Biology Commons^™

Ecology Postdocs In Academia: Primary Concerns And Possible Solutions, A. K. Shaw, D. E. Stanton, S. R. Supp, A. Budden, S. Eby, P. L. Reynolds, R. Salguero-Gómez, D. R. Scholes, Naupaka B. Zimmerman

Biology Faculty Publications

No abstract provided.

Connecting People And Ideas From Around The World: Global Innovation Platforms For Next-Generation Ecology And Beyond, P. Søgaard Jørgensen, F. Barraquand, V. Bonhomme, T. J. Curran, E. Cieraad, T. G. Ezard, L. Gherardi, R. A. Hayes, T. Poisot, R. Salguero-Gómez, Lucía Desoto, B. Swartz, J. M. Talbot, B. Wee, Naupaka B. Zimmerman

Biology Faculty Publications

We present a case for using Global Community Innovation Platforms (GCIPs), an approach to improve innovation and knowledge exchange in international scientific communities through a common and open online infrastructure. We highlight the value of GCIPs by focusing on recent efforts targeting the ecological sciences, where GCIPs are of high relevance given the urgent need for interdisciplinary, geographical, and cross-sector collaboration to cope with growing challenges to the environment as well as the scientific community itself. Amidst the emergence of new international institutions, organizations, and meetings, GCIPs provide a stable international infrastructure for rapid and long-term coordination that can be …

The Tao Of Open Science For Ecology, S. E. Hampton, S. S. Anderson, S. C. Bagby, C. Gries, X. Han, E. M. Hart, M. B. Jones, W. C. Lenhardt, A. Macdonald, W. K. Michener, J. Mudge, A. Pourmokhtarian, M. P. Schildhauer, K. H. Woo, Naupaka B. Zimmerman

Biology Faculty Publications

The field of ecology is poised to take advantage of emerging technologies that facilitate the gathering, analyzing, and sharing of data, methods, and results. The concept of transparency at all stages of the research process, coupled with free and open access to data, code, and papers, constitutes “open science.” Despite the many benefits of an open approach to science, a number of barriers to entry exist that may prevent researchers from embracing openness in their own work. Here we describe several key shifts in mindset that underpin the transition to more open science. These shifts in mindset include thinking about …

Human Cytomegalovirus Interleukin-10 Promotes Proliferation And Migration Of Mcf-7 Breast Cancer Cells, Robin K. Bishop, Cendy A. Valle Oseguera, Juliet Spencer

Biology Faculty Publications

Breast cancer is the most common malignancy affecting women worldwide. While a small fraction of breast cancers have a hereditary component, environmental and behavioral factors also impact the development of cancer. Human cytomegalovirus (HCMV) is a member of the Herpesviridae family that is widespread in the general population and has been linked to several forms of cancer. While HCMV DNA has been found in some breast cancer tissue specimens, we wanted to investigate whether a secreted viral cytokine might have an effect on cancerous or even pre-cancerous cells. HCMV encodes an ortholog of the human cellular cytokine interleukin-10 (IL-10). The …

Exercise At Depth Alters Bradycardia And Incidence Of Cardiac Anomalies In Deep-Diving Marine Mammals, T. M. Williams, L. A. Fuiman, T. Kendall, P. Berry, B. Richter, S. R. Noren, Nicole Thometz, M. J. Shattock, E. Farrell, A. M. Stamper, R. W. Davis

Biology Faculty Publications

Unlike their terrestrial ancestors, marine mammals routinely confront extreme physiological and physical challenges while breath-holding and pursuing prey at depth. To determine how cetaceans and pinnipeds accomplish deep-sea chases, we deployed animal-borne instruments that recorded high-resolution electrocardiograms, behaviour and flipper accelerations of bottlenose dolphins (Tursiops truncatus) and Weddell seals (Leptonychotes weddellii) diving from the surface to 4200 m. Here we report that both exercise and depth alter the bradycardia associated with the dive response, with the greatest impacts at depths inducing lung collapse. Unexpectedly, cardiac arrhythmias occurred in 473% of deep, aerobic dives, which we attribute to the interplay between …

Influence Of Stress On Substance Use: Age-Associated Variability In Molecular And Behavioral Outcomes, Christina Elizabeth D'Arcy

