Biology Commons^™

Of Mice And Mozart: Testing Spatial Reasoning And Memory Of Rats, Kaitlin O'Toole

Honors Theses

An increase in spatial-temporal reasoning has been documented in the presence of Mozart's sonata K.448; this enhancement of physical and psychological activities is called the "Mozart Effect" (Hughes 2001). This effect has been recorded in humans and animals alike, and its influence reaches a myriad of applications including calming wild animals to increasing test scores in college students to lowering high blood pressure. Using a cheeseboard apparatus to test spatial reasoning in rats, this experiment recorded the results from maze tasks completed in the presence of sonata K.448 compared to trials completed in silence. Results showed a significant difference between …

Population Genetics Of The Potato Psyllid, Bactericera Cockerelli, Rebekah I. Chapman

Biology Theses

The potato psyllid (Bactericera cockerelli) is native to North America and occurs from Central America to Canada. A new disease of potatoes, Zebra Chip, has recently been associated with potato psyllid occurrence. Potato psyllids transmit a gram-negative α-proteobacter, Candidatus Liberibacter solanacearum, the putative causal agent of Zebra Chip in potatoes. Symptoms of Zebra Chip first appeared in potato plants grown outside of Saltillo, Mexico in 1994, and by 2000, this invasive disease complex was found in potatoes in south Texas. Since then, Zebra Chip has spread northward, throughout the central plains of the US and on the west coast. Potato …

Teaching Old Buildings New Tricks: Benefits Of Retrofitting Indianapolis Buildings With Green Roofs, Sarah Elizabeth Strobl

Undergraduate Honors Thesis Collection

Not your basic rooftop garden, green roofs contain growing media and are planted with various types of vegetation. Green roofs can be either intensive, with soil depths above 6 inches and increased size and attractiveness of vegetation, such as trees and shrubs, or extensive, with soil depths between 2-6 inches and low, moss or grass-like plants that require little care (Getter et al. 2009, Indianapolis DPW 2008, Obemdorfer et al. 2007, US DOE 2004).

Although green roofs are initially more expensive than traditional roofing techniques due to the cost of additional materials, the long-term cost savings are high and can …

Devil In A Hula Skirt: Comparative Conservation Efforts Between The United States And Australia Using Two Endemic Species As Case Studies, Sarah Catherine Kuchinsky

Undergraduate Honors Thesis Collection

There is a biodiversity crisis in our midst. An abundance of technology and innovation in the last 200 years has revamped our world but at the cost of pushing many species to extinction. Since the passing of the Endangered Species Act by Congress in 1973, more than 100 species in the United States have disappeared (Wilson 2006). The International Union for the Conservation of Nature (IUCN) stats that "19,265 species out of the 59,507 so far assessed as threatened with extinction," (IUCN 2011). Extinction is the result of a combination of factors caused by human environmental disturbance. Disturbances in the …

The Effect Of Photoperiod On Regulation Of Key Components Of The Life Cycle In The Bumble Bee Bombus Impatiens L. (Hymenoptera: Apidae)., Edgar Javier Hernandez

Dissertations

This study investigates how photoperiod affects internal characteristics of colonies of the temperate bumble bee Bombus impatiens particularly; development, reproduction, and social behavior. To answer this question I used an experimental approach using commercially obtained colonies of B. impatiens kept under controlled environmental conditions, and using the exposure to photoperiod as the only variable. Eight different photoperiod treatments were evaluated over the social phase of the colony’s life cycle. Colonies exposed to photoperiods that simulate the species natural temperate photoperiod exhibited larger growth rates, higher, oviposition rates, and higher brood survival than colonies exposed to constant photoperiods. Similarly, colonies exposed …

The Identification Of Arachnid Species In The Cockscomb Basin Wildlife Sanctuary, Lauren Auer

Honors Theses

Despite the known diversity of arachnids in neotropical ecozones there has been little considerable movement towards creating a collective means for identification of arachnids in Belize. Previous studies on ecotourism indicate positive trends between biodiversity education and conservation concern. This study was conducted to engage in a field-based study of arachnids in the Cockscomb Basin Wildlife Sanctuary to determine the most frequently encountered arachnid species. For this study, four separate locations were surveyed within the Sanctuary: three trail sites and the main camp area. Each site was surveyed during two alternate time periods to account for diurnal and nocturnal species. …

