Biology Commons^™

Chemical Genetics Of Plasmodium Falciparum, W. Armand Guiguemde, Agnang A. Shelat, David Bouck, Sandara Duffy, Gregory J. Crowther, Paul H. Davis, David S. Smithson

Biology Faculty Publications

Malaria caused by Plasmodium falciparum is a disease that is responsible for 880,000 deaths per year worldwide. Vaccine development has proved difficult and resistance has emerged for most antimalarial drugs. To discover new antimalarial chemotypes, we have used a phenotypic forward chemical genetic approach to assay 309,474 chemicals. Here we disclose structures and biological activity of the entire library—many of which showed potent in vitro activity against drug-resistant P. falciparum strains—and detailed profiling of 172 representative candidates. A reverse chemical genetic study identified 19 new inhibitors of 4 validated drug targets and 15 novel binders among 61 malarial proteins. Phylochemogenetic …

Production And In Vitro Investigation Of Vitamin B12-Based Bioprobes, Amy Rabideau

Renée Crown University Honors Thesis Projects - All

Vitamin B₁₂ (B₁₂)-based bioprobes were produced and investigated in vitro for studying the B₁₂ uptake pathway in certain cancer cell lines and their target-specific capabilities as imaging and/or contrast agents in vivo. An intrinsic factor (IF)-bound B₁₂ conjugate (B₁₂-ReBQBA) was produced containing rhenium(I) for in vitro analysis of the cubilin-expressing placental choriocarcinoma cell line. B₁₂-ReBQBA has millimolar toxicity in vitro and excitation and emission wavelengths in the visible region, 488 nm and 560 nm, respectively. The rhenium metal in this bioprobe can be readily exchanged for the metastable radioactive metal technetium-99m …

Acyl Homoserine Lactone Signaling In Pseudomonas Chlororaphis 06, Anna Hegsted

Undergraduate Honors Capstone Projects

Pseudomonas chlororaphis (PcO6), a rhizosphere bacterium, is important to agriculture because of its use as a biological control against fungus and other pests. This biological control correlates with production of phenazines; a process regulated by acyl homoserine lactones (AHSLs). Long chain AHSLs might also have an affect on the production of phenazines. My research showed that PcO6 produced long chain AHSLs and that production is affected by nutrition with more long chain AHSLs produced in rich medium than minimal medium. The nutritional studies and findings with different mutants of PcO6 altered in phenazine production suggest that control of these antifungal …

Coyote Diet And Movements In Relation To Winter Recreation In Northwestern Wyoming: Implications For Lynx Conservation, Jennifer L. Burghardt Dowd

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Increased snowmobile use in mountainous terrain has been highlighted as a conservation concern for some Canada lynx (Lynx canadensis) populations. Snow compaction resulting from winter recreation may potentially facilitate access by coyotes (Canis latrans) to habitats used by lynx during winter. Increased interactions could result in either exploitation or interference competition between the two species. Two recent, yet geographically distinct, studies showed contrasting findings regarding coyote movements and their use of snow-compacted trails during the winter. These findings suggest coyote association with snow-compacted trails may be regionally specific and dependent upon ecosystem dynamics and snow characteristics. …

Survival Of Mycoplasma Species In Recycled Bedding Sand And Possible Implications For Disease Transmission To Ruminants, Anne E. Justice-Allen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Mycoplasmas are a group of bacteria which are small in size, lack a cell wall, and have small genomes in comparison to other bacteria. The members of the group that are pathogenic utilize several mechanisms to evade the host's immune system. These processes affect surveillance and control mechanisms such as serologic testing and vaccination. Many of these organisms cause diseases of livestock, which heavily impact production parameters such as weight gain, milk yield, and egg production. Mycoplasmas also cause disease in people.

