Biology Commons^™

Identification And Characterization Of Equine Rotaviruses In Southern Ireland, Crystal Cronin

Theses

Group A Rotaviruses(GARVs) are enteric pathogens which induce acute gastroenteritis in infants, neonates and the young of many animal species. During a 4 year study (2005-2009), 64 fecal samples from symptomatic foals, from several counties, in the south of Ireland, were molecularly characterized to determine the VP& (G type), VP4 (P type), and NSP4 antigenic specificities. Globally, G3 [P12] and G14[P12] are recognized as the predominant equine GARV strains. Twenty-two samples (34.38%) were found to contain G3 viruses, while 21 samples (32.81%) contained G14 viruses. In this study, there was a distinct increase in the incidence of mixed infections circulating …

Protein Expression Of Hypoxia-Inducible Factor 1-Alpha (Hif-1Α) In Spot (Leiostomus Xanthurus) Under Acute Exposure To Hypoxic Treatments : A Laboratory-Field Comparison Study, Mason James Smith

UNF Graduate Theses and Dissertations

Hypoxia in coastal estuaries is a topic of increasing concern, as the magnitude and frequency of hypoxic events have increased over the past several decades. These hypoxic events are highly detrimental to the coastal biota, particulary fish. The hypoxia-inducible factor 1α (HIF-1α) protein was used as a candidate biomarker for deciphering exposure of fish to hypoxic events. In chapter one, Spot (Leiostomus xanthurus) were exposed to three treatment groups of DO (means 7.32 ± 0.18, 5.15 ± .37, and 2.57 ± 0.01 mg Lˉ¹ DO) and sampled (n = 5) at time zero, 36 and 72-h for each treatment. The …

Localization And Characterization Of The Interactions Between Basigin Gene Products And Monocarboxlate Transporters In The Olfactory Bulb Of The Mouse, Paul L. Gambon

UNF Graduate Theses and Dissertations

Basigin, the mouse form of the human protein EMMPRIN, is commonly found as a transmembrane homodimer with carboxy termini in the cytosol and extracellular amino-termini. Because of its important role as a cell-to-cell junction molecule, and possible implications for cancer research, a Basigin null mouse was developed in 1996 by Igakura et al., to aid in the study of this protein. Their early research demonstrated that Basigin plays a large role in embryonic development. Mice lacking the Basigin gene are blind from the time of eye opening, and have demonstrated a lack of aversion to offensive odors such as acetic …

Invasive Fish Effects On The Invertebrate Constituents Of Wetland Communities, Levi Bridges

EWU Masters Thesis Collection

The purpose of this research was to assess the impacts of invasive fishes on wetland communities. Within this study we explored fish effects on the invertebrate constituents of these wetlands, and possible niche overlap of fish and dabbling waterfowl using stable isotope analysis. Brook stickleback (Culaea inconstans) and pumpkinseed (Lipomis gibbosus) pose a potentially growing threat to temperate Pacific drainages of North America due to their ability to outcompete native fauna. Our study compares and contrasts the invertebrate constituents, their diversity, abundances, and stable isotope ecology among fish invaded and non-invaded wetlands. We found that brook stickleback and pumpkinseed are …

Engineering Proteins With Unique Characteristics For Diagnostics And Biosensors, Smita Joel

University of Kentucky Doctoral Dissertations

Proteins possess a broad range of structural and functional properties and, therefore, can be employed in a variety of biomedical applications. While a good number of protein-based biosensing systems and biosensors for target analytes have been developed, the search for versatile, highly sensitive and selective sensors with long term stability able to provide fast detection of target analytes continues to be a challenge. To that end, we now report the design and development of modified proteins with tailored characteristics and their further utilization in the development of biosensing systems.

