Integrative Biology Commons^™

Development Of A Muc16-Targeted Near-Infrared Antibody Probe For Fluorescence-Guided Surgery Of Pancreatic Cancer, Madeline T. Olson

Theses & Dissertations

Pancreatic cancer (PDAC) is an extremely lethal disease with an overall survival rate of 10%. Surgery remains the only potentially curative treatment option, but resections are complicated by infiltrative disease, proximity of critical vasculature, peritumoral inflammation, and dense stroma. Surgeons are limited to tactile and visual cues to differentiate cancerous tissue from normal tissue. Furthermore, translating preoperative images to the intraoperative setting poses additional challenges for tumor detection, and can result in undetected and unresected lesions. Thus, PDAC has high rates of incomplete resections, and subsequently, disease recurrence. Fluorescence-guided surgery (FGS) has emerged as a method to improve intraoperative detection …

Exploring The Biofilm Of Candida Auris As It Relates To Persistence In Clinical Setting And Susceptibility To Antimicrobials, Bethany Butts

Master of Science in Integrative Biology Theses

The rapid rise of drug-resistant pathogens is a global health concern. Known pathogens are acquiring resistance to many commonly used drugs, while new drug-resistant pathogens are emerging. These resistant pathogens are responsible for many healthcare-associated infections (HAI) [1, 2]. Candida auris is among these emerging drug-resistant pathogens, first described in 2009. The CDC labels C. auris at the highest level of global public health threat [3,4]. Effective methods of decolonization and control of the organisms have yet to be established, as it is resistant to many agents used for microbial control. Recent research has found that C. auris has the …

A Dystrophin Exon‐52 Deleted Miniature Pig Model Of Duchenne Muscular Dystrophy And Evaluation Of Exon Skipping, Yusuke Echigoya, Nhu Trieu, William Duddy, Hong M. Moulton, Haifang Yin, Terence A. Partridge, Eric P. Hoffman, Joe N. Kornegay, Frank A. Rohret, Christopher S. Rogers, Toshifumi Yokota

Genomics and Precision Medicine Faculty Publications

No abstract provided.

Co-Option Of The Yolkless Oocyte Receptor For Crispr/Cas9 May Induce Transient Sterility In Female Drosophila Grimshawi Flies, Bronwyn Miller

LSU New Orleans Theses and Dissertations

Evolutionary developmental biology has historically used generalist model organisms like Drosophila melanogaster. The Hawaiian Drosophilidae radiation, which occurred just 25 million years ago, is an ideal lineage for work in Evo-Devo. Hawaiian flies make up 25% of the world’s Drosophila species and extreme diversity is seen throughout the group. D. grimshawi is a generalist Hawaiian picture-wing fly that has served as a model for the Hawaiian Drosophilidae radiation. However, D. grimshawi’s power and use as a model organism is constrained by our lack of ability to manipulate its genome. In this work, I attempted to edit the genome of …

Computational And Biochemical Characterizations Of Anhydrobiosis-Related Intrinsically Disordered Proteins., Brett R. Janis

Electronic Theses and Dissertations

Anhydrobiosis is the remarkable phenomenon of “life without water”. It is a common technique found in plant seeds, and a rare technique utilized by some animals to temporarily stop the clock of life and enter a stasis for up to several millennia by removing all of their cellular water. If this phenomenon can be replicated, then biological and medical materials could be stored at ambient temperatures for centuries, which would address research challenges as well as enhance the availability of medicine in areas of the world where refrigeration, freezing, and cold-chain infrastructure are not developed or infeasible. Furthermore, modifying crop …

The Evolutionary Origins Of Autism Associated Genes And Their Role In Great Ape Socio-Communicative Behavior, Azeeza Abdulrauf

Master of Science in Integrative Biology Theses

Autism Spectrum Disorder (ASD) is a set of neurological disorders characterized by reduced social interactions and deficits in verbal and nonverbal communication. Although there are no clear genetic markers for ASD, studies have found associations between gene single nucleotide polymorphisms (SNPs) and patients diagnosed with ASD. For this study, the focus is on OXTR, AVPR1A, and FOXP2. OXTR is reported to regulate empathy and stress reactivity while AVPR1A is reported to regulate stress management and territorial aggression, as well as social bonding and recognition. FOXP2 is one of the first genes to be associated with both speech and language recognition …

Mapping Fetch And Diel Movements Of Silver Carp Hypophthalmichthys Molitrix Within Kentucky Lake And Lake Barkley With Gis, Levi Umland

Scholars Week

Title: Mapping Silver Carp movements, relative exposure, and fetch within inland reservoirs using Geographic Information Systems.

