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Articles 871 - 900 of 1681
Full-Text Articles in Biology
Pcr Screening For The Surfactin (Sfp) Gene In Marine Bacillus Strains And Its Molecular Characterization From Bacillus Tequilensis Nios11, Seema Porob, Sagar Nayak, Areena Fernandes, Priyanka Padmanabhan, Balkrishna Arjun Patil, Ram Murti Meena, Nagappa Ramaiah
Pcr Screening For The Surfactin (Sfp) Gene In Marine Bacillus Strains And Its Molecular Characterization From Bacillus Tequilensis Nios11, Seema Porob, Sagar Nayak, Areena Fernandes, Priyanka Padmanabhan, Balkrishna Arjun Patil, Ram Murti Meena, Nagappa Ramaiah
Turkish Journal of Biology
The sfp gene responsible for surfactin production was screened from the DNA extracts of 37 Bacillus spp. whose identity was confirmed by 16S rRNA gene sequence analyses. PCR screening revealed amplification of sfp gene fragments in a total of 25 isolates. Several isolates belonging to Bacillus tequilensis were found to be positive for this gene. A gene fragment coding for the sfp gene was amplified and cloned from genomic DNA of the isolate B. tequilensis NIOS11. The cloned gene has an open reading frame of 675 bp coding for 224 amino acids with a theoretical molecular weight of 26,166.33 Da …
Memory-Enhancing Effects Of The Leptin Hormone In Wistar Albino Rats: Sex And Generation Differences, Alper Karakaş, Hami̇t Coşkun, Fevzi̇ye Umut Kizilkaya
Memory-Enhancing Effects Of The Leptin Hormone In Wistar Albino Rats: Sex And Generation Differences, Alper Karakaş, Hami̇t Coşkun, Fevzi̇ye Umut Kizilkaya
Turkish Journal of Biology
Leptin is a hormone secreted by adipose tissue that informs the brain about the fat stores of the body. In this study, we examined the effects of timed leptin injections on spatial memory performances of adult and juvenile male and female Wistar rats with the Morris water maze test. We applied the injections and conducted the training trials for 4 days. On the fifth day after leptin injections, the experiments were performed. The activities were recorded and analyzed with the Noldus Ethovision Animal Video Tracking System. The results show that the spatial memory performance of the rats was improved by …
An Ethnobotanical Survey In Selected Towns Of The Mediterranean Subregion (Turkey), Gali̇p Akaydin, Işil Şi̇mşek, Zeki̇ye Ceren Arituluk, Erdem Yeşi̇lada
An Ethnobotanical Survey In Selected Towns Of The Mediterranean Subregion (Turkey), Gali̇p Akaydin, Işil Şi̇mşek, Zeki̇ye Ceren Arituluk, Erdem Yeşi̇lada
Turkish Journal of Biology
This survey was carried out by face-to-face oral interviews with 379 inhabitants who agreed to be interviewed in selected localities of the Mediterranean region: Hatay (İskenderun and Narlıca), İçel (Erdemli, Tarsus, and Silifke), and Isparta (Sav). The current study was conducted to document the ethnobotanical uses of plants. In the first step of the study, demographic profiles of the informants were evaluated. Subsequently, they were asked to state whether the plants were used for therapeutic or other purposes; then detailed information, i.e. local names, parts used, methods of preparation (decoction, infusion, poultice, ointment, etc.) was recorded; and finally the plant …
Molecular Characterization Of Yarrowia Lipolytica Strains Isolated From Different Environments And Lipase Profiling, Onur Akpinar, Füsun Bahri̇ye Uçar
Molecular Characterization Of Yarrowia Lipolytica Strains Isolated From Different Environments And Lipase Profiling, Onur Akpinar, Füsun Bahri̇ye Uçar
Turkish Journal of Biology
In order to analyze yeasts that produce industrial compounds, it is essential to identify them accurately. Yarrowia lipolytica is one of the most extensively studied 'nonconventional' yeasts, being a strictly aerobic microorganism capable of producing important metabolites and having an intense secretory activity, which justifies efforts to use it in industry (as a biocatalyst), in molecular biology, and in studies of genetics. Therefore, in this study, an accurate identification of Y. lipolytica strains was performed using 3 different molecular biological methods (RFLP analysis of ITS1-5.8S rDNA-ITS2 and 18S rDNA regions and sequencing of the D1/D2 domain of the 26S rDNA …
