Biology Commons^™

The Relationship Between Maternal Plasma Homocysteine In Early Pregnancy And Birth Weight, Shona Cawley, Eimer O'Malley, Rachel Kennedy, Ciara Reynolds, Anne Molloy, Michael Turner

Articles

Background: There is limited evidence that plasma homocysteine (Hcy) is increased in women with adverse pregnancy outcomes, such as low birth weight (LBW).

Objective: We examined the relationship between maternal Hcy at the first prenatal visit and birth weight.

Study design: In a prospective observational study, women were recruited during their first prenatal visit after sonographic confirmation of gestational age. Along with the standard tests, blood was also taken for the measurement of maternal serum and red blood cell (RBC) folate, vitamin B12, and Hcy. In addition to collecting standard clinical and sociodemographic details, a detailed questionnaire on vitamin supplementation …

Subjective And Objective Assessment Of Taste And Smell Sensation In Advanced Cancer, Niamh Mcgettigan, Pauline Uí Dhuibhir, Michelle Barrett, Jessica Sui, Lucy Balding, Stephen Higgins, Norma O'Leary, Aileen Kennedy, Declan Walsh

Articles

Context: Taste and smell abnormalities (TSA) occur throughout the cancer trajectory regardless of cancer primary site and contribute to cancer-associated malnutrition. TSA etiology is poorly understood. Tumor-related inflammation is a possible cause. Objective: This study examined the prevalence, characteristics, and severity of TSA in advanced cancer and explored the relationship between TSA and nutritional status. No previous study combined subjective and objective measures for both taste and smell assessment in this population. Method: Consecutive advanced cancer hospice patients were recruited. A modified version of the “Taste and Smell Survey” assessed subjective TSA. Validated taste strips and “Sniffin’ …

Ancient Origins Of A Diverse Stygofaunal Group: The Australian Bathynellidae (Crustacea), Giulia Perina

Theses: Doctorates and Masters

Research on subterranean fauna on all continents has highlighted often high levels of endemism, for both troglobionts (terrestrial subterranean organisms) and stygobionts (organisms living in groundwater). In Australia, particularly in the arid zones of Western Australia, a rich hypogean assemblage has been discovered in the past few decades, largely due to surveys required as part of environmental impact assessments for, and imposed conditions on, mining operations. Bathynellidae (Crustacea) are part of the stygofaunal community, but to-date they are poorly understood, mainly because their conservative morphology, small size and delicate exoskeleton make their dissection, observation and study very difficult. Additionally, the …

Differential Activity Of Coexisting Prochlorococcus Ecotypes, Anne Thompson, Kathleen Kouba

Biology Faculty Publications and Presentations

The abundance and widespread distribution of the unicellular cyanobacterium Prochlorococcus is attributed to its extensive genetic diversity. At least twelve distinct clades, or ecotypes, of Prochlorococcus have been discovered so far, and follow distinct distribution patterns over horizontal and vertical gradients in the oligotrophic ocean. However, multiple ecotypes (and sub-ecotypes) of Prochlorococcus always coexist in varying proportions. While laboratory experiments and genomic analysis suggest that ecotypes contribute differently to biogeochemical cycles, no studies have tested the differential activity of coexisting ecotypes in natural assemblages of Prochlorococcus. Here, we performed DNA stable isotope probing (DNA-SIP) experiments in on-deck incubations of oceanic …

Mitosrnas And Extreme Anoxia Tolerance In Embryos Of The Annual Killifish Austrofundulus Limnaeus, Claire L. Riggs, Steven Cody Woll, Jason Podrabsky

Biology Faculty Publications and Presentations

Embryos of the annual killifish Austrofundulus limnaeus are the most anoxia-tolerant vertebrate. Annual killifish inhabit ephemeral ponds, producing drought and anoxia-tolerant embryos, which allows the species to persist generation after generation. Anoxia tolerance and physiology vary by developmental stage, creating a unique opportunity for comparative study within the species. A recent study of small ncRNA expression in A. limnaeus embryos in response to anoxia and aerobic recovery revealed small ncRNAs with expression patterns that suggest a role in supporting anoxia tolerance. MitosRNAs, small ncRNAs derived from the mitochondrial genome, emerged as an interesting group of these sequences. MitosRNAs derived from …

