Biology Commons^™

Forest Recruitment Dynamics Within The Southern Boreal-Temperate Ecotone, Kiley M. Chernicky

2023 Midwest Ecology & Evolution Conference

Understanding how global climate change will alter future forest composition is especially challenging in forest communities where tree species have long co-occurred, but their competitive balance may be altered due to changes in climate. Of particular concern with increasing global temperatures are boreal forest communities that lie on the southern boreal-temperate forest ecotone, where the boreal forest is particularly vulnerable. The aim of my research is to investigate shifts in recruitment dynamics due to climate change near the southern range limit of two historically co-occurring boreal forest conifer species, white spruce (Picea glauca) and balsam fir (Abies …

Aquatic Vegetation Seed Bank Status Of Kentucky Lake, Emily A. Freeman

2023 Midwest Ecology & Evolution Conference

Aquatic vegetation is an important biotic component of reservoir ecosystems. Vegetation reduces erosion by acting as a barrier against current and waves from the lake. Aquatic vegetation also helps to oxygenate the water, stabilize nutrients like carbon, nitrogen, and phosphorus, and provides structure for aquatic vertebrates. The absence of vegetation in Kentucky Lake has been influenced by a fluctuating water table (due to winter lake drawdowns), invasive carp, and the use of herbicides for grass management. The objective of this study is to determine the state of the remaining seedbank within nautical mile markers 45 and 52. By using long-term …

Polyploidy’S Impact On Plant-Herbivore Dynamics: Evidence From Experiments With Water-Lily Aphids And Greater Duckweed, Hannah R. Assour

2023 Midwest Ecology & Evolution Conference

Polyploidy, or whole-genome duplication, is a widespread mutational process in plants with a myriad of phenotypic and genotypic consequences. In addition to consequences incurred by the plant, polyploidy can potentially cascade to alter species interactions. Yet, knowledge of whether ploidy-induced variation has effects on plant-herbivore dynamics is scarce. Here, we test whether aphid herbivores exhibit preference for a diploid or polyploid host cytotype and whether ploidy impacts plant resistance and tolerance to aphids, measured as aphid and plant performance respectively. To answer these questions, we evaluated aphid choice and compared the effects of the water-lily aphid (Rhopalosiphum nymphaeae) …

How Do Vernal Pond Temperature Fluctuations Impact Fitness Through Development?, Shayna Rosenbloom, Alycia Lackey

2023 Midwest Ecology & Evolution Conference

Anthropogenic climate change is driving shifts in natural temperature regimes. In addition to global warming, temperatures are becoming increasingly variable. Many organisms rely on seasonal temperatures across life stages as they grow and reproduce, among other critical aspects of their lives. A lot of work has been done on how increases in mean temperature might impact organisms, but there is a gap in our understanding on the impacts of increases in variation. Amphibians are especially susceptible to environmental change, particularly in earlier life stages. Increases in temperature have been shown to cause shifts in their phenology, decreases in body size, …

Evolution Of A Genus Of Gall Wasp Kleptoparasites, Guerin E. Brown

2023 Midwest Ecology & Evolution Conference

Kleptoparasites do not directly parasitize their hosts but instead steal food and resources, reducing host fitness. Like direct parasites, kleptoparasites can be highly dependent on their hosts such that their evolutionary histories may be linked. Here, we study the evolution of a kleptoparasitic wasp genus, Synergus (Hymenoptera: Cynipidae: Synergini), in relation to their hosts, oak gall wasps (Hymenoptera: Cynipidae: Cynipini). Ovipositing oak gall wasps induce the formation of galls on oaks (Fagaceae) that provide food and shelter to one or more developing gall wasps. Galls induced by different gall wasp species are diverse in size, shape, color, and location …

Where The Wild Mushrooms Are: Species And Secondary Metabolite Diversity In Fungal Species On The Saint Mary’S College Campus, Lex K. Gilman

2023 Midwest Ecology & Evolution Conference

Secondary metabolites in fungi have three main ecological functions: protection against predation, communication with other mushrooms, and assistance in pathogenic interactions. These compounds have also led to the common use of mushrooms as food and in traditional Chinese or other medicinal applications. However, there are currently few good resources for identifying mushroom species in North-Central Indiana. In addition, limited research exists on secondary metabolites in mushrooms. The objective of this research was to create a preliminary database of mushroom species at Saint Mary’s College and conduct preliminary research on the secondary metabolites of these local species. Fungal specimens were identified …

Dairy Bioactives And Functional Ingredients With Skin Health Benefits, Aleksandra Augustyniak, Davide Gottardi, Barbara Giordani, James Gaffey, Helena Mcmahon

