Biology Commons^™

Functional Analysis Of Protease Adamts 6 During Vertebrate Eye Formation, Austin Taylor

Lewis Honors College Capstone Collection

Extracellular matrix (ECM) remodeling and epithelial sheet fusion occur during the development of many different tissues, including the vertebrate eye (Lu et al., 2011) (Pai et al., 2012). During morphogenesis of the eye, the ventral hemisphere of the developing retinal tissue forms an opening called the optic fissure which must undergo epithelial fusion (Pai et al., 2012). Failure of optic fissure fusion leads to a congenital blinding disorder called coloboma (Gregory-Evans, 2004). A long standing question pertaining to the mechanism of optic fissure fusion is exactly which ECM remodeling enzymes are actively involved in dismantling the ECM to allow for …

Role Of Glycerol-3-Phosphate Permeases In Plant Defense, Juliana Moreira Soares

Theses and Dissertations--Plant Pathology

Systemic acquired resistance (SAR) is a type of plant defense mechanism that is induced after a localized infection and confers broad-spectrum immunity against related or unrelated pathogens. During SAR, a number of chemical signals and proteins generated at the site of primary infection travel to the uninfected tissues and are thought to alert the distal sites against secondary infections. Glycerol-3-phosphate (G3P) is one of the chemical signals that play an important role in SAR. G3P is synthesized in the cytosol and chloroplasts via the enzymatic activities of G3P Dehydrogenase (G3Pdh) or Glycerol Kinase (GK). Interestingly, a mutation in three of …

The Evolution Of Androgen Receptor Expression And Behavior In Anolis Lizard Forelimb Muscles, Michele A. Johnson, Bonnie K. Kircher, Diego J. Castro

Biology Faculty Research

The motor systems that produce behavioral movements are among the primary targets for the action of steroid hormones, including androgens. Androgens such as testosterone bind to androgen receptors (AR) to induce physiological changes in the size, strength, and energetic capacity of skeletal muscles, which can directly influence the performance of behaviors in which those muscles are used. Because tissues differentially express AR, resulting in tissue-specific sensitivity to androgens, AR expression may be a major target of selection for the evolution of behavior. Anolis lizards (i.e., anoles) provide a robust system for the study of androgen-regulated traits, including the behavioral traits …

Cocaine Selectively Reorganizes Excitatory Inputs To Substantia Nigra Pars Compacta Dopamine Neurons, Gerard M.J. Beaudoin Iii, J. A. Gomez, J. M. Perkins, J. L. Bland, A. K. Petko, C. A. Paladini

Biology Faculty Research

Substantia nigra pars compacta (SNc) dopamine neurons and their targets are involved in addiction and cue-induced relapse. However, afferents onto SNc dopamine neurons themselves appear insensitive to drugs of abuse, such as cocaine, when afferents are collectively stimulated electrically. This contrasts with ventral tegmental area (VTA) dopamine neurons, whose glutamate afferents react robustly to cocaine. We used an optogenetic strategy to isolate identified SNc inputs and determine whether cocaine sensitivity in the mouse SNc circuit is conferred at the level of three glutamate afferents: dorsal raphé nucleus (DR), pedunculopontine nucleus (PPN), and subthalamic nucleus (STN). We found that excitatory afferents …

Repeat Associated Mechanisms Of Genome Evolution And Function Revealed By The Mus Caroli And Mus Pahari Genomes, David Thybert, Maša Roller, Fábio C.P. Navarro, Ian Fiddes, Ian Streeter, Christine Feig, David Martin-Galvez, Mikhail Kolmogorov, Václav Janoušek, Wasiu Akanni, Bronwen Aken, Sarah Aldridge, Varshith Chakrapani, William Chow, Laura Clarke, Carla Cummins, Anthony Doran, Matthew Dunn, Leo Goodstadt, Kerstin Howe, Matthew Howell, Ambre-Aurore Josselin, Robert C. Karn, Christina M. Laukaitis, Lilue Jingtao, Fergal Martin, Matthieu Muffato, Stefanie Nachtweide, Michael A. Quail, Cristina Sisu, Mario Stanke, Klara Stefflova, Cock Van Oosterhout, Frederic Veyrunes, Ben Ward, Fengtang Yang, Golbahar Yazdanifar, Amonida Zadissa, David J. Adams, Alvis Brazma, Mark Gerstein, Benedict Paten, Son Pham, Thomas M. Keane, Duncan T. Odom, Paul Flicek