Mycoplasma spp. can cause mastitis, metritis, pneumonia, and arthritis. The currently documented routes of transmission of Mycoplasma spp. …

Initial Analysis O F The 3' Spliced Region Of The Receptor Tyrosine Kinase Ret, Ananya Sengupta

Theses, Dissertations and Culminating Projects

The RET proto-oncogene is expressed in the developing kidney and enteric nervous system during vertebrate embryogenesis. It has two major splicing variants; RET9 and RET51. The expression pattern of the two variants appears to vary among the developing organisms. RET9 plays a major role in development of kidney and excretory system whereas the role of RET 51 seems to be of maintenance of these systems after development. The protein RET is a transmembrane receptor which expresses itself in the cell surface and initiates the signal transduction pathways for cellular differentiation and proliferation of the cell. The different expression patterns shown …

Identification Of Yeasts From The Gut Of Cotinis Nitida Using Its And Lsu Rrna D1/D2 Gene Sequences, Kevin Campbell

Honors Capstones

No abstract provided.

The Cytotoxicity Of Nitrite And Nitrate In E. Coli, Aaron P. Landry

Honors Capstones

No abstract provided.

The Effects Of Triptolide On Cancer Cells, Michael E. Messina Jr.

Theses, Dissertations and Culminating Projects

Cancer is the second leading cause of death in the United States, and with the rates of breast cancer increasing steadily over time there is a need to develop new anticancer treatments. This research seeks to investigate how triptolide, an extract from the Chinese herb Tripterygium wilfordii Hook F, induces apoptosis in human breast cancer cells. Studies have shown that triptolide can act as an anti-proliferative, antiinflammatory, and anti-cancer agent in many different clinical applications. It has been used for over two centuries in Chinese medicine and is now used to treat certain autoimmune diseases, such as rheumatoid arthritis. With …

Nested Subsets, Scale, And The Distribution Of Abundance: A Macroecological Approach, Elita Baldridge

Master's Theses or Doctor of Nursing Practice

While the canonical nested subset pattern suggests that less species-rich areas will contain a proper subset of the species observed in richer areas, actual data sets do not show perfect nestedness; however, they show a pattern with more structure than would be expected by chance. Biological processes like immigration and extinction have traditionally been thought to produce the nested subset pattern. These processes acting indirectly could cause variation in the distribution and abundance of species that could produce nestedness at a variety of scales. Determining at what scales the pattern is observed might allow inference of processes that are more …

Herpetofaunal Response To Prescribed Burning On Matagorda Island, Texas: With Emphasis On Texas Horned Lizard (Phrynosoma Cornutum), Ashley Inslee

Master's Theses or Doctor of Nursing Practice

Texas horned lizard (Phrynosoma cornutum) populations have been in steady decline over the past twenty years due to habitat loss, pesticide use, the pet trade, and invasion of the red imported fire ant (Solenopsis invicta). At Aransas National Wildlife Refuge the P. cornutum population has declined, except for on Matagorda Island. A prescribed burn regime was initiated to maintain the coastal prairie habitat. This closed environment was unique in its composition of arid adapted and wetland species. There was minimal human disturbance, and a single road bisects the length of the island. The island was invaded by S. invicta around …

Climate Change: Implications For Montane Mammals Of The Great Basin, Georgina Yvette Jacquez

Master's Theses or Doctor of Nursing Practice

Climate change threatens biodiversity; in particular, species with narrow distributions and specific habitat requirements. The Great Basin provides an excellent model system to evaluate the effects of climate change on species with isolated distributions and specific habitat requirements. I have evaluated the McDonald and Brown (1992) model that examined the effects of climate change on montane mammals of the Great Basin based on its underlying assumptions and model predictions. I have modeled the distributions of twelve montane mammal species found in the Great Basin and identified potential local extinctions by using maximum entropy modeling (Maxent) for two emission scenarios of …

Flooding Tolerance Of Native And Nonnative Grasses: Variation In Photosynthesis, Transpiration, Respiration, And Carbon Isotope Discrimination, Elizabeth Waring

Master's Theses or Doctor of Nursing Practice

Invasion by nonnative plants is particularly prevalent in wetlands. While the ecological patterns in wetland plants are well known, it is less well known how flooding-related soil conditions influence the physiological success of introduced species in wetlands. In chapter 1, effects of flooding were measured in invasive common reed (Phragmites australis), reed canary grass (Phalaris arundinacea), Johnson grass (Sorghum halepense), and native prairie cordgrass (Spartina pectinata). The four species were kept at four levels of flooding (deep flooding, medium flooding, low flooding, and dry conditions), and their responses were measured after 7 and 28 days of treatment using by a …