We take advantage of binding proteins that undergo a change in …

Efficacy Of Constructed Wetlands Of Various Depths For Natural Amphibian Community Conservation, Andrea Nicole Drayer

Online Theses and Dissertations

Wetlands provide critical habitat for a diverse group of amphibians and provide important ecosystem functions and services to humans. Despite this, most natural wetlands have been lost to land use practices. Consequently, constructing wetlands has become a common practice to mitigate for removed wetlands and to manage for wildlife. There were three primary objectives of this research: 1) to examine whether or not constructed wetlands located on ridge tops in eastern Kentucky in the Daniel Boone National Forest (DBNF) had amphibian communities comparable to natural ephemeral wetlands, 2) to examine amphibian predator-prey relationships within the constructed wetlands, and 3) to …

A Post-Restoration Assessment Of The Fishes And Macroinvertebrates Of Mill Branch, Knox County, Kentucky, With Emphasis On The Blackside Dace (Chrosomus Cumberlandensis), Dwayne Kevin Merrill

Online Theses and Dissertations

The first stream habitat restoration project for the federally threatened blackside dace, Chrosomus cumberlandensis (Starnes and Starnes), was initiated in fall 2005 on Mill Branch, a small headwater stream located in the Upper Cumberland River basin, Knox County, Kentucky. The lower 700 meters of Mill Branch were restored through construction of a new, stable channel with specially designed habitat features for blackside dace. In August 2009, the newly restored channel was connected to the mainstem and the old channel was eliminated. A post-restoration survey was conducted on the fishes (with emphasis on the blackside dace) and benthic macroinvertebrate communities, between …

Ultraviolet Reflectance Of Nestling Plumage And The Provisioning Strategies Of Adult Eastern Phoebes (Sayornis Phoebe), Alyssa Rae Underwood

Online Theses and Dissertations

Most songbirds are visually sensitive to ultraviolet (UV) wavelengths and, in some species, variation in the extent to which plumage reflects in the UV range provides information about individual quality that influences mate-choice decisions. Less is known about the possible importance of plumage UV reflectance in parent-offspring relationships. The lower breast and belly plumage of nestling Eastern Phoebes (Sayornis phoebe) reflects in the UV and adults may use variation in this reflectance to evaluate nestling quality and vary their provisioning behavior accordingly. To examine this possibility, I manipulated UV reflectance of nestling plumage by applying preen oil that …

Land Snail Species Diversity And Composition Between Different Forest Disturbance Regimes In Central And Eastern Kentucky Forests, Daniel Douglas

Online Theses and Dissertations

Land snails are cornerstone organisms that contribute to properly functioning ecosystems. However, habitat loss and destruction have led to these organisms being one of the most imperiled groups on the planet. Due to their relatively sedentary nature, land snails can be susceptible to anthropogenic disturbance and habitat fragmentation. Because of this, old-growth forests have the potential for being premiere habitat for these organisms, and snails have the potential to be good indicators of old-growth habitats. This study compared land snail species diversity and community composition in old-growth and second-growth forests in the Inner Bluegrass, Cumberland Plateau, and Pine Mountain ecoregions …

Effect Of Nestling Sex Ratio On The Provisioning Behavior Of Adult Eastern Bluebirds (Sialia Sialis), Barbara Ellen Kieffer

Online Theses and Dissertations

To maximize reproductive success, parents may, in some cases, differentially invest in sons and daughters, i.e., sex-biased parental investment. Preferential provisioning behavior has been reported in one population of Eastern Bluebirds (Sialia sialis) and attributed to local resource competition. To better understand this behavior, I studied the provisioning behavior of Eastern Bluebirds in Madison County, Kentucky, in 2004. I experimentally manipulated brood sex ratios in 24 bluebird nests, creating female-biased (N = 8), male-biased (N = 5), and control (N = 11) nests. Following manipulation, nests were video-taped to record adult provisioning behavior. Among experimental broods, the provisioning rates …

Amphibian Community Similarity Between Natural Ponds And Constructed Ponds Of Multiple Types In Daniel Boone National Forest, Kentucky, Robert Daniel Denton

Online Theses and Dissertations

Amphibians are in a worldwide decline. Among the many causes for amphibian declines, habitat loss and alteration remains one of the most significant. A lack of federal protection for isolated wetlands that provide habitat for unique species has resulted in the loss of breeding habitat and unregulated mitigation practices. Ponds built for mitigation purposes often do not replicate the lost ponds in structure or ecological processes. A lack of general monitoring has produced a void in knowledge of what long-term role constructed ponds play in shaping amphibian communities. My objective was to compare amphibian communities of natural ponds and multiple …

Survival Rate Of C. Elegans, Kimberly Fletcher

Summer Community of Scholars Posters (RCEU and HCR Combined Programs)

No abstract provided.

