Biology Commons^™

Antimicrobial Activity Of The Lichen Cetraria Islandica (L.) Ach., Başaran Dülger, Fahrettin Güci̇n, Ali Aslan

Turkish Journal of Biology

In this study, extracts of Cetraria islandica (L.) Ach. were prepared with Ethyl acetate, Acetone, Chloroform and Ethanol and antimicrobial activities of these extracts were examined on test microorganisms as follows: Escherichia coli ATCC 11230, Enterobacter aerogenes CCM 2531, Staphylococcus aureus 6538P, Staphylococcus epidermidis, Bacillus subtilis La2114, Bacillus cereus var. mycoides, Bacillus sphaericus, Bacillus thurigiensis, Bacillus megaterium, Mycobacterium smegmatis RUT, Salmonella thyphimurium, Candida utilis La991, Candida albicans CCM314, Aspergillus flavus, Aspergillus oryzae, Aspergillus fumigatus, Trichophyton rubrum, Botrytis cineriae, Fusarium oxysporium, Streptomyces murinus ISP 5091 and Nocardia cornea IFO 14403 by Disc Diffusion Methods. As a result of study, we have …

The Investigation Of Antimicrobial Effect Of Some Plant Extract, Ahmet Ilçim, Metin Diğrak, Eyüp Bağci

Turkish Journal of Biology

The Parmelia furfuracea (L) Zopf. (Liken), Crocus chrysanthus (Herbert) Herbert (Çigdem), Rumex scutatus L., (Kuzukulagi), Myrtus communis L. subsps. communis Ic: Sibth. and Sm. (Mersin), Asphodelus aestivus L. (Çiris) Eugenia caryophyllata Thunb. (Karanfil) which were collected from Kahramanmaras and Hatay region have been investigated in respect of their antimicrobial and antifungal activities against different test organisms ( Bacillus megaterium DSM 32, Bacillus subtilis IMG 22, Bacillus, brevis FMC 3, Escherichia coli DM, Klebsiella pneumoniae FMC 5, Enterobacter aerogenes CCM 2531, Pseudomonas aeruginosa DSM 50071, Staphylococcus aureus COWAN 1, Listeria monocytogenes SCOTT A, bacteria and Candida albicans CCM 314 and Saccharomyces …

The Construction Of A Mammalian Transfectionvector For Expression Of Cytosine-5 Specific Dnamethyltransferase Gene M.Msp1 In Cultured Cells, Öztürk Özdemi̇r

Turkish Journal of Biology

The expression vectors are designed for expression and purification of normal or recombi-nant gene of interest. There is a wide variety of stable and transferable selectable mammalian expres-sion vectors.The vector pCDM8 and its derivatives, pcDNAI/Ampicilline are widely used for cloning and analyzing of genes in higher eukaryotic cells. The vectors pRC/CMV, pcDNA3 and pRC/RSV are designed for high-level expression of recombinant genes in mammalian cells. In the present study, we have been able to transfect and express the monospecific bacterial (Moroxcella spp) cytosine-5 DNA methyltransferase (MTase) M.Msp1 gene in cultured cells within a newly con-structed mammalian transfection vector. We have …

Biochemical Characterization Of Elastase Frompseudomonas Aeruginosa Ses 938-1, Semra Kocabiyik, Ezgi Ergi̇n

Turkish Journal of Biology

We have investigated some biochemical properties of elastase from a new strain o f Pseudomonas aeruginosa (SES 938-1). Activities were measured at 410 nm using N-succinyl-L-(ala) 3 -p- nitroanilide as substrate. The elastase activity followed Michaelis-Menten kinetics over the substrate range of 0.067-0.540 mM with the apparent K m value of 0.375 mM. Optimum activity was observed at 36 °C and pH 7.5. elastase activity was inhibited by metal chelating agents and high concentrations of Mn 2+ , Zn 2+ and Ni 2+ . The results obtained suggest that elastase from P. aeruginosa SES 938-1 is a neutral metalloproteinase.