The Role Of Midline And Pexin10 In Regulating Drosophila Eye Development, John Forstall

Honors Theses

The Drosophila midline (mid) transcription factor gene encodes a highly conserved invertebrate ortholog of the mammalian Tbx20 gene essential for regulating the development of diverse tissues including the central nervous system (CNS), epidermis and heart. Decreasing mid transcript levels within larval eye discs using RNAi (mid- RNAi) results in poorly organized IPCs and in adult eyes, interommatidal bristles derived from sensory organ precursors are significantly reduced in number within the ventral eye field. We also observe gross abnormalities in bristle polarity, ommatidial organization, cellular adhesion and pigmentation in adult mid-RNAi flies. By combining a classical …

Determining The Diversity Of Wetland Fungi Through Molecular-Based Species Identification, Kimberly Chesteen

Honors Theses

This study used molecular techniques to examine the diversity of fungal species inhabiting leaf and stem litter of the freshwater emergent macrophyte, Typha angustifolia, during decomposition. Because morphological identification of fungal species is frequently inaccurate and underestimates the number of fungal species present, molecular techniques involving rRNA genes such as ITS regions (internal transcribed spacer regions) are used to better determine specific fungal species within a sample. In this study, samples of decaying leaf and stems litter of Typha angustifolia were obtained from Independence Lake, Michigan over the span of a year. DNA from litter samples obtained from the months …

Spiders Of Riparian Communities In The Cockscomb Basin Wildlife Sanctuary, Belize, Megan Chevis

Honors Theses

Currently there is little literature on what arachnids inhabit the tropics of Central America, especially within the country of Belize. This study aimed to find which spider species are found in riparian areas within the Cockscomb Basin Wildlife Sanctuary in central Belize and to distinguish trends among spiders and the microhabitats in which they live. Nocturnal samples were predicted to have greater diversity than diurnal samples at all sites. Pisauridae, Salticidae, and Lycosidae were predicted to be the most common spider families collected. Specimens were collected from three riparian sites located on trails within Cockscomb. Diurnal and nocturnal samples were …

The Effects Of An Informal Science Education Setting On Students’ Attitudes Towards Learning Science, Lauren Finley

Honors Theses

Informal science education is becoming increasingly popular in today’s classrooms, and for this reason, it is important to understand the effects of informal science education on students. In this study, the Children’s Attitude Survey (Wulf, Mayhew, & Finkelstein, 2009) was used to measure the effects of informal science education on students’ attitude toward science. Questionnaires were administered to two sample sets, and results indicated a slight decrease in attitude after participating in an informal science field trip. Continuing research in this area will provide more insight into why these effects were seen, and how informal science education can be best …

Host-Parasite Interactions In Galapagos Seabirds, Iris Ilena Levin

Dissertations

Parasites exhibit a wide range of life history strategies that contribute to different dispersal abilities, host specialization, transmission modes, life-cycle complexity and population structure. Understanding dispersal rates in hosts and parasites is instrumental in defining the scale at which coevolution may be occurring. In order to better understand how and when parasites move between different hosts, I studied a seabird – Hippoboscid fly ectoparasite (and vector) – Haemosporidian parasite system in the Galapagos Islands. I began by describing the Haemosporidian parasites of Galapagos seabirds, discovering a Plasmodium species parasite in Galapagos Penguins (Sphensicus mendiculus), and a new clade of Hippoboscid-vectored …

Evolution And Biogeography Of Fire-Eye Antbirds (Genus Pyriglena): Insights From Molecules And Songs, Marcos Maldonado Coelho

Dissertations

The importance of climatic and geologic factors as drivers of population differentiation and speciation in the Neotropical region has long been appreciated. However, many questions remain regarding their roles underlying the processes and patterns of diversification. Studies conducted in distinct regions containing a suite of geological and ecological conditions constitute ideal scenarios to assess the role of Pleistocene climatic changes, rivers, and mountain building as historical diversification mechanisms. In chapters 1 and 2, I used an integrative approach combining molecular phylogenetics, phylogeography and population genetics to elucidate the importance of climatic and geological factors as engines of diversification. I focused …

