Biology Commons^™

Parity-Induced Decrease In Systemic Growth Hormone Alters Mammary Gland Signaling: A Potential Role In Pregnancy Protection From Breast Cancer, Robert K. Dearth, David A. Delgado, Jill K. Hiney, Thushangi Pathiraja, Steffi Oesterreich, Dan Medina, W. Les Dees, Adrian V. Lee

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Early full-term pregnancy is an effective natural protection against breast cancer in both humans and experimental rodents. The protective effect of an early pregnancy is in part linked to changes in circulating hormones that are involved in both normal breast development and breast cancer. For example, a reduction in circulating growth hormone (GH) has been shown to protect rats from carcinogen-induced mammary tumors. We examined the ability of a full-term pregnancy to alter the endocrine GH/IGF-I axis and how this change affected normal mammary gland function in two commonly used rat models (Sprague-Dawley and Wistar-Furth). Circulating GH and IGF-I were …

The Mayfly Newsletter, Peter M. Grant

The Mayfly Newsletter

The Mayfly Newsletter is the official newsletter of the Permanent Committee of the International Conferences on Ephemeroptera.

The Role Of Adaptive Trans-Generational Plasticity In Biological Invasions Of Plants, Andrew R. Dyer, Cynthia S. Brown, Erin K. Espeland, John K. Mckay, Harald Meimberg, Kevin J. Rice

Faculty Publications

High-impact biological invasions often involve establishment and spread in disturbed, high-resource patches followed by establishment and spread in biotically or abiotically stressful areas. Evolutionary change may be required for the second phase of invasion (establishment and spread in stressful areas) to occur. When species have low genetic diversity and short selection history, within-generation phenotypic plasticity is often cited as the mechanism through which spread across multiple habitat types can occur. We show that trans-generational plasticity (TGP) can result in pre-adapted progeny that exhibit traits associated with increased fitness both in high-resource patches and in stressful conditions. In the invasive sedge, …

Organismal Climatology: Analyzing Environmental Variability At Scales Relevant To Physiological Stress, Brian Helmuth, Bernardo R. Broitman, Lauren Yamane, Sarah E. Gilman, Katharine Mach, K. A.S. Mislan, Mark W. Denny

Faculty Publications

Predicting when, where and with what magnitude climate change is likely to affect the fitness, abundance and distribution of organisms and the functioning of ecosystems has emerged as a high priority for scientists and resource managers. However, even in cases where we have detailed knowledge of current species’ range boundaries, we often do not understand what, if any, aspects of weather and climate act to set these limits. This shortcoming significantly curtails our capacity to predict potential future range shifts in response to climate change, especially since the factors that set range boundaries under those novel conditions may be different …

Most, But Not All, Yeast Strains In The Deletion Library Contain The [Pin+] Prion, Anita L. Manogaran, Viviana M. Fajardo, Robert J. D. Reid, Rodney Rothstein, Susan W. Liebman

Biological Sciences Faculty Research and Publications

The yeast deletion library is a collection of over 5100 single gene deletions that has been widely used by the yeast community. The presence of a non-Mendelian element, such as a prion, within this library could affect the outcome of many large-scale genomic studies. We previously showed that the deletion library parent strain contained the [PIN⁺] prion. [PIN⁺] is the misfolded infectious prion form of the Rnq1 protein that displays distinct fluorescent foci in the presence of RNQ1–GFP and exists in different physical conformations, called variants. Here, we show that over 97% of the …

Foraging Rates Of Larval Dragonfly Colonists Are Positively Related To Habitat Isolation: Results From A Landscape-Level Experiment, Shannon J. Mccauley, Tomas Brodin, John Hammond

Biological Sciences

There is increasing evidence of intraspecific variation in dispersal behavior. Individual differences in dispersal behavior may be correlated with other traits that determine the impact individuals have on patches they colonize. We established habitat patches—artificial pools—across a landscape, and these pools were naturally colonized by dragonfly larvae. Larvae were collected from pools at different levels of isolation and held under common lab conditions for 5 months. We then compared larval foraging rates. Foraging rate was positively related to habitat isolation, and colonists from the most isolated artificial pools had significantly higher foraging rates than individuals from the least isolated pools. …

Temperature Affects Adhesion Of The Acorn Barnacle (Balanus Amphitrite), Laurel A. Johnston

Master's Theses

Biofouling is the accumulation of sessile marine organisms, such as algae, tube worms and barnacles on man-made substrata and has negative economic and ecological implications. Ship hulls are readily fouled, which significantly increases drag while decreasing ship fuel efficiency when moving through water. Fouled hulls have also become important vectors of invasive species. These problems are minimized when hulls are painted with a toxic anti-fouling or non-toxic foul-release coating. Due to recent restrictions of anti-fouling paint use, research and development of non-toxic alternatives has increased.

