Biology Commons^™

Increased Insertion Number Leads To Increased Sperm Transfer And Fertilization Success In A Nursery Web Spider, Alissa G. Anderson, Eileen Hebets

Eileen Hebets Publications

Across animals, a male's fitness is largely dictated by his ability to fertilize eggs; and there exists a plethora of male adaptations associated with increasing fertilization success. In the nursery web spider, Pisaurina mira, males restrain females prior to and during copulation by wrapping them with silk. Previous research demonstrates that copulatory silk wrapping reduces a male's chance of being sexually cannibalized and increases the number of sperm transfer opportunities (termed insertions) that a male can achieve within a mating. While avoiding cannibalism provides an obvious survival benefit to males, the impact of insertion number on male fitness remains …

Importance Of The Antenniform Legs, But Not Vision, For Homing By The Neotropical Whip Spider Paraphrynus Laevifrons, Verner P. Bingman, Jacob M. Graving, Eileen Hebets, Daniel D. Wiegmann

Eileen Hebets Publications

Amblypygids, or whip spiders, are nocturnal, predatory arthropods that display a robust ability to navigate to their home refuge. Prior field observations and displacement studies in amblypygids demonstrated an ability to home from distances as far away as 10 m. In the current study, micro-transmitters were used to take morning position fixes of individual Paraphrynus laevifrons following an experimental displacement of 10 m from their home refuge. The intention was to assess the relative importance of vision compared with sensory input acquired from the antenniform legs for navigation as well as other aspects of their spatial behavior. Displaced individuals were …

Measurement Instrument For Scientific Teaching (Mist): A Tool To Measure The Frequencies Of Research-Based Teaching Practices In Undergraduate Science Courses, Mary F. Durham, Jennifer K. Knight, Brian A. Couch

School of Biological Sciences: Faculty Publications

The Scientific Teaching (ST) pedagogical framework provides various approaches for science instructors to teach in a way that more closely emulates how science is practiced by actively and inclusively engaging students in their own learning and by making instructional decisions based on student performance data. Fully understanding the impact of ST requires having mechanisms to quantify its implementation. While many useful instruments exist to document teaching practices, these instruments only partially align with the range of practices specified by ST, as described in a recently published taxonomy. Here, we describe the development, validation, and implementation of the Measurement Instrument for …

Student Buy-In Toward Formative Assessments: The Influence Of Student Factors And Importance For Course Success, Kathleen R. Brazeal, Brian A. Couch

School of Biological Sciences: Faculty Publications

Formative assessment (FA) techniques, such as pre-class assignments, in-class activities, and post-class homework, have been shown to improve student learning. While many students find these techniques beneficial, some students may not understand how they support learning or may resist their implementation. Improving our understanding of FA buy-in has important implications, since buy-in can potentially affect whether students fully engage with and learn from FAs. We investigated FAs in 12 undergraduate biology courses to understand which student characteristics influenced buy-in toward FAs and whether FA buy-in predicted course success. We administered a mid-semester survey that probed student perceptions toward several different …

Sigma Virus (Dmelsv) Incidence In Lines Of Drosophila Melanogaster Selected For Survival Following Infection With Bacillus Cereus, Meghan L. Bentz, Eve A. Humphrey, Lawrence G. Harshman

School of Biological Sciences: Faculty Publications

The immune response of Drosophila melanogaster is complex and involves both specific and general responses to parasites. In this study we tested for cross-immunity for bacteria and viruses by scoring the incidence of infection with the vertically transmitted Sigma virus (DMelSV) in the progeny of a cross between females transmitting DMelSV at high frequencies and males from lines subjected to three selection regimes related to resistance to Bacillus cereus. There was no significant difference in transmission of DMelSV among selection regimes, though results suggest that the B. cereus selected lines had lower rates of infection by DMelSV. We found …

Mucking About: Hydrologic Regime And Soil Carbon Storage In Restored Subtropical Wetlands, Alicia Huber

