Biology Commons^™

The Effects Of Climate Change And Overexploitation On Birds And Mammals: Phenological Shifts, Range Shifts, And Population Changes, Rachel Denny

Honors Theses

Our world is changing more than many individuals could imagine. Climate change is not a hoax. Comprehensive empirical records of changes since 1900 in local, regional, and global temperature clearly indicate that Earth is warming more rapidly than ever in recorded history. In response, some bird species are already shifting, both phenologically (timing) and geographically. However, the majority of birds are not shifting or not shifting fast enough, and this is resulting in population declines. A much smaller number of studies suggest that mammals are also shifting phenologically and geographically.

If we do not alter our current behaviors soon, Earth …

Role Of Epidermal Growth Factor-Triggered Pi3k/Akt Signaling In The Migration Of Medulloblastoma-Derived Cells, Veronica Dudu, Richard A. Able, Jr., Veronica Rotari, Qingjun Kong, Maribel Vazquez

Publications and Research

Medulloblastoma (MB) is the most common brain cancer diagnosed among children. The cellular pathways that regulate MB invasion in response to environmental cues remain incompletely understood. Herein, we examine the migratory response of human MB-derived Daoy cells to different concentration profiles of Epidermal Growth Factor (EGF) using a microfluidic system. Our findings provide the first quantitative evidence that EGF concentration gradients modulate the chemotaxis of MB-derived cells in a dose-dependent manner via the EGF receptor (EGF-R). Data illustrates that higher concentration gradients caused increased number of cells to migrate. In addition, our results show that EGF-induced receptor phosphorylation triggered the …

Marine Fungi Of U.S. Gulf Of Mexico Barrier Island Beaches: Biodiversity And Sampling Strategy, Allison Kathleen Walker

Dissertations

Marine fungi are an important but often overlooked component of marine ecosystems. Primarily saprotrophic, they are vital to coastal nutrient cycling processes and food webs. However, basic marine fungal distribution data are lacking in many parts of the world, as is knowledge of the sampling intensity required to characterize the biodiversity of these communities. The roles of substrate, season and latitude in shaping intertidal ascomycete community structure were examined for the U.S. Gulf of Mexico, and the role of sampling frequency on species richness estimates was also addressed. Best sampling practices were developed and 750 collections of beach detritus, sand …

Management Of Biological And Chemical Constituents For The Advancement Of Intensive, Minimal-Exchange, Biofloc-Based Shrimp (Litopenaeus Vannamei) Aquaculture, Andrew James Ray

Dissertations

Intensive, minimal-exchange, biofloc-based shrimp aquaculture systems may provide a sustainable alternative to traditional shrimp culture. Through a series of experiments, this document explores the effects of several key management strategies on water quality, isotopic distribution, and shrimp production.

An experiment evaluated the effects of managing suspended solids (biofloc) concentration at two levels. It was found that using a higher flow rate to larger settling chambers resulted in significantly lower biofloc and nitrate concentrations, and significantly improved shrimp growth rate. A second experiment compared systems with clear water and systems with biofloc. The filters in the clear water systems prevented biofloc …

Secondary Structure, A Missing Component Of Sequence- Based Minimotif Definitions, David P. Sargeant, Michael R. Gryk, Mark W. Maciejewsk, Vishal Thapar, Vamsi Kundeti, Sanguthevar Rajasekaran, Pedro Romero, Keith Dunker, Shun-Cheng Li, Tomonori Kaneko, Martin Schiller

Life Sciences Faculty Research

Minimotifs are short contiguous segments of proteins that have a known biological function. The hundreds of thousands of minimotifs discovered thus far are an important part of the theoretical understanding of the specificity of protein-protein interactions, posttranslational modifications, and signal transduction that occur in cells. However, a longstanding problem is that the different abstractions of the sequence definitions do not accurately capture the specificity, despite decades of effort by many labs. We present evidence that structure is an essential component of minimotif specificity, yet is not used in minimotif definitions. Our analysis of several known minimotifs as case studies, analysis …

Differential Expression Of Alpha 4 Integrins On Effector Memory T Helper Cells During Bordetella Infections. Delayed Responses In Bordetella Pertussis, Tzvia Abramson, Tuan M. Nguyen, Dipti Ravindra, Brian Kwong, Sana Waheed, Ryan Ferguson, Nicole Tarlton, Victoria Wu, Christopher S. Sequeira, Martina Bremer

