Biology Commons^™

Sine-Wave Electrical Stimulation Initiates A Voltage-Gated Potassium Channel-Dependent Soft Tissue Response Characterized By Induction Of Hemocyte Recruitment And Collagen Deposition, Brandon M. Franklin, Eleni Maroudas, Jeffrey L. Osborn

Biology Faculty Publications

Soft tissue repair is a complex process that requires specific communication between multiple cell types to orchestrate effective restoration of physiological functions. Macrophages play a critical role in this wound healing process beginning at the onset of tissue injury. Understanding the signaling mechanisms involved in macrophage recruitment to the wound site is an essential step for developing more effective clinical therapies. Macrophages are known to respond to electrical fields, but the underlying cellular mechanisms mediating this response is unknown. This study demonstrated that low‐amplitude sine‐wave electrical stimulation (ES) initiates a soft tissue response in the absence of injury in Procambarus …

Ab Blood Type And Its Genetic Relationship To Alzheimer’S Disease, Mark Wadsworth, John S. K. Kauwe

Journal of Undergraduate Research

Alzheimer’s Disease (AD) is a debilitating disease that has increased the hardship of many lives. Many people above the age of 65 have been diagnosed with this genetically complex disease, which financially and emotionally burdens those diagnosed and their families. Earlier this year a study was released that found, using a dataset of individuals that have had strokes, that individuals with AB blood type are more at risk of developing cognitive impairment[1]. This study however was not looking at the genetics of AD. My objective in performing this study was to see if there is a genetic association between Alzheimer’s …

Song Types, Repertoires And Patterns Of Song Sharing Among Canyon Wrens, Catherpes Mexicanus, Anne Rose

Ursidae: The Undergraduate Research Journal at the University of Northern Colorado

No abstract provided.

Divergence Of Sulfur-Flower Buckwheat Using Dna Analyses, Taylor Brevik, Mitchell Mcglaughlin

Ursidae: The Undergraduate Research Journal at the University of Northern Colorado

No abstract provided.

Cloning Of Immunoglobulin Cdnas From The Jamaican Fruit Bat, Benjamin Lee

Ursidae: The Undergraduate Research Journal at the University of Northern Colorado

No abstract provided.

Staphylococcus Aureus Coordinates Leukocidin Expression And Pathogenesis By Sensing Metabolic Fluxes Via Rpirc, Divya Balasubramanian, Elizabeth A Ohneck, Jessica Chapman, Andy Weiss, Min Kyung Kim, Tamara Reyes-Robles, Judy Zhong, Lindsey N. Shaw, Desmond S. Lun, Beatrix Ueberheide, Bo Shopsin, Victor J Torres

Molecular Biosciences Faculty Publications

Staphylococcus aureus is a formidable human pathogen that uses secreted cytolytic factors to injure immune cells and promote infection of its host. Of these proteins, the bicomponent family of pore-forming leukocidins play critical roles in S. aureus pathogenesis. The regulatory mechanisms governing the expression of these toxins are incompletely defined. In this work, we performed a screen to identify transcriptional regulators involved in leukocidin expression in S. aureus strain USA300. We discovered that a metabolic sensor-regulator, RpiRc, is a potent and selective repressor of two leukocidins, LukED and LukSF-PV. Whole-genome transcriptomics, S. aureus exoprotein proteomics, and metabolomic analyses revealed that …

Alcohol Discrimination And Preferences In Two Species Of Nectar-Feeding Primate, Samuel R. Gochman, Michael B. Brown, Nathaniel J. Dominy

Dartmouth Scholarship

Recent reports suggest that dietary ethanol, or alcohol, is a supplemental source of calories for some primates. For example, slow lorises (Nycticebus coucang) consume fermented nectars with a mean alcohol concentration of 0.6% (range: 0.0–3.8%). A similar behaviour is hypothesized for aye-ayes (Daubentonia madagascariensis) based on a single point mutation (A294V) in the gene that encodes alcohol dehydrogenase class IV (ADH4), the first enzyme to catabolize alcohol during digestion. The mutation increases catalytic efficiency 40-fold and may confer a selective advantage to aye-ayes that consume the nectar of Ravenala madagascariensis. It is uncertain, however, whether alcohol exists in this nectar …

Loss Of Cell Adhesion Increases Tumorigenic Potential Of Polarity Deficient Scribble Mutant Cells, Indrayani Waghmare, Madhuri Kango-Singh

