Biology Commons^™

Isolation And Characterization Of Galactinol Synthases From Hybrid Poplar, Faride Unda, Thomas Canam, Lindsay Preston, Shawn D. Mansfield

Faculty Research & Creative Activity

The raffinose family of oligosaccharides (RFOs) serve as transport carbohydrates in the phloem, storage compounds in sink tissues, and putative biological agents to combat both abiotic and biotic stress in several plant species. To investigate further the functional roles of this class of compounds in trees, two cDNAs encoding galactinol synthase (GolS, EC 2.4.1.123), which catalyses the first step in the biosynthesis of RFOs, were identified and cloned from hybrid poplar (Populus alba3grandidentata). Phylogenetic analyses of the Populus GolS isoforms with other known GolS proteins suggested a putative role for these enzymes during biotic or abiotic stress in hybrid poplar. …

Effectiveness And Utility Of Acoustic Recordings For Surveying Tropical Birds, Antonio Celis-Murillo, Jill L. Deppe, Michael P. Ward

Faculty Research & Creative Activity

Although acoustic recordings have recently gained popularity as an alternative to point counts for surveying birds, little is known about the relative performance of the two methods for detecting tropical bird species across multiple vegetation types. During June and July 2008, we collected species detection/nondetection data to compare the performance of a quadraphonic acoustic recording system and point counts for estimating species richness and composition and detection probabilities of 15 rare, moderately common, and common tropical bird species across six structurally distinct vegetation types (coastal dune scrub, mangrove, low-stature deciduous thorn forest, early and late successional medium-stature semievergreen forest, and …

The Swimming Behavior Of The Aquatic Larva Of Neoneuromus Ignobilis (Megaloptera: Corydalidae: Corydalinae)., Cheng-Quan Cao, Zhiwei Liu, Shen-Zhi Chen, Chao Tong

Faculty Research & Creative Activity

In order to explore the pattern and significance of swimming, through photos and videos we observed and recorded the swimming behavior of the aquatic larvae of Megaloptera in detail for the first time using the endemic Chinese species Neoneuromus ignobilis Navas, 1932 as the test insect, which were collected from the Dadu River and reared in nature-simulated environments. Four swimming postures are recognized and described herein in detail, i. e., vertical, parallel, back and side swimming, and these postures were used by the larvae disproportionately, with a frequency of 89.08%, 5. 49%, 4. 40% and 0. 61% , respectively. The …

The Swimming Behavior Of The Aquatic Larva Of Neoneuromus Ignobilis (Megaloptera: Corydalidae: Corydalinae)., Cheng-Quan Cao, Zhiwei Liu, Shen-Zhi Chen, Chao Tong

Faculty Research & Creative Activity

In order to explore the pattern and significance of swimming, through photos and videos we observed and recorded the swimming behavior of the aquatic larvae of Megaloptera in detail for the first time using the endemic Chinese species Neoneuromus ignobilis Navas, 1932 as the test insect, which were collected from the Dadu River and reared in nature-simulated environments. Four swimming postures are recognized and described herein in detail, i. e., vertical, parallel, back and side swimming, and these postures were used by the larvae disproportionately, with a frequency of 89.08%, 5. 49%, 4. 40% and 0. 61% , respectively. The …

Mite Dna In Mantle Clips, Robert A. Krebs, Brian D. Allen, Na'tasha M. Evans

McNair Scholars Works

No abstract provided.

It Isn't Always Sexy When Both Are Bright And Shiny: Considering Alternatives To Sexual Selection In Elaborate Monomorphic Species, K. A. Tarvin, Troy G. Murphy

Biology Faculty Research

Since the dawn of abstract thinking, humans have wondered about the seemingly unnecessary elaborate ornamentations of birds. Gaudy colours, cumbersome tails, complex vocalizations and bizarre displays are found in bird species from all corners of the globe. Darwin (1871) provided an elegant explanation for the existence of these non-utilitarian traits: they increase mating success, and although they may impair survival, the costs of producing and bearing elaborate ornaments can be repaid in the currency of additional offspring. Darwin’s model still serves as the foundation for our concept of sexual selection but great strides have been made in our understanding of …

Carotenoid-Based Ornaments Of Female And Male American Goldfinches (Spinus Tristis) Show Sex-Specific Correlations With Immune Function And Metabolic Rate, R. J. Kelly, Troy G. Murphy, K. A. Tarvin, G. Burness

