Biology Commons^™

Seasonal Changes In Physical, Chemical, And Biotic Factors In Silver Lake, Ohio, Jacob J. Clancy

Honors Theses

The chemical makeup of a body of water can vary greatly depending on what kind of lake it is, the time of year and what kind of runoff enters the water. There are many abiotic factors that make up the water chemistry of a lake such as nutrient availability (Nitrogen and Phosphorous), pH, temperature, oxygen content and conductivity. Each of these factors plays important roles in the successes of many organisms that reside in the lake. The many species of zooplankton and phytoplankton thrive in different water chemistry conditions. Silver Lake is a unique lake in Ohio, because it was …

Does Riparian Forest Invasion By The Exotic Shrub Amur Honeysuckle Influence Nutrient Dynamics In Headwater Streams?, Charlotte Anne Shade

Honors Theses

Lonicera maackii (Amur honeysuckle) invasion is extensive in forests across much of Ohio and the Midwest. Amur honeysuckle has been shown to influence headwater streams and its organisms, which depend on a certain water chemistry to survive. Little has been done to understand how honeysuckle affects water chemistry and nutrient cycling. As honeysuckle canopies prevent native organic matter from entering the streams below, while also adding its own organic matter that is high in nitrogen and phosphorus, and low in lignin, the amount and types of nutrients present in both forests and streams may be significantly altered. Over a one-year …

Use Of A Novel Oleaginous Microorganism As A Potential Source Of Lipids For Weanling Pigs, Jeffery A. Carroll, Ty B. Schmidt, T.R. Callaway, J.G. Wilson, Janet R. Donaldson

Faculty Publications

Weanling pigs are at risk of succumbing to illness due to an immature immune system and insufficient supply of available energy at the time of weaning. This study was aimed at determining whether oleaginous bacteria could serve as a source of lipids to weanling pigs. Weanling pigs were provided a daily dose of 1×109 colony fomring unit (CFU) = kg−1 of the novel oleaginous Enterobacter cloacae strain JD6301 or JD8715 (which is a variant form of JD6301 capable of producing extracellular triglycerides) via oral gavage for 5 d. Serum was collected every 6 h and intestinal samples were collected at …

The Arabidopsis Kinesin-4, Fra1, Requires A High Level Of Processive Motility To Function Correctly, Anindya Ganguly, Logan Demott, Ram Dixit

Biology Faculty Research

Processivity is important for kinesins that mediate intracellular transport. Structure–function analyses of N-terminal kinesins (i.e. kinesins comprising their motor domains at the N-terminus) have identified several non-motor regions that affect processivity in vitro. However, whether these structural elements affect kinesin processivity and function in vivo is not known. Here, we used an Arabidopsis thaliana kinesin-4, called Fragile Fiber 1 (FRA1, also known as KIN4A), which is thought to mediate vesicle transport, to test whether mutations that alter processivity in vitro lead to similar changes in behavior in vivo and whether processivity is important for the function of FRA1. We …

Bacterial Diversity Of An Abandoned Mine Land Soil In Southeast Kansas, Rachel Bechtold

Paper and Posters Presentations

Acid mine drainage (AMD) is found near abandoned coal mines in southeast Kansas as a result of mine waste runoff resulting in low pH. Soil bacteria may be used as an indicator of ecosystem health in these human-perturbed areas. The goal of the present study was to assess the microbial diversity of an AMD site. In fall (2015) and summer (2016), soil samples were aseptically collected from five distant sites representing diverse topography. Soil texture and physico-chemical characters were evaluated. A total of 58 morphologically different colonies were characterized using physiological and biochemical tests and were identified at the species …

Functional Analysis Of Phasirna In Soybean Anther, Hannah Thomas

Paper and Posters Presentations

Many phasiRNA have been discovered but the targets and functions of these molecules remains largely unknown. The function of phasiRNA produced by miR4392, which are expressed highly in the anthers of soybeans were compared to similar ones found in maize. A short tandem target mimic (STTM) was designed to knock out the production of phasiRNA at different points of biogenesis. The resulting constructs were examined for varying phenotypes and molecular variations through RT-qPCR and sRNA library sequencing. Constructs where phasiRNA production was halted developed fewer beans. Failure to produce phasiRNAs in reproductive tissues can lead to important crop yield changes …

Phylogenetic Studies Of The Madagascan Freshwater Crabs (Potamoidea, Potamonautidae, Deckeniinae), Rainee Stevens

All NMU Master's Theses

Relationships within the Madagascan freshwater crab fauna were examined based on unstudied specimens from all parts of Madagascar. This study allowed the examination of the validity of existing genera and species, and identified potential new taxa. The new specimens provided phylogenetic and evolutionary data, as well as new insights into the distribution patterns of the Malagasy freshwater crab fauna. In addition, the large number of new localities from previously unsurveyed areas of Madagascar allowed the construction of updated distribution maps.

