Integrative Biology Commons^™

Infrared Detectability Of Whale Species And Behaviors To Enhance Automatic Detection For Real-Time Vessel Strike Avoidance, Laura J. Howes

Graduate Masters Theses

Real-time detection of large whales at sea is critical for mitigating serious injury and mortality from ship strikes and other human activities such as seismic surveys, especially for endangered species such as the critically endangered North Atlantic Right Whale (Eubalaena glacialis). Traditional marine mammal observers are limited by environmental conditions and human factors, necessitating a robust, 24-hour solution. This study investigates the efficacy of a novel onboard detection tool infrared (IR) technology to detect whale blows and body parts. While IR is a promising advancement, its performance under varying sea conditions (e.g., fog, humidity) and its ability to …

Marine Heatwaves And Fish Reproduction: Endocrine Responses In The Eurythermal Sheepshead Minnow (Cyprinodon Variegatus), Isabel Villafuerte

Master's Theses

Marine heatwaves associated with climate change are increasing in frequency and severity, posing significant threats to fish populations in shallow habitats such as estuaries and salt marshes. Because temperature is a primary regulator of fish reproduction, understanding how heatwaves affect reproductive physiology is critical for anticipating the biological consequences of ongoing climate change for coastal fishes. Here we examined how simulated heatwave conditions affect the reproductive hypothalamic-pituitary-gonadal (HPG) endocrine axis in the estuarine sheepshead minnow (Cyprinodon variegatus), a eurythermal fish well suited to studying thermal stress responses in estuarine environments. Adult sheepshead minnows acclimated to a daily oscillating …

Evaluating The Commercial Aquaculture Potential Of The Native Pacific Littleneck Clam (Leukoma Staminea) As A Climate Resilient Alternative To The Non-Native Manila Littleneck Clam (Ruditapes Philippinarum)., Skylar M. Wuelfing

Master's Theses

The California shellfish industry’s near-exclusive reliance on the non-native Manila littleneck clam (Ruditapes philippinarum) creates an economic vulnerability to climate-driven disturbances, which can cause mortality events that eradicate up to 80% of stocks. This singular reliance on an introduced species highlights a need for the evaluation of native alternatives like the Pacific littleneck clam (Leukoma staminea). Our goal was to assess the feasibility of using the native Pacific littleneck in commercial aquaculture by comparing their performance to the non-native Manila littlenecks across the following conditions: 1) a five-month field outplant trial at high and low intertidal …

Geographic Variation In Proteomic Responses To Ocean Acidification In A Cold-Water Coral (Balanophyllia Elegans), Keana A. Richmond

Cal Poly Humboldt theses and projects

In the face of a rapidly changing climate, assessing organismal responses to future stressors in the context of current, natural exposure to stress could provide key insights to understanding marine ecosystem resilience. I used Balanophyllia elegans, a cold-water, solitary, azooxanthellate coral as a model to better understand how varying oceanographic conditions across its geographic range have shaped its ability to tolerate and potentially adapt to current and future ocean acidification conditions. I collected B. elegans individuals from four sites across 2,500km of their range and subjected them to two pH treatments to investigate site-specific protein expression in response to …

Hybridization And Introgression In Marine Invertebrates: Population Genomics Of Phymactis Papillosa And Asterias Sea Stars, Christopher J. Dookie

Dissertations and Theses

Hybridization and introgression are evolutionary significant processes in marine invertebrates (Sanford & Kelly,2011) yet genome-wide investigations of gene flow in these taxa remain limited relative to vertebrate systems (Marchi et al., 2021). This thesis presents genomic analyses of hybridization and introgression in two marine invertebrates occupying contrasting oceanographic settings and characterized by contrasting genomic datasets: restriction-site associated DNA sequencing (RADseq) SNP data for the eastern Pacific sea anemone Phymactis papillosa and whole genome sequencing (WGS) data for the North Atlantic sea stars Asterias rubens and Asterias forbesi. For each system, I applied coalescent-based species tree inference using CASTER (Zhang …

Trophic Roles And Climate-Driven Vulnerability For Benthic Invertebrates At Artificial Reefs In The Northern Gulf Of Mexico, Amanda Kirkland

LSU New Orleans Theses and Dissertations

Artificial reefs are deployed to enhance marine habitats and fisheries, yet their role in supporting secondary production and their vulnerability to climate stressors remain understudied. This dissertation investigates the trophic ecology and climate sensitivity of benthic invertebrates at artificial reefs in the northern Gulf of Mexico. Chapter 1 assessed reef food webs using stable isotopes from fishes, invertebrates, and primary producers across six reefs and two seasons. Mixing models showed that most fishes rely on benthic reef carbon, while suspension feeders consume plankton and sediment microphytobenthos. Isotopic niche analysis revealed functional diversity and seasonal shifts in δ¹³C and δ³⁴S, though …