Biopolymer Generation From Sweet Sorghum Juice: Screening, Isolation, Identification, And Fermentative Polyhydroxyalkanoate Production By Bacillus Aryabhattai, Varavut Tanamool, Tsuyoshi Imai, Paiboon Danvirutai, Pakawadee Kaewkannetra
Biopolymer Generation From Sweet Sorghum Juice: Screening, Isolation, Identification, And Fermentative Polyhydroxyalkanoate Production By Bacillus Aryabhattai, Varavut Tanamool, Tsuyoshi Imai, Paiboon Danvirutai, Pakawadee Kaewkannetra
Turkish Journal of Biology
This study aims to use sweet sorghum juice (SSJ) to produce an environmentally friendly biopolymer of polyhydroxyalkanoates (PHAs) by bacteria isolated from soil. Among 18 isolates, only 3 isolates, coded as S4, S6, and S11, were chosen to produce PHA via batch fermentation compared to 3 strains of Alcaligenes eutrophus, A. latus, and Hydrogenophaga sp., respectively, under controlled conditions at pH 7, 35 °C, and 20 g/L of initial total sugar in the SSJ. The results clearly showed that the isolate S4 can produce maximum PHAs of 1.74 g/L with 57.62% dry cell weight and yield maximum hourly productivity at …
Evaluation Of E330-Induced Developmental Toxicity Using Fetax, Ayper Boğa Pekmezekmek, Uğur Seçi̇l Bi̇nokay, Kübra Akillioğlu, Yaşar Sertdemi̇r
Evaluation Of E330-Induced Developmental Toxicity Using Fetax, Ayper Boğa Pekmezekmek, Uğur Seçi̇l Bi̇nokay, Kübra Akillioğlu, Yaşar Sertdemi̇r
Turkish Journal of Biology
The present study evaluates the teratogenic and toxicological effects of citric acid (E330), a food additive, on Xenopus laevis embryos. The embryos were exposed to a range of E330 concentrations, from stage 8 to 11 of development, for 96 h under static renewal test conditions. The median lethal concentrations, no-observed adverse effect concentration (NOAEC), lowest observed adverse effect concentration (LOAEC), and minimum concentration to inhibit growth (MCIG) values were calculated. The lethal concentration (LC) values LC_{10}, LC_{20}, LC_{30}, LC_{40}, and LC_{50} determined for E330 exposure were 0.0113, 0.0117, 0.0119, 0.0122, and 0.0124 g/L, respectively. NOAEC and LOAEC values were calculated …
Evaluation Of The Genetic Relationship Between Fritillaria Species From Turkey’S Flora Using Fluorescent-Based Aflp, Özge Karakaş Meti̇n, Mi̇ne Türktaş, Meral Aslay, Erdal Kaya
Evaluation Of The Genetic Relationship Between Fritillaria Species From Turkey’S Flora Using Fluorescent-Based Aflp, Özge Karakaş Meti̇n, Mi̇ne Türktaş, Meral Aslay, Erdal Kaya
Turkish Journal of Biology
In this study, the genetic relationships among 12 Fritillaria taxa collected from different regions of Turkey were investigated using the amplified fragment length polymorphism (AFLP) technique. Seven primer pair combinations were used for the analysis. The neighbor-joining and principal coordinate analysis methods were implemented to identify the taxonomy of the species. The analyses generated congruent results with each other and with the previous studies. The neighbor-joining analysis clustered the species into 3 groups, while the principal coordinates analysis did not support separation of the third group. The subgenera Fritillaria and Petilium diverged from each other with strong support in all …
Medium Optimization For The Production Of Exopolysaccharide By Bacillus Subtilis Using Synthetic Sources And Agro Wastes, Sirajunnisa Abdul Razack, Vijayagopal Velayutham, Viruthagiri Thangavelu
Medium Optimization For The Production Of Exopolysaccharide By Bacillus Subtilis Using Synthetic Sources And Agro Wastes, Sirajunnisa Abdul Razack, Vijayagopal Velayutham, Viruthagiri Thangavelu
Turkish Journal of Biology
