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Articles 511 - 540 of 602

Full-Text Articles in Biology

Effects Of Regional Origin And Genotype On Intraspecific Root Communication In The Desert Shrub Ambrosia Dumosa (Asteraceae), Bruce E. Mahall, Ragan M. Callaway Jan 1996

Effects Of Regional Origin And Genotype On Intraspecific Root Communication In The Desert Shrub Ambrosia Dumosa (Asteraceae), Bruce E. Mahall, Ragan M. Callaway

Biological Sciences Faculty Publications

Previous work has shown that the contact inhibition that occurs among roots of Ambrosia dumosa shrubs has a self/nonself recognition capability. In the current study, we investigated some of the geographic and genotypic dimensions of this recognition capability by using root observation chambers to observe the effects of encounters of individual roots on root elongation rates. We measured such effects in encounters between roots of plants from the same region and compared these to effects in encounters between roots of plants from two different regions. We also measured effects of encounters between roots of plants from the same clones and …

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Variation In Leaf Structure And Function In Quercus Douglasii Trees Differing In Root Architecture And Drought History, Ragan M. Callaway, Bruce E. Mahall Jan 1996

Variation In Leaf Structure And Function In Quercus Douglasii Trees Differing In Root Architecture And Drought History, Ragan M. Callaway, Bruce E. Mahall

Biological Sciences Faculty Publications

Seasonal changes in leaf specific mass, nitrogen, chlorophyll, and photosynthetic properties were measured for two groups of spatially intermixed Quercus douglasii trees with different drought histories and apparently different root architectures. One group, referred to as ''high-psi(pd) trees, included trees with low amounts of fine root biomass in the upper 50 cm of soil and high predawn xylem pressure potentials (psi(pd)) during summer drought. These two characteristics indicate that trees in this group have deep roots, which may reach the water table. The second group, referred to as ''low-psi(pd) trees, had three to five times higher fine root biomass in …

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The Structure Of The G Protein Heterotrimer GIα1Β1Γ2, Mark A. Wall, David E. Coleman, Ethan Lee, Jorge A. Iñiguez-Lluhi, Bruce A. Posner, Alfred G. Gilman, Stephen R. Sprang Dec 1995

The Structure Of The G Protein Heterotrimer GIα1Β1Γ2, Mark A. Wall, David E. Coleman, Ethan Lee, Jorge A. Iñiguez-Lluhi, Bruce A. Posner, Alfred G. Gilman, Stephen R. Sprang

Biological Sciences Faculty Publications

The crystallographic structure of the G protein heterotrimer Giα1(GDP)β1γ2 (at 2.3 A) reveals two nonoverlapping regions of contact between α and β, an extended interface between β and nearly all of γ, and limited interaction of α with γ. The major α/β interface covers switch II of α, and GTP-induced rearrangement of switch II causes subunit dissociation during signaling. Alterations in GDP binding in the heterotrimer (compared with α-GDP) explain stabilization of the inactive conformation of α by βγ. Repeated WD motifs in β form a circularized sevenfold β propeller. The conserved cores of these …

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2,4-Dichlorophenoxyacetic Acid-Degrading Bacteria Contain Mosaics Of Catabolic Genes, R. R. Fulthorpe, C. Mcgowan, O. V. Maltseva, William E. Holben, J. M. Tiedje Sep 1995

2,4-Dichlorophenoxyacetic Acid-Degrading Bacteria Contain Mosaics Of Catabolic Genes, R. R. Fulthorpe, C. Mcgowan, O. V. Maltseva, William E. Holben, J. M. Tiedje

Biological Sciences Faculty Publications

DNA from 32 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacteria from diverse locations was probed with the first three genes of the well-known 2,4-D degradation pathway found in Alcaligenes eutrophus JMP134(pJP4). The majority of strains did not show high levels of homology to the first three genes of the 2,4-D degradation pathway, tfdA, -B, and -C. Most strains showed combinations of tfdA-, B-, and C-like elements that exhibited various degrees of homology to the gene probes. Strains having the same genomic fingerprints (as determined by repetitive extragenic palindromic PCR) exhibited the same hybridization pattern regardless of the geographic origin of the …

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How Ras Works: Structure Of A Rap-Raf Complex, Stephen R. Sprang Jul 1995

How Ras Works: Structure Of A Rap-Raf Complex, Stephen R. Sprang

Biological Sciences Faculty Publications

The three-dimensional structure of the complex between Rap and the 'Ras-binding domain' of Raf could be the prototype for a G protein-effector interaction.