Salinity Preference Of Alaskan Threespine Stickleback: Test For Divergence In Halotaxis Between Ancestral And Landlocked Populations, David C. Fryxell

Honors Scholar Theses

Glacial retreat during the Pleistocene caused landlocking of anadromous Alaskan threespine stickleback, Gasterosteus aculeatus, furnishing a natural 'experiment' in osmoregulatory divergence. The objective of this study was to assess the effects of individual acclimation and population divergence on salinity preference. Full-sibling families of marine, anadromous, and freshwater-landlocked populations of stickleback were reared in common environments until 3 weeks post-hatch, then were split and acclimated to low or high salinity. At 6 to 8 weeks of age the six experimental groups were tested for salinity preference in a tank that offers fish a choice of compartments with different salinities arranged in …

Predictors Of 3pq And Quickboard Performance In Power-Oriented Collegiate Athletes, Brendan Putney

Honors Scholar Theses

The measuring of athletic performance via pre participation experiments has been the norm for a long time from high school sports, all the way to professional athletics. These tests help gauge an athlete's physical growth over the offseason, as well as aid in predicting their potential performance in the future. However, performance may diminish as multiple maximum effort assessments are completed due to fatigue. The purpose of this study was to examine targeted assessments for possible novel qualities as performance predictors. Baseline data was taken from a larger performance enhancement study for analysis. Twenty-six men (age: 25+4 yr; height 1.78+0.07m; …

Evolution Of The Bacteriorhodopsin Gene Bop In Haloarchaea, Maulik Jitesh Jani

Honors Scholar Theses

Rhodopsins are among the most studied protein families. They all incorporate a light based chromophore and an opsin. They are found in all three domains of life and can be found in diverse environments, such as the membrane of the haloarchaea, Haloarcula (Haa) marismortui, growing on salt flats in Death Valley, to within human tissues and deep sea water (Briggs and Spudich 2005). Rhodopsins can serve multifarious purposes, from phototaxis away from harmful light to efficient energy generation. Bacteriorhodopsin is the best studied rhodopsin in haloarchaea. The evolutionary pattern of bacteriorhodopsin through its gene, bop, is the main focus of …

The Role Of Actin Binding Proteins In Cell Motility, Elizabeth Ojukwu

Honors Scholar Theses

Actin binding proteins (ABPs) play key roles in the dynamic organization of the actin cytoskeleton during cell movement. The general localization of ABPs during dynamic cellular processes has been characterized. However, their specific functions and mechanisms of regulating the cytoskeleton during motile cellular processes remain unclear. Two approaches have been utilized to ask how these proteins contribute to cytoskeletal function during motility. Using homologous recombination, mutant cell lines lacking α-actinin, fimbrin or both proteins have been created. By analyzing mutants that lack these proteins, we hope to disrupt processes that require a functional cytoskeleton such as phagocytosis, random motility, development, …

Pathogen Response Genes Mediate Caenorhabditis Elegans Innate Immunity, Hannah L. Anthony

Celebration

Innate immunity is crucial in the response and defense against pathogens for invertebrates and vertebrates alike. The soil nematode Caenorhabditis elegans is a useful model to study the eukaryotic innate immune response to microbial pathogenesis. Prior research indicates that the protein receptor FSHR-1 plays an important role in the innate recognition of intestinal infection due to pathogen consumption. Determining what genes are controlled by FSHR-1 may uncover an unknown pathway that could increase not only the comprehension of the C. elegans immune system but also innate immunity generally. To characterize the function of FSHR-1, four candidate pathogen response genes that …

Remarkable Resilience Of Teeth (How Are Teeth So Brittle Yet So Resilient), Paul J. Constantino

Biological Sciences Faculty Research

Tooth enamel is inherently weak, with fracture toughness comparable with glass, yet it is remarkably resilient, surviving millions of functional contacts over a lifetime. We propose a microstructural mechanism of damage resistance, based on observations from ex situ loading of human and sea otter molars (teeth with strikingly similar structural features). Section views of the enamel implicate tufts, hypomineralized crack-like defects at the enamel–dentin junction, as primary fracture sources. We report a stabilization in the evolution of these defects, by ‘‘stress shielding’’ from neighbors, by inhibition of ensuing crack extension from prism interweaving (decussation), and by self-healing. These factors, coupled …

