Biotechnology Commons^™

Snake Optimization Algorithm For Fractional Order Controller In Three-Area Load Frequency Regulation, Shubra Goel, Omveer Singh

ASEAN Journal on Science and Technology for Development

The transition toward renewable energy-dominated power systems have accentuated the intricacies of frequency control, necessitating advanced regulatory mechanisms. This investigation articulates a tri-zonal frequency stabilization approach, employing a hybridized controller that synergizes Fuzzy Fractional-Order PI and Tilt-Integral-Derivative methodologies. The uniqueness of this approach is further amplified by the deployment of the Snake Optimization algorithm for precise parameter tuning. Set within a conventional grid topology integrated with assorted renewable energy sources, the study evaluates the controller’s adaptability and resilience through a series of comprehensive scenario-based analyses.

Quality And Safety Analysis Of Integrated Bus Services And Feeder Transport In Surabaya, Indonesia, Desrina Yusi Irawati, Agrienta Bellanov, Armadeo Ruben Canariesa

ASEAN Journal on Science and Technology for Development

The Surabaya city government has developed public transportation through integrated bus services and feeder transport. These transportation systems include Trans Semanggi, Suroboyo Bus, and Wira Wiri Surabaya. The challenge facing these transportation programs is the low level of public participation in using public transportation. This research aims to provide a comprehensive investigation of the perceived service quality of integrated public transportation in Surabaya by combining the RESCA and SERVQUAL frameworks. The study contributes by highlighting service quality gaps across five customized dimensions and offering practical insights to improve user satisfaction and increase public transport usage. Each RESCA dimension incorporates RATER …

The Effect Of Blanching And Steaming Pretreatment On The Quality Of Durian (Durio Zibenthinus Murr.) Seed Flour, Nabilah Daniel, Rosnah Shamsudin, Mohd Sabri Pak Dek, Jhauharotul Muchlisyiyah, Masmunira Rambli

ASEAN Journal on Science and Technology for Development

Durian (Durio zibethinus Murr.), the "King of Fruits," has seeds that offer nutritional benefits, including high fiber for gut health and high protein for muscle growth. However, natural browning enzymes can limit its use in baking. This study investigates the comparative effects of blanching and steaming durations (5, 10, 15, 20, and 25 minutes) on the color and techno-functional properties of durian seed flour (DSF), providing an optimization-based approach rarely reported for this material.. Durian seeds were sourced from various stalls in Malaysia, washed, soaked in 0.6% sodium metabisulfite, and then blanched or steamed for for varying durations as …

Seismic Vulnerability Assessment With Rapid Visual Screening Of Hospital Buildings In Maguindanao Province, Philippines, Ebrahim B. Kayog, Kristine S. Companion, Kenny B. Cantila, Elizabeth Edan M. Albiento

ASEAN Journal on Science and Technology for Development

Hospitals are expected to maintain operations during and after earthquakes, yet many facilities in the Philippines remain structurally and non-structurally vulnerable. This study evaluates the seismic vulnerability of selected public hospitals in Maguindanao Province, located near the Cotabato Trench and the Western Mindanao Fault, using the FEMA P-154 (3rd ed.) Rapid Visual Screening (RVS) Level-1 procedure. Four government hospitals, comprising 53 buildings constructed between 1980 and 2025, were assessed through a systematic visual inspection and review of available secondary records. Basic structural hazard scores were adjusted using FEMA score modifiers (performance modification factors) to compute Final Level-1 scores (Sₗ₁) and …

Assessing The Probable Sources Affecting The Water Quality Index Of The Panch Prayag Belt Of Uttarakhand, India, Debasis Sau, Indranil Mukherjee, Wasim Akram, Kakali Ghosh, Rimi Paul, Reshmi Paul, Athar Akram