Protein-Ligand Docking Using The Autodock Suite Of Programs, Tripp A. Lamb

Summer Community of Scholars Posters (RCEU and HCR Combined Programs)

No abstract provided.

Infection Rate Of Dactylogyrus Parasites On Etheostoma Duryi And Etheostoma Zonale, Kara Million

Summer Community of Scholars Posters (RCEU and HCR Combined Programs)

No abstract provided.

Recombinant Expression, Protein Purification, And Crystallization Of Thermococcus Thioreducens Str, Ogl-20, Michelle Morris

Summer Community of Scholars Posters (RCEU and HCR Combined Programs)

No abstract provided.

Resource Assessment Framework (Raf) For Finfish Resources In Western Australia, Department Of Fisheries

Fisheries Occasional Publications

No abstract provided.

Short Peptides As Inhibitors Of Amyloid Aggregation, Bradley Neddenriep, Anastasia Calciano, Daniel Conti, Erin Sauve, Marissa Paterson, Edward Bruno, David A. Moffet

Chemistry and Biochemistry Faculty Works

The misfolding and aggregation of proteins into amyloid has been linked to a variety of age-related diseases. Aggregation of proteins, such as Aβ in Alzheimer's disease and Islet Amyloid Polypeptide (IAPP, amylin) in type 2 diabetes, appears to lead to the formation of toxic assemblies. These assemblies range in size from small oligomers (2-8 proteins) to large fibrils (thousands of proteins). It remains unclear how these amyloidogenic proteins misfold and form toxic species, but growing evidence suggests that inhibiting the aggregation of these proteins could slow, if not prevent altogether, the progression of these diseases. We describe the use of …

Relationships Between Physiological Characteristics And Trace Metal Body Burdens Of Banded Garden Spiders Argiope Trifasciata (Araneae, Araneidae), Martina Ramirez, Jeremy Mccallum, James M. Landry, Viviana A. Vallin, Sarah H. Fukui, Heidi E. Gergus, Jaclyn D. Torres, Charisse L. Sy

Biology Faculty Works

Banded garden spiders (Argiope trifasciata) were collected at the Ballona Wetlands, a metal contaminated salt marsh. The relationship between spider body size and individual metal loads was investigated. Biochemical markers were identified in spider fecal material and found to correlate to body metal levels. Body metal dry weight concentrations of Cd, Cr, Cu, Zn and total metals in female A. trifasciata exhibited distinct patterns of spatial and annual variation during 2006 and 2007. Spider body size was homogeneous across sites in both years, while increased Cd and Cr concentrations were sometimes associated with a reduction in spider size, though the …

An Evaluation Of Habitat Structure And The Distribution Of Rare And Common Darters In Ohio, Erin Lee Kingdom

Browse all Theses and Dissertations

Darters are small benthic-feeding fish. I examined reasons why some darter species are rarer than others based on geographic range, habitat specificity, and local population size using the Ohio EPA database and field research. The Qualitative Habitat Evaluation Index (QHEI) represented habitat quality. I examined drainage area and gradient at the landscape-scale, riparian and channel characteristics at the reach-scale, and substrate, cover, and riffle/pool characteristics at the microhabitat scale. Some rare species occur in few rivers in Ohio, but throughout a basin, while other rare species occur only in moderate-sized rivers. Most rare species occur at only a few sites …

Changes In Avian Community Composition At Sugarcreek Metropark Between 1978 And 2010, Jennifer Lynne Hays

Browse all Theses and Dissertations

Quantifying changes in forest avian diversity is a challenging, but necessary task for development of effective conservation plans. While small changes in diversity accumulate over time, these changes do not necessarily reflect overall long-term trends in species diversity. Long-term changes from established baseline conditions may be more interpretable because the changes in diversity are assessed over longer periods. In 1978, Dr. Reed Noss (1981) initiated a study of thirty-three breeding bird censuses at Sugarcreek Reserve (Metropark) in Southwestern Ohio and evaluated the species richness and composition during the breeding and post-breeding season to inform ecological reserve design theory. In 2010, …

Multi-Locus Evidence Of A Late Pleistocene Divergence And Sex-Biased Dispersal In The North American Wood Duck (Aix Sponsa), Christopher T. Bigley