Novel hull coating efficiency is often quantified by the critical removal stress value of barnacles …

An Exonic Splicing Enhancer Within A Bidirectional Coding Sequence Regulates Alternative Splicing Of An Antisense Mrna, Valerie K. Salato, Nathaniel W. Rediske, Chao Zhang, Michelle Laura Hastings, Stephen Munroe

Biological Sciences Faculty Research and Publications

The discovery of increasing numbers of genes with overlapping sequences highlights the problem of expression in the context of constraining regulatory elements from more than one gene. This study identifies regulatory sequences encompassed within two genes that overlap in an antisense orientation at their 3’ ends. The genes encode the α-thyroid hormone receptor gene (TRα or NR1A1) and Rev-erbα (NR1D1). In mammals TRα pre-mRNAs are alternatively spliced to yield mRNAs encoding functionally antagonistic proteins: TRα1, an authentic thyroid hormone receptor; and TRα2, a non-hormone-binding variant that acts as a repressor. TRα2-specific splicing requires two regulatory elements that overlap with Rev-erbα …

Are Beaches' Suitability As Sand Crab Habitat Affected By Human Recreation?, Jessica H. Murph, Zen Faulkes

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

METHODS We compared the populations of sand crabs (Lepidopa benedicti, family Albuneidae) at two locations on the popular tourist destination South Padre Island: one which is reasonably developed, having many hotels and restaurants and one which is not so developed, at a more northern point on the island. At each site, 10 m transects were dug starting at the top of the swash zone (parallel to the surf). Each shovel of sand were carefully sifted. The carapace length, sex, color, and reproductive status of each sand crab found were recorded on site. Samples were collected regularly from August 2009 to …

Molecular Cloning And Characterization Of Cdnas Encoding Metalloproteinases From Snake Venom Glands, Ying Jia, John C. Perez

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Snake venom metalloproteinases (SVMPs) are a superfamily of zinc-dependent proteases and participate in a number of important biological, physiological and pathophysiological processes. In this work, we simultaneously amplified 9 cDNAs encoding different classes of metalloproteinases from glands of four different snake species (Agkistrodon contortrix laticinctus, Crotalus atrox, Crotalus viridis viridis and Agkistrodon piscivorus leucostoma) by RT-PCR with a pair of primers. Among the encoded metalloproteinases, two enzymes (AclVMP-I and AplVMP-I), three enzymes (CaVMP-II, CvvVMP-II and AplVMP-II) and four enzymes (AclVMP-III, CaVMP-III, CvvVMP-III and AplVMP-III) …

Variation In The Heat Shock Response And Its Implication For Predicting The Effect Of Global Climate Change On Species' Biogeographical Distribution Ranges And Metabolic Costs, Lars Tomanek

Biological Sciences

The preferential synthesis of heat shock proteins (Hsps) in response to thermal stress [the heat shock response (HSR)] has been shown to vary in species that occupy different thermal environments. A survey of case studies of aquatic (mostly marine) organisms occupying stable thermal environments at all latitudes, from polar to tropical, shows that they do not in general respond to heat stress with an inducible HSR. Organisms that occupy highly variable thermal environments (variations up to >20°C), like the intertidal zone, induce the HSR frequently and within the range of body temperatures they normally experience, suggesting that the response is …

Positive Carotenoid Balance Correlates With Greater Reproductive Performance In A Wild Bird, Rebecca Safran, Kevin J. Mcgraw, Matt Wilkins, Joanna K. Hubbard, Julie Marling

School of Biological Sciences: Faculty Publications

Background: Carotenoids can confer somatic and reproductive benefits, but most evidence is from captive animal experimentation or single time-point sampling. Another perhaps more informative means by which to assess physiological contributions to animal performance is by tracking an individual’s ability to increase or sustain carotenoids or other health-related molecules over time, as these are likely to be temporally variable.

