Biology Commons^™

Prevalence And Intensity Of Trypanosome Infections In Stable And Declining Populations Of Brush-Tailed Bettongs (Bettongia Penicillata), Melissa Chiasson

Independent Study Project (ISP) Collection

The brush-tailed bettong (Bettongia penicillata), or woylie, is an endemic Australian marsupial that has recently suffered a large population crash in the Upper Warren region of Western Australia. Research has shown that both the declining Upper Warren population of brush-tailed bettongs and a stable population at Karakamia Wildlife Sanctuary are infected with a novel Trypanosoma sp., and the declining Upper Warren population was found to have a higher prevalence of trypanosome infection that that of the stable Karakamia population. In this study, further work was done to see if 1) the prevalence and intensity of trypanosome infection still differed significantly …

Mojave Applied Ecology Notes Fall 2009, Tom Monaco, Scott R. Abella, Amber Lee, James E. Deacon

Mojave Applied Ecology Notes

USDA working to manage invasive annual grasses, effects of heat and smoke on red brome soil seed bank, how burial depth and substrate affect germination of Sahara mustard and red brome, environmental effects of the southern Nevada groundwater project

Fisheries Occasional Publication No 75 - Research And Development Plan 2009-10, Department Of Fisheries Western Australia

Fisheries Occasional Publications

The Department of Fisheries R&D plan for 2009/10 outlines the research, monitoring and assessment activities that are specifically directed towards supporting the collection of information that will assist in achieving the objectives of the Fish Resources Management Act (1994) for the coming five year period. This series is updated on an annual basis and should be read in conjunction with the most recent State of Fisheries report where comprehensive analyses of the current status of each of the fisheries and fish habitat resources are described.

The plan specifically focuses on those activities that are currently planned or identified which directly …

First Records Of Hypleurochilus Geminatus And Centropristis Philadelphica From Chesapeake Bay, Aimee D. Halvorson

Virginia Journal of Science

During the fall of 2007, Centropris philadelphica (rock seabass) and Hypleurochilus geminatus (Crested blenny) were collected from Chesapeake Bay. These captures are significant as they represent the first substantiated record of C. philadelphica from Chesapeake Bay and only the second and third validated records of H. geminatus. Additionally, the first record of H. geminatus from Chesapeake Bay was only recently recognized since the specimen had been previously misidentified as Parablennius marmoreus (seaweed blenny). The collection of seven individuals of H. geminatus in 2007, from two locations, indicates that the species may be resident within the Chesapeake Bay estuary.

Phytoplankton Blooms: Their Occurrence And Composition Within Virginia's Tidal Tributaries, Harold G. Marshall, Todd A. Egerton

Virginia Journal of Science

Sporadic algal bloom development within a 10 year monitoring program in Virginia tidal tributaries of Chesapeake Bay is reviewed. These blooms were common events, characteristically producing a color signature to the surface water, typically short lived, occurring mainly from spring into autumn throughout different salinity regions of these rivers, and were produced primarily by dinoflagellates. The abundance threshold levels that would identify bloom status from a non-bloom presence were species specific, varied with the taxon's cell size, and ranged from ca. 10 to 10⁴ cells mL^-1. Among the most consistent sporadic bloom producers were the dinoflagellates Akashiwo …

Reducing The Impact Of Pcr-Mediated Recombination In Molecular Evolution And Environmental Studies Using A New-Generation High-Fidelity Dna Polymerase, Daniel J.G. Lahr, Laura A. Katz

Biological Sciences: Faculty Publications

PCR-mediated recombination can greatly impact estimates of diversity, both in environmental studies and in analyses of gene family evolution. Here we measure chimera (PCR-mediated recombinant) formation by analyzing a mixture of eight partial actin sequences isolated from the amoeba Arcella hemisphaerica amplified under a variety of conditions that mimic standard laboratory situations. We further compare a new-generation proofreading processivity-enhanced polymerase to both a standard proofreading enzyme and previously published results. Proofreading polymerases are preferred over other polymerases in instances where evolutionary inferences must be made. Our analyses reveal that reducing the initial template concentration is as critical as reducing the …

