Biology Commons^™

Darwin At The Drugstore? Testing The Biological Fitness Of Antibiotic Resistant Bacteria, Alan L. Gillen, Sarah Anderson

Faculty Publications and Presentations

The issue of antibiotic resistance in bacteria is growing. For example, methicillin-resistant Staphylococcus aureus (MRSA) is a leading human pathogen. New strains of MRSA are resistant to methicillin and numerous other antibiotics, placing the patient in significant danger. Indeed, the Centers for Disease Control revealed in 2007 that the number of deaths due to MRSA exceeded that of AIDS in the United States. It is no wonder that antibiotic resistance is one of the most significant and important health care issues. The ability of bacterial pathogens to evade traditional antibiotics is faster than the drug makers’ ability to make new …

The Reproductive Ecology Of Graptemys Geographica In The Central Canal, Kati Keppen Rush

Undergraduate Honors Thesis Collection

Life for all organisms involves carefully managing a limited amount of resources. In many cases these resources affect how organisms Jive on a daily basis. In addition to competition from other species, organisms face competition within their own species, populations, and niches. Those individuals that manage their resources effectively will increase their chances of survival, reproduction, and the continuation of their genes in the gene pool. Studies that focus on life history characteristics of organisms aim to understand the mechanisms used by organisms to increase the chances that their genes will remain in thegenepoolpasttheirown Iifetime.Thegoal ofthesemechanisms istoensuresurvival and maximize reproduction. …

Characterization Of The Interaction Between The Fanconi Anemia (Fa) D2 Protein And Proliferating Cell Nuclear Antigen (Pcna), Carolyn Schmiedel

Senior Honors Projects

No abstract provided.

Reproductive Biology Of The Coyote (Canis Latrans): Integration Of Behavior And Physiology, Debra Anne Carlson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Wild Canis species possess a unique suite of reproductive traits including social monogamy, copulatory lock/tie, and biparental care. Females are seasonally monestrous and experience an obligatory pseudopregnancy after spontaneous ovulation. While these characteristics have been ascribed to coyotes, an integrated profile of behavior and physiology has not yet been described. In this study, temporal correlations between steroid hormone levels and socio-sexual mating behaviors were documented, as were changes in vaginal epithelium. Pseudopregnancy was compared to pregnancy by contrasting hormone (progesterone, estradiol, prolactin and relaxin) profiles of unmated females to patterns obtained in alternate years when they bred. Meanwhile, social interactions …

Peer Review In An Undergraduate Biology Curriculum: Effects On Students’ Scientific Reasoning, Writing And Attitudes, Briana Eileen Timmerman

Faculty Publications

Scientific reasoning and writing skills are ubiquitous processes in science and therefore common goals of science curricula, particularly in higher education. Providing the individualized feedback necessary for the development of these skills is often costly in terms of faculty time, particularly in large science courses common at research universities. Past educational research literature suggests that the use of peer review may accelerate students’ scientific reasoning skills without a concurrent demand on faculty time per student. Peer review contains many elements of effective pedagogy such as peer-peer collaboration, repeated practice at evaluation and critical thinking, formative feedback, multiple contrasting examples, and …

Proper Cellular Reorganization During Drosophila Spermatid Individualization Depends On Actin Structures Composed Of Two Domains, Bundles And Meshwork, That Are Differentially Regulated And Have Different Functions, Tatsuhiko Noguchi, Marta Lenartowska, Aaron D. Rogat, Deborah J. Frank, Kathryn G. Miller

Biology Faculty Research

During spermatid individualization in Drosophila, actin structures (cones) mediate cellular remodeling that separates the syncytial spermatids into individual cells. These actin cones are composed of two structural domains, a front meshwork and a rear region of parallel bundles. We show here that the two domains form separately in time, are regulated by different sets of actin-associated proteins, can be formed independently, and have different roles. Newly forming cones were composed only of bundles, whereas the meshwork formed later, coincident with the onset of cone movement. Polarized distributions of myosin VI, Arp2/3 complex, and the actin-bundling proteins, singed (fascin) and quail …

Supplementary Figure 1, Melissa J. Coleman, Arani Roy, J. Martin Wild, Richard Mooney

WM Keck Science Faculty Papers

Supplemental data for "Thalamic Gating of Auditory Responses in Telencephalic Song Control Nuclei."

