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2025

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Articles 871 - 900 of 1658

Full-Text Articles in Biology

Analyzing Microalgae-Bacteria Population Changes In Response To Environmental Parameters For Improved Wastewater Treatment, Amanda M. Moravek May 2025

Analyzing Microalgae-Bacteria Population Changes In Response To Environmental Parameters For Improved Wastewater Treatment, Amanda M. Moravek

All Graduate Theses and Dissertations, Fall 2023 to Present

Microalgae are incredible organisms that have a variety of uses such as wastewater treatment, fertilizer, livestock feed, and bio-compound production. Microalgae require a light source and minimal nutrients space to proliferate, making them an environmentally friendly and sustainable option for such processes. The Sustainable Wastes-to-Bioproducts Engineering Center at Utah State University is developing and implementing a Rotating Algae Biofilm Reactor (RABR) to remove total nitrogen and phosphorous from the Central Valley Wastewater Reclamation Facility wastewater effluent. Harmful compounds such as nitrates and phosphates are metabolized by the microalgae and converted into useful bioproducts, resulting in a circular economy. These RABRs …


Seasonal And Temporal Influences On Successful Crossing Rates By Ungulates Of A North Utah Wildlife Overpass, Blake Ledbetter May 2025

Seasonal And Temporal Influences On Successful Crossing Rates By Ungulates Of A North Utah Wildlife Overpass, Blake Ledbetter

All Graduate Theses and Dissertations, Fall 2023 to Present

Encroachment of human infrastructure fragments wild landscapes and introduces a suite of negative impacts for humans and wildlife. In Utah, roadways threaten migratory ungulates by creating a barrier to movements and preventing access to seasonal migration routes. To mitigate these conflicts, state transportation agencies often install wildlife crossing structures to allow animals to safely cross over or under roadways. Selecting the proper crossing structure requires consideration of the species of interest, landscape, location, and structural dimensions. Often there is balance between the optimal location, the dimensions of the crossing structures, and what is feasible given the landscape. In 2018, the …


Uv-A Light And Heat-Activated Antimicrobial And Biodegradable Materials For Food Packaging And Preservation, Michael J. Bodily May 2025

Uv-A Light And Heat-Activated Antimicrobial And Biodegradable Materials For Food Packaging And Preservation, Michael J. Bodily

All Graduate Theses and Dissertations, Fall 2023 to Present

Current food packaging materials are often non-biodegradable and harmful for ingestion, which poses a threat to the environment and public health. Thus far, the dangers of microbial contamination of food on human health have outweighed the risks associated with using these conventional packaging plastics. A possible solution to these issues is to use a combination of innovative materials and microbial inactivation methods that can potentially both reduce plastic waste and combat harmful microorganisms. In this study, two antimicrobial, biodegradable composites were developed and tested for their ability to inactivate two types of bacterium, Escherichia coli K12 and Listeria innocua, …


Melanaphis Sacchari/Sorghi Complex: Current Status, Challenges And Integrated Strategies For Managing The Invasive Sap-Feeding Insect Pest Of Sorghum, Alejandro Vasquez, Joseph Belsky, Neetu Khanal, Heena Puri, Devi Balakrishnan, Neelendra K. Joshi, Joe Louis, Glenn Studebaker, Rupesh Kariyat May 2025

Melanaphis Sacchari/Sorghi Complex: Current Status, Challenges And Integrated Strategies For Managing The Invasive Sap-Feeding Insect Pest Of Sorghum, Alejandro Vasquez, Joseph Belsky, Neetu Khanal, Heena Puri, Devi Balakrishnan, Neelendra K. Joshi, Joe Louis, Glenn Studebaker, Rupesh Kariyat