Author: Levi G Umland

Invasive species across our country have continued to threaten our native species, compete for habitat via interspecific competition, and have caused economic impacts. Silver Carp (Hypophthalmichthys molitrix), commonly referred to as Asian Carp, have remained in the invasive species spotlight throughout the southern and mid-western states as they have continued to spread. Silver Carp have successfully spread through our rivers and reservoirs, but little is known about Silver Carp movement rates/behavior within reservoirs due to their lower abundance within …

Image-Based Microbiome Profiling Differentiates Gut Microbial Metabolic States, Sarwesh Rauniyar

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

A Comparison Between The Effects Of Empirical And Theoretical Knockouts On The Structure Of Social Networks, Karina Marie Morales, Christopher Stieha

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

How Within-Host Priority Effects Between Specialist And Generalist Pathogens Affect Disease Risk, Jing Jiao, Michael H. Cortez

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Cetacean Acousticwelfare In Wild And Managed-Care Settings: Gaps And Opportunities, Paige E. Stevens, Heather Hill, Jason N. Bruck

Faculty Publications

Cetaceans are potentially at risk of poor welfare due to the animals’ natural reliance on sound and the persistent nature of anthropogenic noise, especially in the wild. Industrial, commercial, and recreational human activity has expanded across the seas, resulting in a propagation of sound with varying frequency characteristics. In many countries, current regulations are based on the potential to induce hearing loss; however, a more nuanced approach is needed when shaping regulations, due to other non-hearing loss effects including activation of the stress response, acoustic masking, frequency shifts, alterations in behavior, and decreased foraging. Cetaceans in managedcare settings share the …

Plastic-Eating Microbes: A New Potential Solution To Waste Mitigation?, Megan Forbes

University Honors Theses

Plastic waste is problematic for marine and terrestrial ecosystems due to the mass production of polyethylene terephthalate (PET) single use plastics. What is unclear is a well-thought-out solution to solve pollution problems that will not further damage the environment. Throughout this literature review, I will investigate the role microbes play in plastic degradation, and if plastic eating microbes are an effective solution to the plastic pollution problem. Polyethylene terephthalate (PET) is the chemical name for polyester. It is a clear, strong, and lightweight plastic that is typically used for food and beverage packaging, and other single-use purposes. The most well …

Assessment Of Non-Saccharomyces Yeast Species As Secondary Fermenters In Beer, Paul J. Clark

Master of Science in Integrative Biology Theses

Secondary fermentation of beer is traditionally used to condition and refine the sensory profile of a beer. During methods such as barrel aging or lagering, the yeast continues to produce flavor-active compounds. Some methods include the intentional inoculation of microorganisms or the utilization of natural consortia associated with a barrel, fruit, or additive for the generation of desired sensory profiles. As unique products increase in popularity, brewers have begun to experiment with secondary fermentation using alternative yeast species as bioflavorants. To assess the viability of an organism’s application to brewing, the physiochemical properties of beer and their impact on the …

The Structural And Functional Role Of Photosensing In Rgs-Lov Proteins, Zaynab Jaber

Dissertations, Theses, and Capstone Projects

Light provides organisms with energy and spatiotemporal information. To survive and adapt, organisms have developed the ability to sense light to drive biochemical effects that underlie vision, entrainment of circadian rhythm, stress response, virulence, and many other important molecularly driven responses. Blue-light sensing Light-Oxygen-Voltage (LOV) domains are ubiquitous across multiple kingdoms of life and modulate various physiological events via diverse effector domains. Using a small molecule flavin chromophore, the LOV domain undergoes light-dependent structural changes leading to activation or repression of these catalytic and non-catalytic effectors. In silico analyses of high-throughput genomic sequencing data has led to the marked expansion …