Exopolysaccharides (EPSs) play an extensive role as biopolymers in the environment by replacing synthetic polymers as they are degradable, nontoxic, and produced by microorganisms. An attempt was made to optimize a medium, by the one-factor-at-a-time method, for an enhanced production of EPS from a soil isolate, Bacillus subtilis. The study was carried out by experimenting on various nutrients at different concentrations. EPS was precipitated using ethanol, the total carbohydrate content was determined by phenol sulfuric acid method, and functional groups were detected by Fourier transform infrared (FTIR) spectrophotometry. The finalized medium contained sucrose (20 g/L), yeast extract (5 g/L), NaCl …
Molecular Detection Of Antibiotic Related Genes From Pseudomonas Aeruginosa Fp6, An Antagonist Towards Rhizoctonia Solani And Colletotrichum Gloeosporioides, Sasirekha Bakthavatchalu, Srividya Shivakumar, Shankar Bhat Sullia
Molecular Detection Of Antibiotic Related Genes From Pseudomonas Aeruginosa Fp6, An Antagonist Towards Rhizoctonia Solani And Colletotrichum Gloeosporioides, Sasirekha Bakthavatchalu, Srividya Shivakumar, Shankar Bhat Sullia
Turkish Journal of Biology
Despite the importance of antibiosis in biological control, little is known about the genes involved in antifungal activity. Therefore, the present study was aimed at identifying the location of the antagonistic gene(s) in Pseudomonas aeruginosa FP6 towards Rhizoctonia solani and Colletotrichum gloeosporioides using a PCR-based approach. A new bacterial strain, designated as FP6, was isolated from rhizospheric soil and identified as a member of Pseudomonas aeruginosa based on 16S rRNA analysis. The secondary metabolites produced by this strain have shown broad-spectrum antifungal activity against Rhizoctonia solani and Colletotrichum gloeosporioides. Antifungal metabolites of a nonenzymatic nature were found to be responsible …
Effects Of Acacia Cyanophylla Lindl. Extracts On Seed Germination And Seedling Growth Of Four Crop And Weed Plants, Asma El Ayeb, Hichem Ben Jannet, Fethia Harzallah Skhiri
Effects Of Acacia Cyanophylla Lindl. Extracts On Seed Germination And Seedling Growth Of Four Crop And Weed Plants, Asma El Ayeb, Hichem Ben Jannet, Fethia Harzallah Skhiri
Turkish Journal of Biology
The aqueous and organic extracts of the roots, stems, phyllodes, flowers, legumes, and seeds of Acacia cyanophylla Lindl. were assayed at different concentrations to assess their allelopathic potential. The extracts were tested on the seeds of 2 crops (Triticum aestivum L. and Lactuca sativa L.) and 2 weeds (Peganum harmala L. and Silybum marianum L.) species. The final germination percentages and the seedling shoot and root lengths were significantly reduced by the A. cyanophylla extracts as compared to the control. Aqueous extract from seeds was the strongest inhibitor of germination, shoot length, and root length for L. sativa and P. …
Cr(Vi) Reduction By Cellulosimicrobium Sp. Isolated From Tannery Effluent, Aniqa Naeem, Rida Batool, Nazia Jamil
Cr(Vi) Reduction By Cellulosimicrobium Sp. Isolated From Tannery Effluent, Aniqa Naeem, Rida Batool, Nazia Jamil
Turkish Journal of Biology
Cr(VI) is a toxic metal that has carcinogenic and mutagenic effects on all living organisms. In order to study the contribution of microbes towards Cr(VI) reduction into the less toxic Cr(III) form, an indigenous chromium-resistant bacterial strain (A_8) was isolated from a tannery effluent. It was identified as Cellulosimicrobium sp. on the basis of morphological and biochemical characterization and 16S rRNA gene sequencing. It could tolerate up to 1800 µg mL^{-1} of K_{2}CrO_{4} and showed an optimum reduction (98.6%) of Cr(VI) at the concentration of 900 µg mL^{-1} aerobically. Cell-free enzyme assay exhibited the reduction of Cr(VI) in vitro. A …
Enhancement Of Mycolytic Activity Of An Antagonistic Bacillus Subtilis Through Ethyl Methane Sulfonate (Ems) Mutagenesis, Ashwini Narasimhan, Samantha Suresh, Deepak Bist, Srividya Shivakumar