Scholarship and Professional Work - LAS

Understanding the mechanisms driving lineage-specific evolution in both primates and rodents has been hindered by the lack of sister clades with a similar phylogenetic structure having high-quality genome assemblies. Here, we have created chromosome-level assemblies of the Mus caroli and Mus pahari genomes. Together with the Mus musculus and Rattus norvegicus genomes, this set of rodent genomes is similar in divergence times to the Hominidae (human-chimpanzee-gorilla-orangutan). By comparing the evolutionary dynamics between the Muridae and Hominidae, we identified punctate events of chromosome reshuffling that shaped the ancestral karyotype of Mus musculus and Mus caroli between 3 and 6 million yr …

Shoreline Characterization For Northern Indian River Lagoon And Mosquito Lagoon, Melinda J. Donnelly, Linda Walters, Michelle Shaffer

CEELAB Research Data

The purpose of this project was to evaluate the current conditions of estuarine shorelines in Mosquito Lagoon and northern Indian River. The shoreline assessment is the first phase in the development of a shoreline restoration suitability model for the northernmost section of the Indian River Lagoon system, in order to better conserve and restore shorelines and promote sustainable use of natural resources. The shoreline survey methodology was developed by St. Johns River Water Management for northeastern Florida and was adapted for applications in Brevard and Volusia Counties. Between November 2017 through June 2018, shoreline evaluations were completed at transects located …

Assessing Patterns Of Barn Owl Tyto Alba Occupancy From Call Broadcast Surveys, Tempe Regan, Christopher J.W. Mcclure, James R. Belthoff

Biology Faculty Publications and Presentations

Owing to habitat loss, changes in farming practices, urbanization, and high mortality through vehicle collisions, barn owls Tyto alba are a species of conservation concern in portions of their range. This species can be secretive and difficult to survey, particularly away from breeding sites, so factors related to barn owl occurrence often remain unknown. We conducted nighttime broadcast surveys for barn owls during the early- and post-breeding seasons and used an occupancy modeling framework to understand how factors related to landcover, landscape features, and human development related to occupancy in southern Idaho, USA. We also assessed the effectiveness of using …

Water Availability Influences Arthropod Water Demand, Hydration And Community Composition On Urban Trees, Kevin E. Mccluney, Thomas George, Steven D. Frank

Biological Sciences Faculty Publications

The drive for animals to regulate their water content can have significant consequences for food webs in xeric ecosystems. But the importance of animal water balance (gains vs losses) for mesic food webs has not been explored. Impervious surfaces in cities absorb and re-radiate solar radiation, raising local temperatures. Higher temperatures lead to greater rates of organismal water loss. Thus, urbanization of mesic regions may lead to greater likelihood of desiccation, with consequences for food webs. We tested the effects of animal water balance on a mesic urban food web by supplementing animal-available water (but not plant) within trees in …

Independent Association Of Sudden Cardiac Death And The Tpeak-Tend Interval On Vectorcardiogram, Ryan Richard Gonzales

CUP Undergraduate Research

The causes of Sudden Cardiac Death (SCD) are still uncertain in the field of electro-cardiology. Cardiac repolarization may be the instance in which fatal arrhythmias lead to SCD development. Cardiac disease has not been well treated, as it is the leading cause of death in industrialized nations. Correlation of the T wave length to Sudden Cardiac Death (SCD) has been demonstrated in recent studies, most notably in a meta-analysis published in 2017, (Tse, Gong, Wong, Georgopoulos, Letsas, Vassiliou, … Liu, 2017). However, this project is attempting to show independent association of the T wave and SCD. Independent association demonstrates some …