Biology Faculty Publications

Epithelial polarity genes are important for maintaining tissue architecture, and regulating growth. The Drosophila neoplastic tumor suppressor gene scribble (scrib) belongs to the basolateral polarity complex. Loss of scrib results in disruption of its growth regulatory functions, and downregulation or mislocalization of Scrib is correlated to tumor growth. Somatic scribble mutant cells (scrib-) surrounded by wild-type cells undergo apoptosis, which can be prevented by introduction of secondary mutations that provide a growth advantage. Using genetic tools in Drosophila, we analyzed the phenotypic effects of loss of scrib in different growth promoting backgrounds. We investigated if a central …

Freshwater Metaviromics And Bacteriophages: A Current Assessment Of The State Of The Art In Relation To Bioinformatic Challenges, Catherine Putonti, Katherine Bruder, Kema Malki, Alexandria Cooper, Emily Sible, Jason W. Shapiro, Siobhan C. Watkins

Biology: Faculty Publications and Other Works

Advances in bioinformatics and sequencing technologies have allowed for the analysis of complex microbial communities at an unprecedented rate. While much focus is often placed on the cellular members of these communities, viruses play a pivotal role, particularly bacteria-infecting viruses (bacteriophages); phages mediate global biogeochemical processes and drive microbial evolution through bacterial grazing and horizontal gene transfer. Despite their importance and ubiquity in nature, very little is known about the diversity and structure of viral communities. Though the need for culture-based methods for viral identification has been somewhat circumvented through metagenomic techniques, the analysis of metaviromic data is marred with …

The Other Lives Of Ribosomal Proteins, Metrah M. Nader

PSU McNair Scholars Online Journal

Cellular mRNAs are produced in the nucleus and exported to the cytoplasm through nuclear pore complexes (NPC) that are found in the nuclear membrane. The correctly processed mRNAs are exported in the form of messenger ribonucleoproteins (mRNPs). Since the NPC allows the passage of only one mRNA molecule at a time, export of large ribonucleoproteins (megaRNP) found in the nucleus cannot be transported through the NPC. However, a recent study has discovered an alternative export mechanism known as nuclear envelope budding, which allows megaRNP granules to be exported. This study particularly focuses on identifying ribosomal proteins (RPs) found within large …

Gene Expression Patterns Of Bone Morphogenetic Proteins (Bmps) During Early Embryonic Development In The Annual Killifish Austrofundulus Limnaeus, Eva Y. Chan

PSU McNair Scholars Online Journal

The developmental pattern of the annual killifish Austrofundulus limnaeus is unusual compared to other fish. First, the cell movements associated with gastrulation are separated from formation of the embryonic axis. In addition, embryonic diapause can occur. Diapause II and III are often observed when embryos are incubated at 25^oC. Maternal influences and incubation environment are the factors that determine entrance into embryonic diapause, but the molecular mechanisms that regulate diapause are still unknown. Interestingly, embryonic diapause and the unusual cell movements observed during early development are always found together in species of annual killifish that exhibit diapause. Bone …

Facilitating The Release Of Bionutrients From Morinda Citrifolia (Noni) By Cellulase Hydrolysis And Lactic Acid Bacteria Fermentation And Their Effects On Α-Amylase And Α-Glucosidase Activities, Hui-Hung Lee, Li-Jung Yin, Hsueh-Ming Tai, Shann-Tzong Jiang

Journal of Marine Science and Technology–Taiwan

For facilitating the release of bionutrients from noni, 6 h of hydrolysis with 100 U/mL cellulase at 50℃ and an additional 48 h of fermentation with Pediococcus pentosaceus BCRC 14053 at 37℃ were performed. Increases in the extraction yields (87.8-607.8 mg/g) and the total phenolic content (2.02-7.45 mg/g) of various extracts were obtained, suggesting that bionutrients were released from noni during hydrolysis or fermentation. In hydrolyzed and/or additionally fermented samples, significant decreases in the half maximal inhibitory concentration (IC50) against α-amylase (10.28-1.61 mg/mL) and α-glucosidase (46.47-3.73 mg/mL) were observed. Gas chromatography–mass spectrometry results revealed that the major components with these …

Histological And Molecular Analysis Of A Progressive Diffuse Intrinsic Pontine Glioma And Synchronous Metastatic Lesions: A Case Report., Javad Nazarian, Gary E Mason, Cheng Ying Ho, Eshini Panditharatna, Madhuri Kambhampati, Louis Gilbert Vezina, Roger J. Packer, Eugene I Hwang