Open Access Theses & Dissertations

Stress introduces a number of neurological and neurohormonal changes in response to a number of stimuli, and can influence our relationship to rewarding experiences. As such, it holds the potential of promoting vulnerability to addiction to substances such as methamphetamine. While methamphetamine use and abuse has been steadily declining, the fact remains that it and closely related drugs are used in the treatment of conditions such as narcolepsy, weight loss, and attention deficit disorder. In an effort to develop better patient assessment metrics to aid the physician in deciding a course of treatment, we ask the following questions: Do chronic …

The Role Of P62/Imp2 In Breast Cancer Progression, Yang Li

Open Access Theses & Dissertations

Breast cancer is one of the most common types of cancer in women. In most patients with breast cancer, it is not the primary tumor that leads to death, but rather metastatic tumors. Once breast cancer spreads to other organs in the body, the disease becomes almost incurable. It is estimated that about 20% to 50% of patients diagnosed with primary breast cancer will develop metastatic disease. Considering this, it is important to understand the molecular and cellular mechanisms behind metastatic breast cancer and to identify new proteins that regulate the metastatic process. These proteins may be used as targets …

Development Of A Yeast-Based Assay For The Functional Characterization Of Fkbp52/ Î?-Catenin Interactions With The Androgen Receptor, Theresa Anne Rodriguez

Open Access Theses & Dissertations

Prostate cancer development is uniquely dependent on the androgen receptorâ??s (ARâ??s) transcriptional regulation in response to hormone binding. Current therapies directly target AR acting as an antagonist at androgen binding sites. However, once androgen-dependence is lost, meaning the patient has progressed into a late-stage hormone resistant phenotype, all current treatments are essentially ineffective. Utilizing the yeast strain Saccharomyces cerevisiae we are capable of creating a model system that allows for the exogenous expression of AR while still retaining the chaperone components needed for this steroid receptor complex. The 52kDa FK506 binding protein (FKBP52) has been shown to be an important …

Serine Palmitoyltransferase And Giardial Encystation, Monica Delgado

Open Access Theses & Dissertations

Giardia lamblia is a flagellated protozoan parasite responsible for chronic diarrheal disease in humans and animals. Giardia has minimal lipid synThesis machinery and the majority of its lipids are obtained from the small intestine of humans where the trophozoites colonize. Recent results from our laboratory indicate that sphingolipids (SLs) are also important for Giardia and they regulate encystation and cyst production. Only five SL genes are present in this parasite and they are differentially regulated during encystation. In my studies, I found that giardial serine palmitoyltransferase (gSPT) activity is upregulated in encysting cells, suggesting it might play an important role …

Expression And Functional Analysis Of Lipids And Glycolipids From The Mammal-Dwelling Stages Of Trypanosoma Cruzi, Felipe Gazos Lopes

Open Access Theses & Dissertations

Trypanosoma cruzi is the causative agent of the life-threatening Chagas disease, in which increased platelet aggregation related to myocarditis is observed. Platelet-activating factor (PAF) is a potent intercellular lipid mediator and second messenger that exerts its activity through a PAF-specific receptor (PAFR). Previous data from our group suggested that T. cruzi synthesizes a phospholipid with PAF-like activity. The structure of T. cruzi PAF-like molecule, however, remains elusive. Here, we have purified and structurally characterized the putative T. cruzi PAF-like molecule by electrospray ionization-tandem mass spectrometry (ESI-MS/MS). Our ESI-MS/MS data demonstrated that the T. cruzi PAF-like molecule is actually a lysophosphatidylcholine …

Introduction Effort, Climate Matching And Species Traits As Predictors Of Global Establishment Success In Non-Native Reptiles, Peter J. Mahoney, Karen H. Beard, Andrew M. Durso, Aimee G. Tallian, Arin Lexine Long, Ryan J. Kindermann, Nicole E. Nolan, Daniel Kinka, Harrison E. Mohn

Biology Faculty Publications

Non-native reptiles are often detrimental to native communities and ecosystems and can be extremely difficult to manage once established. Thus, there is considerable interest in predicting the likelihood of establishment of nonnative reptiles. We assessed three hypotheses describing possible factors contributing to the successful establishment of introduced reptiles in an effort to better identify potential invaders.

