Biology Commons^™

Myosin Vi Regulates Actin Structure Specialization Through Conserved Cargo-Binding Domain Sites, Mamiko Isaji, Marta Lenartowska, Tatsuhiko Noguchi, Deborah J. Frank, Kathryn G. Miller

Biology Faculty Research

Actin structures are often stable, remaining unchanged in organization for the lifetime of a differentiated cell. Little is known about stable actin structure formation, organization, or maintenance. During Drosophila spermatid individualization, long-lived actin cones mediate cellular remodeling. Myosin VI is necessary for building the dense meshwork at the cones' fronts. We test several ideas for myosin VI's mechanism of action using domain deletions or site-specific mutations of myosin VI. The head (motor) and globular tail (cargo-binding) domains were both needed for localization at the cone front and dense meshwork formation. Several conserved partner-binding sites in the globular tail previously identified …

On The Utility Of Population Models For Invasive Plant Management: Response To Evans And Davis, Eleanor A. Pardini, John M. Drake, Tiffany M. Knight

Biology Faculty Research

No abstract provided.

Will The Use Of Less Fecund Cultivars Reduce The Invasiveness Of Perennial Plants?, Tiffany M. Knight, Kayri Havens, Pati Vitt

Biology Faculty Research

Many invasive species were originally introduced for horticultural purposes, and several continue to be profitable for the green (nursery, horticulture, and landscape) industry. Recently, some plant suppliers have marketed less fecund cultivars of several invasive species, including glossy buckthorn (Frangula alnus), burning bush (Euonymus alatus), and Japanese barberry (Berberis thunbergii), as “safe” alternatives to invasive relatives. We use published matrix population models to simulate the effect of reducing fecundity on the population growth rates of invasive species. We show that large changes in fecundity result in relatively small changes to the population growth rates of long-lived species, which suggests that …

Mechanosensitive Channels: What Can They Do And How Do They Do It?, Elizabeth S. Haswell, Rob Phillips, Douglas C. Rees

Biology Faculty Research

While mechanobiological processes employ diverse mechanisms, at their heart are force-induced perturbations in the structure and dynamics of molecules capable of triggering subsequent events. Among the best characterized force-sensing systems are bacterial mechanosensitive channels. These channels reflect an intimate coupling of protein conformation with the mechanics of the surrounding membrane; the membrane serves as an adaptable sensor that responds to an input of applied force and converts it into an output signal, interpreted for the cell by mechanosensitive channels. The cell can exploit this information in a number of ways: ensuring cellular viability in the presence of osmotic stress and …

Asynchronous Expression Of The Homeodomain Protein Cux1 In Sertoli Cells And Speratids During Spermatogenesis In Mice, Melissa R. Kroll, Engela S. Viss, Jonathan Lamb, Joy Horstman, Alexander Powell, Andrea Van Wyk, Kaarlo Hinkkala, Gregory B. Vanden Heuvel, Traci Hoogland, Matthew Schippers, Stephen Shannon, Carol G. Carlton, Madhulika Sharma, Aaron Taylor, Tony N. Jelsma

Faculty Work Comprehensive List

The homeodomain CUX1 protein exists as multiple isoforms that arise from proteolytic processing of a 200-kDa protein or an alternate splicing or from the use of an alternate promoter. The 200-kDa CUX1 protein is highly expressed in the developing kidney, where it functions to regulate cell proliferation. Transgenic mice ectopically expressing the 200-kDa CUX1 protein develop renal hyperplasia associated with reduced expression of the cyclin kinase inhibitor p27. A 55-kDa CUX1 isoform is expressed exclusively in the testes. We determined the pattern and timing of CUX1 protein expression in developing testes. CUX1 expression was continuous in Sertoli cells from prepubertal …

Computational Discovery Of Phenotype Related Biochemical Processes For Engineering, Andrea M. Rocha

USF Tampa Graduate Theses and Dissertations

Application of bioengineering technologies for enhanced biological hydrogen production is a promising approach that may play a vital role in sustainable energy. Due to the ability of several naturally occurring microorganisms to generate hydrogen through varying metabolic processes, biological hydrogen has become an attractive alternative energy and fuel source.