Genomics and Precision Medicine Faculty Publications

There is no curative treatment for patients with diffuse intrinsic pontine glioma (DIPG). However, with the recent availability of biopsy and autopsy tissue, new data regarding the biologic behavior of this tumor have emerged, allowing greater molecular characterization and leading to investigations which may result in improved therapeutic options. Treatment strategies must address both primary disease sites as well as any metastatic deposits, which may be variably sensitive to a particular approach.In this case report, we present a patient with DIPG treated with irradiation and serial investigational agents. The clinical, pathological and molecular phenotypes of both the progressive primary tumor …

Nucleotide Interdependency In Transcription Factor Binding Sites In The Drosophila Genome, Jacqueline M. Dresch, Rowan G. Zellers, Daniel K. Bork, Robert A. Drewell

Biology

A long-standing objective in modern biology is to characterize the molecular components that drive the development of an organism. At the heart of eukaryotic development lies gene regulation. On the molecular level, much of the research in this field has focused on the binding of transcription factors (TFs) to regulatory regions in the genome known as cis-regulatory modules (CRMs). However, relatively little is known about the sequence-specific binding preferences of many TFs, especially with respect to the possible interdependencies between the nucleotides that make up binding sites. A particular limitation of many existing algorithms that aim to predict binding site …

Offspring Size-Number Tradeoffs And Food Quality Feedbacks Impact Population Dynamics In A Daphnia-Algae System, Ryan Wenkus

College of Science and Health Theses and Dissertations

Population fluctuations can be affected by a number of extrinsic and intrinsic factors, but few manipulative experiments have been conducted that can isolate these effects in consumer-resource systems. Extrinsic factors such as weather patterns or food availability can impact consumer growth and reproduction. Additionally, intrinsic factors relating to life history can have significant impacts on population growth rates. A fundamental principle of life history theory is that individuals are limited by trade-offs between survival and reproduction, and there are a variety of combinations, given environment and physiological limitations, that should maximize lifetime reproductive output. Reproductive strategies vary between species, but …

Home-Field Advantage? Evidence Of Local Adaptation Among Plants, Soil, And Arbuscular Mycorrhizal Fungi Through Meta-Analysis, Megan A. Rúa, Anita Antoninka, Pedro M. Antunes, V Bala Chaudhary, Catherine Gehring, Louis J. Lamit, Bridget J. Piculell, James D. Bever, Cathy Zabinski, James F. Meadow, Marc J. Lajeunesse, Brook G. Milligan, Justine Karst, Jason D. Hoeksema

Integrative Biology Faculty and Staff Publications

BACKGROUND: Local adaptation, the differential success of genotypes in their native versus foreign environment, arises from various evolutionary processes, but the importance of concurrent abiotic and biotic factors as drivers of local adaptation has only recently been investigated. Local adaptation to biotic interactions may be particularly important for plants, as they associate with microbial symbionts that can significantly affect their fitness and may enable rapid evolution. The arbuscular mycorrhizal (AM) symbiosis is ideal for investigations of local adaptation because it is globally widespread among most plant taxa and can significantly affect plant growth and fitness. Using meta-analysis on 1170 studies …

Mapping Morels: Predicting The Locations Of Morchella Species Through Environmental Factors Using The Gis System, Emily M. Stanevicius

Celebration of Learning

Morel mushrooms, Morechella esculenta and M. deliciosa, are known delicacies across the globe, ranging from exquisite dishes in French cuisine to Eastern palates such as Japanese Matsutake. According to literature, true morels diverged as their own genus about 129 million years, again which has led to the development of more than 177 species and have been part of the human diet since their beginning. However, the elusiveness of morels has contributed to the mushrooms infamy for rarity, and has even been known to sell for more than $40 per pound. This project seeks to aid in the search for morels …

Semaphorin3a Increases Focal Adhesion Formation To Shift The Relationship Between Cell Migration And Substratum Concentration Through A Rock-Dependent Mechanism, Frances V. Compere, Scott Gehler

Celebration of Learning

Cell migration is essential for many life processes, including wound healing, embryonic development and cancer metastasis. Cells move across a surface by interacting and forming adhesions with the molecules in their environment, specifically the extracellular matrix. Past studies have shown that there is an optimal level of cell-substratum adhesive strength that allows for the most cell migration and spreading (DiMilla et al., 1993; Gaudet et al., 2003). The mechanism by which this works is not well understood, however. Semaphorin 3A (Sema3A) has been shown to increase the expression of integrin receptors, which help mediate the formation of the adhesions between …

Investigating The Origin And Functions Of A Novel Small Rna In Escherichia Coli, Fenil Rashmin Kacharia

