Biology Commons^™

Rare Genetic Variant To The Protection Of Alzheimer’S Disease, Josue David Gonzalez Murcia, John Kauwe

Journal of Undergraduate Research

Alzheimer’s disease is one of the top 10 causes of death in the world and the 6th leading cause of death in the United States, with 5.5 million people diagnosed with the disease. Alzheimer’s cannot be cured, prevented or slowed. Most research with Alzheimer’s disease has been done on SNPs that have a relatively high frequency in the population this has helped facilitate researchers in finding a broad genetic pathway in the development of Alzheimer’s disease (Naj et al. 2011; Harold et al. 2009). Our research will focus on a very rare SNP, with a much lower frequency than previously …

Molecular Diversity And Gene Evolution Of The Venom Arsenal Of Terebridae Predatory Marine Snails, Juliette Gorson, Girish Ramrattan, Aida Verdes, Elizabeth M. Wright, Yuri Kantor, Ramakrishnan Rajaram Srinivasan, Raj Musunuri, Daniel Packer, Gabriel Albano, Wei-Gang Qiu, Mandë Holford

Publications and Research

Venom peptides from predatory organisms are a resource for investigating evolutionary processes such as adaptive radiation or diversification, and exemplify promising targets for biomedical drug development. Terebridae are an understudied lineage of conoidean snails,which also includes cone snails and turrids. Characterization of cone snail venompeptides, conotoxins, has revealed a cocktail of bioactive compounds used to investigate physiological cellular function, predator-prey interactions, and to develop novel therapeutics. However, venom diversity of other conoidean snails remains poorly understood. The present research applies a venomics approach to characterize novel terebrid venom peptides, teretoxins, from the venom gland transcriptomes of Triplostephanus anilis and Terebra …

Triatominae (Hemiptera:Reduviidae) In Texas: Mitochondrial Genome Assembly, Trypanosoma Cruzi Detection, And Bacterial Community Analysis, Chissa-Louise Rivaldi

Biology Theses

The genus Triatoma contains vectors of the protozoan parasite Trypanosoma cruzi, the pathogen responsible for Chagas disease. The following research presents several inductive studies to develop vector control approaches targeted to the genus or species level. Two-hundred and eighty-two insects were collected, identified, and tested for the presence of T. cruzi. Four species of the Triatoma genus were collected - the overall incidence of T. cruzi was 52% (T. gerstaeckeri -51%, T. lecticularia – 92%, other, 29%). From these 282 insects, the bacterial communities of eight specimens of T. gerstaeckeri were sequenced and analyzed using pyrosequencing technology. The bacterial communities …

Transferring Hypothetical Deductive Reasoning, Jordan Hatch, Jamie L. Jensen

Journal of Undergraduate Research

The purpose of our project was to understand if hypothetical deductive (HD) reasoning skills exist in each discipline and if so, whether they are transferable between disciplines. HD reasoning is the method of scientific investigation used most commonly by scientists. It includes formulating hypotheses, devising experiments, making predictions, and analyzing results (iSTAR 2013). Many science classes boast scientific reasoning as a main learning outcome, but does reasoning transfer between disciplines and thus benefit the person as a whole? We hypothesized that most, if not all, disciplines use HD reasoning. In addition, we hypothesized that because this skill is context independent …

Do Differently Managed Dairy Cows With Production-Related Metabolic Diseases Have Similar Protein And Fat Species In Colostrum And Milk?, Holly Martin, Dr. Beverly Roeder

Journal of Undergraduate Research

Production-related metabolic diseases (PRMDs) in dairy cows threaten the livelihood of the animals as well as the productivity and success of the dairy industry. Because dairy cows supply an important food source for humans, understanding, managing, and preventing these diseases protects this source. Management and prevention of these diseases would increase production and profit margins for the dairy industry.

Confirmation Of Potential Cyprinid Hosts For A State Threatened Freshwater Mussel Of East Texas, Erin P. Bertram

Biology Theses

Previously suggested Cyprinid host-fish for the state threatened Texas pigtoe (Fusconaia askewi) and Louisiana pigtoe (Pleurobema riddelli) of East Texas were investigated. The Red shiner, Blacktail shiner, and Bullhead minnow were targeted as potential hosts. The viability of these fish as hosts was tested by collecting them from three different rivers of East Texas and housing them in an Aquatic Habitat Tank System (AHAB) to allow the natural development and release of glochidia or juvenile mussels that were attached to the fish in the wild. Juvenile mussels were molecularly identified with amplification of mitochondrial DNA (mtDNA) using the ND1 gene. …