Publications

The potential of whey from Italian cheese productions to support skin health and anti-ageing mechanism was studied. The effect of whey on dermal and epidermal cells was evaluated. Whey inhibited the activity of elastase and tyrosinase enzymes by 60% and 32%, respectively. Whey cytotoxicity against tested skin cell lines, human fibroblasts (HDFa) and keratinocytes (HaCaT) was not observed. Moreover, the antioxidant activity of the samples was noted, after treatment with whey intracellular ROS level was decreased by 87% in comparison with the hydrogen peroxide-treated cells. Fibroblasts produced a significant amount of extracellular matrix molecules, collagen I, elastin and glycosaminoglycans as …

Socio-Ecological Interactions Promote Outbreaks Of A Harmful Invasive Plant In An Urban Landscape, T. Trevor Caughlin, Matthew Clark, Louis W. Jochems, Nick Kolarik, Andrii Zaiats, Cody Hall, Jason M. Winiarski, Breanna F. Powers, Martha M. Brabec, Kelly Hopping

Biology Faculty Publications and Presentations

1. Urban landscapes often harbour organisms that harm people and threaten native biodiversity. These landscapes are characterized by differences in socioeconomic context, habitat suitability and patch connectedness. Identifying which spatial differences enable outbreaks of pests, pathogens and invasive species will improve targeted control efforts.

2. We tested hypotheses to explain the distribution and demography of puncturevine Tribulus terrestris, a human-dispersed invasive plant in Boise, a city in the western United States. We hypothesized an increase in puncturevine infestations near low-valued properties with a high proportion of bare ground, the species' preferred microhabitat, that are well connected on the urban road network. …

   ---

   Go to article

Memory Acquisition By Brainless Juvenile Terrestrial Planarians (Bipalium Kewense), Christina Chueiri, Christine Gildea

Transformations: Presentation Slides

Learning is how organisms build and store memories in their brains to aid them in survival. This process occurs as organisms experience new stimuli. Bipalium kewense, a terrestrial planarian, has a very minimal central nervous system. The nervous system of planarians consists of only two nerve cords and two ganglion which serve as a “brain.” This species reproduces by segmenting a small piece of their posterior end as a fragment, which initially lacks most adult structures. The fragments must regenerate the head, brain, mouth, functioning digestive system, and reproductive organs. To determine where memory is stored in these terrestrial planarians, …

Local Translation And Focal Adhesions Are Dysregulated In Down Syndrome, Ashlyn Gotberg, Nikita Kirkise M.S., Kristy Welshhans Ph.D.

Senior Theses

During cellular migration, fibroblasts sense extracellular cues through membrane complexes at their leading edge and can respond to these cues via local translation. Local translation is the process in which mRNAs are transported to subcellular regions and translated in response to specific cues. There is evidence that local translation may be dysregulated in the neurodevelopmental disorder, Down syndrome, which is caused by the triplication of chromosome 21 and results in genome-wide dysregulation of protein expression. To determine if dysregulated local translation contributes to Down syndrome, we used three sets of primary fibroblasts from individuals with Down syndrome and apparently healthy …

Using Podcasts To Bring National Estuarine Research Reserves Into The Classroom For Grades 6-12, Kaitlyn M. Dirr

Senior Theses

In a typical classroom setting, there are significant challenges to exposing students to concepts related to earth sciences and the environment. These challenges are exacerbated when conveying lessons about geographic areas with limited access, such as oceans and coastlines (Louv, 2010). It is now more important than ever for environmental education to improve and adapt to our changing world. Educators may have the opportunity to bring these subjects to life by using media content such as podcasts to introduce students to new places and the scientists, managers and educators that work in those spaces. In the United States, there exists …

Coordination Of Hydraulic And Morphological Traits Across Dominant Grasses In Eastern Australia, Robert J. Griffin-Nolan, Jeff Chieppa, Alan K. Knapp

Biology

1. Leaf hydraulic traits characterize plant drought tolerance and responses to climate change. Yet, plant hydraulics are biased towards northern hemisphere woody species. We collected rhizomes of several perennial grass species along a precipitation gradient in eastern Australia and grew them in an experimental pot study to investigate potential trade-offs between drought tolerance and plant morphology.
2. We measured the following leaf hydraulic traits: the leaf water potential (Ψleaf) at 50% and 88% loss of leaf hydraulic conductance (P50Kleaf and P88Kleaf), the Ψleaf at 50% loss of stomatal conductance (P50gs), leaf turgor loss point (TLP), leaf dry matter content (LDMC), leaf modulus …