Faculty Publications, Biological Sciences

Bordetella pertussis (B. pertussis) is the causative agent of whooping cough, a respiratory disease that is reemerging worldwide. Mechanisms of selective lymphocyte trafficking to the airways are likely to be critical in the immune response to this pathogen. We compared murine infection by B. pertussis, B. parapertussis, and a pertussis toxin-deleted B. pertussis mutant (BpΔPTX) to test the hypothesis that effector memory T-helper cells (emTh) display an altered pattern of trafficking receptor expression in B. pertussis infection due to a defect in imprinting. Increased cell recruitment to the lungs at 5 days post infection (p.i.) with B. parapertussis, and to …

Species Shout-Outs From Abdul To Zebra: Encouraging Nonmajors To Communicate In The Biology Classroom, Christina P. Colon

Publications and Research

No abstract provided.

Influences Of A Cladophora Bloom On The Diets Of Amblema Plicata And Elliptio Dilatata In The Upper Green River, Kentucky, Jennifer Maria Yates

Masters Theses & Specialist Projects

Freshwater mussels are the most imperiled group of freshwater invertebrates globally. Recent research suggests a better understanding of mussel feeding ecology may facilitate and improve conservation efforts. The use of stable isotopes is becoming an increasingly common method to study aquatic food webs. Carbon (C) and nitrogen (N) are two of the most frequently employed elements in food web studies. Differences in natural abundance of ¹³C/¹²C can indicate which food sources are the basal sources of carbon incorporated into a consumer’s tissue, while the ratio of ¹⁵N /¹⁴N provides a method of assessing trophic …

Ecologists Engaging With Communities And Regional Decision-Makers: Organized Oral Session 14 At The 2011 Esa Annual Meeting In Austin, Texas, Nadine Lymn, George Middendorf

Department of Biology Faculty Publications

No abstract provided.

A Symbiotic View Of Life: We Have Never Been Individuals, Scott F. Gilbert, J. Sapp, A. I. Tauber

Biology Faculty Works

The notion of the "biological individual" is crucial to studies of genetics, immunology, evolution, development, anatomy, and physiology. Each of these biological subdisciplines has a specific conception of individuality, which has historically provided conceptual contexts for integrating newly acquired data. During the past decade, nucleic acid analysis, especially genomic sequencing and high-throughput RNA techniques, has challenged each of these disciplinary definitions by finding significant interactions of animals and plants with symbiotic microorganisms that disrupt the boundaries that heretofore had characterized the biological individual. Animals cannot be considered individuals by anatomical or physiological criteria because a diversity of symbionts are both …

Review Of "Fifty Animals That Changed The Course Of History" By E. Chaline, John B. Jenkins

Biology Faculty Works

No abstract provided.

Nullomer Derived Anticancer Peptides (Nullops): Differential Lethal Effects On Normal And Cancer Cells In Vitro, Abdelkrim Alileche, Jayita Goswami, William Bourland, Mike Davis, Greg Hampikian

Biology Faculty Publications and Presentations

We demonstrate the first use of the nullomer (absent sequences) approach to drug discovery and development. Nullomers are the shortest absent sequences determined in a species, or group of species. By identifying the shortest absent peptide sequences from the NCBI databases, we screened several potential anti-cancer peptides. In order to improve cell penetration and solubility we added short poly arginine tails (5Rs), and initially solubilized the peptides in1M trehalose. The results for one of the absent sequences 9R (RRRRRNWMWC), and its scrambled version 9S1R (RRRRRWCMNW) are reported here. We refer to these peptides derived from nullomers as PolyArgNulloPs. A control …

Artifacts And Noise In Dna Profiling, Dan E. Krane

Biological Sciences Faculty Publications

No abstract provided.

Modulation Of The Host Response To Tacaribe Arenavirus Infection In Ag129 Mice By My-24, Eric Sefing

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

MY-24 is a new antiviral compound recently shown to protect immunocompromised mice from lethal challenge with Tacaribe virus (TCRV). Tacaribe virus is incapable of causing disease to humans, but is closely related to the highly pathogenic New World arenaviruses that cause often-fatal viral hemorrhagic fever syndromes. Remarkably, MY-24 prevents mortality without reducing virus burden in the circulation or tissues. To investigate the mechanism by which MY-24 protects AG129 mice against Tacaribe virus infection, we first characterized the natural history of disease in the model with an emphasis on host immune response and blood vessel function to establish the best times …

Dna Technology In Court, Dan E. Krane

Biological Sciences Faculty Publications

No abstract provided.

