Biology Commons^™

Macgillivray's Seaside Sparrow (Ammodramus Maritimus Macgillivraii) Breeding Biology & Population Density At Tom Yawkey Wildlife Center, Yianni P. Laskaris

Electronic Theses and Dissertations

MacGillivray's Seaside Sparrow (Ammodramus maritimus macgillivraii) is a coastal marsh species sensitive to habitat alteration and threatened by coastal wetland loss. Data on seaside sparrow distribution, breeding success, and critical habitats are lacking in South Carolina. To better understand the demographics and incubation behavior of the species, I monitored breeding success, densities, song output, and presence of predators at three 7-hectare plots at the densest known population in the state. I also placed temperature data-loggers in a subset of nests to discern any links between incubation behavior and nest fate. Densities varied from a low of 5 birds/ha in a …

Flying Under The Lidar: Relating Forest Structure To Bat Community Diversity, Anna Schneider Swanson

Electronic Theses and Dissertations

Bats are important to many ecological processes such as pollination, insect (and by proxy, disease) control, and seed dispersal and can be used to monitor ecosystem health. However, they are facing unprecedented extinction risks from habitat degradation as well as pressures from pathogens (e.g., white-nose syndrome) and wind turbines. LiDAR allows ecologists to measure structural variables of forested landscapes with increased precision and accuracy at broader spatial scales than previously possible. This study used airborne LiDAR to classify forest habitat/canopy structure at the Ordway-Swisher Biological Station (OSBS) in north central Florida. LiDAR data were acquired by the National Ecological Observatory …

Accessible Bioprinting: Adaptation Of A Low-Cost 3d-Printer For Precise Cell Placement And Stem Cell Differentiation, John A. Reid, Peter A. Mollica, Garett D. Johnson, Roy C. Ogle, Robert D. Bruno, Patrick C. Sachs

School of Medical Diagnostics & Translational Sciences Publications

The precision and repeatability offered by computer-aided design and computer-numerically controlled techniques in biofabrication processes is quickly becoming an industry standard. However, many hurdles still exist before these techniques can be used in research laboratories for cellular and molecular biology applications. Extrusion-based bioprinting systems have been characterized by high development costs, injector clogging, difficulty achieving small cell number deposits, decreased cell viability, and altered cell function post-printing. To circumvent the high-price barrier to entry of conventional bioprinters, we designed and 3D printed components for the adaptation of an inexpensive 'off-the-shelf' commercially available 3D printer. We also demonstrate via goal based …

Investigation Of The Expression Of The Haesa Receptor-Like Kinase As Regulated By The Stm And Ath1 Homeodomain Transcription Factors In Arabidopsis Thaliana, Darby Raybourn

Honors Theses

Abscission, the shedding of plant organs, is a key process in plant development. Abscission takes place in highly regulated regions called abscission zones (AZ). ARABIDOPSIS THALIANA HOMEOBOX GENE1 (ATH1) and SHOOT MERISTEMLESS (STM) are organ boundary transcription factors that have previously been observed to play a role in sepal-stem boundary formation. Plants carrying mutations in both genes have flowers in which the sepal-stem boundary is abolished. The double mutant flowers are also observed to retain all floral organs. Because abscission zones in Arabidopsis form at organ boundary regions, we hypothesized that the STM and ATH1 transcription factors play a role …

Intrinsic And Extrinsic Factors Mediating The Expression Of Alternative Mating Tactics In Male Green Treefrogs, Hyla Cinerea, Aleah R. Liddell

Honors Theses

Males of many anuran species optimize their chances of reproductive success by vigorously calling to attract females or by adopting a non-calling satellite mating tactic. Satellite males perch silently near calling males and attempt to intercept females that the caller attracts. Models predict that tactic expression in male anurans is influenced by either extrinsic factors (i.e., the social-acoustic environment) or intrinsic factors (i.e., circulating hormone levels and body condition), but it is unclear how these factors potentially interact to influence tactic expression. For example, males often adopt a satellite mating tactic in response to the broadcast vocalizations of conspecific males, …

The Effects Of Tillage, Glyphosate, And Genetic Modification On Bacterial Root Endophyte Composition In Zea Mays, Breena Lori Nolan

Honors Theses

The community structure of the endophytic bacteria in Zea mays roots was examined for the potential effects of glyphosate application, tillage strategies, and whether or not the corn plant in question was of an organic or glyphosate-resistant variety. Roots were harvested from plots designated to receive their specific treatments at the USDA-ARS Crop Production Systems Research Unit Farm. Vortexing, sonication, and tissue grinding, extraction, and next generation sequencing of 16S rRNA genes from these roots were used to describe their bacterial community composition. Results indicated significant differences in the bacterial communities correlated to tillage practice or corn type, whereas glyphosate …