Biology Faculty Research

Conspicuous ornamentation has been linked to immunological and physiological condition in males of many species. In species where both sexes are ornamented, it is unclear whether the signal content of ornaments differs between males and females. We examined the immunological and physiological correlates of carotenoid-based bill and plumage ornamentation in American goldfinches Spinus tristis, a species in which bright orange bills are sexually monomorphic but yellow plumage is sexually dimorphic during the breeding season. Because bill color is dynamic over short periods while plumage color is static over longer time frames, we tested whether these signals have the potential to …

Prey Responses To Predator Chemical Cues: Disentangling The Importance Of The Number And Biomass Of Prey Consumed, Michael W. Mccoy, Justin C. Touchon, Tobias Landberg, Karen M. Warkentin, James R. Vonesh

Biology Publications

To effectively balance investment in predator defenses versus other traits, organisms must accurately assess predation risk. Chemical cues caused by predation events are indicators of risk for prey in a wide variety of systems, but the relationship between how prey perceive risk in relation to the amount of prey consumed by predators is poorly understood. While per capita predation rate is often used as the metric of relative risk, studies aimed at quantifying predator-induced defenses commonly control biomass of prey consumed as the metric of risk. However, biomass consumed can change by altering either the number or size of prey …

Dopamine-Beta-Hydroxylase And Norepinephrine Transporter Immunoreactivity In Rat Taste Buds, Jenna Ashley Walton

Electronic Theses and Dissertations

Of the handful of neurotransmitters modulating taste response, norepinephrine (NE) may be the least understood. Although the physiological role of NE has been tested, it is still unclear whether the rat taste bud may serve as an endogenous source of neurotransmitter or if it must be taken up from outside the taste bud. Immunocytochemical analysis of DBH has shown that DBH–LIR is present in taste cells of the rat circumvallate papillae. Specifically, DBH–LIR is present in a subset of Type II taste cells. Nearly all (98%) DBH–LIR cells express PLCβ2–LIR, and about half (41%) of PLCβ2–LIR cells express DBH–LIR. DBH–LIR …

Estradiol Modulation Of Calcium Dynamics In Pituitary Mmq Lactotroph Cells, Monika Pauckova

Electronic Theses and Dissertations

Pituitary lactotrophs are excitable cells that exhibit spontaneous, calcium influx triggering prolactin (PRL) secretion to stimulate lactation. Lactotrophs express estrogen receptors (ER) and are a well established estrogen-responsive cell system. 17B-estradiol (E2) is known to directly affect lactotrophs by increasing PRL transcription and biosynthesis, intracellular Ca2+ concentration ([Ca2+]i) and PRL secretion. This study demonstrates that the MMQ clonal cell line, isolated from the 7315a rat pituitary tumor, is a model lactotroph cell line that is E2-responsive. Spontaneous and evoked Ca2+ transients were especially sensitive to L-type channel block, but not affected by block of omega-conotoxin-GVIA-sensitive CaV channels or TTX-sensitive voltage-gated …

Pomc Is Expressed In Pancreatic Alpha Cells, Samia M.M Farrara

Electronic Theses and Dissertations

Pancreatic alpha cells secrete glucagon to increase blood glucose during hypoglycemia. Currently, the mechanisms that initiate glucagon secretion are not well understood. This study investigates the expression of pro-opiomelanocortin (POMC), a potent regulator of glucagon secretion, in alpha cells.

Insulin tolerance tests in mice lacking the POMC gene showed a correlation between alpha MSH, and glucagon secretion from alpha cells (Hochgeschwender et al. 2003). Results from a previous study showed that the POMC product, melanocortin á-MSH, acts through a melanocortin receptor 4 (MC4R) at the surface of the alpha cells to trigger the release of glucagon [Angleson, unpublished data, Lumsden …

Investigation Of The Importance Of Rock Chemistry For Saxicolous Lichen Communities Of The New Idria Serpentinite Mass, San Benito County, California, Usa, N Rajakaruna, K Knudsen, A Fryday, R E. O’Del, N Pope, F C. Olday, S Woolhouse