Methodology/Principal Findings: In a field study of North American barn swallows (Hirundo rustica erythrogaster), we analyzed within-individual changes in carotenoid concentrations by repeatedly sampling the carotenoid profiles of individuals over the course of the breeding season. Our results demonstrate …

Crop Updates 2010 - Genetically Modified Crops, Nutrition And Soils, James Fisher, Désirée Futures, Peter Tozer, Mike Jackson, John Moore, Jamees Neilsen, Geoff Anderson, Wen Chen, Richard Bell, Paul Blackwell, Allan Herbert, Stephen Davies, Ross Brennan, Mike Bolland, James Easton, Ryan Guthrie, Rowan Madderm, Robert Belford, Wal Anderson, Ian Edwards, Reg Lunt, Bill Bowden, Nigel Metz, Peter Newman, Breanne Best, Chris Gazey, Joel Andrew

Crop Updates

GENETICALLY MODIFIED CROPS

1. Evaluation of the environmental and economic impact of Roundup Ready® canola in the Western Australian crop production system, James Fisher and Désirée Futures, York, Western Australia, Peter Tozer, PRT Consulting, Armidale NSW

2. Controlling wild radish (Raphanus raphanistrum) in Roundup Ready®¹ Canola: Outcomes from the Nufarm 2009 Roundup Ready small plot trial Program, Mike Jackson, Nufarm Australia Limited

3. Weed strategies for glyphosate tolerant crops, John Moore, Department of Agriculture and Food

4. Results of the 2009 Western Australia Roundup Ready® canola trials, Dr James Neilsen, Canola Systems …

The Evolution Of Human Genetic And Phenotypic Variation In Africa, Michael Campbell, Sarah Tishkoff

Department of Biology Faculty Publications

Africa is the birthplace of modern humans, and is the source of the geographic expansion of ancestral populations into other regions of the world. Indigenous Africans are characterized by high levels of genetic diversity within and between populations. The pattern of genetic variation in these populations has been shaped by demographic events occurring over the last 200,000 years. The dramatic variation in climate, diet, and exposure to infectious disease across the continent has also resulted in novel genetic and phenotypic adaptations in extant Africans. This review summarizes some recent advances in our understanding of the demographic history and selective pressures …

Analysis Of Primary Risk Factors For Oral Cancer From Us States With Increasing Rates, Anthony Bunnell, Nathan Pettit, Nicole Reddout, Kanika Sharma, Susan O'Malley, Michelle Chino, Karl Kingsley

School of Public Health Faculty Research

Objectives

To examine the primary risk factor for oral cancer in the US, smoking and tobacco use, among the specific US states that experienced short-term increases in oral cancer incidence and mortality.

Methods

Population-based data on oral cancer morbidity and mortality in the US were obtained from the National Cancer Institute's (NCI) Surveillance, Epidemiology, and End Results (SEER) database for analysis of recent trends. Data were also obtained from the Centers for Disease Control and Prevention (CDC) Behavioral Risk Factor Surveillance System (BRFSS) to measure current and former trends of tobacco usage. To comprehensive measures of previous state tobacco use …

Biogeography Of The American Pika (Ochotona Princeps) In Oregon And Southern Washington: Illuminating Genetic Relationships Among Disjunct Populations, George Washington Batten Iii

Dissertations and Theses

The American pika (Ochotona princeps) finds moderately warm temperatures (>25°C) lethally stressful, and at the end of the last Ice Age 10,000 years ago was forced to disperse to cooler, "sky island" mountaintops where they are almost exclusively found today. Thirty six subspecies are recognized, all established using morphological characters, and it is uncertain whether these subspecies' designations are corroborated by genetic analyses. This study elucidates three hypotheses regarding populations in Oregon and southern Washington: 1) O. p. fumosa constitutes a subspecies distinct form O. p. brunnescens, 2) the Columbia River constitutes a barrier to gene …

Detection Of Wuchereria Bancrofti L3 Larvae In Mosquitoes: A Reverse Transcriptase Pcr Assay Evaluating Infection And Infectivity, Sandra J. Laney, Reda M.R. Ramzy, Hanan H. Helmy, Hoda A. Farid, Ameen A. Ashour, Gary J. Weil, Steven A. Williams

Biological Sciences: Faculty Publications

Lymphatic filariasis is a disabling and disfiguring disease caused by a parasite that is transmitted by a mosquito. The life cycle of the parasite requires two hosts: the mosquito vector and the human host. Part of the developmental life cycle of the parasite occurs in the mosquito and the other part in the human host. The parasite develops through four stages in the mosquito, only the last of which is infectious to humans. The third larval stage (L3) is the infective stage that initiates human infections when infective mosquitoes bite humans. There is currently a global program attempting to eliminate …

An Analysis Of Phenotypic Marker Presence On Umr-106 Cells Following Acclimation To Media Reduced In Estrogenic Compounds, Levi Castle

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by Levi Castle on February 12, 2010.