Macondo Crude Oil From The Deepwater Horizon Oil Spill Disrupts Specific Developmental Processes During Zebrafish Embryogenesis, T. Yvanka De Soysa, Allison Ulrich, Timo Friedrich, Danielle Pite, Shannon L. Compton, Deborah Ok, Rebecca L. Bernardos, Gerald B. Downes, Shizuka Hsieh, Rachael Stein, M. Caterina Lagdameo, Katherine Halvorsen, Lydia Rose Kesich, Michael J.F. Barresi

Biological Sciences: Faculty Publications

Background: The Deepwater Horizon disaster was the largest marine oil spill in history, and total vertical exposure of oil to the water column suggests it could impact an enormous diversity of ecosystems. The most vulnerable organisms are those encountering these pollutants during their early life stages. Water-soluble components of crude oil and specific polycyclic aromatic hydrocarbons have been shown to cause defects in cardiovascular and craniofacial development in a variety of teleost species, but the developmental origins of these defects have yet to be determined. We have adopted zebrafish, Danio rerio, as a model to test whether water accumulated fractions …

Food Web Subsidies In Intermittent Streams: Implications For Small Isolated Populations Of Native Brook Trout, Jennifer Lauren Courtwright

Masters Theses, 2010-2019

Recent studies in perennial streams have shown that reduction of stream-side vegetation can reduce terrestrial invertebrate inputs to streams and can cause trophic cascades throughout aquatic and terrestrial food webs; however, aquatic-terrestrial food web linkages have not been studied in intermittent streams. Food webs in intermittent streams may be even more dependent on terrestrial invertebrate fluxes because of limited aquatic invertebrate resources; thus intermittent streams may represent unique systems that warrant special attention. The objective of this research was first to quantify the abundance, biomass, and energetic content of available brook trout and insectivorous stream-side predator invertebrate resources in two …

Floristic Survey Of The Vascular Plants Of Shenandoah County, Virginia, Brinton Evan Domangue

Masters Theses, 2010-2019

The objective of this study was to create a comprehensive record of the vascular plants of Shenandoah County, Virginia. Specimens were collected from the field during the 2011 growing season (March 21 – October 27), and for part of the 2012 growing season (March 22 – May 30). Local herbaria of James Madison University (JMUH), Bridgewater College (BDWR), Eastern Mennonite University (HAVI) and Lord Fairfax Community College (LFCC) were also searched for pre-existing specimens. The results of the study were compared to records of the Digital Atlas of the Virginia Flora (2012), which currently maps the distribution of vascular plants …

Impacts Of Rdx Soil Contamination Across An Age Gradient For The Native Shrub Morella Cerifera., Stephen Via

Theses and Dissertations

Understanding the impacts of explosive contamination on vegetation is key to understanding explosives behavior in the environment. I quantified shrub growth responses to 1,3,5-trinitroperhydro-1,35-triazine (RDX) soil contamination across three life stages and I hypothesized that RDX would have the greatest impact on seed germination. Morella cerifera seeds were germinated on soils amended with RDX up to 1500 mg RDX kg-1 dry soil. Juvenile and adult individuals were exposed for 6 weeks to soil amended with RDX up to 750 and 1500 mg RDX kg-1 dry soil, respectively. Morphological responses were quantified for juveniles while physiological measurements were quantified for adults. …

Functional And Biochemical Consequences Of Single Nucleotide Polymorphisms In The Human Vesicular Monoamine Transporter 1 Gene (Slc18a1) By Sally Gamal Shukry, B.S., Sally Gamal Shukry

Theses and Dissertations

Abstract FUNCTIONAL AND BIOCHEMICAL CONSEQUENCES OF SINGLE NUCLEOTIDE POLYMORPHISMS IN THE HUMAN VESICULAR MONOAMINE TRANSPORTER 1 GENE (SLC18A1) By Sally Gamal Shukry, B.S. A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Biology at Virginia Commonwealth University. Virginia Commonwealth University, 2012 Major Advisor: Jennifer K. Stewart Associate Professor and Graduate Director, Department of Biology Single nucleotide polymorphisms (SNP) in the human VMAT1 gene (SLC18A1) have been associated with schizophrenia in three different populations: Han Chinese, Western European and Japanese. Effects of these mutations on transport function of the hVMAT1 protein have not …