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Crystallographic Evidence For Dimerization Of Unliganded Tumor Necrosis Factor Receptor, James H. Naismith, Tracey Q. Devine, Barbara J. Brandhuber, Stephen R. Sprang Jun 1995

Crystallographic Evidence For Dimerization Of Unliganded Tumor Necrosis Factor Receptor, James H. Naismith, Tracey Q. Devine, Barbara J. Brandhuber, Stephen R. Sprang

Biological Sciences Faculty Publications

Activation of the cell surface receptors for tumor necrosis factor (TNF) is effected by the aggregation of cytoplasmic domains that occurs when the extracellular domains of two or three receptors bind to trimeric TNFα or TNFβ. The structure of the type I TNF receptor extracellular domain (sTNF- R1), crystallized in the absence of TNF, has now been determined at 2.25-Å resolution. The receptor itself is an elongated molecule comprising four disulfide-rich domains in a nearly linear array. Contrary to expectations, the unliganded domains are found to associate into dimers of two distinct types, in which monomers are related by local …

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Effects Of Reproductive Mode On Demography And Life History In Arabis Fecunda (Brassicaceae), Peter Lesica, J. Stephen Shelly Jun 1995

Effects Of Reproductive Mode On Demography And Life History In Arabis Fecunda (Brassicaceae), Peter Lesica, J. Stephen Shelly

Biological Sciences Faculty Publications

Life history theory predicts that trade-offs between growth and reproduction should be dictated by a population's mortality schedule. We tested this prediction with Arabis fecunda, a short-lived perennial that occurs in many different habitats in southwest Montana. Individuals produce either or both axillary or terminal inflorescences. Axillary-flowering plants are usually iteroparous and have smaller reproductive bouts, while terminal-flowering plants have larger reproductive bouts, and tend to be semelparous. We recorded size and fecundity of A. fecunda individuals from 1989 to 1993 in three different habitats. There was great variation in demographic and life history traits among the populations. A …

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Characterization Of Diverse 2,4-Dichlorophenoxyacetic Acid-Degradative Plasmids Isolated From Soil By Complementation, Eva M. Top, William E. Holben, Larry J. Forney May 1995

Characterization Of Diverse 2,4-Dichlorophenoxyacetic Acid-Degradative Plasmids Isolated From Soil By Complementation, Eva M. Top, William E. Holben, Larry J. Forney

Biological Sciences Faculty Publications

The diversity of 2,4-dichlorophenoxyacetic acid (2,4-D)-degradative plasmids in the microbial community of an agricultural soil was examined by complementation. This technique involved mixing a suitable Alcaligenes eutrophus (Rifr) recipient strain with the indigenous microbial populations extracted from soil. After incubation of this mixture, Rifr recipient strains which grow with 2,4-D as the only C source were selected. Two A. eutrophus strains were used as recipients: JMP228 (2,4-D-), which was previously derived from A. eutrophus JMP134 by curing of the 2,4-D-degradative plasmid pJP4, and JMP228 carrying pBH501aE (a plasmid derived from pJP4 by deletion of a …

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Maintaining Bird Diversity In Western Larch/Douglas-Fir Forests, Bret W. Tobalske, Raymond C. Shearer, Richard L. Hutto May 1995

Maintaining Bird Diversity In Western Larch/Douglas-Fir Forests, Bret W. Tobalske, Raymond C. Shearer, Richard L. Hutto

Biological Sciences Faculty Publications

Bird occurrences were evaluated under four stand conditions in western larch/Douglas-fir forests: clearcut, partial cut, unlogged (fragmented), and contiguous forest. Frequencies were noted for foraging guilds, tree gleaners, flycatchers, nesting guilds, tree drillers, and primary cavity nesters. Managers should consider a diversity of habitat conditions if maintaining habitat for bird species is an objective.