Enhancement Of Mycolytic Activity Of An Antagonistic Bacillus Subtilis Through Ethyl Methane Sulfonate (Ems) Mutagenesis, Ashwini Narasimhan, Samantha Suresh, Deepak Bist, Srividya Shivakumar
Turkish Journal of Biology
Bacillus subtilis, isolated from the chili rhizosphere, capable of producing 3 mycolytic enzymes (chitinase, \beta-1,3-glucanase, and cellulase), was mutated by the chemical mutagen ethyl methane sulfonate (EMS). Mutants were screened based on their antifungal ability on dual plate assay against Colletotrichum gloeosporioides OGC1, the causal agent of anthracnose disease in fruit crops. EMS mutagenesis yielded 60 isolated mutants on NA plates, of which 3 (M3, M4, and M24) showed loss of antagonism against C. gloeosporioides OGC1 and 6 (M8, M21, M22, M57, M58, and M59) exhibited increased antagonism. The remaining mutants did not show any difference in their antagonistic property. …
In Vitro Antimicrobial And Cytotoxic Activity Of Tamarix Dioica Roxb. Leaves, Saifullah Khan, Farman Ullah, Tahir Mahmood
In Vitro Antimicrobial And Cytotoxic Activity Of Tamarix Dioica Roxb. Leaves, Saifullah Khan, Farman Ullah, Tahir Mahmood
Turkish Journal of Biology
Medicinal plants have been in use for thousands of years for treatment of different types of diseases and a large inventory of useful drugs has been isolated from them. The aim of our study is to investigate a new and effective plant possessing antimicrobial activity. The crude extracts of Tamarix dioica Roxb. leaves, as well as fractions of the extract in various solvents, were investigated against standard strains of bacteria Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Shigella flexneri, Bacillus subtilis, Salmonella typhi, and Staphylococcus aureus and fungi Candida glabrata, Aspergillus niger, Trichophyton rubrum, Candida tropicalis, and Aspergillus fumigatus. For in …
The Effect Of Hydrogen Peroxide/Colloidal Silver On Reducing The Colonization And Growth Of Heterotrophic Bacteria In Dental Unit Waterlines, Meral Özalp, Ömer Engi̇n Bulut, Ati̇lla Stephan Ataç, Meli̇ke Eki̇zoğlu, Di̇dem Kart, Hakan Hamdi̇ Çeli̇k, İlkan Tatar
The Effect Of Hydrogen Peroxide/Colloidal Silver On Reducing The Colonization And Growth Of Heterotrophic Bacteria In Dental Unit Waterlines, Meral Özalp, Ömer Engi̇n Bulut, Ati̇lla Stephan Ataç, Meli̇ke Eki̇zoğlu, Di̇dem Kart, Hakan Hamdi̇ Çeli̇k, İlkan Tatar
Turkish Journal of Biology
The objective of the present study was to investigate reduction in the colonization and growth of heterotrophic bacteria in dental unit waterlines by using hydrogen peroxide/colloidal silver as disinfectant. Twenty-seven dental units were included; 6 units that were more than 20 years old and 7 units that had been in use for 2 years comprised the old and new treatment groups, respectively. Fourteen units served as controls. The treatment groups were disinfected continuously and every 4 weeks shock doses were applied over a 20-week period. Water samples were taken before treatment, 1 and 2 weeks after treatment, and thereafter every …
Role Of Plant Growth Promoting Rhizobacteria On Antioxidant Enzyme Activities And Tropane Alkaloid Production Of Hyoscyamus Niger Under Water Deficit Stress, Mansour Ghorbanpour, Mehrnaz Hatami, Kazem Khavazi
Role Of Plant Growth Promoting Rhizobacteria On Antioxidant Enzyme Activities And Tropane Alkaloid Production Of Hyoscyamus Niger Under Water Deficit Stress, Mansour Ghorbanpour, Mehrnaz Hatami, Kazem Khavazi
Turkish Journal of Biology
This study examined the effects of inoculation with 2 rhizobacteria strains, Pseudomonas putida (PP) and Pseudomonas fluorescens (PF), on growth parameters, chlorophyll, proline, leaf relative water content (RWC), antioxidant enzyme activities (including superoxide dismutase (SOD), peroxidase (POX), and catalase (CAT)), tropane alkaloids (such as hyoscyamine (HYO) and scopolamine (SCO)), and production of Hyoscyamus niger under 3 water deficit stress (WDS) levels, i.e. 30% (W1), 60% (W2), and 90% (W3) water depletion of field capacity. The results showed that inoculation with PP and PF strains minimized the deleterious effects of WDS on growth parameters. The activities of SOD and POX in …