Extracellular Stimuli Specifically Regulate Localized Levels Of Individual Neuronal Mrnas, Dianna E. Willis, Erna A. Van Niekerk, Yukio Sasaki, Mariano Mesngon, Tanuja T. Merianda, Gervan G. Williams, Marvin Kendall, Deanna S. Smith, Gary J. Bassell, Jeffery L. Twiss

Faculty Publications

Subcellular regulation of protein synthesis requires the correct localization of messenger RNAs (mRNAs) within the cell. In this study, we investigate whether the axonal localization of neuronal mRNAs is regulated by extracellular stimuli. By profiling axonal levels of 50 mRNAs detected in regenerating adult sensory axons, we show that neurotrophins can increase and decrease levels of axonal mRNAs. Neurotrophins (nerve growth factor, brainderived neurotrophic factor, and neurotrophin-3) regulate axonal mRNA levels and use distinct downstream signals to localize individual mRNAs. However, myelin-associated glycoprotein and semaphorin 3A regulate axonal levels of different mRNAs and elicit the opposite effect on axonal mRNA …

A Hybrid First Year Science Course For Engineering Students – Integrating Biology With Chemistry, David Harding, Pauline Schwartz, Jean Nocito-Gobel, Agamemnon Koutsospyros

Civil Engineering Faculty Publications

Biology is playing an increasingly important role in many engineering fields. With the typical engineering program already having a high credit hour requirement, the question becomes, how to best integrate biology concepts into a packed engineering curriculum. A typical biology course is not likely to introduce the important concepts of biology to engineering students. The solution here is to develop a hybrid course that integrates chemistry and biology. In the course, Chemistry with Applications to Biosystems, the concept is to develop a course that integrally links important concepts of chemistry and biology. The course focuses on the areas of biology …

Colon Cancer-- A Silent Killer, Katelyn Carman

Whittier Scholars Program

Colon cancer is the second leading cause of cancer deaths annually. The goal of this research is to (a) increase awareness of colon cancer by identifying anatomical and physiological changes that occur when cancerous polyps are present, (b) describe different genetic mutations that occur, (c) discuss preventative screening procedures and (d) explain different treatment options for colon cancer patients. There will be an additional section on the genetic link to colon cancer because it is thought to be the main cause of some cancerous polyps. Being aware of the potential risks associated with colon cancer can save lives.

Identification And Control Of Pest Slugs And Snails For Broadacre Crops In Western Australia, Svetlana Micic, Ken Henry, Paul Horne

Bulletins 4000 -

The numbers of slugs and snails have increased in broadacre cropping in Western Australia with the use of minimum tillage and stubble-retention practices. The organic content of paddocks increases under such systems, providing an increased food source especially to young slugs and snails. Soil moisture content is greater over summer leading to higher survival levels of slugs and snails.

Slug and snail pests in Australia have come from other countries, mainly the Mediterranean region. They damage plant seeds (mainly legumes), recently germinated seeds, seedlings and leaves and can be a contaminant of grain at harvest.

Search For A Knox Gene In Kalanchöe Pinnatum, Ryan Michael Gleason

Undergraduate Honors Thesis Collection

Knox genes have been found in nearly all eukaryotic organisms. These genes code for a protein that is able to bind DNA. By binding to DNA, it is capable of controlling the expression of other genes. The coded sequence of these genes has been determined in many different organisms, but has yet to be searched for in the air plant, Kalanchoe pinnatum. I did research over the past year in an attempt to find the coded sequence of Kalanchoe's Knox gene. I grew Kalanchoe in Butler's greenhouse to accumulate enough plant material to begin work. I then extracted the RNA …