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Structure Of The First C2 Domain Of Synaptotagmin I: A Novel Ca(2+)/Phospholipid-Binding Fold, R. Bryan Sutton, Bazbek A. Davletov, Albert M. Berghuis, Thomas C. Sudhof, Stephen R. Sprang Mar 1995

Structure Of The First C2 Domain Of Synaptotagmin I: A Novel Ca(2+)/Phospholipid-Binding Fold, R. Bryan Sutton, Bazbek A. Davletov, Albert M. Berghuis, Thomas C. Sudhof, Stephen R. Sprang

Biological Sciences Faculty Publications

C2 domains are regulatory sequence motifs that occur widely in nature. Synaptotagmin I, a synaptic vesicle protein involved in the Ca2+ regulation of exocytosis, contains two C2 domains, the first of which acts as a Ca2+ sensor. We now describe the three-dimensional structure of this C2 domain at 1.9 Å resolution in both the Ca2+-bound and Ca2+-free forms. The C2 polypeptide forms an eight-stranded β sandwich constructed around a conserved four-stranded motif designated as a C2 key. Ca2+ binds in a cup-shaped depression between two polypeptide loops located …

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Effects Of Silvicultural Treatments In The Rocky Mountains, Sallie J. Hejl, Richard L. Hutto, Charles R. Preston, Deborah M. Finch Jan 1995

Effects Of Silvicultural Treatments In The Rocky Mountains, Sallie J. Hejl, Richard L. Hutto, Charles R. Preston, Deborah M. Finch

Biological Sciences Faculty Publications

We offer a description of current forest structure and basic information on bird distribution across forest habitats in the Rockies as background knowledge for understanding the effects of silviculture on birds in these habitats. We also offer a comparison between the effects of silviculture, and the effects of fire and fire suppression on forest birds in an attempt to give a holistic perspective on the health of forest birds' in the Rockies.

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A Comparison Of Bird Detection Rates Derived From On-Road Versus Off-Road Point Counts In Northern Montana, Richard L. Hutto, Sallie J. Hejl, Jeffrey F. Kelly, Sandra M. Pletschet Jan 1995

A Comparison Of Bird Detection Rates Derived From On-Road Versus Off-Road Point Counts In Northern Montana, Richard L. Hutto, Sallie J. Hejl, Jeffrey F. Kelly, Sandra M. Pletschet

Biological Sciences Faculty Publications

We conducted a series of 275 paired (on- and off-road) point counts within 4 distinct vegetation cover types in northwestern Montana. Roadside counts generated a bird list that was essentially the same as the list generated from off-road counts within the same vegetation cover type. Species that were restricted to either on- or off-road counts were rare, suggesting that restricted occurrences were a consequence of small sample sizes and not a product of habitat differences surrounding on-versus off-road points. Nevertheless, there were significant differences in the mean number of individuals detected between on- and off-road points for a number of …

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Genetic Transformation Of The Lyme Disease Agent Borrelia Burgdorferi With Coumarin-Resistant Gyrb., D. Scott Samuels, Kathleen E. Mach, Claude F. Garon Oct 1994

Genetic Transformation Of The Lyme Disease Agent Borrelia Burgdorferi With Coumarin-Resistant Gyrb., D. Scott Samuels, Kathleen E. Mach, Claude F. Garon

Biological Sciences Faculty Publications

No useful method to genetically manipulate Borrelia burgdorferi, the causative agent of Lyme disease, has been developed previously. We have used resistance to the coumarin antibiotic coumermycin A1, an inhibitor of DNA gyrase, as a genetic marker to monitor the transformation of B. burgdorferi by electroporation. Introduction of site-directed mutations into the gyrB gene demonstrated that transformation was successful, provided evidence that homologous recombination occurs on the chromosome, and established that mutations at Arg-133 of DNA gyrase B confer coumermycin A1 resistance in B. burgdorferi. The coumermycin A1-resistant gyrB marker and genetic transformation can now be applied toward dissecting the …