Serological Properties Of Pseudomonas Syringaepvphaseolicolaisolates Collected From Eskisehir, Dilek Ertugrul, Kıymet Güven

Turkish Journal of Biology

Thirty six bean seed samples collected from bean fields in Eskisehir-Türkiye were examined for detection and isolation of Pseudomonas syringae pv. phaseolicola. A polyclonal antiserum specific to P. s. pv. phaseolicola NCPPB 52 was raised in a rabbit and both biochemical and serological test were used for identification. Heat-stable O antigens of 92 isolates identified as P. S. pv. phaseolicola together with authentic isolates of the bacterium were compared by Ouchterlony immunodiffusion test and all the isolates had a common antigenic structure.

The Effects Of Phenoxybenzamine And Cold Stresson Tyrosine Hydroxylase Enzyme Activity Andexpression Of Tyrosine Hydroxylase Gene In The Ratadrenal Medulla, Muhittin Yürekli

Turkish Journal of Biology

The effects of cold exposure and cold plus phenoxybenzamine treatment on tyrosine hydrox-ylase (TH) activity and TH m RNA levels were investigated in the adrenal medulla of Sprague Dawley (SD) rats. SD rats were exposed to cold (4 °C) for 48 hours. Phenoxybenzamine was injected intra-peritoneally (i.p) at 20 mg/kg, which was prepared in 5% ethanol containing 0.9 % NaCL TH activity was measured by monitoring the formation of 3 H 2 O as a product of L-(3 H)-tyrosine hydroxylation, the formation of L-(3 H)-dopa from L-(3 H)-tyrosine. Total adrenal RNA was isolated and blotted onto a nylon membrane and …

A Theoretical Study On The Sex-Pheromones Of Therice Moth, Corcyra Cephalonica Stainton, Lemi Türker

Turkish Journal of Biology

Molecular orbital calculations (AM1 type) were performed on the geometry optimized structures of various farnesal isomers and methyl esters of farnesoic acids. It has been found that the major component, E, E-farnesal, of the sex-pheromone for the rice moth, Corcyra cephalonica Stainton is chemically the most exothermic and stable of all the farnesal isomers. Then, the resem-blance of molecular shapes was detrmined in order to design more stable sex-attractants.

Larval Growth And Survival Of Penaeus Indicus(Decapoda: Penaeidae) On Live Feeds, Metin Kumlu

Turkish Journal of Biology

This study was conducted to compare the suitability of three microalgal species (Tetraselmis chuii, Skeletonema costatum and Rhinomonas reticulata), both individually and in combination, as food for Penaeus indicus during larval culture. To determine the best feeding density, each alga was fed to protozoea 1 (PZI) larvae from 10 to 80 cells mL -1 . The best algal-cell density, promoting the highest survival rate, greatest growth and fastest larval development, was obtained at 60-70 cells mL -1 with a combination of T. chuii and S. costatum. Low cell densities (10-20 cells mL -1 ) were not effective. The diatom S. …

Heat Shock Protein Synthesis In The Gorgonian Leptogorgia Virgulata, Evelyn Afif

Honors Theses

No abstract provided.

Genetic Variation Within And Among Populations Of Florida Burrowing Owls (Athene Cunicularia Floridana), Wendy T. Denton

Legacy ETDs

This study employed DNA Polymerase Chain Reaction (PGR) and Single-Stranded Conformation Polymorphisms (SSCP) on a mitochondrial control region target to assess population structure and possible gene flow in the Florida Burrowing Owl (Athene cunicularia floridana), a Florida Species of Special Concern. Although widespread in Florida, Burrowing Owls occur at low densities in semi-isolated populations that are susceptible to extirpation from human development and the demographic consequences of small population size. To better manage these populations, there is a need for data on the population genetic structure of the Florida Burrowing Owl. Therefore, I acquired DNA from Burrowing Owls …

Evaluation Of Coverboards For Sampling Salamanders In South Georgia, Charles Michael Houze Jr.