Chemical Defenses: From Compounds To Communities, Valerie J. Paul, Koty H. Sharp

Arts & Sciences Faculty Publications

Marine natural products play critical roles in the chemical defense of many marine organisms and in some cases can influence the community structure of entire ecosystems. Although many marine natural products have been studied for biomedical activity, yielding important information about their biochemical effects and mechanisms of action, much less is known about ecological functions. The way in which marine consumers perceive chemical defenses can influence their health and survival and determine whether some natural products persist through a food chain. This article focuses on selected marine natural products, including okadaic acid, brevetoxins, lyngbyatoxin A, caulerpenyne, bryostatins, and isocyano terpenes, …

Increasing Serum 25-Hydroxyvitamin D As A Possible Deterrent To The Onset Of Multiple Sclerosis, Nicholas J. Perretti

Seton Hall University Dissertations and Theses (ETDs)

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The Role Of B2-Glycoprotein I In Homeostatis, Frederic Lin

Doctoral

B2-glycoprotein I (B2GPI) is a phospholipid-binding protein of 326 amino acids found in plasma at a concentration of approx. 180 ug/ml. It has a sequence of positively charged amino acids located at the carboxy terminus that mediates anionic phospholipid binding. B2GPI is suspected to have a role in inhibition of thrombosis. This suspicion is reinforced by the observation that B2GPI is the major target for autoantibodies in the antiphospholipid syndrome. However, little is known about circulating levels of the protein in common thrombotic diseases of inflammation. In the first part of this thesis, we developed a sensitive sandwich-direct ELISA for …

Linking Bacterioplankton Community Structures To Environmental State Variables And Phytoplankton Assemblages In Two South Carolina Salt Marsh Estuaries, Wes Johnson, Alan J. Lewitus, Madilyn Fletcher

Faculty Publications

Microorganisms are among the most important organisms to the ecology of salt marsh estuaries; however, fundamental questions regarding their distribution, environmental controls, and interactions with phytoplankton remain unanswered. We used denaturing gradient gel electrophoresis (DGGE) of bacterial rRNA genes and high performance liquid chromatography (HPLC) of phytoplankton photopigments to characterize planktonic communities from the Ashepoo, Combahee, and Edisto (ACE) Basin and North Inlet (NI) estuaries on the South Carolina coast, USA. Multivariate comparisons of the planktonic community profiles revealed that the 2 estuaries supported distinct bacterial communities. Furthermore, bacterial communities in both systems were partitioned into separate particle-associated (PA) and …

Chironomid Hemoglobin Genetic Diversity As An Indicator Of The New Jersey Hackensack Meadowlands Wetland Health, Lene Marie De Coursin Jacobs

Seton Hall University Dissertations and Theses (ETDs)

Kearny Marsh located within the Hackensack Meadowlands is a freshwater wetland impacted by multiple environmental stressors from human activity. The marsh is located in the northeastern New Jersey metropolitan area. Chironomid larvae, aquatic larvae of midge fly, were analyzed from two Kearny Marsh sample sites, W9 and W22. A previous sediment analysis and toxicity study characterized heavy metal contaminants the marsh. The data results were applied in this thesis study to determine the correlation between environmental stressors and chironomid genetic diversity. Site W9 sediment was impacted by severe levels of total heavy metal contamination, whereas site W22 had just below …

Ecological Fitting As A Determinant Of The Community Structure Of Platyhelminth Parasites Of Anurans, Daniel R. Brooks, Virginia León-Règagnon, Deborah Mclennan, Derek Zelmer

Faculty Publications

Host–parasite associations are assumed to be ecologically specialized, tightly coevolved systems driven by mutual modification in which host switching is a rare phenomenon. Ecological fitting, however, increases the probability of host switching, creating incongruences between host and parasite phylogenies, when (1) specialization on a particular host resource is a shared characteristic of distantly related parasites, and (2) the resource being tracked by the parasite is widespread among many host species. We investigated the effect of ecological fitting on structuring the platyhelminth communities of anurans from a temperate forest and grassland in the United States and tropical dry and wet forests …