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Mechanism Of Gtp Hydrolysis By G-Protein Α Subunits, Christiane Kleuss, André S. Raw, Ethan Lee, Stephen R. Sprang, Alfred G. Gilman Oct 1994

Mechanism Of Gtp Hydrolysis By G-Protein Α Subunits, Christiane Kleuss, André S. Raw, Ethan Lee, Stephen R. Sprang, Alfred G. Gilman

Biological Sciences Faculty Publications

Hydrolysis of GTP by a variety of guanine nucleotide-binding proteins is a crucial step for regulation of these biological switches. Mutations that impair the GTPase activity of certain heterotrimeric signal-transducing G proteins or of p21(ras) cause tumors in man. A conserved glutamic residue in the α subunit of G proteins has been hypothesized to serve as a general base, thereby activating a water molecule for nucleophilic attack on GTP. The results of mutagenesis of this residue (Glu-207) in G(iα1) refute this hypothesis. Based on the structure of the complex of G(iα1) with GDP, Mg2+, and AlF4/ …

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Analysis Of The Distribution And Molecular Heterogeneity Of The Ospd Gene Among The Lyme Disease Spirochetes: Evidence For Lateral Gene Exchange, Richard T. Marconi, D. Scott Samuels, Robert K. Landry, Claude F. Garon Aug 1994

Analysis Of The Distribution And Molecular Heterogeneity Of The Ospd Gene Among The Lyme Disease Spirochetes: Evidence For Lateral Gene Exchange, Richard T. Marconi, D. Scott Samuels, Robert K. Landry, Claude F. Garon

Biological Sciences Faculty Publications

Analysis of the ospD gene has revealed that this gene is not universal among Lyme disease spirochete isolates. The gene was found to be carried by 90, 50, and 24% of the Borrelia garinii, B. afzelii, and B. burgdorferi isolates tested. Size variability in the ospD-encoding plasmid was also observed. Sequence analysis has demonstrated the presence of various numbers of a 17-bp repeated sequence in the upstream control (promoter) region of the gene. In addition, a region within the coding sequence where various insertions, deletions, and direct repeats occur was identified. ospD gene sequences from 31 different isolates were determined …

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Identification Of Outer-Membrane Proteins Of Bartonella-Bacilliformis, Michael F. Minnick Jun 1994

Identification Of Outer-Membrane Proteins Of Bartonella-Bacilliformis, Michael F. Minnick

Biological Sciences Faculty Publications

Purification of the outer membrane of Bartonella bacilliformis by sucrose step gradient centrifugation and analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) suggest that 14 proteins, ranging from 11.2 to 75.3 kDa, are located in the outer membrane of the pathogen. On the basis of M(r)s, eleven of these proteins have counterparts which are labeled by extrinsic radioiodination of intact bartonellae, and two of the proteins are visibly sensitive to extrinsic proteinase IC digestion in analysis by SDS-PAGE. While nearly all the extrinsically radioiodinated proteins could be immunoprecipitated with rabbit antibartonella hyperimmune serum, proteins of 31.5, 42, and 45 kDa …

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Gyrb Mutations In Coumermycin A1-Resistant Borrelia Burgdorferi., D. Scott Samuels, Richard T. Marconi, Wai Mun Huang, Claude F. Garon May 1994

Gyrb Mutations In Coumermycin A1-Resistant Borrelia Burgdorferi., D. Scott Samuels, Richard T. Marconi, Wai Mun Huang, Claude F. Garon

Biological Sciences Faculty Publications

We have isolated and characterized mutants of Borrelia burgdorferi that are resistant to the antibiotic coumermycin A1, which targets the B subunit of DNA gyrase. Mutants had either 100- or 300-fold higher resistance to coumermycin A1 than wild-type B. burgdorferi. In each case, a single point mutation in the gyrB gene converted Arg-133 to Gly or Ile. Mutations in the homologous Arg residue of Escherichia coli DNA gyrase are also associated with resistance to coumarin antimicrobial agents.