Faculty Publications, Biological Sciences

Although several lichen inventories exist for European ultramafic sites, only four surveys of serpentine lichens for North America have been published to date. Of those, only one has been conducted in California. We conducted a survey of saxicolous lichens from ultramafic rocks (including nephrite, partially serpentinized peridotite, and serpentinite) and non-ultramafic rocks (including silica-carbonate, shale, and sandstone) at the New Idria serpentinite mass, San Benito County, California. X-ray Fluorescence Analysis of the rocks from which the lichens were collected revealed significant elemental differences between the ultramafic and non-ultramafic rocks for 26 of the 32 major and trace elements analyzed. We …

The Discovery Of Insulin: A Case Study Of Scientific Methodology, William D. Stansfield

Biological Sciences

The nature of scientific research sometimes involves a trial-and-error procedure. Popular reviews of successful results from this approach often sanitize the story by omitting unsuccessful trials, thus painting the rosy impression that research simply follows a direct route from hypothesis to experiment to scientific discovery. The discovery of insulin is a classical case study in this genre that begs for an explanation to our students because it is so often ignored or misrepresented even in biology and physiology textbooks.

Bioluminescence In The High Arctic During The Polar Night, J. Berge, A.S. Båtnes, G. Johnsen, S.M. Blackwell, Mark A. Moline

Biological Sciences

This study examines the composition and activity of the planktonic community during the polar night in the high Arctic Kongsfjord, Svalbard. Our results are the first published evidence of bioluminescence among zooplankton during the Arctic polar night. The observations were collected by a bathyphotometer detecting bioluminescence, integrated into an autonomous underwater vehicle, to determine the concentration and intensity of bioluminescent flashes as a function of time of day and depth. To further understand community dynamics and composition, plankton nets were used to collect organisms passing through the bathyphotometer along with traditional vertical net tows. Additionally, using a moored bathyphotometer closed …

Acute Alcohol Ingestion And Sympathetic Neural Responses During Orthostatic Stress In Humans, Sarah F. Stream

Dissertations, Master's Theses and Master's Reports - Open

Acute alcohol consumption has been reported to decrease mean arterial pressure (MAP) during orthostatic challenge, a response that may contribute to alcohol-mediated hypotension and eventually syncope. Muscle sympathetic nerve activity (MSNA) increases during orthostatic stress to help maintain MAP, yet the influence of alcohol on MSNA during orthostatic stress has not been determined. We hypothesized that alcohol ingestion would blunt arterial blood pressure and MSNA responses to progressive lower body negative pressure (LBNP). MAP, MSNA, and heart rate (HR) were recorded during progressive LBNP (-5, -10, -15, -20, -30, and -40 mmHg; 3 min/stage) in 30 subjects(age 24 ± 1 …

Functional Analysis Of Rice Bidirectional Promoters, Surendar R. Dhadi

Dissertations, Master's Theses and Master's Reports - Open

Bidirectional promoters regulate adjacent genes organized in a divergent fashion (head to head orientation). Several Reports pertaining to bidirectional promoters on a genomic scale exists in mammals. This work provides the essential background on theoretical and experimental work to carry out a genomic scale analysis of bidirectional promoters in plants.

A computational study was performed to identify putative bidirectional promoters and the over-represented cis-regulatory motifs from three sequenced plant genomes: rice (Oryza sativa), Arabidopsis thaliana, and Populus trichocarpa using the Plant Cis-acting Regulatory DNA Elements (PLACE) and PLANT CARE databases. Over-represented motifs along with their possible …

Uptake And Fate Of Hexahydro-1,3,5-Trinitro-1,3,5-Triazine By Chrysopogon Zizanioides, Claire M. Doskey

Dissertations, Master's Theses and Master's Reports - Open

Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is a nitramine compound that has been used heavily by the military as an explosive. Manufacturing, use, and disposal of RDX have led to several contamination sites across the United States. RDX is both persistent in the environment and a threat to human health, making its remediation vital. The use of plants to extract RDX from the soil and metabolize it once it is in the plant tissue, is being considered as a possible solution. In the present study, the tropical grass Chrysopogon zizanioides was grown hydroponically in the presence RDX at 3 different concentration levels: 0.3, 1.1, …

Current Methods For Detecting The Presence Of Botulinum Neurotoxins In Food And Other Biological Samples, Luisa W. Cheng, Kirkwood M. Land, L. H. Stanker

College of the Pacific Faculty Books and Book Chapters

No abstract provided.

