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- Background DNA (3)
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Articles 1 - 8 of 8
Full-Text Articles in Forensic Biology
Effect Of Food Resource, Preservation Method, And Preservation Duration On Larval Size Of Forensically Important Fly Species (Order: Diptera), John Zukowski
Student Theses
Forensic entomology is a vital discipline that can aid in the proceedings of criminal cases. Larval age and development are important aspects to consider when utilizing blow flies (Family: Calliphoridae) for estimates pertaining to the initial time of colonization (TOC), which contributes to postmortem interval (PMI) estimations. Thus, it is important to understand the factors that influence larval size and development, since larval size is commonly used as a proxy for age. Larvae of three species of flies, Lucilia sericata (Meigen), Calliphora vicina (Robineau-Desvoidy), and Phormia regina (Meigen), were reared on five different food resources. Specimens of L. sericata and …
Exploring Biogeographical Ancestry Inference Of New York Residents Using A 45 Indel Panel, Amanda Martinez
Exploring Biogeographical Ancestry Inference Of New York Residents Using A 45 Indel Panel, Amanda Martinez
Student Theses
Biogeographical ancestry (BGA) inference is an important tool in forensic science, providing supplemental information when comparison/reference DNA is not available for traditional DNA analyses. Insertion/deletion polymorphisms (INDELs) have been used in the last decade as ancestry-informative markers (AIMs) for BGA inference because they can be easily adapted to forensic-type samples and are determined using low cost and easy processing methods. Limited research has attempted to estimate ancestry and admixture in United States (U.S.) populations. This study evaluated the effectiveness of a 45- and 21- INDEL panel to measure population admixture proportions of four different origins (African, European, East Asian and …
Indirect Dna Transfer To Duct Tape Rolls Stored In Cars, Yuching Chung
Indirect Dna Transfer To Duct Tape Rolls Stored In Cars, Yuching Chung
Student Theses
This project investigated the accumulation of DNA on duct tape rolls stored in cars. Specifically, we analyzed samples recovered from three areas (the inside, outside, and flat sides) of unused duct tape stored under the driver's seat and trunk of cars for seven days. Twelve volunteers were recruited, resulting in 64 DNA samples from 12 cars. We evaluated DNA quantities and STR genotyping outcomes after amplification with the GlobalFiler™ kit. On average, the DNA yields from under the driver's seat were higher than from the trunk, but the difference was not significant. The inside of the tape roll yielded significantly …
Characterization Of Dna Background On Duct Tape Stored In Domestic Environments, Elaan A. Lukasiewicz
Characterization Of Dna Background On Duct Tape Stored In Domestic Environments, Elaan A. Lukasiewicz
Student Theses
With lower detection limits for forensic DNA analysis, it is important to have a better understanding of background DNA levels unrelated to the crime to facilitate accurate data interpretation. Duct tape is used in crimes, e.g. to bind victims and seal drug packaging, and may be tested for DNA. To determine DNA background levels, twelve volunteers were recruited to donate a used roll of duct tape from their home. The duct tape was divided into three areas: inside, outside, and sides of the roll, and swabbed for DNA. The average concentration of DNA was determined for the inside (0.0723 ng/µL …
Str Analysis Performance Comparison For Two Capillary Electrophoresis Instruments: The Seqstudio And The 3500 Genetic Analyzers, Yana Leichyk
Student Theses
This study compares the performance of two capillary electrophoresis instruments—the SeqStudio™ Genetic Analyzer and the 3500™ Genetic Analyzer—for forensic short tandem repeat (STR) analysis. Using the GlobalFiler STR kit and certified reference material (SRM 2391d), five key performance metrics were evaluated: sizing precision, signal reproducibility, detection sensitivity, mixture resolution, and overload tolerance. STR profiles were generated from single-source and mixture samples with DNA inputs ranging from 500 pg to 15.6 pg. Both instruments met the < 0.15 bp sizing precision requirement for forensic casework. Signal reproducibility tests showed that the SeqStudio often produced higher average RFU values, but with greater variability, while the 3500 demonstrated more stable signal performance across days and samples. Mixture detection was effective on both platforms up to 1:10 ratios. Dropout of minor alleles began to appear at 1:20 ratios, especially in green and yellow dye channels, on both instruments. Sensitivity studies confirmed full profile recovery down to 62.5 pg, with increasing allelic and locus dropout at lower inputs. Enhanced injection voltage (1.5 kV) did not improve low-template performance. In overload testing (1–5 ng input), the 3500 maintained a consistent signal output, while the SeqStudio exhibited signal suppression at 5 ng, likely due to internal normalization. The SeqStudio exhibited better spectral overlap compensation, with fewer occurrences of spectral bleed-through artifacts. These findings support the use of both platforms in forensic casework, while emphasizing the importance of platform-specific calibration and analytical threshold settings for optimal performance.
Dna Extraction And Species Determination Of Historic Museum Pelt Samples, Selin Ates
Dna Extraction And Species Determination Of Historic Museum Pelt Samples, Selin Ates
Student Theses
The identity of the fur covering the sculpture named Object in the Museum of Modern Art has remained a mystery since its creation in 1936 by Meret Oppenheim. DNA testing can possibly provide an answer, but to preserve the integrity of the sculpture, only a very small piece of the fur can be sampled. Prior to testing Object, testing using museum samples was performed to determine whether DNA of a sufficient quantity and quality can be extracted and sequenced. Several DNA extraction and sample washing methods were tested to examine the effects on the DNA of museum pelts aged …
A Qualitative Nmr And Histological Analysis Of Adipocere Formation In Different Aqueous Environments Using Sus Scrofa Models, Mia M. Sorrentino
A Qualitative Nmr And Histological Analysis Of Adipocere Formation In Different Aqueous Environments Using Sus Scrofa Models, Mia M. Sorrentino
Student Theses
Adipocere is a wax-like crust that has the potential to form over corpses in moist, humid, and anaerobic environments, preserving them in a process similar to that of mummification. The substance can be identified within 3-6 months postmortem and is characterized by the ratio of saturated fatty acids to unsaturated fatty acids. Palmitic acid and stearic acid are the most abundant in comparison to myristic and oleic acids. The goal of this study was to investigate factors affecting adipocere formation in different aqueous environments. Pork belly samples were submerged in water samples collected from different places around New York City …
Passive Dna Transfer To Fidget Blasters Stored In Cars, Rachel Chan
Passive Dna Transfer To Fidget Blasters Stored In Cars, Rachel Chan
Student Theses
This study evaluated the amount of detectable DNA accumulated on clean plastic fidget blasters, used to simulate firearms, over a seven-day storage period inside a vehicle. Twelve volunteers were asked to place one fidget blaster each in the glove box and underneath the driver’s seat from behind. After the seven-day period, the volunteers returned the fidget blasters, donated a reference sample, and filled out a questionnaire. The grip, slide, and trigger were swabbed for DNA and extracted. The extracts were quantified to determine the concentration of human DNA, and if the samples met the PCR threshold to be genotyped. The …