Integrative Biology Commons^™

Academic Burnout In Human Anatomy & Physiology I Students, Shivani Shukla

Honors Theses

Human Anatomy and Physiology I is the study of the function and structure of the human body. It is a challenging class that many students have to take for their undergraduate degree. Due to the vast information that is given in this complex class, students can struggle with understanding the material and knowing how to study thus leading to burnout in the course. Understanding why students undergo burnout in their courses could uncover ways that professors and institutions could help the students to prevent burnout. The goal of this study was to determine the level of burnout in Human Anatomy …

The Prize Biter Of Modernity? Investigating Killer Whale Bite Mechanics, Daniel Hong

West Chester University Graduate Theses, Dissertations, and Final Projects

Although the killer whale (Orcinus orca) is a cosmopolitan apex predator, very little work has been done on its feeding biomechanics. Here I use finite element analysis to investigate the mandibular stiffness of a juvenile male Bigg’s killer whale, and compare it to that of the false killer whale (Pseudorca crassidens) and the saltwater crocodile (Crocodylus porosus). Full mandibles and left and right halves of mandibles are simulated under FEA. I also estimate the total jaw muscle force produced by the Bigg’s killer whale using the dry skull method. The killer whale lacks an …

Comparing Four Indices Of Physiological Stress In An Urban-Adapted Songbird Between Urban And Rural Habitats, Rachel Kaplan

Master of Science in Integrative Biology Theses

Birds show tremendous variation in the extent to which different species tolerate life in urban habitats, yet it remains unclear why some species thrive while others cannot. My work explores possible phenotypes that make an urban-adapted species, the European starling (Sturnus vulgaris), so successful in both rural and urban areas. Literature on stress-urbanization relationship remains highly equivocal partly due to an array of different study systems being compared as well as to key methodological differences. The present study quantified metrics of both acute stress (i.e., stress-induced corticosterone and breath rate) and chronic stress (i.e., baseline corticosterone and total …

Patterns Of Evolutionary Conservation And Divergence In The Short-Term Hyposalinity Stress Response Of A Euryhaline Diatom, Skeletonema Marinoi, Kathryn Judy

Graduate Theses and Dissertations

Survival under fluctuating environmental conditions, such as those increasing in frequency and magnitude under environmental change, requires a successful response to stress. Interspecific differences in stress responses may result in differential survival of species, even within a lineage. Diatoms may constitute one such lineage, as salinity tolerance among extant species is diverse, and the observation of frequent historic habitat transitions between marine and freshwater environments indicates that diatoms successfully mitigated (low) salinity stress in the past, followed by adaptation and diversification over evolutionary time scales. To understand to what extent the diatom hypoosmotic stress response consists of conserved and variable …

The Neuroinflammatory Effects Of Chronic Unpredictable Stress On Zebrafish, Danio Rerio, Brandon Stewart

Master of Science in Integrative Biology Theses

Stress is a state of threatened homeostasis counteracted by various physiologic and behavioral responses aimed to maintain or restore balance. As such, stress acts as a motivator to perform during the challenges of life to survive. Chronic perturbations to the stress response homeostasis without relief can lead to dysregulation, thus attenuating organ systems and structures and causing significant damage ^{1]. Individuals who undergo psychological trauma endure an acute and transient experience, which results in minimal functional impairment, but some suffer from a chronic condition called posttraumatic stress disorder (PTSD). Individuals who have PTSD are likely to experience intense stress, …

Analyzing Physiological Stress Response Using Dermal Swabs In Plethodon Montanus, John Tester

Undergraduate Honors Theses

Upon exposure to environmental stressors, amphibians such as Plethodon montanus will release corticosterone (CORT) thus causing a behavioral and physiological response to cope with the stress. Currently, there are several invasive ways of collecting CORT in salamanders. However, these techniques typically require euthanasia of the organism. We hypothesized that exposure of P. montanus to stressful handling conditions will result in elevations of CORT that can be detected through dermal swabbing. To test this, two experiments were conducted which involved swabbing the dorsal side of the trunk before and immediately after exposing P. montanus to two different environmental stressors. The first …