One area of particular interest is the production of biological hydrogen in organically-rich engineered systems, such as those associated with waste treatment. Despite the potential for high energy yields, hydrogen yields generated by bacteria in waste systems are often limited due to a focus on microbial utilization of organic material towards …

A Novel Device For Cell-Cell Electrofusion, Justin T. Stewart

USF Tampa Graduate Theses and Dissertations

Cell transplantation therapy is a potentially powerful tool and can be used to replace defective cells with healthy cells. This offers the possibility of alleviating the destructive symptoms for many diseases such as Parkinson's disease, Alzheimer's disease, stroke, spinal cord trauma, Type I diabetes and many more. While there are many diseases that could be positively impacted from cell transplantation therapy, the focus of this research is insulin dependent, Type I Diabetes.

The Islets of Langerhans are composed of various types of cells located in the pancreas and are responsible for a variety of biochemical functions. Specifically, the beta Islet …

Comparison Of A Novel Fluorogenic Anti-Fxa Assay With A Central Laboratory Chromogenic Anti-Fxa Assay For Measuring Lmwh Activity In Patient Plasmas, Leanne F. Harris, Vanessa Castro-Lopez, James S. O'Donnell, Anthony J. Killard

Articles

Introduction: Low molecular weight heparins (LMWHs) are used worldwide for the treatment and prophylaxis of thromboembolic disorders. Routine laboratory tests are not required due to the predictable pharmacokinetics of LMWHs, with the exception of pregnant patients, children, patients with renal failure, morbid obesity, or advanced age. Anti-Factor Xa (anti-FXa) plasma levels are most often employed in the assessment and guidance of accurate dosing in these patient cohorts. Materials and methods: A LMWH calibration curve was generated using citrated human pooled plasma spiked with pharmacologically relevant concentrations (0–1 U/ml) of two low molecular weight heparins; enoxaparin and tinzaparin. Least squares analysis …

Evaluation Of Activated Partial Thromboplastin Time (Aptt) Reagents For Application In Biomedical Diagnostic Device Development, Magdalena Dudek, Leanne F. Harris, Anthony J. Killard

Articles

Introduction: The most commonly used test for monitoring heparin therapy is the activated partial thromboplastin time (aPTT). The response of available aPTT reagents to heparin varies significantly. The aim of this study was to highlight the differences between aPTT reagents stored in a dried format to select the most suitable formulations to be used for the development of point-of-care diagnostic devices used for monitoring of unfractionated heparin dose response. Methods: Ten reagents were analysed in terms of their performance in liquid and in dried form after storage for 24 h and 14 days. Performance was assessed by measurement of the …

The Conservation And Ecology Of Carnivorous Plants, David Jennings

USF Tampa Graduate Theses and Dissertations

As discussed in Chapter 1, although our understanding of the ecology and evolution of carnivorous plants has greatly improved in recent years, many fundamental questions remain unanswered. Unfortunately, at the present time, many carnivorous plants are increasingly threatened by anthropogenic activities. Indeed, over half of the carnivorous plant species assessed by the International Union for the Conservation of Nature (IUCN) are listed as `threatened', but the threats to carnivorous plants have not previously been quantified. In Chapter 2, I quantified the conservation threats to carnivorous plant taxa worldwide by searching peer-reviewed literature, and found data on the threats to 48 …

Seagrass Patch Dynamics In Areas Of Historical Loss In Tampa Bay, Fl, Usa, Kristen A. Kaufman

USF Tampa Graduate Theses and Dissertations

The study documents seagrass patch dynamics over large spatial extents in Tampa Bay, Florida. Using GIS techniques a set of fine scale seagrass maps was created within locations previously identified as "patchy" seagrass or areas of seagrass loss. Thirty randomly selected landscape windows of various extents were mapped for the years 2004, 2006, and 2008 by visualizing 0.3 m resolution color imagery on-screen at a digitizing scale of 1:500 using a minimum mapping unit of 1 m². Characteristics of seagrass patches and patterns of seagrass change were quantified using area-based and time interval metrics including total seagrass area, …