Metabolic Transitions In Embryos Of Artemia Franciscana: Regulation And Consequences For Oxidative Stress, Daniel Anthony Arabie

LSU Doctoral Dissertations

Embryos of Artemia franciscana survive harsh conditions in diapause and anoxia-induced quiescence for years by undergoing deep metabolic transitions. I investigated the relationship between phosphorylation of pyruvate dehydrogenase (PDH) and carbon flux to the tricarboxylic acid (TCA) cycle during metabolic depression. The difference in catalytic activity of the PDH complex (PDC) in maximally phosphorylated and dephosphorylated samples was 16.5-fold. Western blot analysis confirmed the changes in phosphorylation of PDH. Analysis of PDH expression in diapause and post-diapause embryos via phospho-PDH showed no significant differences. Investigation of post-diapause embryos under anoxia showed that phosphorylation of PDH decreased and activated the enzyme. …

Florida Statewide Coastal Restoration Guide, Melinda J. Donnelly, Matthew Martin, Christen Fleming, Kelly Kibler, Linda Walters, Patrick Shearer, Lauren Jennison, Erika Lin, George Zaragoza

Faculty Scholarship and Creative Works

The Statewide Coastal Restoration Guide (SCRG) is a comprehensive planning resource developed through a collaboration between Florida Department of Environmental Protection (FDEP), University of Central Florida (UCF), and Resource Environmental Solutions (RES) to strengthen and unify shoreline restoration efforts across Florida. With over 4,500 miles of estuarine shoreline, Florida’s coasts are vital to the state’s ecological health, economic prosperity, and community resilience. However, they face increasing threats from sea level rise, stronger storms, erosion, habitat degradation, and development. The SCRG responds to these challenges by providing science-based, non-regulatory guidance for implementing resilient, nature-based restoration strategies that protect both ecosystems and …

Prochlorococcus Death By A Ubiquitous Zooplankton Filter Feeder, Avery Harman

University Honors Theses

Prochlorococcus is a globally abundant cyanobacterium that can help reveal the ecology of microorganisms in the ocean. Specifically, mortality by zooplankton such as appendicularians is globally important and Prochlorococcus can help reveal some of the choices made around feeding for this ubiquitous filter feeder. Two ecotypes of Prochlorococcus were introduced to appendicularians to determine if appendicularian feed selectively between these ecotypes. Further, a single ecotype of Prochlorococcus was fed to appendicularians over a variety of life stages to determine if retention rate changed with appendicularian life stage. My results reveal that there is no significant selection between the two Prochlorococcus …

Phenotypic Plasticity In Pigmentation: Investigating Coloration Patterns In Anablepsoides Hartii Population Responses To Environmental Variation, Jasdeep Kaur

2024 Spring Honors Capstone Projects - Archive

In this study, we investigated the color-changing capabilities of Trinidadian killifish, Anablepsoides hartii, through exposure to varied color conditions over defined periods of time. Our experiment aims to understand adaptive responses when killifish are subjected to different color backgrounds, exploring their mechanisms to change color. By subjecting the species to different environmental color variations, we sought to discern color change patterns, which could indicate adaptive responses related to camouflage, stress, behavior clues, and physiological adjustments. Carefully controlling environmental factors, we monitored and documented the fishes’ color changes, aiming to provide insights into the interplay between fish’s developmental plasticity and its …

Novel Microbiological Medium Developed For The Isolation Of Bacteria Associated With Estuarine Anemones, Parker K. Lund, Catalina Cuellar-Gempeler

IdeaFest: Interdisciplinary Journal of Creative Works and Research from Cal Poly Humboldt

Out of the nearly one trillion species of microbiota estimated to inhabit Earth only ten thousand have been cultured in the laboratory. Culturing continues to play a vital role in determining the physiology and ecologic function of individual bacteria in microbial communities and for microbes associated with host organisms one of the major challenges is developing microbiological media that mimics the bacteria’s natural environment enough to promote growth. Here, we target bacteria associated with the estuary anemones Diadumene lineata and Metridium senile by developing a novel medium that uses anemone tissue as the sole source of nutrients. We further measured …

Parental Environnement Influences Offspring Gene Expression In The Olympia Oyster, Ostrea Lurida., Mark S. Yeats