Variation In The Southern Short-Tailed Shrew, Blarina Carolensis, Jennifer O'Neill

Master's Theses or Doctor of Nursing Practice

The southern short-tailed shrew (Blarina carolinensis) inhabits the Mississippi Alluvial Plain, the Gulf Coastal Plain, Florida, and the Atlantic Coastal Plain as far north as Virginia. Since its description by Bachman in 1837, this species has been widely studied and has a long and convoluted taxonomic history. Many of the morphometric studies were performed in different geographic locations and used different methodologies. These differences have made it difficult to make broad statements about the relationships within this species. Recently, a study of cranial measurements of specimens from the three subspecies of B. carolinensis found in Florida, identified a small population …

The Molecular Analysis Of Hemoparasite Burdens Of Free-Ranging Lions (Panthera Leo) In The Kruger National Park, South Africa, Jamie R. Sherman

Renée Crown University Honors Thesis Projects - All

Hemoparasites, or parasites of the blood transmitted by arthropod vectors, are commonly found in domestic animals and wildlife all over the world. Although hemoparasites can cause serious illness in domestic species, they often persist at low levels in wildlife without compromising general health. Current reports on wildlife parasitemia, specifically in lions (Panthera leo) are obtained from zoological parks and managed game reserves. However, few studies have examined parasitic burdens of free ranging lion populations. This study aims to semi quantitatively evaluate the presence of four representative hemoparasites including Babesia sp., Theileria sp., Cytauxzoon sp., and …

Differential Gene Expression And Protein Abundance Evince Ontogenetic Bias Toward Castes In A Primitively Eusocial Wasp, James H. Hunt, Florian Wolschin, Michael T. Henshaw, Thomas C. Newman, Amy L. Toth, Gro V. Amdam

Peer Reviewed Publications

Polistes paper wasps are models for understanding conditions that may have characterized the origin of worker and queen castes and, therefore, the origin of paper wasp sociality. Polistes is ‘‘primitively eusocial’’ by virtue of having contextdependent caste determination and no morphological differences between castes. Even so, Polistes colonies have a temporal pattern in which most female larvae reared by the foundress become workers, and most reared by workers become future-reproductive gynes. This pattern is hypothesized to reflect development onto two pathways, which may utilize mechanisms that regulate diapause in other insects. Using expressed sequence tags (ESTs) for Polistes metricus we …

Testing Hypotheses For Exotic Plant Success: Parallel Experiments In The Native And Introduced Ranges, Jennifer L. Williams, Harald Auge, John L. Maron

Biological Sciences Faculty Publications

A central question in ecology concerns how some exotic plants that occur at low densities in their native range are able to attain much higher densities where they are introduced. This question has remained unresolved in part due to a lack of experiments that assess factors that affect the population growth or abundance of plants in both ranges. We tested two hypotheses for exotic plant success: escape from specialist insect herbivores and a greater response to disturbance in the introduced range. Within three introduced populations in Montana, USA, and three native populations in Germany, we experimentally manipulated insect herbivore pressure …

Spatial And Temporal Patterns Of Bald Eagle (Haliaeetus Leucocephalus) Winter Habitat Use Along The Upper Mississippi River Corridor, Ryan T. Schmitz

Master's Theses or Doctor of Nursing Practice

From March 1999 through February 2006, satellite transmitters were placed on 13 wild-caught individuals of the Bald Eagle (Haliaeetus leucocephalus) in southwestern Wisconsin. These individuals (6 males, 7 females, 11 adults, 2 immatures) were tracked along the Upper Mississippi River corridor during the winters of 1999 through 2006. The objectives of my study were to: 1) estimate winter home range size and to compare home range size between sexes, 2) characterize and quantify winter night roost habitat, and 3) identify spatial and temporal patterns of winter habitat use and behavior relative to temperature, wind speed, and ice-cover. Mean 95% fixed …