Dissertations and Theses

Non-coding small RNAs (sRNAs) regulate various cellular processes in bacteria. They bind to a chaperone protein Hfq for stability and regulate gene expression by base-pairing with target mRNAs. Although the importance of sRNAs in bacteria has been well established, the mode of origination of novel sRNA genes is still elusive, mainly because the rapid rate of evolution of sRNAs obscures their original sources. To overcome this impediment, we identified a recently formed sRNA (EcsR2) in E. coli, and show that it evolved from a degraded bacteriophage gene. Our analyses also revealed that young sRNAs such as EcsR2 are expressed …

Human Locomotion: Examining Energy Costs And Human Behavior Associated With Load-Carrying, Gailynn Benjamin

Honors Projects

The energy expenditure during load-carrying depends on the mass of the load, the position of the load on the body, and if tools are used to help transfer the load. When the load being carried is a human child, strategies employed to cope with the increased energetic burden should include males carrying older and heavier children, arm use decreasing as children become older, and tool use increasing as children become older. To uncover whether people make energetically-mediated decisions to carry children, 236 adult males and 314 adult females were observed walking around parks in Seattle, WA, while carrying a child. …

Cmg Helicase Assembly And Activation: Regulation By C-Myc Through Chromatin Decondensation And Novel Therapeutic Avenues For Cancer Treatment, Victoria Bryant

USF Tampa Graduate Theses and Dissertations

The CMG (Cdc45, MCM, GINS) helicase is required for cellular proliferation and functions to unwind double-stranded DNA to allow the replication machinery to duplicate the genome. Cancer cells mismanage helicase activation through a variety of mechanisms, leading to the potential for the development of novel anti-cancer treatments. Mammalian cells load an excess of MCM complexes that act as reserves for new replication origins to be created when replication forks stall due to stress conditions, such as drug treatment. Targeting the helicase through inhibition of the MCM complex has sensitized cancer cells to drugs that inhibit DNA replication, such as aphidicolin …

Effect Of Different Miracle-Gro Concentrations On The Growth Of Wisconsin Fast Plants, Eric D. Shershen, Nicholas M. Orslini, David Channon, Marvin Agyeben

Celebration of Learning

The Wisconsin Fast Plant, Brassica rapa (Brassicaceae) seeds were planted in three, six-celled containers and were filled with standard potting soil under continuous grow-lux lighting. After the first week of plant growth, E1and E2 were placed in a separate trays and given 1.0 and 1.5x the recommended amount of Miracle-Gro formula, respectively. The control was left in tap water and all were kept in 1 cm of solution. Every seven days the plants’ heights were recorded, and the total number of flowers were counted. By the fourth week, the average height of the control plants was 105.7 mm, of the …

Effects Of Mutating The Mrub_1345 Gene Found In Meiothermus Ruber, Brandon M. Wills, Lori R. Scott

Celebration of Learning

Meiothermus ruber is a unique, red-pigmented, thermophilic bacterium that preferentially grows in high-temperature environments ranging from 35-70°C. Due to the lack of studies performed on this organism, there is quite a bit of information missing in regard to the genes found within this organism’s genome and their function. This study focuses in on the Mrub_1345 gene in M. ruber, which has been suggested to be orthologous to the proC gene found of E. coli proline biosynthesis pathway. To test if these genes are orthologs, we performed the complementation assay on wild-type proC. Next, we performed site-directed mutagenesis on amino acids …

Sources And Transformations Of Dissolved Lignin Phenols And Chromophoric Dissolved Organic Matter In Otsuchi Bay, Japan, Chia-Jung Lu, Ronald Benner, Cedric Fichot, Hideki Fukuda, Youhei Yamashita, Hiroshi Ogawa

Faculty Publications

Dissolved lignin phenols and optical properties of dissolved organic matter (DOM) were measured to investigate the sources and transformations of terrigenous DOM (tDOM) in Otsuchi Bay, Japan. Three rivers discharge into the bay, and relatively high values of syringyl:vanillyl phenols (0.73 ± 0.07) and cinnamyl:vanillyl phenols (0.33 ± 0.10) indicated large contributions of non-woody angiosperm tissues to lignin and tDOM. The physical mixing of river and seawater played an important role in controlling the concentrations and distributions of lignin phenols and chromophoric DOM (CDOM) optical properties in the bay. Lignin phenol concentrations and the CDOM absorption coefficient at 350 nm, …

Shell Bluff – A Fossiliferous Ridge, The Site Of The Extinct Oyster Crassostrea Gigantissima And History Of Its Identification, Elliott O. Edwards Jr.