ASEAN Journal on Science and Technology for Development

The Water Quality Index (WQI) is an essential metric for evaluating the usability of surface water resources, particularly in ecologically sensitive and high-demand areas like the Panch Prayag belt of Uttarakhand, India. This region, comprising five major pilgrimage towns—Devaprayag, Nandprayag, Vishnuprayag, Karnaprayag, and Rudraprayag—faces seasonal fluctuations in water quality due to both natural and anthropogenic pressures. In this study, water samples were collected from 2021 to 2023 across pre-monsoon, monsoon, and post-monsoon seasons, and the WQI was computed using the Canadian Water Quality Index (CWQI 1.0). Results revealed that WQI values ranged from 36 to 45 across locations and seasons, …

Manure Handling In Circular Farming: An In Vitro Study Of Fermented Poultry Manure As Unconventional Feed For Ruminants, Dimas Hand Vidya Paradhipta, De Lavida Padma Terrakota, Ali Agus, Andriyani Astuti, Viagian Pastawan, Kharisma Taufiqa Hidayah

ASEAN Journal on Science and Technology for Development

The development of the poultry industry must consider the principle of circular economy. Therefore, this study aims to develop a method of fermenting poultry manure as a new, unconventional feed, which can support the model of circular farming. Poultry manure obtained from laying hens was mixed with cassava solid waste at a ratio of 7:3 to maintain proper moisture content. The mixed manure fermented for 14 days into a 30 kg silo in triplicate using different additives, namely Lactiplantibacillus plantarum FNCC 0020 at 1 x 10⁵ cfu/g (LP), Bacillus cereus LS2B as proteolytic bacteria at 1 x …

Numerical Modeling Of Performance And Phenomena In A Single Proton Exchange Membrane Fuel Cell, Doan Nguyen Cong, Hiep Nguyen Ha

ASEAN Journal on Science and Technology for Development

One of the methods to protect our environment is to convert the fuel's chemical energy directly into electricity, bypassing the fuel's combustion process. This method is implemented in devices called fuel cells. Among them, the proton exchange membrane fuel cell (PEMFC) is widely used in practice because it has outstanding advantages such as high power density and efficiency, low operating temperature and noise, compactness, fast start-up, and, most importantly, only releases water, entirely safe for the environment. The paper presents a method for selecting materials for manufacturing PEMFC components, making a single PEMFC model based on the materials chosen, then …

Enhancing The Mechanical Properties Of Sla 3d-Printed Bio-Resin From Linseed Oil Using Cellulose And Lignin Biopolymers, Chukwunonso A. Ikedionu

College of Graduate Studies: Theses & Dissertations

Abstract

This research explores the development of bio resin from linseed oil by the process of epoxidation and acrylation to produce acrylated epoxidized linseed oil (AELO). Lignin and cellulose biopolymers were added as reinforcement at varying weight fractions of 1 wt.%, 2.5 wt.%, and 5 wt.%, and their mechanical performance was compared against pure AELO. The formulations were printed using an Elegoo SLA 3D printer to create standardized test specimens like tensile bars, compression cylinders, and hardness blocks, which were all prepared according to the ASTM testing standard.

The results indicate that reinforcement effects depend on both filler type and …

Black Soldier Fly Larvae (Bsfl) Bioconversion For Circular Economy: A Study In Polaman Village, Siswo Sumardiono, Rizka Amalia, Syaikha Butsaina Dhiya’Ulhaq, Nurika Nazilatul Ilmi, Hermawan Dwi Ariyanto

ASEAN Journal of Community Engagement

Conventional organic waste management methods often lead to environmental degradation and underutilization of valuable resources. In rural areas, such as Polaman Village, limited awareness and inefficient disposal systems exacerbate these issues, thus necessitating innovative and sustainable solutions. This study explores black soldier fly larvae (BSFL) bioconversion as a community-based solution for organic waste management, addresses environmental challenges, and promotes economic empowerment by converting waste into valuable by-products. The study employed a mixed-methods approach, beginning with baseline assessments of waste generation and existing management practices to identify community needs. Educational workshops and hands-on training sessions introduced BSFL bioconversion techniques to local …

Determination Of The Kinetic Parameters Of Cholesterol Oxidation Using Cholesterol Oxidase From Streptomyces Sp., Meka Saima Perdani, Heri Hermansyah, Muhamad Sahlan, Dwini Normayulisa Putri, Teguh Pambudi, Anggi Khairina Hanum Hasibuan