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Melanaphis sacchari (Zehntner;Hemiptera: Aphididae), sugarcane aphid (SCA), is an invasive phloem-feeder found worldwide with a wide host range of economically important plants including sorghum and sugarcane. Given its high reproductive capacity and ability to rapidly spread over long distances, SCA presents challenges for effective control, leading to substantial economic losses. Recent studies have identified two multiloci SCA genotypes specialized in feeding on sugarcane (MLL-D) and sorghum (MLL-F) in the USA, which raises concerns as the USA is the second largest sorghum-producing country. This has encouraged research towards identifying these two biotypes where some research has stated them as two species; …


Advancement Of Wrongful Conviction Prevention Strategies In Forensic Science, Isabella Pinto May 2025

Advancement Of Wrongful Conviction Prevention Strategies In Forensic Science, Isabella Pinto

Honors Projects

The National Registry of Exonerations (NRE) has identified 1,051 of its logged cases to involve forensic evidence in some way, nearly one-third of the total 3,600 exonerations in its database. Analysis of the NRE and Innocence Project databases revealed the most common factors in forensic-related wrongful convictions: expert testimony, flawed methodologies (most of which are no longer in use), and statistical misinterpretations of results. The majority of scholars agree that oversight in forensic casework must be improved in order to meaningfully resolve issues. Creation of commissions in each state would address all of the identified errors, though not a catch-all …


Intraspecific Genetic Variation In Green Salamanders (Aneides Aeneus) At Bays Mountain Park, Brianna Drake May 2025

Intraspecific Genetic Variation In Green Salamanders (Aneides Aeneus) At Bays Mountain Park, Brianna Drake

Electronic Theses and Dissertations

Accurate taxonomy is crucial for conserving vulnerable cryptic species like the green salamander (Aneides aeneus) in the Southern Appalachian Mountains. Prior studies identified three distinct lineages within the A. aeneus complex: Northern Appalachians, Southern Appalachians, and Blue-Ridge Escarpment. One previously collected sample indicated green salamanders in northeast Tennessee fit within the Northern lineage. The present study uses molecular data of A. aeneus at Bays Mountain Park (BMP) in Kingsport, Tennessee, to determine where the BMP population fits within these lineages. It was hypothesized that individuals from BMP belong in the northern lineage. Tail tips were collected from thirty-four …


Pastoral Land Condition: Kimberley Land Condition Standards, Robert Sudmeyer, Rick Fletcher, Kathryn Ryan, Matthew Fletcher, Charles Martin, Philip Thomas, Karen Holmes, Karyn Reeves, Wayne Fletcher May 2025

Pastoral Land Condition: Kimberley Land Condition Standards, Robert Sudmeyer, Rick Fletcher, Kathryn Ryan, Matthew Fletcher, Charles Martin, Philip Thomas, Karen Holmes, Karyn Reeves, Wayne Fletcher

Natural resources published reports

This report outlines the conceptual basis and quantitative land condition standards that define acceptable pastoral land condition for the Kimberley, Western Australia (WA), to meet the regulatory requirements of the Land Administration Act 1997 and the Soil and Land Conservation Act 1945.

The Department of Primary Industries and Regional Development (DPIRD) develops regional-level pastoral land condition standards for WA to implement the Framework for sustainable pastoral management – Revised edition, which is designed to address the Office of the Auditor General’s recommendations to improve the management of pastoral lands in WA. The application of these standards is expected to …


Development Of A Behavioral Paradigm To Monitor Seizure Susceptibility And Severity In A Zebrafish Model Of Cblx Syndrome, Claudia Berenice Gil May 2025

Development Of A Behavioral Paradigm To Monitor Seizure Susceptibility And Severity In A Zebrafish Model Of Cblx Syndrome, Claudia Berenice Gil

Open Access Theses & Dissertations

Methylmalonic acidemia and homocysteinemia cblX type (cblX) (MIM#309541) is a rare, X-linked recessive disorder caused by a mutation in the HCFC1 gene. This disorder is characterized by multiple congenital anomalies which include intellectual disability, brain malformations, intractable epilepsy, microcephaly, and facial dysmorphia. The most severe symptom of cblX is intractable epilepsy which is 100% penetrant. Our lab developed a zebrafish model with a mutation in the zebrafish hcfc1a ortholog to study the mechanisms underlying seizure phenotypes in cblX. Our laboratory previously showed that mutation of hcfc1a results in increased number and proliferation of neural precursor cells (NPCs) and an increase …