Manipulation Of The Solenopsis Invicta Virus-1 By Rna Interference In The Red Imported Fire Ant Solenopsis Invicta (Hymenoptera: Formicidae), And Examination Of Differences In Resulting Micro Rna Expression, Patrick Rydzak

Biology Theses

The red imported fire ant, Solenopsis invicta (Hymenoptera, Formicidae), is an invasive ant species from South America to the United States and is responsible for approximately six billion dollars (U.S.) every year. In 2004, as part of an effort to find a self-sustained, long term biological control agent against S. invicta, researchers discovered the first ever known virus to infect Solenopsis sp., the Solenopsis invicta virus-1 (SINV-1). RNA interference (RNAi) is a known regulator of biological systems in insects and was used in this study to manipulate viral titers of SINV-1 in individual S. invicta workers and whole S. invicta …

Synthesis And Biological Evaluation Of N-Alkoxyphenyl-3-Hydroxynaphthalene-2-Carboxanilides, Eoghan Nevin, Aidan Coffey, Jim O'Mahony, Et. Al.

Department of Biological Sciences Publications

A series of fifteen new N-alkoxyphenylanilides of 3-hydroxynaphthalene-2-carboxylic acid was prepared and characterized. Primary in vitro screening of the synthesized compounds was performed against Staphylococcus aureus, three methicillin-resistant S. aureus strains, Mycobacterium tuberculosis H37Ra and M. avium subsp. paratuberculosis. Some of the tested compounds showed antibacterial and antimycobacterial activity against the tested strains comparable with or higher than that of the standards ampicillin or rifampicin. 3-Hydroxy-N-(2-propoxyphenyl)naphthalene-2-carboxamide and N-[2-(but-2-yloxy)-phenyl]-3-hydroxynaphthalene-2-carboxamide had MIC = 12 µM against all methicillin-resistant S. aureus strains; thus their activity is 4-fold higher than that of ampicillin. The second mentioned compound as well as 3-hydroxy-N-[3-(prop-2-yloxy)phenyl]-naphthalene-2-carboxamide had MICs = …

Did Tardigrades Survive The Last Glacial Maximum In Antarctica?, Caj Johansson, Byron Adams

Journal of Undergraduate Research

The current belief of the majority of glaciologists is that during the last glacial maximum (LGM), 17,000-22000 years ago, the entire Antarctic continent was completely covered in ice (Convey, 2008). This would mean that all terrestrial organisms would have died out during this time, essentially leaving Antarctica without life. If this hypothesis is true then all terrestrial organisms found currently in Antarctica arrived after the LGM (the past 12-18,000 years), a very short time frame for speciation to occur. Tardigrades are microscopic terrestrial animals that are most closely related to Arthropods and Nematodes (Edgecombe, 2011 but see also Longhorn, 2007). …

Nuclear Pore Protein Nup211 Is Essential For Cell Cycle Progression And Cell Shape Maintenance In Fission Yeast, Ayisha R. Sookdeo

Dissertations, Theses, and Capstone Projects

The nuclear pore complexes (NPCs) are large multi-protein channels that traverse the nuclear envelope and mediate nucleo-cytoplasmic transport. Proteomic studies have revealed that NPCs are composed of about thirty different proteins called nucleoporins. I explored a particular nucleoporin, Nup211, in fission yeast. nup211 is an essential gene; its deletion is lethal and causes morphological defects. In this study, I characterized the cell morphological defects caused by the down-regulation of Nup211 and found that restoring the N-terminal domain of Nup211 was sufficient to rescue the lethal phenotype and partially suppress the morphological defects. Additionally, I investigated the role Nup211 plays in …

Testing Visual Ecology Hypotheses In Avian Brood Parasite-Host Systems: The Role Of Uv-Light Perception And Egg-Nest Contrast In Foreign Egg Rejection, Zachary Aidala

Dissertations, Theses, and Capstone Projects

Color signals are highly important features of animal communication systems, particularly among birds, which possess exquisitely complex visual perception systems. Birds possess tetrachromatic vision, and some species are sensitive to ultraviolet (UV) wavelengths. Because human and avian visual systems dramatically differ (i.e. humans are not sensitive to UV wavelengths), biologically relevant sensory models are necessary to accurately assess the function of avian color signals. In this dissertation, I primarily use brood parasite-host interactions as a model for studying the behavioral function of avian-perceivable visual stimuli. In Chapter 1, I review the importance of employing biologically relevant sensory-perceptual visual models when …