Methods Of Quantification And Characterization Of Coccidian Oocysts, Mariam Abunemeh

Honors Theses

Coccidiosis is a major economic and health risk in the poultry industry. The oocysts of the causative agent of coccidiosis are excreted in animal feces and must be ingested by a new host for a new infection to begin. These oocysts are microscopic and very similar between species. The ability to quantify and identify the oocysts that are causing the illness is important to controlling this disease. My research first compares methods of quantifying oocysts of domestic turkeys for their ease of use and accuracy. Next, I attempt to identifying novel oocysts from a different turkey species by morphological and …

Christian Faith And Evolution: One Person's Journey In Science And In Faith, Logan Wilson

Honors Theses

Evolution has always proved to be controversial to many people of faith, but it has been specifically problematic to many Christians including myself. This thesis explores the history and substance of the controversy between different expressions of Christianity and evolution by providing a relatable rendition of the theory of evolution. It breaks down the contributions of Charles Darwin and explains them in a manner than can be understood by a non-biologist. It also explores the stances of certain authors such as Richard Dawkins and Michael Ruse on the matter. This thesis is an independent study comprised of input from articles, …

Courtship Lateralization And Its Effect On Mating Success Of Male Wild Turkeys (Meleagris Gallopavo), Mara E. Vernier

Honors Theses

Lateralization results from unequal processing of tasks in the different hemispheres of the brain. While lateralization is a widely researched topic of study, little is known about the effects of sexual selection on lateralization. The purpose of this study was to determine if there is lateralization of male wild turkey (Meleagris gallopavo) courtship and whether the presence of lateralization is associated with male mating success. Male behavioral data were collected from video recordings of courtship made by Dr. Richard Buchholz during a previous mating study. Males were categorized as either successful or unsuccessful males based on how often they were …

Wastewater Treatment Plants: A Comparison Of Three Different Plants And Their Processes, Facilities, And Challenges They Face, Jon Abigail Mcdonald

Honors Theses

This study compared methods of wastewater treatment, and the conditions that they must be able to accommodate. To address this issue I visited wastewater treatment plants in three locations: Clarksdale, MS; University of Mississippi; and Oxford, MS. Additionally, I examined the chemistry and microbiology of the water in their respective receiving streams. I hypothesized that the wastewater plant with the most modern facilities and resources available would have the least effect on the water quality of its receiving stream. At each plant I learned what chemicals, equipment, and other methods are used, as well as unique challenges their treatment systems …

Analysis Of Joint Dysfunction During An Aging- Simulation, Laboratory Exercise For Allied Health Students, Anna E. O'Connor

Honors Theses

The aim of this experiment was to (1) develop and assess a joint-dysfunction simulation learning and sensitization activity for a Human Anatomy and Physiology I lab on joint structure and function (Part A) and (2) quantify the range of motion experienced during the simulation (Part B). The lab protocol allowed 318 allied health students to experience some of the physical limitations of aging, specifically arthritis, by asking them to perform activities of daily living (ADLs) while wearing simulation equipment that inhibited their range of movement. In Part A, students completed a pre- simulation survey, which assessed the students' knowledge about …

Elevated Corticosterone Levels Decrease Vocal Attractiveness And Elicit Non-Calling Mating Tactics In Male Green Tree Frogs, Hyla Cinerea, Hannah Burroff

Honors Theses

Condition-dependent male alternative tactics are often driven by competition among males for females. The expression of alternative male mating tactics can be influenced by the external social environment as well as internal physiological state. Recent models predict that circulating levels of stress hormones (i.e., glucocorticoids) and androgens may be particularly important in determining mating tactic expression under varying social circumstances. This study intergrates both components to investigate the cause of alternative tactic adoption in male green tree frogs, Hyla cinerea. Males of this species produce acoustic signals that are critical for attracting females and male vocalizations stimulate production of the …

Role-Playing In A Vaccination Debate Strengthens Student Scientific Debate Skills For Various Audiences, Gianne Souza, P. F. Mixter

Biology Faculty Publications

Students are surrounded by strongly-held viewpoints on scientific topics and frequently discuss news reports with their classmates. We developed the vaccination debate exercise to leverage this interest and develop core higher-order cognitive skills (HOCS), including, but not limited to, the ability to critique public media or primary research sources and create arguments for defending multiple viewpoints. Students prepared to debate different sides of the topic and then randomly assumed one of the roles: “Physician” (pro-vaccine), “Activist” (anti-vaccination), or “Parent-on-the-fence” (undecided). Students reported an increase in their abilities to discuss scientific topics with diverse audiences and an increased awareness of importance …

Ten Simple Rules For Digital Data Storage, E. M. Hart, P. Barmby, D. Lebauer, F. Michonneau, S. Mount, P. Mulrooney, T. Poisot, K. H. Woo, Naupaka B. Zimmerman, J. W. Hollister

Biology Faculty Publications

No abstract provided.