Browse all Theses and Dissertations

The Pleistocene was characterized by fluctuations in climate causing repeated advances and retreats of glacial ice. The advancing ice sheets caused habitat fragmentation which initiated population divergence and speciation events between eastern and western avian populations within northern temperate forests. Based on mitochondrial DNA (mtDNA) control region sequences, North American Wood Duck (Aix sponsa) populations fit this model of divergence. However, mtDNA is maternally inherited, and thus may not reflect the genomic history of this species, because of male biased-dispersal, selection, or stochastic lineage sorting. To test the "Late Pleistocene divergence" hypothesis, I sequenced 11 independent nuclear introns (nuDNA) for …

The Effect Of Mycorrhizal Inoculation Prior To Transplantation On Wetland Restoration Success In Sites Of Different Land Use Histories, Brett Joseph Fisher

Browse all Theses and Dissertations

One factor used to determine wetland mitigation success is the establishment of native wetland plant species. Although mycorrhizal associations are known to be present in 70 to 95% of all plant species and have been linked to seedling establishment as well as nutrient and carbon flux within plant communities, the presence or absence of mycorrhizal fungi are not assessed or addressed during wetland mitigation work. Three experiments were devised to examine the effects of mycorrhizal fungi on the germination and growth of native wetland plant species in soils and field sites from natural, restored, and created wetlands.

Greenhouse experiment I …

Soil Moisture, Fire, And Tree Community Structure, William Patrick White

Browse all Theses and Dissertations

My study was conducted to understand tree community structure and how soil moisture and fire frequency influence them. Eighteen plots were placed in the Edge of Appalachia Nature Preserve of unglaciated southern Ohio: nine within a prescribed burn site and nine control sites outside the burn. Sites were stratified in triplicate across GIS-derived integrated soil moisture index (IMI) classes. Burning was done in 1996. Overstory species dbh and sapling species were sampled 1997, 2001, and 2008. Overstory stems were located in 2009 using range finders. Stem locations were loaded into GIS using novel techniques to quantify individual stem IMI values. …

The Effects Of Forest Fragmentation On The Reproductive Success Of Spring Ephemeral Wildflowers And Their Pollinators, Holly Lynn Schlotman

Browse all Theses and Dissertations

Low reproductive output has been shown in small, isolated populations of plants, and spring wildflowers in forest fragments may show a similar pattern. The goal of this study is to determine the impact of forest fragmentation on the reproductive success of three wildflowers: Cardamine concatenata, Delphinium tricorne, and Dicentra cucullaria. A secondary goal was to determine the impact of forest fragmentation on bumblebees (Bombus spp.) since they are important spring pollinators. Correlations and stepwise regression were used to determine the effects of forest fragment size on the reproductive success of each wildflower species, and also on the abundance and diversity …

The Role Of Co-Infection In The Spread Of Hiv In Sub-Saharan Africa, Diego Fernando Cuadros

Theses and Dissertations--Biology

The cause of the high HIV prevalence in sub-Saharan Africa is incompletely understood, with heterosexual penile-vaginal transmission proposed as the main mechanism. Heterosexual HIV transmission has a very low probability; further, a single estimation of heterosexual probability of HIV transmission fails to reproduce the variation associated with important biological cofactors. In particular, studies of HIV incidence suggest that co-infection with other infectious diseases influence the HIV transmission, and therefore might substantially vary the pattern of the spread of the infection. To assess the effect of co-infection on the spread of HIV, I developed and analyzed several mathematical and statistical models …

Some Laelapine Mites (Acari: Laelapidae) Ectoparasitic On Small Mammals In The Galapagos Islands, Including A New Species Of Gigantolaelaps From Aegialomys Galapagoensis, Donald D. Gettinger, Fernanda Martins-Hatano, Scott Lyell Gardner

Scott L. Gardner Publications

A collection of laelapine mites from small mammals in the Galapagos Islands are identified and their host distributions reviewed. Two species of native rodents, Aegialomys galapagoensis and Nesoryzomys narboroughii, were infested only with species typical of Neotropical oryzomyine rodents; Rattus rattus was infested with Laelaps nuttalli, a host-specific ectoparasite to Old World Rattus. A synopsis of Gigantolaelaps Fonseca is provided and we describe a new laelapine mite, Gigantolaelaps aegialomys n. sp., from the pelage of the rodent A. galapagoensis on Santa Fe Island. The new species has strong morphological affinities with a subgroup of Gigantolaelaps associated …