Control Of Cell Cycle Progression By Mtor, Amrita Chatterjee

Dissertations, Theses, and Capstone Projects

Over the past few years it has become apparent that cancer cells require the activation of a set of intra-cellular signals that promote cell cycle progression and survival. One of the most common survival signals activated in human cancers is mediated by mTOR -the mammalian target of rapamycin. mTOR is a critical nutrient and energy sensor in cells that lets the cell know that there is sufficient material available for a cell to double its mass and divide. mTOR causes the phosphorylation of downstream targets ribosomal subunit S6 kinase and eukaryotic initiation factor 4E (eIF4E) binding protein-1 (4E-BP1), which promotes …

Phylogeography Of Southeast Asian Flying Foxes (Chiroptera: Pteropodidae: Pteropus), Susan Man Shu Tsang

Dissertations, Theses, and Capstone Projects

Flying foxes (Pteropus) are a genus of Old World fruit bats that are important seed dispersers and pollinators for plants native to the 200,000+ islands in Southeast Asia, yet they are some of the most poorly known bats in the world. They comprise some of the largest known bat species, and are morphologically relatively conserved on the genus level. Pteropus is the most species-rich genus within Pteropodidae, though the origin for this diversity remains incompletely understood. In Chapter 1, I discuss the importance of Pteropus to the ecosystem and as reservoir hosts. In Chapter 2, a molecular phylogeny is presented …

The Effects Of Sustained, High-Velocity Exercise On Gene Expression In California Yellowtail (Seriola Lalandi), Kelli Hatter

Undergraduate Honors Theses

California Yellowtail muscle fibers have been observed to exhibit two drastically different development patterns resulting from the speeds at which they are exercised. When fish are exercised at a moderate rate their epaxial fast-twitch muscle fibers grow in diameter—hypertrophy; when they are exercised at a fast speed, more new epaxial fast-twitch muscle fibers are produced—hyperplasia. To determine the underlying reason for this difference in muscle development, my summer research project and honors thesis exercised fish at: fast, moderate, and control speeds for a sustained amount of time to determine what is happening on a cellular level to cause the observed …

Mechanisms Of Axial Polarity Modification During Postembryonic Development Of The Basal Bilaterian Convolutriloba Macropyga, Laura N. Crowther, James M. Sikes Ph.D.

Undergraduate Honors Theses

Acoel flatworms have varied modes of asexual reproduction that involve dramatic postembryonic modification of their anterior-posterior (AP) axis. The acoel species Convolutriloba macropyga reproduces through a reversed polarity budding process in which offspring develop from two posterior budding sites with a complete reversal of the AP axis compared to the parent. Reversed polarity budding is preceded by the development of a zone of tissue with disorganized musculature that is incapable of regeneration, suggesting a transient loss of axis polarity at each budding site. For this reason, these tissues are titled the polarity transition zone (PTZ). While this alteration of existing …

Riparian Restoration, Success Criteria, And Application To The Bart Sabrecat Creek Riparian Restoration Project In Fremont, California, Jody D. Fessler

Master's Projects and Capstones

Riparian restoration is a complex, inter-disciplinary field that is open to interpretation by its practitioners. This research reviews some of the critical elements of riparian restoration, including the watershed, surrounding land use, adjacent habitat, and riparian buffers; special-status species, endangered species, and habitat type and structure; invasive species control; water quality and hydrology; and soil and soil microbial communities. Three methods of evaluating restoration projects are discussed, including the Society for Ecological Restoration International Primer, the California Department of Fish and Wildlife (CDFW) California Salmonid Stream Habitat Restoration Manual and CDFW permitting, and the California Rapid Assessment Method. Finally, the …

Transcriptional Regulation By Dax-1 In Pluripotent And Differentiated Cells, Alexandra C. Maramba

Master's Theses

DAX-1, an orphan nuclear hormone receptor, acts mainly as a repressor through transcriptional protein complexes. Its unique structure and specific expression raises questions as to what its precise interactions are and how it mediates its repressive function. While it is known to play a role in sexual development and adrenal insufficiency, expression in certain types of cancer suggests additional functions and interactions. Knock in of DAX-1 into a low-DAX-1 expressing cancer cell line has been previously observed to increase apoptosis, while, inversely, down in a high-DAX-1 expressing cancer cell line shows a decrease in apoptosis. Target genes that belong to …

Genome-Wide Association Study Of Cerebrospinal Fluid Prostatic Acid Phosphatase Levels, Lyndsay A. Staley, John S. K. Kauwe