In Vitro Expression And Purification Of Class I Mhc Molecules, Loi Cheng

Honors Scholar Theses

The major histocompatibility complex (MHC) is a gene family responsible for many critical functions of the immune system in most vertebrates. The MHC consists of three classes differentiated by their structure and function, and MHC class I encodes antigen binding proteins as well as chaperone and accessory proteins such as tapasin. The purpose of this project is to reconstitute several human MHC class I molecules in their peptide-filled and peptide-deficient forms, and to purify these proteins for biochemical study. The expressed proteins include wild type and mutant variants of the fusion protein human leukocyte antigen HLA-B*0801-fos, and human beta-2-microglobulin (β2m). …

Closing The Loop: Controlling Type 1 Diabetes Mellitus, Megan Chynoweth

Whittier Scholars Program

Diabetes mellitus is an ever increasing pandemic. Type 1 diabetes mellitus (T1DM), although not the most prevalent form of diabetes mellitus, has had an “average annual increase of 3.09%, meaning the incidence of T1DM would increase 40% from 1998 to 2010”. T1DM leads to many complications: adverse side effects such as retinopathy, neuropathy, and nephropathy. These comorbidities are costing the United States billions of healthcare dollars per year. Delaying complications result in cost savings and improved quality of life, which can be obtained by tightly controlling blood sugars. However, strict control is difficult to attain. Optimal glucose levels may be …

Biolojam – The Educational Comic, Stacey Barrie

Senior Honors Projects

BioloJam is a biology comic aimed towards 7th and 8th grade students. The goal of the project was to create an educational resource that students would find entertaining and enlightening. The project is available on the web and is free to use for educational purposes by teachers, students, and parents. There are two comics: the larger, more complicated “Cell Breakout” and the smaller, more conversational “Giraffic Park.” In Cell Breakout, the three heroes are trapped inside a video game in a plant cell, and learn all about cell functions as they try to escape. In Giraffic Park, the trio are …

Dolomedes Scriptus (Water Spider): Male Response To Females’ Putative Pheromones On Perches., Katherine Hunt

Undergraduate Theses and Capstone Projects

Sexual signals are commonly used among animals to enhance reproductive success. Chemical signals such as pheromones are commonly used to attract mates. Little is known, though, about how these signals are received or interpreted by the target. I studied the water spider, Dolomedes scriptus in the pisaurid family. Most Dolomedes species only make webs as a nursery for their young. However, females often create silk drag lines, potentially serving for species recognition. In some Dolomedes species, females who are previously mated are known to cannibalize most approaching males. Males might avoid cannibalism by avoiding mated females, particularly if they can …

Plasmid Probes For Screening Specific Genes In Large Fragment Dna Libraries, Leah Ferguson

Senior Honors Projects

Heliothis virescens, a member of the lepidopteran family, is of great importance to many people who rely on agriculture as a source of income because it is a pest of many important crops. Because H. virescens feeds on a broad array of hosts including cotton, tobacco, tomato, and soybean it has earned its place as a major nuisance in the United States. Heliothis subflexa is a close taxonomic relative of H. virescens, but the two species differ in that H. subflexa is a specialized herbivore that only feeds on plants in the genus Physalis. Studies have been performed to determine …

Regulation Of Cytoplasmic Dynein Atpase By Lis1, Mariano T. Mesngon, Cataldo Tarricone, Sachin Hebbar, Aimee Guillotte, E. William Schmitt, Lorene Lanier, Andrea Musacchio, Stephen J. King, Deanna S. Smith

Faculty Publications

Mutations in Lis1 cause classical lissencephaly, a developmental brain abnormality characterized by defects in neuronal positioning.Over the last decade, a clear link has been forged between Lis1 and the microtubule motor cytoplasmic dynein. Substantial evidenceindicates that Lis1 functions in a highly conserved pathway with dynein to regulate neuronal migration and other motile events. Yeasttwo-hybrid studies predict that Lis1 binds directly to dynein heavy chains (Sasaki et al., 2000; Tai et al., 2002), but the mechanistic significance of this interaction is not well understood. We now report that recombinant Lis1 binds to native brain dynein and significantly increases the microtubule-stimulated enzymatic …

Establishment Of Polarity During Organization Of The Acentrosomal Plant Cortical Microtubule Array, Ram Dixit, Eric Chang, Richard Cyr