Antimicrobial And Antioxidant Properties Of Artemisia L. Species From Western Anatolia, Şüra Baykan Erel, Gottfried Reznicek, Serdar Gökhan Şenol, Nefi̇se Ülkü Karabay Yavaşoğlu, Si̇bel Konyalioğlu, Ahmet Ulvi̇ Zeybek

Turkish Journal of Biology

Artemisia L. taxa (A. absinthium L., A. arborescens L., A. campestris L., A. scoparia Waldst. & Kit., A. santonicum L., and A. vulgaris L.) naturally distributed through western and southwestern Turkey were chosen as experimental materials in this study. Essential oils of the aerial parts of these Artemisia species were isolated by hydrodistillation and analyzed by gas chromatography-mass spectrometry. The major components were identified as sabinene in A. absinthium (17.56%), camphor in A. arborescens (33.39%), 1,2-dehydro acenaphthylene in A. campestris and A. scoparia (20.71% and 11.80%, respectively), and \alpha-thujone in A. vulgaris and A. santonicum (56.13% and 39.46%, respectively). Essential …

Changes In Seed Reserve Composition During Germination And Initial Seedling Development Of Safflower (Carthamus Tinctorius L.), Muhammet Tonguç, Ruzi̇ye Elkoyunu, Sabri̇ Erbaş, Yaşar Karakurt

Turkish Journal of Biology

The present study was carried out to determine the changes in some reserve molecules of safflower seeds during germination and early seedling growth. The seeds of 2 safflower cultivars were used as plant material (Dinçer 5-118 and Montola 2000). Seeds were germinated for 72 h and observations were taken every 12 h throughout the study. Changes in total oil content (%), total protein content (%), total soluble sugar contents (TSS, mg g^{-1}), and reducing sugar contents (RS, mg g^{-1}) were determined for each cultivar during germination and seedling growth. The oil content of the cultivars decreased from 54.7% and 57.4% …

Respiratory Evaporative Water Loss During Hovering And Forward Flight In Hummingbirds, Donald R. Powers, Philip W. Getsinger, Bret W. Tobalske, Susan M. Wethington, Sean D. Powers, Douglas R. Warrick

Faculty Publications - Department of Biological & Molecular Science

Hummingbirds represent an end point for small body size and water flux in vertebrates. We explored the role evaporative water loss (EWL) plays in management of their large water pool and its use in dissipating metabolic heat. We measured respiratory evaporative water loss (REWL) in hovering hummingbirds in the field (6 species) and over a range of speeds in a wind tunnel (1 species) using an open-circuit mask respirometry system. Hovering REWL during the active period was positively correlated with operative temperature (Te) likely due to some combination of an increase in the vapor-pressure deficit, increase in lung ventilation rate, …

Effects Of Angiotensin Ii On Mitochondrial Physiology In Normal (Pwr-1e) And Malignant (Lncap) Prostate Epithelium Cell Lines, Jeff Duerr, Jennifer R. Kordosky

Faculty Publications - Department of Biological & Molecular Science

Prostate cancer is diagnosed in over 186,000 men in the United States and accounts for more than 28,000 deaths annually. The epidemiology of prostate cancer is identified by risk factors including genetics, diet, smoking alcohol consumption, chronic inflammation, hypertension and age. Angiotensen II (AngII) is a major effector peptide of the rennin-angiotensin system (RAS), and is an important factor in hypertension. RAS interacts with the autocrine/paracrine system, and relates to cell growth and differentiation in some organs. Investigators have reported that exogenous application of Ang II to LNCaP cells results in the activation of Akt signaling, increased activity of SOD, …

Reproductive Strategy Of Male And Female Eastern Spiny Lizards Sceloporus Spinosus (Squamata: Phrynosomatidae) From A Region Of The Chihuahuan Desert, Mexico, A. Ramirez-Bautista, B. P. Stephenson, X. Hernandez-Ibarra, U. Hernandez-Salinas, R. Cruz-Elizalde, A. Lozano, Geoffrey R. Smith

Faculty Publications

No abstract provided.