Assessing The Osmoregulatory Capabilities Of American Alligator Lingual Glands, Benjamin Angalet, Nicholas S. Green Apr 2025

Assessing The Osmoregulatory Capabilities Of American Alligator Lingual Glands, Benjamin Angalet, Nicholas S. Green

Symposium of Student Scholars

Crocodilians inhabit a wide variety of aquatic, marine, and estuarine habitats on 4 continents. Saltwater-adapted species and populations face considerable osmoregulatory challenges in maintaining homeostasis with respect to plasma salt [NaCl] concentration. In addition to the kidneys and cloaca found in all crocodilians, crocodiles possess a salt-secreting lingual gland whose presence and function is less understood in other crocodilians. This study investigates the lingual gland anatomy, physiology, and potential function of American alligator (Alligator mississippiensis) populations exposed to varying salinity levels. At each of four sites (Jekyll Island, Sapelo Island, Okefenokee NWR, and Banks Lake NWR), we will capture 12 …


Predicting Healthcare Service Quality Based On A Kalman-Optimized Bi-Lstm-Inspired Deep Learning Model, Mohammed K. Al-Khafaji, Eman S. Al-Shamery Apr 2025

Predicting Healthcare Service Quality Based On A Kalman-Optimized Bi-Lstm-Inspired Deep Learning Model, Mohammed K. Al-Khafaji, Eman S. Al-Shamery

Karbala International Journal of Modern Science

Health is one of the most important aspects of human well-being, and access to high-quality healthcare is essential for a good quality of life. Providing top-level health services at all times is crucial. However, the research in healthcare poses significant challenges due to the diversity and variations of medical practices across different hospitals. This paper aims to tackle the challenge of data missing and scattering during data collection. Then, the quality of services (QoS) offered by healthcare facilities will be analyzed and predicted from the patient's perspective. The model begins preprocessing data by data cleaning, handling missing values, and scattering …


Gonadal Absence Of Ceh-22: Degradation Of Ceh-22 In The Gonads Of Caenorhabditis Elegans, Andreya C. White Apr 2025

Gonadal Absence Of Ceh-22: Degradation Of Ceh-22 In The Gonads Of Caenorhabditis Elegans, Andreya C. White

Undergraduate Theses

The reproductive system of hermaphrodite Caenorhabditis elegans (C. elegans) comprises two gonadal arms, each with a terminating distal tip cell (DTC), connected by a common uterus. The DTC has two functions: leader and niche. In C. elegans, ceh-22/nkx2,5 has been found to work together with Wnt signaling to determine the fates of the DTC (Lam et al., 2006). This is an indication that ceh-22 aids in the specification of DTCs. Our research aims to determine the specific function of ceh-22 in the gonad of both male and hermaphrodite C. elegans. This will be accomplished by observing …


Plant Diversity Dynamics Over Space And Time In A Warming Arctic, Mariana García Criado, Isla H. Myers-Smith, Anne D. Bjorkman, Sarah C. Elmendorf, Signe Normand, Peter Aastrup, Rien Aerts, Juha M. Alatalo, Lander Baeten, Robert G. Björk, Mats P. Björkman, Noémie Boulanger‐Lapointe, Ethan E. Butler, Elisabeth J. Cooper, J. Hans C. Cornelissen, Gergana N. Daskalova, Belen Fadrique, Bruce C. Forbes, Greg H. R. Henry, Robert D. Hollister, Toke Thomas Høye, Ida Bomholt Dyrholm Jacobsen, Annika K. Jägerbrand, Ingibjörg S. Jónsdóttir, Elina Kaarlejärvi, Olga Khitun, Kari Klanderud, Tiina H. M. Kolari, Simone I. Lang, Nicolas Lecomte, Jonathan Lenoir, Petr Macek, Julie Messier, Anders Michelsen, Ulf Molau, Robert Muscarella, Marie-Louise Nielsen, Matteo Petit Bon, Eric Post, Katrine Raundrup, Riikka Rinnan, Christian Rixen, Ingvild Ryde, Josep M. Serra‐Diaz, Gabriela Schaepman‐Strub, Niels M. Schmidt, Franziska Schrodt, Sofie Sjögersten, Manuel J. Steinbauer, Lærke Stewart, Beate Strandberg, Anne Tolvanen, Craig E. Tweedie, Mark Vellend Apr 2025

