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2015

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Articles 1471 - 1500 of 1751

Full-Text Articles in Biology

Effects Of Atp And Atp Inhibitors On The P2x7 Receptor, Holly Marie Salzbrenner Jan 2015

Effects Of Atp And Atp Inhibitors On The P2x7 Receptor, Holly Marie Salzbrenner

Honors Program Theses

Extracellular ATP has been shown to increase the permeability of cells via the P2X7 receptor. This relationship could be important in understanding the physiology of the inflammation that occurs in bovine respiratory disease complex (BRDC). My research involved testing to see if this relationship between ATP and the P2X7 receptor is present. Then, potential inhibitors of ATP were tested so they can be used in further research involving ATP and BRDC. To test bovine cells, Mac-T cells were treated with ATP and incubated for various periods of time before Yo-Pro (a fluorescent molecule) was added. The percent change in fluorescence …

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Functional Analysis Of Drought-Resistant Genes In Barley Using Vigs, Natalie Catherine Martin Jan 2015

Functional Analysis Of Drought-Resistant Genes In Barley Using Vigs, Natalie Catherine Martin

Honors Program Theses

The following study analyzed the role of the gamma-aminobutyric acid transaminase (GABAT) gene in the response of barley plants to drought stress. Expression of GABAT in barley was suppressed using virus-induced gene silencing (VIGS). In VIGS, an RNA virus is used to replicate a small portion of the target RNA. In this study, barley stripe mosaic virus (BSMV), that carries a piece of the GABAT gene to infect barley, was used. As the virus replicates inside infected barley plants, it also makes RNA that is complementary to GABAT messenger RNA (mRNA) that is normally made by the host. The presence …

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The Effects Of Substrate Roughness In Air And Water On The Gecko Adhesive System, Amanda Palecek Jan 2015

The Effects Of Substrate Roughness In Air And Water On The Gecko Adhesive System, Amanda Palecek

Williams Honors College, Honors Research Projects

In an effort to better understand the fascinating gecko adhesive system, scientists have long tested the abilities of the gecko in controlled conditions that mimic the gecko’s native environment. The effects of humidity, environmental temperature, and exposure to water have all been tested. Roughness, however has not been studied in great detail. Surfaces of varying roughnesses are all over the gecko’s natural environment. We tested geckos on hydrophilic silicon carbide sandpapers of varying roughnesses in both air and water to attempt to better study the effects of roughness on gecko adhesion. When tested on the rougher (1 µm) surface, the …

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Characterization Of Two Biochars Derived From Horse Muck And Their Ability To Reduce Pathogen Transport In Soil, David Griffith Jan 2015

Characterization Of Two Biochars Derived From Horse Muck And Their Ability To Reduce Pathogen Transport In Soil, David Griffith

Theses and Dissertations--Biosystems and Agricultural Engineering

Biochars have been created and characterized from a variety livestock manure biomass including poultry, dairy, and swine. However, no research has been conducted on the physical and chemical makeup of biochar pyrolyzed from horse muck. Two horse muck derived biochars containing either straw (HS) or woodchip (HW) bedding were pyrolyzed at 700°C and characterized for their physical and chemical properties. Tests revealed both biochars had high alkalinity, moderate specific conductivity, and low surface area as compared to other biochars in the literature. HS contained more mineral structures than HW. Scanning electron microscopy presented differences in particle shape, size, and presence …

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Types Of Ampullariidae (Mollusca: Gastropoda) In The National Museum Of Natural History, Smithsonian Institution, With Lectotype Designations, Robert Cowie, Kenneth Hayes, Ellen Strong Jan 2015

Types Of Ampullariidae (Mollusca: Gastropoda) In The National Museum Of Natural History, Smithsonian Institution, With Lectotype Designations, Robert Cowie, Kenneth Hayes, Ellen Strong

Department of Biology Faculty Publications

Cowie, Robert H., Kenneth A. Hayes, and Ellen E. Strong. Types of Ampullariidae (Mollusca: Gastropoda) in the NationalMuseum of Natural History, Smithsonian Institution, with Lectotype Designations. Smithsonian Contributions to Zoology, number 645, iv + 13 pages, 1 figure, 2015.—The caenogastropod family Ampullariidae Gray, 1824, is a family of freshwater snails predominantly distributed in humid tropical and subtropical habitats in Africa, South and Central America, and Asia. The family includes the largest of all freshwater snails and frequently constitutes a major portion of the native freshwater malacofauna of these regions. Ampullariid taxonomy is confused primarily because most species were described on …