Measuring Visual Acuity In Awake Mice Using Visually Evoked Potentials (Veps), Emanuel Drutu

CUP Undergraduate Research

We investigated a means to measure visual acuity in awake mice using visually evoked potentials (VEPs). Using counter-phasing sinusoidal gratings as stimuli, we compared the effectiveness of vertical and horizontal orientations in generating VEPs. Using stereotaxic implanted electrodes in the animal’s primary visual cortex, the evoked VEPs were recorded and analyzed. At the lowest spatial frequency, vertical stimuli evoked the largest VEP amplitude. However, at higher spatial frequencies, a reversal occurs where horizontal gratings evoked larger VEPs. These data suggest vertical and horizontal stimuli have different effects on generating VEPs although further investigation is required to determine which stimulus is …

Author Correction: Evolutionary History Of Plant Hosts And Fungal Symbionts Predicts The Strength Of Mycorrhizal Mutualism, Jason D. Hoeksema, James D. Bever, Sounak Chakraborty, V. Bala Chaudhary, Monique Gardes, Catherine A. Gehring, Miranda M. Hart, Elizabeth A. Housworth, Wittaya Kaonongbua, Marc J. Lajeunesse

Integrative Biology Faculty and Staff Publications

In the original published version of the article, the description of the fixed-effect predictor Inoculum Complexity presented in the Methods was incorrect. The incorrect description given was: “single fungal genus, multiple fungal genera, or whole soil inoculum”. The correct description is: “single fungal species, multiple fungal species, or whole soil inoculum”. The error does not affect any of the results presented in the paper. The correction has been made to the HTML and PDF versions of the paper.

Detection Of Local Steroidogenic Enzyme Gene Expression In Brown Anoles, Ada Spahija

Honors Program Theses

The endocrine system in vertebrates responds to stress by releasing steroid hormones, mainly glucocorticoids (GC), which increase blood glucose levels to supply key organs and muscles with energy needed for survival. Steroid hormones are synthesized via an enzymatic pathway that converts cholesterol into either GCs, androgens, or estrogens in a step-wise manner. The adrenal cortex is known to produce GCs, but evidence suggests that individual organs can also produce steroid hormones de novo in response to stress. This study aims to quantify gene expression of four steroidogenic enzymes, encoded by CYP19A1, CYP17A1, StAR, and HSD17ß3 genes, via qRT-PCR to determine …

Nuclear Features Of The Heterotrich Ciliate Blepharisma Americanum: Genomic Amplification, Life Cycle, And Nuclear Inclusion, Megan M. Wancura, Ying Yan, Laura A. Katz, Xyrus X. Maurer-Alcalá

Biological Sciences: Faculty Publications

Blepharisma americanum, a member of the understudied ciliate class Heterotrichea, has a moniliform somatic macronucleus that resembles beads on a string. Blepharisma americanum is distinguishable by its pink coloration derived from the autofluorescent pigment blepharismin and tends to have a single somatic macronucleus with 3–6 nodes and multiple germline micronuclei. We used fluorescence confocal microscopy to explore the DNA content and amplification between the somatic and germline nuclei of B. americanum through its life cycle. We estimate that the DNA content of the macronucleus and micronucleus are 43 ± 8 Gbp and 83 ± 16 Mbp respectively. This correlates with …

A Rapid And Efficient Method For Enriching Mitochondrial Dna From Plants, Mackenzie M. Strehle, Emma Purfeerst, Alan C. Christensen