Mangroves And Crabs As Ecosystem Engineers In Zanzibar, Charlie Nicholson

Independent Study Project (ISP) Collection

Primary productivity within mangroves results from detrital and coprophagous interactions on the forest floor. The feeding behaviors of Sesarmid crabs (Decapoda:Brachyura) alter the structural and chemical composition of benthic sediment through the consumption and incorporation of mangrove leaf-litter. In doing so they create habitats for organisms that in turn provide an additional food source for herbivorous crabs. Species specific herbivory was observed in order to understand the implications of Sesarmid activity on sediment composition. The study was conducted at two contrasting mangrove forest sites, one in a protected area at Jozani and the other in a disturbed area at Pete …

Selective Filtration In The Tropical Marine Sponge Rhopaloeides Odorabile: Impacts Of Elevated Seawater Temperature On Feeding Behavior, Andrew Massaro

Independent Study Project (ISP) Collection

Climate change currently represents the most significant and increasing threat to coral reef ecosystems worldwide as sea surface temperatures are predicted to increase by up to 4oC by the year 2099. Sponges that rely on strong microbial symbioses are particularly sensitive to elevations in seawater temperature. In this study, the impacts of elevated seawater temperature on feeding behavior in the tropical marine sponge Rhopaloeides odorabile were assessed. Sponges were exposed to temperatures ranging between 27 and 32oC. At four time points, filtration rate and volume flow rate of each sponge were measured, and feeding efficiencies on both heterotrophic and phototrophic …

Controlling The Dengue Mosquito (Aedes Aegypti): Assessment Of The Effects Of Metofluthrin, A Novel Vapor-Active Pyrethroid, On Mosquito Behavior In A Modeled Domestic Setting., Randy Kring

Independent Study Project (ISP) Collection

Dengue fever is a potentially life-threatening illness that is endemic in over 100 countries and threatens approximately half of the world’s population. The dengue virus is transmitted between humans by a mosquito vector, principally the dengue mosquito, Aedes aegypti. This mosquito prefers to live and feed in and around people’s homes. As a result, preventing mosquito bites within a domestic setting is essential to controlling the dengue virus. Although several methods of bite prevention are currently available to the public, most of these methods only provide short-term protection from the dengue mosquito. In contrast, a recently developed synthetic pyrethroid called …

Differences In Leaf Traits, Leaf Internal Structure, And Spectral Reflectance Between Two Communities Of Lianas And Trees: Implications For Remote Sensing In Tropical Environments, G. Arturo Sanchez-Azofeifa, Karen Castro, S. Joseph Wright, John Gamon, Margaret Kalacska, Benoit Rivard, Stefan A. Schnitzer, Ji Lu Feng

Biological Sciences Faculty Research and Publications

Increasing dominance of lianas in many tropical forests is considered a fingerprint of global environmental change. Despite the key role they play in ecosystem functioning, lianas remain one of the least studied life forms in tropical environments. This paper contrasts leaf traits and spectral properties (400–1100 nm) of liana and tree communities from a tropical dry forest and a tropical rainforest in Panama, Central America. Differences between lianas and tree leaf traits were analyzed using spectroscopy, leaf histology and pigment extractions. Results from this study indicate that many of the biochemical, structural, and optical properties of lianas …

Maternally Derived Antibodies In Avian Eggs And Offspring: Ecology, Life History, And Development, Brianne Ashley Addison

Dissertations

Mothers can alter offspring phenotypes through a variety of indirect effects, including the deposition of nutrients, hormones, and defense proteins in to the egg. Defense proteins, and antibodies in particular, may be tremendously important for neonatal defense against pathogens and the direction of resources into growth rather than immune responsiveness. Moreover, maternally derived immunoglobulins have been proposed to have an imprinting effect on the development of humoral immunity. In my dissertation, I explored a variety of ecological, life history, and developmental factors that could contribute to the evolution of yolk antibody allocation in a variety of avian species. In the …

