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Full-Text Articles in Biotechnology
Pollen-Specific Expression Of Ecori Restriction Endonuclease For Bioconfinement In Panicum Virgatum L., Jessica Nicole Stockdale
Pollen-Specific Expression Of Ecori Restriction Endonuclease For Bioconfinement In Panicum Virgatum L., Jessica Nicole Stockdale
Masters Theses
The dispersal of pollen from genetically engineered (GE) crops can lead to unintended transgene flow, especially in wind-pollinated and obligate-outcrossing species such as switchgrass (Panicum virgatum L.). Various bioconfinement approaches have been studied for their potential to eliminate transgene flow from GE crops including selective male sterility, which aims to inhibit the germination of transgenic pollen grains. In this study, pollen-specific promoters controlling the expression of the EcoRI endonuclease, interrupted by a catalase intron, was evaluated for its efficacy to produce sterile pollen. The TaPSG719, PvPS1, Osg6B, OsRTS, and Zm13 promoters were assessed for pollen-specific expression patterns, none …
Bioconfinement Of A Putatively Sterile Nicotiana Hybrid And Development Of Tools For Assessing Gene Flow, John Hollis Rice
Bioconfinement Of A Putatively Sterile Nicotiana Hybrid And Development Of Tools For Assessing Gene Flow, John Hollis Rice
Masters Theses
Production of transgenic crops in open field environments is an ongoing concern of due to the potential for gene flow. New transgenic crops, such as plant-made-pharmaceuticals may generate additional concerns about effects of adventitious transgenes. Use of a bioconfinement strategy may alleviate any consequences by preventing gene flow. The following chapters discuss previous and current research on gene flow, testing of a Nicotiana hybrid system for bioconfinement efficiency, and development of methods for transgene detection. The candidate ‘platform plant’ that was tested is a Nicotiana hybrid (Nicotiana tabacum ‘TN 90’ × Nicotiana glauca) previously identified to be sexually …
Consequences Of Gene Flow And Transgene Introgression In Hybrids Between Transgenic Brassica Napus And Its Weedy Wild Relative Brassica Rapa, Reginald Jason Millwood
Consequences Of Gene Flow And Transgene Introgression In Hybrids Between Transgenic Brassica Napus And Its Weedy Wild Relative Brassica Rapa, Reginald Jason Millwood
Masters Theses
The adventitious presence of transgenes and their potential impact on the environment has been a topic of concern for many years. To address these concerns the following chapters discuss past and current research of gene flow and introgression, methods for transgene detection and monitoring, and the results from field-level experiments using artificially introgressed advanced generation hybrids. The field studies were designed to be a worst-case scenario where hybrids were produced by hand-crossing transgenic Brassica napus (AACC, 2n = 38) and its weedy wild relative Brassica rapa (AA, 2n = 20). B. napus was transgenic for the green fluorescent protein [m-GFP-5 …
If Hybrids Go Wrong: Assessing Potential Environmental Risk From Release Of Herbicide-Resistant Creeping Bentgrass (Agrostis)., Carol Auer, Collin Ahens
If Hybrids Go Wrong: Assessing Potential Environmental Risk From Release Of Herbicide-Resistant Creeping Bentgrass (Agrostis)., Carol Auer, Collin Ahens
Plant Science Presentations and Proceedings
Genetically-modified (GM) plants need to be assessed for their benefits and risks before they are released into the environment. At present, GM grasses are being developed to improve sports turf and biofuels crops (e.g. switchgrass). How will genetic engineering of perennial grasses alter the distribution of these grasses in natural areas and managed landscapes? Can we use research data to predict future gene flow, weediness, or invasion? These are some of the important questions in ecological risk assessment of GM perennial grasses. We have initiated five research projects to characterize gene flow and potential ecological risk from herbicide-resistant (HR) creeping …
Integration Of Gis And Bentgrass Ecology For Ecological Risk Assessment., Carol Auer, Collin Ahrens
Integration Of Gis And Bentgrass Ecology For Ecological Risk Assessment., Carol Auer, Collin Ahrens
Plant Science Presentations and Proceedings
All new technologies carry both benefits and risks. Genetically-modified plants must be assessed before they are released into the environment. Our research examines plant gene flow and the potential impacts from environmental release of genetically-modified plants. Plant gene flow is a natural process that occurs when pollen from one plant lands on the flower of another plant and produces a hybrid offspring. Gene flow can produce hybrid offspring with new traits that could change the ability of the plant to survive and spread. If hybrid offspring have some advantage in the environment, they could become invasive and/or affect other components …
When Hybrids Go Wrong. How Hybridization Can Create Invasive Species., Carol Auer, Collin Ahrens
When Hybrids Go Wrong. How Hybridization Can Create Invasive Species., Carol Auer, Collin Ahrens
Plant Science Presentations and Proceedings
Gene flow is the movement of genes from one plant population to another. Gene flow is a natural process and a part of plant evolution. There are two ways for gene flow to occur in plants. The first is through sexual reproduction – pollen lands on a flower and a viable seed develops. The second method is through dispersal of seeds and/or vegetative plant parts (e.g. stolons, rhizomes). Gene flow can produce hybrid offspring with an increased or decreased ability to survive in the landscape. If hybrid offspring have some advantage in the environment, they could become invasive. This poster …