Underwater Hearing In The Loggerhead Turtle (Caretta Caretta): A Comparison Of Behavioral And Auditory Evoked Potential Audiograms, Kelly Martin

USF Tampa Graduate Theses and Dissertations

Methods for collecting behavioral audiograms are often time consuming and require trained, captive subjects. It is more practical to measure hearing sensitivity using electrophysiological methods, such as auditory evoked potential (AEP) testing, in which electrodes measure action potentials in response to acoustic stimuli. These data can be collected in a matter of hours. However, results should be verified through behavioral testing. Current knowledge of marine turtle auditory abilities is based on a few electrophysiological tests. The purpose of this study was to collect and compare behavioral and auditory evoked potential audiograms in a captive adult loggerhead turtle (Caretta caretta …

Effects Of Fixed Night Shift Work On 24 Hour Blood Pressure Regulation, State Anxiety Levels And Total Sleep Time, Jennifer L. Witting

Dissertations, Master's Theses and Master's Reports - Open

Nearly 22 million Americans operate as shift workers, and shift work has been linked to the development of cardiovascular disease (CVD). This study is aimed at identifying pivotal risk factors of CVD by assessing 24 hour ambulatory blood pressure, state anxiety levels and sleep patterns in 12 hour fixed shift workers. We hypothesized that night shift work would negatively affect blood pressure regulation, anxiety levels and sleep patterns. A total of 28 subjects (ages 22-60) were divided into two groups: 12 hour fixed night shift workers (n=15) and 12 hour fixed day shift workers (n=13). 24 hour ambulatory blood pressure …

Impact Of Copper Mine Tailings (Stamp Sand) On Survival And Development Of Aquatic Organisms Near Gay, Michigan, Danielle M. Haak

Dissertations, Master's Theses and Master's Reports - Open

Heavy metal-rich copper mine tailings, called stamp sands, were dumped by mining companies directly into streams and along the Lake Superior shoreline, degrading Keweenaw Peninsula waterways. One of the largest disposal sites is near Gay, Michigan, where tailings have been moved along the shoreline by currents since mining ceased. As a result, the smallest sand particles have been washed into deeper water and are filling the interstitial spaces of Buffalo Reef, a critical lake trout spawning site. This research is the first to investigate if stamp sand is detrimental to survival and early development of eggs and larvae of lake …

Omega-3 Fatty Acids And The Neurovascular Responses To Mental Stress In Humans, Christopher Elmer Schwartz

Dissertations, Master's Theses and Master's Reports - Open

Hypertension is the most prevalent form of cardiovascular disease (CVD) in the world, and is known to increase the risk for developing other diseases. Recently, the American Heart Association introduced a new classification of blood pressure, prehypertension (PHT). The criteria for PHT include a systolic of 120-139 mmHg and/or a diastolic blood pressure of 80-89 mmHg. It has been observed that individuals with PHT have a higher risk of developing hypertension later in life. Therefore, it is important to understand the mechanisms contributing to PHT in order to possibly prevent hypertension. Omega-3 fatty acids found in fish oils have been …

Response Of Riparian Plant Communities To Hydrological Alteration: Are Seed Banks A Means Of Restoration?, Siobhan Boyer Gorham

Electronic Theses and Dissertations

The effects of anthropogenic modifications of stream systems on in situ plant community composition and seed bank composition were examined at five riparian sites in the Yazoo River watershed in northern Mississippi. This analysis was performed to determine if the soil seed bank can provide a means for the restoration of riparian floodplain plant communities. The study sites represented five different kinds of hydrological alteration: a highly incised riparian terrace isolated from the stream channel, a deforested original stream with reduced flow due to channelization and standing water due to levees, a forested original stream with reduced flow due to …

Conservation Genetics And Distribution Of The Yazoo Darter (Etheostoma Raneyi), Kenneth Alan Sterling

Electronic Theses and Dissertations

The Yazoo Darter is a range-restricted endemic fish in north-central Mississippi. Because the limited and fragmented range of this species puts it at risk of extinction, the Yazoo Darter has been classified as vulnerable or sensitive by several agencies and conservation organizations. However, the actual conservation status of this species is uncertain. Information necessary for conservation management is relatively sparse and is scattered among published and unpublished sources, particularly collection records. I have consolidated all known collection records for this species into a standardized database which has not only yielded valuable information presented here, but will provide a resource for …

Growth, Mortality, And Life-History Scaling Across Species, Eric L. Charnov, Wenyun Zuo

Biology Faculty & Staff Publications

No abstract provided.