The present analysis included 62 unidentified specimens plus 13 identified species of Malagasy freshwater crab taxa that had already been …

Reductions In Mesolimbic Dopamine Signaling And Aversion: Implications For Relapse And Learned Avoidance, Mykel A. Robble

Dissertations (1934 -)

The ability to adjust behavior appropriately following an aversive experience is essential for survival, yet variability in this process contributes to a wide range of disorders, including drug addiction. It is clear that proper approach and avoidance is regulated, in part, by the activity of the mesolimbic dopamine system. While the importance of this system as a critical modulator of reward learning has been extensively characterized, its involvement in directing aversion-related behaviors and learning is still poorly understood. Recent studies have revealed that aversive stimuli and their predictors cause rapid reductions in nucleus accumbens (NAc) dopamine concentrations. Furthermore, a normally …

Each To Their Own Cure: Faculty Who Teach Course-Based Undergraduate Research Experiences Report Why You Too Should Teach A Cure, Erin E. Shortlidge, Gita Bangera, Sara Brownell

Biology Faculty Publications and Presentations

Course-based undergraduate research experiences (CUREs) meet national recommendations for integrating research experiences into life science curricula. As such, CUREs have grown in popularity and many research studies have focused on student outcomes from CUREs. Institutional change literature highlights that understanding faculty is also key to new pedagogies succeeding. To begin to understand faculty perspectives on CUREs, we conducted semi-structured interviews with 61 faculty who teach CUREs regarding why they teach CUREs, what the outcomes are, and how they would discuss a CURE with a colleague. Using grounded theory, participant responses were coded and categorized as tangible or intangible, related to …

The Incidence Of Mrsa Infections In The United States: Is A More Comprehensive Tracking System Needed?, Kevin T. Kavanagh, Said Abusalem, Lindsay E. Calderon

EKU Faculty and Staff Scholarship

A review of epidemiological studies on the incidence of MRSA infections overtime was performed along with an analysis of data available for download from Hospital Compare (https://data.medicare.gov/data/hospital-compare). We found the estimations of the incidence of MRSA infections varied widely depending upon the type of population studied, the types of infections captured and in the definitions and terminology used to describe the results. We could not find definitive evidence that the incidence of MRSA infections in U.S. community or facilities is decreasing significantly. Of concern are recent data reported to the National Healthcare Safety Network (NHSN) on MRSA bloodstream infections which …

Ignatian Pedagogy Certificate Final Project, G. Patrick Duffie

Ignatian Pedagogy Educational Resources

No abstract provided.

Differential Response Of Coral Symbiotic Dinoflagellates To Bacterial Toxins That Produce Bleaching In Stony Corals, Diego L. Gil-Agudelo, Yael Ban-Haim, Eugene Rosenberg, Garriet W. Smith

Journal of the South Carolina Academy of Science

Bleaching of corals and other organisms with symbiotic zooxanthellae is a worldwide phenomenon with increasing importance due to global warming scenarios. Bleaching has been historically related to changes in the environment, especially water temperature increase, that stress corals and provoke the release of zooxanthellae. The discovery of Vibrio shilonii, a bacterium causing bleaching under thermal stress in corals of the Mediterranean Sea has changed our thinking about the cause (or explanation) for bleaching of corals worldwide. During this study, we evaluated the effect of a proline rich toxin, extracted from Vibrio shilonii, on zooxanthellae obtained from: Oculina patagonica from the …

Editorial: Genetics, Genomics And –Omics Of Thermophiles, Kian Mau Goh, Kok-Gan Chan, Rajesh Kumar Sani, Edgardo Ruben Donati, Anna-Louise Reysenbach

Biology Faculty Publications and Presentations

Thermophilic Archaea and Bacteria occupy heated environments. Advancement of next-generation sequencing (NGS), single-cell analyses, and combinations of –omics and microscopic technologies have resulted in the discovery of new thermophiles. This e-book consists of a review, and 10 original articles authored by 94 authors. The main aim of this Research Topic of Frontiers in Microbiology was to provide a platform for researchers to describe recent findings on the ecology of thermophiles using NGS, functional genomics, comparative genomics, gene evolution, and extremozyme discovery.