Electronic Theses and Dissertations

Wetlands are extremely important ecosystems that have declined drastically worldwide, continue to be lost, and are threatened globally. They perform a number of important ecosystem services such as flood control, provide habitat for many species, and have aesthetic and recreational value. Wetlands are also important to the global carbon (C) cycle. Wetland soils are especially effective C sinks because they have high primary productivity and low decomposition rates due to flooded, anoxic conditions. Increased recognition of wetlands' value has led to more ecological and hydrological restoration of degraded wetlands to mitigate the effects of wetland destruction. Hydrological restoration, which attempts …

Quorum Quenching Activity Of Lysates From Bioluminescent Bacteria Isolated From Selected Philippine Marine And Freshwater Organisms, Janice A. Ragaza, Roja G. Angeles-Gumban, Mudjekeewis D. Santos, Minerva Fatimae H. Ventolero, Ronie J. Calugay

Biology Faculty Publications

Pathogens use cell density dependent gene expression known as Quorum Sensing (QS) to launch virulent factors. Targeting the QS circuit without killing the pathogens is a very promising alternative to using antibiotics which generate antibiotic resistance. The identity and diversity of bioluminescent bacteria associated with Philippine aquatic organisms, and their potential as sources of anti-QS or quorum quenching (QQ) molecules remain unknown. In this study, out of 43 organisms, 30 were observed to harbor bioluminescent bacteria with 26 from marine and four from freshwater sources. Purified bioluminescent bacterial cultures were screened for QQ activity using motility assay and the membrane …

Analysis Of The Oil Biosynthesis Transcripts Of The Moringa Oleifera Lam. Mature Seed Embryos Using Rna Sequencing, Vivian A. Panes, A Kitazumi, M Butler, R.D Baoas, B.G De Los Reyes

Biology Faculty Publications

Moringa oleifera seeds are capable of producing 40% edible oils that are gaining significance due to its nutritional advantages. Several studies have examined M. oleifera seed oil, nevertheless, these studies focused on the extraction of oil and methods of biodiesel production. There is a paucity of information on transcriptome level studies to determine the unigenes involved in oil biosynthesis metabolic pathways. The main objective of this study is to explore the transcriptome of the mature embryo of M. oleifera Lam. particularly the key genes related to oil biosynthesis. The transcriptome reflects the set of genes that are actively expressed at …

Plasticity Of Central And Peripheral Nervous System: Effects Of Oxygen-Glucose Deprivation (Ogd) And Chronic Intermittent Hypoxia (Cih), Jin Chen

Electronic Theses and Dissertations

Transient receptor potential canonical 6 (TRPC6) channels are permeable to Na+ and Ca2+ and are widely expressed in the brain. In this study, we investigated the role of TRPC6 following ischemia/reperfusion (I/R) and oxygen-glucose deprivation (OGD). We found that TRPC6 expression was increased in wild type (WT) mice cortical neurons following I/R and in primary neurons with OGD, and that deletion of TRPC6 reduced the I/R-induced brain infarct in mice and the OGD- /neurotoxin-induced neuronal death. Using live-cell imaging to examine intracellular Ca2+ levels ([Ca2+]i), we found that OGD induced a significant higher increase in glutamate-evoked Ca2+ influx compared to …

An Rnai Screen To Identify Components Of A Polyamine Transport System, Adam J. Foley

Honors Undergraduate Theses

Polyamines, specifically putrescine, spermidine, and spermine, are small cationic molecules found in all organisms. Cells can biosynthetically make these molecules, or alternatively, they can be transported from the extracellular environment. Malignant cells have been shown to require relatively high amounts of polyamines. There is a chemotherapeutic agent, DFMO, used to block the biosynthesis of polyamines. Many malignant cells can circumvent DFMO therapy by activating their transport system. A potential solution is to simultaneously block biosynthesis and transport of polyamines. However, little is known about the polyamine transport system in higher eukaryotes.