The Molecular And Behavioral Characterization Of Cav1.3 Over-Expressing Mice, Jamie Nicole Slater

Master's Theses and Doctoral Dissertations

Age-related cognitive decline refers to the memory impairment and difficulty learning new tasks that occurs during the normal process of aging. There are many possible changes that occur at the neuronal level that could account for age-related cognitive decline. One hypothesis suggests that a dysregulation of neuronal intracellular calcium concentration contributes to age-related cognitive deficits. Previous research suggests that an increase in neuronal L-type voltage-gated calcium channels (LVGCCs) occurs in the brain during aging. This increase could account for altered intracellular calcium concentration and lead to agerelated cognitive decline. A line of transgenic mice that over-express the LVGCC Ca_{V …}

A Contribution To The Knowledge Of The Ochid Bes Of The Los Amigos Biological Station, Peru (Hymenoptera, Apidae, Euglossini) [Abstract], R. Niemack, Ismael A. Hinojosa-Diaz, Daniel J. Bennett, C. S. Chaboo

Faculty Publications

No abstract provided.

Effects Of An Invasive Plant, Chinese Tallow (Triadica Sebifera), On Development And Survival Of Anuran Larvae, Taylor B. Cotten, Matthew Kwiatkowski, Daniel Saenz, Michael Collyer

Faculty Publications

Amphibians are considered one of the most threatened vertebrate groups. Although numerous studies have addressed the many causes of amphibian population decline, little is known about effects of invasive plants. Chinese tallow (Triadica sebifera) is an exotic deciduous tree that has invaded the southeastern United States. Amphibian larvae in environments invaded by T. sebifera may be impacted negatively as autumn leaf litter decomposes in natal areas. We compared effects of leaf litter decomposition from T. sebifera and two native tree species on survival and development of four species of anuran larvae from eastern Texas. Larvae from Pseudacris fouquettei, Lithobates (Rana) …

New Species Of Macrocephalic Halictine Bees (Hymenoptera: Halictidae) [Abstract], Michael S. Engel, Ismael A. Hinojosa-Diaz, Daniel J. Bennett

Faculty Publications

No abstract provided.

Antimicrobial Activity Of Poplar Propolis On Mutans Streptococci And Caries Development In Rats, Soley Arslan, Si̇bel Si̇li̇ci̇, Duygu Perçi̇n, Ayşe Nedret Koç, Özgür Er

Turkish Journal of Biology

The influence of the different extracts of propolis and chlorhexidine on mutans streptococci and the development of dental caries in rats was examined in this study. Specifically, the antimicrobial effects of propolis extracts (including ethanol, methanol, chloroform, hexane, propylene glycol, and ethyl acetate) on Streptococcus mutans UA159 and Streptococcus sobrinus 6715 were studied. Minimal inhibitory concentration and minimal bactericidal concentration were determined using the National Committee for Clinical Laboratory Standards broth macrodilution method. In the animal study, the rats were infected with S. sobrinus and fed a cariogenic diet. They were treated topically twice a day with both ethanol extract …

Sequencing And Phylogenetic Analysis Of The Wsp Gene Of Wolbachia In Three Geographic Populations Of An Oak Gall Wasp, Andricus Mairei (Hymenoptera: Cynipidae), From Hunan, South China, Xiao-Hui Yang, Dao-Hong Zhu, Zhiwei Liu, Ling Zhao

Faculty Research & Creative Activity

To understand the effects of Wolbachia in Andricus mairei Kieffer , an oak gall wasp ( Cynipini, Cynipidae) that forms clusters of up to 30 monothalamous galls in the catkins of Quercus glandulifera var. brevipetiolata Nakai, we examined the presence and infection frequency of Wolbachia in three widely separated geographic populations of A. mairei in Hunan Province, South China, using polymerase chain reaction and sequence determination of the wsp gene of Wolbachia from the three populations. Our survey results showed that Wolbachia infection rates were unusually high in the three test populations : 100% for the male wasps from all …

Seed Biology Of Physaria Ludoviciana (Silvery Bladderpod; Brassicaceae ), An Endangered Species In Sand Prairies Of The Midwest, Marissa C. Grant, Ann E. Claerbout, Janice M. Coons, Henry R. Owen

Faculty Research & Creative Activity

Physaria ludoviciana (Nuttal) O'Kane & Al-Shehbaz (silvery bladderpod) is a state endangered species in Illinois, Minnesota and Wisconsin, the eastern edge of its range. Our goal was to investigate its seed biology (production, dispersal, seed bank longevity, afterripening, seed longevity after storage using germination and emergence, and seedling establishment). This information will be useful for conservation efforts of this endangered species. Over a ten-year period, various seed biology parameters were measured. Infructescences were counted to estimate seed production with 110–744 seeds per reproductive plant. Soil samples were collected at various distances to estimate dispersal where seeds dispersed less than a …