Foraging Preference By Wild Deer On Toyon Populations From Santa Catalina Island Versus The Santa Monica Mountains, Amelia I. Clark, Rodney Honeycutt, Anjel Helms, Stephen Davis

Biology

Post-fire regeneration of Toyon (Heteromeles arbutifolia), a chaparral shrub on Santa Catalina Island is impeded by mule deer (Odocoileus hemionus), a non-native species introduced during the 1930’s. Mainland and island populations of Toyon were examined for potential differences in defense mechanisms that act as natural deterrents to over-browsing by ungulate herbivores, with the hypothesis that island populations, void of natural ungulate herbivores, may lack such defense mechanisms. Several characteristics of leaf morphology, including number and length of spines, leaf area and depth, and tensile strength of leaves, were compared between island and mainland populations. Leaves of Toyon from Santa Catalina …

Complex Evolution Of A Highly-Conserved Microsatellite Locus In Several Fish Species, J.-X. Liu, Bert Ely

Faculty Publications

The evolutionary dynamics of a highly conserved microsatellite locus (Dla 11) were studied in several fish species. The data indicated that multiple types of compound microsatellites arose through point mutations that were sometimes followed by expansion of the derived motif. Furthermore, extensive length variation was detected among species in the regions immediately flanking the repeat region.

Tropical Dry Forest Succession And The Contribution Of Lianas To Wood Area Index (Wai), Arturo Sanchez-Azofeifa, Margaret Kalacska, Mario Marcos Do Espirito-Santo, G. Wilson Fernandes, Stefan A. Schnitzer

Biological Sciences Faculty Research and Publications

The transmission and interception of light through the canopy is an important indicator of forest productivity in tropical forest ecosystems, and the amount of light that eventually reaches the forest floor is influenced by its interactions with leaves, branches, fruits, and flowers among many different canopy elements. While most studies of forest canopy light interception focus on leaf area index (LAI), very few studies have examined wood area index (WAI), which may account for a substantial component of light interception in tropical forests. The influence of lianas on the interception of light and their overall contribution to WAI is a …

Large-Scale Patterns In Trematode Richness And Infection Levels In Marine Crustacean Hosts, David W. Thieltges, Brian L. Fredensborg, Anja Studer, Robert Poulin

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Little is known about the patterns of variation in parasitism in marine hosts. Trematodes, the dominant parasites in intertidal systems, are transmitted from their first intermediate hosts (snails) to a range of second intermediate hosts, including crustaceans. Using published studies of trematode infections in crustacean hosts, we investigated general patterns of variation in trematode species richness and infection levels (i.e. percentage of hosts infected and mean number of individual parasites per host). Since the production and release of infective stages in snails is strongly temperature dependent, we also investigated a potential decrease in trematode infection levels with increasing latitude (as …

Reptilian Heart Development And The Molecular Basis Of Cardiac Chamber Evolution, K. Koshiba-Takeuchi, A. D. Mori, B. L. Kaynak, J. A. Cebra-Thomas, T. Sukonnik, R. O. Georges, S. Latham, L. Beck, R. M. Henkelman, B. L. Black, E. N. Olson, J. Wade, J. K. Takeuchi, M. Nemer, Scott F. Gilbert, B. G. Bruneau

Biology Faculty Works

The emergence of terrestrial life witnessed the need for more sophisticated circulatory systems. This has evolved in birds, mammals and crocodilians into complete septation of the heart into left and right sides, allowing separate pulmonary and systemic circulatory systems, a key requirement for the evolution of endothermy(1-3). However, the evolution of the amniote heart is poorly understood. Reptilian hearts have been the subject of debate in the context of the evolution of cardiac septation: do they possess a single ventricular chamber or two incompletely septated ventricles(4-7)? Here we examine heart development in the red-eared slider turtle, Trachemys scripta elegans (a …