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Characterization Of Cp43 And Pif1 Gwas Selected Candidate Genes In Medicago Truncatula And Their Contribution To Rhizobia Symbiosis And Phenotypes Of Interest Using An Hprna Based Rnai Platform, Nathaniel Henning Jan 2015

Characterization Of Cp43 And Pif1 Gwas Selected Candidate Genes In Medicago Truncatula And Their Contribution To Rhizobia Symbiosis And Phenotypes Of Interest Using An Hprna Based Rnai Platform, Nathaniel Henning

Departmental Honors Projects

Medicago truncatula is a model organism used to study the symbiotic relationship between plants and nitrogen fixing soil bacteria of the genus rhizobia. By studying the genes that are involved in this symbiosis we can accomplish the goal of transplanting the symbiotic relationship into agriculture staples (e.g. corn, wheat, etc.) that do not utilize similar symbiotic relationships with nitrogen fixers. This would help to reduce our reliance on nitrogen-based fertilizers. In order to identify plant genes that might be important for establishing and regulating optimal nitrogen-fixing symbioses, a large-scale Genome Wide Association Study (GWAS) was performed. This study identified …

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Effect Of Adjuvant And Neoadjuvant Anti-Telomerase With Anthracycline Based Chemotherapy On Triple Negative Breast Cancer Cells, Luke T. Pardy Jan 2015

Effect Of Adjuvant And Neoadjuvant Anti-Telomerase With Anthracycline Based Chemotherapy On Triple Negative Breast Cancer Cells, Luke T. Pardy

Student Summer Scholars Manuscripts

Breast cancer is the second leading cause of cancer related death in women in the US. In addition, 20% of all breast cancer cases in the U.S. are from the subtype known as Triple-Negative Breast Cancer (TNBC), which is the most aggressive and invasive form of the disease. This type of breast cancer has the worst prognosis, a decreased survival rate, and no targeted therapy. Over the decades, interest in pre- (Neoadjuvant) and post- (Adjuvant) chemotherapy treatments, in the management of TNBC has increased. Therefore, we evaluated the Adjuvant and Neoadjuvant effects of anti-telomerases (BIBR 1532 and GV6) with anthracycline-based …

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Climate, Environmental And Socio-Economic Change: Weighing Up The Balance In Vector-Borne Disease Transmission, Paul E. Parham, Joanna Waldock, George K. Christophides, Deborah Hemming, Folashade Agusto, Katherine J. Evans, Nina Fefferman, Holly Gaff, Abba Gumel, Shannon Ladeau Jan 2015

Climate, Environmental And Socio-Economic Change: Weighing Up The Balance In Vector-Borne Disease Transmission, Paul E. Parham, Joanna Waldock, George K. Christophides, Deborah Hemming, Folashade Agusto, Katherine J. Evans, Nina Fefferman, Holly Gaff, Abba Gumel, Shannon Ladeau

Biological Sciences Faculty Publications

Arguably one of the most important effects of climate change is the potential impact on human health. While this is likely to take many forms, the implications for future transmission of vector-borne diseases (VBDs), given their ongoing contribution to global disease burden, are both extremely important and highly uncertain. In part, this is owing not only to data limitations and methodological challenges when integrating climate-driven VBD models and climate change projections, but also, perhaps most crucially, to the multitude of epidemiological, ecological and socio-economic factors that drive VBD transmission, and this complexity has generated considerable debate over the past 10-15 …

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Effects Of Tgf-Β1 And Il-33 On Mast Cell Function, Victor S. Ndaw Jan 2015