Effects Of Heavy Metal Uptake On Growth, Chlorophyll Content, And Calcium Oxalate Crystals In Lemna Minor (Duckweed), Olivia F. Miller

Undergraduate Honors Thesis Projects

Lemna minor L., duckweed, is a common aquatic plant used for the phytoremediation of wastewater. Heavy metal contamination poses a significant issue, and numerous studies have been conducted on the efficiency of L. minor’s hyperaccumulation ability of these metals. Calcium oxalate crystals are an important aspect of the growth and development of L. minor, but how they are influenced by the uptake of heavy metal has not been extensively studied. This study aims to determine the effects that lead, cadmium, and copper have on the formation of calcium oxalate crystals, vegetative growth, and chlorophyll a and b content on L. …

Measuring Claim-Evidence-Reasoning Using Scenario-Based Assessments Grounded In Real-World Issues, William L. Romine, Amy Lannin, Ankita Agarwal, Maha Kareem, Emily Burwell

Biological Sciences Faculty Publications

Improving students’ use of argumentation is front and center in the increasing emphasis on scientific practice in K-12 Science and STEM programs. We explore the construct validity of scenario-based assessments of claim-evidence-reasoning (CER) and the structure of the CER construct with respect to a learning progression framework. We also seek to understand how middle school students progress. Establishing the purpose of an argument is a competency that a majority of middle school students meet, whereas quantitative reasoning is the most difficult, and the Rasch model indicates that the competencies form a unidimensional hierarchy of skills. We also find no evidence …

Could Biological Soil Crusts Act As Natural Fire Fuel Breaks In The Sagebrush Steppe?, Lea A. Condon, Douglas J. Shinneman, Roger Rosentreter, Peter S. Coates

Biology Faculty Publications and Presentations

For decades, large portions of the semi-arid sagebrush ecosystem have been experiencing increased frequency and extent of wildfire, even though small, infrequent fire is a natural disturbance in this ecosystem (Baker, 2006). Increased wildfire is threatening the existence of sagebrush ecosystems and the wildlife species that depend upon them (Baker, 2006; Coates et al., 2016). Increased wildfire in sagebrush ecosystems is often driven by invasive annual grasses, especially cheatgrass, Bromus tectorum (L.). Invasion can initiate a trajectory toward a “grass-fire cycle”, in which cheatgrass increases fine fuel loadings that promote fire, and native plant species do not recover quickly after …

Positive And Relaxed Selective Pressures Have Both Strongly Influenced The Evolution Of Cryonotothenioid Fishes During Their Radiation In The Freezing Southern Ocean, Kevin T. Bilyk, Xuan Zhuang, Chiara Papetti

Department of Biology Faculty Scholarship and Creative Works

Evolution in the chronic cold of the Southern Ocean has had a profound influence on the physiology of cryonotothenioid fishes. However, the suite of genetic changes underlying the physiological gains and losses in these fishes is still poorly surveyed. By identifying the genomic signatures of selection, this study aims to identify the functional classes of genes that have been changed following two major physiological transitions: the onset of freezing temperatures and the loss of hemoproteins. Looking at the changes that followed the onset of freezing temperatures, positive selective pressure was found among a set of broadly acting gene regulatory factors, …

In Vitro Characterization Of Fluorogenic Chemical Tools To Study Human Carboxylesterases (Cess), Taylor Parish Spidle

Undergraduate Honors Theses

Human carboxylesterases (CESs) are enzymes that are responsible for the metabolism of many important pharmaceuticals. Although CESs are key players in the hydrolysis of many ester-containing drugs, they remain understudied. Our group hypothesizes that this is primarily because there are few methods capable of reporting activity in live cells. Here, I report a new series of fluorogenic chemical tools to study the CES activity of one of the two major CESs in humans, CES1, in live cells. MCP-Me, MCP-Et, and MCP-iPr utilize the same carbonate group of a previously developed chemical tool in our group, FCP-1, and work to mimic …

Diet Of A Threatened Rattlesnake (Eastern Massasauga) Revealed By Dna Metabarcoding, Alyssa Swinehart, Charlyn Partridge, Amy L. Russell, Arin Thacker, Jennifer Kovach, Jennifer A. Moore

Open Access Publishing Support Funded Articles

Characterizing the diet of imperiled species using minimally invasive methods is crucial to understanding their ecology and conservation requirements. Here, we apply a DNA metabarcoding approach to study the diet of the eastern massasauga rattlesnake (Sistrurus catenatus), a Federally Threatened snake found throughout the Great Lakes region. Eighty-three fecal samples collected across 10 different massasauga populations located in Michigan, USA, were sequenced, with 70 samples containing prey DNA. We used universal metazoan primers and developed a host-specific oligonucleotide blocker to characterize their diet. We identified at least 12 different prey species, with eastern massasaugas exhibiting opportunistic feeding and …