Geographical Patterning Of Sixteen Goat Breeds From Italy, Albania And Greece Assessed By Single Nucleotide Polymorphisms, Lorraine Pariset, Antonella Cuteri, Christina Ligda, Alessio Valentini, Econogene Consortium

Biological Sciences Faculty Publications

Background: SNP data of goats of three Mediterranean countries were used for population studies and reconstruction of geographical patterning. 496 individuals belonging to Italian, Albanian and Greek breeds were genotyped to assess the basic population parameters.

Results: A total of 26 SNPs were used, for a total of 12,896 genotypes assayed. Statistical analysis revealed that breeds are not so similar in terms of genetic variability, as reported in studies performed using different markers. The Mantel test showed a strongly significant correlation between genetic and geographic distance. Also, PCA analysis revealed that breeds are grouped according to geographical origin, …

Spatial And Seasonal Variations In Mercury Methylation And Microbial Community Structure In A Historic Mercury Mining Area, Yolo County, California, J. M. Holloway, M. B. Goldhaber, K. M. Scow, Rebecca E. Drenovsky

Biology

The relationships between soil parent lithology, nutrient concentrations, microbial biomass and community structure were evaluated in soils from a small watershed impacted by historic Hg mining. Upland and wetland soils, stream sediments and tailings were collected and analyzed for nutrients (DOC, SO4=, NO3−), Hg, MeHg, and phospholipid fatty acids (PLFA). Stream sediment was derived from serpentinite, siltstone, volcanic rocks and mineralized serpentine with cinnabar, metacinnabar and other Hg phases. Soils from different parent materials had distinct PLFA biomass and community structures that are related to nutrient concentrations and toxicity effects of trace metals including Hg. The formation of MeHg appears …

A Hump-Shaped Relationship Between Isolation And Abundance Of Notonecta Irrorata Colonists In Aquatic Mesocosms, Shannon J. Mccauley, Christopher J. Davis, Jennifer Nystrom, Earl E. Werner

Biological Sciences

We examined the relationship between the isolation of experimental aquatic mesocosms and the abundance of an aquatic insect colonist, Notonecta irrorata, over two years. We used a curve-fitting approach to assess whether linear or quadratic models better describe the relationship between isolation and abundance. For two measures of mesocosm isolation, distance to nearest source and distance to the largest source population, there was a significant quadratic relationship between isolation and abundance. Abundance of colonizing N. irrorata was not found to be significantly related to a third measure of isolation, mesocosm connectivity. These results indicate that the relationship between habitat …

Density Dependence And The Economic Efficacy Of Marine Reserves, Crow White

Biological Sciences

Predictions on the efficacy of marine reserves for benefiting fisheries differ in large part due to considerations of models of either intra- or inter-cohort population density regulating fish recruitment. Here, I consider both processes acting on recruitment and show using a bioeconomic model how for many fisheries density dependent recruitment dynamics interact with harvest costs to influence fishery profit with reserves. Reserves consolidate fishing effort, favoring fisheries that can profitably harvest low-density stocks of species where adult density mediates recruitment. Conversely, proportion coastline in reserves that maximizes profit, and relative improvement in profit from reserves over conventional management, decline with …

Five Year Management Strategy For The Recreational Trout Fishery. Discussion Paper For Public Comment On Future Management., The Rfac’S Recreational Freshwater Fisheries Stakeholder Sub-Committee

Fisheries Management Papers

The Recreational Fishing Advisory Committee (RFAC) was concerned over the delay in time in progressing this issue and recommended that the issue of trout stocking for recreational purposes be progressed in isolation. This matter was referred to the Recreational Freshwater Fisheries Stakeholder Sub-Committee (RFFSS) to progress the recreational aspects of stocking trout within State public waters. This strategy represents a significant step in ensuring the appropriate level of management is provided for the translocation of trout into and within Western Australia for recreational purposes. It is also hoped that this policy will serve as a guide for the management and …