Bacteriophages Infecting Enterobacter Cloacae To Reduce Bloater Damage In Fermented Cucumbers

Symposium of Student Scholars

Fermented cucumbers are one of the most important fermented vegetables consumed worldwide. During cucumber fermentations, certain undesirable changes may occur. One of such changes is known as bloater defect (hollow cavities in fermented cucumbers), which is primarily caused by gas-producing bacteria including Enterobacter cloacae. Bloater defect lowers product quality and leads to significant economic loss to the pickle industry, and effective preventative methods are needed. Bacteriophages (phages) are highly host-specific bacterial killers. Use of phages to control unwanted bacteria in foods is a promising approach because phages do not change food properties. This research was to isolate, characterize, and …

Initial Assessment Of Potential Relationships Between Plant Communities And The Soil Microbiome In Closed Forest And Longleaf Pine Restoration Sites., Sean Davis, Ian Kennedy

Symposium of Student Scholars

Longleaf pine is an endangered ecosystem characterized by high levels of biodiversity.

Our study took place in the Sheffield Wildlife Management Area located in the Piedmont ecoregion of Georgia in Paulding County. Fifty plots of 10 x 30 m² were setup on south or north facing slopes, some in covered forest, and some in an area actively being restored for the longleaf pine. All trees above 1.37 m were identified and had their diameter measured, and species diversity, relative density, dominance, and frequency were determined. Herbaceous plant cover percentages were recorded in select plots. Soil samples were also collected …

Laryngeal Vocals In Old World Locals: Air Sacs Usage In Bonobos, Chelsea Trenbeath

Symposium of Student Scholars

Except for humans, extant great apes have evolutionarily conserved lateral ventricular air sacs extending from laryngeal saccules. Humans are the only species of Hominidae that lack this anatomical feature attached to the primary vocal apparatus. As we are the only species that produces spoken language, this association has led to hypothesis that the loss of lateral ventricular air sacs was necessary for the evolution of spoken language. However, why these sacs are conserved in all other hominids remains unclear. Computer modeling has indicated that air sacs may increase resonance properties, but there are no data from great apes indicating which …

A 10-Year Post-Analysis Of The Maya Health Toolkit For Medical Providers, Lorenna Garcia-Bochas

Master of Science in Integrative Biology Theses

Researchers have often overlooked America's Indigenous community when conducting health disparities research. In Central and South America, The World Bank 2015 report identifies 774 indigenous groups living today (Davis-Castro, n.d.). One of the oldest known is the Maya people. The Maya have been through various cultural struggles, from the Spanish conquistadors of the past to current anti-indigenous discrimination, violence, and rhetoric. The historical conflict and prejudice have contributed to why many Maya migrate north to the United States for a better opportunity and a more peaceful life for their families. In doing so, they face many health risks to their …

Racial And Socioeconomic Disparities Influencing Obesity Amongst Middle-Aged Women, Morgan Woods

Master of Science in Integrative Biology Theses

Many studies have examined the effects of racial and socioeconomic differences on health-related disparities involving obesity but, few have examined the mental and physical well-being intersection. Obesity is a disease that affects many Americans and has become an epidemic affecting the American population. Obesity, especially morbid obesity, is a risk factor for many other obesity-related diseases such as diabetes, cardiovascular diseases, hypertension, and more. Using the 2018 Healthcare Avoidance Study, differences were found between two racial groups, Caucasian and African-American women. Race was found as a significant factor for both level of physical activity and ideal body image perception

Having A High-Activity Catechol-O-Methyltransferase Allele Is Associated With Elevated Anxiety And Lower Salivary Dehydroepiandrosterone But Also Lower Alpha Amylase In Children With Chromosome 22q11.2 Deletion Syndrome., Jessie Beebe