Learning To Love Leftovers: Using By-Catch To Expand Our Knowledge In Entomology, John M. Stark, Ricardo A. Ramirez

Biology Faculty Publications

Each year, federal and state agencies, including universities, conduct field surveys for invasive species in an attempt to detect new introductions early in the invasion process. National surveys for invasive insects are administered and coordinated by the United States Department of Agriculture's Animal and Plant Health Inspection Service-Plant Protection and Quarantine (USDA-APHIS-PPQ) and the Forest Service through Farm Bill § 10007 ( www.aphis.usda.gov ), the Cooperative Agricultural Pest Survey (CAPS) (caps.ceris.purdue.edu), and Early Detection Rapid Response (EDRR) ( www.fs.fed.us/invasivespecies ) programs. Thousands of traps are placed annually and monitored usually every one to two weeks throughout the U.S., mostly during …

Amyloid Precursor Protein (App) Regulates G0/G1 Transition And Cell Growth, Anna Sobol

Dissertations

Non-small cell lung cancer (NSCLC) originates in the epithelia of the lung and persists as the leading cause of cancer-related death in the United States. Many studies have shown that hypoxia is prevalent in NSCLC tissue and negatively influences treatment outcome.

In previous studies, we investigated the efficacy of gamma-secretase inhibitor (GSI) treatment in an orthotopic mouse NSCLC model. Analysis of hypoxic areas of tumors from GSI treated animals showed reappearance of 4E-BP1 phosphorylation at threonine 37/46 residues (T37/46). 4E-BP1 phosphorylation is primarily mediated by mechanistic target of rapamycin complex 1 (mTORC-1), a major cell integrator of growth factors stimulation, …

Survey Of Viral Populations Within Lake Michigan Nearshore Waters At Four Chicago Area Beaches, Emily Sible, Alexandria Cooper, Kema Malki, Katherine Bruder, Siobhan C. Watkins, Yuriy Fofanov, Catherine Putonti

Bioinformatics Faculty Publications

In comparison to the oceans, freshwater environments represent a more diverse community of microorganisms, exhibiting comparatively high levels of variability both temporally and spatially Maranger and Bird, Microb. Ecol. 31 (1996) 141–151. This level of variability is likely to extend to the world of viruses as well, in particular bacteria-infecting viruses (bacteriophages). Phages are known to influence bacterial diversity, and therefore key processes, in environmental niches across the globe Clokie et al., Bacteriophage 1 (2011) 31–45; Jacquet et al., Adv. Ocean Limn. 1 (2010) 97–141; Wilhelm and Suttle, Bioscience 49 (1999) 781–788; Bratback et al., Microb. Ecol. 28 (1994) 209–221. …

Survey Of Microbial Populations Within Lake Michigan Nearshore Waters At Two Chicago Public Beaches, Kema Malki, Katherine Bruder, Catherine Putonti

Bioinformatics Faculty Publications

Lake Michigan is a critical resource for the residents of Chicago, providing drinking water to its 9+ million area residents. Along Chicago׳s 26 miles of public beaches the populous urban environment and this freshwater environment meet. While city-led monitoring initiatives investigate pathogenic bacteria in these nearshore waters, very little is known about other microbial species present. We collected surface water samples from two Chicago public beaches – Montrose Beach and 57th Street Beach – every ten days from June 5 through August 4, 2013 as well as once in early Fall (October 4, 2013). Sixteen bacterial communities in total were …

The Brain Geography Mini-Course: A Neuroscience Outreach Effort, Mark Albert

Computer Science: Faculty Publications and Other Works

The way we experience the world - how we go between sensing, thinking, and acting - is in some ways no more of a mystery than understanding how a computer works. Brains are quite complicated and we may never understand the details, but what we know in general can be very interesting. In this mini-course, we will learn how we think. When we see, hear, smell, touch, taste, remember, rehearse, fear something, understand or produce language, move our bodies...specific parts of the brain are used. Students will get a rough introduction to each part and how they interact. This will …

Diet Of Larval Ambystoma Altamiranoi From Llano De Los Axolotes, Mexico, J. A. Lemos-Espinal, Geoffrey R. Smith, G. A. Woolrich-Pina

Faculty Publications

No abstract provided.