Effects Of Tgf-Β1 And Il-33 On Mast Cell Function, Victor S. Ndaw

Theses and Dissertations

TGFβ is involved in many pathological conditions, including autoimmune disorders, cancer, and cardiovascular and allergic diseases. We have previously found that TGFβ can suppress IgE-mediated mast cell activation in human and mouse mast cells in vitro. IL-33 is a recently discovered member of the IL-1 family capable of inducing mast cell responses and enhancing IgE-mediated activation. In this study, we investigated the effects of TGFβ on IL-33-mediated mast cell activation. Bone marrow-derived mast cells cultured in TGFβ -1, -2, or -3 showed reduced IL-33-mediated production of TNF, IL-6, IL-13 and MCP-1, in a concentration-dependent manner. Furthermore, TGFβ also reduced …

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Behavioral Response Of Pacific Lamprey (Entosphenus Tridentatus) To Predator Odors, Laurie L. Porter Jan 2015

Behavioral Response Of Pacific Lamprey (Entosphenus Tridentatus) To Predator Odors, Laurie L. Porter

All Master's Theses

Pacific lamprey (Entosphenus tridentatus), a species facing serious threats to their existence, experience a number of challenges in reaching their desired spawning grounds during the adult migratory phase, and predators are suspected to be one of these challenges. Understanding if Pacific lamprey respond to predator odorants may provide a management tool for use in conjunction with attractants in guiding lamprey to suitable spawning habitat and deterring them from poor habitat. Previous research has failed to explore Pacific lamprey response to predator odorants, although much research exists on attractant odorants. In our study, we tested Pacific lamprey response to …

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Density Dependent Effects On Cutthroat And Brook Trout Behavioral Competition And Energy Expenditure, Ethan Daniel Green Jan 2015

Density Dependent Effects On Cutthroat And Brook Trout Behavioral Competition And Energy Expenditure, Ethan Daniel Green

All Master's Theses

Cutthroat Trout (Oncorhynchus clarki) are being displaced rangewide by invasive Brook Trout (Salvelinusfontinalis). There are many potential causes of displacement but the underlying mechanisms of the invasion and displacement are not well understood. Increased population densities in streams in which Cutthroat Trout and Brook Trout occur in sympatry appears to have detrimental effects on Cutthroat Trout. In this study, I compared the competitive behaviors and activity allocation patterns of Brook Trout and Cutthroat Trout at varying population densities in a simulated stream aquarium. I concluded that Brook Trout likely have a behavioral advantage over Cutthroat Trout …

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United In Diversity: Mechanosensitive Ion Channels In Plants, Eric S. Hamilton, Angela M. Schlegel, Elizabeth S. Haswell Jan 2015

United In Diversity: Mechanosensitive Ion Channels In Plants, Eric S. Hamilton, Angela M. Schlegel, Elizabeth S. Haswell

Biology Faculty Research

Mechanosensitive (MS) ion channels are a common mechanism for perceiving and responding to mechanical force. This class of mechanoreceptors is capable of transducing membrane tension directly into ion flux. In plant systems, MSion channels have been proposed to play a wide array of roles, from the perception of touch and gravity to the osmotic homeostasis of intracellular organelles. Three families of plant MS ion channels have been identified: the MscS-like (MSL), Mid1-complementing activity (MCA), and two-pore potassium (TPK) families. Channels from these families vary widely in structure and function, localize to multiple cellular compartments, and conduct chloride, calcium, and/or potassium …

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A Network For Integrating Bioinformatics Into Life Sciences Education, Sarah C.R. Elgin, Elizabeth Dinsdale, Neal Grandgenett, William Morgan, Anne Rosenwald, William Tapprich, Eric W. Triplett, Mark A. Pauley Jan 2015

A Network For Integrating Bioinformatics Into Life Sciences Education, Sarah C.R. Elgin, Elizabeth Dinsdale, Neal Grandgenett, William Morgan, Anne Rosenwald, William Tapprich, Eric W. Triplett, Mark A. Pauley

Biology Faculty Research

No abstract provided.

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Biology: Inquiry-Based Learning Assignment, Richa Gupta Jan 2015

Biology: Inquiry-Based Learning Assignment, Richa Gupta

Open Educational Resources

Students perform a literature review on a biology topic, choose 2-3 papers published in scientific journals to discuss in their assignment, provide a final opinion on the topic, and some voluntarily present on their selected topic.