Characterization Of Fhua 104/149c: A Double Cysteine Fhua Mutant With Normal Binding And Diminished Transport, Ada K. Hagan

Electronic Theses and Dissertations

Iron is an essential element for most bacteria and is commonly acquired by siderophores, molecules secreted under iron restricted environment to bind ferric iron. Gram negative cells actively uptake these complexes via outer membrane-transport proteins such as FhuA in Escherichia coli. Structural analysis of receptors revealed a conserved β-barrel occluded by an N-terminal plug domain. The cell membrane TonB/ExbB/ExbD complex presumably supplies energy via interaction between the FhuA N-terminal TonB box and the C-terminal domain of TonB. In order to better understand the mechanism of action the FhuA mutant 104/149C, tethering the central β-strands 4 and 6 of the …

The Effects Of Temperature On Energy Utilization By Marine Invertebrate Larvae, Elizabeth Whitehill

All Dissertations

Most ectothermic organisms are larger when reared at lower temperatures and temperature can greatly affect an organism's physiological state, so understanding how temperature affects larval size and quality can provide insight into how the larval thermal environment affects post-metamorphic success. Because quality is often defined in terms of size and energy content, I focused on how temperature affects energy utilization by larvae and how differences in energy accumulation and utilization may carry over into the juvenile stage. Marine invertebrate larvae are often classified into two groups: larvae from smaller eggs that must obtain food from the environment in order to …

Optimizing Aplysia Californica's Living Conditions, Ruiying Xu

Intersections Fall 2020

The marine mollusk Aplysia californica is an important model organism for neuroscience research. In laboratory aquaria, animal waste may accumulate and harm animals unless water is replaced frequently. To keep animals healthy for longer, we have designed a marine environment that replicates the natural nitrogen cycle. Our animals' holding tanks have two compartments: a main compartment for animals and a refugium for macroalgae. We are studying whether ammonia and nitrate derived from animal waste cause illness in Aplysia, and whether macroalgae can sequester nitrates and reverse illness. We measured increases in nitrate and ammonia in the absence of macroalgae. We …

Determination Of Potential Azotobacter Vinelandii Bacteriophage Gene Sequences: A Bioinformatics Approach, Anish Reddy, Sarah Bagby

Intersections Fall 2020

Microbial communities play a key role in shaping many diverse ecosystems through their biogeochemical contributions. These communities comprise not only bacteria and archaea but also their viruses, whose reproduction profoundly affects host cell biology. While many bacterial species have been well characterized, the challenges of isolation and sequencing have hampered the study of environmental viruses such as bacteriophages. The model organism Azotobacter vinelandii is a common nitrogen-fixing soil bacterium. To our knowledge, no Azotobacter phages currently exist in culture. However, modern bioinformatic and database approaches can be used to identify the metagenomic sequences that may derive from bacteriophages that infect …

Changes In Meiotic Crossover Distribution And Frequency In Response To Chromosome Structural Variants, Christiana Wang, Nicole Crown

Intersections Fall 2020

Meiosis is an important mechanism that generates genetic variation for sexually reproducing organisms through recombination. In order for an organism to successfully propagate its genetic material from one generation to the next, its genome must be properly delivered as gametes. Meiotic crossing over of DNA ensures proper homolog segregation by repairing programmed double-stranded breaks. Meiotic defects caused by chromosome structural variants are detrimental to reproduction. During the interchromosomal effect, heterozygous inversions suppress crossing over between affected chromosomes while increasing crossing over between normal chromosome pairs. These defects in chromosomal dynamics trigger the pachytene checkpoint, leading to a delay in prophase …

Behavioral Observations Surrounding A Diet Change Of Zoo-Housed Matschie's Tree Kangaroos (Dendrolagus Matschiei), Sarah Miller, Noah T. Dunham, Diana Koester

Intersections Fall 2020

The diet of zoo-housed animals has significant implications to many aspects of each species' health and survival. A recent study revealed the diet of free-ranging Matschie's tree kangaroos (Dendrolagus matschiei) to be mostly browse-focused, including plants like leaves, ferns, and orchids. Prior to those findings, zoo-housed tree kangaroos were fed diets, mostly biscuit-based, that differ vastly from that of free-ranging tree kangaroos possibly contributing to widespread issues within zoos concerning obesity and low reproductive success. The Cleveland Metroparks Zoo (CMZ) houses two Matschie tree kangaroos which, in 2019, underwent a significant diet change from a more traditional, baseline, biscuit-heavy diet …