Spatiotemporal Dynamics Of Nitrogen And Carbon Biogeochemistry In A Wetland-Stream Sequence, Patrick E. Hurley

Graduate Student Theses, Dissertations, & Professional Papers

Studies of aquatic ecosystems often segregate streams from the influential ponds, lakes, and wetland zones that act as important transitions between terrestrial and fluvial systems. Across the aquatic landscape, these zones interact to form linked ecosystems that function as discrete nutrient processing domains, shifting biogeochemical signals due to spatial and temporal variability in hydrologic and biologic controls. Using a mass-balance approach, we profiled nutrient dynamics along a 23-km wetland-stream sequence over three seasons. Hydrologic, morphologic, and biologic conditions, as well as landscape attributes, were quantified to determine potential controls on biogeochemical cycling in a tributary of the Upper Clark Fork …

Population And Breeding Ecology Of Sagebrush Steppe Songbirds, Kayla Ann Ruth

Graduate Student Theses, Dissertations, & Professional Papers

Sagebrush steppe is one of the most threatened ecosystems in North America. Domestic livestock grazing is the dominant land use of sagebrush steppe across the west. Rest-rotation grazing systems can be a conservation management tool, most recently, by the Natural Resource Conservation Service - Sage Grouse Initiative (SGI). The goal of SGI is to encourage private landowners to use a livestock grazing regime that maintains or improves habitat for greater sage-grouse (Centrocercus urophasianus), as well as improve rangeland productivity. Songbirds are biological indicators that can assess the health of sagebrush steppe. Avian adult density estimates are often used …

Methods For Estimating Mountain Goat Occupancy And Abundance, Molly Mcdevitt

Graduate Student Theses, Dissertations, & Professional Papers

Abundance and occupancy are two parameters of central interest to the field of ecology. Furthermore, accurate (both precise and unbiased) estimates are key pieces to the puzzle of effective wildlife management decision-making. While there exist a variety of sampling techniques and statistical models for effectively estimating population parameters for frequently encountered and large mammals, methods for sampling unmarked and rare species are few and far between. The first step to acquiring usable parameter estimates is through the use of sampling theory and incorporation of probabilistic sampling designs to collect count-data and occurrence-data. Often, it is assumed that probabilistic sampling designs …

Density And Distribution Of Piscivorous Fishes In The Sacramento – San Joaquin Delta, Christopher M. Loomis

Cal Poly Humboldt theses and projects

Predatory fishes, including numerous introduced species, are common to the Sacramento – San Joaquin Delta, but abundance data for most species is insufficient to determine the Delta-wide distribution and ecological impacts of these species. Predatory fishes (e.g. Striped Bass, Largemouth Bass have long been suspected of contributing to the decline of native species, including salmonids, but data has been insufficient to investigate this hypothesis. In this study, I present a novel method to assess predator fish populations across the southern Delta using DIDSON acoustic cameras and analyze the environmental associations that form the landscape and fine-scale distribution of predatory fishes. …

Life History Of The Coastal Tailed Frog (Ascaphus Truei) Across An Elevational Gradient, Adrian Daniel Macedo

Cal Poly Humboldt theses and projects

The life history of a species is described in terms of its growth, longevity, and reproduction. Unsurprisingly, life history traits are known to vary in many taxa across environmental gradients. In the case of amphibians, species at high elevations and latitudes tend to have shorter breeding seasons, shorter activity periods, longer larval periods, reach sexual maturity at older ages, and produce fewer and larger clutches per year.

The Coastal Tailed Frog (Ascaphus truei) is an ideal species for the study of geographic variation in life history because it ranges across most of the Pacific Northwest from northern California …

Spatial Variability In Recruitment Of Chilipepper Rockfish (Sebastes Goodei) In The California Current System, Laura K. Solinger

Cal Poly Humboldt theses and projects

Properly describing variability in population dynamics (e.g., growth, fecundity, recruitment) is expected to improve management of important fisheries stocks (Maunder & Piner, 2014). As recruitment to most fish stocks is determined during early life history stages (Houde, 1997; Iles & Beverton, 2000), and early life history stages are influenced by oceanographic conditions (Bjorkstedt et al. 2002; Laidig, 2010), understanding how environmental stochasticity influences recruitment deviations has potential to improve estimates of stock productivity and how productivity might change over time to support more effective management. Chilipepper Rockfish (Sebastes goodei) are an important commercial species that is managed as …