Journal of Undergraduate Research

In genome wide association studies (GWAS), the use of endophenotypes, or intermediate traits, have been found to provide novel insights into understanding the genetics of complex human disease and the pathways and proteins associated with them. Cerebrospinal fluid (CSF) has been shown to contain analytes that may provide promising insight into disease pathways that may not be identified using blood or other biological fluids. The analytes were selected from the Rules Based Medicine, Inc. (RBM) (Austin, TX) Human Discovery Panel 1.0, which includes a range of signaling, structural, and trafficking proteins that have previously shown relationships with human disease pathology. …

Diverging Predation Environment Between Two Sister Species Of Live Bearing Fish: Boldness, Activity, And Exploration., David Money, Jerald B. Johnson

Journal of Undergraduate Research

Our study takes advantage of a unique species pair within Brachyrhaphis, Brachyrhaphis roseni and B. terrabensis,, which provides insight into how divergence proceeds once speciation is complete. Brachyrhaphis roseni and B. terrabensis are sister species that occur in the same river drainages throughout northwestern Panama. A similar pattern occurs between these two species as occurs within other species of Brachyrhaphis, such that B. roseni lives in the presence of ambush predators (e.g., Hoplias microlepis and Gobiomorus dormitor) in lower elevation streams, and B. terrabensis, lives in largely predator-free, higher elevation streams. Therefore, using B. roseni and …

Effects Of Phosphorous Availability On Nematode Growth And Morphology In Vitro, Ammon Perkes, Byron Adams

Journal of Undergraduate Research

The Growth Rate Hypothesis (GRH) proposes that since high-growth protein synthesis in organisms requires phosphorous-rich rRNA, growth is correlated with and dependent on phosphorous availability to said organisms. The limitation imposed by phosphorus availability thus becomes a selective factor that drives evolution. (Elser et al, 2002). This project seeks to test the GRH in vitro using Plectus murrayi a species of Antarctic nematodes. Previous research in the McMurdo Dry Valleys in Antarctica found a correlation between life history in P. murrayi, including growth, and phosphorous availability. (Adhikari et al, in prep). We seek to replicate this difference in vitro …

Testing The Validity Of The Lctsr 12-Point Grading System, Ted Poirczynski, Jamie Jensen

Journal of Undergraduate Research

The Lawson Classroom Test of Scientific Reasoning (LCTSR) is a 24-question, multiple-choice test designed to assess students’ scientific reasoning ability. The test consists of 12 scenarios, each of which focuses on testing a specific reasoning pattern. Each scenario is followed by two questions; the first question assesses a student’s ability to apply the specific reasoning pattern being tested, whereas the second asks the student to explain the reasoning behind his or her response to the first question. This dual question approach is designed to verify that students are employing the correct reasoning patterns to reach the correct answers.

Potential Use Of Basil And Lemongrass Essential Oils Against Human Bacterial Pathogens, Mary Riley, Jeremy Martell, Maryann Herman

Biology Faculty/Staff Publications

The increasing prevalence of multi-drug resistant bacterial infections fuels a continuing need to find effective antimicrobial agents. Basil (Ocimum basilicum) and lemongrass (Cymbopogon citratus) essential oil activity against sixteen true and opportunistic human pathogenic bacterial strains was tested, including: S. aureus, S. epidermidis, B. cereus and E. aerogenes. Inhibition of microbial growth by both essential oils was determined using a Kirby-Bauer disc diffusion assay and results compared to common antibiotics. Results indicated that both essential oils possess antimicrobial compounds against select bacterial strains. Our data support phytomedicine as a plausible option to combat antibiotic …

Multi-Year Analysis Of Microbial Populations In The Rochester-Lake Ontario Embayment, Daniel Zimmerman, Laura Moore, Joseph Dora, J. A. Concha, N. Raqueno, Maryann Herman, Fernando Ontiveros

Biology Faculty/Staff Publications

The composition of freshwater bacterial populations is affected by a wide variety of factors. Temperature, acidity, organic matter, and environmental pollutants like industrial chemicals and antibiotics are a few examples. The impact that bodies of freshwater have on human activity and the wider ecosystem warrants the systematic identification of microbial flora, and in particular of species known to be pathogenic in plants and animals. In order to achieve the long term goal of using satellite imagery to predict the occurrence of specific bacterial species, our team is in the process of creating a multi-location, multi-year microbial flora database for the …

Firefly Evolution And Signaling Systems, Michael Swindle, Michael Whiting

Journal of Undergraduate Research

The goal of this project was to expand and clarify the relationships between the various subfamilies of fireflies. Lampyridae (the scientific name for fireflies) is a family within the beetle order, Coleoptera, and include about 2000 species across the world. Lampyridae is currently recognized as having nine separate subfamilies, but the relationships between these groups are unclear. This project was meant to determine clear relationsips between these groups and further determine the location of key species, such as Pterotus obscuripennis, whose phylogenetic position has remained uncertain after many attempts to place it firmly within a specific subfamily. However, this …