A Landscape Perspective On Bird Beak Deformity: An Epizootic Of Unknown Etiology, Rachelle Mclaughlin, Shaily Menon

Student Summer Scholars Manuscripts

Although birds with beak deformities have been documented throughout the literature, the recent spike in occurrences in certain regions has caused concern in the scientific community. A major concern relates to the role of contaminants and environmental degradation in causing or exacerbating this epizootic. This study used spatial and statistical analyses to examine the problem from a landscape perspective. The objectives of this study were to 1) locate and compile a database of known bird beak occurrences, 2) conduct a preliminary assessment of the environmental correlates of this epizootic in order to identify patterns, and 3) make recommendations that could …

Don’T Spray The Wasps! Using Polistes Paper Wasps For Pest Management In The Home Garden, Laura Goldsmith, Michael Henshaw

Student Summer Scholars Manuscripts

Pest control poses a challenge to all gardeners, especially those wishing to use organic practices. We examined the potential use of Polistes paper wasps as a bio-control agent in the organic backyard garden. Polistes are important predatory insects which may potentially consume thousands of soft bodied pest insects per season. We planted 8 garden plots with leaf lettuce, cabbage, pole beans and tomatoes. In 4 experimental plots, we established 8 Polistes dominulus nests, while in the remaining control plots we did not establish wasp nests. We measured the abundance of pest insects, the extent of damage from insect pests, and …

Population Biology Of A Coastal Dune-Dwelling Spider (Arctosa Sanctaerosae) Along The Hurricane Disturbed Northern Gulf Of Mexico Coast, Robert A. Hataway

Electronic Theses and Dissertations

With the continued increase in the number of tourists visiting the Northern Gulf coast in the last century and the resulting development of this coastline the habitat of Arctosa sanctaerosae has become fragmented; and the sprawl of large cities along the coast has further degraded available habitat. In addition to anthropogenic disturbance to this coastal region, hurricanes are an additional and natural perturbation to the ecosystem. This habitat has seen a number of major tropical storms over the last decade and I have sought to explore the impact of habitat destruction and storm-induced disturbance on a species of spider endemic …

Mechanical Forces And Tumor Cells: Insight Into The Biophysical Aspects Of Cancer Progression, Indrajyoti Indra

Wayne State University Dissertations

Mechanical forces play an important role in the regulation of cellular behavior and physiological processes including adhesion, migration, proliferation, tissue repair, embryogenesis and development. In addition, a number of diseases including cancer, have been linked to changes in cellular and extracellular mechanical properties. However, whether a correlation exists between the progression of cancer towards metastasis and mechanical factors has not been clearly defined. Additionally, how a cell responds to changes in extracellular mechanical cues as it gains metastatic abilities is poorly understood. To address these questions, we have utilized a panel of murine breast cancer cell lines with progressive metastatic. …

Rox1 Function In Dosage Compensation: Structural / Functional Analysis Of A Non-Coding Rna, Ying Kong

Wayne State University Dissertations

roX1 is a long non-coding RNA involved in the chromosome-wide gene regulation that occurs during dosage compensation in Drosophila. Dosage compensation in Drosophila melanogaster occurs by a global two-fold increase of transcription from the single male X chromosome. This essential process compensates for X chromosome monosomy. The male-specific lethal (MSL) complex, containing five proteins, localizes to the male X chromosome and alters chromatin to modify gene expression. roX1 and roX2 RNAs are redundant components of MSL complex that are required for its exclusive X-localization. Recent studies in our lab have revealed a second role of roX RNAs in heterochromatic gene …

Attachment Of Non-Cognate Chromophores To Cpca Of Synechocystis Sp. Strain Pcc 6803 Expressed In E. Coli, Wendy M. Schluchter

Biological Sciences Faculty Publications

No abstract provided.