LSU Master's Theses

The Olympia Oyster is the only native oyster species on the Pacific coast of North America. Seasonality in water temperatures across this threatened species’ broad and fragmented range has been linked to its development, reproductive timing, reproductive activity, growth, and survival rates. Recent evidence suggests parental environments can have carry-over effects on larval growth and respiration rates in response to temperature. In this study, we utilized transcriptomic methods to identify the underlying mechanisms of this cross-generation plasticity. Sequence libraries of adult ctenidia tissue and pooled larval replicates were extracted and spawned from hatchery raised oysters of the same parentage immediately …

The Cetacean Sanctuary: A Sea Of Unknowns, Jason N. Bruck

Faculty Publications

Housing cetaceans in netted sea pens is not new and is common for many accredited managed- care facilities. Hence, the distinction between sanctuary and sea pen is more about the philosophies of those who run these sanctuary facilities, the effects of these philosophies on the animals’ welfare, and how proponents of these sanctuaries fund the care of these animals. Here, I consider what plans exist for cetacean sanctuaries and discuss the caveats and challenges associated with this form of activist-managed captivity. One goal for stakeholders should be to disregard the emotional connotations of the word “sanctuary” …

Corals In Crisis: A Transcriptomic Investigation Of Stony Coral Tissue Loss Disease, Kelsey M. Beavers

Biology Dissertations - Archive

Coral reefs face existential threats due to climate change and human activities. The combined effects of ocean warming, overfishing, pollution, and habitat destruction have fundamentally altered the functionality of coral reefs and are driving a drastic increase in the prevalence and severity of coral disease outbreaks. Stony coral tissue loss disease (SCTLD) has emerged one of the most devastating disturbances to Caribbean reef ecosystems on record. Despite notable progress, the cellular mechanisms driving SCTLD pathogenesis remain poorly understood. To address this issue, my dissertation investigates the gene expression, histopathology, immune protein activity, and symbiont community dynamics involved in SCTLD progression …

Quantifying The Role Of Water Quality On Nitrogen Cycling In A Trophic Estuary, Kayla Gonzalez-Boy

Symposium of Student Scholars

Jobos Bay Estuary is an intertidal, tropical estuary located in southern Puerto Rico. The estuary covers about 12 km² and has a variety of habitats, such as seagrass beds, mangroves, mud flats, and coral reefs, which play important roles in sediment trapping and water quality maintenance. Seagrasses also serve as nursery and feeding grounds and provide shelter for macrofauna. Currently, the role of seagrasses and water quality on nitrogen (N) cycling in trophic estuaries is not well constrained. Understanding variations in sediment-based effects on N cycling rates and transformations, and how they are associated with water quality, is an …

Effects Of Intertidal Position On The Response To Oxygen And Desiccation Stress In The Common Acorn Barnacle, Balanus Glandula, Megan M. Dotterweich

Master's Theses

Sessile invertebrates in the rocky intertidal experience intermittent periods of air exposure due to tidal flux, presenting risks of temperature extremes, hypoxia, nutrient limitation, and most dangerously, desiccation. Microscale variation in severity and frequency of these risks is widely dependent on vertical position within the intertidal zone. Common acorn barnacles (Balanus glandula) have a wide vertical distribution in the intertidal, creating large differences in microhabitat between the highest and lowest individuals in the population. This study set out to explore whether tidal position dependent differences exist in the response to oxygen and desiccation stress in B. glandula. …

A Quieter Ocean: Experimentally Derived Differences In Attentive Responses Of Tursiops Truncatus To Anthropogenic Noise Playbacks Before And During The Covid-19-Related Anthropause, Paige E. Stevens, Veda Allen, Jason N. Bruck

Faculty Publications

The effects of anthropogenic noise continue to threaten marine fauna, yet the impacts of human-produced sound on the broad aspects of cognition in marine mammals remain relatively understudied. The shutdown of non-essential activities due to the COVID-19-related anthropause created an opportunity to determine if reducing levels of oceanic anthropogenic noise on cetaceans affected processes of sensitization and habituation for common human-made sounds in an experimental setting. Dolphins at Dolphin Quest Bermuda were presented with three noises related to human activities (cruise ship, personal watercraft, and Navy low-frequency active sonar) both in 2018 and again during the anthropause in 2021 via …