Expressing And Characterizing Mechanosensitive Channels In Xenopus Oocytes, Grigory Maksaev, Elizabeth S. Haswell

Biology Faculty Research

The oocytes of the African clawed frog (Xenopus laevis) comprise one of the most widely used membrane protein expression systems. While frequently used for studies of transporters and ion channels, the application of this system to the study of mechanosensitive ion channels has been overlooked, perhaps due to a relative abundance of native expression systems. Recent advances, however, have illustrated the advantages of the oocyte system for studying plant and bacterial mechanosensitive channels. Here we describe in detail the methods used for heterologous expression and characterization of bacterial and plant mechanosensitive channels in Xenopus oocytes.

Genetic Basis For Rheumatic Heart Disease In High Risk Families In Western Samoa, Eleni Tukuafu, John S.K. Kauwe

Journal of Undergraduate Research

Rheumatic heart disease (RHD) is the most common form of acquired cardiovascular disease among children worldwide and results in 233,000 deaths annually. Although genetic and environmental factors are known to contribute to RHD, not much is known about genes that increase susceptibility. RHD results from untreated rheumatic fever (RF). RF develops when a Streptococcus pyogenes infection, such as streptococcal pharyngitis, goes untreated. About 34% of children with an untreated Streptococcus pyogenes infection develop rheumatic fever. Only 1/3 of those children acquire RHD¹. This suggests that there is a genetic basis for why some children are prone to the …

Nsf Grant Helps Preserve Parasite Collections [Press Release], Scott Gardner, Leslie Reed

Scott L. Gardner Publications

The National Science Foundation has awarded a $500,000 grant that will allow four major parasite collections to be digitized. The collections are part of UNL's Harold W. Manter Laboratory of Parasitology. Scott Gardner (pictured) is the curator and director of the Manter Laboratory.

A $500,000 grant from the National Science Foundation will allow the Harold W. Manter Laboratory of Parasitology to digitally preserve four major collections of parasite specimens donated to the University of Nebraska-Lincoln during the past five years.

Restoring Semi-Arid Thornscrub Forests: Seeding Growth And Survival In Response To Shelter Tubes, Grass-Specific Herbicide, And Herbivore Exclosures, Krysten Noelle Dick

Theses and Dissertations - UTB/UTPA

This thesis is a compilation of two studies, which I have called ‘chapters.’ The first chapter, “Restoring Semi-Arid Thornscrub Forests: Seedling Growth and Survival in Response to Shelter Tubes, Grass-Specific Herbicide, and Herbivore Exclosures,” was a large study involving multiple techniques used to improve revegetation success, and the second chapter, “Shelter Tube Effects on Microclimate and Seedling Growth in Semi-arid Thornscrub Forests,” focused on the use of shelter tubes in semi-arid forest revegetation. These studies were concurrent evaluations of restoration efforts in thornscrub forests of South Texas. Revegetation is a common effort used to restore forest ecosystems that have been …

Detection Of Hcmv Viral Il-10 (Vil-10) In Healthy Blood Donors, Vivian P. Young

Master's Theses

Human cytomegalovirus (HCMV) is widespread in the general population and can establish lifelong latency with expression of a limited subset of viral genes. The UL111A gene is expressed during both lytic and latent infection, and at least two protein isoforms are produced. During lytic infection, the full length transcript yields cmvIL-10, a potent immunosuppressive viral ortholog of human IL-10 (hIL-10). Alternative splicing of the UL111A transcript yields a truncated protein, LAcmvIL-10, which is expressed during both lytic and latent infection but with a limited range of immunosuppressive functions. The two viral cytokines, collectively termed viral IL-10 (vIL-10), are identical in …

Climate-Driven Change In Himalayan Rhododendron Phenology, Robert Evan Hart

Dissertations

Phenology – the seasonal timing of life-history events – is a critical dimension of natural history. It is also one of the earliest and most noticeable traits by which organisms respond to climate change. However, these responses are complex, and only beginning to be understood, especially in the montane and alpine environments that are among the ecosystems most vulnerable to climate change. Drawing from diverse data sets and employing multiple methodologies, I examined how climate affects phenology in Himalayan Rhododendron spp. Comprehensively monitoring flowering phenology over gradients of season and elevation on Mt. Yulong, China – home to a diverse …