Plant Diversity Dynamics Over Space And Time In A Warming Arctic, Mariana García Criado, Isla H. Myers-Smith, Anne D. Bjorkman, Sarah C. Elmendorf, Signe Normand, Peter Aastrup, Rien Aerts, Juha M. Alatalo, Lander Baeten, Robert G. Björk, Mats P. Björkman, Noémie Boulanger‐Lapointe, Ethan E. Butler, Elisabeth J. Cooper, J. Hans C. Cornelissen, Gergana N. Daskalova, Belen Fadrique, Bruce C. Forbes, Greg H. R. Henry, Robert D. Hollister, Toke Thomas Høye, Ida Bomholt Dyrholm Jacobsen, Annika K. Jägerbrand, Ingibjörg S. Jónsdóttir, Elina Kaarlejärvi, Olga Khitun, Kari Klanderud, Tiina H. M. Kolari, Simone I. Lang, Nicolas Lecomte, Jonathan Lenoir, Petr Macek, Julie Messier, Anders Michelsen, Ulf Molau, Robert Muscarella, Marie-Louise Nielsen, Matteo Petit Bon, Eric Post, Katrine Raundrup, Riikka Rinnan, Christian Rixen, Ingvild Ryde, Josep M. Serra‐Diaz, Gabriela Schaepman‐Strub, Niels M. Schmidt, Franziska Schrodt, Sofie Sjögersten, Manuel J. Steinbauer, Lærke Stewart, Beate Strandberg, Anne Tolvanen, Craig E. Tweedie, Mark Vellend

Wildland Resources Faculty Publications

The Arctic is warming four times faster than the global average1 and plant communities are responding through shifts in species abundance, composition and distribution2,3,4. However, the direction and magnitude of local changes in plant diversity in the Arctic have not been quantified. Using a compilation of 42,234 records of 490 vascular plant species from 2,174 plots across the Arctic, here we quantified temporal changes in species richness and composition through repeat surveys between 1981 and 2022. We also identified the geographical, climatic and biotic drivers behind these changes. We found greater species richness …


Bibliometric Analysis Of Research Trends Related To Human Immunodeficiency Virus., Madyson E. Johnson, Kaitlyn R. Coffman, Valerie Rivas, Elizabeth Barrett Apr 2025

Bibliometric Analysis Of Research Trends Related To Human Immunodeficiency Virus., Madyson E. Johnson, Kaitlyn R. Coffman, Valerie Rivas, Elizabeth Barrett

ATU Scholars Symposium

Human immunodeficiency virus poses major health burden across the globe including the United States of America and has its zoonotic origin from the Simian Immunodeficiency viruses of sooty mangabey monkey and chimpanzee in Africa. Epidemiological trends indicate that even though the mortality rates for HIV are falling globally, the rates of new cases have been rising since 2010 in several countries including the USA. An estimated 0.5% of the human population is currently infected with the virus with countries like USA, India, Nigeria and South Africa carrying the highest burden of the disease. Host receptors such as CD4 and coreceptors …