Rolling Circle Mutagenesis Of Gst-Mcherry To Understand Mutation, Gene Expression, And Regulation, Jessica Cole, Amanda Ferguson, Verónica A. Segarra, Susan Walsh

Biology Faculty Publications and Presentations

Undergraduates are often familiar with textbook examples of human mutations that affect coding regions and the subsequent disorders, but they may struggle with understanding the implications of mutations in the regulatory regions of genes. We have designed a laboratory sequence that will allow students to explore the effect random mutagenesis can have on protein function, expression, and ultimately phenotype. Students design and perform a safe and time-efficient random mutagenesis experiment using error-prone rolling circular amplification of a plasmid expressing the inducible fusion protein glutathione S-transferase (GST)-mCherry. Mutagenized and wild-type control plasmid DNA, respectively, are then purified and transformed into bacteria …

School Of Biological Sciences News, 2017, School Of Biological Sciences

School of Biological Sciences News

Alumni newsletter of Illinois State University's School of Biological Sciences.

Crassulacean Acid Metabolism As A Continuous Trait: Variability In The Contribution Of Crassulacean Acid Metabolism (Cam) In Populations Of Portulacaria Afra, Lonnie J. Guralnick, Kate Gladsky

Arts & Sciences Faculty Publications

Portulacaria afra L. is a dominant facultative CAM species growing in the Southeastern Cape of South Africa. P. afra is well adapted to regions of the Spekboom thicket in areas of limited and sporadic rainfall. P. afra populations occur in isolated drainages. We hypothesized the utilization of CAM would vary in the different populations in response to rainfall and temperature gradients. Carbon isotope composition can be used to determine the contribution of CAM in leaf tissue. P. afra leaves of populations were analyzed in transects running south to north and east to west in locations from the coast to elevations …

A Threshold Of Transmembrane Potential Is Required For Mitochondrial Dynamic Balance Mediated By Drp1 And Oma1, Edith Jones, Norma Gaytan, Iraselia Garcia, Alan Herrera, Manuel Ramos, Divya Agarwala, Maahrose Rana, Wendy Innis-Whitehouse, Erin Schuenzel, Robert Gilkerson

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

As an organellar network, mitochondria dynamically regulate their organization via opposing fusion and fission pathways to maintain bioenergetic homeostasis and contribute to key cellular pathways. This dynamic balance is directly linked to bioenergetic function: loss of transmembrane potential across the inner membrane (Dwm) disrupts mitochondrial fission/fusion balance, causing fragmentation of the network. However, the level of Dwm required for mitochondrial dynamic balance, as well as the relative contributions of fission and fusion pathways, have remained unclear. To explore this, mitochondrial morphology and Dwm were examined via confocal imaging and tetramethyl rhodamine ester (TMRE) flow cytometry, respectively, in cultured 143B osteosarcoma …

Mutational Analysis Of The Qrrq Motif In The Yeast Hig1 Type 2 Protein Rcf1 Reveals A Regulatory Role For The Cytochrome C Oxidase Complex, Joshua Garlich, Valentina Strecker, Ilka Wittig, Rosemary A. Stuart

Biological Sciences Faculty Research and Publications

The yeast Rcf1 protein is a member of the conserved family of proteins termed the hypoxia-induced gene (domain) 1 (Hig1 or HIGD1) family. Rcf1 interacts with components of the mitochondrial oxidative phosphorylation system, in particular the cytochrome bc₁(complex III)-cytochrome c oxidase (complex IV) supercomplex (termed III-IV) and the ADP/ATP carrier proteins. Rcf1 plays a role in the assembly and modulation of the activity of complex IV; however, the molecular basis for how Rcf1 influences the activity of complex IV is currently unknown. Hig1 type 2 isoforms, which include the Rcf1 protein, are characterized in part by the presence …

Where Did The Birds Go?, Brinnlie Harward, Kim Sullivan, Josh Wardle

Biology Posters

Birds on campus have had an extremely high death rate near University Inn in past years. The trees on the west side of the building are within the kill zone. The kill zone is 15-30 feet away from the building.

Honey Fungus: A Silent Killer, Michael Gudmundson, Jim Lutz

Biology Posters

Armillaria root disease is found in many temperate and tropical forests throughout the world. It is responsible for on average 2-3 % of mortalities in infected forests. This root disease is caused by many species of fungus from the Armillaria genus. They are commonly referred to as Honey Fungus. This fungus spreads mainly through the interaction of tree roots. The Wind River Forest Dynamics Plot shows evidence of an infection by Armillaria. However the Extent of the infection has not been studied or mapped. Research Question : How much of the WFDP is infected and what are the effects of …

Bird Strikes At Usu, Jonas Johnson, Kim Sullivan

Biology Posters

According to the American Bird Conservancy, up to one billion birds are killed in the United States every year by window collisions.