Biosynthesis Of Cyanobacterial Phycobiliproteins In Escherichia Coli: Chromophorylation Efficiency And Specificity Of All Bilin Lyases From Synechococcus Sp. Strain Pcc 7002, Wendy M. Schluchter

Biological Sciences Faculty Publications

No abstract provided.

Effects Of Modified Phycobilin Biosynthesis In The Cyanobacterium Synechococcus Sp. Strain Pcc 7002, Wendy M. Schluchter

Biological Sciences Faculty Publications

No abstract provided.

Oxygen Limitation And Tissue Metabolic Potential Of The African Fish Barbus Neumayeri: Roles Of Native Habitat And Acclimatization, Martinez M. L, Raynard E. L, Bernard B. Rees, Chapman L. J

Biological Sciences Faculty Publications

Background: Oxygen availability in aquatic habitats is a major environmental factor influencing the ecology, behaviour, and physiology of fishes. This study evaluates the contribution of source population and hypoxic acclimatization of the African fish, Barbus neumayeri, in determining growth and tissue metabolic enzyme activities. Individuals were collected from two sites differing dramatically in concentration of dissolved oxygen (DO), Rwembaita Swamp (annual average DO 1.35 mgO2 L-1) and Inlet Stream West (annual average DO 5.58 mgO2 L-1) in Kibale National Park, Uganda, and reciprocally transplanted using a cage experiment in the field, allowing us to maintain individuals under natural conditions of …

Geographic Variation In The Effects Of Heat Exposure On Maximum Sprint Speed And Hsp70 Abundance In Populations Of The Western Fence Lizard, Scelopolus Occidentalis, D M. Mcmillan, D J. Irschick, Bernard B. Rees

Biological Sciences Faculty Publications

We examined whether western fence lizards Sceloporus occidentalis

occurring in thermally divergent environments display differential

responses to high temperature in locomotor performance

and heat-shock protein (Hsp) expression. We measured

maximum sprint speed in S. occidentalis from four populations

at paired latitudes and elevations before and after exposure to

an experimental heat treatment and then quantified hind-limb

muscle Hsp70 expression. Lizards collected from northern or

high-elevation collection sites suffered a greater reduction in

sprint speed after heat exposure than lizards collected from

southern or low-elevation sites. In addition, lizards from northern

collection sites also exhibited an increase in Hsp70 expression

after …

Effect Of Uv-A Irradiance On Lipid Accumulation In Nannochloropsis Oculata, Rathy Srinivas

Electronic Theses and Dissertations

The marine microalgae Nannochloropsis spp. are of both ecological and economic importance as they contribute to the base of the food chain of marine systems, and can accumulate a considerable amount of lipids. Their lipids can be grouped into two categories, storage lipids, which are mainly triglycerides (TGs), and structural lipids, which are composed of polyunsaturated fatty acids (PUFAs). PUFAs are essential nutrients for both aquatic animals and humans, while TGs can be transesterified to produce biodiesel. Lipid formation in microalgae depends on several environmental factors and Nannochloropsis spp. have been shown to accumulate excess storage lipids under stressful conditions. …

Indirect Estimates Of Gene Flow And Its Conservation Implications In The Striped Newt (Notophthalmus Perstriatus), Sarah Elizabeth May

Electronic Theses and Dissertations

This study used indirect methods to estimate patterns of gene flow in a rare salamander species, the striped newt (Notophthalmus perstriatus). First, we used combined genetic and ecological methods to determine whether populations that appear to exist in two regions separated by 125 km, exhibited genetic and ecological distinctness such that the regions demarcate separate conservation units. Using mtDNA (cyt-b), we found that haplotypes were shared between localities within each region but none were shared between regions. Niche-based distribution modeling revealed significant differences in the ecological setting between the two regions. In combination, the absence of evidence for recent genetic …