Altitudinal Shifts Of The Native And Introduced Flora Of California In The Context Of 20th-Century Warming, A. Wolf, Naupaka B. Zimmerman, W. R. Anderegg, P. E. Busby, J. Christensen

Biology Faculty Publications

Aim: The differential responses of plant species to climate change are of great interest and grave concern for scientists and conservationists. One underexploited resource for better understanding these changes are the records held by herbaria. Using these records to assess the responses of different groups of species across the entire flora of California, we sought to quantify the magnitude of species elevational shifts, to measure differences in shifts among functional groups and between native and introduced species, and to evaluate whether these shifts were related to the conservation of thermal niches.

Location: California.

Methods: To characterize these shifts in California, …

Neural And Neuroendocrine Processing Of A Non-Photic Cue In An Opportunistically Breeding Songbird, Darcy K. Ernst, G. E. Bentley

Biology Faculty Publications

Recent studies of the onset of breeding in long-day photoperiodic breeders have focused on the roles of type 2 and 3 iodothyronine deiodinases (DIO2 and DIO3) in the conversion of thyroxine (T4) to triiodothyronine (T3) and subsequent activation of the reproductive axis. It has been hypothesized that an increase in DIO2 and a reciprocal decrease in DIO3 causes the release of gonadotropinreleasing hormone (GnRH) from the hypothalamus, setting off a reproductive cascade, and that this DIO mechanism for GnRH release is conserved across vertebrate taxa. We sought to test whether social cues that are known to stimulate reproductive behaviors can …

The High Cost Of Reproduction In Sea Otters Necessitates Unique Physiological Adaptations, Nicole Thometz, T. L. Kendall, B. P. Richter, T. M. Williams

Biology Faculty Publications

Resting Superimposed on inherently high basal metabolic demands, the additional energetic requirements of reproduction can push female sea otters beyond physiological limits. Indeed, the resulting energy imbalance contributes to disproportionately high rates of mortality at the end of lactation in this species. To examine and quantify metabolic changes associated with reproduction, we measured the resting metabolic rate (RMR) of a female sea otter across gestation, lactation and non-reproductive periods. Concurrently, measurements were made on a non-breeding control female. Our results suggest that RMR declines during gestation. Conversely, RMR increases during lactation, reaches a peak at 3–4 months postpartum, and remains …

Comparative And Cumulative Energetic Costs Of Odontocete Responses To Anthropogenic Disturbance, Dawn P. Noren, Marla M. Holt, Robin C. Dunkin, Nicole Thometz, Terrie M. Williams

Biology Faculty Publications

Odontocetes respond to vessels and anthropogenic noise by modifying vocal behavior, surface active behaviors, dive patterns, swim speed, direction of travel, and activity budgets. Exposure scenarios and behavioral responses vary across odontocetes. A literature review was conducted to determine relevant sources of disturbance and associated behavioral responses for several odontocete species (bottlenose dolphin, killer whale, harbor porpoise, and beaked whales). The energetic costs of species-specific responses to anthropogenic disturbance were then estimated. The energetic impact varies across species and scenarios as well as by behavioral responses. Overall, the cumulative energetic cost of ephemeral behavioral responses (e.g., performing surface active behaviors, …

Determining The Role Of Poly [Adp-Ribose] Polymerase 1 (Parp-1) In Retroviral Infection, Luis Valdes Rodriguez

Open Access Theses & Dissertations

Possible links between PARP-1 and HIV-1 have been previously reported but the role of this protein during viral infection has remained elusive. Based on the high evolutionary conservation of PARP-1, from Drosophila to humans, we have been able to counteract the difficulties related with the functional redundancy manifested in the PARP family by studying the influence of PARP-1 on the expression of an endogenous retrovirus using the chicken B lymphoblastoid cell line DT40, this cell line exhibits low PARP-1 functional redundancy and is viable after PARP-1 knockout. We have determined that the transcription level of the endogenous retrovirus RAV-1 is …