Biology Faculty Research

The plant cortical microtubule array is a unique acentrosomal array that is essential for plant morphogenesis. To understand how this array is organized, we exploited the microtubule (+)-end tracking activity of two Arabidopsis EB1 proteins in combination with FRAP (fluorescence recovery after photobleaching) experiments of GFP-tubulin to examine the relationship between cortical microtubule array organization and polarity. Significantly, our observations show that the majority of cortical microtubules in ordered arrays, within a particular cell, face the same direction in both Arabidopsis plants and cultured tobacco cells. We determined that this polar microtubule coalignment is at least partially due to a …

Photochemical Transformations Of Riverine Dissolved Organic Matter: Effects On Estuarine Bacterial Metabolism And Nutrient Demand, Erik M. Smith, Ronald Benner

Faculty Publications

By experimentally simulating the exposure of terrigenous dissolved organic matter (DOM) to sunlight during the mixing of river water and seawater in an estuary (St. Helena Sound, South Carolina), this study examined how photochemical alterations may affect both the magnitude and fate of DOM utilization by estuarine bacteria. In a series of experiments performed with water samples collected from 3 blackwater rivers draining into an estuary of the South Atlantic Bight (USA), exposure to light resulted in significant decreases in dissolved organic carbon (DOC) concentration and DOM absorption at 350 nm relative to dark controls. When these water samples were …

Phosphotyrosine Phosphatases Of The Att-20 Murine Corticotroph Cell Line, Kari A. Belin

Seton Hall University Dissertations and Theses (ETDs)

Somatostatin (somatotropin release inhibitory factor; SRIF) is a peptide-signaling molecule that activates a family of heterotrimeric guanine nucleotide binding protein (G­ protein) coupled receptors (sst.-sst-). SRIF receptors control essential intracellular signaling events and, by reducing cyclic nucleotide levels, ion concentrations and protein phosphorylation, ultimately controlling cell proliferation and secretion. In the current study, we investigated the intracellular phosphatase activity present in the AtT-20 cell, as well as whether these enzymes were under direct SRIF receptor control. AtT-20 cells retain many of the properties of anterior pituitary corticotrophs, yet are an established cell line that expresses at least two SRIF receptor …

Law And Behavioral Biology, Owen D. Jones, Timothy H. Goldsmith

Vanderbilt Law School Faculty Publications

Society uses law to encourage people to behave differently than they would behave in the absence of law. This fundamental purpose makes law highly dependent on sound understandings of the multiple causes of human behavior. The better those understandings, the better law can achieve social goals with legal tools. In this Article, Professors Jones and Goldsmith argue that many long held understandings about where behavior comes from are rapidly obsolescing as a consequence of developments in the various fields constituting behavioral biology. By helping to refine law's understandings of behavior's causes, they argue, behavioral biology can help to improve law's …

Size And Organic Content Of Eggs Of Marine Annelids, And The Underestimation Of Egg Energy Content By Dichromate Oxidation, William Jaeckle, Bruno Pernet

Scholarship

Dichromate oxidation is a simple technique that is often used to estimate the energy content of eggs in studies of marine invertebrate life histories (1). We used this method to measure the energy contents of the eggs of 12 species of marine annelids. In combination with measures of egg ash-free dry weight (AFDW), these data yielded estimates of AFDW-specific energy density that were mostly lower than the average weight-specific energy density of carbohydrates. This seemed unlikely to be correct, as invertebrate eggs typically contain little carbohydrate and instead are composed primarily of energy-dense protein and lipid (1, 2). After validating …

A Patch Hath Smaller Patches: Delineating Ecological Neighborhoods For Parasites, Derek Zelmer, John R. Seed

Faculty Publications

Use of the host individual as a boundary for parasite populations and communities provides an unambiguous spatial unit that is useful for pattern description, but this framework precludes consideration of the host landscape and within-host population dynamics. Recognizing host individuals as spatially and temporally complex landscapes requires modified concepts of parasite populations and communities. An outline of the currently accepted hierarchies of parasite populations and communities is provided on the basis of ecological neighborhoods that are delineated by discrete habitat patches or functional dynamics (or both), as opposed to host individuals. This parasite-based framework accommodates consideration of both within- and …