural field conditions. Overall, we find that cis-regulatory elements contributed to gene expression divergence across 47% of genes, 7.2% of which exhibit drought-responsive G×E. …

Biological Lignocellulose Solubilization: Comparative Evaluation Of Biocatalysts And Enhancement Via Cotreatment, Julie M. D. Paye, Anna Guseva, Sarah K. Hammer, Erica Gjersing

Dartmouth Scholarship

Feedstock recalcitrance is the most important barrier impeding cost-effective production of cellulosic biofuels. Pioneer commercial cellulosic ethanol facilities employ thermochemical pretreatment and addition of fungal cellulase, reflecting the main research emphasis in the field. However, it has been suggested that it may be possible to process cellulosic biomass without thermochemical pretreatment using thermophilic, cellulolytic bacteria. To further explore this idea, we examine the ability of various biocatalysts to solubilize autoclaved but otherwise unpretreated cellulosic biomass under controlled but not industrial conditions.