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Use Of Gene Probes To Aid In Recovery And Identification Of Functionally Dominant 2,4-Dichlorophenoxyacetic Acid-Degrading Populations In Soil, J. O. Ka, William E. Holben, James M. Tiedje Apr 1994

Use Of Gene Probes To Aid In Recovery And Identification Of Functionally Dominant 2,4-Dichlorophenoxyacetic Acid-Degrading Populations In Soil, J. O. Ka, William E. Holben, James M. Tiedje

Biological Sciences Faculty Publications

The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) was applied to soils in microcosms, and degradation was monitored after each of five repeated additions. Total DNAs were isolated from soil bacterial communities after each 2,4-D treatment. The DNA samples were analyzed on slot blots and Southern blots by using a tfdA gene probe subcloned from plasmid pJP4 and a Spa probe derived from a different 2,4-D-degrading isolate, a Sphingomonas paucimobilis strain. 2,4-D applied to soil was quickly degraded by indigenous microbial populations. As determined by slot blot analyses of DNA from a Michigan soil, the increase in hybridization signal in response to 2,4-D …

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Analysis Of Competition In Soil Among 2,4-Dichlorophenoxyacetic Acid-Degrading Bacteria, J. O. Ka, William E. Holben, James M. Tiedje Apr 1994

Analysis Of Competition In Soil Among 2,4-Dichlorophenoxyacetic Acid-Degrading Bacteria, J. O. Ka, William E. Holben, James M. Tiedje

Biological Sciences Faculty Publications

Competition among indigenous and inoculated 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacteria was studied in a native Kansas prairie soil following 2,4-D additions. The soil was inoculated with four different 2,4-D-degrading strains at densities of 10(3) cells per g of soil; the organisms used were Pseudomonas cepacia DBO1(pJP4) and three Michigan soil isolates, strain 745, Sphingomonas paucimobilis 1443, and Pseudomonas pickettii 712. Following 2,4-D additions, total soil DNA was extracted and analyzed on Southern blots by using a tfdA gene probe which detected three of the strains and another probe that detected the fourth strain, S. paucimobilis 1443, which belongs to a different …

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Genetic And Phenotypic Diversity Of 2,4-Dichlorophenoxyacetic Acid (2,4-D)-Degrading Bacteria Isolated From 2,4-D-Treated Field Soils, J. O. Ka, William E. Holben, James M. Tiedje Apr 1994

Genetic And Phenotypic Diversity Of 2,4-Dichlorophenoxyacetic Acid (2,4-D)-Degrading Bacteria Isolated From 2,4-D-Treated Field Soils, J. O. Ka, William E. Holben, James M. Tiedje

Biological Sciences Faculty Publications

Forty-seven numerically dominant 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacteria were isolated at different times from 1989 through 1992 from eight agricultural plots (3.6 by 9.1 m) which were either not treated with 2,4-D or treated with 2,4-D at three different concentrations. Isolates were obtained from the most dilute positive most-probable-number tubes inoculated with soil samples from the different plots on seven sampling dates over the 3-year period. The isolates were compared by using fatty acid methyl ester (FAME) profiles, chromosomal patterns obtained by PCR amplification of repetitive extragenic palindromic (REP) sequences, and hybridization patterns obtained with probes for the tfd genes of …

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The Composition And Social-Organization Of Mixed-Species Flocks In A Tropical Deciduous Forest In Western Mexico, Richard L. Hutto Feb 1994

The Composition And Social-Organization Of Mixed-Species Flocks In A Tropical Deciduous Forest In Western Mexico, Richard L. Hutto