Discovering And Characterizing Novel Temperate Bacteriophage Infecting Arthrobacter Globiformis B-2979, Paige M. Baldwin, Michael Bennet, Evangelia A. Buonamici, Hanan E. Isik, Niran E. Isik, Vienna Li, Mia R. Mazakas, Aaryan M. Modi, Nikita Muppoor, Kylie A. Oliver, Vidhi M. Patel, Kylie Tabak, Jamie K. Yu, Laura Weber Apr 2025

Discovering And Characterizing Novel Temperate Bacteriophage Infecting Arthrobacter Globiformis B-2979, Paige M. Baldwin, Michael Bennet, Evangelia A. Buonamici, Hanan E. Isik, Niran E. Isik, Vienna Li, Mia R. Mazakas, Aaryan M. Modi, Nikita Muppoor, Kylie A. Oliver, Vidhi M. Patel, Kylie Tabak, Jamie K. Yu, Laura Weber

STEM Student Research Symposium Posters

AmiCi24 is a novel temperate bacteriophage collected from a soil sample originating in Sewell, NJ, USA and cultivated with Arthrobacter globiformis B-2979. AmiCi24 is a Siphoviridae morphotype, belonging to the cluster AS and subcluster of AS3. The AmiCi24 genome consists of 38,466 basepairs, encoding 68 genes.


Cellular And Molecular Mechanism Of Heavy Metal Phytoremediation, Kaitlin A. Edwards, Kayle Dacus, Autumn Wilbanks, Tatum Simms Apr 2025

Cellular And Molecular Mechanism Of Heavy Metal Phytoremediation, Kaitlin A. Edwards, Kayle Dacus, Autumn Wilbanks, Tatum Simms

ATU Scholars Symposium

Rapid urbanization and industrial activities have contributed to heavy metal contamination of several habitats thus leading to an emerging health and environmental challenge. Some heavy metals such as zinc, iron, molybdenum, manganese, copper, cobalt and nickel are found in trace amounts in biological organisms, however, most are harmful for cellular metabolism. Certain species of plants can accumulate large amounts of heavy metals and has been utilized to sustainably clean contaminated soil and water by a process called phytoremediation. Recent lab and field studies have reported promising results that could propel commercialization of this technology. The cellular and molecular mechanism involved …


Comparing Parasitism And Resistance To Flow In Two Populations Of Elimia Virginica, Morgan G. Hicok, Abbie Tomba, Kate Green Apr 2025

Comparing Parasitism And Resistance To Flow In Two Populations Of Elimia Virginica, Morgan G. Hicok, Abbie Tomba, Kate Green

Departmental Honors & Graduate Capstone Projects

Snails are important in freshwater ecosystems as primary consumers and are good indicators of the stability of a habitat. Trematodes are parasitic flatworms that infect freshwater snails and can cause changes in metabolic function and growth. Flow rates and water levels are now more variable due to climate change, increasing stress and dislodgment in the snail population. This study investigates how parasites, shell morphology, and native flow environment influence resistance to disturbance. Elimia populations were sampled for differences in parasitism, shell morphology, and ability to withstand high flow environments in two different rivers. Sample sites at the Potomac River were …


Factors That Contribute To An Increased Risk For Dental Caries And Prevention Methods, Allen Lee Apr 2025

Factors That Contribute To An Increased Risk For Dental Caries And Prevention Methods, Allen Lee

BIO4960: Practicum in Biology I

This health literacy project focuses on identifying effective strategies for preventing dental caries by analyzing various aspects of an individual’s daily habits. Key lifestyle factors such as sleep patterns, dietary choices, and physical activity were explored in relation to their impact on oral health. The motivation for this project arose from direct observations of the high prevalence of cavities during clinical shadowing experiences, as well as growing evidence in the literature, highlighting an increasing trend in dental caries. Dental caries represent significant public health concern, as untreated caries can lead to serious complications such as tooth abscesses and extractions. Despite …


Quantifying And Characterizing Microplastics In The Greater Philadelphia Region, Emily Lybashev Apr 2025