Responses Of A Pine Flatwoods Specialist Treefrog To Prescribed Fire, Ian Biazzo

Electronic Theses and Dissertations, 2020-2023

Coarse outcomes of prescribed fires are well-understood, but fine scale impacts on many assemblages are still unknown. When fire approaches, animals escape to unburned areas, hide in place, or are killed by fire. Treefrogs are unique in their use of trees for most of their adult nonbreeding activities. This reliance on uplands and three-dimensional landscape composition makes them a great model to study prescribed fire impacts. We focused analyses on an upland flatwoods specialist comprising 99% of our captures, the pinewoods treefrog Dryophytes femoralis. We assessed this species' responses to prescribed fire using two separate before-after-control-impact replicated field experiments in …

Host-Microbe Interactions In Non-Native Estuarine Anemones: Biogeography And Temperature, Parker K. Lund

Cal Poly Humboldt theses and projects

Non-native species are increasing in prevalence around the world, resulting in negative economic and ecological impacts. However, the broad distributions of non-native species also offer a system for investigating the response of host-associated microbial communities to environmental factors across a range of ecological scales. At the broadest scale, I investigated the geography of microbial communities in the non-native estuarine anemone Diadumene lineata on the west coast of the United States of America. Across latitudes, microbial community composition was very similar and displayed a high percentage of Klebsiella spp. at all sites. However, the communities in California tended to exhibit higher …

Are Biodiverse Communities More Resistant To Invasion? A Case Study With Marine Fouling Communities, Taylor Robert Bruntil

Cal Poly Humboldt theses and projects

The theory of biotic resistance predicts that more diverse communities should be less susceptible to invasion by novel species, but given the opposing results of multiple observational and experimental studies in marine systems, it is unclear how changes in environmental conditions can affect invasion success in communities that differ in diversity. I used marine fouling communities to test how the diversity of the species present in an initial community (hereafter termed “resident species”) affected the establishment and growth of all species not present in the initial communities (hereafter termed “novel species”) at two locations at the Eureka Public Marina, in …

Caribbean Reef-Building Coral-Symbiodiniaceae Network: Identifying Symbioses Critical For System Stability In A Changing Climate, Shaman Patel

All HCAS Student Capstones, Theses, and Dissertations

Increasing global ocean temperatures and frequency of marine heatwaves pose dire consequences for coral reefs. High temperatures often lead to disruptions in coral symbiosis resulting in coral bleaching, increasing the mortality of corals. However, corals can potentially avoid bleaching peril by associating with thermally tolerant symbionts. Here we provide a tool for understanding symbiosis network stability of Caribbean reef-building corals. We created a network of Caribbean hermatypic corals and their associated Symbiodiniaceae phylotypes. A bleaching model was applied to this network to test for resilience and robustness (R50) to thermal stress. It was also layered with trait data for coral …

Larval Fish Export In Response To Varying Compensating Gate Discharge At The Sault Rapids, St. Marys River, Signe F.K. Vandrunen

All NMU Master's Theses

The St. Marys River (SMR) forms the border between Michigan, USA and Ontario, Canada connecting lakes Superior and Huron. Discharge is controlled by a compensating gate system upstream of the SMR rapids. Because spring and summer discharge fluctuates widely, resource managers required information on how flow variability influences larval fish drift. I estimated annual larval fish export in relation to variable discharge during 2018-2021 and compared results to published habitat model predictions. Mean discharge and water temperature were lowest in 2021 and highest in 2019. Water warming rates during high discharge differed from low discharge trends. Across years, I identified …

Evaluating The Impact Of Intersecting Research And Outreach Marine Science Programs On Elementary And Undergraduate Students, Randi J. Sims

All Theses

Climate change is one of the most destructive forces our ocean is currently experiencing. Despite this, many students are not taught the basics of climate change science and ocean literacy in public school systems. My work seeks to combat these deficits through educational experiences in marine science for undergraduate and local elementary students through three studies incorporating marine-science based research and outreach. (1) Through undergraduate marine science research and outreach, students enrolled for long durations or with positive mentorship increased their conceptual understanding of marine science concepts, altered their attitudes towards climate change and science, and were more likely to …

Consequences Of Gulf Of Mexico Estuarine Tropical Cyclone Disturbance Regimes For Fish Assemblage Dynamics Conditionally Depend On Life-History Traits And Niche Characteristics, Stephen Edward Potts

LSU Doctoral Dissertations

Tropical cyclones (TC) are intense, localized disturbances that can potentially cause extensive damage to ecosystems. This dissertation is motivated by a need for interdisciplinary study of TC-ecosystem dynamics with applications to ecology, geography, and analytical methodology. I explore concept linkages between TC dynamics and ecosystems to characterize TC-ecosystem relationships as ecological disturbance regimes. This dissertation tests hypotheses and relationships of TC effects on estuarine fish assemblages in the north-western Gulf of Mexico. I explore concepts in TC-ecosystem relationships to link biotic dynamics with physical disturbance.