Freshwater And Marine Survival Of Coho Salmon (Oncorhynchus Kisutch) As A Function Of Juvenile Life History, Grace Katherine Ghrist

Cal Poly Humboldt theses and projects

Juvenile Coho Salmon (Oncorhynchus kisutch) in coastal California streams exhibit various life history strategies during their freshwater development. One strategy of interest to managers and conservationists is the early migrant. Juvenile early migrants emigrate from natal habitat into lower parts of the watershed or estuary during their first fall or winter, where they rear before migration to the ocean. By contrast, the more prevalent spring migrant resides in natal reaches over the winter and migrates directly to the ocean the following spring. Salmon monitoring programs generally estimate juvenile production and demographic rates using only spring migrants, and these …

Response Of Breast Cancer Cells To Ifnî±-2b In 2d And 3d Cell Cultures, Tetiana Herheliuk, Olena Perepelytsina, Andrij Ugnivenko, Lyudmila Ostapchenko, Mychailo Sydorenko

Turkish Journal of Biology

The effect of IFNα-2b on the migration, proliferation, and expression of epithelial and mesenchymal markers of MCF-7 tumor adenocarcinoma cells in 2D and 3D cell cultures was examined. A significant cytostatic effect of IFNα-2b on the tumor population was detected. It was found that changes in the expression of epithelial (CKs and EpCAM) and mesenchymal markers were caused by changing the growth type of the tumor population. IFNα-2b inhibited migration of tumor cells to the suspension fraction and promoted an increase in expression of CK and EpCAM in 2D and 3D cell cultures, but only in the 3D culture was …

Transcriptome Analysis Reveals Differentially Expressed Genes Between Human Primary Bone Marrow Mesenchymal Stem Cells And Human Primary Dermal Fibroblasts, Eki̇m Z. Taşkiran, Beren Karaosmanoğlu

Turkish Journal of Biology

Stromal cells have been widely used in biomedical research and disease modeling studies in vitro. The most commonly used types of stromal cells are mesenchymal stem cells and fibroblasts. Their cellular phenotypes and differentiation capabilities are quite similar and there are no specific distinction criteria. In order to identify transcriptomic differences between these 2 cell types, we performed next-generation RNA sequencing. Using the global gene expression profile and pathway analysis, we showed that human primary bone marrow mesenchymal stem cells and human primary dermal fibroblasts have different molecular signatures. We also identified critical transcription factors that are differentially expressed between …

Genome And Metabolome Mining Of Marine Obligate Salinispora Strains To Discover New Natural Products, Süleyman Özakin, Ebru İnce Bostanci

Turkish Journal of Biology

Marine microorganisms are receiving more attention as a promising potential source of new natural products. In the present study, we performed genomic and metabolomic analyses to explore the metabolic potential of the obligate marine actinomycete genus Salinispora. The genomes of thirty Salinispora strains were prospected in search of biosynthetic gene clusters including polyketide synthase (PKS), nonribosomal peptide synthetase (NPRS), terpene, indole, lantibiotics, and siderophores. We determined considerable diversity of natural product biosynthetic gene clusters in their genome. There were a total of 1428 putative gene clusters involved in the biosynthesis of various bioactive natural products. Furthermore, 1509 ketosynthase (KS) and …

Comparison Of Indigo Carmine Decolorization By Pseudomonas Aeruginosa And Crude Laccase Enzyme From Funalia Trogii, Fi̇li̇z Boran, Emre Bi̇rhanli, Özfer Yeşi̇lada, Eli̇f Özbey

Turkish Journal of Biology

The effects of incubation time, temperature, initial pH, and dye concentration on the indigo carmine decolorization activity of Pseudomonas aeruginosa ATCC 10145 and some factors on the decolorization potential of crude laccase enzyme obtained from Funalia trogii ATCC 200800 were comparatively investigated. This bacterium showed effective decolorization activity at all agitation and temperature values. Indigo carmine was greatly decolorized by P. aeruginosa at all pH values except pH 10. A decrease in decolorization activity occurred with increasing dye concentration, but this bacterium effectively decolorized the dye within 24 h. The decolorization process was through microbial metabolism, not biosorption. No decolorization …