Georgia Journal of Science

Shell Bluff is described by geologists as stratigraphically one of the most important exposures in the Georgia Coastal Plain because the bluff is home to the large oyster, Crassostrea gigantissima, now extinct. Native Americans inhabited this area prior to Hernando DeSoto and his men who visited the area in 1540. They were probably the first Europeans to visit Shell Bluff. John and son William Bartram visited the bluff in 1764 and John described the bluff in his journal and the existence of large oysters. The British naturalist, John Finch, described a fossil oyster taken from the site in 1824 as …

Population Viability Analysis For Endangered Roanoke Logperch, James Henry Roberts, Paul L. Angermeier, Gregory B. Anderson

Biology: Faculty Publications

A common strategy for recovering endangered species is ensuring that populations exceed the minimum viable population size (MVP), a demographic benchmark that theoretically ensures low long-term extinction risk. One method of establishing MVP is population viability analysis, a modeling technique that simulates population trajectories and forecasts extinction risk based on a series of biological, environmental, and management assumptions. Such models also help identify key uncertainties that have a large influence on extinction risk. We used stochastic count-based simulation models to explore extinction risk, MVP, and the possible benefits of alternative management strategies in populations of Roanoke logperch Percina rex, an …

Trypanosoma Brucei Tif2 And Trf Suppress Vsg Switching Using Overlapping And Independent Mechanisms, Sanaa E. Jehi, Vishal Nanavaty, Bibo Li Ph.D.

Biological, Geological, and Environmental Faculty Publications

Trypanosoma brucei causes debilitating human African trypanosomiasis and evades the host's immune response by regularly switching its major surface antigen, VSG, which is expressed exclusively from subtelomeric loci. We previously showed that two interacting telomere proteins, TbTRF and TbTIF2, are essential for cell proliferation and suppress VSG switching by inhibiting DNA recombination events involving the whole active VSG expression site. We now find that TbTIF2 stabilizes TbTRF protein levels by inhibiting their degradation by the 26S proteasome, indicating that decreased TbTRF protein levels in TbTIF2-depleted cells contribute to more frequent VSG switching and eventual cell growth arrest. Surprisingly, although TbTIF2 …

A Long-Term Study Of Ecological Impacts Of River Channelization On The Population Of An Endangered Fish: Lessons Learned For Assessment And Restoration, James Henry Roberts, Gregory B. Anderson, Paul L. Angermeier

Biology: Faculty Publications

Projects to assess environmental impact or restoration success in rivers focus on project-specific questions but can also provide valuable insights for future projects. Both restoration actions and impact assessments can become “adaptive” by using the knowledge gained from long-term monitoring and analysis to revise the actions, monitoring, conceptual model, or interpretation of findings so that subsequent actions or assessments are better informed. Assessments of impact or restoration success are especially challenging when the indicators of interest are imperiled species and/or the impacts being addressed are complex. From 1997 to 2015, we worked closely with two federal agencies to monitor habitat …

Mechanisms Of Immune Activation And Suppression By Parasitic Wasps Of Drosophila, Johnny R. Ramroop

Dissertations, Theses, and Capstone Projects

Drosophila melanogaster has served as an excellent model organism to study the molecular processes of innate immunity. Flies essentially lack adaptive immunity and the innate immune system is often divided into the humoral and cellular responses (Lemaitre and Hoffmann 2007). The humoral arm involves the production of antimicrobial peptides, secreted from the fat body, to combat bacterial and fungal infections. The cellular response involves the production of hemocytes (blood cells: crystal cells, plasmatocytes, and lamellocytes) in the larval lymph gland, in the sessile pools, and in circulation (Gold and Bruckner 2014). Microbial pathogens are phagocytosed by plasmatocytes whereas larger parasites …

Effect Of Cntf Derived Peptide, P021 On Cognition And Pathology In 3xtg-Ad Mouse Model Of Alzheimer's Disease, Narjes Baazaoui

Dissertations, Theses, and Capstone Projects

Studies described in this thesis deal with the preventive effects of a neurogenic/neurotropic peptidergic compound, P021, on neurogenesis and synaptic deficits, neurodegeneration, cognitive impairment, and Ab and tau pathologies in a 3xTg-AD mouse model of Alzheimer’s disease (AD).

Background: AD is a chronic progressive neurodegenerative disease. Its multifactorial nature and the heterogeneity make its treatment especially challenging. Although it is a major burden in society, at present there is no drug that can stop or slow down the progression of the disease. Currently, the only available treatments are symptomatic and for mild to severe stages. The development of a drug …