Desulfovibrio Idahonensis Sp. Nov., Sulfatereducing Bacteria Isolated From A Metal(Loid)-Contaminated Freshwater Sediment, H. Sass, S. Ramamoorthy, C. Yarwood, H. Langner, P. Schumann, R. M. Kroppenstedt, S. Spring, R. Frank Rosenzweig

Biological Sciences Faculty Publications

Two novel sulfate-reducing bacteria, strains CY1 and CY2, were isolated from heavy-metal-contaminated sediments of Lake Coeur d'Alene, Idaho, USA. Strains CY1 and CY2 were found to contain c-type cytochromes and to reduce sulfate, sulfite, thiosulfate, elemental sulfur, DMSO, anthraquinone disulfonate and fumarate using lactate as an electron donor. In a comparison of 16S rRNA gene sequences, CY1 and CY2 were found to be 100% identical, but only 97 and 92.4% similar, respectively, to the type strains of Desulfovibrio mexicanus and Desulfovibrio aminophilus. Unlike these species, however, CY1 was neither able to disproportionate thiosulfate nor able to use yeast extract or …

Differential Inhibition Of Various Adenylyl Cyclase Isoforms And Soluble Guanylyl Cyclase By 2',3'-O-(2,4,6-Trinitrophenyl)-Substituted Nucleoside 5'-Triphosphates, Srividya Suryanarayana, Martin Göttle, Melanie Hübner, Andreas Gille, Tung-Chung Mou, Stephen R. Sprang, Mark Richter, Roland Seifert

Biological Sciences Faculty Publications

Adenylyl cyclases (ACs) catalyze the conversion of ATP into the second messenger cAMP and play a key role in signal transduction. In a recent study (Mol Pharmacol 70: 878-886, 2006), we reported that 2',3'-O-(2,4,6-trinitrophenyl)-substituted nucleoside 5'-triphosphates (TNP-NTPs) are potent inhibitors (K(i) values in the 10 nM range) of the purified catalytic subunits VC1 and IIC2 of membranous AC (mAC). The crystal structure of VC1: IIC2 in complex with TNP-ATP revealed that the nucleotide binds to the catalytic site with the TNP-group projecting into a hydrophobic pocket. The aims of this study were to analyze the interaction of TNP-nucleotides with VC1: …

Mimicking Fire For Post-Mining Restoration Success, Katherine M. Wilkin

Master's Theses

This study is based at Rocky Canyon Quarry (RCQ), a 200-acre granite aggregate open-pit quarry with chaparral-dominated plant communities located in San Luis Obispo County, CA. At RCQ, the Surface Mining and Reclamation Act (SMARA) of 1975 was interpreted as restoring the landscape to native plant communities. Native plant community restoration projects have occurred there since 1993 through cooperation with California Polytechnic State University Biology Department in San Luis Obispo, CA. I evaluated past restoration at RCQ and researched new techniques to improve chaparral restoration based on the natural processes of fire.

Chaparral is an important fire-dominated plant community within …

Sex And Microhabitat Influence The Allocation Of Mycosporine-Like Amino Acids To Tissues In The Purple Sea Urchin, Strongylocentrotus Purpuratus, Sarah A. Gravem

Master's Theses

Field surveys of Strongylocentrotus purpuratus demonstrated that concentrations of natural sunscreens, mycosporine-like amino acids (MAAs), were higher in females than males for both gonadal and epidermal tissues, increased in ovaries as spawning season approached, and were influenced by the sea urchins’ microhabitat. Sea urchins occupying burrows, or “pits”, had lower concentrations of MAAs than those outside pits, suggesting a trade-off between physical and UV protection. Overall, UV irradiance did not influence MAA accumulation in gonadal tissues. However, males increased their allocation of MAAs to epidermal tissues in the microhabitat with the highest irradiance. Relative concentrations of individual MAAs were similar …