Effect Of Exogenously Applied Salicylic Acid On Cadmium Chloride-Induced Oxidative Stress And Nitrogen Metabolism In Tomato (Lycopersicon Esculentum L.), Esra Koç, Ayşen Sülün Üstün, Nuri̇ Çeli̇k
Effect Of Exogenously Applied Salicylic Acid On Cadmium Chloride-Induced Oxidative Stress And Nitrogen Metabolism In Tomato (Lycopersicon Esculentum L.), Esra Koç, Ayşen Sülün Üstün, Nuri̇ Çeli̇k
Turkish Journal of Biology
Salicylic acid (SA) has a key role in the formation of induced systemic resistance and hypersensitive cells, but it has also been indicated to be effective in the formation of strong responses against abiotic stresses such as drought, cold, heavy metal toxicity, and osmotic stress. This study has demonstrated a connection between the total soluble protein and the nitrate reductase (NR) activity. In 20 \muM CdCl_2, 40 \muM CdCl_2 + 0.5 mM SA, and 100 \muM CdCl_2 + 0.5 mM SA treatments, on the 1st day following the treatment an increase was detected in the NR activity when compared to …
A Cellulase-Free, Thermoactive, And Alkali Xylanase Production By Terrestrial Streptomyces Sp. Ca24, İlknur Porsuk, Süleyman Özakin, Bülent Bali̇, Ebru İnce Yilmaz
A Cellulase-Free, Thermoactive, And Alkali Xylanase Production By Terrestrial Streptomyces Sp. Ca24, İlknur Porsuk, Süleyman Özakin, Bülent Bali̇, Ebru İnce Yilmaz
Turkish Journal of Biology
Microbial xylanases have attracted much attention because of their applications in industrial processes during the past few decades. Sixty-five terrestrial actinomycetes previously isolated from rhizospheric soils of endemic plants were tested for their ability in production of xylanase. The results revealed that 33 isolates could degrade xylan on both solid and liquid media. Extracellular xylanase production by these isolates was characterized during their growth in liquid medium. Streptomyces sp. CA24, one of the most active strains, was selected for optimization of xylanase production. The enzyme secretion was enhanced when the medium was supplemented with oat spelt xylan (0.5%) and yeast …
Purification And Characterization Of A Novel Detergent- And Organic Solvent-Resistant Endo-Beta-1,4-Glucanase From A Newly Isolated Basidiomycete Peniophora Sp. Ndvn01, Dinh Kha Trinh, Dinh Thi Quyen, Thi Tuyen Do, Ngoc Minh Nghiem
Purification And Characterization Of A Novel Detergent- And Organic Solvent-Resistant Endo-Beta-1,4-Glucanase From A Newly Isolated Basidiomycete Peniophora Sp. Ndvn01, Dinh Kha Trinh, Dinh Thi Quyen, Thi Tuyen Do, Ngoc Minh Nghiem
Turkish Journal of Biology
A novel extracellular endoglucanase from a basidiomycete strain Peniophora sp. NDVN01 was purified 2.8-fold to homogeneity through ammonium sulfate precipitation and gel filtration with Bio-Gel P-100 and Sephadex G-75. The endoglucanase had a specific activity of 163.8 U/mg protein and a molecular mass of 32 kDa. Optimum temperature and pH were at 60 °C and 4.5, respectively. The enzyme was stable at up to 42 °C and in the pH range of 3.5-5.5 with a residual activity of over 80% for 24 h of treatment. Ni^2^+ activated but other metal ions showed no or slight inhibitory effect on the enzyme …
Characterization Of A New Isolate Of Malacosoma Neustria Nucleopolyhedrovirus (Manenpv) From Turkey, İsmai̇l Demi̇r, Remzi̇ye Nalçacioğlu, Li̇da Mohammad Gholi̇zad, Zi̇hni̇ Demi̇rbağ
Characterization Of A New Isolate Of Malacosoma Neustria Nucleopolyhedrovirus (Manenpv) From Turkey, İsmai̇l Demi̇r, Remzi̇ye Nalçacioğlu, Li̇da Mohammad Gholi̇zad, Zi̇hni̇ Demi̇rbağ
Turkish Journal of Biology