Amphibians And Reptiles Of The State Of Hidalgo, Mexico, J. A. Lemos-Espinal, Geoffrey R. Smith

Faculty Publications

No abstract provided.

Thermal Ecology, Sexual Dimorphism, And Diet Of Xenosaurus Tzacualtipantecus From Hidalgo, Mexico, J. Garcia-Rico, A. Diaz De La Vega-Pere, Geoffrey R. Smith, J. A. Lemos-Espinal, G. A. Woolrich-Pina

Faculty Publications

No abstract provided.

Multi-Platform Arabinoxylan Scaffolds As Potential Wound Dressing Materials, Donald C. Aduba Jr

Theses and Dissertations

Biopolymers are becoming more attractive as advanced wound dressings because of their naturally derived origin, abundance, low cost and high compatibility with the wound environment. Arabinoxylan (AX) is a class of polysaccharide polymers derived from cereal grains that are primarily used in food products and cosmetic additives. Its application as a wound dressing material has yet to be realized. In this two-pronged project, arabinoxylan ferulate (AXF) was fabricated into electrospun fibers and gel foams to be evaluated as platforms for wound dressing materials. In the first study, AXF was electrospun with varying amounts of gelatin. In the second study, AXF …

Occurrence Of The Asiatic Weatherfish, Misgurnus Anguillicaudatus (Cantor, 1842), In Alabama, Usa, Lindsay Michelle White, Mark Edward Meade

Research, Publications & Creative Work

The Asiatic weatherfish, Misgurnus anguillicaudatus, is a generalist species that has invaded numerous physiographic niches worldwide. Asiatic weatherfish populations have been observed to compete with native fish populations in Hawaii and are of major concern in Australia due to the concomitant introduction of an exotic parasite. Asiatic weatherfish populations have been observed in 16 of the contiguous United States (US) since the 1940s. Alabama is the most recent US state to report sustaining Asiatic weatherfish populations. Asiatic weatherfish were first observed by local fishermen in 2000, but reported established in Alabama in 2009 and, more recently, in 2012. From 2013 …

Predicting Effects Of Ocean Warming, Acidification, And Water Quality On Chesapeake Region Eelgrass, Richard C. Zimmerman, Victoria J. Hill, Charles L. Gallegos

OES Faculty Publications

Although environmental requirements of seagrasses have been studied for years, reliable metrics for predicting their response to current or future conditions remain elusive. Eelgrass (Zostera marina L.) populations of the Chesapeake region lie near the southern limit of their range in the Western North Atlantic, exposing them to increasing thermal stress as the climate warms. However, CO₂ stimulated photosynthesis may offset some of the negative effects of temperature stress. The combined effects of temperature, CO₂, and light availability controlled by water quality and epiphytes were explored using GrassLight, a bio-optical model that provided a predictive …

Prothonotary Warbler Nestling Diet And Growth In Response To Variation In Aquatic And Terrestrial Food Availability, Jenna C. Dodson

Theses and Dissertations

Food supply has been suggested as the main determinant of reproductive success in birds. Riparian species can take advantage of seasonal pulses of both terrestrial and aquatic prey, though aquatic resources are often overlooked in studies of diet and reproductive ecology. This study investigates the flux of both aquatic (mayfly) and terrestrial (caterpillar) prey resources and nestling diet of the Prothonotary Warbler throughout the breeding season in two eastern Virginia sites. One site had significantly higher aquatic prey (mayfly) availability. Nestling diet was generally reflective of prey availability, and nestlings grew faster at the site with high aquatic prey availability. …

Type I Interferons Link Viral Infection To Enhanced Epithelial Turnover And Repair, Lulu Sun, Hiroyuki Miyoshi, Sofia Origanti, Timothy J. Nice, Alexandra C. Barger, Nicholas A. Manieri, Leslie A. Fogel, Anthony R. French, David Piwnica-Worms, Helen Piwnica-Worms, Herbert W. Virgin, Deborah J. Lenschow, Thaddeus S. Stappenbeck

Biological Sciences Faculty Research and Publications

The host immune system functions constantly to maintain chronic commensal and pathogenic organisms in check. The consequences of these immune responses on host physiology are as yet unexplored, and may have long-term implications in health and disease. We show that chronic viral infection increases epithelial turnover in multiple tissues, and the antiviral cytokines type I interferons (IFNs) mediate this response. Using a murine model with persistently elevated type I IFNs in the absence of exogenous viral infection, the Irgm1^−/− mouse, we demonstrate that type I IFNs act through nonepithelial cells, including macrophages, to promote increased epithelial turnover and wound …