Impact Of Weed Management Practices On Grapevine Growth, Yield Components, Plant And Arthropod Abundance, And Carabid Seed Predation In Paso Robles Vineyard, Paolo Sanguankeo

Master's Theses

In the Central Coast of California, USA, wine grape growers are making efforts to identify weed control practices that promote biodiversity in their vineyards while maintaining yields. A field study was conducted in Paso Robles, CA in 2006 and 2007 evaluating the effect on Zinfandel grape-vine growth and production, groundcover plant, and ground dwelling arthropod communities of five weed control practices: 1) flumioxazin, 2) simazine, 3) cultivation, 4) cover crop, and 5) untreated control.

The herbicide treatments had the lowest weed biomass followed by the cultivation, being approximately 10 and 2 times lower than the weed biomass of either the …

Time's Arrow Flies Like A Bird: Two Paradoxes For Avian Circadian Biology, Vincent M. Cassone, Jiffin K. Paulose, Melissa G. Whitfield-Rucker, Jennifer L. Peters

Biology Faculty Publications

Biological timekeeping in birds is a fundamental feature of avian physiology, behavior and ecology. The physiological basis for avian circadian rhythmicity has pointed to a multi-oscillator system of mutually coupled pacemakers in the pineal gland, eyes and hypothalamic suprachiasmatic nuclei (SCN). In passerines, the role of the pineal gland and its hormone melatonin is particularly important. More recent molecular biological studies have pointed to a highly conserved mechanism involving rhythmic transcription and translation of "clock genes". However, studies attempting to reconcile the physiological role of pineal melatonin with molecular studies have largely failed. Recent work in our laboratory has suggested …

Fisheries Occasional Publication No.70 - Stakeholder Survey 2008, Tara Baharthah

Fisheries Occasional Publications

A telephone survey of Department of Fisheries’ stakeholders was conducted in August 2008 in order to evaluate their perception of the Department’s management of commercial and recreational fishing, pearling and aquaculture, and fish and fish habitat protection. The survey also assessed satisfaction with the level of service provided to stakeholders and their involvement in decision making processes. The results of this survey were compared to similar surveys conducted during 2002, 2004, 2006 and a survey of the general public conducted during 2008. The sample comprised of 149 interviews – 39 representing the commercial fishing sector, 41 representing the recreational fishing …

Plasma Testosterone Correlates With Morph Type Across Breeding Substages In Male White-Throated Sparrows, M. B. Swett, Creagh W. Breuner

Biological Sciences Faculty Publications

White-throated sparrows (Zonotrichia albicollis) exhibit a genetic polymorphism that affects plumage and behavior in both sexes. White-striped morphs are more territorially aggressive, whereas tan-striped morphs provision nestlings at a higher rate. We investigated testosterone physiology in this species in an effort to understand hormonal mechanisms for the observed differences in aggression and parental care between the morphs. We found a small but significant difference in plasma testosterone between free-living white-striped and tan-striped males over the course of the breeding season. This difference correlates with previously observed differences in aggressive behavior and suggests that testosterone may mediate these differences. Testosterone remained …

Sibship Reconstruction Demonstrates The Extremely Low Effective Population Size Of Striped Bass Morone Saxatilis In The Santee-Cooper System, South Carolina, Usa, Jin-Xian Liu, Bert Ely

Faculty Publications

For organisms with great fecundity and high mortality in early life stages, such as shellfish or fishes, the need to match reproductive activity with environmental conditions conducive to spawning, fertilization, larval development and recruitment may result in extreme variance in reproductive success among individuals. The main objective of this study was to investigate evidence of large variance in the reproductive success of the striped bass Morone saxatilis in the Santee-Cooper system, South Carolina, USA. Seven microsatellite loci were analysed in 603 recruits representing three yearly cohorts from 1992 to 1994, and a group analysis was performed to identify full-sib families. …