The lackey moth, Malacosoma neustria (Linnaeus, 1758), a worldwide pest, causes extensive economic losses on particularly hazelnut, Prunus, Quercus, Populus, and Salix trees. In this study, a local nucleopolyhedrovirus (NPV) was isolated from the larvae of M. neustria in the northeast of Turkey. It was named ManeNPV-T2. Electron microscopic observations showed that the polyhedra of the new isolate contain several virions with multiple nucleocapsids packaged within a single viral envelope. Restriction endonuclease analysis of ManeNPV-T2 DNA indicated that it has a different restriction profile compared to the previous Turkish and Latvian isolates. The phylogenetic analysis of the amplified polh sequence …
Optimization Of Immobilization Conditions By Conventional And Statistical Strategies For Alkaline Lipase Production By Pseudomonas Aeruginosa Mutant Cells: Scale-Up At Bench-Scale Bioreactor Level, Deepali Bisht, Santosh Kumar Yadav, Nandan Singh Darmwal
Optimization Of Immobilization Conditions By Conventional And Statistical Strategies For Alkaline Lipase Production By Pseudomonas Aeruginosa Mutant Cells: Scale-Up At Bench-Scale Bioreactor Level, Deepali Bisht, Santosh Kumar Yadav, Nandan Singh Darmwal
Turkish Journal of Biology
Suitability of 3 matrices, agarose, sodium alginate, and polyacrylamide, for immobilization of mutant cells of Pseudomonas aeruginosa MTCC 10,055 was investigated. Of these, agarose was proven to be the best as exhibiting maximum enzyme production (4363.4 U/mL), followed by polyacrylamide gel (2172.3 U/mL). Alginate beads were the poorest. The one-variable-at-a-time approach suggested agarose at 2.0%, immobilized bead at 4.0 g blocks/50 mL, and initial cell loading of 0.8 g in the matrix as optimum conditions for maximum lipase production (5982.3 U/mL) after 24 h of incubation. However, response surface methodology studies determined the optimum values of these variables as 1.96%, …
Investigation Of Ll-37-Mediated Sirna Transfection, Mustafa Yalçinkaya, Şahru Yüksel
Investigation Of Ll-37-Mediated Sirna Transfection, Mustafa Yalçinkaya, Şahru Yüksel
Turkish Journal of Biology
RNA interference is not only an important tool for studying protein function; it also holds many promises for the treatment of a variety of diseases in which the function of unwanted proteins needs to be suppressed. For small interfering RNA (siRNA) to be used as a therapeutic agent, the biggest hurdle is to find the right delivery agent that is both nontoxic to the cells and efficient in delivery. Synthetic and natural peptides from a variety of organisms have been investigated for this purpose. However, there is still no agent or formulation that has been approved for siRNA delivery. Antimicrobial …
Phospholipases C From Pseudomonas Aeruginosa And Bacillus Cereus Isolates, Chromosome-Mediated Enzymes With Roles In Virulence, Nooran Elleboudy, Mohammad Aboulwafa, Nadia Hassouna
Phospholipases C From Pseudomonas Aeruginosa And Bacillus Cereus Isolates, Chromosome-Mediated Enzymes With Roles In Virulence, Nooran Elleboudy, Mohammad Aboulwafa, Nadia Hassouna
Turkish Journal of Biology
Phospholipases C (PLCs) from Pseudomonas aeruginosa D183 and Bacillus cereus D101, 2 clinical isolates from 2 pus specimens, were partially purified by ammonium sulfate precipitation followed by dialysis and used to study the possible role of PLC in the virulence of the isolates. Partially purified PLC from both isolates induced lysis of Vero cells in the presence and absence of the producing bacterial cells. Noncytolytic dilutions of the partially purified PLC from Pseudomonas aeruginosa increased adherence of the producing cells to Vero cells but did not affect internalization. However, Bacillus cereus cells neither adhered to nor were internalized within the …
Occurrence Of Enterocin Genes, Virulence Factors, And Antibiotic Resistance In 3 Bacteriocin-Producer Enterococcus Faecium Strains Isolated From Turkish Tulum Cheese, Banu Özden Tuncer, Zeli̇ha Ay, Yasi̇n Tuncer
Occurrence Of Enterocin Genes, Virulence Factors, And Antibiotic Resistance In 3 Bacteriocin-Producer Enterococcus Faecium Strains Isolated From Turkish Tulum Cheese, Banu Özden Tuncer, Zeli̇ha Ay, Yasi̇n Tuncer
Turkish Journal of Biology