Wheelchair Athlete Concussion Baseline Data: A Pilot Retrospective Analysis, Jonathan Lee, Michael Harper, Michael Uihlein, Kenneth Lee

Intersections Fall 2020

After a thorough literature search and through experience at adaptive sports events, we identified a lack of established guidelines for Sport-related concussion (SRC) evaluation and management in wheelchair athletes. Wheelchair athletes are a unique population, and certain conventional methods of evaluating SRC in able-bodied sports cannot be used. For example, wheelchair athletes' underlying disabilities restrict the use of standard balance testing protocols. In addition, some wheelchair athletes have concurrent traumatic brain injuries and may have baseline cognitive deficits associated with their disability that mimic the symptoms of a concussion. This research focused on discussing current concussion management guidelines in Adaptive …

Does Diet Breadth Resolve Variation In Climate Driven Range Shifts Of Ohio Butterflies?, Lydia Beera, Angie Lenard, Sarah E. Diamond

Intersections Fall 2020

As a result of recent climate change, many species are rapidly altering their geographic ranges, in a phenomenon known as range shifting. In general, the shifts tend to be geographically poleward or upslope, but there is a lot of variation in the magnitude or direction of contemporary range shifts. Theory suggests that specialization might constrain range shift responses. In this experiment, we examined the relationship between the magnitude and direction of range shift responses and degree of diet specialization of Ohio butterfly larvae. We used a long-term monitoring dataset of butterfly range dynamics to test this relationship. Surprisingly, we did …

Why Snails? How Gastropods Improve Our Understanding Of Ecological Disturbance, Christopher P. Bloch

Bridgewater Review

The concept of equilibrium - the idea that a perturbed system will tend to return to its original state - is the basis for many foundational theories in ecology. Yet, the spatial and temporal dynamics of ecosystems are strongly influenced by disturbance. If a particular disturbance greatly alters local climatic conditions, gastropods should be among the first organisms to show a measurable response. The effects of human alteration of habitats (for example, conversion of forest to agriculture) have much longer-lasting effects than those of natural disturbances.

Relative Stability Of Plant Communities In A South Carolina High Salt Marsh, James O. Luken

Biology

The high marsh in southeast Atlantic coast salt marshes forms a relatively small but ecologically important transition zone between low marsh and the terrestrial shoreline. However, long-term trend data from high marshes are limited to a few studies. Permanent plots established in a high marsh near Waties Island in northeast South Carolina were measured for plant coverage from 2002-2010. At the beginning and at the end of the study, four groups of plots were identified: mixed indicated by Borrichia frutescens, Distichlis spicata, and Fimbristylis castanea; Juncus indicated by Juncus roemerianus; Salicornia indicated by Salicornia virginica; and Spartina indicated by Spartina …

When Dna Won't Work, Rick Visser, Greg Hampikian

Biology Faculty Publications and Presentations

Within the criminal justice system, DNA (deoxyribonucleic acid) evidence has often been heralded as the gold standard of forensic science. In a 2009 U.S. Supreme Court decision, Chief Justice Roberts wrote that "DNA testing has an unparalleled ability both to exonerate the wrongly convicted and to identify the guilty. It has the potential to significantly improve both the criminal justice system and police investigative practices." The phrases “unparalleled ability” and “significantly improve” reflect the high standard that DNA has attained in both forensic science and the entire criminal justice system.

Forensic DNA technology has a major advantage over other forensic …

Influence Of Nitrogen And Phosphorus On Ecosystem Co2 Exchange In A Restored Temperate Grassland, Lynn M. Socha

Biology Theses

Nitrogen (N) and phosphorus (P) affect the structure and function of grasslands by altering plant competitive interactions, shifting patterns of above- and belowground biomass allocation, and by increasing net primary production. However, the influence of N and P on net ecosystem productivity is poorly understood. In the context of a field-based factorial N- and P-addition experiment, I measured soil moisture, leaf area index and component fluxes of ecosystem CO₂ exchange throughout the growing season in a restored temperate grassland near Buffalo, New York. I predicted that N-addition would enhance ecosystem CO₂ uptake (GEE) while P-addition would stimulate ecosystem …

Statistical Weights Of Single Source Dna Profiles, Dan E. Krane

Biological Sciences Faculty Publications

No abstract provided.

Generating Forensic Dna Profiles, Dan E. Krane

Biological Sciences Faculty Publications

No abstract provided.

What Can Go Wrong With Dna Profiling, Dan E. Krane

Biological Sciences Faculty Publications

No abstract provided.