Morphophysiological And Biochemical Response Of Mungbean [Vigna Radiata (L.) Wilczek] Varieties At Different Developmental Stages Under Drought Stress, Pooja Bangar, Ashok Chaudhury, Bhavana Tiwari, Sanjay Kumar, Ratna Kumari, Kangila Venkataramana Bhat

Turkish Journal of Biology

The present study was conducted to assess the morphophysiological and biochemical responses during different developmental stages in mungbean varieties subjected to drought stress, and to screen the varieties for drought tolerance. A field experiment was performed according to a completely randomized design on 25 mungbean varieties with 3 replicates per variety. Stress treatment was applied at 3 levels: control (no stress), vegetative stage (25 days after sowing), and reproductive stage (35 days after sowing). According to combined analysis of variance, there were significant effects from drought stress on relative water content (RWC), membrane stability index (MSI), protein and proline content …

Development Of Antimicrobial Gelatin Films With Boron Derivatives, Sanem Argin, Merve Güleri̇m, Fi̇kretti̇n Şahi̇n

Turkish Journal of Biology

Food packaging technology has been advancing to provide safe and high quality food products and to minimize food waste. Moreover, there is a dire need to replace plastic materials in order to reduce environmental pollution. The aim of this study was to prepare biodegradable antimicrobial packaging films from gelatin. Boric acid, disodium octaborate tetrahydrate, and sodium pentaborate were incorporated as the antimicrobial agents. Films containing boric acid and its salts showed antibacterial effect against Staphylococcus aureus and Pseudomonas aeruginosa, as well as antifungal and anticandidal effects against Aspergillus niger and Candida albicans. The mechanical strength of the films was mostly …

Effects Of Notch Signalling On The Expression Of Sema3c, Hmga2, Cxcl14, Cxcr7, And Ccl20 In Breast Cancer, Cansu Küçükköse, Özden Yalçin Özuysal

Turkish Journal of Biology

Metastasis is the main reason for death in breast cancer. Understanding the molecular players in metastasis is crucial for diagnostic and therapeutic purposes. Notch signalling plays an oncogenic role in breast tumorigenesis and is involved in metastasis. Downstream mediators of Notch signalling in prometastatic processes are not yet fully discovered. Here we aimed to investigate whether Notch signalling regulates the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20, which are involved in prometastatic processes, in breast cell lines. To this end, expression of the selected genes was analysed following Notch activation by overexpression of the Notch1 intracellular domain in the …

Complete Genome Sequence Analysis Of A Lytic Shigella Flexneri Vb_Sfls-Isf001 Bacteriophage, Khashayar Shahin, Majid Bouzari, Ran Wang

Turkish Journal of Biology

Shigellosis is one of the most important acute enteric infections caused by different species of Shigella, such as Shigella flexneri. Despite the use of antibiotic therapy to reduce disease duration, this approach is becoming less effective due to the emergence of antibiotic resistance among Shigella spp. Bacteriophages have been introduced as an alternative for controlling shigellosis. However, the bacteriophages must be without any lysogenic or virulence factors, toxin coding, or antibiotic-resistant genes. In this study, the whole genome sequence of vB_SflS-ISF001, a virulent Siphoviridae bacteriophage specific for Shigella flexneri, was obtained, and a comparative genomic analysis was carried out to …

Contribution Of Hepatitis B Virus X Protein-Induced Aberrant Microrna Expression To Hepatocellular Carcinoma Pathogenesis, Zhiyuan Wei, Xiaohe Shen, Bing Ni, Gaoxing Luo, Yi Tian, Yi Sun

Turkish Journal of Biology

The hepatitis B virus-encoded X (HBX) protein plays important roles in Hepatocellular carcinoma (HCC). Previous studies have demonstrated that HBX can induce alterations in the expression of numerous microRNAs (miRNAs) involved in the carcinogenesis of various tumors. However, the global profile of liver miRNA changes induced by HBX has not been characterized. In this study, we conducted a miRNA microarray analysis to investigate the influence of HBX on the expression of total miRNAs in liver in relation to HCC. Comparative analysis of the data from human normal liver cells (L02) and human HCC cells (HepG2), with or without HBX, identified …