Biological Sciences Faculty Publications

I recorded the flocking propensity of birds within a tropical deciduous forest in western Mexico during the nonbreeding season, and determined the species composition of 57 mixed-species, canopy insectivore flocks. Each of 27 canopy insectivore species present on the study area was observed foraging in mixed-species flocks on at least half of the occasions that it was detected on bird surveys. The proportion of flocks within which a given species was detected could be predicted on the basis of its index of abundance, as determined from independently derived point count data. Therefore, flocks are not comprised of a special subset …

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Crystallization And Preliminary Crystallographic Studies Of Giα1 And Mutants Of Giα1 In The Gtp And Gdp-Bound States, David E. Coleman, Ethan Lee, Mark B. Mixon, Maurine E. Linder, Albert M. Berghuis, Alfred G. Gilman, Stephen R. Sprang Jan 1994

Crystallization And Preliminary Crystallographic Studies Of Giα1 And Mutants Of Giα1 In The Gtp And Gdp-Bound States, David E. Coleman, Ethan Lee, Mark B. Mixon, Maurine E. Linder, Albert M. Berghuis, Alfred G. Gilman, Stephen R. Sprang

Biological Sciences Faculty Publications

Several different crystal forms of Giα1 have been grown and analyzed. Crystals of native protein containing bound GTPγS belong to space group P3121 or P3221 with cell dimensions a, b = 80·6 Åand c = 106·3 Å and diffract to a resolution of 1·9 Å using synchrotron radiation. Crystals of native protein containing bound GDP belong to space group I4 with cell dimensions a, b = 121·3 Å, and c = 67·7 Å and diffract to 3·0Å. Data sets from crystals grown using mutant proteins have also been obtained and characterized.

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Two Crystal Forms Of The Extracellular Domain Of Type I Tumor Necrosis Factor Receptor, Lynn E. Rodseth, Barbara Brandhuber, Tracey Q. Devine, Michael J. Eck, Karin Hale, James H. Naismith, Stephen R. Sprang Jan 1994

Two Crystal Forms Of The Extracellular Domain Of Type I Tumor Necrosis Factor Receptor, Lynn E. Rodseth, Barbara Brandhuber, Tracey Q. Devine, Michael J. Eck, Karin Hale, James H. Naismith, Stephen R. Sprang

Biological Sciences Faculty Publications

The soluble extracellular domain of human type I tumor necrosis factor receptor (sTNFrI) is a 161 residue polypeptide found in serum and urine. This domain tightly binds tumor necrosis factors (TNF) α and β and, as part of the whole receptor, initiates the powerful biological effects of TNF. The extracellular domain, typical of other TNF receptor superfamily members, comprises four cysteine-rich motifs. We have obtained two crystal forms of the sTNFrI. One crystal form is grown at pH 3.7 with MgSO4 as the precipitant. These crystals are orthorhombic, space group P212121, with cell …

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Characterization Of Bartonella-Bacilliformis Flagella And Effect Of Antiflagellin Antibodies On Invasion Of Human Erythrocytes, David C. Scherer, Isaure Deburon-Connors, Michael F. Minnick Dec 1993

Characterization Of Bartonella-Bacilliformis Flagella And Effect Of Antiflagellin Antibodies On Invasion Of Human Erythrocytes, David C. Scherer, Isaure Deburon-Connors, Michael F. Minnick

Biological Sciences Faculty Publications

Bartonella bacilliformis is the etiologic agent of Oroya fever in humans. Flagellum-mediated motility has been postulated as a major virulence factor for invasion of host cells. To address this hypothesis, we purified and characterized flagella from strain KC584 and then assessed their role in human erythrocyte association and invasion. Electron microscopy of the flagellar preparation showed a high concentration of filaments with a mean wavelength of 800 nm. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblot analysis, and KBr density gradient centrifugation indicated that the flagellar filament is composed of a polypeptide of 42 kDa. The flagellin is partially (ca. 50%) resistant …

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Affinity Panning Of A Library Of Peptides Displayed On Bacteriophages Reveals The Binding Specificity Of Bip, Sylvie Blond-Elguindi, Steven E. Cwirla, William J. Dower, Robert J. Lipshutz, Stephen R. Sprang, Joseph F. Sambrook, Mary Jane H. Gething Nov 1993