School of Biological Sciences: Faculty Publications

Current mitochondrial purification techniques are tedious and protracted due to their emphasis on recovering physiologically active mitochondria. However, for studies that are exclusively interested in isolating mitochondrial DNA (mtDNA) for applications such as PCR and sequencing, respiring mitochondria - and the complex procedures that stem from the need to retain their function - are unnecessary. Still, global DNA extraction methods have proven insufficient for mitochondrial DNA isolation because nuclear mitochondrial DNA segments (NUMTs) pose unique challenges to accurate mtDNA quantification and characterization. We present a rapid and simple extraction technique that maximizes recovery of mitochondrial DNA from plant cells, while …

A Closer Look At The Items Within Three Measures Of Evolution Acceptance: Analysis Of The Mate, I-Sea, And Gaene As A Single Corpus Of Items, William L. Romine, Amber Todd, Emily M. Walter

Biological Sciences Faculty Publications

Background

Current direct Likert measures for evolution acceptance include the MATE, GAENE, and I-SEA. Pros and cons of each of these instruments have been debated, and yet there is a dearth of research teasing out their similarities and differences when they are used together in a single context beyond the fact that their measures tend to be highly correlated. We administered these to 452 college students in non-major biology classes at two research-intensive universities from the Midwestern and Western United States to investigate the measurement properties of the items within these instruments when combined as a single corpus.

Results

Factor …

Changes In Water Quality Of Grand Lake St. Marys Watershed Following Implementation Of A Distressed Watershed Rules Package, Stephen J. Jacquemin, Laura T. Johnson, Theresa A. Dirksen, Gregory J. Mcglinch

Biological Sciences Faculty Publications

Grand Lake St. Marys watershed has drawn attention over the past decade as water quality issues resulting from nutrient loading have come to the forefront of public opinion, political concern, and scientific study. The objective of this study was to assess long-term changes in water quality (nutrient and sediment concentrations) following the distressed watershed rules package instituted in 2011. Since that time, a variety of rules (e.g., winter manure ban) and best management practices (cover crops, manure storage or transfers, buffers, etc.) have been implemented. We used a general linear model to assess variation in total suspended solids, particulate phosphorus, …

Impacts Of Temperature On Life History Traits Of Invasive Eastern Brook Trout ( Salvelinus Fontinalis ), Kaeli Alexis Davenport

EWU Masters Thesis Collection

No abstract provided.

Characterizing The Role Of The Cellular Protein Schlafen 11 In Flavivirus Replication, Federico Valdez

Open Access Theses & Dissertations

Schlafen 11 (Slfn11) is a ubiquitously expressed interferon stimulating gene (ISG) that controls synThesis of proteins by regulating tRNA abundance. Through this mechanism Slfn11 has previously been shown to impair human immunodeficiency virus 1 (HIV-1) infection and the expression of codon-biased open reading frames. Because replication of positive-sense single- stranded RNA [(+)ssRNA viruses] requires the immediate translation of the incoming viral genome whereas negative sense, single stranded [(-)ssRNA] viruses carry at infection an RNA replicase that makes multiple translation competent copies of the incoming viral genome, we reasoned that (+)ssRNA viruses will be more sensitive to the effect of Slfn11 …

Chemotaxis Of Batrachochytrium Dendrobatidis Zoospores In Response To Live Amphibians, David Bartholow

Masters Theses

Batrachochytrium dendrobatidis (Bd) is one of the primary drivers of amphibian decline, and has spread rapidly on a global scale, but the mechanisms of Bd movement on small spatial scales are poorly understood and may play a role in transmission and infection. The flagellated zoospores of this fungus exhibit chemotaxis in response to single chemical cues, towards potential nutrient sources and away from metabolites of anti-fungal bacteria (AFB) present on amphibian skin. Levels of cutaneous AFB were manipulated on Eurycea bislineata hosts (either by bathing in a culture of the AFB Janthinobacterium lividum, or bathing in antibiotics) …

Seasonal Fluctuations Of Zooplankton, Dissolved Oxygen, And Temperature In Lake Bemidji, Minnesota, Cody Coyle