Identification Of Bioactive Compounds From Fraxinus Angustifolia Extracts With Anti-Nadh Oxidase Activity Of Bovine Milk Xanthine Oxidoreductase, Nadjia Ahmane, Dina Atmani-Kilani, Nassima Chaher, Karima Ayouni, Meriem Rahmani-Berboucha, Gregory Da Costa, Nadjet Debbache-Benaida, Tristan Richard, Djebbar Atmani

Turkish Journal of Biology

Fraxinus angustifolia leaves and bark are used in traditional medicine against various inflammatory-related pathologies incumbent to reactive oxygen species (ROS) generation by the NADH oxidase activity of enzymes such as xanthine oxidoreductase (XOR). This study was designed to investigate the in vitro and in vivo inhibitory activities of this enzyme by Fraxinus angustifolia extracts. The leaf organic phase of ethyl acetate (LFA) and its bark aqueous counterpart (BFA) showed the strongest anti-NADH oxidase activity in vitro (IC50 = 38.51 and 42.04 μg mL-1, respectively). They consequently suppressed superoxide generation both enzymatically (53% and 19%, respectively) and nonenzymatically (34% and 19%, …

Functional Characteristics Of Lactobacilli From Traditional Bulgarian Fermented Milk Products, Veronica Nemska, Petya Logar, Tanya Rasheva, Zdravka Sholeva, Nelly Georgieva, Svetla Danova

Turkish Journal of Biology

After oral administration, probiotic lactobacilli meet a number of protection systems in the human body, such as exposure to gastric, pancreatic, and small intestinal juices. Overcoming these detrimental barriers allows living bacteria to adhere to the intestinal epithelium and permanently colonize the gastrointestinal tract (GIT), providing health benefits to the host. Based on this, the transit tolerance of 25 candidate probiotic lactobacilli from katak, yoghurt, and white-brined and yellow cheese to simulated bile and small intestinal juices of variable pH was investigated. To establish their resistance, in vitro model systems based on modified MRS media and a longer duration of …

Key Actors In Cancer Therapy: Epigenetic Modifiers, Remzi̇ Okan Akar, Seli̇n Selvi̇, Engi̇n Ulukaya, Nazlihan Aztopal

Turkish Journal of Biology

Epigenetic reprogramming plays a crucial role in the tumorigenicity and maintenance of tumor-specific gene expression that especially occurs through DNA methylation and/or histone modifications. It has well-defined mechanisms. It is known that alterations in the DNA methylation pattern and/or the loss of specific histone acetylation/methylation markers are related to several hallmarks of cancer, such as drug resistance, stemness, epithelial-mesenchymal transition, and metastasis. It has also recently been highlighted that epigenetic alterations are critical for the regulation of the stemlike properties of cancer cells (tumor-initiating cells; cancer stem cells). Cancer stem cells are thought to be responsible for the recurrence of …

Characterization Of Mesenchymal Stem Cells In Mucolipidosis Type Ii (I-Cell Disease), Sevi̇l Köse, Fati̇ma Aerts Kaya, Bülent Bariş Kuşkonmaz, Fahri̇ye Duygu Çeti̇nkaya

Turkish Journal of Biology

Mucolipidosis type II (ML-II, I-cell disease) is a fatal inherited lysosomal storage disease caused by a deficiency of the enzyme N-acetylglucosamine-1-phosphotransferase. A characteristic skeletal phenotype is one of the many clinical manifestations of ML-II. Since the mechanisms underlying these skeletal defects in ML-II are not completely understood, we hypothesized that a defect in osteogenic differentiation of ML-II bone marrow mesenchymal stem cells (BM-MSCs) might be responsible for this skeletal phenotype. Here, we assessed and characterized the cellular phenotype of BM-MSCs from a ML-II patient before (BBMT) and after BM transplantation (ABMT), and we compared the results with BM-MSCs from a …

Squalene Attenuates The Oxidative Stress And Activates Akt/Mtor Pathway Against Cisplatin-Induced Kidney Damage In Mice, Ayşe Arzu Şakul, Mehmet Ozansoy, Bi̇rsen Eli̇bol, Şule Ayla, Mehmet Yalçin Günal, Yasemi̇n Yozgat, Selma Hüveyda Başağa, Kazi̇m Şahi̇n, Rümeyza Kazancioğlu, Ülkan Kiliç