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Introduction To Neuroscience, Charles Keller Jan 2015

Introduction To Neuroscience, Charles Keller

Open Educational Resources

Working with a partner from the class, students will choose a topic of interest relevant to the field of neuroscience, explore the relevant literature, write a 5-7 page paper on the topic, and present the results of their research to the class in the form of a 5-10 minute oral presentation.

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Human Anatomy And Physiology I (Laboratory), Cecilia Ortiz Jan 2015

Human Anatomy And Physiology I (Laboratory), Cecilia Ortiz

Open Educational Resources

Students will be able to understand and do the following: describe the mechanism by which osmosis and different types of diffusion occur and compare and contrast the effects of hypertonic, isotonic and hypotonic conditions on cells.

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Effective Use Of Trails For Assessing Terrestrial Salamander Abundance And Detection: A Case Study At Great Smoky Mountains National Park, Joseph Milanovich, Daniel J. Hocking, William E. Peterman, John A. Crawford Jan 2015

Effective Use Of Trails For Assessing Terrestrial Salamander Abundance And Detection: A Case Study At Great Smoky Mountains National Park, Joseph Milanovich, Daniel J. Hocking, William E. Peterman, John A. Crawford

Biology: Faculty Publications and Other Works

The conservation and management of wildlife species is contingent on estimating distribution and abundance. Sampling of wildlife requires repeated visits to accurately determine species occurrence and to quantify abundance across temporal and spatial scales. The use of trails to sample wildlife populations is increasing and offers opportunities to potentially sample more frequently, with increased ease of access, and less disturbance to habitats, which can be important in sensitive natural areas. We examined capture dataof terrestrial salamanders within Great Smoky Mountains National Park to determine if detection and abundance estimates from trail and non-trail transects were significantly different. Across two, 3-week …

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Assessing The Contributions Of Two Stocked Strains On A Minnesota-Wisconsin Border Water, Douglas Zentner Jan 2015

Assessing The Contributions Of Two Stocked Strains On A Minnesota-Wisconsin Border Water, Douglas Zentner

Journal of Earth and Life Science

Stocking has long been used as a management tool in the United States even though many of its effects remain cryptic. Past comparisons between stocked strains were generally based on the survival and growth rates of physically marked individuals. More recently the use of genetic markers to assess contributions of stocked individuals has been employed by fisheries managers. Using 13 microsatellite markers we assessed the contributions of two muskellunge (Esox masquinongy) strains (Wisconsin and Leech) stocked into the St. Louis River Estuary. Ancestry in the adult samples remained relatively constant with differences only observed between the 2008 and …

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Using Wild I.D. As A Reliable Source For Mark And Recapture Studies On Northern Pike (Esox Lucius), Martin Evans Jan 2015

Using Wild I.D. As A Reliable Source For Mark And Recapture Studies On Northern Pike (Esox Lucius), Martin Evans

Journal of Earth and Life Science

Mark and recapture studies are a very popular method fisheries biologists use to assess certain fish populations in lakes. This process can be very labor intensive and expensive. Wild I.D. is free software developed by Dartmouth College that uses SIFT program to find unique features in photographs. Initially developed for identification of African land mammals the program gives each photo a score and percent match to other photos. Northern pike were used in this study to determine if the program can recognize simulated recapture events. Photos of sample fish were taken at two separate locations, the photos were then copied …

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Juvenile Chinook Salmon Diet In Comparison To Invertebrate Drift In The Merced River, California, Tyler Orgon Jan 2015

Juvenile Chinook Salmon Diet In Comparison To Invertebrate Drift In The Merced River, California, Tyler Orgon

Journal of Earth and Life Science

Diets and habitat types of salmonids have been widely studied in disturbed streams and rivers. The Merced River in California has a long history of flooding, damming, levee failures, and channel reconstruction events that has disrupted the aquatic ecosystem. This 30 day study was conducted in the Robinson Reach of the Merced River using juvenile Chinook salmon (Oncorhynchus tshawtscha). The objectives were to try and understand if juvenile Chinook salmon were selecting certain invertebrate species, determine if there were any differences in diets between habitat types, and determine whether diets and drift samples were different. Diets consisted of …