Journal of Earth and Life Science

With the current influx of aquatic invasive species in Minnesota lakes, the status of zooplankton has become vital. Zooplankton are at the base of all food chains in aquatic ecosystems, ecosystems that vary greatly throughout a year’s time. The objectives of this study were to 1) track the location of zooplankton throughout the water column for an entire year, and 2) establish relationships between zooplankton location and specific water column characteristics. Zooplankton, dissolved oxygen, and temperature samples were collected bi-monthly at various depths at the deepest point in the southern basin of Lake Bemidji. Zooplankton were most abundant in the …

Age-0 Walleye Growth Rates Following Spiny Water Flea Bythotrephes Longimanus Invasion, Joseph Amundson

Journal of Earth and Life Science

Within Voyageurs National Park, spiny water flea Bythotrephes longimanus has reduced Lake Kabetogama and Rainy Lake native zooplankton biomass during summer peak by 40-60%. Subsequently, planktivorous age-0 Yellow Perch Perca flavescens growth has decreased. A possible trophic cascade may influence predatory fish growth rates like that of age-0 Walleye Sander vitreus. Using seine net and electrofishing data, age-0 Walleye growth rates were modeled as a linear function of growing degree days (GDD) and the slope was compared between pre- and post-spiny water flea establishment. Nearby Lake Vermilion, assumed to have been unaffected by spiny water flea until 2015 and …

Burbot Activity In Relation To Light Penetration On Lake Bemidji, Mn, Hunter Weidenborner, Josh Janecek

Journal of Earth and Life Science

Burbot Lota lota is the only freshwater member of the family Gadidae and has a circumpolar distribution in cold-water regions. Information related to Burbot biology is limited in comparison to most fishes. Snow pack depth and ice conditions influence light penetration and therefore primary production levels. Primary production can have cascading effects on higher trophic levels. Therefore, the objective of this study was to observe if light levels penetrating through cleared and covered ice has an effect on Burbot activity in Lake Bemidji, MN. Angling for Burbot occurred for a minimum of 30 four hour sample periods at both cleared …

Characterizing Biogeochemical Shifts In Two Salix Caroliniana Michx. Encroached Freshwater Subtropical Marshes, Janet Ho

Electronic Theses and Dissertations

Shrub encroachment is a common disturbance in wetlands, but little is known about how shrub encroachment impacts functions such as carbon (C) storage and nitrogen (N) cycling. The objective of this thesis was to identify differences in physiochemical properties (within soil, water and leaf tissue) and biogeochemical processes (soil respiration, enzyme activity, litter decomposition, and N cycling) in two subtropical freshwater marshes encroached by coastal plain willow (Salix caroliniana Michx.). Two study regions (Moccasin Island and Lake Apopka) were selected because of their location in the St. John's River watershed and their unique histories and hydroperiod, allowing for an investigation …

Vertebrate Natural History Notes From Arkansas, 2018, Renn Tumlison, D. Blake Sasse, Henry W. Robison, Matt B. Connior, Chris T. Mcallister, Kelly Jobe, Matthew Anderson

Journal of the Arkansas Academy of Science

Because meaningful observations of natural history are not always part of larger studies, important pieces of information often are unreported. Small details, however, can fill gaps in understanding and lead to interesting questions about ecological relationships or environmental change. We have compiled recent important observations of distribution, deformities, and foods of various vertebrates, observations of winter activity of a woodchuck (Marmota monax) and winter torpor of a hoary bat (Lasiurus cinereus), and also report a very unusual case of bilateral gynandromorphism in a Cardinal (Cardinalis cardinalis). These unique observations continue to add immensely to …

Gene Flow And Genetic Structure Of Two Of Arkansas’S Rarest Darter Species (Teleostei: Percidae), The Arkansas Darter, Etheostoma Cragini, And The Least Darter, E. Microperca, Justin Baker, Brian Wagner, Robert Wood