This thesis aims to add to the basic biological …

Changing Measurements Or Changing Movements? Sampling Scale And Movement Model Identifiability Across Generations Of Biologging Technology, Leah R. Johnson, Philipp H. Boersch-Supan, Richard A. Phillips, Sadie J. Ryan

Integrative Biology Faculty and Staff Publications

Animal movement patterns contribute to our understanding of variation in breeding success and survival of individuals, and the implications for population dynamics. Over time, sensor technology for measuring movement patterns has improved. Although older technologies may be rendered obsolete, the existing data are still valuable, especially if new and old data can be compared to test whether a behavior has changed over time. We used simulated data to assess the ability to quantify and correctly identify patterns of seabird flight lengths under observational regimes used in successive generations of wet/dry logging technology. Care must be taken when comparing data collected …

Genetic And Epigenetic Variation In Spartina Alterniflora Following The Deepwater Horizon Oil Spill, Marta Robertson, Aaron Schrey, Ashley Shayter, Christina J. Moss, Christina Richards

Integrative Biology Faculty and Staff Publications

Catastrophic events offer unique opportunities to study rapid population response to stress in natural settings. In concert with genetic variation, epigenetic mechanisms may allow populations to persist through severe environmental challenges. In 2010, the Deepwater Horizon oil spill devastated large portions of the coastline along the Gulf of Mexico. However, the foundational salt marsh grass, Spartina alterniflora, showed high resilience to this strong environmental disturbance. Following the spill, we simultaneously examined the genetic and epigenetic structure of recovering populations of S. alterniflora to oil exposure. We quantified genetic and DNA methylation variation using amplified fragment length polymorphism and methylation …

Microbe Biogeography Tracks Water Masses In A Dynamic Oceanic Frontal System, Anni Djurhuus, Philipp H. Boersch-Supan, Svein-Ole Mikalse, Alex D. Rogers

Integrative Biology Faculty and Staff Publications

Dispersal limitation, not just environmental selection, plays an important role in microbial biogeography. The distance–decay relationship is thought to be weak in habitats where dispersal is high, such as in the pelagic environment, where ocean currents facilitate microbial dispersal. Most studies of microbial community composition to date have observed little geographical heterogeneity on a regional scale (100 km). We present a study of microbial communities across a dynamic frontal zone in the southwest Indian Ocean and investigate the spatial structure of the microbes with respect to the different water masses separated by these fronts. We collected 153 samples of free-living …

Behavioral And Vocal Responses Of The American Goldfinch (Carduelis Tristis) To Predatory Bird Calls, Kate Marsh

Senior Honors Theses and Projects

A particular reaction to a given predator is dependent on the level of danger perceived by an animal. American Goldfinches (Carduelis tristis) are preyed upon by highly threatening predators that commonly attack songbirds at feeders (feeder predator) and less threatening predators that rarely attack songbirds at feeders (non-feeder predators). This study measured the behavioral and vocal reactions of American goldfinches to these different levels of predatory threat and examined the effect of threat level on the trade-off between foraging and anti-predatory behaviors (vigilance). We hypothesized that if goldfinches respond to predatory cues based on the level of threat …

The Effect Of Temporary Anosmia On Olfactory Bulb Glomerular Neuron Activation, Kelsey E. Zuk

Senior Honors Theses and Projects

Neuroplasticity refers to changes in signaling and circuitry throughout the nervous system. The olfactory system is well known for its ability to induce neuroplasticity throughout the lifespan of the organism. Changes in olfactory sensory input are known to induce neuroplasticity. Neuron activation, due to olfactory sensory input, activates the immediate early gene c-fos in the olfactory bulb (OB). The presence of fos, the protein derived from c-fos gene expression, can be used to measure changes in OB circuit activity. In the present study, olfactory sensory input was disrupted in mice by intranasal irrigation with the detergent Triton X-100. This induces …