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Effects Of Cooler Northern Waters On Growth Rates In Bluegill And Pumpkinseed Sunfish, Alec Spurbeck Jan 2015

Effects Of Cooler Northern Waters On Growth Rates In Bluegill And Pumpkinseed Sunfish, Alec Spurbeck

Journal of Earth and Life Science

The Centrarchidae family range spans all across the United States making them a favorite target for many recreational anglers. In northern Minnesota, most water bodies do not reach optimal growth temperatures for the sunfishes, putting them right on the edge of their suitable range and potentially slowing overall growth. Therefore, the main objectives of this study were to 1) determine if sunfish growth rates respond to the severity of winter and 2) investigate growth differences between two sunfish species. Growth rates of bluegill (Lepomis macrochirus) and pumpkinseed (Lepomis gibbosus) were estimated from Big Sugar Bush Lake, …

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Spatial And Temporal Factors Influencing Northern Pike Fry Density Estimates, Nate Stott Jan 2015

Spatial And Temporal Factors Influencing Northern Pike Fry Density Estimates, Nate Stott

Journal of Earth and Life Science

The Tamarac River in northern Minnesota is an important spawning site for northern pike (Esox lucius) in the Red Lake system. However, little is known about fry production in the river. This study investigates migration densities and spatial and temporal factors influencing northern pike fry density estimates. Northern pike fry were collected with drift nets between 28 May and 4 June 2014. Thirty northern pike fry were randomly selected to have total length measured from each sample. Northern pike fry were observed in the first sample day. Northern pike fry density maxima occurred on 29 May at midnight …

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A Genetic Based Approach To Management And Stocking Of Muskellunge, Douglas Zentner Jan 2015

A Genetic Based Approach To Management And Stocking Of Muskellunge, Douglas Zentner

Journal of Earth and Life Science

Fish stocking has long been used as a management tool among fisheries biologists and continues to be met with popular public opinion. Despite perceived simplicity, the full effects of stocking remain cryptic for many fish populations. Stocking has frequently been used to maintain or establish successful muskellunge (Esox masquinongy) populations. Coupled with other management practices the Minnesota Department of Natural Resources (MNDNR) has established Minnesota as a premier muskellunge fishery. From 1958 to 2012 the MNDNR stocked three strains of muskellunge in Big Mantrap Lake, MN. The first from Shoepack Lake, MN (hereafter Shoepack-strain) was used until the …

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Human Anatomy And Physiology Ii, Jhonny Ordonez Jan 2015

Human Anatomy And Physiology Ii, Jhonny Ordonez

Open Educational Resources

Students will learn and understand the importance of reflexes in human body, understand how the nerve impulse travels through the nerves and how to test a reflex.

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Effect Of High Light Intensity On Photoinhibition, Oxyradicals And Artemisinin Content In Artemisia Annua L., Mary E. Poulson, T. Thai Jan 2015

Effect Of High Light Intensity On Photoinhibition, Oxyradicals And Artemisinin Content In Artemisia Annua L., Mary E. Poulson, T. Thai

Biology Faculty Scholarship

Artemisia annua L. produces a compound called artemisinin that is a potent anti-malarial compound. However concentration of artemisinin within the plant is typically low (less than 0.8% of dry mass) and currently supply of the drug by the plant does not meet world demand. This investigation was carried out to determine whether high intensity light treatment would increase production of artemisinin in leaves of A. annua. Photoinhibition (14%) was induced in leaves of A. annua when they were subjected to 6 h of high-intensity light [2,000 μmol (photon) m–2 s–1]. Maximum photochemical efficiency of PSII showed …

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Protemic Identification Of Germline Proteins In Caenorhabditis Elegans, B Elizabeth Turner, Sophia M. Basecke, Grace E. Bazan, Eric S. Dodge, Cassy M. Haire, Dylan J. Heussman, Chelsey L. Johnson, Chelsea K. Mukai, Adrianna M. Naccarati, Sunny-June Norton, Jennifer R. Sato, Chihara O. Talavera, Michael V. Wade, Kenneth J. Hillers Jan 2015