Life Line Spring 2023, Otterbein Biology And Earth Science Department

Life Line - The Biology Department Newsletter

In this Issue:

Feathered Friends Living on Campus; The Hidden Mussels of the Olentangy River; Early Assurance Program Students Graduate from HCOM; Students Present at American Society for Microbiology Microbe 2022 Meeting; Environmental Science Upgrades Its Remote Monitoring Capabilities; Speed!; UK Students Visit Local Attractions; That's a Lottl Axolotls.

Mir125a As Molecular Target Of Vitamin D Mediated Inhibition Of Cd4+ T Cell Differentiation Into Th17 Cells In Mrl/Lpr Lupus-Prone Mice, Miranda Hilt

Undergraduate Distinction Papers

The dynamic function of vitamin D in an array of immunomodulatory and anti-inflammatory mechanisms and its implication in the pathogenesis of autoimmune diseases has been the topic of much recent scholarship. Here I attempt to elucidate the molecular mechanism of vitamin D-mediated inhibition of the differentiation of CD4+ T cells into pro-inflammatory Th17 cells in MRL/lpr lupus prone mice by investigating how miR-125a expression is affected by dietary modulation of cholecalciferol (vitamin D₃). MRL/MpJ and MRL/lpr mice were split into three experimental groups and fed specially formulated diets that varied in their concentrations of vitamin D. …

Challenges In Forecasting Antimicrobial Resistance, Sen Pei, Seth Blumberg, Jaime Cascante Vega, Tal Robin, Yue Zhang, Richard J. Medford, Bijaya Adhikari, Jeffrey Shaman

Research on Antimicrobial Resistance

Antimicrobial resistance is a major threat to human health. Since the 2000s, computational tools for predicting infectious diseases have been greatly advanced; however, efforts to develop real-time forecasting models for antimicrobial-resistant organisms (AMROs) have been absent. In this perspective, we discuss the utility of AMRO forecasting at different scales, highlight the challenges in this field, and suggest future research priorities. We also discuss challenges in scientific understanding, access to high-quality data, model calibration, and implementation and evaluation of forecasting models. We further highlight the need to initiate research on AMRO forecasting using currently available data and resources to galvanize the …

The Possible Role For Basigin Receptor Function In The Induction Of Matrix Metalloproteinase Expression In Human Endometrial Stromal Cells, Amy Abel

All NMU Master's Theses

10-15% of couples worldwide are affected by infertility. The leading cause of infertility is implantation failure. Molecular communication in precise time and space between an implanting embryo and a receptive uterus is essential for successful implantation. Basigin has been shown to have vital functions within the implantation process. Basigin is best known for inducing the expression of matrix metalloproteinases. Basigin has been shown to induce MMP expression in vitro in human endometrial stromal cell lines (HESC). Understanding how basigin induces matrix metalloproteinases within normal cellular conditions will help researchers understand how these signaling cascades flow in the framework of implantation. …

The Status Of Invasive Dusky Slug (Arion) Species In The Upper Great Lakes: A Molecular Approach, Olivia W. Hall

All NMU Master's Theses

I update the distribution of two cryptic slug species, Arion fuscus and Arion subfuscus, in the Upper Great Lakes of the United States using molecular identification methods. Arion fuscus has not previously been reported in the literature in this region, and Arion subfuscus has previously been reported as abundant. However, all previous distribution studies were determined using visual identification tools, which can result in misidentification between cryptic species. To molecularly re-examine these distribution maps, I used a mitochondrial ribosomal subunit 16S PCR amplification and subsequent SSpI and MfeI restriction enzyme digest to identify slug specimens. I collected slugs in …

Targeted Mapping Of Oryza Sativa Chilling Tolerance Candidate Genes Using Multiple Stress-Relevant Quantitative Traits, Naoki Simon Shimoyama

Dissertations (1934 -)

Developing chilling tolerant accessions of domesticated Asian rice is a potential source of significant crop improvement to address the needs of a growing global population. The uniquely chilling sensitive nature of the tropically originating Oryza sativa make it the most important staple crop that could gain the maximum benefit from improved tolerance to low temperature stress. However, mechanisms underlying this complex trait are not fully understood. Oryza sativa has two major varietal groups with different levels of chilling tolerance, JAPONICA and INDICA, providing an ideal tool to investigate mechanistic differences in the chilling stress tolerance responses within this important crop …