In Chapter 2, I compared spatial performance of generalized linear models (GLMs) and generalized additive …

Repeated Boat Noise Exposure Damages Inner Ear Sensory Hair Cells And Decreases Hearing Sensitivity In Atlantic Croaker (Micropogonias Undulatus), Gina A. Badlowski

LSU New Orleans Theses and Dissertations

No abstract provided.

Role Of Biological Clocks In Ant Behavioral Plasticity And Parasitic Manipulation Of Ant Behavior, Biplabendu Das

Electronic Theses and Dissertations, 2020-2023

Living organisms exhibit daily rhythms as a way to anticipate predictable fluctuations in their environment. Such daily rhythmicity is the phenotypic outcome of oscillating genes and proteins, driven by an endogenous biological clock. Clock-controlled behavioral rhythms are inherently "flexible" since their phase, amplitude, and period can change throughout an animal's life hallmarked by changes in so-called chronotype. How this inherent plasticity of clock-controlled rhythms is linked to plasticity of behavior is still an open question in biology. Characterizing the various mechanistic links between plasticity of the animal clock and behavioral state will not only shed light on the molecular underpinnings …

Juvenile Dispersal And Genetic Connectivity In The Sea Turtle 'Lost Years', Katrina Phillips

Electronic Theses and Dissertations, 2020-2023

Juvenile dispersal is a life history strategy found across taxa and habitat types. In this dissertation, I examine juvenile dispersal in an evolutionary context and explore the conditions that support high dispersal, which necessitates subsequent ontogenetic habitat shifts. I use a sea turtle study system to evaluate the genetic and behavioral patterns associated with juvenile dispersal and ontogenetic shifts in marine environments. Specifically, I focus on four sea turtle species found in the northeastern Gulf of Mexico in the early life stage known as the 'lost years': green turtles (Chelonia mydas), Kemp's ridleys (Lepidochelys kempii), loggerheads (Caretta caretta), and hawksbills …

Histological Comparison Of Shark Dermis Across Various Ecomorphologies, Olivia Schuitema

UNF Graduate Theses and Dissertations

The integument plays essential roles in the structural support, protection, and hydrodynamic capability among fishes. Most shark skin research has been done on the external epidermal layer containing the dermal denticles, while the larger dermis layer has been mostly ignored. The dermis layer in sharks is composed of two layers, the upper stratum laxum and the lower stratum compactum, holding supportive collagen and elastin fibers. There may be morphological and compositional differences in the dermis layers across various species of sharks, although the extent of such is unknown. These potential differences may be represented by various dermal thicknesses and different …

Cetacean Acousticwelfare In Wild And Managed-Care Settings: Gaps And Opportunities, Paige E. Stevens, Heather Hill, Jason N. Bruck

Faculty Publications

Cetaceans are potentially at risk of poor welfare due to the animals’ natural reliance on sound and the persistent nature of anthropogenic noise, especially in the wild. Industrial, commercial, and recreational human activity has expanded across the seas, resulting in a propagation of sound with varying frequency characteristics. In many countries, current regulations are based on the potential to induce hearing loss; however, a more nuanced approach is needed when shaping regulations, due to other non-hearing loss effects including activation of the stress response, acoustic masking, frequency shifts, alterations in behavior, and decreased foraging. Cetaceans in managedcare settings share the …

A Brain-Infecting Parasite Impacts Host Metabolism Both During Exposure And After Infection Is Established, Lauren E. Nadler, Erik Bengston, Erika J. Eliason, Cameron Hassibi, Siri H. Helland-Riise, Ida B. Johansen, Garfield T. Kwan, Martin Tresguerres, Andrew V. Turner, Kelly L. Weinersmith, Øyvind Øverli, Ryan F. Hechinger

Marine & Environmental Sciences Faculty Datasets

1. Metabolic costs associated with parasites should not be limited to established infections. Even during initial exposure to questing and attacking parasites, hosts can enact behavioural and physiological responses that could also incur metabolic costs. However, few studies have measured these costs directly. Hence, little is known about metabolic costs arising from parasite exposure.
2. Further, no one has yet measured whether and how previous infection history modulates metabolic responses to parasite exposure.
3. Here, using the California killifish (Fundulus parvipinnis) and its brain-infecting parasite (Euhaplorchis californiensis), we quantified how killifish metabolism, behaviour, and osmoregulatory phenotype changed upon acute …