LSU New Orleans Theses and Dissertations

Chromosome 22q11.2 deletion syndrome (22q11.2DS) results from a hemizygous deletion located on the long arm of chromosome 22. The most common deletion sizes affect between 30 and 90 genes. Individuals with 22q11.2DS may develop serious developmental and psychiatric disorders. The phenotype is highly variable, however, and may be influenced by allelic variation of the retained copies of genes covered by the deletion. I set out to examine the effects of two genes, catechol-O-methyltransferase (COMT) and proline dehydrogenase (PRODH), in relation to anxiety in children and adolescents with 22q11.2DS. Individuals with the major COMT allele (higher activity) have significantly higher anxiety …

The Impacts Of Aging, Sedentarism, And Exercise On Neurotrophic Factor Expression And Innervation In The Heart And The Effects Of Treatment With Α-Cgrp On Heart Function, Gabriel Almeida Alves

Dissertations

Neurotrophic factors (NFs) are important molecules responsible for development, differentiation, regeneration, and maintenance of new and mature neurons. Neurotrophic factors act as neurocytokines and may assist with the regulation of axonal and dendritic arrangements and synaptic plasticity between neurons themselves or with other non-neural target tissues. In this study, we analyze the levels of two NFs: glial cell line-derived neurotrophic factor (GDNF) and nerve growth factor (NGF). Cardiomyocytes produce these neurotrophic factors which assist with the innervation pattern of the heart. The heart is innervated by the two branches of the autonomic nervous system; namely the sympathetic nervous system and …

Evaluating Volatile Organic Compounds For Contact-Independent Antagonism Of Pseudogymnoascus Destructans And Assessing The Impact Of Fungal Pigmentation On Antifungal Susceptibility, Ashley Mcdonald

Master of Science in Integrative Biology Theses

White-nose syndrome (WNS), caused by the psychrophilic fungus Pseudogymnoascus destructans, has been implicated in the massive decline of hibernating bat populations across North America and has led to the listing of impacted species under the US endangered Species Act. Due to the importance of bats to biodiversity and their ecosystem services in agricultural systems, researchers, in conjunction with wildlife management agencies, have been actively exploring disease control methods to mitigate the impact of WNS on susceptible bat species. The accessibility and ecology of bat hibernacula pose unique complications for application of disease management approaches, contributing to the need for …

Quantifying Resource Use Among Insectivorous Bat Species With Overlapping Distributions, Shannon Whitney

Master of Science in Integrative Biology Theses

Bats make up 20% of all mammalian species, are globally distributed, and are the only mammals capable of sustained flight. Bats have adapted to feed on insects, scorpions, aquatic arthropods, mammals, birds, reptiles, amphibians, fish, blood, carrion, fruit, flowers, nectar, pollen, and even seeds and foliage. However, the feeding ecology of these organisms is not well understood. Most bat species in North America rely on the same method of foraging and locomotion. The geographical range and habitats of these bats also commonly overlap. Bat feeding ecology studies have used fecal analysis to identify consumed prey species. Factors such as time …

Analysis Of The Role Of Akirin Interactions With Nuclear Body Proteins During Myogenesis, Laura Yorke

Master of Science in Integrative Biology Theses

Akirin is a highly conserved, small nuclear protein of indeterminate structure ubiquitously expressed in non-fungal eukaryotic species. It was first discovered in the innate immune response, but many other functions have since been found, including embryonic muscle patterning and myogenesis. Animals with either nonfunctional or missing Akirin have aberrations in embryonic muscle patterning, along with other defects. Akirin interacts with transcription factors such as Twist to coordinate development through interfacing between Twist and other complexes, such as the Brahma Chromatin Remodeling Complex (BRM). Therefore, Akirin likely plays a general role in transcription during early development, interfacing with other transcriptional machinery …

Late Embryogenesis Abundant Proteins And Mitochondrial Membranes From An Animal Extremophile: Insights Into Severe Water Stress, John Marcus Anderson