Legacy ETDs

Salamanders are important components of many forested communities. However, their fossorial habits and seasonality often make them difficult to sample. The use of artificial coverboards (coverboards) is a relatively new and little-studied technique for monitoring the terrestrial activity of salamanders Coverboards are designed to simulate fallen tree limbs and logs, and to provide a moist refuge for forest salamanders. Despite several studies, questions remain concerning the value of coverboards. Therefore, I conducted an experiment in 1996-1997 to determine whether coverboards are as effective for sampling salamanders as searching natural cover objects such as logs and branches. Using a paired design, …

Spiroplasma Diversity In Two Tabanid Flies And In Vivo Interactions Of Spiroplasma And Entomoplasma, Latonya T. Brown-Derrick

Legacy ETDs

Chrysops vittatus, a small deer fly, and Tabanus atratus, a horse fly, were captured concurrently in Bulloch County, Georgia during June through September of 1989-1997 Cultures were made from adult females, and the frequency and diversity of spiroplasma carriage were determined by deformation and endpoint testing.

Of the 90 Chrysops vittatus processed, 23 (25 .6%) carried spiroplasmas. The most prevalent spiroplasmas were representatives of group Vlll (20/23): strain B1357 (7/23); Spiroplasma syrphidicola (5/23); S. chrysopicola (4/23); strain TAAS-1 (4/23). Groups XVIII and XXXV were represented by S. Htorale (2/23) and strain B2649 (1/23), respectively.

Of the …

Host-Plant Associations And Specificity Among Cynipid Gall-Inducing Wasps Of Eastern Usa, Warren Abrahamson, G. Melika, R, Scrafford, G. Csoka

Warren G. Abrahamson, II

No abstract provided.

Root Growth, Developmental Changes In The Apex, And Hydraulic Conductivity For Opuntia Ficus-Indica During Drought, J. Dubrovsky, Gretchen North, P. Nobel

Gretchen North

Developmental changes in the root apex and accompanying changes in lateral root growth and root hydraulic conductivity were examined for Opuntia ficus-indica (L.) Miller during rapid drying, as occurs for roots near the soil surface, and more gradual drying, as occurs in deeper soil layers. During 7 d of rapid drying (in containers with a 3-cm depth of vermiculite), the rate of root growth decreased sharply and most root apices died; such a determinate pattern of root growth was not due to meristem exhaustion but rather to meristem mortality after 3 d of drying. The length of the meristem, the …

Water Uptake And Structural Plasticity Along Roots Of A Desert Succulent During Prolonged Drought, Gretchen North, P. Nobel

Gretchen North

No abstract provided.

Gall-Inducing Insects Provide Insights Into Plant Systematic Relationships, Warren Abrahamson, G. Melika, R. Scrafford, G. Csoka

Warren G. Abrahamson, II

No abstract provided.

Just Lookin' For A Home, Warren G. Abrahamson Ii, A. E. Weis

Warren G. Abrahamson, II

No abstract provided.

Precocene Ii Modifies Maternal Responsiveness In The Burrower Bug, Sehirus Cinctus (Heteroptera)., Scott Kight

Scott Kight

Revised Group Classification Of The Genus Spiroplasma, David L. Williamson, Robert F. Whitcomb, Joseph G. Tully, Gail E. Gasparich, David L. Rose, Patricia Carle, Joseph M. Bové, Kevin J. Hackett, Jean R. Adams, Roberta B. Henegar, Meghnad Konai, Claude Chastel, Frank E. French

Gail Gasparich

Serologic And Genomic Relatedness Of Group Xiv Spiroplasma Isolates From A Lampyrid Beetle And Tabanid Flies: An Ecologic Paradox, G. E. Gasparich, K. J. Hackett, F. E. French, R. F. Whitcomb

Gail Gasparich