Makara Journal of Technology

Cholesterol oxidase (CO) was successfully produced from Streptomyces sp. via the submerged fermentation method, and 69 U/mL enzyme activity was obtained. This study aimed to determine cholesterol oxidation kinetics and the production of CO as a catalyst. The enzyme was diluted to 0.15, 0.075, and 0.00375 U/mL for the oxidation reaction. The substrate was also prepared in three concentrations: 3.23, 6.46, and 12.93 mM. The optimization of conditions for enzymatic cholesterol oxidation was investigated through measurement of the effect of initial cholesterol and enzyme concentrations. Cholesterol concentration was rapidly measured via high-performance liquid chromatography (HPLC). The kinetics of CO were …

Identification Of Conserved Eukaryotic Gene Products In Novel Mycobacteriophage, Brandon Mar, Jade Coxon, Lily Esterline, Sydney Clifford, Gabriella Burke, Mallory Motz, Alaina Gartner, Kioni Bush, James Lin, Lizzie Adams, Sophia Palant

The Journal of Purdue Undergraduate Research

Bacteriophage, a type of virus that infects bacteria, was discovered 100 years ago, initially isolated with a plaque assay in the 1940s. A mycobacteriophage is a subtype of bacteriophage that specifically infects mycobacteria. Since the introduction of DNA technologies and efforts to identify a plethora of bacteriophages, over 10,000 bacteriophages have been identified with 1,500 sequenced. Bacteriophage discovery has aided in genetic editing techniques, the development of synthetic biology tools, and disease treatment. However, to be able to expand on these capabilities, there is a need to identify more bacteriophages and sequence the genomes of these viruses, as they outnumber …

Analysing Sound Characteristics Of Cello And Violin Using Fast Fourier Transform, Sinin Hamdan, Khairul Anwar Mohamad Said, Ahmad Faudzi Musib, Marini Sawawi, Aaliyawani Ezzerin Sinin

ASEAN Journal on Science and Technology for Development

The unique sound characteristics of music are based on multiple harmonic frequencies that exist within the sound waves. Through Fast Fourier Transform (FFT) software, the wave can be broken down into frequency and amplitude components. Spectrum analysis can be used quantitatively to describe these sound characteristics. In this paper, the frequency range present in the spectrum and the average intensity of the first 10 high notes in the sound are used to classify the sound characteristics of the cello and violin. This is done by generating a frequency (x-axis) and amplitude (y-axis) graph for the sounds of the cello and …

Use Of Electrical Bone Growth Stimulators In High-Risk Patients Following Spinal Fusion, Soumya Malhotra, Khavir Sharieff

HCA-NSU MD Research Day

Title: Use of Electrical Bone Growth Stimulators in High-Risk Patients Following Spinal Fusion Authors: Soumya Malhotra, BA., MS1 & Khavir Sharieff, DO, MBA2 1Class of 2027, Nova Southeastern University Dr. Kiran C. Patel College of Osteopathic Medicine, Fort Lauderdale, FL 2Assistant Professor of Surgery, Nova Southeastern University Dr. Kiran C. Patel College of Osteopathic Medicine, Tampa Bay, FLNova Southeastern University Dr. Kiran C. Patel College of Osteopathic Medicine Objective: This study aimed to elucidate the advantages of EBGSs following high-risk spinal fusions. Background: Pseudarthrosis is a condition where bones fail to fuse after an injury or surgery. It causes significant …

The Effects Of Wildfires On The Mental And Physical Health Of School-Age Children In North America: A Scoping Review, Sarah Oerther, Sarah Manspeaker, Alison Wix, Daniel Oerther, Carmen Marsit

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

Background: Wildfires are occurring more often and with greater intensity, leading to longer and harsher fire seasons. As a result, children are more frequently exposed to wildfire smoke, which increases their risk of mental and physical health effects. Purpose: The purpose of our scoping review is to explore the current literature on what is known about the direct and indirect impacts of wildfires on the mental and physical health of school-age children in North America (5 to 18-year-olds). Methods: A health sciences librarian-assisted literature search, with input from the research team, was performed. Results: A total of 16 studies were …