Effect Of Cd40 Silencing In Mav-1 Infected Mh-S Cells, Andre Obua

Senior Honors Theses and Projects

The murine alveolar macrophage cell line, MH-S, constitutively expresses the immunoregulatory antigen CD40. Previous experiments have demonstrated that CD40 expression is upregulated following infection with the DNA virus, mouse adenovirus 1 1 Obua (MA V-1 ), suggesting a role for CD40 in MA V-1 infection. The purpose of this experiment is to investigate the effect ofCD40 silencing on MA V-1 replication in MH-S cells. To elucidate the importance ofCD40 signaling in MA V-1 replication, CD40 expression was reduced using a short hairpin RNA (shRNA) RNAi system. Five CD40-silenced lines were generated using shRNA and showed between 26-79 percent reduction in …

The Effects Of Unpredictable Food On Corticosterone And Social Learning In Red Crossbills (Loxia Curvirostra), Megan Catharine Wurtz

Senior Honors Theses and Projects

Foraging success can limit fitness if resources are unpredictable and strongly favor those that learn socially. Unpredictability can be a stressor and trigger elevations of stress hormones, that coordinate whole body responses to challenging environmental conditions. We explored the effects of unpredictable food on behavior, physiology, and receptiveness to social information using an observer/demonstrator paradigm in red crossbills, a highly social species that are adapted to cope with high degrees of resource unpredictability. Unpredictable food limited food intake (X²₁ = 10, p = 0.0012), caused a decline in body condition (X²₁ = 5.9, p …

Variation In Arthropod Hydration Across Us Cities With Distinct Climate, Kevin E. Mccluney, Justin D. Burdine, Steven D. Frank

Biological Sciences Faculty Publications

Increasing evidence suggests that altered environmental conditions within cities (e.g. temperature) can have strong effects on the biology and ecology of animals. Moreover, multiple abiotic (e.g. soil moisture, distributions of water bodies) and biotic factors (e.g. plant and animal communities) may be more similar among cities in distinct climate regions as compared to outside cities. But the mechanisms behind these patterns are unclear. We suggest that animal physiological condition responds to urbanization and may become more similar with urbanization among cities. As a first attempt at assessing this possibility, we measured hydration (water content) of field-collected arthropods, across variation in …

Invertebrate Neurophysiology: Testing The Efficacy Of Natural Insecticides, Anastasiya S. Ananenka, Wayne Tschetter

CUP Undergraduate Research

Insecticides are widely used in agriculture. While there are several classes of synthetic insecticides that are highly effective against insects, there is data suggesting that these compounds are also toxic to non-target species. However, compounds like caffeine have been shown to act as natural insecticides. Here we investigate the effects of a wellknown class of insecticides, pyrethroids, as well as natural compounds such as caffeine. Since insecticides work at the level of the nervous system we propose to measure neural activity in crickets (Acheta Domestica) after exposure to synthetic and naturally occurring insecticides. Here we show our approach and preliminary …

Suitability Of Laurentian Great Lakes For Invasive Species Based On Global Species Distribution Models And Local Habitat, Andrew M. Kramer, Gust Annis, Marion E. Wittmann, William L. Chadderton, Edward S. Rutherford, David M. Lodge, Lacey Mason, Dmitry Beletsky, Catherine Riseng, John M. Drake

Integrative Biology Faculty and Staff Publications

Efficient management and prevention of species invasions requires accurate prediction of where species of concern can arrive and persist. Species distribution models provide one way to identify potentially suitable habitat by developing the relationship between climate variables and species occurrence data. However, these models when applied to freshwater invasions are complicated by two factors. The first is that the range expansions that typically occur as part of the invasion process violate standard species distribution model assumptions of data stationarity. Second, predicting potential range of freshwater aquatic species is complicated by the reliance on terrestrial climate measurements to develop occurrence relationships …

Accuracy Of Climate-Based Forecasts Of Pathogen Spread, Annakate M. Schatz, Andrew M. Kramer, John M. Drake