A Review Of The Forensic Applications For The Analysis Of Biological Fluids Using Fluorescence Spectroscopy, Raghavi Patel

Biological Sciences

The use of science to compile information to assist in solving crimes is known as forensic science. Numerous bodily fluids, such as blood, semen, saliva, and vaginal fluid, are frequently discovered at crime scenes. This makes it crucial for forensic scientists to use analytical methods to examine these fluids to learn more about the crime, including the offender, victim, time since deposition, and other details. Although there are many techniques, the focus of this paper is fluorescence spectroscopy. Fluorescence spectroscopy is a rapid, inexpensive, and non-destructive technique used for forensic examination (Weber & Lednev, 2022). Fluorescence spectroscopy can be used …

Evidence That The Inner Nuclear Membrane Is A Functional Β1-Adrenergic Receptor Signaling Platform In Astrocytes, Kelsey Benton

Dissertations (1934 -)

The noradrenergic system exerts powerful modulatory influences over fundamental brain processes including attention, arousal, learning, and memory by activating adrenergic receptors on neuronal and glial cells. In addition to triggering rapid, transient changes in cellular physiology that drive short-term behavioral actions, these G-protein-coupled receptors also initiate delayed, long-lasting changes in cellular function by regulating gene expression. For example, in astrocytes, norepinephrine-induced activation of β-adrenergic receptors (β-ARs) rapidly induces glucose mobilization from glycogen stores, while simultaneously initiating delayed changes in the expression of glycogen storage genes. Regulation of gene expression requires adrenergic receptor-initiated intracellular signals to reach the nuclear compartment. To …

Altered Motivation & Dopamine Subcircuit Plasticity Unique To Sex During Protracted Opioid Withdrawal, Devan Marc Gomez

Dissertations (1934 -)

Opioid use disorder (OUD) is associated with enduring psychological withdrawal symptoms believed to contribute to drug abuse. Amongst these are shifts in motivational states, wherein pursuit of drug consumption exceeds that of non-drug rewards, reinforcing escalated opioid use and relapse vulnerability. A critical regulator of behavioral reinforcement, the mesoaccumbal dopamine system is thought to be both necessary and sufficient for opioid motivation. However, previous research into its involvement in opioid withdrawal has been limited to acute rather than protracted timepoints, global neuroadaptations rather than those in subcircuits, and overwhelmingly focused on males over females. Using a rodent model of self-administration …

Postnatal Developmental Trends In Membrane Excitability And Bk Channel Function In The Rodent Hippocampus, Michael S. Hunsberger

Dissertations (1934 -)

This dissertation outlines the postnatal development of excitability as well as expression and function of BK potassium channels in hippocampal neurons. I used patch clamp electrophysiology to measure how neuronal action potential waveforms and action potential firing frequencies change in early development, and how pharmacological blockade of BK channels affects these properties in hippocampal neurons. I also describe how the protein expression of the BK channel pore-forming α subunit and mRNA expression of different variants of the pore forming α subunit and auxiliary beta-4 subunit changes with development. I demonstrate in both cultured rat hippocampal neurons across the first seven …

Genetic Mechanisms Underlying Maladaptation In Specialized Species Interactions, Nitin Ravikanthachari

Theses and Dissertations

Adaptation has been used as the framework to understand the power of natural selection in structuring biodiversity and driving biological interactions. However, recent examples have indicated that many organisms are maladapted to their local conditions. Maladaptation can result from various causes including rapid environmental change, novel environments, interplay of evolutionary forces (viz: gene flow-selection balance) and anthropogenic disturbance.

To dissect the genetic mechanisms underlying maladaptation, I used the interaction between Pieris macdunnoughii (Remington) (Lepidoptera: Pieridae) which lays eggs on an invasive mustard Thlaspi arvense (L.) (Brassicaceae), which is lethal to the larvae.

First, local adaptation to avoid laying eggs on …

The Effect Of Bisphenol A (Bpa) Substitutes On Cd4+ T Cell Immunity, Cassandra Poole

Longwood Senior Theses

CD4+ helper T cells are an important cell of the immune system. One key factor in the mediation of the immune system in general, and T cells in particular, is the female sex hormone estrogen. Abnormal estrogen levels are associated with a variety of conditions, including autoimmune diseases, and several types of cancer, including breast cancer. BPA is a well-known estrogen mimicking compound, used in the manufacturing of plastics, epoxy resins, and polystyrenes. As the negative effects of BPA become more apparent, many BPA substitutes have been developed. While the effect of estrogen on T cells is understood, the effect …