A Molecular Basis For Craniofacial Laterality In Fishes, Karen Adams

Renée Crown University Honors Thesis Projects - All

Laterality, the preference for development on one side of the body, is essential to the vertebrate body plan. While the vertebrate skeleton usually develops symmetrically, the processes underlying craniofacial laterality are not well understood. Using zebrafish and cichlids as model organisms, this study focuses on the molecular basis of symmetric craniofacial development. Fgf8, Retinoic Acid, and wnt11 were examined to analyze their involvement in regulating craniofacial laterality. Fgf8 is known to play a role in proper jaw development. Because fgf8 and Retinoic Acid interact to achieve laterality of the somites during somitogenesis, the role of Retinoic Acid in regulating …

Bioengineering Tissue Constructs Using Elastic Alginate Hydrogels, Barbana A. Graham

Biological Sciences

Bioengineered 3-D tissue constructs have great potential for understanding tissue development and tissue repair in patients lacking functional organs. One of the major challenges faced in the field, however, is to build functional tissue constructs that resemble tissue found in vivo. Cells and tissues in the body are organized into three-dimensional architectures, which interact with fibrillar extracellular matrix (ECM) proteins at a nanoscale. Both the topology and elasticity of the ECM play critical roles in regulating tissue formation. Alginate, a naturally occurring polysaccharide, is a good candidate to use as a biomaterial to mimic the topography and elasticity of the …

In Vitro Analysis Of Microfluidic Neural Probes, Joshua Kessler

Biological Sciences

The clinical impact of neural prostheses has been inhibited by a lack of functional stability of the devices due to acute and chronic reactive tissues' responses in the brain. However, prosthetic stability could be improved via local drug administration or delivery of siRNA to mitigate the reactive tissues using microfluidic probes. Neural prosthetic probes have been previously developed, but had asymmetric and uneven distribution of fluid delivery to brain phantoms. Therefore, probes with different geometries and fluid delivery pores (holes ranging from 10 to 20 μm) were tested for functionality in vitro using a crystal violet/PBS solution. Compound light microscopy …

High-Throughput Screening Studies Of Inhibition Of Human Carbonic Anhydrase Ii And Bacterial Flagella Antimicrobial Activity, Albert A. Barrese Iii

Dissertations

Complete abstract attached as supplemental file.

Effect Of Inflorescence Size On Female Choice In Wild Radish, Raphanus Raphanistrum, Mylenne H. Salinas

Renée Crown University Honors Thesis Projects - All

Sexual selection is an important mechanism of speciation. Originally studied in animals, researchers now argue that plants also exhibit characteristics that have been altered by sexual selection. Flower size, nectar production, pollen load, flower scent, and petal color are plant traits that participate in attracting mates through an intermediate pollinator. Although female choice has been demonstrated to enhance offspring quality in animals, a thorough analysis remains to be conducted in plants. In this study, I experimentally altered female choice by manipulating flower number on the hermaphroditic plant, Raphanus raphanistrum. Plants with smaller floral displays had reduced female choice; thus, allowing …

Role Of Estrogen Receptor Expression In Estrogen (E-2)-Induced Modulation Of Th1 And Th2 Cytokine Responses, Bertille Gaigbe-Togbe

Renée Crown University Honors Thesis Projects - All

Previous studies have demonstrated that repeated administration of 17β-estradiol (E-2) at a dose of 1mg/kg to both (SWR x NZB)F₁ (SNF₁) mice and (DBA x BALB/c)F1(DBF₁) mice resulted in significantly decreased survival and increased appearance of lupus nephritis pathology. However, the ways in which estrogen modulates immune responses has yet to be fully understood, specifically in regard to modulations in T_H1 and T_H2 cytokine profile. Various studies have reported the presence of two estrogen receptors (ER), a and b, which have been found to be constitutively expressed in many immune cells. …

Observation Of Growth Characteristics And Identification Of Mercuric Resistance Genes In Synechococcus Elongatus Sp. 625, Chiedozie Okafor

Theses, Dissertations and Culminating Projects

Due to high levels of heavy metal pollution in the environment, there has always been a high interest in organisms that have developed resistance to certain heavy metals of interest. Microorganisms such as Pseudomonas, E. coli, and Ralstonia, have been identified as being resistant to one or more heavy metals. Extensive work has been done with respect to mechanisms of resistance to heavy metals in a wide array of microorganisms. However, mechanisms of resistance are yet to be fully explored in some other microorganisms. One such example is the Cyanobacteria, Synechococcus sp. 625 (SIU 625), formerly classified under Anastycis nidulans. …