Integrative Biology Faculty and Staff Publications

Species distribution models (SDMs) are a tool for predicting the eventual geographical range of an emerging pathogen. Most SDMs, however, rely on an assumption of equilibrium with the environment, which an emerging pathogen, by definition, has not reached. To determine if some SDM approaches work better than others for modelling the spread of emerging, non-equilibrium pathogens, we studied time-sensitive predictive performance of SDMs for Batrachochytrium dendrobatidis, a devastating infectious fungus of amphibians, using multiple methods trained on time-incremented subsets of the available data. We split our data into timeline-based training and testing sets, and evaluated models on each set …

Record Breaking Achievements By Spiders And The Scientists Who Study Them, Stefano Mammola, Peter Michalik, Eileen Hebets, Marco Isaia

Eileen Hebets Publications

Organismal biology has been steadily losing fashion in both formal education and scientific research. Simultaneous with this is an observable decrease in the connection between humans, their environment, and the organisms with which they share the planet. Nonetheless, we propose that organismal biology can facilitate scientific observation, discovery, research, and engagement, especially when the organisms of focus are ubiquitous and charismatic animals such as spiders. Despite being often feared, spiders are mysterious and intriguing, offering a useful foundation for the effective teaching and learning of scientific concepts and processes. In order to provide an entryway for teachers and students—as well …

What Induces Female Kicking In Callosobruchus Maculatus? Disentangling The Effects Of Male Traits On Female Mating Decisions, William I. Licht

Theses and Dissertations--Entomology

Sexual conflict over mating duration drives the evolution of male and female adaptations that facilitate the manipulation of mating interactions in their favor. This conflict drives the evolution of traits that improve the fitness of the focal sex despite inflicting costs on mates. However, males can express multiple traits that increase and decrease female fitness simultaneously. When the effects of male traits on female fitness increase or decrease with duration of exposure, females traits that influence mating duration are selected upon. Females of Callosobruchus maculatus, a bruchid bean beetle, kick mates to forcefully end copulation. Although both negative effects …

First Definitive Fossil Of An Oestroid Fly (Diptera: Calyptratae: Oestroidea) And The Dating Of Oestroid Divergences, Pierfilippo Cerretti, John O. Stireman Iii, Thomas Pape, James E. O'Hara, Marco A. Marinho, Knut Rognes, David A. Grimaldi

Biological Sciences Faculty Publications

Calyptrate flies include about 22,000 extant species currently classified into Hippoboscoidea (tsetse, louse, and bat flies), the muscoid grade (house flies and relatives) and the Oestroidea (blow flies, bot flies, flesh flies, and relatives). Calyptrates are abundant in nearly all terrestrial ecosystems, often playing key roles as decomposers, parasites, parasitoids, vectors of pathogens, and pollinators. For oestroids, the most diverse group within calyptrates, definitive fossils have been lacking. The first unambiguous fossil of Oestroidea is described based on a specimen discovered in amber from the Dominican Republic. The specimen was identified through digital dissection by CT scans, which provided morphological …

A New Tachinid Genus And Species Record For North America: Iceliopsis Borgmeieri Guimarães, John O. Stireman Iii, Jane E. Dell

Biological Sciences Faculty Publications

The Iceliini are a small, enigmatic New World tribe of Tachininae consisting of three genera, Icelia RobineauDesvoidy, Iceliopsis Guimarães, and Erviopsis Townsend, and five recognized species (Guimarães 1976). All known species are exclusively Neotropical in distribution with the exception of Icelia triquetra (Olivier), which ranges from Brazil, through Central America, and as far north as New York state (O’Hara & Wood 2004). Members of the tribe are generally medium-sized (ca. 7–12 mm), elongate, yellowish or grayish in color, and resemble Dexiini or Leskiini in general appearance. Species of Iceliini are relatively rarely collected and there is but a single host …

Intercellular Adhesion Molecule-1 Augments Myoblast Adhesion And Fusion Through Homophilic Trans-Interactions, Francis X. Pizza, Ryan A. Martin, Evan M. Springer, Maxwell S. Leffler, Bryce R. Woelmer, Issac J. Recker, Douglas W. Leaman