Turkish Journal of Biology

The clinical use of cisplatin, which is a first-line anticancer agent, is highly restricted due to its adverse effects on kidneys that lead to nephrotoxicity. Therefore, some potential reno-protective substances have been used in combination with cisplatin to cope with nephrotoxicity. Due to its high antitumor activity and oxygen-carrying capacity, we investigated the molecular effects of squalene against cisplatin-induced oxidative stress and kidney damage in mice. Single dose of cisplatin (7 mg/kg) was given to male Balb/c mice. Squalene (100 mg/kg/day) was administered orogastrically to mice for 10 days. Following sacrification, molecular alterations were investigated as analysis of the levels …

Examining The Involvement Of Slx5 In The Apoptotic Response To Chronic Activation Of The Spindle Assembly Checkpoint, Pinar Buket Atalay, Eli̇f Ergi̇n Çavuşoğlu, Öykü Aşci, Duygu Aygüneş

Turkish Journal of Biology

Microtubule-targeting agents represent one of the most successful groups of anticancer drugs used in cancer therapy today. These drugs induce a prolonged mitotic arrest through chronic spindle assembly checkpoint (SAC) activation. Apoptosis, an outcome of the prolonged mitotic arrest, is the main mechanism by which these anticancer drugs kill cancer cells. However, not much is known about the mechanism that directs chronic SAC activation to apoptosis among other possible outcomes. The aim of this study is to investigate whether Slx5, a sumo-targeted ubiquitin E3 ligase, is involved in directing chronic SAC activation to apoptosis. We show that chronic SAC activation …

Precipitation And Characterization Of Caco3 Of Bacillus Amyloliquefaciens U17 Strain Producing Urease And Carbonic Anhydrase, Merve Tepe, Şevki̇ Arslan, Tamer Koralay, Nazi̇me Mercan

Turkish Journal of Biology

In the present study, the properties of calcium carbonate mineralization and urease and carbonic anhydrase activities of Bacillus amyloliquefaciens U17 isolated from calcareous soil of Denizli (Turkey) were analyzed. CaCO3 was produced in all growth phases. Strain U17 showed 0.615 ± 0.092 μmol/min/mg urease enzyme activity in calcium mineralization medium and 1.315 ± 0.021 μmol/min/mg urease enzyme activity in Luria-Bertani medium supplemented with urea, whereas it showed 36.03 ± 5.48 nmol/min/ mg carbonic anhydrase enzyme activity in CaCO3 precipitation medium and 28.82 ± 3.31 nmol/min/mg carbonic anhydrase enzyme activity in Luria-Bertani medium supplemented with urea. The urease B protein expression …

Effects Of B2o3 (Boron Trioxide) On Colon Cancer Cells: Our First-Step Experience And In Vitro Results, Özgür Albuz, Di̇lek Dülger, Beste Çağdaş Tunali, Feray Aydin, Seli̇m Yalçin, Mustafa Türk

Turkish Journal of Biology

Boron oxide (B2O3) is derived from dehydration of boric acid and is a colorless, semitransparent, crystalline compound that is moderately soluble in water. On the other hand, boron oxide is chemically hygroscopic. This gives the molecule the ability to soak up water and adhere to tissues. Boron oxide can be used locally after tumor debulking in inoperable tumors and especially when the tumor-free margin distance cannot be provided. For all these reasons we aimed to evaluate the in vitro test results of B2O3 in terms of cytotoxicity, genotoxicity, apoptosis, and necrotic effects on L929 fibroblast cells and DLD-1 colorectal adenocarcinoma …

Keratin 14 Is A Novel Interaction Partner Of Keratinocyte Differentiation Regulator:Receptor-Interacting Protein Kinase 4, Ceren Sümer, Asi̇ye Büşra Boz Er, Tuba Di̇nçer

Turkish Journal of Biology

The epidermis, the outer layer of the skin, is formed by stratified keratinocyte layers. The self-renewal of the epidermis is provided by sustained proliferation and differentiation of the keratinocyte stem cells localized to the basal layer of the epidermis. Receptorinteracting protein kinase 4 (RIPK4) is an important regulator of keratinocyte differentiation, mutations of which are associated with congenital ectodermal malformations. In an attempt to identify the molecular basis of RIPK4's function, we applied yeast two-hybrid screen (Y2H) and found basal layer-specific keratin filament component keratin 14 (KRT14) as a novel RIPK4-interacting partner. During keratinocyte differentiation, layer-specific keratin composition is tightly …