Energetic Basis Of Colonial Living In Social Insects, Chen Hou, Michael Kaspari, Hannah B. Vander Zanden, James F. Gillooly

Biological Sciences Faculty Research & Creative Works

Understanding the ecology and evolution of insect societies requires greater knowledge of how sociality affects the performance of whole colonies. Metabolic scaling theory, based largely on the body mass scaling of metabolic rate, has successfully predicted many aspects of the physiology and life history of individual (or unitary) organisms. Here we show, using a diverse set of social insect species, that this same theory predicts the size dependence of basic features of the physiology (i.e., metabolic rate, reproductive allocation) and life history (i.e., survival, growth, and reproduction) of whole colonies. The similarity in the size dependence of these features in …

Hormones And Reproductive Cycles In Snakes, Emily N. Taylor, Dale F. Denardo

Biological Sciences

The study of the hormonal regulation of reproduction in snakes is in its infancy. Studies have disproportionately examined temperate zone viperid and colubrid snakes, especially the red-sided garter snake (Thamnophis sirtalis parietalis). Indeed, extensive observational and experimental studies on T. s. parietalis form the basis for our understanding of the hormonal regulation of reproduction in snakes. This review focuses on seasonal hormone concentrations in snakes in relation to events in the reproductive cycle; the limited data available on hypothalamic hormones, gonadotropins (GTHs), hormone receptors, and binding globulins; the neuroendocrinology of reproduction; and the environmental, social, physiological, and embryonic aspects of …

A Meta-Analysis Of Resource Pulse-Consumer Interactions, Louie H. Yang, Kyle F. Edwards, Jarrett E. Byrnes, Justin Bastow, Amber N. Wright, Kenneth O. Spence

Biology Faculty Publications

Resource Pulses are infrequent, large-magnitude, and short-duration events of increased resource availability. They include a diverse set of extreme events in a wide range of ecosystems, but identifying general patterns among the diversity of pulsed resource phenomena in nature remains an important challenge. Here we present a meta-analysis of resource pulse-consumer interactions that addresses four key questions: (1) Which characteristics of pulsed resources best predict their effects on consumers? (2) Which characteristics of consumers best predict their responses to resource pulses? (3) How do the effects of resource Pulses differ in different ecosystems? (4) What are the indirect effects of …

Evolution Of Terrestrial Habitat In Manophylax Species (Trichoptera:Apataniidae), With A New Species From Alaska, Suvtsetseg Chuluumbat, John C. Morse, Joanna L. Lessard, M Eric Benbow, Matthew D. Wesener, John Hudson

Publications

The genus Manophylax (Trichoptera:Apataniidae) contains 3 species from the Nearctic Region and 3 species from Japan. A 7th species of Manophylax new for science is described and illustrated from Alaska. Habitats of larvae and pupae of the different Manophylax species are either mostly hygropetric or mostly terrestrial. Phylogenetic analysis infers 3 principal species groups occurring in the eastern and western Nearctic and eastern Palearctic Biogeographic Regions. The habitat of the western Nearctic M. annulatus Species Group is mostly hygropetric and that of the eastern Nearctic M. altus and eastern Palearctic M. futabae Species Groups are mostly terrestrial. The phylogeny suggests …

Fishing Industry Impact Study : James Price Point Proposed Liquefied Natural Gas Precinct, Department Of Fisheries

Fisheries Occasional Publications

No abstract provided.