Biological Sciences Faculty Publications

The overall objective of the study was to identify mechanisms through which intercellular adhesion molecule-1 (ICAM-1) augments the adhesive and fusogenic properties of myogenic cells. Hypotheses were tested using cultured myoblasts and fibroblasts, which do not constitutively express ICAM-1, and myoblasts and fibroblasts forced to express full length ICAM-1 or a truncated form lacking the cytoplasmic domain of ICAM-1. ICAM-1 mediated myoblast adhesion and fusion were quantified using novel assays and cell mixing experiments. We report that ICAM-1 augments myoblast adhesion to myoblasts and myotubes through homophilic trans-interactions. Such adhesive interactions enhanced levels of active Rac in adherent and …

Genetic Analysis Of Serf Gene Function In Drosophila Melanogaster And Its Contribution To A Fly Model Of Spinal Muscular Atrophy, Swagata Ghosh

Theses and Dissertations--Biology

The Serf gene is evolutionarily highly conserved but its biological function is not known in any organism. In human, SERF1/H4F5 was first identified as a modifier of the disease Spinal Muscular Atrophy (SMA). SMA is caused by mutations in the Survival Motor Neuron 1(SMN1) gene leading to diminished levels of the Smn protein. More than 90% of patients with the most severe form of SMA have deletions that remove SERF1 as well as mutaions within SMN1. Hence, loss of Serf activity is hypothesized to exacerbate SMA disease progression. The primary motivation of this thesis was to test …

Impact Of Prophylactic Intranasal Oxytocin Administration On Symptoms Of Post-Traumatic Stress, Morgan A. Thomas

EWU Masters Thesis Collection

Post-traumatic stress disorder (PTSD) is a mental health condition that affects people after instances of severe emotional trauma. Research suggests that oxytocin treatment decreases PTSD symptoms. This study served to evaluate the efficacy of intranasal oxytocin pre-treatment on symptoms related to PTSD. The hypotheses are that oxytocin will decrease fear and anxiety, and increase reward-seeking behaviors. Sprague Dawley rats were assigned to three groups (Control, Stress, Oxytocin, and Oxytocin+Stress; n=6 per group) to conduct this experiment. Prior to foot shock treatment, rats were trained to expect a food reward (Kellogg’s Froot Loops) in an open field enclosure. Subsequently, the Oxytocin …

Acoustic Tracking Of Redband Rainbow Trout (Oncorhynchus Myskiss Gairdneri) In Lake Roosevelt (2015-2016), Evaluation Of An Acoustic Receiver Array, And Low-Voltage Electroanesthesia For Tag Implantation., Bryan T. Witte

EWU Masters Thesis Collection

Chapter 1. Telemetry methods are able to acquire knowledge of fish movements which are used for a variety of management decisions. Implanting tags for telemetry requires minor surgery and anesthesia. Anesthesia for tag implantation is typically achieved with chemicals. However, chemicals are inherently variable and have restrictions on their use. This has led to the use of alternative methods for achieving anesthesia such as low voltage electroanesthesia (LVEA). LVEA subjects fish to a continuous non-pulsed direct current to achieve anesthesia appropriate for tag implantation. Unlike chemicals fish are quickly anesthetized and recover from anesthesia instantaneously. My objectives were to subject …

Can Amphipods Be Used To Monitor Mining-Impacted Lakes?, Chantilly S. Higbee

EWU Masters Thesis Collection

Metal pollution in aquatic systems is complicated and expensive to manage; establishing a sentinel species for contaminated habitats, rather than measuring abiotic parameters, can provide a more complete perspective of the impacts of pollutants. I conducted a dual field/laboratory study to determine whether an amphipod (Hyalella azteca) can serve as a sentinel in a mining-impacted area in Northern Idaho, the Coeur d’Alene (CDA) River Basin. My objectives in the field study were to (1) determine seasonal abundance and metal burden of amphipods in nine chain lakes and a reference lake (Benewah L.) and (2) compare aqueous trace metal levels and …