Global Analysis Of Population Growth And Decline, Mark Sneddon, William D. Pearse

Biology Posters

Species of plants and animals have populations that are declining at a rapid rate and possibly face extinction. To combat this decline, we must first understand where and why species are declining. We compared known species population growth rates in the COMPADRE (7024 different plant populations) and COMADRE (1927 different animal populations) databases to a variety of possible factors and other databases.

Transcript Levels Of Salt Tolerant Candidate Genes In Turfgrasses, Thomas Sprouse, Shaun Bushman, Paul Johnson

Biology Posters

Cool season turfgrasses have necessary functions in the landscape, but are associated with excessive water use. Cities have begun to reuse water for outdoor purposes as a response to a shrinking water supply, this ‘effluent’ water has higher salt content. This high salt content puts turfgrasses under increased stress. The focus of our research is to investigate the involvement of two genes in salt tolerance among turfgrass species: an H-type thioredoxin (Trx) that reduces oxidative damage within salt stressed cells, and a vacuolar (v-type) ATPase subunit that transports +H ions across the vacuole membrane to maintain osmotic potential under higher …

Will A Game Cause Increased Fruit/Vegetable Intake And Physical Activity In Elementary School Children?, Derek Trimble

Biology Posters

Background Incentives increase healthy lifestyle choices. Schools don’t have financial resources for an incentive program. Research Question Is there a way to increase fruit and vegetable intake and physical activity in elementary school children at little or no cost to the school?

Transformation Of Lactic Acid Bacteria, Bryan Coburn

Biology Posters

Lactococcus lactis (strain JB704) bacterial cells were transformed at 2500 Volts, 25 µF, and 200 Ω with the pTW8 vector. However, bacterial cells were grown and pre-treated differently. One culture of recipient cells was grown in MRS without 1% glycine and received no pre-treatment, were pre-treated for 30’ with water, or were pre-treated for 30’ with 100 mM lithium acetate and 10 mM dithiothreitol (DTT); a second culture of recipient cells was grown in MRS with 1% glycine and received no pre-treatment, were pretreated for 30’ with water, or were pre-treated for 30’ with 100 mM lithium acetate and 10mM …

Generation Of Polyclonal Rabbit Antisera Specific To The Zika Virus Capsid Protein, Michael C. Berentzen, Jordan C. Frank, Sang-Im Yun, Byung-Hak Song, Young-Min Lee

Biology Posters

Zika virus (ZIKV), a mosquito-borne flavivirus, is an emerging zoonotic pathogen closely related to Japanese encephalitis virus, West Nile virus, dengue virus, and yellow fever virus. Although ZIKV infection generally produces only mild symptoms in some infected individuals, it has recently been associated with a growing number of neurological diseases, including Guillain-Barré syndrome in ZIKV-infected adults and microcephaly in infants born to ZIKV-infected women. Like all flaviviruses, ZIKV has a plus-strand RNA genome encoding ten functional proteins (designated C, prM, E, NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5). Of these ten, the C (capsid) protein is an essential structural …

Virus Survival Without Hosts, Cody J. Bills, Craig W. Day

Biology Posters

It is important to always clean up using proper chemicals following working with a virus. Failure to do so may leave active virus on a surface or in a medium and present a potential hazard. This study was done to determine if viruses from the Flaviviridae, Adenoviridae, and Picornaviridae families can remain active for more than three weeks on a dry surface or in a liquid medium.

It Pays To Protect The Birds: Estimated Costs Of Collision-Proofing Skywalks On Usu Campus, Rachel Sagers, Kim Sullivan

Biology Posters

No abstract provided.

Synthesizing Spider Silk Protein From E. Coli, Mitchell Richwine

Biology Posters

Spider silk is a valuable material due to its extreme strength and elasticity. However, spider silk is difficult to produce in mass quantities because of the many factors which get in the way of producing large amounts of pure protein. Harvesting the silk from spiders is not efficient. A major issue is spiders like to kill each other until they have their own web space, so storing spiders is impractical, and the silk harvested from spiders does not allow for large quantities of the pure protein. Also the protein from spiders is only useful in producing fibers. The solution to …

Bird Strikes At The Chase Fine Arts Building, Michael Preece

Biology Posters

Up to one billion birds die each year by striking windows. Bird eyes do not interperet windows as solid objects. At Utah State, hundreds of birds die each year in bird strikes. One of the buildings that is responsible for a large portion of bird strikes is the Chase Fine Arts building. Fruit trees in front of the Chase Fine Arts windows cause a reflection which confuse birds A redution in window reflectivity greatly reduces bird strike problems.