Composite Effects Of Polymorphisms Near Multiple Regulatory Elements Create A Major-Effect Qtl, Ryan D. Bickel, Artyom Kopp, Sergey V. Nuzhdin

School of Biological Sciences: Faculty Publications

Many agriculturally, evolutionarily, and medically important characters vary in a quantitative fashion. Unfortunately, the genes and sequence variants accounting for this variation remain largely unknown due to a variety of biological and technical challenges. Drosophila melanogaster contains high levels of sequence variation and low linkage disequilibrium, allowing us to dissect the effects of many causative variants within a single locus. Here, we take advantage of these features to identify and characterize the sequence polymorphisms that comprise major effect QTL alleles segregating at the bric-a-brac locus. We show that natural bric-a-brac alleles with large effects on cuticular pigmentation reflect a cumulative …

Epigenetic Regulatory Mechanisms In Plants, Zoya Avramova

School of Biological Sciences: Faculty Publications

Genomes are defined by their primary sequence, which provides the genetic blueprint of a species. Eukaryotic DNA functions within the context of chromatin, which provides additional layers of gene regulation referred to as “epigenetic.” The commonly found definition of epigenetics is that of a “study of heritable changes in genome function that occur without a change in DNA sequence.” However, evidence that neuronal gene-expression states are also regulated by epigenetic mechanisms, despite evidence that neuronal cells do not divide, has opened space for a broader unifying definition that keeps “the sense of prevailing usage but avoids constraints imposed by stringently …

Sex Chromosome-Specific Regulation In The Drosophila Male Germline But Little Evidence For Chromosomal Dosage Compensation Or Meiotic Inactivation, Colin D. Meiklejohn, Emily L. Landeen, Jodi M. Cook, Sarah B. Kingan, Daven C. Presgraves

School of Biological Sciences: Faculty Publications

The evolution of heteromorphic sex chromosomes (e.g., XY in males or ZW in females) has repeatedly elicited the evolution of two kinds of chromosome-specific regulation: dosage compensation—the equalization of X chromosome gene expression in males and females— and meiotic sex chromosome inactivation (MSCI)—the transcriptional silencing and heterochromatinization of the X during meiosis in the male (or Z in the female) germline. How the X chromosome is regulated in the Drosophila melanogaster male germline is unclear. Here we report three new findings concerning gene expression from the X in Drosophila testes. First, X chromosome-wide dosage compensation appears to be absent from …

Arabidopsis G-Protein Interactome Reveals Connections To Cell Wall Carbohydrates And Morphogenesis, Karsten Klopffleisch, Nguyen Phan, Kelsey Augustin, Robert S. Bayne, Katherine S. Booker, Jose R. Botella, Nicholas C. Carpita, Tyrell Carr, Jin-Gui Chen, Thomas Ryan Cooke, Arwen Frick-Cheng, Erin J. Friedman, Brandon Fulk, Michael G. Hahn, Kun Jiang, Lucia Jorda, Lydia Kruppe, Chenggang Liu, Justine Lorek, Maureen C. Mccann, Antonio Molina, Etsuko N. Moriyama, M. Shahid Mukhtar, Yashwanti Mudgil, Sivakumar Pattathil, John Schwarz, Steven Seta, Matthew Tan, Ulrike Temp, Yuri Trusov, Daisuke Urano, Bastian Welter, Jing Yang, Ralph Panstruga, Joachim F. Uhrig, Alan M. Jones

School of Biological Sciences: Faculty Publications

The heterotrimeric G-protein complex is minimally composed of Ga, Gb, and Gc subunits. In the classic scenario, the G-protein complex is the nexus in signaling from the plasma membrane, where the heterotrimeric G-protein associates with heptahelical G-protein-coupled receptors (GPCRs), to cytoplasmic target proteins called effectors. Although a number of effectors are known in metazoans and fungi, none of these are predicted to exist in their canonical forms in plants. To identify ab initio plant G-protein effectors and scaffold proteins, we screened a set of proteins from the G-protein complex using two-hybrid complementation in yeast. After deep and exhaustive interrogation, we …