Different Sites Of Alcohol Action In The Nmda Receptor Glun2a And Glun2b Subunits, Yulin Zhao, Hong Ren, Donard S. Dwyer, Robert W. Peoples

Biological Sciences Faculty Research and Publications

The NMDA receptor is a major target of alcohol action in the CNS, and recent behavioral and cellular studies have pointed to the importance of the GluN2B subunit in alcohol action. We and others have previously characterized four amino acid positions in the third and fourth membrane-associated (M) domains of the NMDA receptor GluN2A subunit that influence both ion channel gating and alcohol sensitivity. In this study, we found that substitution mutations at two of the four corresponding positions in the GluN2B subunit, F637 and G826, influence ethanol sensitivity and ion channel gating. Because position 826 contains a glycine residue …

Liana Competition With Tropical Trees Varies Seasonally But Not With Tree Species Identity, Leonor Álvarez-Cansino, Stefan A. Schnitzer, Joseph P. Reid, Jennifer S. Powers

Biological Sciences Faculty Research and Publications

Lianas in tropical forests compete intensely with trees for above‐ and belowground resources and limit tree growth and regeneration. Liana competition with adult canopy trees may be particularly strong, and, if lianas compete more intensely with some tree species than others, they may influence tree species composition. We performed the first systematic, large‐scale liana removal experiment to assess the competitive effects of lianas on multiple tropical tree species by measuring sap velocity and growth in a lowland tropical forest in Panama. Tree sap velocity increased 60% soon after liana removal compared to control trees, and tree diameter growth increased 25% …

The Ecology Of Lianas In Tropical Forests, Stefan A. Schnitzer

Biological Sciences Faculty Research and Publications

No abstract provided.

A Coxiella-Like Endosymbiont Is A Potential Vitamin Source For The Lone Star Tick, Todd A. Smith, Timothy Driscoll, Joseph J. Gillespie, Rahul Raghavan

Biology Faculty Publications and Presentations

Amblyomma americanum (Lone star tick) is an important disease vector in the United States. It transmits several human pathogens, including the agents of human monocytic ehrlichiosis, tularemia, and southern tick-associated rash illness. Blood-feeding insects (Class Insecta) depend on bacterial endosymbionts to provide vitamins and cofactors that are scarce in blood. It is unclear how this deficiency is compensated in ticks (Class Arachnida) that feed exclusively on mammalian blood. A bacterium related to Coxiella burnetii, the agent of human Q fever, has been observed previously within cells of A. americanum. Eliminating this bacterium (CLEAA, Coxiella-like endosymbiont of A. americanum) with antibiotics …

Maternal Stress, Breastmilk Igf-1, And Offspring Growth Among Breastfeeding Mothers-Infant Pairs In The Tampa Bay Area, Lauren Michelle Gottfredson

USF Tampa Graduate Theses and Dissertations

Background: Maternal stress during utero has been shown to have negative health consequences on the offspring, including low birth weight and increased risk of adult disease. Variation in breastmilk may act as an environmental cue of maternal stress and continue to program the infant during early life. This research aimed to explore the role of breastmilk on developmental programming of the infant. Specifically, to examine how breastmilk may act as a medium for the exposure of stress between the mother and the offspring, and see if variation in insulin like growth factor-1 (IGF-1) a potential mechanism for the relationship.

Methods: …

Structure-Function Analysis Of The Dna Damage Repair Complex Str In Saccharomyces Cerevisiae, Jessica Ashley Kennedy

USF Tampa Graduate Theses and Dissertations

The RecQ family of helicases has been termed the “Caretakers of the Genome,” and rightfully so. These proteins are highly conserved from bacteria to humans and have been implicated in functions from homologous recombinatorial repair to damage checkpoint response to telomere maintenance and more. Mutant genes of three of the human RecQ helicases lead to syndromes characterized by a high incidence of cancer, premature aging and early death. Despite their implications in several biological functions and importance to the integrity of the human genome and suppression of cancer, many aspects of the RecQ family structure and function remain unknown. To …