An Experiment Study On Implementing Syngas On A Small Generator Set, Bui Van Chinh, Khong Vu Quang, Khanh Nguyen Duc

ASEAN Journal on Science and Technology for Development

This paper presents an experiment on syngas's influence on the performance and pollutant characteristics of a mechanical diesel engine generator set. Using syngas as an external fuel is good for medium and light loads of less than 80%. While a moderate substitution of syngas will contribute to good performance at high loads, a larger level of syngas provided may degrade engine performance. The brake-specific energy consumption rises as the replacement syngas flow increases. Using syngas as an external fuel to substitute for a part of the diesel might cause the engine to perform unstablely. The more syngas replacements, the greater …

Advances In Biomedical Research And Treatments: What Is Acceptable?, Michael L. Clancy, Khalid M. Iskandarani, Mitchell C. Mccrea

Journal of Health Ethics

This study investigated the technology acceptance (TA) of twenty-first century biomedical treatments by adults in the United States. A new TA instrument was created, using five distinct levels: (1) Healing and Prevention, (2) Replacement Organs, (3) Enhancements-Medical, (4) Enhancements-Discretionary, and (5) Transhumans. An on-line survey produced 353 usable responses, which showed distinct patterns for each of five biomedical treatment levels. There was clear support for Levels 1–3, but very strong opposition to Levels 4–5. The TA finding draws the line between which human interventions are acceptable versus others that should be prohibited through public policies and medical guidelines.

A P-Graph Approach For Planning Sustainable Rice Straw Management Networks, Victoria Migo Sumagang Maria, Michael Angelo B. Promentilla

The Philippine Agricultural Scientist

The Philippines produces up to 15.2 Mt of rice straw waste annually. Unmanaged rice straw waste disposal can lead to pollution from open field burning. On the other hand, rice straw agricultural waste can be used sustainably to produce valuable products such as mushrooms, fodder, pellets, or bioenergy, in a rice straw management system. Such systems can be optimized using Process Integration tools so that the raw materials are used efficiently at the maximum profit and with a minimal carbon footprint. The P-graph framework is an efficient Process Integration tool that solves Process Network Synthesis problems. A P-graph finds the …

Epidural Stimulation For Urogenital Dysfunction In The Spinal Cord Injured Rat., Natasha Lorraine Wilkins

Electronic Theses and Dissertations

Spinal cord injury (SCI) is a devastating, life changing event that greatly impacts quality of life. SCI can have body-wide, multi-system consequences including dysfunction within the urogenital system. Challenges with the urinary system include urinary incontinence, urine retention, and detrusor-sphincter dyssynergia all of which require maintenance through the regular use of catheters. Sexual dysfunction in men, who comprise nearly 80% of the SCI population, includes erectile dysfunction, anejaculation, retrograde ejaculation, poor expulsive force during ejaculation, and poor sperm quality. The treatment for sexual function in SCI men is focused more on fertility through the collection of sperm for insemination procedures …

Dynamic Metabolic Flux Analysis Amidst Data Variability: Sphingolipid Biosynthesis Case Study In Arabidopsis Thaliana Cell Cultures, Abraham Boluwatife Osinuga

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

Sphingolipids are pivotal for plant development and stress responses. Growing interest has been directed towards fully comprehending the regulatory mechanisms of the sphingolipid pathway. In this study, we explore its de novo biosynthesis and homeostasis in Arabidopsis thaliana cell cultures, shedding light on fundamental metabolic mechanisms. Employing ¹⁵N isotope labeling and quantitative dynamic modeling approach, we obtained data with notable variations and developed a regularized and constraint-based Dynamic Metabolic Flux Analysis (r-DMFA) framework to predict metabolic shifts due to enzymatic changes. Our analysis revealed key enzymes such as sphingoid-base hydroxylase (SBH) and long-chain-base kinase …

Optimizing The Expression Of Polyethylene Terephtalate Hydrolase-Encoding Synthetic Gene In Escherichia Coli Arctic Express (De3), Jocelyn Nataniel, Maria Ulfah, Dini Achnafani, Niknik Nurhayati, Gabriela Christy Sabbathini, Sri Rezeki Wulandari, Abinawanto Abinawanto, Is Helianti