Protemic Identification Of Germline Proteins In Caenorhabditis Elegans, B Elizabeth Turner, Sophia M. Basecke, Grace E. Bazan, Eric S. Dodge, Cassy M. Haire, Dylan J. Heussman, Chelsey L. Johnson, Chelsea K. Mukai, Adrianna M. Naccarati, Sunny-June Norton, Jennifer R. Sato, Chihara O. Talavera, Michael V. Wade, Kenneth J. Hillers

Biological Sciences

Sexual reproduction involves fusion of 2 haploid gametes to form diploid offspring with genetic contributions from both parents. Gamete formation represents a unique developmental program involving the action of numerous germline-specific proteins. In an attempt to identify novel proteins involved in reproduction and embryonic development, we have carried out a proteomic characterization of the process in Caenorhabditis elegans. To identify candidate proteins, we used 2D gel electrophoresis (2DGE) to compare protein abundance in nucleus-enriched extracts from wild-type C. elegans, and in extracts from mutant worms with greatly reduced gonads (glp-4(bn2) worms reared at 25°C); 84 proteins whose …

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Spatial Variation Of Bacterial Communities On The Leaves Of Southern Magnolia Trees, Heather L. Valdin Jan 2015

Spatial Variation Of Bacterial Communities On The Leaves Of Southern Magnolia Trees, Heather L. Valdin

Honors Theses

Bacteria are found in almost all environments on earth, including on the surface of other living things. Bacteria that exist on the leaves of plants (the phyllosphere) are of interest because of the potentially harsh nature of the environment they inhabit, and of applied interest. Although there has been a substantial amount of research in phyllosphere microbiology, few studies have examined the effect of spatial variation on phyllosphere community composition. This project investigated the effect of spatial variation on the bacterial communities living on the surface of Magnolia grandiflora leaves. Following DNA extraction, next generation sequencing of 16S rRNA gene …

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Differences In Bacterial Communities On Decaying Leaf Litter Of Different Tress In Response To Burning As A Forest Restoration Technique, Allison Marcum Jan 2015

Differences In Bacterial Communities On Decaying Leaf Litter Of Different Tress In Response To Burning As A Forest Restoration Technique, Allison Marcum

Honors Theses

Decomposition is the process by which organic matter gets degraded into basic components to provide energy for decomposer microorganisms and to also make nutrients available for plant uptake. Leaf litter decomposition is an important process and influences the nutrient cycling and the productivity and structure of the entire ecosystem; however, few studies have examined the bacterial communities on decomposing litter, especially how they may vary between tree species or in woodlands subject to ecological restoration. Such restoration has become important, as fire suppression beginning in the 1920s has impacted the structure of forest ecosystems through the process of mesophication, diminishing …

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Feasibility Of Incorporating Electrogastrography Into An Undergraduate Physiology Laboratory Curriculum, Lauren A. Foropoulos Jan 2015

Feasibility Of Incorporating Electrogastrography Into An Undergraduate Physiology Laboratory Curriculum, Lauren A. Foropoulos

Honors Theses

This study aimed to detect the effects of mental stress on gastric motility in college students through the use of cutaneous electrogastrography (EGG). Additionally, the study aimed to determine the feasibility of creating a laboratory curriculum for a cutaneous electrogastrography experiment that an undergraduate could perform, analyze, and interpret during a three hour physiology lab. Through this study and the laboratory procedures designed from the experiment, undergraduates will be able to interpret the physiological aspects of gastrointestinal function within the human body, including how it is effected by mental stress. According to previous studies by Yin et al. (2004) and …

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An Examination Of Microbial Populations In Different Brands And Flavors Of Ice Cream, Madeline Campbell Jan 2015

An Examination Of Microbial Populations In Different Brands And Flavors Of Ice Cream, Madeline Campbell

Honors Theses

Outbreaks of foodborne illness are of concern to many Americans. While pasteurization of ingredients and freezing eliminates most microbiological hazards, ice cream can act as a vehicle for pathogen transmission and be a cause of foodborne disease. This study examined the bacterial content of seven nationally distributed brands of ice cream and one local farmer's market brand. The aims of the study were to (1) determine if there was a higher bacterial content in chocolate and strawberry ice cream compared to vanilla ice cream, potentially because of more ingredients and more opportunities for bacterial contamination in those flavors, and (2) …

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