Journal of the Arkansas Academy of Science

Distinguishing the effects of naturally caused historical fragmentation from those of contemporary landscape modification is critically important to understanding the consequences of human influences on patterns of gene flow and population dynamics. Nonetheless, relatively few recent studies focusing on this issue have dealt with species that showed evidence of historical fragmentation. In the current study, we disentangled the effects of fragmentation operating over separate timescales on two darter species, Etheostoma cragini and E. microperca, from the Ozark Highlands. Formerly more wide-spread within this region in Arkansas, these species now occur only in highly isolated habitats (i.e., spring-runs). …

Distribution Of Dracunculus Sp. Infection In River Otters (Lontra Canadensis) In Arkansas, Renn Tumlison, Allison Surf, Heather May

Journal of the Arkansas Academy of Science

No abstract provided.

Wild Type Drosophila Melanogaster Eye Pigments: Examining Absorbance Spectra And Light Sensitivity, Erin Brown, Taylor M. Delaney

A with Honors Projects

The eyes of wild type fruit flies contain various pigments that contribute to a brick red color. Chromatography techniques are used to separate and view these pigments. IN this project, the students extract and gather absorbance spectra for these pigments using three methods: eye pigment chromatography, pigment extraction and spectrophotometry, and spectrophotometry and photosensitivity. The methods are compared to determine how efficient they are at separating and extracting pigments and producing reliable results when examining absorbance spectra. To explore the photosensitivity of the pigments, we compare absorbance spectra changes to a pigment solution.

Rna-Seq Of Kaposi's Sarcoma Reveals Alterations In Glucose And Lipid Metabolism, For Yue Tso, Andrew V. Kossenkov, Salum J. Lidenge, Owen Ngalamika, John R. Ngowi, Julius Mwaiselage, Jayamanna Wickramasinghe, Eun Hee Kwon, John T. West, Paul M. Lieberman, Charles Wood

School of Biological Sciences: Faculty Publications

Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent of Kaposi's sarcoma (KS). It is endemic in a number of sub-Saharan African countries with infection rate of >50%. The high prevalence of HIV-1 coupled with late presentation of advanced cancer staging make KS the leading cancer in the region with poor prognosis and high mortality. Disease markers and cellular functions associated with KS tumorigenesis remain ill-defined. Several studies have attempted to investigate changes of the gene profile with in vitro infection of monoculture models, which are not likely to reflect the cellular complexity of the in vivo lesion environment. Our approach …

The Contributions Of Fc Gamma Receptors And Macropinocytosis To The Internalization, Sorting And Clearance Of Antibody Coated Nanovesicles In Macrophages, George Opoku-Kusi Jr.

Electronic Theses and Dissertations

Macrophages are tissue-resident phagocytes that play critical roles in immune response and tissue homeostasis. They have a tremendous capacity to internalize objects of various sizes ranging from nanoscale viral particles to micron sized bacteria and tumor cells. This phenomenon, termed phagocytosis for the uptake of large particles, or endocytosis for the uptake of small particles, is integral to the immune response in multicellular organisms. Macrophages express FcγRs, which are tyrosine kinase receptors that bind IgG on an opsonized target. Binding of IgG Fc domain to the extracellular domain of an FcγR triggers signaling cascades that coordinate internalization of the opsonized …

Evaluation Of Dietary Phytochemicals As Rumen Modifiers In Lactating Dairy Cows, Sonia Rodriguez Jimenez

Electronic Theses and Dissertations

Phytochemicals such as tannins included in dairy cow nutritional programs, as a natural feed additive, promise to improve rumen bypass protein and consequently reduce the feeding cost by decreasing the amount of CP needed in the ration. Additionally, if tannins are fed along with high quality protein sources, this could improve the AA profile reaching the small intestine and potentially improving health and performance of dairy cows. The black pepper, specifically its alkaloid piperine, is a nontoxic, natural dietary compound with a broad range of physiological activity. Lactating dairy cows ingesting these phytochemicals (tannin extracts and black pepper) will potentially …