Makara Journal of Science

The waste of polyethylene terephthalate (PET) plastic waste in Indonesia is a pressing concern due to its slow degradation and potential environmental damage. One promising solution is to utilize polyethylene terephthalate hydrolase from Ideonella sakaiensis (IsPETase), an enzyme that specifically degrades PET. However, inducing the expression of IsPETase synthetic gene in Escherichia coli BL21 (DE3) has been challenging because much of it remains insoluble. This study aimed to express IsPETase in E. coli Arctic Express (DE3) and optimize the conditions to enhance its production. First, pET22b(+)pelB-IsPETase was inserted into E. coli Arctic Express (DE3). The …

Naturify 2300, Yarina Yiwei Dai

Masters Theses

In my art practice, I explore the interplay between human desires to manipulate and anthropomorphize nature, as seen in the technological augmentation of plants and living entities. This investigation delves into how this intersection, alongside empathy towards these creations, contributes to fears of uncontrollability and the risks of addiction and excessive dependence on technology.

Bioengineering and genetic modification have cultivated unprecedented developments, allowing humans to manipulate the fundamental building blocks of life. My research speculates on this technology further, modifying the genetic code of organisms and creating bioengineered wearable entities with enhanced traits or entirely new functionalities. The primary objective …

Transcriptomic Profiling Of Engineered Human Gene Integration In The Mouse Genome Following In Vitro Gene Editing, Ethan Potts, Made Harumi Padmaswari, Christopher Nelson

Biomedical Engineering Undergraduate Honors Theses

Gene replacement is a promising method of therapy for genetic diseases. However, safety and efficacy are areas that need more research. This experiment aims to use RNA sequencing and bioinformatic techniques to provide answers to these questions and provide direction to future studies to develop a gene replacement therapeutic. C2C12 mouse myoblast cells were transfected with a vector containing a CRISPR-Cas9 system and the Human Factor IX (hF9) gene in order to hijack target genes and integrate the hF9 gene. The two target genes, myoglobin (Mb) and creatine kinase (Ckm), were chosen for their high rate of expression and low …

Determining The Impacts Of Corn Silage Mixing Ratios On Hydrogen Production From Beef Cattle Manure, Katya Faber-Quimby

Honors Program: Senior Projects (Public)

With global warming becoming an increasingly serious threat and a growing population requiring additional energy resources, interest in the production of clean energy from the agricultural sector has surged in popularity. Biohydrogen, also known as green hydrogen, is H₂ that has been produced using renewable energy, such as from agricultural byproducts or waste materials. It presents a promising alternative to nonrenewable resources due to its high potential for energy storage and lack of carbon emissions when used. Cattle manure has been explored as a resource for the production of biohydrogen. Selecting for hydrogen-producing microbes in the manure serves the …

Broadening The Application Range Of Cell-Free Protein Expression Systems, Matthew Becker

All Theses

Centralized protein manufacturing platforms make the delivery of needed therapies to places with limited infrastructure almost impossible. Cell-free protein expression systems, systems that utilize protein production machinery extracted from cells, offer these communities a viable protein expression platform that is robust and easily deliverable to the place of need. Much work in cell-free system engineering looks to increase the hardiness of cell-free system (CFS) components, like the extract and reaction buffer, needed to carry transcription and translation of gene therapeutic targets forward. Freeze- and air-drying of extract, reaction buffer, or both with certain additives, like sugar molecules, has been proposed …

Locally Injectable Peptide Hydrogel For Conversion Of Temozolomide And Codelivery Of Sirna In Glioblastoma Multiforme Treatment, Megan Pitz

All Dissertations

Traditional treatment methods for glioblastoma multiforme (GBM) are largely unsuccessful, with a 5-year survival rate of 5.6%. Temozolomide (TMZ), the only chemotherapy FDA-approved for GBM treatment, is a hydrophobic prodrug that is stable in an acidic pH and begins converting to its active form at a physiological pH of 7.4. However, complete conversion of TMZ is observed only at a higher pH, and only about 30% of administered TMZ reaches the central nervous system. Therefore, there is a need for new treatment methods that maximize TMZ delivery to and efficacy at the tumor site. Self-assembling peptides are a unique class …