Analysis Of Potential Mutagenic Effects Of World Trade Center Dust On In Vitro Systems, Barbara Domingos Soares

Theses, Dissertations and Culminating Projects

Ever since the 1950s, air pollution has been linked to decreased lung function and increased cancer risk. Air pollution can consist of natural or artificial materials that range in chemical as well as physical properties. Inhaled pollutants can have a variety of effects on organisms ranging from minor irritation, to chronic obstructive pulmonary disease, and even cancer or death. Historically, it has been noted that increased levels of air pollution are associated with decreases in human health and increases in mortality.

Following the World Trade Center tragedy, rescue workers as well as New York City residents were exposed to thousands …

Conservation And Ecology Of The Yellow-Blotched Sawback (Graptemys Flavmaculata), William Winsyl Selman Ii

Dissertations

The role of species conservation is becoming increasingly important due to the mounting pressures from humans on habitats and populations of organisms. This is particularly evident in riverine ecosystems throughout the world where the human demand for freshwater resources is increasing, and consequently, the number of imperiled aquatic organisms is also growing. The seven chapters of this dissertation primarily focus on the Yellow-blotched Sawback, Graptemys flavimaculata, an endangered riverine turtle that is endemic to the Pascagoula River system of southeast Mississippi, USA. At the population level, we aimed to study the impacts of Hurricane Katrina on two study populations within …

Determining The Relationship Of Human Enteric Viruses In Clinical, Wastewater, And Environmental Samples Utilizing Molecular And Cell Culture Techniques, Jacquelina Susann Williams Woods

Dissertations

This study was the first to examine five significant enteric viruses in human fecal material, sewage, and oysters to show a genetic relationship between human enteric viruses and different sample matrices. Fecal samples were collected from an area hospital and examined for norovirus genotype I (NoV GI), norovirus genotype II (NoV Gil), hepatitis A virus (HA V), adenovirus (ADV), and enteroviruses. During this study, sewage samples were collected from a Waster Water Treatment Plant (WWTP) in Mobile, ALand oyster sentinels were placed at 0.1 nautical miles (nm) (station 1 ), 0.2nm (station 2}, 1.5nm (station 3), and 4nm (station 4) …

Formation Of Testosterone's 5alpha-Reduced, Versus Aromatized, Products Can Have Beneficial Cognitive And Anti-Anxiety Effects Without Negative Effects On Prostate Or Sexual Behavior Of Male Rats, Daniel Dacosta

Biological Sciences

Testosterone (T) can alter sexual, social, anxiety-like, and/or cognitive behavior of male rodents and exert trophic effects on the prostate. However, whether these effects are due to actions of T, or its 5-reduced and/or aromatized metabolites, is of interest. We tested the hypothesis that T’s effects to enhance prostate proliferation, sexual, social, cognitive and/or anti-anxiety-like behavior require formation of 5-reduced and/or aromatized metabolites. Gonadectomized (GDX) or gonadally-intact rats were administered T-containing, or empty, silastic capsules in conjunction with a 5-reductase inhibitor (finasteride; Experiment 1) or an aromatase inhibitor (formestane; Experiment 2). The performance of rats in sexual, cognitive (object recognition, …

Activity-Dependent Sharpening Of The Retinotectal Map In Zebrafish: The Role Of The Par Complex, Laura Mariconda

Biological Sciences

Visual activity acts via NMDA (N-methyl D-aspartate) receptors in order to refine developing retinotectal maps by shaping retinal arbors. In zebrafish larvae, each arbor forms synapses by adding and deleting many trial and error branches. Branches with synapses in retinotopic sites are selectively stabilized and new branches are added next to existing synapses, while distant branches are deleted. The resulting arbor is compact and has a bushy appearance. When NMDARs are blocked, arbors become larger and dynamic rates increase almost twofold, suggesting that the release of a stabilizing signal has been prevented. A likely suspect for this retrograde stabilizing signal …