In this study, the occurrence of enterocin genes, virulence factors, and antibiotic resistance in the bacteriocin-producer Enterococcus faecium EYT17, EYT31, and EYT39 strains was investigated. Polymerase chain reaction (PCR) studies showed that all of the E. faecium strains carried the enterocin A and B structural genes (entA and entB), while E. faecium EYT17 and EYT31 strains also carried the enterocin P structural gene (entP). None of the strains exhibited \beta-haemolysis or gelatinase activity. PCR analysis revealed that EYT17, EYT31, and EYT39 strains were clear of potential virulence determinants, except for ccf and efaAfm. The ccf gene was found in all …
The Utilization Of Lignocellulosic Wastes For Laccase Production Under Semisolid-State And Submerged Fermentation Conditions, Emre Bi̇rhanli, Özfer Yeşi̇lada
The Utilization Of Lignocellulosic Wastes For Laccase Production Under Semisolid-State And Submerged Fermentation Conditions, Emre Bi̇rhanli, Özfer Yeşi̇lada
Turkish Journal of Biology
The aim of this study was to produce laccase enzymes by using various lignocellulosic wastes (LCWs) under semisolid-state (SsF) and submerged fermentation (SF) conditions. White rot fungi are the best laccase-producing organisms and they can be easily grown on LCW. Thus, in this work, Trametes trogii (Berk.) ATCC 200800 and Trametes versicolor (L.) ATCC 200801, well-known laccase-producing white rot fungi, were used as laccase-producing organisms. According to the literature, some of the LCWs such as sunflower receptacle, apricot seed shell, and bulrush were tested first as substrates for laccase production by these fungi. In SsF, the maximum laccase activities were …
Stimulation Of Dendritic Cells With Vaccine And Vaccine-Antibody Complex And Effect On Immune Response, İbrahi̇m Hati̇poğlu, İbrahi̇m Söğüt, Duygu Ercan, Soner Aksu, Hülya Si̇vas, Aynur Başalp
Stimulation Of Dendritic Cells With Vaccine And Vaccine-Antibody Complex And Effect On Immune Response, İbrahi̇m Hati̇poğlu, İbrahi̇m Söğüt, Duygu Ercan, Soner Aksu, Hülya Si̇vas, Aynur Başalp
Turkish Journal of Biology
Dendritic cell (DC) vaccines are a promising and potent therapeutic tool for chronic diseases, autoimmune diseases, and cancer because of the unique ability of DCs to stimulate T cells. The challenge of DC vaccines is to find an effective form for antigen presentation. Although pure antigens, antigen complexes, plasmids, and mRNA have been used in different studies, no proper application to overcome this problem has been found yet. In this study, we investigated the eligibility of a commercial hepatitis B virus (HBV) vaccine or a vaccine-monoclonal antibody complex for antigen loading of DCs for a therapeutic purpose. DCs were derived …
Pomegranate (Punica Granatum L.) Reduces Endoplasmic Reticulum Stress Induced By Renal Ischemia/Reperfusion Injury In Rat, Khadijeh Hashemi, Kazem Parivar, Samira Fazlifar, Alireza Haghparast, Mehrdad Mohri, Naser Margan Azghadi, Mohammadhossein Nazem Shirazi, Amir Afkhami Goli
Pomegranate (Punica Granatum L.) Reduces Endoplasmic Reticulum Stress Induced By Renal Ischemia/Reperfusion Injury In Rat, Khadijeh Hashemi, Kazem Parivar, Samira Fazlifar, Alireza Haghparast, Mehrdad Mohri, Naser Margan Azghadi, Mohammadhossein Nazem Shirazi, Amir Afkhami Goli
Turkish Journal of Biology
Ischemia/reperfusion (I/R) is one of the main causes of acute renal failure, leading to generation of reactive oxygen species (ROS) and ensuing oxidative stress, which results in unfolded/misfolded protein accumulation and its dependent response pathways, generally known as unfolded protein response (UPR), in the endoplasmic reticulum (ER). We studied the effects of renal I/R on the expression of ER-stress genes, as well as concomitant effects of oral pretreatment with pomegranate (Punica granatum L.) pith and carpellary membrane (PPCM). An in vivo model of rat kidney I/R followed by conventional and real-time RT-PCR was used to evaluate the modulation of GRP78 …