A Machine Learning Model Of Perturb-Seq Data For Use In Space Flight Gene Expression Profile Analysis, Liam F. Johnson, James Casaletto, Lauren Sanders, Sylvain Costes

Graduate Industrial Research Symposium

The genetic perturbations caused by spaceflight on biological systems tend to have a system-wide effect which is often difficult to deconvolute it into individual signals with specific points of origin. Single cell multi-omic data can provide a profile of the perturbational effects, but does not necessarily indicate the initial point of interference within the network. The objective of this project is to take advantage of large scale and genome-wide perturbational datasets by using them to train a tuned machine learning model that is capable of predicting the effects of unseen perturbations in new data. Perturb-Seq datasets are large libraries of …

Us Public Opinion About The Safety Of Gene Editing In The Agriculture And Medical Fields And The Amount Of Evidence Needed To Improve Opinions, Brandon R. Mcfadden, Joy N. Rumble, Kathryn A. Stofer, Kevin M. Folta

Agricultural Economics and Agribusiness Faculty Publications and Presentations

Introduction: Implementation of gene editing in agriculture and medicine hinges on public acceptance. The objectives of this study were to explore U.S. public opinion about gene editing in agricultural and medical fields and to provide more insight into the relationship between opinions about the safety of gene editing and the potential impact of evidence to improve opinions about safety.

Methods: Data were from two samples of U.S. respondents: 1,442 respondents in 2021 and 3,125 respondents in 2022. Survey respondents provided their opinions about the safety of gene editing in the agricultural and medical fields and answered questions about the number …

On-Site Estimation Of Total Sugars In Flavored Milk Using A Glucometer, Erwin Oliver V. Fundador, Karyn Thea E. Rivera, Kriza Faye A. Calumba, Noreen Grace V. Fundador

ASEAN Journal on Science and Technology for Development

Sugar has been linked to obesity and chronic diseases. Unfortunately some "healthy products," such as flavored milk, contain high sugar. In the Philippines, such an issue is made worse by local brands not putting nutritional labels on their products. Thus, a cheap, home-based method to measure total sugars should be beneficial. In this paper, we have shown that after incubation with invertase and lactase, a glucometer can be used to measure total sugars in flavored milk on-site. All experiments except the weighing of invertase and lactose were done in a home setting for actual proof of concept. The total sugar …

Molecular Identification To Verify The Existence Of Helicotylenchus Dihystera In Coffee Plants At East Java, Indonesia, Ankardiansyah Pandu Pradana, Toyoshi Yoshiga

ASEAN Journal on Science and Technology for Development

Spiral nematodes Helicotylenchus sp. are known to infect a variety of plants, including coffee. This is in line with the studies and surveys of nematodes in Indonesia. However, to date, there has been no study reporting the nematode with molecular analysis. In this study, we surveyed the nematodes in Arabica coffee plantations in Bondowoso regency – Indonesia. These spiral nematodes were collected in DESS solution for morphological, morphometric and molecular analysis. The morphometric analysis was carried out under an Olympus BH-2 compound microscope with an attached calibrated Toupcam M20 camera. The molecular analysis was performed on the total DNA from …

Species Distribution Modelling Using Bioclimatic Variables On Critically Endangered Endemic Species (Macrocephalon Maleo) In Sulawesi, Septianto Aldiansyah, Khalil Abdul Wahid

ASEAN Journal on Science and Technology for Development

The island of Sulawesi isolates migratory fauna from Asia and Australia and creates animal combinations such as the Maleo (Macrocephalon Maleo). The Maleo population is experiencing a downward trend due to habitat fragmentation, deforestation and threats by humans. This study aims to model the potential distribution of Maleo using several famous current and future models as a result of climate change throughout the island of Sulawesi, not only in its natural habitat, but beyond its natural habitat. Bioclimatic variables and in situ attendance data were used in this study. The method used is Maximum Entropy by evaluating the GLM, SVM …