Latitudinal Differences In The Hibernation Characteristics Of Woodchucks (Marmota Monax), Stam M. Zervanos, Christine R. Maher Phd, Jerry A. Waldvogel, Gregory L. Florant

Faculty Publications

There is little information on the phenotypic flexibility of hibernation characteristics within species. To address this issue, we observed differences in hibernation characteristics of three free-ranging populations of woodchucks (Marmota monax) distributed along a latitudinal gradient from Maine to South Carolina. Data from free-ranging animals exhibited a direct relationship between latitude and length of the hibernation season. As expected, woodchucks in the northern latitudes hibernated longer than those in the southern latitudes. Also, the length of interbout arousals decreased with increase in latitude, whereas the length of torpor bouts and the number of arousals increased. Thus, we observed phenotypic plasticity …

Behavioral And Chemical Ecology Of Marine Organisms With Respect To Tetrodotoxin, Becky L. Williams

Biology Faculty Publications

The behavioral and chemical ecology of marine organisms that possess tetrodotoxin (TTX) has not been comprehensively reviewed in one work to date. The evidence for TTX as an antipredator defense, as venom, as a sex pheromone, and as an attractant for TTX-sequestering organisms is discussed. Little is known about the adaptive value of TTX in microbial producers; thus, I focus on what is known about metazoans that are purported to accumulate TTX through diet or symbioses. Much of what has been proposed is inferred based on the anatomical distribution of TTX. Direct empirical tests of these hypotheses are absent in …

The Presence Of Micropterus Salmoides (Largemouth Bass) Influences The Populations Of Rana Draytonii (California Red-Legged Frog) And Pseudacris Regilla (Pacific Treefrog) In Two Ponds In Santa Barbara County, California, Kenneth Lee Gilliland

Master's Theses

Alien fish have been implicated in the decline of Rana draytonii (California red-legged frog) and Pseudacris regilla (Pacific treefrog) populations. Micropterus salmoides (largemouth bass) is a common sport fish that has been introduced into the sympatric range of these two anuran species; however, no studies have investigated the individual influence that this alien fish has on these two species. I conducted M. salmoides control or eradication experiments during a four year study in two ponds within the Transverse Mountain Range of Santa Barbara County, California. Changes in the densities of all life stages of R. draytonii and P. regilla were …

Evolution & Phylogenetic Analysis: Classroom Activities For Investigating Molecular & Morphological Concepts, Wilfred A. Franklin

Biology Faculty Research and Scholarship

In a flexible multisession laboratory, students investigate concepts of phylogenetic analysis at both the molecular and the morphological level. Students finish by conducting their own analysis on a collection of skeletons representing the major phyla of vertebrates, a collection of primate skulls, or a collection of hominid skulls.

Genome Sequence Of The Pea Aphid Acyrthosiphon Pisum, International Aphid Genomics Consortium, Gregory K. Davis

Biology Faculty Research and Scholarship

Aphids are important agricultural pests and also biological models for studies of insect-plant interactions, symbiosis, virus vectoring, and the developmental causes of extreme phenotypic plasticity. Here we present the 464 Mb draft genome assembly of the pea aphid Acyrthosiphon pisum. This first published whole genome sequence of a basal hemimetabolous insect provides an outgroup to the multiple published genomes of holometabolous insects. Pea aphids are host-plant specialists, they can reproduce both sexually and asexually, and they have coevolved with an obligate bacterial symbiont. Here we highlight findings from whole genome analysis that may be related to these unusual biological …

Use Of Rpsl As A Counterselectable Marker In Borrelia Burgdorferi, Dan Drecktrah, Miles J. Douglas, D. Scott Samuels

Biological Sciences Faculty Publications

We have demonstrated that rpsL, encoding the S12 protein of the small ribosomal subunit, can be used as a counterselectable marker in Borrelia burgdorferi, the causative agent of Lyme disease. Mutations in rpsL confer streptomycin resistance. Streptomycin susceptibility is dominant in an rpsL merodiploid, and streptomycin selects for the loss of wild-type rpsL carried in trans. This is the first description of a counterselectable marker in B. burgdorferi.

Differences In The Transcriptome Signatures Of Two Genetically Related Entamoeba Histolytica Cell Lines Derived From The Same Isolate With Different Pathogenic Properties, Laura Biller, Paul H. Davis, Manuela Tillack, Jenny Matthiesen, Hannelore Lotter, Samuel L. Stanley, Egbert Tannich, Iris Bruchhaus

Biology Faculty Publications

Results: The availability of two genetically very similar cell lines (A and B) derived from the laboratory isolate Entamoeba histolytica HM-1:IMSS, which differ in their virulence properties, provides a powerful tool for identifying pathogenicity factors of the causative agent of human amoebiasis. Cell line A is incapable inducing liver abscesses in gerbils, whereas interaction with cell line B leads to considerable abscess formation. Phenotypic characterization of both cell lines revealed that trophozoites from the pathogenic cell line B have a larger cell size, an increased growth rate in vitro, an increased cysteine peptidase activity and higher resistance to nitric …