LSU Doctoral Dissertations

This dissertation focuses on two major topics germane to mechanisms by which animals tolerate extreme water stress. First, the impact of transgenic expression of late embryogenesis abundant proteins (LEA) on water stress tolerance of the fruit fly Drosophila melanogaster was investigated. The overall aim was to extend current understanding of the protective properties of LEA proteins documented with isolated cells to a desiccation-sensitive organism during exposure to drying and hyperosmotic stress. Four lines of D. melanogaster were created that expressed transgenes encoding selected LEA proteins originally identified in embryos of the extremophile Artemia franciscana. After 80% tissue water loss, …

Yakety Sacs: Laryngeal Air Sac Usage In Great Apes, Chelsea Trenbeath

Master of Science in Integrative Biology Theses

Except for humans, extant great apes have evolutionarily conserved lateral ventricular air sacs extending from laryngeal saccules. Humans are the only species of Hominidae that lack this anatomical feature attached to the primary vocal apparatus. As we are the only species that produces spoken language, this association has led to the hypothesis that the loss of lateral ventricular air sacs was necessary for the evolution of spoken language. However, why these sacs are conserved in all other hominids remains unclear. Computer modeling has indicated that air sacs may increase resonance properties, but there are no data from great apes indicating …

Potential Therapeutic Effects Of Snake Venom Components On Pain Management In Rheumatoid Arthritis Patients, Maggie Metzger

University Honors Theses

This paper reviews the existing literature on the uses of snake venom in the treatment of rheumatoid arthritis and pain management. Venom from the families Elapidae and Viperidae have been shown to have anti-inflammatory and analgesic effects. The analgesic findings of research on arthritis murine models are discussed, and the existing uses of snake venom in medicine to suggest the therapeutic potential of venom in the pain management of rheumatoid arthritis are taken into account. Snake venom has anti-inflammatory effects by reducing levels of pro-inflammatory cytokines and increasing levels of anti-inflammatory cytokines. Additionally, snake venom can reduce structural damage from …

Temperature Regulation Of Plant Hormone Signaling During Stress And Development, Christian Castroverde, Damaris Dina

Biology Faculty Publications

Global climate change has broad-ranging impacts on the natural environment and human civilization. Increasing average temperatures along with more frequent heat waves collectively have negative effects on cultivated crops in agricultural sectors and wild species in natural ecosystems. These aberrantly hot temperatures, together with cold stress, represent major abiotic stresses to plants. Molecular and physiological responses to high and low temperatures are intricately linked to the regulation of important plant hormones. In this review, we shall highlight our current understanding of how changing temperatures regulate plant hormone pathways during immunity, stress responses and development. This article will present an overview …

Manipulation Of The Microbiome In The Gut Of The Fruit Fly To Alleviate Cadmium Bioaccumulation, Natasya Tamba

Symposium of Student Scholars

Cadmium (Cd) poisoning contributes to severe bodily detriments characterized by kidney failure, osteomalacia, and osteoporosis, otherwise known as Itai-Itai disease. The ingestion of cadmium contaminated foods primarily causes these conditions. There are no treatments to remove cadmium once absorbed, resulting in bioaccumulation. In this study, we are developing a method to manipulate the community of microorganisms (microbiome) living within the gastrointestinal (GI) tract of the fruit fly (Drosophila melanogaster), intending to use microorganisms to reduce the availability of ingested cadmium.

The biodiversity of microorganisms within the GI tract is responsible for metabolizing substances that the body cannot perform. …

Evolution Of Extreme Habitat Specialists In The Dark: Cavefishes And Anglerfishes, Pamela Hart

LSU Doctoral Dissertations

Extremophilic organisms are fascinating in that they thrive in physiologically demanding environments. Extreme habitats include subsurface caves and the deep sea, which share several qualities like low light and cold temperatures. Extremophiles have adapted bizarre characteristics that allow them to thrive in such harsh environments including eye and pigment loss (cavefishes) and development of a lure in anglerfishes. These traits are integral to the health and fitness of these organisms, yet the evolutionary history of these traits is unclear.

Cave adaptation has evolved repeatedly across the Tree of Life, famously leading to eye degeneration and loss, yet its macroevolutionary implications …