Quantifying And Characterizing Microplastics In The Greater Philadelphia Region, Emily Lybashev

Biology Honors Papers

Plastic is one of the world's most useful and versatile materials, so it is no wonder that 400 million tons are produced yearly. When exposed to weathering, heat, water, and organisms, plastic degrades and produces microplastics. These microplastics have managed to leach into our oceans, local waterways, bodies, and even our atmosphere. The negative implications of microplastic contamination in our environment are slowly being uncovered with concerns of toxin buildup. Additionally, the amount of microplastics we are exposed to through local waterways is rarely quantified. Though microplastic research is picking up pace, the Schuylkill River which spans the Greater Philadelphia …


The Role And Expression Of Pros-1 In The C. Elegans Gonad, Brandon W. Thomas, Mary B. Kroetz, Roberta Challener Apr 2025

The Role And Expression Of Pros-1 In The C. Elegans Gonad, Brandon W. Thomas, Mary B. Kroetz, Roberta Challener

Undergraduate Theses

The nematode, formally known as Caenorhabditis elegans, has served as an excellent model organism in understanding fundamental biological processes, including those governed by conserved genes like pros-1. pros-1 is partially responsible for the formation of tubular structures within the organism. Glial cells and excretory canals are both examples of tubular structures governed by pros-1. pros-1 orchestrates the embryonic development of these cell types by modulating cell proliferation and differentiation, thus ensuring proper morphogenesis and organogenesis. Its regulatory influence likely extends beyond embryogenesis, potentially affecting reproductive processes as well. Furthermore, the evolutionary conservation of pros-1 underscores its significance …


The Chemistry Of Decomposition: The Mechanisms To Decay The Human Body, Lydia J. Buxa Apr 2025

The Chemistry Of Decomposition: The Mechanisms To Decay The Human Body, Lydia J. Buxa

Senior Honors Theses

Taphonomic analysis separates decomposition into four major stages: fresh, early decomposition, advanced decomposition, and skeletonization. It is easy to demarcate the observable changes to the body, but it is far more difficult to know what underlying biochemical processes are occurring. Fresh decomposition begins with algor mortis, livor mortis, and rigor mortis, which sets the stage for the major workforces of decomposition: autolysis and putrefaction. The external signs of decomposition appear in early decomposition, which can be attributed to the breakdown of proteins, lipids, carbohydrates, and nucleic acids. This also leads to major changes in the moisture levels during advanced decomposition, …


How Biophilic Design Impacts Office Spaces, Addison Carter Apr 2025

How Biophilic Design Impacts Office Spaces, Addison Carter

Honors Projects

Many working individuals experience burnout and dissatisfaction in their jobs. Although is this a result of individual mental strain, or are there other environmental factors contributing to the problem? This study investigates the impact of design on well-being and examines how biophilic design can serve as a solution to the negative effects certain architectural features may have on occupants. A literature review was conducted; it analyzed existing research on topics such as office design, biophilic design, and the influence of architecture on comfort and wellness. To explore how biophilic design can be applied in all settings, not just new construction, …


Effects Of Roadways On Seasonal Movement Strategies And Mate Location Success In An Imperiled Pit Viper (Crotalus Horridus), Dominic Desantis Apr 2025

Effects Of Roadways On Seasonal Movement Strategies And Mate Location Success In An Imperiled Pit Viper (Crotalus Horridus), Dominic Desantis

Academic Affairs

A detailed understanding of animal movement behavior is fundamental to effective conservation and management. Within populations, a diversity of movement strategies can be displayed in search of critical resources, and these strategies are influenced by multiple interacting factors related to individuals and the environment. Mating partners are one critical resource that often serves as a prominent selective force shaping movement during mating seasons. For many large-bodied snakes, such as pit vipers (Viperidae: Crotalinae), male mate-searching movements are the primary determinant of mate location success. During this time, males incur significant risks associated with elevated movement. In an increasingly human-modified world, …