Stress And Body Composition Of Juvenile Alligator Snapping Turtles (Macrochelys Temminckii), Brandon Scott Tappmeyer

Graduate Theses/Dissertations

The alligator snapping turtle (Macrochelys temminckii), is a species of conservation concern that is the subject of multiple head-start and reintroduction efforts across its range. In captive propagation programs, producing offspring that are in optimal physiological condition maximizes the likelihood of success after release. The purpose of my study was to compare stress and body composition between one free-ranging reintroduced population and two captive populations. The two captive populations were both housed in southern Oklahoma, but one group was reared indoors whereas the other inhabited outdoor ponds at a national fish hatchery. I used circulating glucocorticoid (corticosterone) concentrations as an …

Do Host‐Associated Gut Microbiota Mediate The Effect Of An Herbicide On Disease Risk In Frogs?, Sarah A. Knutie, Caitlin R. Gabor, Kevin D. Kohl, Jason R. Rohr

Integrative Biology Faculty and Staff Publications

1. Environmental stressors, such as pollutants, can increase disease risk in wildlife. For example, the herbicide atrazine affects host defences (e.g. resistance and tolerance) of the amphibian chytrid fungus Batrachochytrium dendrobatidis (Bd), but the mechanisms for these associations are not entirely clear. Given that pollutants can alter the gut microbiota of hosts, which in turn can affect their health and immune systems, one potential mechanism by which pollutants could increase infection risk is by influencing host‐associated microbiota.
2. Here, we test whether early‐life exposure to the estimated environmental concentration (EEC; 200 μg/L) of atrazine affects the gut bacterial composition of …

Integrated Stress And Community Perceptions: Toward An Understanding Of Human-Cougar Tolerance, Lara Brenner

Graduate Student Theses, Dissertations, & Professional Papers

Evidence suggests that cougars (Puma concolor) are beginning to recolonize their traditional range in the Midwestern and Eastern US, returning to a landscape and a social environment that have changed drastically in a century of absence. Any hope of the cougar’s persistence depends on both human tolerance of their presence and on cougar tolerance of disrupted habitat. In this thesis, we took advantage of diverse cougar policy in place in the Western US to explore variation in human attitudes and acceptability of cougars and in the cougar stress response. We validated a process to identify and extract cortisol …

Stressed Induced Changes In Karenia Brevis Ribosomal Rna, David Scott Jayroe

Master's Theses

Karenia brevis is a toxic marine dinoflagellate that causes harmful algal blooms (HABs), also known as red tides, in the Gulf of Mexico. These blooms are responsible for massive fish kills, shellfish bed contaminations, adverse human health effects, and vast economic loss. For these reasons, extensive research has gone into understanding the mechanisms and dynamics of bloom behavior by studying K. brevis in the field and in the lab. In order to understand higher order bloom behavior and dynamics it is imperative to understand K. brevis at the cellular level. In growing K. brevis in vitro under a variety of …

Seasonal Variation In Corticosterone, Stress Responses, And Physiology In The Northern Cardinal Cardinalis Cardinalis, Benjamin Matthew Duckworth

Master's Theses

Seasonal regulation of the adrenocortical response (e.g., ‘stress response’) appears to be ubiquitous in mid- to high-latitude vertebrates. Northern Cardinals (Cardinalis cardinalis) are a Temperate dwelling passerine of Tropical-descent with a wide species range (tropics to Northern Temperate zones). This species encounters a wide variety of environmental conditions and strong seasonality, however corticosterone (CORT, the main adrenal stress hormone) has not been studied with regards to changes in seasonal levels. I initially analyzed samples from cardinals collected between 2007-2011 from the Lake Thoreau Environmental Research and Educational Center (Hattiesburg, Mississippi, USA). The data suggested seasonal differences of CORT, with higher …