Affinity Panning Of A Library Of Peptides Displayed On Bacteriophages Reveals The Binding Specificity Of Bip, Sylvie Blond-Elguindi, Steven E. Cwirla, William J. Dower, Robert J. Lipshutz, Stephen R. Sprang, Joseph F. Sambrook, Mary Jane H. Gething

Biological Sciences Faculty Publications

We have used affinity panning of libraries of bacteriophages that display random octapeptide or dodecapeptide sequences at the N-terminus of the adsorption protein (plll) to characterize peptides that bind to the endoplasmic reticulum chaperone BiP and to develop a scoring system that predicts potential BiP-binding sequences in naturally occurring polypeptides. BiP preferentially binds peptides containing a subset of aromatic and hydrophobic amino acids in alternating positions, suggesting that peptides bind in an extended conformation, with the side chains of alternating residues pointing into a cleft on the BiP molecule. Synthetic peptides with sequences corresponding to those displayed by BiP-binding bacteriophages …

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Identification Of A Protein In Several Borrelia Species Which Is Related To Ospc Of The Lyme Disease Spirochetes., Richard T. Marconi, D. Scott Samuels, Tom G. Schwan, Claude F. Garon Oct 1993

Identification Of A Protein In Several Borrelia Species Which Is Related To Ospc Of The Lyme Disease Spirochetes., Richard T. Marconi, D. Scott Samuels, Tom G. Schwan, Claude F. Garon

Biological Sciences Faculty Publications

Using oligonucleotide probes which have previously been shown to be specific for the ospC gene found in the Lyme disease spirochete species Borrelia burgdorferi, B. garinii, and group VS461, we detected an ospC homolog in other Borrelia species including B. coriaceae, B. hermsii, B. anserina, B. turicatae, and B. parkeri. In contrast to the Lyme disease spirochetes, which carry the ospC gene on a 26-kb circular plasmid, we mapped the gene in other Borrelia species to linear plasmids which varied in size among the isolates tested. Some isolates carry multiple copies of the gene residing on linear plasmids of different …

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On A (Β-) Roll, S. R. Sprang Sep 1993

On A (Β-) Roll, S. R. Sprang

Biological Sciences Faculty Publications

No abstract provided.

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Nucleotide Sequence And Comparison Of The 5s Ribosomal Rna Genes Of Rochalimaea Henselae, R-Quintana And Brucella Abortus, Michael F. Minnick, Gary L. Stiegler May 1993

Nucleotide Sequence And Comparison Of The 5s Ribosomal Rna Genes Of Rochalimaea Henselae, R-Quintana And Brucella Abortus, Michael F. Minnick, Gary L. Stiegler

Biological Sciences Faculty Publications

No abstract provided.

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Breeding Ground Fidelity And Mate Retention In The Pacific Golden-Plover, Oscar W. Johnson, Peter G. Connors, Phillip L. Bruner, John L. Maron Mar 1993

Breeding Ground Fidelity And Mate Retention In The Pacific Golden-Plover, Oscar W. Johnson, Peter G. Connors, Phillip L. Bruner, John L. Maron

Biological Sciences Faculty Publications

We found male-biased site fidelity in Pacific Golden-Plovers (Phviah fulva)
on breeding grounds in western Alaska. Males (8 of 8) returned to the same territories annually, while few females (1 of 4) were seen in subsequent seasons. Nest sites in successive years were usually within 100 m, and the same nest cup may be used in more than one year. First-year birds of both sexes mated with older birds and also with each other, but first-year females may breed less commonly than first-year males. The numbers of nesting birds on our study sites varied sharply both within and …

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Nucleotide Sequence Of The 5s Ribosomal Rna Gene Of Bartonella Bacilliformis, Michael F. Minnick Feb 1993

Nucleotide Sequence Of The 5s Ribosomal Rna Gene Of Bartonella Bacilliformis, Michael F. Minnick

Biological Sciences Faculty Publications

No abstract provided.

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