Wildlife Camera Carnivore Survey: Santa Margarita Ranch, Ca, Christina Mcadams

Natural Resources Management and Environmental Sciences

The Santa Margarita Wildlife Carnivore Survey was designed to study the diversity of wildlife of the Santa Margarita Ranch, and to provide an interface with the Learning Among the Oaks (LATO) outdoor education program. The study took place in the coastal range of California, United States, and was aimed at addressing species specific analysis including habitat utilization, latency to detection, and activity patterns. Target species included native carnivores (mountain lion, bobcats, coyotes) and non­native species of management concern (feral pigs, red fox). Working nights and camera efficiency were also compared between camera models. Six Cuddeback cameras were deployed on Santa …

Alteration Of Protein Kinases' Activities By Hypoxic Mimic Compounds, Lawerence Rasmussen

Theses

No abstract provided.

Bloom-Forming Cyanobacteria And Other Phytoplankton In Northern New Jersey Freshwater Bodies, Matthew J. Rienzo

Theses

No abstract provided.

Genetic Variation In Potentially Virulent Vibrio Parahaemolyticus From The Northern Gulf Of Mexico, Nicholas Felix Noriea Iii

Dissertations

Vibrio parahaemolyticus (V_p) is a gram-negative bacterium found naturally in marine and estuarine environments. V_p is found in oysters including those which are later consumed by the public. Sub-populations of potentially virulent Vp contain specific virulence factors and are relevant human pathogens capable of causing gastroenteritis, wound infection, and death. The tdh and trh genes, both encoding hemolysins, have been correlated with the majority of clinical V_p isolates but have not been shown to be the definitive virulence factors.

A total of 146 V_p isolates from the northern Gulf of Mexico were collected and probed …

Smooth Muscle-Specific Expression Of Calcium-Independent Phospholipase A2 (Ipla2 ) Participates In The Initiation And Early Progression Of Vascular Inflammation And Neointima Formation, Shu Liu, Zhongwen Xie, Qingwei David Zhao, Huan Pang, John Turk, Lindsay E. Cormier, Wen Su, Guogang Zhao, Haifei Xu, Ming Gong, Zhenheng Guo

Biological Sciences Faculty and Staff Research

Background: The role of iPLA 2 β as a regulator of inflammatory signaling and neointima formation is unknown.

Result: Smooth muscle-specific expression of iPLA 2 β exacerbates proinflammatory cytokine production, macrophage infiltration, and neointima formation.

Conclusion: Smooth muscle-specific iPLA 2 β participates in the initiation and early progression of vascular inflammation and neointima formation.

Significance: iPLA 2 β may represent a novel therapeutic target for attenuating vascular inflammation and restenosis.

Comparison Of Creel Survey Data To Traditional Sampling Techniques In Pit-Lake Fisheries Of Muhlenberg County, Kentucky, Derek L. Rupert

Masters Theses & Specialist Projects

Populations of largemouth bass, Micropterus salmoides, and bluegill, Lepomis macrochirus, were evaluated from five pit-lakes in Muhlenberg County, Kentucky, to determine if accurate proportional stock density (PSD) data can be obtained from a mandatory creel survey. It was hypothesized that the proportion of stock-to-quality (300-400mm) and quality (+400mm) largemouth bass from four years (2007-2010) of creel survey data would be statistically similar to those generated through on-site sampling in 2011. Fish were collected via a combination of gill netting, seining, hook-and-line fishing, and boat-mounted electro-fishing. In two of the pit-lakes, the sampling-generated length frequency data was not significantly …

The Prevalence Of Essential Hypertension In Kasigau, Kenya, Lindsay Williams

Mahurin Honors College Capstone Experience/Thesis Projects

Hypertension is the leading cause of cardiovascular disease worldwide. Cardiovascular disease (CVD) is a widespread chronic non-communicable disease (NCD) which is on the rise in developing countries. Evidence based on extensive research studies on risk factors for NCDs suggests that they could be easily significantly decreased by simply controlling their risk factors. Although high blood pressure has been recognized as a leading risk factor for CVD, little research has been done to document the prevalence and incidence of essential hypertension (EH) in lower socioeconomic developing countries. One such country is Kenya. It was found in our research study that Kasigau …