The Role Of The Dopamine/Ecdysteroid Receptor Dopecr In Ethanol Induced Behavioral Disinhibition, Gissel Pryscilla Aranda

Open Access Theses & Dissertations

Ethanol, a main active ingredient of alcoholic beverages, exerts numerous effects on behavior through its interaction with diverse membrane and signaling molecules and effector cells. The effects include lack of motor control, behavioral disinhibition, tolerance, sensitization and addiction. In particular, behavioral disinhibition is typically associated with heavy drinking and can lead to detrimental consequences such as car accidents, violent rages, risky sexual behavior and illegal substance abuse. This research aimed to clarify the neural elements and cellular mechanisms underlying behavioral disinhibition induced by ethanol. The neurotransmitter dopamine (DA) is implicated in ethanol-induced behavioral disinhibition (Van Gaalen et al., 2006). To …

Comparative Proteomic Analysis Of Extracellular Vesicles From Prostate Cancer-Derived Cell Lines, Gloria Polanco

Open Access Theses & Dissertations

Prostate cancer (PCa) is the leading non-cutaneous malignancy and the second deadliest among American men. PCa mortality rates among African American men are much higher than any other ethnic group, and the same is true for men of African ancestry world-wide. There is also a lack of reliable diagnostic markers and effective treatment options. Extracellular vesicles (EVs) have been observed to play an important role in cancer processes such as promotion of tumor growth and metastasis. They are also a promising source of diagnostic markers. This study addresses these problems by studying the proteome of EVs derived from PCa cells …

Phylogeography Of The Natal Puddle Frog Phrynobatrachus Natalensis (Anura: Phrynobatrachidae) In Sub-Saharan Africa, Joshua Adan Lara

Open Access Theses & Dissertations

The Family Phrynobatrachidae (Laurent, 1941) has one genus (Phrynobatrachus, Günther, 1862) containing 89 species. Commonly known as puddle frogs, they comprise one of the most species rich sub-Saharan amphibian groups. The objective of this study was to investigate P. natalensis from the Albertine Rift (AR) with systematic and phylogeographic methods. One mitochondrial DNA gene (16S = 555 base pairs [bp]) and one nuclear gene (RAG1 = 774 bp) were analyzed for 61 representatives of P. natalensis. Nine cryptic lineages were identified from Angola, Burundi, Côte d’Ivoire, Democratic Republic of the Congo, Ethiopia, Ghana, Mozambique, Namibia, Tanzania, Rwanda, and Uganda. Several …

Elucidating The Molecular Mechanism Of Action By Which Rs1-208b Induces Apoptosis In Hematological Cancers, Ruben Israel Calderon

Open Access Theses & Dissertations

The 26S proteasome has been successfully targeted to treat multiple myeloma. However, the effect can sometimes be reversed as cells acquire resistance to chemotherapy. Recently, combining proteasome inhibitors with indirect proteasome inhibitors has been proposed in order to delay and even reverse resistance. Small molecules derived from 4-piperidinone that maintain an unsaturated β-carbonyl group are thought to interact with the thiol group of catalytically active cysteine residues in deubiquitinases that associate with the 26S proteasome, effectively inhibiting the proteasome.

RS1-208b was selected following a high-throughput screening of a small library of 4-piperidinone derived curcumin structural analogs due to its selectivity …

Gβγ Dependent Modulation Of Cytoskeleton And Neurite Outgrowth Involves The Pi3k/Akt/Gsk-3Β Pathway, Jose L. Varela

Open Access Theses & Dissertations

Assembly and disassembly of microtubules is critical for axon and dendrite formation and neurite outgrowth. Previous studies have shown that βγ subunits of heterotrimeric G proteins promote microtubule (MT) assembly in vitro and in cultured PC12 cells. Recently, it was found that the interaction of Gβγ with MTs is important for nerve growth factor (NGF)-induced neuronal differentiation of PC12 cells and that the blocking of the interaction between Gβγ and tubulin/MT disrupted MTs, inhibited neurite outgrowth and induced axonal damage indicating the involvement of Gβγ in these processes. A different pathway involving the receptor tyrosine kinase (TrkA) and its downstream …

Comparing Process-Based And Constraint-Based Approaches For Modeling Macroecological Patterns, Xiao Xiao

Biology Faculty Publications

Ecological patterns arise from the interplay of many different processes, and yet the emergence of consistent phenomena across a diverse range of ecological systems suggests that many patterns may in part be determined by statistical or numerical constraints. Differentiating the extent to which patterns in a given system are determined statistically, and where it requires explicit ecological processes, has been difficult. We tackled this challenge by directly comparing models from a constraint-based theory, the Maximum Entropy Theory of Ecology (METE) and models from a process-based theory, the size-structured neutral theory (SSNT). Models from both theories were capable of characterizing the …