Ecology Of Beech Leaf Disease: Identifying The Forest Conditions Correlated With Infection In Northeast Ohio, Blake Logan Apr 2025

Ecology Of Beech Leaf Disease: Identifying The Forest Conditions Correlated With Infection In Northeast Ohio, Blake Logan

Research Symposium

Beech Leaf Disease (BLD) is a novel, poorly-understood threat to forests across North America. Since its discovery in northeast Ohio in 2012, the disease has affected staggering numbers of beech trees (Fagus grandifolia), resulting in increased sapling mortality. In the near future, mortality in symptomatic mature trees is expected to follow. The symptomatic striped leaves result from the activity of the nematode Litylenchus crenatae ssp. mccannii, but the precise role this organism plays in transmission and infection is currently unknown.

Oberlin College has been conducting forest demography research at Chance Creek, a nature preserve in Lorain county, since 1974. Every …


Global Health Concentration Journey, Leann Torrez Apr 2025

Global Health Concentration Journey, Leann Torrez

Research Symposium

The Oberlin College Global Health Concentration has played a crucial role in helping me strengthen my personal goals and professional vision, especially regarding the future I hope to shape for others. Before attending Oberlin, I knew I wanted to pursue a career in nursing, driven by both positive and negative experiences that my family, my communities, and I have encountered within the healthcare system. These experiences motivated me to acquire the necessary knowledge and skills to create a more equitable, accessible, and intentional healthcare environment for underrepresented communities. Throughout my time at Oberlin, I’ve been learning to think critically about …


The Biomedical Ethics Of Palliative Psychiatry In Anorexia Nervosa Patients, Mikayla Decesare Apr 2025

The Biomedical Ethics Of Palliative Psychiatry In Anorexia Nervosa Patients, Mikayla Decesare

Fisher Showcase 2025

My presentation is going to be created from the extensive research paper that I am writing on the biomedical ethics of palliative psychiatry in patients specifically with Anorexia Nervosa. The paper as well as the presentation will explore ethical frameworks that can be applied to this medical practice by diving into case studies, looking at arguments of people currently in the field, analyzing what criteria needs to be taken into account, and more. This Keystone was added to the Biomedical Ethics course I am taking this semester so the audience is my classmates. However, this presentation is also tailored to …


Progression Of Electrophysiological Impairments In Diabetic Cardiomyopathy And Intervention Using Enicostemma Axillare, Victor Arokia Doss, Dharaniyambigai Kuberapandian Apr 2025

Progression Of Electrophysiological Impairments In Diabetic Cardiomyopathy And Intervention Using Enicostemma Axillare, Victor Arokia Doss, Dharaniyambigai Kuberapandian

Turkish Journal of Biology

Earlier studies widely reported advanced ventricular impairments only at the later stages of diabetic cardiomyopathy (DCM). The present study aimed to understand the duration-based early to late stages of cardiac electrophysiological impairments using electrocardiography (ECG) and intervention using Enicostema axillare subsp. littorale. The experimental rats were streptozotocin (SZ)-induced diabetic rats that were administered E. axillare formulations as follows: (1) normal; (2) SZ - 40 mg/kg, single, i.p.); (3) SZ + insulin (2 U/day) + losartan (10 mg/kg); (4) SZ + insulin + losartan + E. axillare decoction (2 mL/day); (5) SZ + E. axillare decoction; (6) SZ + E. axillare …


A Tetracycline-Inducible Split Turboid System For Specific Biotinylation And Identification Of Nuclear Proteins From Hek293t Cells, Mehmet Sarihan, Fatma Zehra Özen, Murat Kasap, Gürler Akpinar Apr 2025

A Tetracycline-Inducible Split Turboid System For Specific Biotinylation And Identification Of Nuclear Proteins From Hek293t Cells, Mehmet Sarihan, Fatma Zehra Özen, Murat Kasap, Gürler Akpinar

Turkish Journal of Biology

Background/aim: To overcome the limitations of conventional organelle isolation methods including low purity, low yield, sample degradation, scalability and the need for multiple centrifugation steps, an improved nuclear protein enrichment approach was developed using the modified Split TurboID biotin ligase enzyme.