Production Of Herbicide-Resistant Cowpea (Vigna Unguiculata L.) Transformed With The Bar Gene, Muhammad Aasim, Khalid Mahmood Khawar, Sebahatti̇n Özcan
Production Of Herbicide-Resistant Cowpea (Vigna Unguiculata L.) Transformed With The Bar Gene, Muhammad Aasim, Khalid Mahmood Khawar, Sebahatti̇n Özcan
Turkish Journal of Biology
Plant genetic engineering has opened new avenues to modify crops and has provided a powerful tool for crop improvement. The present study reports the development of regeneration and genetic transformation protocol for the Turkish cowpea cultivar Akkiz. The immature cotyledons were cultured on Murashige and Skoog (MS) medium containing 0.25-0.75 mg L^{-1} 6-benzylaminopurine with or without 0.25 mg L^{-1} \alpha-naphthalene acetic acid. Shoot regeneration varied 44.4%-83.3% with 2.1-5.0 shoots per explant. Regenerated shoots were rooted in MS medium containing 0.50 mg L^{-1} indole-3-butyric acid and acclimatized in the greenhouse, where they flowered and set seeds. Immature cotyledons were transformed with …
Assessment Of Genetic Diversity In Naturally Growing 29 Trifolium L. Taxa From Bolu Province Using Rapd And Ssr Markers, Emel Uslu, Gözde Damla Ertuğrul, Mehmet Teki̇n Babaç
Assessment Of Genetic Diversity In Naturally Growing 29 Trifolium L. Taxa From Bolu Province Using Rapd And Ssr Markers, Emel Uslu, Gözde Damla Ertuğrul, Mehmet Teki̇n Babaç
Turkish Journal of Biology
Two molecular marker technologies, RAPD and SSR, were used to determine the genetic diversity of 29 taxa of Trifolium L. collected from Bolu Province. The analysis was carried out using 11 RAPD and 5 SSR markers. A total of 446 and 331 fragments were produced by RAPD and SSR markers, respectively. The amplification products were 300-3500 bp with RAPD and 125-3000 bp with SSR markers. These bands were scored as absence and presence for all taxa. Data obtained were used to estimate genetic similarity using Jaccards coefficient, and dendrograms were constructed by the UPGMA method using either RAPD and SSR …
Effect Of Drought Stress On Oxidative Damage And Antioxidant Enzyme Activity In Melon Seedlings, Musa Kavas, Mehmet Cengi̇z Baloğlu, Oya Akça, Fatma Seli̇n Köse, Derya Gökçay
Effect Of Drought Stress On Oxidative Damage And Antioxidant Enzyme Activity In Melon Seedlings, Musa Kavas, Mehmet Cengi̇z Baloğlu, Oya Akça, Fatma Seli̇n Köse, Derya Gökçay
Turkish Journal of Biology
Drought stress is one of the most serious environmental limitations affecting the growth and productivity of plants. In the current study, oxidative damage and antioxidant responses under polyethylene glycol (PEG)-induced osmotic stress were compared in 2 melon cultivars, Kırkağaç and Galia. Melon seedlings were subjected to PEG-6000 solutions of 2 different osmotic potentials, -0.2 MPa and -0.4 MPa. Various physiological parameters, malondialdehyde (MDA), proline content, and antioxidant enzymes including catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) were measured. A reduction in fresh and dry weights of shoot and root tissues was observed. Significant proline accumulation was detected with …
Effect Of Genotypical Factors On The Effectiveness Of Anther Culture In Eggplant (Solanum Melongena L.), Sevi̇nç Başay, Şeküre Şebnem Elli̇altioğlu
Effect Of Genotypical Factors On The Effectiveness Of Anther Culture In Eggplant (Solanum Melongena L.), Sevi̇nç Başay, Şeküre Şebnem Elli̇altioğlu
Turkish Journal of Biology
The aim of this study was to determine the androgenic capacity of some eggplant (Solanum melongena L.) varieties and breeding lines, and to establish the effectiveness of androgenesis induction in the less responsive genotypes by crossing with more responsive genotypes that have a high ability for haploid embryo formation. Flower buds containing greenish-yellow anthers were collected when the microspores were at the late uninucleate stage, which is equivalent to a bud size where petals are not visible. The anthers were kept in the dark at 35 °C for 8 days, then incubated with a 12 h light period at 25 …