Diagnostic Doses And Times For Phlebotomus Papatasi And Lutzomiya Longipalpis Sand Flies (Diptera: Psychodidae: Phleboominae) Using The Cdc Bottle Bioassay To Assess Insecticide Resistance, David S. Denlinger, Joseph A. Creswell, J. Laine Anderson, Connor K. Reese, Scott A. Bernhardt

Biology Faculty Publications

Background: Insecticide resistance to synthetic chemical insecticides is a worldwide concern in phlebotomine sand flies (Diptera: Psychodidae), the vectors of Leishmania spp. parasites. The CDC bottle bioassay assesses resistance by testing populations against verified diagnostic doses and diagnostic times for an insecticide, but the assay has been used limitedly with sand flies. The objective of this study was to determine diagnostic doses and diagnostic times for laboratory Lutzomyia longipalpis (Lutz & Nieva) and Phlebotomus papatasi (Scopoli) to ten insecticides, including pyrethroids, organophosphates, carbamates, and DDT, that are used worldwide to control vectors.

Methods: Bioassays were conducted in 1,000-ml …

Managing The Spread Of Alfalfa Stem Nematodes (Ditylenchus Dipsaci): The Relationship Between Crop Rotation Periods And Pest Re-Emergence, S. Jordan, Claudia Nischwitz, Ricardo A. Ramirez, L. Gordillo

Biology Faculty Publications

Alfalfa is a critical cash/rotation crop in the western region of the United States, where it is common to find crops affected by the alfalfa stem nematode (Ditylenchus dipsaci). Understanding the spread dynamics associated with this pest would allow end-users to design better management programs and farming practices. This is of particular importance given that there are no nematicides available against alfalfa stem nematode and control strategies largely rely on crop rotation to non-host crops or by planting resistant varieties. I present a basic host-parasite model that describes the spread of the alfalfa stem nematode on alfalfa crops. With this …

Fluorescent Nanocomposite Of Embedded Ceria Nanoparticles In Crosslinked Pva Electrospun Nanofibers, Nader Shehata, Soha Gaballah, Effat Samir, Aya Hamed, Marwa Saad

Biology Faculty Publications

This paper introduces a new fluorescent nanocomposite of electrospun biodegradable nanofibers embedded with optical nanoparticles. In detail, this work introduces the fluorescence properties of PVA nanofibers generated by the electrospinning technique with embedded cerium oxide (ceria) nanoparticles. Under near-ultra violet excitation, the synthesized nanocomposite generates a visible fluorescent emission at 520 nm, varying its intensity peak according to the concentration of in situ embedded ceria nanoparticles. This is due to the fact that the embedded ceria nanoparticles have optical tri-valiant cerium ions, associated with formed oxygen vacancies, with a direct allowed bandgap around 3.5 eV. In addition, the impact of …

Association Of The Female Of Perdita (Xeromacrotera) Cephalotes (Cresson), And A Replacement Name For Perdita Bohartorum Parker (Hymenoptera: Andrenidae), Zach Portman, Terry L. Griswold, James P. Pitts

Biology Faculty Publications

The monotypic Perdita subgenus Xeromacrotera Timberlake is currently known only from the male sex. Here, the female of Perdita (Xeromacrotera) cephalotes (Cresson, 1878) is associated, resulting in two new junior synonyms of P. (X.) cephalotes: Perdita (Procockerellia) excellens Timberlake, 1958, new synonym and Perdita (Cockerellia) autumnalis Timberlake, 1977, new synonym. The true number of maxillary palpi are reported, and updated subgeneric and species diagnoses are provided. In addition, Perdita boharti Portman & Griswold, new name, is designated as a replacement name for the primary junior homonym P. bohartorum Parker, 1983 …

Assessing The Success Of Outreach At Preventing The Movement Of Aquatic Invasive Species In Illinois, Ellen Anne Cole

Master's Theses

Overland transport of recreational boats is the major vector of dispersal for aquatic invasive species among North American waterbodies. These species have large economic and ecosystem impacts, and understanding their movement is essential to understanding the biodiversity and functioning of aquatic ecosystems across the continent. I surveyed Illinois boaters to determine patterns of recreational boat movement and the consistency with which boaters perform actions to prevent the spread of invasive species. Although most boaters perform these actions, a large minority do not and the network for invasive species spread on recreational boats still connects most waterbodies. Next, I interviewed managers …