Materials and methods: A construct was created in which the N-terminal domain of TurboID, fused to the FK506-binding protein (FKBP) was targeted to the nucleus. This construct was incorporated into a tetracycline-inducible gene expression vector. Similarly, the C-terminal domain of TurboID was fused to the rapamycin-binding domain of mTOR (FRB) and directed to the nucleus. This construct …


All Stressed Out: An Investigation On The Effects Of Environmental Stressors On The Northern Leopard Frog (Rana Pipiens), Ella C. Wittmuss Apr 2025

All Stressed Out: An Investigation On The Effects Of Environmental Stressors On The Northern Leopard Frog (Rana Pipiens), Ella C. Wittmuss

Honors Thesis

Corticosterone (CORT) is the primary glucocorticoid in many vertebrates, including mammals, birds, reptiles, and amphibians. When an organism is exposed to external or internal stressors, CORT levels often increase (Rich & Romero, 2005; Titon et al., 2021). An increase in CORT can cause adverse physiological effects such as slower growth, malformations in different parts of the body, and specific blood sugar diseases such as diabetes (Glennemeier & Denver, 2002; Tarlow &Blumstein, 2007). CORT levels are indicators of survival, growth, and reproduction in larval amphibians. Many species, including our model organism, the Northern Leopard Frog (Rana pipiens), are often subjected to …


Apigenin Attenuates Ischemia–Reperfusion-Induced Pulmonary Ferroptosis And Fibrosis By Activating The Nrf2/Ho-1/Gpx4 Axis In Mice, Liang Zhang, Haojie Li, Shichen Xu, Hao Wen, Chaoxiao Yu Apr 2025

Apigenin Attenuates Ischemia–Reperfusion-Induced Pulmonary Ferroptosis And Fibrosis By Activating The Nrf2/Ho-1/Gpx4 Axis In Mice, Liang Zhang, Haojie Li, Shichen Xu, Hao Wen, Chaoxiao Yu

Turkish Journal of Biology

Background/aim: Acute lung injury (ALI) is a major cause of morbidity and mortality after lung ischemia-reperfusion injury (LIRI). In recent years, pulmonary ferroptosis and its associated fibrosis have been recognized as important causes of LIRI. The purpose of this study is to investigate apigenin (APG) as a potential therapeutic target for treating LIRI-induced pulmonary ferroptosis and fibrosis.

Materials and methods: A rat model of LIRI was established and the rats were randomly divided into three groups, a sham group, a LIRI group, and an APG group. The pathological changes of the lung tissue were evaluated using hematoxylin-eosin staining and …


Prooxidant Effect Of Uric Acid On Human Leukocytic Dna: An In Vitro And Ex Vivo Study, Yim Tong Savio Szeto, Vincy Sze Wing Li, Yuen Lam Pon Apr 2025

Prooxidant Effect Of Uric Acid On Human Leukocytic Dna: An In Vitro And Ex Vivo Study, Yim Tong Savio Szeto, Vincy Sze Wing Li, Yuen Lam Pon

Turkish Journal of Biology

Background/aim: Uric acid is a major contributor to the total antioxidant capacity of human plasma. However, this endogenous substance’s antioxidant and prooxidant properties have not yet been reported.

Conclusion: In this study, the comet assay was employed in vitro to determine the effect of uric acid on DNA damage in human lymphocytes and leukocytic DNA damage in hyperuricemia patients with and without renal failure.

Results: DNA damage in lymphocytes occurred at uric acid concentrations of ≥600 μM. Adding catalase to the uric acid solution diminished the damaging effect, indicating that hydrogen peroxide mediated the prooxidant activity. Moreover, …